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dengue fever occurrence and vector detection by larval survey, ovitrap and mosquitrap: a space-time clusters analysis.the use of vector surveillance tools for preventing dengue disease requires fine assessment of risk, in order to improve vector control activities. nevertheless, the thresholds between vector detection and dengue fever occurrence are currently not well established. in belo horizonte (minas gerais, brazil), dengue has been endemic for several years. from january 2007 to june 2008, the dengue vector aedes (stegomyia) aegypti was monitored by ovitrap, the sticky-trap mosquitrap™ and larval surveys ...201222848729
wolbachia induces density-dependent inhibition to dengue virus in mosquito cells.wolbachia is a maternal transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. the ability of wolbachia to both induce viral interference and spread into mosquito vector population makes it possible to develop wolbachia as a biological control agent for dengue control. while wolbachia induces resistance to dengue virus in the transinfected aedes aegypti mosquitoes, a similar effect was not observed in aedes albopictus, which naturally carries wolbachia infec ...201222848774
[a school program for dengue control in honduras: from knowledge to action].the environmental school program (pea, for its spanish acronym), a dengue control initiative focused on primary schools that took place during 2005-2010 in several cities in honduras, is described. the environmental health program was designed to increase knowledge and develop skills in the identification and control of aedes aegypti breeding sites, as well as in water and solid waste management. the results, as measured by behavioral change and reduced larval indices, were satisfactory in the m ...201222858820
low entomological impact of new water supply infrastructure in southern vietnam, with reference to dengue vectors.we did a prospective study in southern vietnam where new water infrastructure was added. new 1,200-l tanks may present potential breeding grounds for aedes aegypti, particularly when sealed lids were not always supplied. some householders in these communes received a piped water supply, however there was no reduction in water storage practices. the prevalence of aedes aegypti immatures in tank and tap households reached 73%, but were non-significantly different from each other and from control h ...201222869632
expression profile of genes during resistance reversal in a temephos selected strain of the dengue vector, aedes aegypti.the mosquito aedes aegypti is one of the most important disease vectors because it transmits two major arboviruses, dengue and yellow fever, which cause significant global morbidity and mortality. chemical insecticides form the cornerstone of vector control. the organophosphate temephos a larvicide recommended by who for controlling ae. aegypti, however, resistance to this compound has been reported in many countries, including brazil.201222870187
immune transcript variations among aedes aegypti populations with distinct susceptibility to dengue virus serotype 2.the innate immune response of insects is one of the factors that may dictate their susceptibility to viral infection. two immune signaling pathways, toll and jak-stat, and the rna interference (rnai) pathway are involved in aedes aegypti responses against dengue virus (denv), however natural differences in these antiviral defenses among mosquito populations have not been studied. here, two field ae. aegypti populations from distinct ecological environments, one from recife and the other from pet ...201222877626
update on temporal and spatial abundance of dengue vectors in penang, malaysia.it is important to obtain frequent measurements of the abundance, distribution, and seasonality of mosquito vectors to determine the risk of disease transmission. the number of cases of dengue infection has increased in recent years on penang island, malaysia, with recurring epidemics. however, ongoing control attempts are being critically hampered by the lack of up-to-date information regarding the vectors. to overcome this problem, we examined the current situation and distribution of dengue v ...201222894118
mosquito vector biology and control in latin america--a 22nd symposium.the 22nd annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 78th annual meeting in austin, tx, in february 2012. the principal objective, as for the previous 21 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 21 presentations that were given orally in spanish or presented as posters by participants ...201222894120
vertebrate hosts of aedes aegypti and aedes mediovittatus (diptera: culicidae) in rural puerto rico.the distribution of aedes (stegomyia) aegypti (l.), the main vector of dengue viruses (denv) worldwide, overlaps with aedes (gymnometopa) mediovittatus (coquillett), the caribbean treehole mosquito, in urban, suburban, and rural areas. ae. mediovittatus is a competent vector of denv with high rates of vertical denv transmission in the laboratory. this study determined whether ae. mediovittatus feeds on humans and compared its feeding patterns with co-occurring ae. aegypti in two rural communitie ...022897052
vector competence of aedes aegypti and aedes albopictus (diptera: culicidae) for dengue virus in the florida keys.in 2009-2011, monroe county in southern florida experienced locally acquired and traveler-imported focal dengue outbreaks. aedes aegypti (l.) is the primary vector of dengue virus (denv) worldwide, is prevalent in monroe county, and is the suspected vector in florida. ae. albopictus (skuse) is also known to be an important vector of denv and this species is ubiquitous in florida; however, it is not yet established in monroe county. florida ae. aegypti (key west and stock island geographic coloni ...201222897056
field comparison of thermal and non-thermal ultra-low-volume applications using water and diesel as solvents for managing dengue vector, aedes aegypti.to compare the effectiveness on aedes aegypti (linneo) (diptera: culicidae) of a larvicide-adulticide ulv formulation applied by a thermal or a cold fogger using different solvents.201222906100
impact of environmental temperatures on resistance to organophosphate insecticides in aedes aegypti from trinidad.to examine the effects of increasing larval rearing temperatures on the resistance status of trinidadian populations of aedes aegypti to organophosphate (op) insecticides.201222910718
oral susceptibility of singapore aedes (stegomyia) aegypti (linnaeus) to zika virus.zika virus (zikv) is a little known flavivirus that caused a major outbreak in 2007, in the south-western pacific island of yap. it causes dengue-like syndromes but with milder symptoms. in africa, where it was first isolated, zikv is mainly transmitted by sylvatic aedes mosquitoes. the virus has also been isolated from ae. aegypti and it is considered to be the vector involved in the urban transmission of the virus. transmission of the virus by an african strain of ae. aegypti has also been dem ...201222953014
the "auto-dissemination" approach: a novel concept to fight aedes albopictus in urban areas.the main constraint to the fight against container-breeding mosquito vectors of human arboviruses is the difficulty in targeting the multiplicity of larval sources, mostly represented by small man-made water containers. the aim of this work is to assess the feasibility of the "auto-dissemination" approach, already tested for aedes aegypti, as a possible alternative to traditional, inefficient control tools, against ae. albopictus in urban areas. the approach is based on the possibility that wild ...201222953015
the interactive roles of aedes aegypti super-production and human density in dengue transmission.a. aegypti production and human density may vary considerably in dengue endemic areas. understanding how interactions between these factors influence the risk of transmission could improve the effectiveness of the allocation of vector control resources. to evaluate the combined impacts of variation in a. aegypti production and human density we integrated field data with simulation modeling.201222953017
evaluation of a sticky trap (aedestrap), made from disposable plastic bottles, as a monitoring tool for aedes aegypti populations.dengue virus, which is transmitted by aedes aegypti mosquitoes is the most important emerging viral disease, infecting more than 50 million people annually. currently used sticky traps are useful tools for monitoring and control of a. aegypti, despite differences in efficiency, labor requirements and cost. in the present work, a field assay was carried out to evaluate the performance of a sticky trap (aedestrap), produced using disposable material, in capturing gravid aedes spp. females. additio ...201222958376
open field release of genetically engineered sterile male aedes aegypti in malaysia.dengue is the most important mosquito-borne viral disease. in the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, aedes aegypti, yet current methods have not proven adequate to control the disease. new methods are therefore urgently needed, for example genetics-based sterile-male-release methods. however, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field.201222970102
the dengue virus mosquito vector aedes aegypti at high elevation in mexico.méxico has cities (e.g., méxico city and puebla city) located at elevations > 2,000 m and above the elevation ceiling below which local climates allow the dengue virus mosquito vector aedes aegypti to proliferate. climate warming could raise this ceiling and place high-elevation cities at risk for dengue virus transmission. to assess the elevation ceiling for ae. aegypti and determine the potential for using weather/climate parameters to predict mosquito abundance, we surveyed 12 communities alo ...201222987656
associative learning in the dengue vector mosquito, aedes aegypti: avoidance of a previously attractive odor or surface color that is paired with an aversive stimulus.associative learning has been shown in a variety of insects, including the mosquitoes culex quinquefasciatus and anopheles gambiae. this study demonstrates associative learning for the first time in aedes aegypti, an important vector of dengue, yellow fever and chikungunya viruses. this species prefers to rest on dark surfaces and is attracted to the odor of 1-octen-3-ol. after training in which a dark surface alone or a dark surface with odor was paired with electric shock, mosquitoes avoided t ...201222996441
field evaluations of disposable sticky lures for surveillance of aedes aegypti (stegomyia aegypti) and culex quinquefasciatus in jakarta.from december 1997 to april 1998, disposable sticky lures (1608 lure days) were trialled in homes in north jakarta, indonesia as surveillance tools for aedes aegypti (stegomyia aegypti) (diptera: culicidae) and culex quinquefasciatus (diptera: culicidae), referenced to indoor resting adult collections (92 × 10 min). the lures collected 89.4% of the total of 1339 ae. aegypti and 92.1% of the total of 1272 cx. quinquefasciatus collected by all methods. because there were no significant differences ...201323002913
rapid intraspecific evolution of mirna and sirna genes in the mosquito aedes aegypti.rna silencing, or rna interference (rnai) in metazoans mediates development, reduces viral infection and limits transposon mobility. rna silencing involves 21-30 nucleotide rnas classified into microrna (mirna), exogenous and endogenous small interfering rnas (sirna), and piwi-interacting rna (pirna). knock-out, silencing and mutagenesis of genes in the exogenous sirna (exo-sirna) regulatory network demonstrate the importance of this rnai pathway in antiviral immunity in drosophila and mosquitoe ...201223028502
spatial and temporal patterns of dengue transmission along a red sea coastline: a longitudinal entomological and serological survey in port sudan city.dengue is an emerging health problem in several coastlines along the red sea. the objective of the present work is to elucidate spatial and temporal patterns of dengue transmission in port sudan.201223029582
laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of malaria, dengue/dengue hemorrhagic fever (df/dhf) and lymphatic filariasis in the semi-arid desert.comparative larvicidal efficacy of aqueous and organic solvent extracts from seeds, leaves and flowers of three desert plants viz. calotropis procera (aiton), tephrosia purpurea (l.) pers. and prosopis juliflora (sw.) dc. was evaluated against anopheles stephensi (liston), aedes aegypti (linnaeus) and culex quinquefasciatus (say). for this purpose larvae of all the three mosquito species were reared in the laboratory and studies carried out on late 3rd or early 4th instars using standard who tec ...201223029912
diversity of containers and buildings infested with aedes aegypti in puerto iguazú, argentina.aedes aegypti is the main domestic vector of the dengue virus. control measures to prevent dengue transmission focus on the treatment and elimination of this vector's oviposition sites. there is limited biological information on ae. aegypti in argentina. the aim of this study was to characterize ae. aegypti oviposition sites in the city of puerto iguazú, argentina. we surveyed an area covering nine neighborhoods in 2005. we identified 191 premises as positive for ae. aegypti, giving a general ho ...201223033195
diarrhea and dengue control in rural primary schools in colombia: study protocol for a randomized controlled trial.diarrheal diseases and dengue fever are major global health problems. where provision of clean water is inadequate, water storage is crucial. fecal contamination of stored water is a common source of diarrheal illness, but stored water also provides breeding sites for dengue vector mosquitoes. poor household water management and sanitation are therefore potential determinants of both diseases. little is known of the role of stored water for the combined risk of diarrhea and dengue, yet a joint r ...201223034084
the history of dengue outbreaks in the americas.dengue is a viral disease usually transmitted by aedes aegypti mosquitoes. dengue outbreaks in the americas reported in medical literature and to the pan american health organization are described. the outbreak history from 1600 to 2010 was categorized into four phases: introduction of dengue in the americas (1600-1946); continental plan for the eradication of the ae. aegypti (1947-1970) marked by a successful eradication of the mosquito in 18 continental countries by 1962; ae. aegypti reinfesta ...023042846
analyzing the spatio-temporal relationship between dengue vector larval density and land-use using factor analysis and spatial ring mapping.dengue, a mosquito-borne febrile viral disease, is found in tropical and sub-tropical regions and is now extending its range to temperate regions. the spread of the dengue viruses mainly depends on vector population (aedes aegypti and aedes albopictus), which is influenced by changing climatic conditions and various land-use/land-cover types. spatial display of the relationship between dengue vector density and land-cover types is required to describe a near-future viral outbreak scenario. this ...201223043443
dengue antibodies in blood donors.dengue is an urban arbovirus whose etiologic agent is a virus of the genus flavorius with four distinct antigen serotypes (denv-1, denv-2, denv-3 and denv-4) that is transmitted to humans through the bite of the mosquito aedes aegypti. the campo mourão region in brazil is endemic for dengue fever. obtective: the aim of this study was to evaluate the presence of igg and igm antibodies specific to the four serotypes of dengue in donors of the blood donor service in the city of campo mourão.023049418
a critical role of the nuclear receptor hr3 in regulation of gonadotrophic cycles of the mosquito aedes aegypti.the orphan nuclear receptor hr3 is essential for developmental switches during insect development and metamorphosis regulated by 20-hydroxyecdysone (20e). reproduction of female mosquitoes of the major vector of dengue fever, aedes aegypti, is cyclic because of its dependence on blood feeding. 20e is an important hormone regulating vitellogenic events in this mosquito; however, any role for hr3 in 20e-driven reproductive events has not been known. using rna interference (rnai) approach, we demon ...201223049766
biosynthesized silver nanoparticles from pedilanthus tithymaloides leaf extract with anti-developmental activity against larval instars of aedes aegypti l. (diptera; culicidae).mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. aqueous leaf extract and green synthesized silver nanoparticles (ag nps) from pedilanthus tithymaloides (l.) poit. were investigated for their efficacy against the dengue vector aedes aegypti l. (diptera; culicidae). the biologically synthesized ag nps were characterized by uv-vis spectrum, x-ray diffraction, fourie ...201323052770
molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector aedes aegypti.mosquitoes are vectors of several major human diseases and their control is mainly based on the use of chemical insecticides. resistance of mosquitoes to organochlorines, organophosphates, carbamates and pyrethroids led to a regain of interest for the use of neonicotinoid insecticides in vector control. the present study investigated the molecular basis of neonicotinoid resistance in the mosquito aedes aegypti. a strain susceptible to insecticides was selected at the larval stage with imidaclopr ...201323058251
an eco-physiological model of the impact of temperature on aedes aegypti life history traits.physiological processes mediate the impact of ecological conditions on the life histories of insect vectors. for the dengue/chikungunya mosquito, aedes aegypti, three life history traits that are critical to urban population dynamics and control are: size, development rate and starvation mortality. in this paper we make use of prior laboratory experiments on each of these traits at 2°c intervals between 20 and 30°c, in conjunction with eco-evolutionary theory and studies on a.aegypti physiology, ...201223068992
aging field collected aedes aegypti to determine their capacity for dengue transmission in the southwestern united states.aedes aegypti, the primary vector of dengue virus, is well established throughout urban areas of the southwestern us, including tucson, az. local transmission of the dengue virus, however, has not been reported in this area. although many factors influence the distribution of the dengue virus, we hypothesize that one contributing factor is that the lifespan of female ae. aegypti mosquitoes in the southwestern us is too short for the virus to complete development and be transmitted to a new host. ...201223077536
comparative expression profiles of midgut genes in dengue virus refractory and susceptible aedes aegypti across critical period for virus infection.aedes aegypti is the primary mosquito vector for dengue virus (denv) worldwide. infectivity of dengue virus varies among natural populations of this mosquito. how a. aegypti responds to denv infection relative to which genes and associated pathways contribute to its differential susceptibility as a vector is not well defined.201223077596
dengue-2 virus carrying capacity of thai aedes aegypti strains with different susceptibility to deltamethrin.deltamethrin-resistant aedes aegypti currently threatens the effectiveness of dengue hemorrhagic fever control operations in thailand. although a previous study has suggested that insecticide resistance may increase ae. aegypti susceptibility to dengue-2 virus infection, our experimental data showed no significant association between laboratory-induced deltamethrin-resistance in a thai ae. aegypti isolate and its susceptibility to dengue -2 infection.201223077842
proof of concept for a novel insecticide bioassay based on sugar feeding by adult aedes aegypti (stegomyia aegypti).aedes aegypti l. (stegomyia aegypti) (diptera: culicidae) is the principal vector of dengue and yellow fever viruses in tropical and subtropical regions of the world. disease management is largely based on mosquito control achieved by insecticides applied to interior resting surfaces and through space sprays. population monitoring to detect insecticide resistance is a significant component of integrated disease management programmes. we developed a bioassay method for assessing insecticide susce ...201323077986
resistance of aedes aegypti (l.) larvae to temephos in surabaya, indonesia.the resistance of aedes aegypti mosquitoes to insecticides threatens dengue virus control efforts. in this study, ae. aegypti larvae collected from 12 subdistricts in surabaya, indonesia, where dengue is endemic, were tested for resistance to the organophosphate, temephos. susceptibility testing, performed according to world health organization (who) methods, showed all field strains were resistant to temephos at a dose of 0.012 mg/l, with mortality rates at 24 hours of 22% to 60%. another susce ...201223082551
insecticide susceptibility status of field-collected aedes (stegomyia) aegypti (l.) at a dengue endemic site in shah alam, selangor, malaysia.biweekly ovitrap surveillance (os) was conducted for a year (august 2007 - september 2008) at two different dengue endemic sites in shah alam, selangor, malaysia, 50 km from kuala lumpur. aedes aegypti collected from these 2 locations were raised to the f3 stage and subjected to a who standard bioassay method to determine lethal time (lt) against pyrethroids (permethrin 0.75%, cyfluthrin 0.15%), organophosphates (malathion 5.0%, fenitrothion 1.0%), carbamates (propoxur 0.1%, bendiocarb 0.1%) and ...201223082552
spray application of bacillus thuringiensis israelensis (bti strain am65-52) against aedes aegypti (l.) and ae. albopictus skuse populations and impact on dengue transmission in a dengue endemic residential site in malaysia.a one year study was conducted to evaluate the impact of spray application of bacillus thuringiensis israelensis (bti), strain am65-52 on vector populations and dengue transmission in a dengue endemic state in malaysia. residential sites with similar populations of aedes aegypti (l.) and aedes albopictus skuse were studied. one site was treated with spray application of bti into all outdoor target vector habitats, which consisted of natural and artificial containers. the other site was not treat ...201223082582
insecticide susceptibility of aedes aegypti populations from senegal and cape verde archipelago.two concomitant dengue 3 (den-3) epidemics occurred in cape verde archipelago and senegal between september and october 2009. aedes aegypti was identified as the vector of these epidemics as several den-3 virus strains were isolated from this species in both countries. the susceptibility to pyrethroids, organochlorine, organophosphates and carbamate was investigated in two field strains of aedes aegypti from both countries using who diagnostic bioassay kits in order to monitor their the current ...201223088621
household disposables as breeding habitats of dengue vectors: linking wastes and public health.an assessment of the household wastes as larval habitats of the dengue vectors was made considering kolkata, india, as geographical area. wastes of four major categories, namely, earthen, porcelain, plastic and coconut shells were monitored for positive with immature of either aedes aegypti or aedes albopictus. twenty six types of wastes with varying size and shape, resembling containers, were identified that hosted mosquito immature. the number of waste containers positive for aedes immature va ...201323107350
dengue vector management using insecticide treated materials and targeted interventions on productive breeding-sites in guatemala.in view of the epidemiological expansion of dengue worldwide and the availability of new tools and strategies particularly for controlling the primary dengue vector aedes aegypti, an intervention study was set up to test the efficacy, cost and feasibility of a combined approach of insecticide treated materials (itms) alone and in combination with appropriate targeted interventions of the most productive vector breeding-sites.201223110515
synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of annona muricata and piper nigrum against the dengue fever vector aedes aegypti.phytopesticide combinations of different botanical sources are seldom reported. annona muricata seed and piper nigrum fruit ethanolic extracts enriched in acetogenins and piperamides, respectively, were synergistically used as larvicides against the dengue fever vector aedes aegypti.201323112098
effect of wolbachia on replication of west nile virus in a mosquito cell line and adult mosquitoes.wolbachia as an endosymbiont is widespread in insects and other arthropods and is best known for reproductive manipulations of the host. recently, it has been shown that wmelpop and wmel strains of wolbachia inhibit the replication of several rna viruses, including dengue virus, and other vector-borne pathogens (e.g., plasmodium and filarial nematodes) in mosquitoes, providing an alternative approach to limit the transmission of vector-borne pathogens. in this study, we tested the effect of wolb ...201223115298
genetically modified mosquito: the malaysian public engagement experience.on december 21, 2010, 6000 genetically modified (gm) mosquitoes were released in an uninhabited forest in malaysia. the purpose of the deliberate release was a limited “marked release and recapture” (mrr) experiment, a standard ecological method in entomology, to evaluate under field conditions, the flight distance and longevity of the sterile male aedes aegypti strain ox513a(my1), a gm strain. as with any other gm technologies, the release was received with mixed responses. as the scientific co ...201223125042
impact of wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector aedes aegypti.incidence of disease due to dengue (denv), chikungunya (chikv) and yellow fever (yfv) viruses is increasing in many parts of the world. the viruses are primarily transmitted by aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. when transinfected into ae. aegypti, the intracellular bacterium wolbachia has recently been shown to inhibit replication of denvs, chikv, malaria parasites and filarial nematodes, providing a potentially powerful biocontrol st ...201223133693
selective inhibitors of digestive enzymes from aedes aegypti larvae identified by phage display.dengue is a serious disease transmitted by the mosquito aedes aegypti during blood meal feeding. it is estimated that the dengue virus is transmitted to millions of individuals each year in tropical and subtropical areas. dengue control strategies have been based on controlling the vector, ae. aegypti, using insecticide, but the emergence of resistance poses new challenges. the aim of this study was the identification of specific protease inhibitors of the digestive enzymes from ae. aegypti larv ...201323142191
gene flow networks among american aedes aegypti populations.the mosquito aedes aegypti, the dengue virus vector, has spread throughout the tropics in historical times. while this suggests man-mediated dispersal, estimating contemporary connectivity among populations has remained elusive. here, we use a large mtdna dataset and a bayesian coalescent framework to test a set of hypotheses about gene flow among american ae. aegypti populations. we assessed gene flow patterns at the continental and subregional (amazon basin) scales. for the americas, our data ...023144654
fitness evaluation of two brazilian aedes aegypti field populations with distinct levels of resistance to the organophosphate temephos.in brazil, decades of dengue vector control using organophosphates and pyrethroids have led to dissemination of resistance. although these insecticides have been employed for decades against aedes aegypti in the country, knowledge of the impact of temephos resistance on vector viability is limited. we evaluated several fitness parameters in two brazilian ae. aegypti populations, both classified as deltamethrin resistant but with distinct resistant ratios (rr) for temephos. the insecticide-suscep ...201223147149
dengue virus type 4 in niterói, rio de janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance.in niterói, state of rio de janeiro, dengue virus type 4 (denv-4) was isolated for the first time in march 2011. we analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of denv-4 in aedes aegypti that were field-caught. conventional reverse transcriptase-polymerase chain reaction (rt-pcr) and simplexa™ dengue real-time rt-pcr confirmed denv-4 infection in all cases. additionally, denv-4 was confirmed in a female ae. aegypti with 1.08 ...201223147153
effect of wolbachia on insecticide susceptibility in lines of aedes aegypti.two stable infections of wolbachia pipientis, wmelpop and wmel, now established in aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. any effects of wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. bioassays of ae. aegypti were conducted to test for differences in response to insecticides between wolbachia infected (wmelpop, wmel) and uninfected lines. insecticides screened were bifenthrin, the pyret ...201323149015
seasonal population dynamics and the genetic structure of the mosquito vector aedes aegypti in são paulo, brazil.population genetic studies of insect vectors can generate knowledge to improve epidemiological studies focused on the decrease of pathogen transmission. in this study, we used nine snps across the aedes aegypti genome to characterize seasonal population variations of this important dengue vector. mosquito samples were obtained by ovitraps placed over botucatu sp from 2005 to 2010. our data show that, regardless of the large variation in mosquito abundance (deduced from the number of eggs obtaine ...201223170214
salinity-tolerant larvae of mosquito vectors in the tropical coast of jaffna, sri lanka and the effect of salinity on the toxicity of bacillus thuringiensis to aedes aegypti larvae.dengue, chikungunya, malaria, filariasis and japanese encephalitis are common mosquito-borne diseases endemic to sri lanka. aedes aegypti and aedes albopictus, the major vectors of dengue, were recently shown to undergo pre-imaginal development in brackish water bodies in the island. a limited survey of selected coastal localities of the jaffna district in northern sri lanka was carried out to identify mosquito species undergoing pre-imaginal development in brackish and saline waters. the effect ...201223174003
synergistic effect of eugenia jambolana linn. and solidago canadensis linn. leaf extracts with deltamethrin against the dengue vector aedes aegypti linn. at mysore.with the goal in mind to minimize the application of environmentally hazardous chemical insecticides, the larvicidal activity of two plant extracts along with deltamethrin was studied at university of mysore. the extracts of solidago canadensis and eugenia jambolana were employed for working out the synergistic efficacy against aedes aegypti larvae, as the extracts of both the plants exhibited high efficacy when applied individually. the deltamethrin when analyzed separately, lc50 and lc90 value ...201323179216
pyrethroid resistance in aedes aegypti and aedes albopictus from port-au-prince, haiti.in port-au-prince, haiti, the status of insecticide resistance has not recently been evaluated for aedes aegypti (l) and aedes albopictus (skuse) populations. no prophylactics exist for dengue, so prevention is only through vector control methods. an earthquake occurred in haiti on january 12, 2010, with a magnitude of 7.0 mw that devastated the area. dengue became a major concern for the humanitarian relief workers that entered the country. bottle bioassays were conducted in the field on adult ...023181855
fine scale spatial urban land cover factors associated with adult mosquito abundance and risk in tucson, arizona.it is currently unclear what role microhabitat land cover plays in determining the seasonal spatial distribution of aedes aegypti and culex quinquefasciatus, disease vectors of dengue and west nile virus, respectively, in tucson, az. we compared mosquito abundance to sixteen land cover variables derived from 2010 naip multispectral data and 2008 lidar height data. mosquitoes were trapped with 30-9 traps from may to october of 2010 and 2011. variables were extracted for five buffer zones (10-50 m ...201223181866
the influence of local environment on the aging and mortality of aedes aegypti (l.): case study in fortaleza-ce, brazil.it is generally assumed that the daily probability of survival of mosquitoes is independent of age. to test this assumption we have conducted a three-year experimental fieldwork study (2005-2007) at fortaleza-ce in brazil, determining daily survival rates of the dengue vector aedes aegypti (l.). survival rates of adult ae. aegypti may be age-dependent and the statistical analysis is a sensitive approach for comparing patterns of mosquito survival. the mosquito survival data were better fit by a ...201223181868
detection of dengue virus ns1 antigen in infected aedes aegypti using a commercially available kit.epidemic dengue has emerged throughout the tropical world. in the continued absence of a vaccine against dengue virus (denv), mosquito vector surveillance and control programs are essential to reduce human infections. an effective test to detect denv in infected mosquitoes would be a valuable addition to the surveillance effort. we investigated denv detection in infected aedes aegypti using a commercially available denv non-structural protein 1 (ns1) elisa kit (platelia dengue ns1 ag), and by re ...201223185074
increase in larval gut proteolytic activities and bti resistance in the dengue fever mosquito.the bioinsecticide bacillus thuringiensis var. israelensis (bti) is increasingly used worldwide for mosquito control. although no established resistance to bti has been described in the field so far, a resistant aedes aegypti strain (litox strain) was selected in the laboratory using field-collected leaf litter containing bti toxins. this selected strain exhibits a moderate resistance level to bti, but a high resistance level to individual cry toxins. as bti contains four different toxins, gener ...201323192850
specificity of resistance to dengue virus isolates is associated with genotypes of the mosquito antiviral gene dicer-2.in contrast to the prevailing view that invertebrate immunity relies on broad-spectrum recognition and effector mechanisms, intrinsic genetic compatibility between invertebrate hosts and their pathogens is often highly specific in nature. solving this puzzle requires a better understanding of the molecular basis underlying observed patterns of invertebrate host-pathogen genetic specificity, broadly referred to as genotype-by-genotype interactions. here, we identify an invertebrate immune gene in ...023193131
evaluation of repellent activities of cymbopogon essential oils against mosquito vectors of malaria, filariasis and dengue fever in india.essential oils of four species and two hybrid varieties of cymbopogon grasses were evaluated for their repellent properties against the major vector mosquitoes, namely, anopheles stephensi, culex quinque-fasciatus and aedes aegypti, both in laboratory and field. the magnitude of repellency in the cymbopogon essential oils was found to be of moderate to high order. all grass species protected completely from mosquito bites for 4 hrs, whereas c. nardus provided protection for as much as 8-10 hrs o ...199823195906
oral receptivity of aedes aegypti from cape verde for yellow fever, dengue, and chikungunya viruses.at the end of 2009, 21,313 cases of dengue-3 virus (denv-3) were reported in the islands of cape verde, an archipelago located in the atlantic ocean 570 km from the coast of western africa. it was the first dengue outbreak ever reported in cape verde. mosquitoes collected in july 2010 in the city of praia, on the island of santiago, were identified morphologically as aedes aegypti formosus. using experimental oral infections, we found that this vector showed a moderate ability to transmit the ep ...201323199267
blood meal induced microrna regulates development and immune associated genes in the dengue mosquito vector, aedes aegypti.aedes aegypti is a blood-feeding mosquito that transmits human pathogens such as dengue virus, yellow fever virus and chikungunya virus. recently, dramatic changes in the transcriptome of ae. aegypti following a blood meal have been reported; however, the molecular factors involved in regulating these changes are largely unknown. in this study, we found induction of a number of endogenous micrornas (mirnas) in blood fed (bf) mosquitoes. one of these mirnas, aae-mir-375, was only detected in bf m ...201323202267
dendritic cell apoptosis and the pathogenesis of dengue.dengue viruses and other members of the flaviviridae family are emerging human pathogens. dengue is transmitted to humans by aedes aegypti female mosquitoes. following infection through the bite, cells of the hematopoietic lineage, like dendritic cells, are the first targets of dengue virus infection. dendritic cells (dcs) are key antigen presenting cells, sensing pathogens, processing and presenting the antigens to t lymphocytes, and triggering an adaptive immune response. infection of dcs by d ...201223202502
bdelloid rotifer, philodina species in the breeding containers of aedes aegypti and aedes albopictus.the vector mosquitoes of dengue and chikungunya fever, aedes aegypti and aedes albopictus have adapted to feed on humans and undergo larval and pupal development in natural and artificial freshwater collections. although several studies reported, still, much information is required to understand the successful survival of aedes mosquitoes in small temporary containers. in an investigation conducted in the chikungunya affected areas of kerala state, india, the presence of bdelloid rotifer, philod ...201223202612
productivity and population density estimates of the dengue vector mosquito aedes aegypti (stegomyia aegypti) in australia.new mosquito control strategies centred on the modifying of populations require knowledge of existing population densities at release sites and an understanding of breeding site ecology. using a quantitative pupal survey method, we investigated production of the dengue vector aedes aegypti (l.) (stegomyia aegypti) (diptera: culicidae) in cairns, queensland, australia, and found that garden accoutrements represented the most common container type. deliberately placed 'sentinel' containers were se ...201323205694
complex modulation of the aedes aegypti transcriptome in response to dengue virus infection.dengue fever is the most important arboviral disease world-wide, with aedes aegypti being the major vector. interactions between the mosquito host and dengue viruses (denv) are complex and vector competence varies among geographically-distinct ae. aegypti populations. additionally, dengue is caused by four antigenically-distinct viral serotypes (denv1-4), each with multiple genotypes. each virus genotype interacts differently with vertebrate and invertebrate hosts. analyses of alterations in mos ...201223209765
larval ecology of mosquitoes in sylvatic arbovirus foci in southeastern senegal.although adult mosquito vectors of sylvatic arbovirus [yellow fever (yfv), dengue-2 (denv-2) and chikungunya (chikv)] have been studied for the past 40 years in southeastern senegal, data are still lacking on the ecology of larval mosquitoes in this area. in this study, we investigated the larval habitats of mosquitoes and characterized their seasonal and spatial dynamics in arbovirus foci.201223216815
a review of dengue as an emerging disease in pakistan.the presence of dengue virus has been detected using neutralization and haemagglutination inhibition antibodies in local populations in pakistan since the 1960s. however, the first epidemic was not reported until 1994. this was followed by some cases in 1995, but the disease was confined to the port city of karachi. since 2006, dengue epidemics have occurred every year and the range has extended to most cities in pakistan. dengue now affects thousands of people and has caused hundreds of deaths. ...201323219263
ongoing outbreak of dengue type 1 in the autonomous region of madeira, portugal: preliminary report.following the identification of two autochthonous cases of dengue type 1 on 3 october 2012, an outbreak of dengue fever has been reported in madeira, portugal. as of 25 november, 1,891 cases have been detected on the island where the vector aedes aegypti had been established in some areas since 2005. this event represents the first epidemic of dengue fever in europe since 1928 and concerted control measures have been initiated by local health authorities.201223231893
targeting gene expression to the female larval fat body of transgenic aedes aegypti mosquitoes.as the fat body is a critical tissue for mosquito development, metamorphosis, immune and reproductive system function, the characterization of regulatory modules targeting gene expression to the female mosquito fat body at distinct life stages is much needed for multiple, varied strategies for controlling vector-borne diseases such as dengue and malaria. the hexameric storage protein, hexamerin-1.2, of the mosquito aedes atropalpus is female-specific and uniquely expressed in the fat body of fou ...201223241066
integration of botanical and bacterial insecticide against aedes aegypti and anopheles stephensi.the present study evaluated the orthosiphon thymiflorus leaf extract and the bacterial insecticide spinosad, testing the first to fourth instars larvae and pupae of two important vector mosquitoes, viz., aedes aegypti, anopheles stephensi. the fresh leaves of o. thymiflorus were washed thoroughly in tap water and shade-dried at room temperature (28 ± 2 °c) for 5 to 8 days. the air-dried materials were powdered separately using a commercial electrical blender. from the plants, 500 g powder was ma ...201323242266
[mayaro: a re-emerging arbovirus in venezuela and latin america].mayaro virus produces nonspecific, sublethal disease symptoms, often confused with dengue, but with symptoms of arthalgias that can cause incapacitating disability. outbreaks have been localized and sporadic in the pan-amazonia forest since its first isolation in 1954 (trinidad and tobago). the literature available is scarce, diverse and dispersed. mayaro virus is an alphavirus, phylogenetically related to the semliki forest antigenic complex. una and mayaro viruses are the only viruses of this ...201223242303
replacement of conserved or variable sequences of the mosquito-borne dengue virus 3' utr with homologous sequences from modoc virus does not change infectivity for mosquitoes.the genus flavivirus includes both vector-borne and no known vector (nkv) species, but the molecular determinants of transmission mode are not known. conserved sequence differences between the two groups occur in 5' and 3' utrs. to investigate the impact of these differences on transmission, chimeric genomes were generated, in which utrs, utrs+capsid, or the upper 3' utr stem-loop of mosquito-borne dengue virus (denv) were replaced with homologous regions from nkv modoc virus (modv); the conserv ...201223255623
ovicidal, repellent, adulticidal and field evaluations of plant extract against dengue, malaria and filarial vectors.mosquitoes are insect vectors responsible for the transmission of parasitic and viral infections to millions of people worldwide, with substantial morbidity and mortality. infections transmitted by mosquitoes include malaria, yellow fever, chikungunya, filariasis and other arboviruses. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. the adulticidal activities of crude hexane, benzene, ethyl acetate, acetone and methanol leaf extracts of aca ...201323271569
identifying the effective concentration for spatial repellency of the dengue vector aedes aegypti.current efforts are underway to quantify the chemical concentration in a treated air space that elicits a spatial repellent (deterrent) response in a vector population. such information will facilitate identifying the optimum active ingredient (ai) dosage and intervention coverage important for the development of spatial repellent tools--one of several novel strategies being evaluated for vector-borne disease control. this study reports initial findings from air sampling experiments conducted un ...201223273133
house-to-house human movement drives dengue virus transmission.dengue is a mosquito-borne disease of growing global health importance. prevention efforts focus on mosquito control, with limited success. new insights into the spatiotemporal drivers of dengue dynamics are needed to design improved disease-prevention strategies. given the restricted range of movement of the primary mosquito vector, aedes aegypti, local human movements may be an important driver of dengue virus (denv) amplification and spread. using contact-site cluster investigations in a case ...201223277539
spatial and temporal patterns of abundance of aedes aegypti l. (stegomyia aegypti) and aedes albopictus (skuse) [stegomyia albopictus (skuse)] in southern florida.invasion by mosquito vectors of disease may impact the distribution of resident mosquitoes, resulting in novel patterns of vectors and concomitant risk for disease. one example of such an impact is the invasion by aedes albopictus (skuse) [stegomyia albopictus (skuse)] (diptera: culicidae) of north america and this species' interaction with aedes aegypti l. (stegomyia aegypti l). we hypothesized that ae. aegypti would be found in urban, coastal areas that experience hotter and drier conditions, ...201323278304
genetic variation in the 3' untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in brazil.dengue, a mosquito-borne viral infection caused by one of the four dengue virus (denv) serotypes (denv-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections throughout tropical and subtropical regions of the world. in brazil, the disease has become a major public health problem and the introduction of denv-3 in 2000 in rio de janeiro (rj) was associated with severe dengue epidemics. the potential emergence of strains associated with severe diseas ...201323282086
assessing the feasibility of controlling aedes aegypti with transgenic methods: a model-based evaluation.suppression of dengue and malaria through releases of genetically engineered mosquitoes might soon become feasible. aedes aegypti mosquitoes carrying a conditionally lethal transgene have recently been used to suppress local vector populations in small-scale field releases. prior to releases of transgenic insects on a wider scale, however, most regulatory authorities will require additional evidence that suppression will be effective in natural heterogeneous habitats. we use a spatially explicit ...201223284949
need for an efficient adult trap for the surveillance of dengue vectors.the emergence and re-emergence of arboviral diseases transmitted by aedes aegypti and ae. albopictus continue to be a major threat in the tropics and subtropics. associations between currently used indices and dengue transmission have not been proven to be satisfactorily predictive of dengue epidemics. classical larval indices in dengue surveillance have limited use in assessing transmission risk and are a poor proxy for measuring adult emergence. besides, collection of larval indices is labour ...023287120
evaluation of melaleuca cajuputi (family: myrtaceae) essential oil in aerosol spray cans against dengue vectors in low cost housing flats.melaleuca cajuputi essential oil in aerosol spray was evaluated against the dengue vectors aedes aegypti and ae. albopictus at low cost housing flats in section 10, setapak, kuala lumpur, malaysia.201223293776
the native wolbachia symbionts limit transmission of dengue virus in aedes albopictus.the chikungunya (chik) outbreak that struck la reunion island in 2005 was preceded by few human cases of dengue (den), but which surprisingly did not lead to an epidemic as might have been expected in a non-immune population. both arboviral diseases are transmitted to humans by two main mosquito species, aedes aegypti and aedes albopictus. in the absence of the former, ae. albopictus was the only species responsible for viral transmission on la reunion island. this mosquito is naturally super-in ...201223301109
dengue vector control and surveillance during a major outbreak in a coastal red sea area in sudan.an unprecedented dengue outbreak occurred in 2010 in port sudan city, sudan. dengue incidence was 94 cases per 10 000 observed over 17 epidemiological weeks (total cases = 3 765). we report here the impact of the vector control response plan to the outbreak, which mainly entailed house inspection and insecticide space spraying. in total 3 048 houses were inspected during vector surveillance and 19 794 larvae and 3 240 pupae of aedes aegypti were collected. entomological indices decreased during ...201223301396
frequency and clinical features of dengue infection in a schoolchildren cohort from medellin, colombia.to determine the incidence of dengue infection, we established active surveillance of febrile episodes in a cohort of schoolchildren from three schools in medellin, colombia. we followed a cohort of 2,379 schoolchildren in 2010 and followed 1,840 of these children the following year. during the follow-up time, 264 schoolchildren displayed 297 febrile episodes; of these, 23 episodes (7.7%) were caused by acute dengue infection. all four dengue serotypes were found, and all of the cases were mild. ...201223304167
mosquito attractant blends to trap host seeking aedes aegypti.aedes aegypti is the key vector of three important arboviral diseases -dengue, yellow fever and chikungunya. to identify volatile chemicals which could be used in odour based traps for aedes mosquito surveillance, a few synthetic compounds and compound blends have been evaluated in an indigenously designed olfactometer. a total of 24 compounds and seven compound blends were screened against unfed adult female ae. aegypti mosquitoes for attraction and compared with control group. the attractancy ...201323306388
field trial on a novel control method for the dengue vector, aedes aegypti by the systematic use of olyset® net and pyriproxyfen in southern vietnam.jars, tanks, and drums provide favorable rearing/breeding sites for aedes aegypti in vietnam. however, the use of insecticides to control mosquitoes at such breeding sites has not been approved in vietnam since they are also often sources of drinking water, making larval vector control difficult. mosquito nets pre-treated with long-lasting insecticide treated nets (llitns) form an effective measure for malaria control. we examined changes in the abundance of immature aedes aegypti to evaluate th ...201323312018
estimating dengue vector abundance in the wet and dry season: implications for targeted vector control in urban and peri-urban asia.research has shown that the classical stegomyia indices (or "larval indices") of the dengue vector aedes aegypti reflect the absence or presence of the vector but do not provide accurate measures of adult mosquito density. in contrast, pupal indices as collected in pupal productivity surveys are a much better proxy indicator for adult vector abundance. however, it is unknown when it is most optimal to conduct pupal productivity surveys, in the wet or in the dry season or in both, to inform contr ...023318235
community-based control of aedes aegypti by adoption of eco-health methods in chennai city, india.dengue is highly endemic in chennai city, south india, in spite of continuous vector control efforts. this intervention study was aimed at establishing the efficacy as well as the favouring and limiting factors relating to a community-based environmental intervention package to control the dengue vector aedes aegypti.023318241
the efficacy of a combined larvicide-adulticide in ultralow volume and fumigant canister formulations in controlling the dengue vector aedes aegypti (diptera: culicidae) in northwest of argentina.the efficacy of an ultralow volume formulation (ulv) and fumigant canister, containing both permethrin and pyriproxyfen, was compared with that of standard permethrin applications in a field assay conducted in banda del río salí, tucumán (north-western argentina). five treatment areas were established: first area was sprayed with a ulv formulation of 10 % permethrin, a second area was treated using a fumigant canister containing 10 % permethrin and 3 % pyriproxyfen, the third area was sprayed wi ...201323322325
identification of new protein interactions between dengue fever virus and its hosts, human and mosquito.the four divergent serotypes of dengue virus are the causative agents of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. about two-fifths of the world's population live in areas where dengue is prevalent, and thousands of deaths are caused by the viruses every year. dengue virus is transmitted from one person to another primarily by the yellow fever mosquito, aedes aegypti. recent studies have begun to define how the dengue viral proteins interact with host proteins to mediate ...201323326450
population genetic structure of aedes (stegomyia) aegypti (l.) at a micro-spatial scale in thailand: implications for a dengue suppression strategy.the genetic population structure of aedes (stegomyia) aegypti (l.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. the aim of the program is to release and establish mosquito populations with impaired virus transmission capabilities. to model effects of the release and devise protocols for its implementation, information about the genetic structure of populations at a range of spatial scales is required.201323326609
transgene-mediated suppression of the rna interference pathway in aedes aegypti interferes with gene silencing and enhances sindbis virus and dengue virus type 2 replication.rna interference (rnai) is the major innate antiviral pathway in aedes aegypti that responds to replicating arboviruses such as dengue virus (denv) and sindbis virus (sinv). on the one hand, the mosquito's rnai machinery is capable of completely eliminating denv2 from ae. aegypti. on the other, transient silencing of key genes of the rnai pathway increases replication of sinv and denv2, allowing the viruses to temporally overcome dose-dependent midgut infection and midgut escape barriers (meb) m ...023331493
glytube: a conical tube and parafilm m-based method as a simplified device to artificially blood-feed the dengue vector mosquito, aedes aegypti.aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. these feeders are also the best method for experimental oral infection of ae. aegypti with dengue viruses. however, most of them are expensive or laborious to construct. based on principle of rutledge-type feeder, a conventional conical tube, glycerol and parafilm-m were used to develop a simpl ...201323342010
spatial patterns of high aedes aegypti oviposition activity in northwestern argentina.in argentina, dengue has affected mainly the northern provinces, including salta. the objective of this study was to analyze the spatial patterns of high aedes aegypti oviposition activity in san ramón de la nueva orán, northwestern argentina. the location of clusters as hot spot areas should help control programs to identify priority areas and allocate their resources more effectively.201323349813
field cage studies and progressive evaluation of genetically-engineered mosquitoes.a genetically-engineered strain of the dengue mosquito vector aedes aegypti, designated ox3604c, was evaluated in large outdoor cage trials for its potential to improve dengue prevention efforts by inducing population suppression. ox3604c is engineered with a repressible genetic construct that causes a female-specific flightless phenotype. wild-type females that mate with homozygous ox3604c males will not produce reproductive female offspring. weekly introductions of ox3604c males eliminated all ...201323350003
bacillus thuringiensis var. israelensis misting for control of aedes in cryptic ground containers in north queensland, australia.in australia, dengue is not endemic, although the vector mosquito aedes aegypti is established in far north queensland (fnq). aedes albopictus has recently invaded the torres strait region, but is not established on mainland australia. to maintain dengue-free, public health departments in fnq closely monitor introduced dengue infections and confine outbreaks through rigorous vector control responses. to safeguard mainland australia from ae. albopictus establishment, pre-emptive strategies are re ...201323358637
evolution of resistance to satyrization through reproductive character displacement in populations of invasive dengue vectors.recently, the highly invasive asian tiger mosquito, aedes albopictus, rapidly displaced resident populations of the yellow fever mosquito, aedes aegypti in the southeastern united states and in bermuda. although multiple mechanisms of competitive displacement have been hypothesized, recent evidence of cross-insemination between these species in nature and the sterilizing effects of male accessory gland products asymmetrically favoring a. albopictus in interspecific matings support a role for sat ...201323359710
[an imported dengue fever case in turkey and review of the literature].dengue fever is an acute viral disease that can affect all age groups in tropical and subtropical countries. the predominant vectors are the mosquitoes namely aedes aegypti and a.albopictus. although there have been no case reports in turkey due to df, there is seroepidemiological evidence indicating the presence of dengue virus (denv) in turkey. in this case report we presented an imported dengue fever case. the patient was 40 years old, previously healthy male, switzerland citizen. he had immi ...201323390916
transinfected wolbachia have minimal effects on male reproductive success in aedes aegypti.wolbachia are maternally inherited endosymbiotic bacteria that manipulate the reproductive success of their insect hosts. uninfected females that mate with wolbachia infected males do not reproduce due to cytoplasmic incompatibility (ci). ci results in the increased frequency of wolbachia-infected individuals in populations. recently, two wolbachia strains, the benign wmel and virulent wmelpop have been artificially transinfected into the primary vector of dengue virus, the mosquito ae. aegypti ...201323399027
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