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rhamnolipids: solution against aedes aegypti?aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever, and chikungunya viruses. this mosquito has developed resistance to the insecticides currently used to control their populations. these chemical insecticides are harmful to the environment and can have negative effects on human health. rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. the present study evaluated the potentia ...201525762986
the hub protein loquacious connects the microrna and short interfering rna pathways in mosquitoes.aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. rna interference (rnai) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. double-stranded rna binding proteins (dsrbps) are important for efficient rnai; in drosophila functional specialization of the mirna, endo-sirna and exo-sirna pathway is aided by the dsrbps loquacious (loqs-pb, loqs-pd) and r2d2, respectiv ...201525765650
susceptibility of indigenous and transplanted mosquito spp. to dengue virus in japan.dengue fever, an acute, mosquito-borne, febrile illness caused by flavivirus spp., is a problem in africa, south and southeast asia, latin america, and the caribbean. a dengue outbreak occurred after nearly 70 years of absence or no detection, and then 158 autochthonous cases occurred in japan from august to october 15, 2014. the most competent mosquito vectors for dengue virus transmission were aedes aegypti and a. albopictus. since a. albopictus is widely distributed across japan and a. aegypt ...201525766611
angelica sinensis (umbelliferae) with proven repellent properties against aedes aegypti, the primary dengue fever vector in thailand.botanical resources with great diversity in medicinal and aromatic plants are a rich and reliable source for finding insect repellents of plant origin, which are widely popular among today's consumers. although some herbal-based repellents have been proven comparable to or even better than synthetics, commercially available natural repellents generally tend to be expensive, with short-lived effectiveness. this critical flaw leads to ongoing research for new and effective repellents, which provid ...201525773182
risk factors for the presence of chikungunya and dengue vectors (aedes aegypti and aedes albopictus), their altitudinal distribution and climatic determinants of their abundance in central nepal.the presence of the recently introduced primary dengue virus vector mosquito aedes aegypti in nepal, in association with the likely indigenous secondary vector aedes albopictus, raises public health concerns. chikungunya fever cases have also been reported in nepal, and the virus causing this disease is also transmitted by these mosquito species. here we report the results of a study on the risk factors for the presence of chikungunya and dengue virus vectors, their elevational ceiling of distri ...201525774518
silencing of end-joining repair for efficient site-specific gene insertion after talen/crispr mutagenesis in aedes aegypti.conventional control strategies for mosquito-borne pathogens such as malaria and dengue are now being complemented by the development of transgenic mosquito strains reprogrammed to generate beneficial phenotypes such as conditional sterility or pathogen resistance. the widespread success of site-specific nucleases such as transcription activator-like effector nucleases (talens) and clustered regularly interspaced short palindromic repeats (crispr)/cas9 in model organisms also suggests that repro ...201525775608
possible association between recent migration and hospitalisation for dengue in an urban population: a prospective case-control study in northern vietnam.a prospective case-control study was conducted in urban districts in hanoi, northern vietnam to evaluate the effect of migration on the risk of hospitalisation for dengue in a vietnamese urban population. we enrolled laboratory-confirmed dengue patients aged ≥ 18 years who were hospitalised in local hospitals in november and december 2010. four neighbourhood-matched controls for each case were recruited within a week of hospitalisation. sociodemographic data were collected by interviews, and the ...201425776595
comparative susceptibility of aedes albopictus and aedes aegypti to dengue virus infection after feeding on blood of viremic humans: implications for public health.aedes albopictus is secondary to aedes aegypti as a vector of dengue viruses (denvs) in settings of endemicity, but it plays an important role in areas of dengue emergence. this study compared the susceptibility of these 2 species to denv infection by performing 232 direct blood-feeding experiments on 118 viremic patients with dengue in vietnam. field-derived a. albopictus acquired denv infections as readily as a. aegypti after blood feeding. once infected, a. albopictus permitted higher concent ...201525784733
modeling the impact on virus transmission of wolbachia-mediated blocking of dengue virus infection of aedes aegypti.dengue is the most common arboviral infection of humans and is a public health burden in more than 100 countries. aedes aegypti mosquitoes stably infected with strains of the intracellular bacterium wolbachia are resistant to dengue virus (denv) infection and are being tested in field trials. to mimic field conditions, we experimentally assessed the vector competence of a. aegypti carrying the wolbachia strains wmel and wmelpop after challenge with viremic blood from dengue patients. we found th ...025787763
effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti.currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ...201525796215
larvicidal activity of few select indigenous plants of north east india against disease vector mosquitoes (diptera: culicidae).mosquitoes are the vectors of several life threatening diseases like dengue, malaria, japanese encephalitis and lymphatic filariasis, which are widely present in the north-eastern states of india. investigations on five local plants of north-east india, selected on the basis of their use by indigenous communities as fish poison, were carried out to study their mosquito larvicidal potential against anopheles stephensi (malaria vector), stegomyia aegypti (dengue vector) and culex quinquefasciatus ...201525801251
surveillance of aedes albopictus skuse breeding preference in selected dengue outbreak localities, peninsular malaysia.entomological surveillance was conducted in order to determine the abundance and to evaluate any changes of biological vectors or ecology, especially in the dengue outbreak areas. the abundance and breeding preference of aedes albopictus and aedes aegypti were conducted in selected dengue outbreak localities in three states of peninsular malaysia namely selangor, federal territory of kuala lumpur, and penang island using ovitraps and larval survey method. it was determined that ae. albopictus wa ...201525801254
evaluation of sumithion l-40 against aedes aegypti (l.) and aedes albopictus skuse.space spraying of chemical insecticides is still an important mean of controlling aedes mosquitoes and dengue transmission. for this purpose, the bioefficacy of space-sprayed chemical insecticide should be evaluated from time to time. a simulation field trial was conducted outdoor in an open field and indoor in unoccupied flat units in kuala lumpur, to evaluate the adulticidal and larvicidal effects of sumithion l-40, a ulv formulation of fenitrothion. a thermal fogger with a discharge rate of 2 ...201525801256
low efficacy of delthamethrin-treated net against singapore aedes aegypti is associated with kdr-type resistance.there has been a worldwide surge in the number and severity of dengue in the past decades. in singapore, relentless vector control efforts have been put in to control the disease since the 1960's. space spraying, fogging, chemical treatment and source reduction are some commonly used methodologies for controlling its vectors, particularly aedes aegypti. here, as we explored the use of a commercially available delthamethrin-treated net as an alternative strategy and the efficacy of the treated ne ...201525801264
the influence of diet on the use of near-infrared spectroscopy to determine the age of female aedes aegypti mosquitoes.interventions targeting adult mosquitoes are used to combat transmission of vector-borne diseases, including dengue. without available vaccines, targeting the primary vector, aedes aegypti, is essential to prevent transmission. older mosquitoes (≥ 7 days) are of greatest epidemiological significance due to the 7-day extrinsic incubation period of the virus. age-grading of female mosquitoes is necessary to identify post-intervention changes in mosquito population age structure. we developed model ...201525802436
mitochondrial physiology in the major arbovirus vector aedes aegypti: substrate preferences and sexual differences define respiratory capacity and superoxide production.adult females of aedes aegypti are facultative blood sucking insects and vectors of dengue and yellow fever viruses. insect dispersal plays a central role in disease transmission and the extremely high energy demand posed by flight is accomplished by a very efficient oxidative phosphorylation process, which take place within flight muscle mitochondria. these organelles play a central role in energy metabolism, interconnecting nutrient oxidation to atp synthesis, but also represent an important s ...201525803027
an entomological review of invasive mosquitoes in europe.among the invasive mosquitoes registered all over the world, aedes species are particularly frequent and important. as several of them are potential vectors of disease, they present significant health concerns for 21st century europe. five species have established in mainland europe, with two (aedes albopictus and aedes japonicus) becoming widespread and two (ae. albopictus and aedes aegypti) implicated in disease transmission to humans in europe. the routes of importation and spread are often e ...201525804287
the rising importance of triatoma rubrofasciata.the migration of invasive vector species has contributed to the worldwide extension of infectious diseases such as dengue (aedes aegypti) and chikungunya (aedes albopictus). it is probably a similar behaviour for certain vectors of chagas disease which allowed it to become a continental burden in latin america. one of them, triatoma rubrofasciata has also been spreading throughout the tropical and subtropical world. here, the recent and massive peridomestic presence of t. rubrofasciata in vietna ...201525807471
exome and transcriptome sequencing of aedes aegypti identifies a locus that confers resistance to brugia malayi and alters the immune response.many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode brugia malayi. the genome of ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. we designed exome captur ...201525815506
reappearance of chikungunya, formerly called dengue, in the americas.after an absence of ≈200 years, chikungunya returned to the american tropics in 2013. the virus is maintained in a complex african zoonotic cycle but escapes into an urban cycle at 40- to 50-year intervals, causing global pandemics. in 1823, classical chikungunya, a viral exanthem in humans, occurred on zanzibar, and in 1827, it arrived in the caribbean and spread to north and south america. in zanzibar, the disease was known as kidenga pepo, swahili for a sudden cramp-like seizure caused by an ...025816211
genome engineering with crispr-cas9 in the mosquito aedes aegypti.the mosquito aedes aegypti is a potent vector of the chikungunya, yellow fever, and dengue viruses, responsible for hundreds of millions of infections and over 50,000 human deaths per year. mutagenesis in ae. aegypti has been established with talens, zfns, and homing endonucleases, which require the engineering of dna-binding protein domains to provide genomic target sequence specificity. here, we describe the use of the crispr-cas9 system to generate site-specific mutations in ae. aegypti. this ...201525818303
cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod mesocyclops aspericornis against malaria and dengue mosquitoes.plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. however, how they affect the activity of mosquito predators in the aquatic environment is unknown. in this study, we synthesized gold nanoparticles (aun) using the leaf extract of cymbopogon citratus, which acted as a reducing and capping agent. aun were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. c. citratus extract ...201525819295
mosquito vector biology and control in latin america-a 24th symposium.the 24th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 80th annual meeting in seattle, wa, in february 2014. the principal objective, for the previous 23 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 26 presentations that were given orally in spanish or presented as posters by participants fr ...201425843096
comparison of bg-sentinel® trap and oviposition cups for aedes aegypti and aedes albopictus surveillance in jacksonville, florida, usa.the bg-sentinel® (bgs) trap and oviposition cups (ocs) have both proven effective in the surveillance of aedes species. this study aimed to determine which of the 2 traps could best characterize the relative population sizes of aedes albopictus and aedes aegypti in an urban section of jacksonville, fl. until 1986, ae. aegypti was considered the dominant container-breeding species in urban northeastern florida. since the introduction of ae. albopictus, ae. aegypti has become almost completely ext ...201525843173
evaluation of household bleach as an ovicide for the control of aedes aegypti.accumulations of dormant eggs in container habitats allow aedes aegypti populations to survive harsh environmental conditions and may frustrate control interventions directed at larval and adult life stages. while sodium hypochlorite solutions (naocl) have long been recognized as ovicides for use against dengue vectors, the susceptibility of eggs to spray applications has not been robustly evaluated on substrate materials representative of the most frequently utilized artificial container habita ...025843179
evaluation of the atraedes™ lure for collection of culex quinquefasciatus in gravid traps.the typical attractant used in gravid trapping of culex quinquefasciatus is an aged infusion of organic materials, which can change in attractiveness over time. a standardized chemical attractant dispenser derived from grass infusion, the atraedes™ lure, has been produced for the surveillance of the dengue vector aedes aegypti. a study using this lure in combination with the centers for disease control and prevention gravid traps was conducted in tanga, tanzania. the addition of the lure to trap ...201525843185
imported cases of dengue in poland and their diagnosis.infections with dengue virus are transmitted by mosquitoes. in tropical areas, it is mainly spread by aedes aegypti while in countries with lower temperatures by aedes albopictus. since 2010, autochthonous cases of dengue are also reported in europe. there are 4 serotypes of dengue virus (denv). no correlation between clinical presentation of disease and virus type, however, were determined. nevertheless, reinfection with different type of denv may lead to a serious, life-threatening condition. ...201425848785
gc-ms analysis of bioactive components and synthesis of gold nanoparticle using chloroxylon swietenia dc leaf extract and its larvicidal activity.a rapid bio-reduction of chloroauric acid (haucl4) was achieved by chloroxylon swietenia dc leaf extract (csle), which resulted in the formation of well dispersed c. swietenia gold nanoparticles (csgnps). the formation of gnps was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. the characterization of synthesized csgnps was made through x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), high resolution transmission electron ...201525854160
[detection of flavivirus in mosquitoes (diptera: culicidae) from easter island-chile].flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera aedes and culex (culicidae) that are detected in tropical and subtropical areas. main flaviviruses of public health importance are: dengue, west nile virus, yellow fever, among others. in continental chile, flaviviruses has not been detected. however, there are indigenous cases of dengue detected in easter island since 2002, as the presence of its vector aedes aegypti. the aim of this study was: to determine diversity o ...201525860055
river boats contribute to the regional spread of the dengue vector aedes aegypti in the peruvian amazon.the dramatic range expansion of the dengue vector aedes aegypti is associated with various anthropogenic transport activities, but little is known about the underlying mechanisms driving this geographic expansion. we longitudinally characterized infestation of different vehicle types (cars, boats, etc.) to estimate the frequency and intensity of mosquito introductions into novel locations (propagule pressure).201525860352
natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil.dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce.201525860459
chitosan/interfering rna nanoparticle mediated gene silencing in disease vector mosquito larvae.vector mosquitoes inflict more human suffering than any other organism-and kill more than one million people each year. the mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the primary african malaria vector anopheles gambiae and the dengue and yellow fever vector aedes aegypti. rna interference- (rnai-) mediated gene silencing has been used to target genes of interest in both of these disease vector mosquito species. here, ...201525867635
evidence of limited polyandry in a natural population of aedes aegypti.the mosquito aedes aegypti is a vector of yellow fever, dengue, and chikungunya. control of the insect is crucial to stop the spread of dengue and chikungunya, so it is critically important to understand its mating behavior. primarily, based on laboratory behavior, it has long been assumed that ae. aegypti females mate once in their lifetime. however, multiple inseminations have been observed in semi-field and laboratory settings, and in closely related species. here, we report the first evidenc ...201525870424
sialic acid expression in the mosquito aedes aegypti and its possible role in dengue virus-vector interactions.dengue fever (df) is the most prevalent arthropod-borne viral disease which affects humans. df is caused by the four dengue virus (denv) serotypes, which are transmitted to the host by the mosquito aedes aegypti that has key roles in denv infection, replication, and viral transmission (vector competence). mosquito saliva also plays an important role during denv transmission. in this study, we detected the presence of sialic acid (sia) in aedes aegypti tissues, which may have an important role du ...201525874215
silencing the buzz: a new approach to population suppression of mosquitoes by feeding larvae double-stranded rnas.mosquito-borne diseases threaten over half the world's human population, making the need for environmentally-safe mosquito population control tools critical. the sterile insect technique (sit) is a biological control method that can reduce pest insect populations by releasing a large number of sterile males to compete with wild males for female mates to reduce the number of progeny produced. typically, males are sterilized using radiation, but such methods can reduce their mating competitiveness ...201525880645
mechanistic basis for morphological damage induced by essential oil from brazilian pepper tree, schinus terebinthifolia, on larvae of stegomyia aegypti, the dengue vector.dengue has become the subject of public health programs worldwide. the lack of a vaccine and the high environmental risk of synthetic insecticides, arouse the interest in natural products against this vector. this study aimed to determine the chemical composition of the essential oil of ripe fruits and seeds of schinus terebinthifolia raddi; to evaluate the essential oil effect on mortality of stegomyia aegypti (linnaeus, 1792) larvae; and to characterize the structural damage suffered by larvae ...201525886180
contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors aedes aegypti and aedes albopictus from malaysia.knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. here, we report an extensive resistance profiling of the dengue vectors aedes aegypti and aedes albopictus across malaysia and establish the contribution of knockdown resistance mechanism revealing significant contrast between both species.201525888775
vector competence of the aedes aegypti population from santiago island, cape verde, to different serotypes of dengue virus.dengue is an arboviral disease caused by dengue virus (denv), whose main vectors are the mosquitoes aedes aegypti and aedes albopictus. a. aegypti is the only denv vector in cape verde, an african country that suffered its first outbreak of dengue in 2009. however, little is known about the variation in the level of vector competence of this mosquito population to the different denv serotypes. this study aimed to evaluate the vector competence of a. aegypti from the island of santiago, cape verd ...201525888847
density of aedes aegypti and aedes albopictus and its association with number of residents and meteorological variables in the home environment of dengue endemic area, são paulo, brazil.measure the populations of ae. aegypti and ae. albopictus adults according to sex and location inside or outside the residence, estimate ae. aegypti female density per house and per resident, and test the association with abiotic factors.201525890384
spatio-temporal distribution of aedes aegypti (diptera: culicidae) mitochondrial lineages in cities with distinct dengue incidence rates suggests complex population dynamics of the dengue vector in colombia.aedes aegypti is the primary vector of the four serotypes of dengue virus (denv1-4), chikungunya and yellow fever virus to humans. previous population genetic studies have revealed a particular genetic structure among the vector populations in the americas that suggests differences in the ability to transmit denv. in colombia, despite its high epidemiologic importance, the genetic population structure and the phylogeographic depiction of ae. aegypti, as well as its relationship with the epidemio ...201525893246
susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (diptera: culicidae) from muang district, phitsanulok province, thailand.to investigate the susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (ae. aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in phitsanulok province, thailand.201525901918
effect of repeat human blood feeding on wolbachia density and dengue virus infection in aedes aegypti.the introduction of the endosymbiotic bacterium, wolbachia into aedes aegypti populations is a novel approach to reduce disease transmission. the presence of wolbachia limits the ability of the mosquito to transmit dengue virus (denv) and the strength of this effect appears to correlate with wolbachia densities in the mosquito. there is also some evidence that wolbachia densities may increase following the consumption of a bloodmeal. here we have examined whether multiple blood feeds lead to inc ...201525903749
from lab to field: the influence of urban landscapes on the invasive potential of wolbachia in brazilian aedes aegypti mosquitoes.the symbiotic bacterium wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where ...201525905888
a bayesian hierarchical model for estimation of abundance and spatial density of aedes aegypti.strategies to minimize dengue transmission commonly rely on vector control, which aims to maintain ae. aegypti density below a theoretical threshold. mosquito abundance is traditionally estimated from mark-release-recapture (mrr) experiments, which lack proper analysis regarding accurate vector spatial distribution and population density. recently proposed strategies to control vector-borne diseases involve replacing the susceptible wild population by genetically modified individuals' refractory ...201525906323
virulency of novel nanolarvicide from trichoderma atroviride against aedes aegypti (linn.): a clsm analysis.aedes aegypti is the vector for transmitting dengue, chikungunya, and yellow fever. these diseases' transmission has increased predominantly in urban and semi-urban areas as a major public health concern. in present investigation, trichoderma atroviride culture filtrates were used for the synthesis of silver nanoparticle. moreover, t. atroviride is a free-living and rapidly growing fungi common in soil and root ecosystem. this fungi is an exceptionally good model for biocontrol and more signific ...201525907629
di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426).pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ...201525912083
a neuron-specific antiviral mechanism prevents lethal flaviviral infection of mosquitoes.mosquitoes are natural vectors for many etiologic agents of human viral diseases. mosquito-borne flaviviruses can persistently infect the mosquito central nervous system without causing dramatic pathology or influencing the mosquito behavior and lifespan. the mechanism by which the mosquito nervous system resists flaviviral infection is still largely unknown. here we report that an aedes aegypti homologue of the neural factor hikaru genki (aahig) efficiently restricts flavivirus infection of the ...201525915054
an improved odor bait for monitoring populations of aedes aegypti-vectors of dengue and chikungunya viruses in kenya.effective surveillance and estimation of the biting fraction of aedes aegypti is critical for accurate determination of the extent of virus transmission during outbreaks and inter-epidemic periods of dengue and chikungunya fever. here, we describe the development and use of synthetic human odor baits for improved sampling of adult ae. aegypti, in two dengue and chikungunya fevers endemic areas in kenya; kilifi and busia counties.201525924877
larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in millettia pinnata seed toward three mosquito species.aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit dengue fever and west nile virus diseases, respectively. this study was conducted to determine the toxicity and mechanism of action of four flavonoids and two fatty acids from millettia pinnata (fabaceae) seed as well as six pure fatty acids and four fatty acid esters toward third instar larvae from insecticide-susceptible c. pipiens pallens and a. aegypti as well as wild a. albopictus. efficacy of 12 experimental l ...201525928224
costly inheritance and the persistence of insecticide resistance in aedes aegypti populations.global emergence of arboviruses is a growing public health concern, since most of these diseases have no vaccine or prevention treatment available. in this scenario, vector control through the use of chemical insecticides is one of the most important prevention tools. nevertheless, their effectiveness has been increasingly compromised by the development of strong resistance observed in field populations, even in spite of fitness costs usually associated to resistance. using a stage-structured de ...201525933383
mass trapping with mosquitraps does not reduce aedes aegypti abundance.the objective of this study was to evaluate the effectiveness of aedes aegypti mass trapping using the sticky trap mosquitrap (mqt) by performing a cluster randomised controlled trial in manaus, state of amazonas, brazil. after an initial questionnaire and baseline monitoring of adult ae. aegypti abundance with bg-sentinel (bgs) traps in six clusters, three clusters were randomly assigned to the intervention arm where each participating household received three mqts for mass trapping during 17 m ...201525946154
waiting for chikungunya fever in argentina: spatio-temporal risk maps.chikungunya virus (chikv) transmission has been detected in america in 2013 and recently reached south up to bolivia, brazil and paraguay, bordering countries of argentina. the presence of the mosquito aedes aegypti in half of the country together with the regional context drove us to make a rapid assessment of transmission risk. temperature thresholds for vector breeding and for virus transmission, together with adult activity from the literature, were mapped on a monthly basis to estimate risk ...025946252
chemical composition and larvicidal activity of essential oils extracted from brazilian legal amazon plants against aedes aegypti l. (diptera: culicidae).the mosquito aedes aegypti l. (diptera: culicidae) is the major vector of dengue and chikungunya fever. the lack of effective therapies and vaccines for these diseases highlights the need for alternative strategies to control the spread of virus. therefore, this study investigated the larvicidal potential of essential oils from common plant species obtained from the chapada das mesas national park, brazil, against third instar a. aegypti larvae. the chemical composition of these oils was determi ...201525949264
the burden of dengue and chikungunya worldwide: implications for the southern united states and california.dengue virus (denv) spreads to humans through the bite of an infected aedes aegypti or aedes albopictus mosquito and is a growing public health threat to both industrialized and developing nations worldwide. outbreaks of autochthonous dengue in the united states occurred extensively in the past but over the past 3 decades have again taken place in florida, hawaii, and texas as well as in american samoa, guam, northern mariana islands, puerto rico, and the us virgin islands. as the aedes vectors ...025960096
morbidity rate prediction of dengue hemorrhagic fever (dhf) using the support vector machine and the aedes aegypti infection rate in similar climates and geographical areas.in the past few decades, several researchers have proposed highly accurate prediction models that have typically relied on climate parameters. however, climate factors can be unreliable and can lower the effectiveness of prediction when they are applied in locations where climate factors do not differ significantly. the purpose of this study was to improve a dengue surveillance system in areas with similar climate by exploiting the infection rate in the aedes aegypti mosquito and using the suppo ...201525961289
imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito stegomyia aegypti (=aedes aegypti).the mosquito stegomyia aegypti (=aedes aegypti) (diptera: culicidae) is a vector for the dengue and yellow fever viruses. as blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. the effects of the insecticide imidacloprid on the survival of st. aegypti were investigated and the sub-lethal effects of the insecticide on midgut development were determined. third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 ...201525968596
larvicidal activity against aedes aegypti of foeniculum vulgare essential oils from portugal and cape verde.dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. this major public health problem has recurrent epidemics in latin america and occurred recently in cape verde and madeira island. the lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. essential oil (eo) constituents can affect insect's behaviour, being potentially effective in pest control. ...201525973508
sero-prevalence of dengue infections in the kassala state in the eastern part of the sudan in 2011.dengue fever is a vector-borne disease that is transmitted to humans by infected aedes aegypti mosquitoes. the eastern part of the sudan is one of the regions that is affected by dengue virus circulation. in this study, we estimated the prevalence of dengue infections in the kassala state in the eastern part of the sudan. the study objective was to estimate the sero-prevalence of dengue igg/igm antibodies in the kassala locality in 2011. this was a cross sectional community-based study that util ...201525975993
mutual exclusion of asaia and wolbachia in the reproductive organs of mosquito vectors.wolbachia is a group of intracellular maternally inherited bacteria infecting a high number of arthropod species. their presence in different mosquito species has been largely described, but aedes aegypti, the main vector of dengue virus, has never been found naturally infected by wolbachia. similarly, malaria vectors and other anophelines are normally negative to wolbachia, with the exception of an african population where these bacteria have recently been detected. asaia is an acetic acid bact ...201525981386
synergistic action of octopamine receptor agonists on the activity of selected novel insecticides for control of dengue vector aedes aegypti (diptera: culicidae) mosquito.studying insecticide resistance in mosquitoes has attracted the attention of many scientists to elucidate the pathways of resistance development and to design novel strategies in order to prevent or minimize the spread and evolution of resistance. here, we tested the synergistic action of piperonyl butoxide (pbo) and two octopamine receptor (or) agonists, amitraz (amz) and chlordimeform (cdm) on selected novel insecticides to increase their lethal action on the fourth instar larvae of aedes aegy ...201525987220
weather variability associated with aedes (stegomyia) aegypti (dengue vector) oviposition dynamics in northwestern argentina.this study aims to develop a forecasting model by assessing the weather variability associated with seasonal fluctuation of aedes aegypti oviposition dynamic at a city level in orán, in northwestern argentina. oviposition dynamics were assessed by weekly monitoring of 90 ovitraps in the urban area during 2005-2007. correlations were performed between the number of eggs collected weekly and weather variables (rainfall, photoperiod, vapor pressure of water, temperature, and relative humidity) with ...201525993415
annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector.mosquitoes transmit several diseases which cause millions of deaths every year. the use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. the present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., aedes aegypti, anopheles stephensi, and culex quinquefasciatus. t ...201526002825
blood serum and bsa, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector aedes aegypti.aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. a major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potenti ...201526020000
spatial models for prediction and early warning of aedes aegypti proliferation from data on climate change and variability in cuba.climate variability, the primary expression of climate change, is one of the most important environmental problems affecting human health, particularly vector-borne diseases. despite research efforts worldwide, there are few studies addressing the use of information on climate variability for prevention and early warning of vector-borne infectious diseases.201526027583
a threshold analysis of dengue transmission in terms of weather variables and imported dengue cases in australia.dengue virus (denv) transmission in australia is driven by weather factors and imported dengue fever (df) cases. however, uncertainty remains regarding the threshold effects of high-order interactions among weather factors and imported df cases and the impact of these factors on autochthonous df. a time-series regression tree model was used to assess the threshold effects of natural temporal variations of weekly weather factors and weekly imported df cases in relation to incidence of weekly auto ...201326038449
human-mediated marine dispersal influences the population structure of aedes aegypti in the philippine archipelago.dengue virus (denv) is an extraordinary health burden on global scale, but still lacks effective vaccine. the philippines is endemic for dengue fever, but massive employment of insecticides favored the development of resistance mutations in its major vector, aedes aegypti. alternative vector control strategies consist in releasing artificially modified mosquitos in the wild, but knowledge on their dispersal ability is necessary for a successful implementation. despite being documented that ae. a ...201526039311
dengue virus detection in aedes aegypti larvae from southeastern brazil.the transmission of dengue, the most important arthropod-borne viral disease in brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its aedes vectors. in the present study, we mapped dengue vectors in ouro preto and ouro branco, minas gerais, by installing ovitraps in 32 public schools. the traps were examined monthly between september, 2011 through july, 2012 and november, 2012 to april, 2013. the larvae were reared until the fourth stadiu ...201526047186
development and validation of an arthropod maceration protocol for zoonotic pathogen detection in mosquitoes and fleas.arthropod-borne diseases remain a pressing international public health concern. while progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qpcr), or even hand-held detection devices, a simple and robust maceration and nucleic acid extraction method is necessary to implement biosurveillance capabilities. in this study, a comparison of maceration techniques using five types of beads followed by nucleic acid extraction and detection were tested usin ...201526047188
lethal ovitrap deployment for aedes aegypti control: potential implications for non-target organisms.in australia, dengue control combines source reduction with lethal ovitraps to reduce aedes aegypti populations during outbreaks. lethal ovitraps are considered a sustainable and environmentally friendly method of controlling container-inhabiting mosquitoes, however, to-date, this claim has not been quantified. this study assesses the potential impact of lethal ovitraps on non-target organisms when used to control ae. aegypti in tropical australia. for retention of specimens, we substituted stan ...201526047194
targeted release and fractionation reveal glucuronylated and sulphated n- and o-glycans in larvae of dipteran insects.mosquitoes are important vectors of parasitic and viral diseases with anopheles gambiae transmitting malaria and aedes aegypti spreading yellow and dengue fevers. using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction hplc fractionation followed by maldi-tof ms or permethylation followed by nsi-ms), we examined the n-glycans of both a. gambiae and a. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and ...201526047717
proteomic analysis of immunogenic proteins from salivary glands of aedes aegypti.humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. this reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. our previous study identified two immunogenic proteins with molecular weights of 31 kda and 56 kda from salivary gland extract (sge) of aedes aegypti that cross-reacted with serum samples from dengue hemorrhagic fever (dhf) patients ...201526054892
characterization of the doublesex gene within the culex pipiens complex suggests regulatory plasticity at the base of the mosquito sex determination cascade.the doublesex gene controls somatic sexual differentiation of many metazoan species, including the malaria mosquito anopheles gambiae and the dengue and yellow fever vector aedes aegypti (diptera: culicidae). as in other studied dipteran dsx homologs, the gene maintains functionality via evolutionarily conserved protein domains and sex-specific alternative splicing. the upstream factors that regulate splicing of dsx and the manner in which they do so however remain variable even among closely re ...201526058583
abundant aedes (stegomyia) aegypti aegypti mosquitoes in the 2014 dengue outbreak area of mozambique.in early 2014, dengue cases were reported from northern mozambique, 30 years after the last outbreak. we identified potential dengue vector species in three northern towns, pemba, nampula and nacala, and one southern town, maputo, during the outbreak in april 2014. a major dengue vector species, aedes (stegomyia) aegypti, was found in all these towns. the dominant vector subspecies in the northern towns was aedes aegypti aegypti, while ae. aegypti formosus was dominant in maputo. considering the ...201526060423
green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti.dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ...201526063530
relationship between insecticide resistance and kdr mutations in the dengue vector aedes aegypti in southern china.aedes aegypti is an important vector for dengue virus and thus has been targeted with pyrethroid insecticides in many areas of the world. as such, resistance has been detected to several of these insecticides, including in china, but the mechanisms of the resistance are not well understood in this country.201526068925
larvicidal activity of magnolia denudata seed hydrodistillate constituents and related compounds and liquid formulations towards two susceptible and two wild mosquito species.anopheles sinensis, aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit malaria, dengue fever and west nile virus diseases respectively. this study was conducted to determine the toxicity of 17 constituents from magnolia denudata seed hydrodistillate (md-shd) and four experimental md-shd liquid formulations (10-50 mg l(-1) liquid) to third-instar larvae from insecticide-susceptible cx. p. pallens and ae. aegypti as well as wild ae. albopictus and an. sinensis.201626085316
the potential use of wolbachia-based mosquito biocontrol strategies for japanese encephalitis.japanese encephalitis virus (jev) is a zoonotic pathogen transmitted by the infectious bite of culex mosquitoes. the virus causes the development of the disease japanese encephalitis (je) in a small proportion of those infected, predominantly affecting children in eastern and southern asia. annual je incidence estimates range from 50,000-175,000, with 25%-30% of cases resulting in mortality. it is estimated that 3 billion people live in countries in which jev is endemic. the virus exists in an e ...201526086337
thiosemicarbazones as aedes aegypti larvicidal.a set of aryl- and phenoxymethyl-(thio)semicarbazones were synthetized, characterized and biologically evaluated against the larvae of aedes aegypti (a. aegypti), the vector responsible for diseases like dengue and yellow fever. (q)sar studies were useful for predicting the activities of the compounds not included to create the qsar model as well as to predict the features of a new compound with improved activity. docking studies corroborated experimental evidence of aescp-2 as a potential targe ...201526087027
predation by asian bullfrog tadpoles, hoplobatrachus tigerinus, against the dengue vector, aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles.aedes aegypti is a primary vector of dengue and chikungunya. the use of synthetic insecticides to control aedes populations often leads to high operational costs and adverse non-target effects. botanical extracts have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles, but their impact against predators of mosquito larvae has not been well studied. we propose a single-step method for the biosynthesis of silver nanoparticles (agnp) using the extract of artemisia vulgar ...201526091763
surveillance of adult aedes mosquitoes in selangor, malaysia.to determine the effectiveness of using sticky traps and the ns1 dengue antigen kit for the surveillance of aedes mosquitoes for dengue control.201526094839
characterization and bioassay for larvicidal activity of anacardium occidentale (cashew) shell waste fractions against dengue vector aedes aegypti.recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against aedes aegypti larvae. thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of anacardium occidentale shell wastes against the third and fourth instar larvae of a. aegypti and to compare the results with the commercial larvicide product. the shell wastes were e ...201526099240
proteomic changes occurring in the malaria mosquitoes anopheles gambiae and anopheles stephensi during aging.the age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. we investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes anopheles gambiae and anopheles stephensi as they aged. protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption i ...201526100052
[dengue in peru: a quarter century after its reemergence].a health problem each time more frequent and dispersed in tropical and subtropical areas of the world, including peru where it entered in 1990, is dengue. it is produced by the dengue virus with four serotypes and transmitted by aedes aegypti, a vector that coexists with humans and whose presence is favored by deficient sanitary, social and economic conditions. manifestations of severe forms of the disease such as shock and bleeding, are related to the frequent co-circulation of the four serotyp ...201526102118
[lessons learned in the control of aedes aegypti to address dengue and the emergency of chikungunya in iquitos, peru].dengue has affected iquitos since 1990 causing outbreaks of major impact on public health and for this reason great efforts have been made for its temporal control. currently, with the expansion of the chikungunya virus in the americas and the threat of the emergence of the virus in iquitos, we reflect on lessons learned by way of the activities undertaken in the area of vector control; epidemiological surveillance, diagnosis and clinical management during periods of outbreaks of dengue, in a wa ...201526102121
[vertical transmission of dengue virus in aedes aegypti, peru]. 201526102125
the epidemiology of dengue fever in saudi arabia: a systematic review.dengue fever (df) is the most serious mosquito-borne viral disease worldwide. df is an acute febrile illness caused by aedes aegypti and aedes albopictus, which are endemic in certain cities of saudi arabia, such as jeddah and makkah (mecca). an online literature search was conducted using relevant keywords to retrieve df studies conducted in saudi arabia. forty-five articles were identified initially. after screening for exclusion and retrieving full texts, a total of 10 articles were used for ...201626106040
standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus (diptera: culicidae) - ii - egg storage and hatching.management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a sterile insect technique component. the efficiency of different hatching media and effectiveness of long term storage methods are presented here.201526112698
wolbachia reduces the transmission potential of dengue-infected aedes aegypti.dengue viruses (denv) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. the wolbachia strain wmel, which has been introduced into the mosquito vector, aedes aegypti, has been shown to invade and spread to near fix ...201526115104
detection of dengue virus in individual aedes aegypti mosquitoes in delhi, india.delhi, the capital city of india, has so far witnessed several outbreaks of dengue fever since 1967 (last one reported in 2013). improved virological and entomological surveillance are the only tools that can help in prevention of dengue as well as in the development of dengue control programmes. the aim of the study was to conduct a prospective field study to detect dengue virus in adult aedes aegypti mosquitoes collected from various localities represented by different socioeconomic groups in ...201526119544
susceptibility status of aedes aegypti (l.) (diptera: culicidae) to temephos from three districts of tamil nadu, india.dengue is the most rapidly expanding arboviral disease in india. aedes aegypti is the primary vector of dengue fever. chemical insecticides have long been used in the vector control programmes along with other control measures. however, continuous use of insecticides targeting ae. aegypti may lead to development of insecticide resistance. though resistance in ae. aegypti has been reported, the mutation in ace-1 gene associated with temephos resistance is not reported in natural populations. the ...201526119549
different serotypes of dengue virus (denv) imported by polish travellers from dengue endemic areas to poland.dengue viruses are the most widespread arboviruses (transmitted mainly by aedes aegypti and ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. there are four dengue virus serotypes: denv-1, denv-2, denv-3, and denv-4. subsequent infections increase the risk of developing severe dengue fever.201526119675
disruption of dengue virus transmission by mosquitoes.current control efforts for mosquito-borne arboviruses focus on mosquito control involving insecticide applications, which are becoming increasingly ineffective and unsustainable in urban areas. mosquito population replacement is an alternative arbovirus control concept aiming at replacing virus-competent vector populations with laboratory-engineered incompetent vectors. a prerequisite for this strategy is the design of robust anti-pathogen effectors that can ultimately be genetically driven thr ...026120563
current status of invasive mosquito surveillance in the uk.non-native invasive mosquitoes have for many years made incursions into europe, and are now established in many european countries. the continued european importation of potential vectors and their expansion within europe increases their potential for importation and establishment in the uk. coupled with increasing numbers of returning dengue and chikungunya infected travellers, the potential exists for transmission of vector borne disease in new regions.201526122427
an environmentally safe larvicide against aedes aegypti based on in situ gelling nanostructured surfactant systems containing an essential oil.liquid crystalline precursors, which are in situ gelling nanostructured surfactant systems, can undergo phase transition in aqueous solution and become more structured aggregates, controlling release of larvicides and acting as biotechnology alternatives for dengue control. such systems can contain bioactive substances as citrus sinensis essential oil (cseo) which exhibits biological activity against aedes aegypti (ae. aegypti) larvae.201526125515
the global distribution of the arbovirus vectors aedes aegypti and ae. albopictus.dengue and chikungunya are increasing global public health concerns due to their rapid geographical spread and increasing disease burden. knowledge of the contemporary distribution of their shared vectors, aedes aegypti and aedes albopictus remains incomplete and is complicated by an ongoing range expansion fuelled by increased global trade and travel. mapping the global distribution of these vectors and the geographical determinants of their ranges is essential for public health planning. here ...201526126267
suppression of a field population of aedes aegypti in brazil by sustained release of transgenic male mosquitoes.the increasing burden of dengue, and the relative failure of traditional vector control programs highlight the need to develop new control methods. sit using self-limiting genetic technology is one such promising method. a self-limiting strain of aedes aegypti, ox513a, has already reached the stage of field evaluation. sustained releases of ox513a ae. aegypti males led to 80% suppression of a target wild ae. aegypti population in the cayman islands in 2010. here we describe sustained series of f ...201526135160
emergence of dengue in tribal villages of mandla district, madhya pradesh, india.dengue (den) is a rapidly spreading arboviral disease transmitted by aedes mosquitoes. although it is endemic in india, dengue virus (denv) infection has not been reported from tribal areas of madhya pradesh. investigations were conducted to establish the aetiology of sudden upsurge of cases with febrile illness in june 2013 from tribal villages of mandla district of madhya pradesh, india.026139775
[insecticide susceptibility evaluation in aedes aegypti populations of caldas, colombia, in 2007 and 2011].continuous use of insecticides for the control of dengue transmission may lead to decreased susceptibility levels in mosquito vector populations. timely monitoring is necessary to ensure detection of any potential resistance problems.201526148033
[microgeographic and temporal genetic changes of aedes aegypti from medellín, colombia].aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. the knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies.201526148034
evaluation of silver nanoparticles toxicity of arachis hypogaea peel extracts and its larvicidal activity against malaria and dengue vectors.silver nanoparticles (agnps) were successfully synthesised from aqueous silver nitrate using the extracts of arachis hypogaea peels. the synthesised snps were characterized by fourier transform-infrared spectroscopy analysis, x-ray diffraction, transmission electron microscopy analysis and high-resonance scanning electron microscopy, and energy dispersive x-ray spectroscopy. agnps were well defined and measured 20 to 50 nm in size. the nanoparticles were crystallized with a face-centered cubic s ...201526154036
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