| oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions. | the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ... | 2015 | 26399712 |
| heat sensitivity of wmel wolbachia during aedes aegypti development. | the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ... | 2016 | 27459519 |
| reciprocal tripartite interactions between the aedes aegypti midgut microbiota, innate immune system and dengue virus influences vector competence. | dengue virus is one of the most important arboviral pathogens and the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. it is transmitted between humans by the mosquitoes aedes aegypti and aedes albopictus, and at least 2.5 billion people are at daily risk of infection. during their lifecycle, mosquitoes are exposed to a variety of microbes, some of which are needed for their successful development into adulthood. however, recent studies have suggested that th ... | 2012 | 22413032 |
| wolbachia-associated bacterial protection in the mosquito aedes aegypti. | wolbachia infections confer protection for their insect hosts against a range of pathogens including bacteria, viruses, nematodes and the malaria parasite. a single mechanism that might explain this broad-based pathogen protection is immune priming, in which the presence of the symbiont upregulates the basal immune response, preparing the insect to defend against subsequent pathogen infection. a study that compared natural wolbachia infections in drosophila melanogaster with the mosquito vector ... | 2013 | 23951381 |
| serratia odorifera a midgut inhabitant of aedes aegypti mosquito enhances its susceptibility to dengue-2 virus. | mosquito midgut plays a crucial role in its vector susceptibility and pathogen interaction. identification of the sustainable microflora of the midgut environment can therefore help in evaluating its contribution in mosquito-pathogen interaction and in turn vector competence. to understand the bacterial diversity in the midgut of aedes aegypti mosquitoes, we conducted a screening study of the gut microbes of these mosquitoes which were either collected from fields or reared in the laboratory "cu ... | 2012 | 22848375 |
| chromobacterium csp_p reduces malaria and dengue infection in vector mosquitoes and has entomopathogenic and in vitro anti-pathogen activities. | plasmodium and dengue virus, the causative agents of the two most devastating vector-borne diseases, malaria and dengue, are transmitted by the two most important mosquito vectors, anopheles gambiae and aedes aegypti, respectively. insect-bacteria associations have been shown to influence vector competence for human pathogens through multi-faceted actions that include the elicitation of the insect immune system, pathogen sequestration by microbes, and bacteria-produced anti-pathogenic factors. t ... | 2014 | 25340821 |
| species composition of bacterial communities influences attraction of mosquitoes to experimental plant infusions. | in the container habitats of immature mosquitoes, catabolism of plant matter and other organic detritus by microbial organisms produces metabolites that mediate the oviposition behavior of aedes aegypti and aedes albopictus. public health agencies commonly use oviposition traps containing plant infusions for monitoring populations of these mosquito species, which are global vectors of dengue viruses. in laboratory experiments, gravid females exhibited significantly diminished responses to experi ... | 2009 | 19641948 |
| molecular characterization of midgut microbiota of aedes albopictus and aedes aegypti from arunachal pradesh, india. | microbiota inhabiting midguts of mosquitoes play a key role in the host - parasite interaction and enhance vectorial capacity of viral diseases like dengue and chikungunya fevers. mosquito midgut is considered to be an important site for host-pathogen interaction and pathogen survival is thought to be an outcome of this interaction. in the present study we examined the bacterial community in the midgut of aedes mosquitoes in arunanchal pradesh, india, a subtropical zone where dengue fever is rep ... | 2015 | 26684012 |
| design and testing of novel lethal ovitrap to reduce populations of aedes mosquitoes: community-based participatory research between industry, academia and communities in peru and thailand. | dengue virus (and chikungunya and zika viruses) is transmitted by aedes aegypti and aedes albopictus mosquitoes and causes considerable human morbidity and mortality. as there is currently no vaccine or chemoprophylaxis to protect people from dengue virus infection, vector control is the only viable option for disease prevention. the purpose of this paper is to illustrate the design and placement process for an attractive lethal ovitrap to reduce vector populations and to describe lessons learne ... | 2016 | 27532497 |
| heme signaling impacts global gene expression, immunity and dengue virus infectivity in aedes aegypti. | blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. heme is a pro-oxidant that influences a variety of cellular processes. we performed a global analysis of heme-regulated aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. we observed an iron- and reactive oxygen species (ros)-independent signaling induced by heme that comprised genes related to redox metabolis ... | 2015 | 26275150 |
| emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus. | in mammals, lipid droplets (lds) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. in insects, it is unknown whether lds play any role during the development of immune responses. we show that aedes aegypti aag2 cells - an immune responsive cell lineage - accumulates lds when challenged with enterobacter cloacae, sindbis, and dengue viruses. microarray analysis of aag2 challenged with e.cloacae or infected with dengue virus reveal ... | 2016 | 26887863 |
| impact of wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector aedes aegypti. | incidence of disease due to dengue (denv), chikungunya (chikv) and yellow fever (yfv) viruses is increasing in many parts of the world. the viruses are primarily transmitted by aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. when transinfected into ae. aegypti, the intracellular bacterium wolbachia has recently been shown to inhibit replication of denvs, chikv, malaria parasites and filarial nematodes, providing a potentially powerful biocontrol st ... | 2012 | 23133693 |
| risk of transmission of viral haemorrhagic fevers and the insecticide susceptibilitystatus of aedes aegypti (linnaeus) in some sites in accra, ghana. | dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. although, dengue has not been reported in ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease. | 0 | 27752187 |
| wolbachia biocontrol strategies for arboviral diseases and the potential influence of resident wolbachia strains in mosquitoes. | arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. the absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. the endosymbiotic bacterium wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. resident strains of wolbachia inhibit viral replication in drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows ... | 2016 | 26925368 |
| current status of invasive mosquito surveillance in the uk. | non-native invasive mosquitoes have for many years made incursions into europe, and are now established in many european countries. the continued european importation of potential vectors and their expansion within europe increases their potential for importation and establishment in the uk. coupled with increasing numbers of returning dengue and chikungunya infected travellers, the potential exists for transmission of vector borne disease in new regions. | 2015 | 26122427 |
| global climate change and its potential impact on disease transmission by salinity-tolerant mosquito vectors in coastal zones. | global climate change can potentially increase the transmission of mosquito vector-borne diseases such as malaria, lymphatic filariasis, and dengue in many parts of the world. these predictions are based on the effects of changing temperature, rainfall, and humidity on mosquito breeding and survival, the more rapid development of ingested pathogens in mosquitoes and the more frequent blood feeds at moderately higher ambient temperatures. an expansion of saline and brackish water bodies (water wi ... | 2012 | 22723781 |
| membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction. | understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ... | 2016 | 27083158 |
| larval temperature-food effects on adult mosquito infection and vertical transmission of dengue-1 virus. | temperature-food interactions in the larval environment can affect life history and population growth of container mosquitoes aedes aegypti (l.) and aedes albopictus skuse, the primary vectors of chikungunya and dengue viruses. we used ae. aegypti, ae. albopictus, and dengue-1 virus (denv-1) from florida to investigate whether larval rearing temperature can alter the effects of larval food levels on ae. aegypti and ae. albopictus life history and denv-1 infection and vertical transmission. altho ... | 2015 | 26489999 |
| host inflammatory response to mosquito bites enhances the severity of arbovirus infection. | aedes aegypti mosquitoes are responsible for transmitting many medically important viruses such as those that cause zika and dengue. the inoculation of viruses into mosquito bite sites is an important and common stage of all mosquito-borne virus infections. we show, using semliki forest virus and bunyamwera virus, that these viruses use this inflammatory niche to aid their replication and dissemination in vivo. mosquito bites were characterized by an edema that retained virus at the inoculation ... | 0 | 27332734 |
| isolation of midgut escape mutants of two american genotype dengue 2 viruses from aedes aegypti. | several studies have shown that american genotype dengue 2 viruses (denv2) have reduced viral fitness in the mosquito vector, aedes aegypti, compared to other denv2 genotypes. diminished replication efficiency or inability to efficiently traverse membrane barriers encompassing organs such as the midgut or salivary glands are considered major factors negatively impacting viral fitness in the mosquito. | 2013 | 23937713 |
| transgene-mediated suppression of the rna interference pathway in aedes aegypti interferes with gene silencing and enhances sindbis virus and dengue virus type 2 replication. | rna interference (rnai) is the major innate antiviral pathway in aedes aegypti that responds to replicating arboviruses such as dengue virus (denv) and sindbis virus (sinv). on the one hand, the mosquito's rnai machinery is capable of completely eliminating denv2 from ae. aegypti. on the other, transient silencing of key genes of the rnai pathway increases replication of sinv and denv2, allowing the viruses to temporally overcome dose-dependent midgut infection and midgut escape barriers (meb) m ... | 0 | 23331493 |
| specificity of resistance to dengue virus isolates is associated with genotypes of the mosquito antiviral gene dicer-2. | in contrast to the prevailing view that invertebrate immunity relies on broad-spectrum recognition and effector mechanisms, intrinsic genetic compatibility between invertebrate hosts and their pathogens is often highly specific in nature. solving this puzzle requires a better understanding of the molecular basis underlying observed patterns of invertebrate host-pathogen genetic specificity, broadly referred to as genotype-by-genotype interactions. here, we identify an invertebrate immune gene in ... | 0 | 23193131 |
| complex modulation of the aedes aegypti transcriptome in response to dengue virus infection. | dengue fever is the most important arboviral disease world-wide, with aedes aegypti being the major vector. interactions between the mosquito host and dengue viruses (denv) are complex and vector competence varies among geographically-distinct ae. aegypti populations. additionally, dengue is caused by four antigenically-distinct viral serotypes (denv1-4), each with multiple genotypes. each virus genotype interacts differently with vertebrate and invertebrate hosts. analyses of alterations in mos ... | 2012 | 23209765 |
| a novel coding-region rna element modulates infectious dengue virus particle production in both mammalian and mosquito cells and regulates viral replication in aedes aegypti mosquitoes. | dengue virus (denv) is an enveloped flavivirus with a positive-sense rna genome transmitted by aedes mosquitoes, causing the most important arthropod-borne viral disease affecting humans. relatively few cis-acting rna regulatory elements have been described in the denv coding-region. here, by introducing silent mutations into a denv-2 infectious clone, we identify the conserved capsid-coding region 1 (ccr1), an rna sequence element that regulates viral replication in mammalian cells and to a gre ... | 2012 | 22840606 |
| dengue virus infection of the aedes aegypti salivary gland and chemosensory apparatus induces genes that modulate infection and blood-feeding behavior. | the female aedes aegypti salivary gland plays a pivotal role in bloodmeal acquisition and reproduction, and thereby dengue virus (denv) transmission. it produces numerous immune factors, as well as immune-modulatory, vasodilatory, and anti-coagulant molecules that facilitate blood-feeding. to assess the impact of denv infection on salivary gland physiology and function, we performed a comparative genome-wide microarray analysis of the naïve and denv infection-responsive a. aegypti salivary gland ... | 2012 | 22479185 |
| genome investigations of vector competence in aedes aegypti to inform novel arbovirus disease control approaches. | dengue (denv), yellow fever, chikungunya, and zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, aedes aegypti. the "vectorial capacity" of a. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmi ... | 2016 | 27809220 |
| mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement. | dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels, aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α ... | 2016 | 27310141 |
| small rna profiling in dengue virus 2-infected aedes mosquito cells reveals viral pirnas and novel host mirnas. | in aedes mosquitoes, infections with arthropod-borne viruses (arboviruses) trigger or modulate the expression of various classes of viral and host-derived small rnas, including small interfering rnas (sirnas), piwi interacting rnas (pirnas), and micrornas (mirnas). viral sirnas are at the core of the antiviral rna interference machinery, one of the key pathways that limit virus replication in invertebrates. besides sirnas, aedes mosquitoes and cells derived from these insects produce arbovirus-d ... | 2016 | 26914027 |
| transstadial effects of bti on traits of aedes aegypti and infection with dengue virus. | most mosquito control efforts are primarily focused on reducing the adult population size mediated by reductions in the larval population, which should lower risk of disease transmission. although the aim of larviciding is to reduce larval abundance and thus recruitment of adults, nonlethal effects on adults are possible, including transstadial effects on phenotypes of adults such as survival and pathogen infection and transmission. in addition, the mortality induced by control efforts may act i ... | 2016 | 26871951 |
| a neuron-specific antiviral mechanism prevents lethal flaviviral infection of mosquitoes. | mosquitoes are natural vectors for many etiologic agents of human viral diseases. mosquito-borne flaviviruses can persistently infect the mosquito central nervous system without causing dramatic pathology or influencing the mosquito behavior and lifespan. the mechanism by which the mosquito nervous system resists flaviviral infection is still largely unknown. here we report that an aedes aegypti homologue of the neural factor hikaru genki (aahig) efficiently restricts flavivirus infection of the ... | 2015 | 25915054 |
| the hub protein loquacious connects the microrna and short interfering rna pathways in mosquitoes. | aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. rna interference (rnai) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. double-stranded rna binding proteins (dsrbps) are important for efficient rnai; in drosophila functional specialization of the mirna, endo-sirna and exo-sirna pathway is aided by the dsrbps loquacious (loqs-pb, loqs-pd) and r2d2, respectiv ... | 2015 | 25765650 |
| rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector. | millions of people are infected each year by arboviruses (arthropod-borne viruses) such as chikungunya, dengue, and west nile viruses, yet for reasons that are largely unknown, only a relatively small number of mosquito species are able to transmit arboviruses. understanding the complex factors that determine vector competence could facilitate strategies for controlling arbovirus infections. apoptosis is a potential antiviral defense response that has been shown to be important in other virus-ho ... | 2015 | 25713358 |
| suppressing dengue-2 infection by chemical inhibition of aedes aegypti host factors. | dengue virus host factors (denv hfs) that are essential for the completion of the infection cycle in the mosquito vector and vertebrate host represent potent targets for transmission blocking. here we investigated whether known mammalian denv hf inhibitors could influence virus infection in the arthropod vector a. aegypti. we evaluated the potency of bafilomycin (baf; inhibitor of vacuolar h+-atpase (vatpase)), mycophenolic acid (mpa; inhibitor of inosine-5'-monophosphate dehydrogenase (impdh)), ... | 2014 | 25101828 |
| complement-related proteins control the flavivirus infection of aedes aegypti by inducing antimicrobial peptides. | the complement system functions during the early phase of infection and directly mediates pathogen elimination. the recent identification of complement-like factors in arthropods indicates that this system shares common ancestry in vertebrates and invertebrates as an immune defense mechanism. thioester (te)-containing proteins (teps), which show high similarity to mammalian complement c3, are thought to play a key role in innate immunity in arthropods. herein, we report that a viral recognition ... | 2014 | 24722701 |
| microrna-like viral small rna from dengue virus 2 autoregulates its replication in mosquito cells. | micrornas (mirnas) are small regulatory rnas that play significant roles in most cellular processes. in the seemingly endless arms race between hosts and pathogens, viruses also encode mirnas that facilitate successful infection. in search of functional mirnas or viral small rnas (vsrnas) encoded by dengue virus (denv), deep sequencing data of virus-infected aedes aegypti mosquitoes were used. from six vsrnas, with candidate stem-loop structures in the 5' and 3' untranslated regions of the viral ... | 2014 | 24550303 |
| novel genetic and molecular tools for the investigation and control of dengue virus transmission by mosquitoes. | aedes aegypti is the principal vector of dengue virus (denv) throughout the tropical world. this anthropophilic mosquito species needs to be persistently infected with denv before it can transmit the virus through its saliva to a new vertebrate host. in the mosquito, denv is confronted with several innate immune pathways, among which rna interference is considered the most important. the ae. aegypti genome project opened the doors for advanced molecular studies on pathogen-vector interactions in ... | 0 | 24693489 |
| tracking dengue virus intra-host genetic diversity during human-to-mosquito transmission. | dengue virus (denv) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. this intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. to track changes in viral intra-host genetic diversity during horizontal transmission, we infected aedes aegypti mosquitoes by allowing them to feed on denv2-infected patients. we then p ... | 2015 | 26325059 |
| comparative susceptibility of mosquito populations in north queensland, australia to oral infection with dengue virus. | dengue is the most prevalent arthropod-borne virus, with at least 40% of the world's population at risk of infection each year. in australia, dengue is not endemic, but viremic travelers trigger outbreaks involving hundreds of cases. we compared the susceptibility of aedes aegypti mosquitoes from two geographically isolated populations to two strains of dengue virus serotype 2. we found, interestingly, that mosquitoes from a city with no history of dengue were more susceptible to virus than mosq ... | 2014 | 24420782 |
| dynamics of midgut microflora and dengue virus impact on life history traits in aedes aegypti. | significant morbidity and potential mortality following dengue virus infection is a re-emerging global health problem. due to the limited effectiveness of current disease control methods, mosquito biologists have been searching for new methods of controlling dengue transmission. while much effort has concentrated on determining genetic aspects to vector competence, paratransgenetic approaches could also uncover novel vector control strategies. the interactions of mosquito midgut microflora and p ... | 2014 | 25193134 |
| reemergence and autochthonous transmission of dengue virus, eastern china, 2014. | in 2014, 20 dengue cases were reported in the cities of wenzhou (5 cases) and wuhan (15 cases), china, where dengue has rarely been reported. dengue virus 1 was detected in 4 patients. although most of these cases were likely imported, epidemiologic analysis provided evidence for autochthonous transmission. | 0 | 26292098 |
| complex phenotypes in mosquitoes and mice associated with neutralization escape of a dengue virus type 1 monoclonal antibody. | denv1-e106 is a monoclonal antibody (mab) with strong neutralizing activity against all five denv-1 genotypes and therapeutic activity in mice. here, we evaluated the potential for denv-1 to escape neutralization by denv1-e106. a single mutation in domain iii of the envelope protein (t329a) emerged, which conferred resistance to denv1-e106. however, the t329a variant virus had differing phenotypes in vitro and in vivo with attenuation in cell culture yet increased infectivity in aedes aegypti mo ... | 2012 | 22406169 |
| cpb1 of aedes aegypti interacts with denv2 e protein and regulates intracellular viral accumulation and release from midgut cells. | aedes aegypti is a principal vector responsible for the transmission of dengue viruses (denv). to date, vector control remains the key option for dengue disease management. to develop new vector control strategies, a more comprehensive understanding of the biological interactions between denv and ae. aegypti is required. in this study, a cdna library derived from the midgut of female adult ae. aegypti was used in yeast two-hybrid (y2h) screenings against denv2 envelope (e) protein. among the man ... | 2014 | 25521592 |
| co-circulation of dengue and chikungunya viruses, al hudaydah, yemen, 2012. | we investigated 400 cases of dengue-like illness in persons hospitalized during an outbreak in al hudaydah, yemen, in 2012. overall, 116 dengue and 49 chikungunya cases were diagnosed. dengue virus type 2 was the predominant serotype. the co-circulation of these viruses indicates that mosquitoborne infections represent a public health threat in yemen. | 0 | 25061762 |
| dengue transmission model by means of viremic adult immuno-competent mouse. | dengue virus infection manifests in three distinct forms in humans: dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. infection with the virus is a fatal disease; no vaccine is available and prevention depends on interruption of the chain of transmission. the study of dengue viral transmission by mosquitoes is hindered due to the lack of an affordable animal model. in general, immuno-competent mice are used as a simple and inexpensive animal model, but mice are not susceptible t ... | 2014 | 24685121 |
| vertical transmission of dengue virus in aedes aegypti collected in puerto iguazú, misiones, argentina. | a finding of vertical transmission of the den 3 virus in male specimens of aedes aegypti, collected in the 2009 fall-winter period, in puerto iguazú city, misiones, argentina, using the rt-pcr technique in a 15-specimen pool is reported. this result is analyzed within the context of the epidemiological situation of argentina's northeast border. | 0 | 24626420 |
| the influence of dengue virus serotype-2 infection on aedes aegypti (diptera: culicidae) motivation and avidity to blood feed. | dengue virus (denv) is transmitted by aedes aegypti, a species that lives in close association with human dwellings. the behavior of denv-infected mosquitoes needs further investigation, especially regarding the potential influence of denv on mosquito biting motivation and avidity. | 2013 | 23755202 |
| differential expression of apoptosis related genes in selected strains of aedes aegypti with different susceptibilities to dengue virus. | aedes aegypti is the principal vector of dengue viruses worldwide. we identified field collected insects with differential susceptibility to dengue-2 virus (denv-2) and used isofemale selection to establish susceptible and refractory strains based on midgut infection barriers. previous experiments had identified higher expression of apoptosis-related genes in the refractory strain. to identify potential molecular mechanisms associated with denv susceptibility, we evaluated the differential expre ... | 2013 | 23593426 |
| age-dependent effects of oral infection with dengue virus on aedes aegypti (diptera: culicidae) feeding behavior, survival, oviposition success and fecundity. | aedes aegypti is the main vector of dengue, a disease that is increasing its geographical range as well as incidence rates. despite its public health importance, the effect of dengue virus (denv) on some mosquito traits remains unknown. here, we investigated the impact of denv-2 infection on the feeding behavior, survival, oviposition success and fecundity of ae. aegypti females. | 2013 | 23555838 |
| mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. | dengue virus (denv), a flavivirus of global importance, is transmitted to humans by mosquitoes. in this study, we developed in vitro and in vivo models of saliva-mediated enhancement of denv infectivity. serine protease activity in aedes aegypti saliva augmented virus infectivity in vitro by proteolyzing extracellular matrix proteins, thereby increasing viral attachment to heparan sulfate proteoglycans and inducing cell migration. a serine protease inhibitor reduced saliva-mediated enhancement o ... | 2013 | 24131723 |
| genetic variation in the 3' untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in brazil. | dengue, a mosquito-borne viral infection caused by one of the four dengue virus (denv) serotypes (denv-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections throughout tropical and subtropical regions of the world. in brazil, the disease has become a major public health problem and the introduction of denv-3 in 2000 in rio de janeiro (rj) was associated with severe dengue epidemics. the potential emergence of strains associated with severe diseas ... | 2013 | 23282086 |
| physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households. | primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ... | 2016 | 27164168 |
| zika virus: indian perspectives. | the emergence of zika virus (ziv), a mosquito borne flavivirus like dengue (den) and chikungunya (chik), in brazil in 2014 and its spread to various countries have led to a global health emergency. aedes aegypti is the major vector for ziv. fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ziv and there is abundance of aedes mosquitoes. in this review, we focus on current global scenar ... | 0 | 27487998 |
| zika virus infections imported from brazil to portugal, 2015. | zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ... | 2016 | 27134823 |
| surveillance for dengue and dengue-associated neurologic syndromes in the united states. | autochthonous dengue virus transmission has occurred in the continental united states with increased frequency during the last decade; the principal vector, aedes aegypti, has expanded its geographic distribution in the southern united states. dengue, a potentially fatal arboviral disease, is underreported, and us clinicians encountering patients with acute febrile illness consistent with dengue are likely to not be fully familiar with dengue diagnosis and management. recently, investigators sug ... | 2014 | 25371183 |
| comparative susceptibility of aedes albopictus and aedes aegypti to dengue virus infection after feeding on blood of viremic humans: implications for public health. | aedes albopictus is secondary to aedes aegypti as a vector of dengue viruses (denvs) in settings of endemicity, but it plays an important role in areas of dengue emergence. this study compared the susceptibility of these 2 species to denv infection by performing 232 direct blood-feeding experiments on 118 viremic patients with dengue in vietnam. field-derived a. albopictus acquired denv infections as readily as a. aegypti after blood feeding. once infected, a. albopictus permitted higher concent ... | 2015 | 25784733 |
| naturally-acquired dengue virus infections do not reduce short-term survival of infected aedes aegypti from ho chi minh city, vietnam. | transmission of dengue virus (denv) from mosquito to human is dependent upon the survival of the mosquito beyond the virus extrinsic incubation period. previous studies report conflicting results of the effects of denv on aedes aegypti survival. here, we describe the effect of denv on the short-term survival (up to 12 d) of 4,321 ae. aegypti mosquitoes blood-fed on 150 ns1-positive dengue patients hospitalized in the hospital for tropical diseases, ho chi minh city, vietnam. mosquito survival wa ... | 2015 | 25561566 |
| households as foci for dengue transmission in highly urban vietnam. | dengue control programs commonly employ reactive insecticide spraying around houses of reported cases, with the assumption that most dengue virus (denv) transmission occurs in the home. focal household transmission has been demonstrated in rural settings, but it is unclear whether this holds true in dense and mobile urban populations. we conducted a prospective study of dengue clustering around households in highly urban ho chi minh city, vietnam. | 2015 | 25680106 |
| a database of circadian and diel rhythmic gene expression in the yellow fever mosquito aedes aegypti. | the mosquito species aedes aegypti is the primary vector of many arboviral diseases, including dengue and yellow fevers, that are responsible for a large worldwide health burden. the biological rhythms of mosquitoes regulate many of the physiological processes and behaviors that influence the transmission of these diseases. for insight into the molecular basis of biological rhythms, diel and circadian gene expression profiling has been carried out for many species. to bring these resources to ae ... | 2014 | 25516260 |
| mosquito larvicidal and antimicrobial activity of protein of solanum villosum leaves. | mosquitoes are associated with the transmission of malaria, dengue, japanese encephalitis, filariasis and other viral diseases throughout the globe, apart from being a nuisance pest. biological control alone or as a part of integrated vector management stands to be a better alternative to the chemical controls aimed against pest mosquitoes. at the same time it is necessary to control bacteria by synthetic or natural means (plant products). hence the present study was designed to screen the effec ... | 2008 | 19061512 |
| occurrence of natural vertical transmission of dengue-2 and dengue-3 viruses in aedes aegypti and aedes albopictus in fortaleza, ceará, brazil. | aedes aegypti and aedes albopictus perform an important role in the transmission of the dengue virus to human populations, particularly in the tropical and subtropical regions of the world. despite a lack of understanding in relation to the maintenance of the dengue virus in nature during interepidemic periods, the vertical transmission of the dengue virus in populations of a. aegypti and a. albopictus appears to be of significance in relation to the urban scenario of fortaleza. | 2012 | 22848479 |
| fine scale spatiotemporal clustering of dengue virus transmission in children and aedes aegypti in rural thai villages. | based on spatiotemporal clustering of human dengue virus (denv) infections, transmission is thought to occur at fine spatiotemporal scales by horizontal transfer of virus between humans and mosquito vectors. to define the dimensions of local transmission and quantify the factors that support it, we examined relationships between infected humans and aedes aegypti in thai villages. | 2012 | 22816001 |
| species composition, seasonal occurrence, habitat preference and altitudinal distribution of malaria and other disease vectors in eastern nepal. | it is increasingly recognized that climate change can alter the geographical distribution of vector-borne diseases (vbds) with shifts of disease vectors to higher altitudes and latitudes. in particular, an increasing risk of malaria and dengue fever epidemics in tropical highlands and temperate regions has been predicted in different climate change scenarios. the aim of this paper is to expand the current knowledge on the seasonal occurrence and altitudinal distribution of malaria and other dise ... | 2014 | 25430654 |
| the burden of dengue and chikungunya worldwide: implications for the southern united states and california. | dengue virus (denv) spreads to humans through the bite of an infected aedes aegypti or aedes albopictus mosquito and is a growing public health threat to both industrialized and developing nations worldwide. outbreaks of autochthonous dengue in the united states occurred extensively in the past but over the past 3 decades have again taken place in florida, hawaii, and texas as well as in american samoa, guam, northern mariana islands, puerto rico, and the us virgin islands. as the aedes vectors ... | 0 | 25960096 |
| increased replicative fitness of a dengue virus 2 clade in native mosquitoes: potential contribution to a clade replacement event in nicaragua. | the four dengue virus (denv) serotypes (denv serotype 1 [denv-1] to denv-4) are transmitted by aedes aegypti and a. albopictus mosquitoes, causing up to 390 million denv infections worldwide each year. we previously reported a clade replacement of the denv-2 asian-american genotype ni-1 clade by the ni-2b clade in managua, nicaragua. here, we describe our studies of the replicative ability of ni-1 and ni-2b viruses in an a. aegypti cell line (aag2) and a. aegypti mosquitoes reared from eggs coll ... | 2014 | 25187539 |
| dengue virus infections among haitian and expatriate non-governmental organization workers--léogane and port-au-prince, haiti, 2012. | in october 2012, the haitian ministry of health and the us cdc were notified of 25 recent dengue cases, confirmed by rapid diagnostic tests (rdts), among non-governmental organization (ngo) workers. we conducted a serosurvey among ngo workers in léogane and port-au-prince to determine the extent of and risk factors for dengue virus infection. of the total 776 staff from targeted ngos in léogane and port-au-prince, 173 (22%; 52 expatriates and 121 haitians) participated. anti-dengue virus (denv) ... | 2014 | 25356592 |
| genetic and phenotypic characterization of manufacturing seeds for a tetravalent dengue vaccine (denvax). | we have developed a manufacturing strategy that can improve the safety and genetic stability of recombinant live-attenuated chimeric dengue vaccine (denvax) viruses. these viruses, containing the pre-membrane (prm) and envelope (e) genes of dengue serotypes 1-4 in the replicative background of the attenuated dengue-2 pdk-53 vaccine virus candidate, were manufactured under cgmp. | 2013 | 23738026 |
| host and viral features of human dengue cases shape the population of infected and infectious aedes aegypti mosquitoes. | dengue is the most prevalent arboviral disease of humans. the host and virus variables associated with dengue virus (denv) transmission from symptomatic dengue cases (n = 208) to aedes aegypti mosquitoes during 407 independent exposure events was defined. the 50% mosquito infectious dose for each of denv-1-4 ranged from 6.29 to 7.52 log10 rna copies/ml of plasma. increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of denv trans ... | 2013 | 23674683 |
| extensive circadian and light regulation of the transcriptome in the malaria mosquito anopheles gambiae. | mosquitoes exhibit 24 hr rhythms in flight activity, feeding, reproduction and development. to better understand the molecular basis for these rhythms in the nocturnal malaria vector anopheles gambiae, we have utilized microarray analysis on time-of-day specific collections of mosquitoes over 48 hr to explore the coregulation of gene expression rhythms by the circadian clock and light, and compare these with the 24 hr rhythmic gene expression in the diurnal aedes aegypti dengue vector mosquito. | 2013 | 23552056 |
| detection of dengue virus ns1 antigen in infected aedes aegypti using a commercially available kit. | epidemic dengue has emerged throughout the tropical world. in the continued absence of a vaccine against dengue virus (denv), mosquito vector surveillance and control programs are essential to reduce human infections. an effective test to detect denv in infected mosquitoes would be a valuable addition to the surveillance effort. we investigated denv detection in infected aedes aegypti using a commercially available denv non-structural protein 1 (ns1) elisa kit (platelia dengue ns1 ag), and by re ... | 2012 | 23185074 |
| identification of new protein interactions between dengue fever virus and its hosts, human and mosquito. | the four divergent serotypes of dengue virus are the causative agents of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. about two-fifths of the world's population live in areas where dengue is prevalent, and thousands of deaths are caused by the viruses every year. dengue virus is transmitted from one person to another primarily by the yellow fever mosquito, aedes aegypti. recent studies have begun to define how the dengue viral proteins interact with host proteins to mediate ... | 2013 | 23326450 |
| first isolation of dengue virus from the 2010 epidemic in nepal. | dengue is an emerging disease in nepal and was first observed as an outbreak in nine lowland districts in 2006. in 2010, however, a large epidemic of dengue occurred with 4,529 suspected and 917 serologically-confirmed cases and five deaths reported in government hospitals in nepal. the collection of demographic information was performed along with an entomological survey and clinical evaluation of the patients. a total of 280 serum samples were collected from suspected dengue patients. these sa ... | 2013 | 24155651 |
| development of guidelines for the surveillance of invasive mosquitoes in europe. | the recent notifications of autochthonous cases of dengue and chikungunya in europe prove that the region is vulnerable to these diseases in areas where known mosquito vectors (aedes albopictus and aedes aegypti) are present. strengthening surveillance of these species as well as other invasive container-breeding aedine mosquito species such as aedes atropalpus, aedes japonicus, aedes koreicus and aedes triseriatus is therefore required. in order to support and harmonize surveillance activities ... | 2013 | 23866915 |
| asymptomatic humans transmit dengue virus to mosquitoes. | three-quarters of the estimated 390 million dengue virus (denv) infections each year are clinically inapparent. people with inapparent dengue virus infections are generally considered dead-end hosts for transmission because they do not reach sufficiently high viremia levels to infect mosquitoes. here, we show that, despite their lower average level of viremia, asymptomatic people can be infectious to mosquitoes. moreover, at a given level of viremia, denv-infected people with no detectable sympt ... | 2015 | 26553981 |
| acetylcholinesterases from the disease vectors aedes aegypti and anopheles gambiae: functional characterization and comparisons with vertebrate orthologues. | mosquitoes of the anopheles (an.) and aedes (ae.) genus are principal vectors of human diseases including malaria, dengue and yellow fever. insecticide-based vector control is an established and important way of preventing transmission of such infections. currently used insecticides can efficiently control mosquito populations, but there are growing concerns about emerging resistance, off-target toxicity and their ability to alter ecosystems. a potential target for the development of insecticide ... | 2015 | 26447952 |
| mosquito larvicidal activities of solanum villosum berry extract against the dengue vector stegomyia aegypti. | vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. although several plants have been reported for mosquitocidal activity, only a few botanicals have moved from the laboratory to field use, because they are poorly characterized, in most cases active principals are not determined and most of the works are restricted to preliminary screening. solanum v ... | 2008 | 18387176 |
| mosquito vector diversity across habitats in central thailand endemic for dengue and other arthropod-borne diseases. | recent years have seen the greatest ecological disturbances of our times, with global human expansion, species and habitat loss, climate change, and the emergence of new and previously-known infectious diseases. biodiversity loss affects infectious disease risk by disrupting normal relationships between hosts and pathogens. mosquito-borne pathogens respond to changing dynamics on multiple transmission levels and appear to increase in disturbed systems, yet current knowledge of mosquito diversity ... | 2013 | 24205420 |
| the potential use of wolbachia-based mosquito biocontrol strategies for japanese encephalitis. | japanese encephalitis virus (jev) is a zoonotic pathogen transmitted by the infectious bite of culex mosquitoes. the virus causes the development of the disease japanese encephalitis (je) in a small proportion of those infected, predominantly affecting children in eastern and southern asia. annual je incidence estimates range from 50,000-175,000, with 25%-30% of cases resulting in mortality. it is estimated that 3 billion people live in countries in which jev is endemic. the virus exists in an e ... | 2015 | 26086337 |
| effect of wolbachia on replication of west nile virus in a mosquito cell line and adult mosquitoes. | wolbachia as an endosymbiont is widespread in insects and other arthropods and is best known for reproductive manipulations of the host. recently, it has been shown that wmelpop and wmel strains of wolbachia inhibit the replication of several rna viruses, including dengue virus, and other vector-borne pathogens (e.g., plasmodium and filarial nematodes) in mosquitoes, providing an alternative approach to limit the transmission of vector-borne pathogens. in this study, we tested the effect of wolb ... | 2012 | 23115298 |
| salinity-tolerant larvae of mosquito vectors in the tropical coast of jaffna, sri lanka and the effect of salinity on the toxicity of bacillus thuringiensis to aedes aegypti larvae. | dengue, chikungunya, malaria, filariasis and japanese encephalitis are common mosquito-borne diseases endemic to sri lanka. aedes aegypti and aedes albopictus, the major vectors of dengue, were recently shown to undergo pre-imaginal development in brackish water bodies in the island. a limited survey of selected coastal localities of the jaffna district in northern sri lanka was carried out to identify mosquito species undergoing pre-imaginal development in brackish and saline waters. the effect ... | 2012 | 23174003 |
| preliminary evaluation on the efficiency of the kit platelia dengue ns1 ag-elisa to detect dengue virus in dried aedes aegypti: a potential tool to improve dengue surveillance. | surveillance is a critical component of any dengue prevention and control programme. herein, we investigate the efficiency of the commercial kit platelia dengue ns1 ag-elisa to detect dengue virus (denv) antigens in aedes aegypti mosquitoes infected under laboratory conditions. | 2014 | 24690324 |
| lethal effects of aspergillus niger against mosquitoes vector of filaria, malaria, and dengue: a liquid mycoadulticide. | aspergillus niger is a fungus of the genus aspergillus. it has caused a disease called black mold on certain fruits and vegetables. the culture filtrates released from the a. niger atcc 66566 were grown in czapek dox broth (cdb) then filtered with flash chromatograph and were used for the bioassay after a growth of thirty days. the result demonstrated these mortalities with lc(50), lc(90), and lc(99) values of culex quinquefasciatus 0.76, 3.06, and 4.75, anopheles stephensi 1.43, 3.2, and 3.86, ... | 2012 | 22629156 |
| evaluation of 15 local plant species as larvicidal agents against an indian strain of dengue fever mosquito, aedes aegypti l. (diptera: culicidae). | the adverse effects of chemical insecticides-based intervention measures for the control of mosquito vectors have received wide public apprehension because of several problems like insecticide resistance, resurgence of pest species, environmental pollution, toxic hazards to humans, and non-target organisms. these problems have necessitated the need to explore and develop alternative strategies using eco-friendly, environmentally safe, bio-degradable plant products which are non-toxic to non-targ ... | 2012 | 22536188 |
| challenges in estimating insecticide selection pressures from mosquito field data. | insecticide resistance has the potential to compromise the enormous effort put into the control of dengue and malaria vector populations. it is therefore important to quantify the amount of selection acting on resistance alleles, their contributions to fitness in heterozygotes (dominance) and their initial frequencies, as a means to predict the rate of spread of resistance in natural populations. we investigate practical problems of obtaining such estimates, with particular emphasis on mexican p ... | 2011 | 22069506 |
| the asian tiger hunts in maputo city--the first confirmed report of aedes (stegomyia) albopictus (skuse, 1895) in mozambique. | increasing evidence suggests that dengue fever is endemic in mozambique. larvae of both the afrotropical vector aedes aegypti and its subspecies, ae. aegypti formosus, have been reported from three provinces in mozambique, two of which recently experienced dengue outbreaks. despite reports of the invasive oriental vector ae. albopictus on the islands in the mozambique channel and nearby indian ocean, the species has not yet been reported in mozambique. | 2016 | 26856329 |
| behavioral response of aedes aegypti mosquito towards essential oils using olfactometer. | aedes aegypti mosquito is responsible for transmitting human diseases like dengue and chikungunya. personal or space protection with insect repellents is a practical approach to reducing human mosquito contact, thereby minimizing disease transmission. essential oils are natural volatile substances from plants used as protective measure against blood-sucking mosquitoes. | 2016 | 27308295 |
| role of aedes aegypti (linnaeus) and aedes albopictus (skuse) in local dengue epidemics in taiwan. | aedes mosquitoes in taiwan mainly comprise aedes albopictus and ae. aegypti. however, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of ae. albopictus and ae. aegypti. | 2016 | 27829399 |
| vertical transmission of zika virus in aedes aegypti mosquitoes. | previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ... | 2016 | 27573623 |
| the golgi associated eri3 is a flavivirus host factor. | dengue virus (denv) is a mosquito-borne flavivirus classified into four serotypes (denv-1-4) that causes dengue fever (df), dengue hemorrhagic fever (dhf) or dengue shock syndrome (dss). an estimated 390 million people are at risk for infection with denv and there are no effective vaccines or therapeutics. we utilized rna chromatography coupled with quantitative mass spectrometry (qms) to identify host rna binding proteins (rbps) that interact with denv-2 rna. we identified eri3 (also prnpip and ... | 2016 | 27682269 |
| new paradigms for virus detection, surveillance and control of zika virus vectors in the settings of southeast asia. | zika virus (zikv) has now become a global public health concern. the vectors for zikv are aedes aegypti and a. albopictus. both these mosquitoes are predominant in southeast asia and are also responsible for the spread of other arboviral diseases like dengue virus and chikungunya virus. the incidence of dengue has been increasing over the years and this is of concern to public health workers. simple laboratory tools for the detection of zikv is also lacking. in the absence of drugs and vaccine f ... | 2016 | 27679623 |
| larval application of sodium channel homologous dsrna restores pyrethroid insecticide susceptibility in a resistant adult mosquito population. | mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. the emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and zika. therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. herein we demonstrated the use of rna interference ... | 2016 | 27416771 |
| effect of dengue-2 virus infection on protein expression in the salivary glands of aedes aegypti mosquitoes. | dengue virus (denv) is the most important mosquito-transmitted flavivirus that is transmitted throughout the tropical and subtropical regions of the world. the primary mosquito vector of denv in urban locations is aedes aegypti. key to understanding the transmission of denv is the relationship between pathogen and vector. accordingly, we report our preliminary characterization of the differentially expressed proteins from ae. aegypti mosquitoes after denv infection. we investigated the virus-vec ... | 2014 | 24445208 |
| vectorial capacity of aedes aegypti: effects of temperature and implications for global dengue epidemic potential. | dengue is a mosquito-borne viral disease that occurs mainly in the tropics and subtropics but has a high potential to spread to new areas. dengue infections are climate sensitive, so it is important to better understand how changing climate factors affect the potential for geographic spread and future dengue epidemics. vectorial capacity (vc) describes a vector's propensity to transmit dengue taking into account human, virus, and vector interactions. vc is highly temperature dependent, but most ... | 2014 | 24603439 |
| reduction of aedes aegypti vector competence for dengue virus under large temperature fluctuations. | diurnal temperature fluctuations can fundamentally alter mosquito biology and mosquito-virus interactions in ways that impact pathogen transmission. we investigated the effect of two daily fluctuating temperature profiles on aedes aegypti vector competence for dengue virus (denv) serotype-1. a large diurnal temperature range of 18.6°c around a 26°c mean, corresponding with the low denv transmission season in northwestern thailand, reduced midgut infection rates and tended to extend the virus ext ... | 2013 | 23438766 |
| mayaro virus and dengue virus 1 and 4 natural infection in culicids from cuiabá, state of mato grosso, brazil. | this study aimed to verify the diversity of culicidae species and their frequency of infection with flaviviruses and alphaviruses in cuiabá, state of mato grosso, brazil. mosquitoes were captured with nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (rt-pcr) for 11 flavivirus and five alphavirus. p ... | 0 | 26784852 |
| clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil. | dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously. | 2014 | 24978469 |
| is there a risk of yellow fever virus transmission in south asian countries with hyperendemic dengue? | the fact that yellow fever (yf) has never occurred in asia remains an "unsolved mystery" in global health. most countries in asia with high aedes aegypti mosquito density are considered "receptive" for yf transmission. recently, health officials in sri lanka issued a public health alert on the potential spread of yf from a migrant group from west africa. we performed an extensive review of literature pertaining to the risk of yf in sri lanka/south asian region to understand the probability of ac ... | 2013 | 24367789 |
| a draft genome sequence of an invasive mosquito: an italian aedes albopictus. | the draft genome sequence of italian specimens of the asian tiger mosquito aedes (stegomyia) albopictus (diptera: culicidae) was determined using a standard ngs (next generation sequencing) approach. the size of the assembled genome is comparable to that of aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive dna is concerned, most of which is made up of transposable elements. although, based on busco (benchmarking universal single-copy orthologues) analysi ... | 2015 | 26369436 |
| wolbachia reduces the transmission potential of dengue-infected aedes aegypti. | dengue viruses (denv) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. the wolbachia strain wmel, which has been introduced into the mosquito vector, aedes aegypti, has been shown to invade and spread to near fix ... | 2015 | 26115104 |
| aedes aegypti control strategies in brazil: incorporation of new technologies to overcome the persistence of dengue epidemics. | dengue is considered to be the most important mosquito-borne viral disease in the world. the aedes aegypti mosquito, its vector, is highly anthropophilic and is very well adapted to urban environments. although several vaccine candidates are in advanced stages of development no licensed dengue vaccine is yet available. as a result, controlling the spread of dengue still requires that mosquitoes be targeted directly. we review the current methods of dengue vector control focusing on recent techni ... | 2015 | 26463204 |
| high-level gene expression in aedes albopictus cells using a baculovirus hr3 enhancer and ie1 transactivator. | aedes aegypti is the key vector of both the yellow fever and dengue fever viruses throughout many parts of the world. low and variable transgene expression levels due to position effect and position effect variegation are problematic to efforts to create transgenic laboratory strains refractory to these viruses. transformation efficiencies are also less than optimal, likely due to failure to detect expression from all integrated transgenes and potentially due to limited expression of the transpo ... | 2004 | 15251037 |