Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [association between dengue incidence, rainfall and larval density of aedes aegypti, in the state of goiás]. | the larval density of aedes aegypti fluctuates according to seasonal climatic changes. it rises in seasons with higher rainfall, according to the number of potential breeding sites available, thereby predisposing towards increased incidence of dengue. this study aimed to show the association between dengue cases, rainfall and the building infestation rate. | 2010 | 20464144 |
| screening of mosquitoes using sybr green i-based real-time rt-pcr with group-specific primers for detection of flaviviruses and alphaviruses in taiwan. | surveillance for infectious agents carried by mosquitoes is important for predicting the risk of vector-borne infectious diseases. in this study, a method was established to mass-screen mosquitoes for viral infections. the assay detected the viral load of 4 dengue virus (denv) serotypes (denv-1, denv-2, denv-3, and denv-4), the japanese encephalitis virus (jev), the sindbis virus and the chikungunya virus at 1pfu/ml (determined by real-time rt-pcr) in 36.64-43.45 cycles. this method was applied ... | 2010 | 20471427 |
| [geospatial distribution and detection of dengue virus in aedes (stegomyia) aegypti mosquitos in ciudad juárez, chihuahua, mexico]. | to determine the distribution of aedes aegypti in ciudad juárez, mexico and evaluate it as a carrier of the dengue virus. | 2010 | 20485869 |
| a large scale laboratory cage trial of aedes densonucleosis virus (aednv). | aedes aegypti (l.) (diptera: culicidae) the primary vector of dengue viruses (denv1-4), oviposit in and around human dwellings, including sites difficult to locate, making control of this mosquito challenging. we explored the efficacy and sustainability of aedes densonucleosis virus (aednv) as a biocontrol agent for ae. aegypti in and among oviposition sites in large laboratory cages (> 92 m3) as a prelude to field trials. select cages were seeded with aednv in a single oviposition site (ops) wi ... | 2010 | 20496587 |
| dengue virus inhibits immune responses in aedes aegypti cells. | the ability of many viruses to manipulate the host antiviral immune response often results in complex host-pathogen interactions. in order to study the interaction of dengue virus (denv) with the aedes aegypti immune response, we have characterized the denv infection-responsive transcriptome of the immune-competent a. aegypti cell line aag2. as in mosquitoes, denv infection transcriptionally activated the cell line toll pathway and a variety of cellular physiological systems. most notably, howev ... | 2010 | 20502529 |
| dengue virus 3 genotype i in aedes aegypti mosquitoes and eggs, brazil, 2005-2006. | dengue virus type 3 genotype i was detected in brazil during epidemics in 2002-2004. to confirm this finding, we identified this virus genotype in naturally infected field-caught aedes aegypti mosquitoes and eggs. results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance. | 2010 | 20507754 |
| transcriptomics and disease vector control. | next-generation sequencing can be used to compare transcriptomes under different conditions. a study in bmc genomics applies this approach to investigating the effects of exposure to a range of xenobiotics on changes in gene expression in the larvae of aedes aegypti, the mosquito vector of dengue fever. | 2010 | 20525113 |
| patient-based dengue virus surveillance in aedes aegypti from recife, brazil. | dengue is currently one of the most important arthropod-borne diseases and may be caused by four different dengue virus serotypes (denv-1 to denv-4), transmitted mainly by aedes aegypti (diptera: culicidae) mosquitoes. with the lack of a dengue vaccine, vector control strategies constitute a crucial mode to prevent or reduce disease transmission. in this context, denv detection in natural ae. aegypti populations may serve as a potential additional tool for early prediction systems of dengue outb ... | 2010 | 20539043 |
| beyond the 'back yard': lay knowledge about aedes aegypti in northern australia and its implications for policy and practice. | controlling dengue fever in australia and internationally, relies heavily upon the actions of residents as well as community education and awareness of the risks. although it has been well established in medical anthropology that the success of health interventions is highly dependent upon a clear grasp of lay knowledge of disease, limited attention has been given to lay understandings of dengue fever and its vectors in the extant literature. we begin addressing this hiatus through an examinatio ... | 2010 | 20540930 |
| the effect of oral anthelmintics on the survivorship and re-feeding frequency of anthropophilic mosquito disease vectors. | in the tropics, there is substantial temporal and spatial overlap of diseases propagated by anthropophilic mosquito vectors (such as malaria and dengue) and human helminth diseases (such as onchocerciasis and lymphatic filariasis) that are treated though mass drug administrations (mda). this overlap will result in mosquito vectors imbibing significant quantities of these drugs when they blood feed on humans. since many anthelmintic drugs have broad anti-invertebrate effects, the possibility of c ... | 2010 | 20540931 |
| lagged temperature effect with mosquito transmission potential explains dengue variability in southern taiwan: insights from a statistical analysis. | the purpose of this study was to link meteorological factors and mosquito (aedes aegypti) abundance to examine the potential effects of climate variations on patterns of dengue epidemiology in taiwan during 2001-2008. spearman's rank correlation tests with and without time-lag were performed to investigate the overall correlation between dengue incidence rates and meteorological variables (i.e., minimum, mean, and maximum temperatures, relative humidity (rh), and rainfall) and percentage breteau ... | 2010 | 20542536 |
| [bilateral optic neuritis after dengue viral infection: case report]. | dengue fever is a well known viral infection transmitted through the bite of an infected aedes aegypti and aedes albopictus mosquito. its geographic distribution covers over 100 countries. clinical presentation is variable and depends upon the age and immunological status of the patient. it can be classified as asymptomatic illness, dengue fever, dengue hemorrhagic or dengue shock syndrome. ocular manifestations of dengue have been described, however there are few reports on neurological involve ... | 2012 | 20549049 |
| larvicidal potential of ethanolic extracts of dried fruits of three species of peppercorns against different instars of an indian strain of dengue fever mosquito, aedes aegypti l. (diptera: culicidae). | larvicidal bioassay was carried out in the laboratory to assess the potential of ethanolic extracts of dried fruits of three species of peppercorns: long pepper, piper longum l., black pepper, piper nigrum, and white pepper, piper nigrum against the different instars of field-collected indian strain of dengue fever mosquito (aedes aegypti l.). the investigations established the larvicidal potential of all the varieties of pepper fruits against ae. aegypti. against early fourth instar, the ethano ... | 2010 | 20549234 |
| larvicidal activity of para-benzoquinones. | plant products may be alternative sources of mosquito larval control agents, since they constitute a rich source of bioactive compounds that are biodegradable into nontoxic products. it has been reported that quinones and derivatives present toxic activity against mosquito larvae aedes aegypti. therefore, these facts led us to investigate the larvicidal potential of six structurally related para-benzoquinones against a. aegypti l. (culicidae) larvae, the vector of dengue fever. all the para-benz ... | 2010 | 20549237 |
| response of the mosquito protein interaction network to dengue infection. | two fifths of the world's population is at risk from dengue. the absence of effective drugs and vaccines leaves vector control as the primary intervention tool. understanding dengue virus (denv) host interactions is essential for the development of novel control strategies. the availability of genome sequences for both human and mosquito host greatly facilitates genome-wide studies of denv-host interactions. | 2010 | 20553610 |
| residual insecticidal activity of long-lasting deltamethrin-treated curtains after 1 year of household use for dengue control. | to evaluate the residual insecticidal activity of the permanet(®) curtains on aedes aegypti after 1 year of use in thai households and to assess the influence of sun and dust exposure, washing practices and detergent use. | 2010 | 20561312 |
| categorization of potential breeding sites of dengue vectors in johor, malaysia. | this cross-sectional study was to compare and categorize potential breeding sites of dengue vectors, aedes aegypti and aedes albopictus at three different places, namely, an urban (taman permas jaya, johor bahru, johor), a suburban (kg. melayu gelang patah, johor bahru, johor) and a rural (felda simpang waha, kota tinggi, johor) habitats in malaysia. larval surveys were conducted once in every two months at each habitat over a period of 11 months from august 2000 until june 2001. there was a sig ... | 2010 | 20562811 |
| dengue-2-virus-interacting polypeptides involved in mosquito cell infection. | for the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus-host interactions, is essential. we performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of a7 (aedes aegypti) cells, c6/36 (aedes albopictus) cells and the midgut brush border membrane fraction of ae. aegypti mosquito that bind to dengue-2 virus. actin, atp synthase β subunit, hsc 70, orisis, prohi ... | 2010 | 20571839 |
| differential gene expression from midguts of refractory and susceptible lines of the mosquito, aedes aegypti, infected with dengue-2 virus. | suppressive subtractive hybridization was used to evaluate the differential expression of midgut genes of feral populations of aedes aegypti (diptera: culicidae) from colombia that are naturally refractory or susceptible to dengue-2 virus infection. a total of 165 differentially expressed sequence tags (ests) were identified in the subtracted libraries. the analysis showed a higher number of differentially expressed genes in the susceptible ae. aegypti individuals than the refractory mosquitoes. ... | 2010 | 20572793 |
| effect of crude extract of solanum xanthocarpum against snails and mosquito larvae. | the ethanolic crude extract from solanum xanthocarpum was investigated for its molluscicidal activity against biomphalaria glabrata, the snail vector of schistosoma mansoni, and indoplanorbis exustus, the snail vector of intestinal echinostomiasis and schistosoma spindale, together with the larvicidal activity against the larvae of aedes aegypti, mosquito vector of dengue hemorrhagic fever and culex quinquefasciatus, the mosquito vector of urban bancroftian filariasis. the bioassays were carried ... | 2010 | 20578514 |
| incubation periods of yellow fever virus. | yellow fever virus is a global health threat due to its endemicity in parts of africa and south america where human infections occur in residents and travelers. to understand yellow fever dynamics, it is critical to characterize the incubation periods of the virus in vector mosquitoes and humans. here, we compare four statistical models of the yellow fever incubation periods fitted with historical data. the extrinsic incubation period in the urban vector aedes aegypti was best characterized with ... | 2010 | 20595499 |
| evaluation of an oviposition-stimulating kairomone for the yellow fever mosquito, aedes aegypti, in recife, brazil. | a synthetic mixture of an oviposition-stimulating kairomone for the yellow fever mosquito, aedes aegypti, comprising of 83% tetradecanoic acid, 16% nonanoic acid and 1% tetradecanoic acid methyl ester (ntt, in short) was tested in a dengue endemic area in recife, brazil. gravid female mosquitoes confined to a cage under semi-field conditions deposited significantly higher numbers of eggs in traps baited with ntt at doses ranging from 0.6 to 600 ng/microl than in control (water) traps. when teste ... | 2010 | 20618668 |
| genetic relationships among populations of aedes aegypti from uruguay and northeastern argentina inferred from issr-pcr data. | aedes aegypti (l.) (diptera: culicidae), the main vector of yellow fever and dengue viruses, was eradicated from argentina between 1955 and 1963, but reinvaded the country in 1986. in uruguay, the species was reintroduced in 1997. in this study we used highly polymorphic inter-simple sequence repeats (issr) markers to analyse the genetic structure of ae. aegypti populations from uruguay and northeastern argentina to identify possible colonization patterns of the vector. overall genetic different ... | 2010 | 20626627 |
| the development of predictive tools for pre-emptive dengue vector control: a study of aedes aegypti abundance and meteorological variables in north queensland, australia. | to describe the meteorological influences on adult dengue vector abundance in australia for the development of predictive models to trigger pre-emptive control operation. | 2010 | 20636303 |
| dengue hemorrhagic fever and shock syndromes. | to provide a comprehensive review of dengue, with an emphasis on clinical syndromes, classification, diagnosis, and management, and to outline relevant aspects of epidemiology, immunopathogenesis, and prevention strategies. dengue, a leading cause of childhood mortality in asia and south america, is the most rapidly spreading and important arboviral disease in the world and has a geographic distribution of > 100 countries. | 2011 | 20639791 |
| a virulent wolbachia infection decreases the viability of the dengue vector aedes aegypti during periods of embryonic quiescence. | a new approach for dengue control has been proposed that relies on life-shortening strains of the obligate intracellular bacterium wolbachia pipientis to modify mosquito population age structure and reduce pathogen transmission. previously we reported the stable transinfection of the major dengue vector aedes aegypti with a life-shortening wolbachia strain (wmelpop-cla) from the vinegar fly drosophila melanogaster. here, we report a further characterization of the phenotypic effects of this viru ... | 2010 | 20644622 |
| wolbachia pipientis: an expanding bag of tricks to explore for disease control. | wolbachia pipientis are maternally inherited, endosymbiotic bacteria that are widespread among insects. two recent studies have demonstrated that wolbachia inhibits the ability of medically significant pathogens, including filarial nematodes, dengue virus and plasmodium to form infections in the mosquito vector, aedes aegypti. we highlight the added value of these traits for wolbachia based biocontrol strategies and evaluate the evidence for the idea that the insect immune response is responsibl ... | 2010 | 20647151 |
| an updated checklist of species of aedes and verrallina of northeastern india. | a mosquito faunistic survey conducted during 1999-2005 in 7 different states of the northeastern (ne) region of india revealed that the region is very rich in mosquito fauna, particularly of the genus aedes. from the present survey and earlier published reports, a total of 48 species of aedes and a related genus verrallina were recorded. the state of assam contributed the highest number of species compared to the other 6 states, namely arunachal pradesh, manipur, meghalaya, mizoram, nagaland, an ... | 2010 | 20649122 |
| identification and characterization of prohibitin as a receptor protein mediating denv-2 entry into insect cells. | dengue is transmitted primarily by mosquitoes of the aedes genus. despite a number of studies, no insect dengue virus receptor protein has been clearly identified and characterized. using a number of separation methodologies and virus overlay protein binding assays we identified a 35kda protein that segregated with susceptibility to dengue serotype 2 (denv-2) infection in two mosquito species and two mosquito cell lines. mass spectroscopy identified the protein to be prohibitin, a strongly conse ... | 2010 | 20674955 |
| toward specific detection of dengue virus serotypes using a novel modular biosensor. | arthropod-borne diseases affect a significant portion of the world's population. dengue fever, a viral disease carried by the aedes aegypti mosquito, is one of the most wide-spread, with many fatalities evident each year. to date, dengue viral diagnostic technologies have been too complex, time-consuming and expensive to be widely deployed, particularly in developing countries where the disease is most prevalent. here we demonstrate a modular biosensor that is able to rapidly identify sequences ... | 2010 | 20692150 |
| antioxidant, larvicidal and antiacetylcholinesterase activities of cashew nut shell liquid constituents. | anacardic acid, cardanol and cardol, the main constituents of natural cashew nut shell liquid (cnsl), were obtained by solvent extraction and assayed for antioxidant, larvicidal and antiacetylcholinesterase activity. their relative percent composition was obtained by hplc analysis. antioxidant activity was assessed using the dpph and abts(+) tests, which showed cardanol as the most active, followed by cardol and anacardic acid. the three cnsl components demonstrated good larvicidal activity agai ... | 2011 | 20707981 |
| domain-iii fg loop of the dengue virus type 2 envelope protein is important for infection of mammalian cells and aedes aegypti mosquitoes. | the fg extended loop in domain iii of the dengue virus type 2 (denv2) envelope protein is postulated to be a molecular determinant for host cell infectivity. to determine the contribution of the fg loop to virus infectivity, an infectious cdna clone of denv2 was manipulated by deleting amino acids in the loop (vepgδ) to mimic tick-borne flaviviruses or by substituting these aas with rgd or rgdk/s to mimic motifs present in other mosquito-borne flaviviruses. we found the fg loop to be dispensable ... | 2010 | 20708768 |
| transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, aedes aegypti. | controlled sex-, stage- and tissue-specific expression of antipathogen effector molecules is important for genetic engineering strategies to control mosquito-borne diseases. adult female salivary glands are involved in pathogen transmission to human hosts and are target sites for expression of antipathogen effector molecules. the aedes aegypti 30k a and 30k b genes are expressed exclusively in adult female salivary glands and are transcribed divergently from start sites separated by 263 nucleoti ... | 2010 | 20738425 |
| the essential oil of brazilian pepper, schinus terebinthifolia raddi in larval control of stegomyia aegypti (linnaeus, 1762). | abstract: | 2010 | 20799936 |
| new protective battle-dress impregnated against mosquito vector bites. | mixing repellent and organophosphate (op) insecticides to better control pyrethroid resistant mosquito vectors is a promising strategy developed for bed net impregnation. here, we investigated the opportunity to adapt this strategy to personal protection in the form of impregnated clothes. | 2010 | 20809969 |
| a simple colorimetric assay for specific detection of glutathione-s transferase activity associated with ddt resistance in mosquitoes. | insecticide-based methods represent the most effective means of blocking the transmission of vector borne diseases. however, insecticide resistance poses a serious threat and there is a need for tools, such as diagnostic tests for resistance detection, that will improve the sustainability of control interventions. the development of such tools for metabolism-based resistance in mosquito vectors lags behind those for target site resistance mutations. | 2010 | 20824165 |
| cultural factors in aedes aegypti and dengue control in latin america: a case study from the dominican republic. | dengue and aedes aegypti infestation in latin america pose substantial health risks. total reliance on chemical interventions is no longer feasible and, increasingly, programs of control will need to rely on education, behavior change and community participation. findings from applied behavioral research in the dominican republic conducted from july through september of 1987 are discussed in the light of cultural constraints on intervention efforts. perception of risk, the choices for curative c ... | 1989 | 20840930 |
| field evaluation of pyriproxyfen and spinosad mixture for the control of insecticide resistant aedes aegypti in martinique (french west indies). | the resistance of ae. aegypti to insecticides is already widespread and continues to develop. it represents a serious problem for programmes aimed at the control and prevention of dengue in tropical countries. in the light of this problem measures to control ae. aegypti are being orientated towards how best to use existing insecticides, notably by combining those that have different modes of action. | 2010 | 20843383 |
| aedes aegypti: an emerging model for vector mosquito development. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects. targets of particular interest include genes that regulate development. however, although ... | 2010 | 20889691 |
| culturing and egg collection of aedes aegypti. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. this protocol describes me ... | 2010 | 20889704 |
| fixation and preparation of developing tissues from aedes aegypti. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. this protocol describes a ... | 2010 | 20889705 |
| whole-mount in situ hybridization for analysis of gene expression during aedes aegypti development. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. this protocol for whole-mo ... | 2010 | 20889706 |
| immunohistochemical analysis of protein expression during aedes aegypti development. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and gene products that regulate development are of particular interest. this protocol for ... | 2010 | 20889707 |
| functional analysis of genes in aedes aegypti embryos. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. in recent years, rna inter ... | 2010 | 20889708 |
| evaluation of a rapid water-surface sweeping method to accurately estimate numbers of aedes aegypti (diptera: culicidae) late larval stages in large water-storage containers: comparison with pupal estimates. | since the methodologies used to calculate stegomyia indices have been shown to be inadequate for assessing the risk of dengue virus transmission and targeting aedes aegypti control strategies, new surveillance methods are needed. | 2010 | 20890574 |
| aegepuci: a database of gene expression in the dengue vector mosquito, aedes aegypti. | aedes aegypti is the principal vector of dengue and yellow fever viruses. the availability of the sequenced and annotated genome enables genome-wide analyses of gene expression in this mosquito. the large amount of data resulting from these analyses requires efficient cataloguing before it becomes useful as the basis for new insights into gene expression patterns and studies of the underlying molecular mechanisms for generating these patterns. | 2010 | 20920356 |
| prevention of dengue outbreaks through aedes aegypti oviposition activity forecasting method. | dengue has affected the north provinces of argentina, mainly salta province. the 2009 outbreak, with 5 deaths and >27,000 infected, was the most important, and the first to extend into the central area of the country. this article includes research on seasonal aedes aegypti abundance variation in orán city (salta province), and determination of the date of mosquito population increase and an estimation of the date of maximum rate of increase as well as the intrinsic rate of natural increase (r), ... | 2011 | 20925528 |
| effects of inbreeding and genetic modification on aedes aegypti larval competition and adult energy reserves. | genetic modification of mosquitoes offers a promising strategy for the prevention and control of mosquito-borne diseases. for such a strategy to be effective, it is critically important that engineered strains are competitive enough to serve their intended function in population replacement or reduction of wild mosquitoes in nature. thus far, fitness evaluations of genetically modified strains have not addressed the effects of competition among the aquatic stages and its consequences for adult f ... | 2010 | 20925917 |
| genome-based microsatellite development in the culex pipiens complex and comparative microsatellite frequency with aedes aegypti and anopheles gambiae. | mosquitoes in the culex pipiens complex are among the most medically important vectors for human disease worldwide and include major vectors for lymphatic filariasis and west nile virus transmission. however, detailed genetic studies in the complex are limited by the number of genetic markers available. here, we describe methods for the rapid and efficient identification and development of single locus, highly polymorphic microsatellite markers for cx. pipiens complex mosquitoes via in silico sc ... | 2010 | 20927334 |
| acute and sub-acute toxicity of an insect pheromone, n-heneicosane and combination with insect growth regulator, diflubenzuron, for establishing no observed adverse effect level (noael). | aedes aegypti mosquito is one of the most notorious vectors of dangerous diseases like dengue hemorrhagic fever and chikangunya. one method of control of the vectors is by the use of semiochemicals or pheromones. the pheromone n-heneicosane (c21) has been proved to be effective in attracting the female aedes aegypti to lay eggs in the treated water and the growth of the larva is controlled by insect growth regulator diflubenzuron (db). this study was planned to assess the safety of c21 alone and ... | 2010 | 20929058 |
| evidence of co-infection of chikungunya and densonucleosis viruses in c6/36 cell lines and laboratory infected aedes aegypti (l.) mosquitoes. | abstract: | 2010 | 20939884 |
| differential protein modulation in midguts of aedes aegypti infected with chikungunya and dengue 2 viruses. | arthropod borne virus infections cause several emerging and resurgent infectious diseases. among the diseases caused by arboviruses, dengue and chikungunya are responsible for a high rate of severe human diseases worldwide. the midgut of mosquitoes is the first barrier for pathogen transmission and is a target organ where arboviruses must replicate prior to infecting other organs. a proteomic approach was undertaken to characterize the key virus/vector interactions and host protein modifications ... | 2010 | 20957153 |
| dengue vector surveillance in insular settlements of pulau ketam, selangor, malaysia. | a year-long ovitrap surveillance was conducted between november 2007 and october 2008 in two insular settlements (kampung pulau ketam and kampung sungai lima) within the malaysian island of pulau ketam. eighty standard ovitraps were placed indoors and outdoors of randomly selected houses/locations. results demonstrated an endemic baseline aedes population throughout the year without weekly large fluctuations. kampung pulau ketam has high aedes aegypti and aedes albopictus population, but only ae ... | 2010 | 20962714 |
| wolbachia-mediated resistance to dengue virus infection and death at the cellular level. | dengue is currently the most important arthropod-borne viral disease of humans. recent work has shown dengue virus displays limited replication in its primary vector, the mosquito aedes aegypti, when the insect harbors the endosymbiotic bacterium wolbachia pipientis. wolbachia-mediated inhibition of virus replication may lead to novel methods of arboviral control, yet the functional and cellular mechanisms that underpin it are unknown. | 2010 | 20976219 |
| impact of parthenium hysterophorus leaf extracts on the fecundity, fertility and behavioural response of aedes aegypti l. | different extracts of 1,000 ppm were prepared from the leaves of parthenium hysterophorus using acetone, benzene, petroleum ether, diethyl ether and hexane as the solvents. the efficacy of each extract was assessed against dengue fever vector, aedes aegypti by evaluating the variations in fecundity, fertility and behavioural response of the female adults. the leaf extracts could cause 70-100% repellency in the oviposition behaviour of the adults. the diethyl ether extract was found to be the mos ... | 2011 | 20978787 |
| mosquito vector biology and control in latin america--a 20th symposium. | the 20th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 76th annual meeting in lexington, ky, in march 2010. the principal objective, as for the previous 19 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 40 presentations that were given orally in spanish or presented as posters by participants ... | 2010 | 21033058 |
| serological investigations of flavivirus prevalence in khammouane province, lao people's democratic republic, 2007-2008. | a large-scale cross-sectional seroprevalence study of dengue (den) and japanese encephalitis (je) was conducted in khammouane province, lao pdr, as part of the initial baseline health impact assessment of the nam theun 2 hydroelectric dam construction project. health surveys were performed between may 2007 and february 2008 with serum samples collected from healthy individuals involved in the resettlement program of 16 villages (total surveyed population 4,369). hemagglutination inhibition assay ... | 2010 | 21036856 |
| comparison of dengue virus type 2-specific small rnas from rna interference-competent and -incompetent mosquito cells. | the exogenous rna interference (rnai) pathway is an important antiviral defense against arboviruses in mosquitoes, and virus-specific small interfering (si)rnas are key components of this pathway. understanding the biogenesis of sirnas in mosquitoes could have important ramifications in using rnai to control arbovirus transmission. using deep sequencing technology, we characterized dengue virus type 2 (denv2)-specific small rnas produced during infection of aedes aegypti mosquitoes and a. aegypt ... | 2010 | 21049014 |
| survival of betta splendens fish (regan, 1910) in domestic water containers and its effectiveness in controlling aedes aegypti larvae (linnaeus, 1762) in northeast brazil. | objective: in northeast brazil, large domestic containers used to store water are important breeding sites of aedes aegypti, the main vector of dengue fever. the objective of this study was to estimate the survival of betta splendens (perciformes: osphronemidae) fish in domestic containers in fortaleza (ceará state), as well as its effectiveness in the control of premature a. aegypti stages. methods: the use of b. splendens was compared to bacillus thuringiensis israelensis (bti) in domestic con ... | 2010 | 21054694 |
| heritability and adaptive phenotypic plasticity of adult body size in the mosquito aedes aegypti with implications for dengue vector competence. | adaptive phenotypic plasticity is particularly important to organisms with developmental cycles that undergo ontogenetic niche shifts that differentially subject individual life stages to heterogeneous and often stressful environmental conditions. the yellow fever and dengue fever vector mosquito, aedes aegypti, typically breeds in small water-filled containers that expose the developing aquatic larvae to competition for resources with conspecifics and high probabilities for habitat drying. here ... | 2010 | 21070891 |
| geo-database use to promote dengue infection prevention and control. | dengue infection (di) is a major health problem in thailand and is especially prevalent in ubon ratchathani province. the objectives of the project were: (1) to develop a geo-database system for di prevention and control, (2) to perform an aedes aegypti larval vector survey for di prevention and control in ubon ratchathani province, (3) to study the behavior and perceptions regarding di prevention among the target population in ubon ratchathani province. ten villages with high incidences of di o ... | 2010 | 21073058 |
| infection with the wmel and wmelpop strains of wolbachia leads to higher levels of melanization in the hemolymph of drosophila melanogaster, drosophila simulans and aedes aegypti. | introduction of the life-shortening strain of wolbachia pipientis, wmelpop, into the key dengue vector, aedes aegypti, and the anti-pathogen effects in wolbachia-infected hosts highlights the need for more research into its interactions with its original host, drosophila melanogaster, and the novel mosquito host. the visual difference in darkness between the eggs of wmelpop wolbachia-infected and uninfected mosquito hosts after egg deposition led to further investigation into melanization levels ... | 2010 | 21075139 |
| arbovirus detection in insect vectors by rapid, high-throughput pyrosequencing. | despite the global threat caused by arthropod-borne viruses, there is not an efficient method for screening vector populations to detect novel viral sequences. current viral detection and surveillance methods based on culture can be costly and time consuming and are predicated on prior knowledge of the etiologic agent, as they rely on specific oligonucleotide primers or antibodies. therefore, these techniques may be unsuitable for situations when the causative agent of an outbreak is unknown. | 2010 | 21085471 |
| follow up estimation of aedes aegypti entomological parameters and mathematical modellings. | the dengue virus is a vector-borne disease transmitted by mosquito aedes aegypti and the incidence is strongly influenced by temperature and humidity which vary seasonally. to assess the effects of temperature on dengue transmission, mathematical models are developed based on the population dynamics theory. however, depending on the hypotheses of the modelling, different outcomes regarding to the risk of epidemics are obtained. we address this question comparing two simple models supplied with m ... | 2010 | 21093536 |
| best practices in dengue surveillance: a report from the asia-pacific and americas dengue prevention boards. | dengue fever is a virus infection that is spread by the aedes aegypti mosquito and can cause severe disease especially in children. dengue fever is a major problem in tropical and sub-tropical regions of the world. | 2010 | 21103381 |
| microrna mir-275 is indispensable for blood digestion and egg development in the mosquito aedes aegypti. | the mosquito aedes aegypti is the major vector of arboviral diseases, particularly of dengue fever, of which there are more than 100 million cases annually. mosquitoes, such as a. aegypti, serve as vectors for disease pathogens because they require vertebrate blood for their egg production. pathogen transmission is tightly linked to repeated cycles of obligatory blood feeding and egg maturation. thus, the understanding of mechanisms governing egg production is necessary to develop approaches tha ... | 2010 | 21115818 |
| oviposition deterrent activity from the ethanolic extract of pongamia pinnata, coleus forskohlii, and datura stramonium leaves against aedes aegypti and culex quinquefaciatus. | mosquitoes are responsible for spread of many diseases than any other group of arthropods. diseases such as malaria, filariasis, dengue hemorrhagic fever (dhf), and chikunguinya are real threat to mankind. in the present study, ethanolic extracts of leaves of pongamia pinnata, coleus forskohlii, and datura stramonium were evaluated for oviposition deterrent activity against aedes aegypti and culex quinquefasciatus. the oviposition deterrent tests of ethanolic extract of pongamia pinnata, coleus ... | 0 | 21120036 |
| [toxic activity of bacillus thuringiensis isolates to aedes aegypti (l.) (diptera: culicidae) larvae]. | aedes aegypti (l.), the main vector of dengue fever in brazil, has been controlled with the use of massive chemical products, contributing to the development of resistance and decreasing the insect control efficiency. the control of dipterans with bioinsecticides based on bacillus thuringiensis has been satisfactory, due to the production of insecticidal proteins denominated cry (crystal), cyt (cytolytic) toxins and chi (chitinase), and to the synergistic effects among them. the present work aim ... | 2010 | 21120386 |
| screening of dengue virus in field-caught aedes aegypti and aedes albopictus (diptera: culicidae) by one-step sybr green-based reverse transcriptase-polymerase chain reaction assay during 2004-2007 in southern taiwan. | we carried out virological surveillance of dengue virus (denv) in field-caught aedes mosquitoes during 2004-2007 to estimate the monthly prevalence of infected females in dengue high-risk areas of taiwan. a total of 92,892 aedes aegypti (43,133 females and 49,759 males) and 79,315 aedes albopictus (57,319 females and 21,996 males) adults were collected, grouped into 25,654 pools, and processed for virus detection using a one-step sybr green-based real-time reverse transcriptase-polymerase chain ... | 2010 | 21128850 |
| dynamics of the "popcorn" wolbachia infection in outbred aedes aegypti informs prospects for mosquito vector control. | forty percent of the world's population is at risk of contracting dengue virus, which produces dengue fever with a potentially fatal hemorrhagic form. the wmelpop wolbachia infection of drosophila melanogaster reduces life span and interferes with viral transmission when introduced into the mosquito aedes aegypti, the primary vector of dengue virus. wolbachia has been proposed as an agent for preventing transmission of dengue virus. population invasion by wolbachia depends on levels of cytoplasm ... | 2010 | 21135075 |
| chimeric tick-borne encephalitis/dengue virus is attenuated in ixodes scapularis ticks and aedes aegypti mosquitoes. | abstract in an effort to derive an efficacious live attenuated vaccine against tick-borne encephalitis, we generated a chimeric virus bearing the structural protein genes of a far eastern subtype of tick-borne encephalitis virus (tbev) on the genetic background of recombinant dengue 4 (den4) virus. introduction of attenuating mutations into the tbev envelope protein gene, as well as the den4 ns5 protein gene and 3' noncoding region in the chimeric genome, results in decreased neurovirulence and ... | 2010 | 21142950 |
| spinosad as an effective larvicide for control of aedes albopictus and aedes aegypti, vectors of dengue in southern mexico. | field trials were conducted during the wet and dry seasons in periurban and semi-rural cemeteries in southern mexico to determine the efficacy of a suspension concentrate formulation of spinosad (tracer 480sc) on the inhibition of development of aedes albopictus l. and ae. aegypti skuse. for this, oviposition traps were treated with spinosad (1 or 5 mg l(-1)), bacillus thuringiensis israelensis (bti, vectobac 12as), a sustained release formulation of temephos and a water control. | 2010 | 21162151 |
| population genetic structure of aedes aegypti, the principal vector of dengue viruses. | knowledge of vector population genetic structure is critical for vector-borne disease control and prevention strategies. advances in both molecular genotyping technology and theoretical developments have contributed to the growing impact of such approaches on medical entomology. the pattern of genetic structure may affect the design of control strategies in determining appropriate control limits necessary to disrupt pathogen transmission. in this review, we focus on the mosquito aedes aegypti, t ... | 2010 | 21167319 |
| early history of laboratory breeding of aedes aegypti (diptera: culicidae) focusing on the origins and use of selected strains. | the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae), is well recognized for its extensive adaptation to diverse ecological conditions and for genetic variation. recognizing the importance of strain variation of this mosquito, researchers have established a large number of laboratory strains. some of the popular strains have been used for research for years in many laboratories around the world. however, the exact origins of many of these strains are unknown. in this review, public ... | 2010 | 21175042 |
| biochemical evidence of efficacy of potash alum for the control of dengue vector aedes aegypti (linnaeus). | aedes aegypti is the primary vector of dengue, yellow fever and chikungunya in india and other south east asian countries, and novel insecticides for vector control are urgently needed. in the present investigation, efficacy of potash alum, a traditionally known double salt in indian and chinese medicine system, was tested against the larvae of dengue vector, a. aegypti. lc(50,) lc(90) and lc(99) values were recorded for various instar larvae where i instar larvae were found to be the most susce ... | 2010 | 21188602 |
| the role of the aedes aegypti epsilon glutathione transferases in conferring resistance to ddt and pyrethroid insecticides. | the epsilon glutathione transferase (gst) class in the dengue vector aedes aegypti consists of eight sequentially arranged genes spanning 53,645 bp on super contig 1.291, which maps to chromosome 2. one epsilon gst, gste2, has previously been implicated in conferring resistance to ddt. the amino acid sequence of gste2 in an insecticide susceptible and a ddt resistant strain differs at five residues two of which occur in the putative ddt binding site. characterization of the respective recombinan ... | 2011 | 21195177 |
| travel-related mosquito-transmitted disease questionnaire survey among health professionals in taiwan. | health-care professionals can help travelers by providing accurate pre-travel counseling for mosquito-transmitted diseases such as malaria, yellow fever, and dengue fever. governments and international organizations will benefit from knowledge survey among health professionals in this field to promote the development of travel health profession. this study investigates physicians' and nurses' knowledge regarding malaria, yellow fever, and dengue fever. | 2015 | 21199140 |
| the extinction of dengue through natural vulnerability of its vectors. | dengue is the world's most important mosquito-borne viral illness. successful future management of this disease requires an understanding of the population dynamics of the vector, especially in the context of changing climates. our capacity to predict future dynamics is reflected in our ability to explain the significant historical changes in the distribution and abundance of the disease and its vector. | 2010 | 21200424 |
| spatial mapping of gene expression in the salivary glands of the dengue vector mosquito, aedes aegypti. | aedes aegypti mosquitoes are the main vectors of dengue viruses to humans. understanding their biology and interactions with the pathogen are prerequisites for development of dengue transmission control strategies. mosquito salivary glands are organs involved directly in pathogen transmission to vertebrate hosts. information on the spatial distribution of gene expression in these organs is expected to assist in the development of novel disease control strategies, including those that entail the ... | 2011 | 21205315 |
| field efficacy of new larvicide products for control of multi-resistant aedes aegypti populations in martinique (french west indies). | world-wide dengue vector control is hampered by the spread of insecticide resistance in aedes aegypti. we report the resistance status of a wild ae. aegypti population from martinique (vauclin) to conventional larvicides (bacillus thuringiensis var israeliensis [bti] and temephos) and potential alternatives (spinosad, diflubenzuron, and pyriproxyfen). the efficacy and residual activity of these insecticides were evaluated under simulated and field conditions. the vauclin strain exhibited a high ... | 2011 | 21212213 |
| quantitative genetics of aedes aegypti vector competence for dengue viruses: towards a new paradigm? | similar to many other host-pathogen interactions, the vector competence of aedes aegypti for dengue viruses appears to be determined by genotype-by-genotype interactions, whereby the outcome of infection depends on the specific combination of mosquito and virus genotypes. this can complicate efforts to dissect the genetic basis of vector competence in nature because it obscures mapping between genotype and phenotype and brings into question the notion of universal mosquito resistance or suscepti ... | 2011 | 21215699 |
| assessing key safety concerns of a wolbachia-based strategy to control dengue transmission by aedes mosquitoes. | mosquito-borne diseases such as dengue fever, chikungunya or malaria affect millions of people each year and control solutions are urgently needed. an international research program is currently being developed that relies on the introduction of the bacterial endosymbiont wolbachia pipientis into aedes aegypti to control dengue transmission. in order to prepare for open-field testing releases of wolbachia-infected mosquitoes, an intensive social research and community engagement program was unde ... | 2010 | 21225190 |
| insecticide resistance in aedes aegypti populations from ceará, brazil. | organophosphates and pyrethroids are used widely in brazil to control aedes aegypti, the main vector of dengue viruses, under the auspices of the national programme for dengue control. resistance to these insecticides is widespread throughout brazil. in ceará the vector is present in 98% of districts and resistance to temephos has been reported previously. here we measure resistance to temephos and the pyrethroid cypermethrin in three populations from ceará and use biochemical and molecular assa ... | 2011 | 21226942 |
| worldwide patterns of genetic differentiation imply multiple 'domestications' of aedes aegypti, a major vector of human diseases. | understanding the processes by which species colonize and adapt to human habitats is particularly important in the case of disease-vectoring arthropods. the mosquito species aedes aegypti, a major vector of dengue and yellow fever viruses, probably originated as a wild, zoophilic species in sub-saharan africa, where some populations still breed in tree holes in forested habitats. many populations of the species, however, have evolved to thrive in human habitats and to bite humans. this includes ... | 2011 | 21227970 |
| the aquaporin gene family of the yellow fever mosquito, aedes aegypti. | the mosquito, aedes aegypti, is the principal vector of the dengue and yellow fever viruses. during feeding, an adult female can take up more than its own body weight in vertebrate blood. after a blood meal females excrete large amounts of urine through their excretion system, the malpighian tubules (mt). diuresis starts within seconds after the mosquito starts feeding. aquaporins (aqps) are a family of membrane transporters that regulate the flow of water, glycerol and other small molecules acr ... | 2010 | 21249121 |
| induction of a peptide with activity against a broad spectrum of pathogens in the aedes aegypti salivary gland, following infection with dengue virus. | the ultimate stage of the transmission of dengue virus (denv) to man is strongly dependent on crosstalk between the virus and the immune system of its vector aedes aegypti (ae. aegypti). infection of the mosquito's salivary glands by denv is the final step prior to viral transmission. therefore, in the present study, we have determined the modulatory effects of denv infection on the immune response in this organ by carrying out a functional genomic analysis of uninfected salivary glands and sali ... | 2011 | 21249175 |
| distribution of aedes albopictus (diptera: culicidae) in northwestern china. | the asian tiger mosquito, aedes albopictus (diptera: culicidae), has a wide distribution in china with its northwestern limits among seven provinces. during 2006-2008, distribution information was collected in 33 urban and rural areas in those boundary provinces by collecting larvae or adult mosquito from different breeding sites. additional information of seven sites was gathered from local health authorities. three generally accepted climatic delineations affecting distribution of the species ... | 2011 | 21254912 |
| isolation, primary culture and morphological characterization of oenocytes from aedes aegypti pupae. | oenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. in light of the lack of information on oenocytes from aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain ae. aegypti pupa oenocytes in primary culture. ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells wi ... | 2011 | 21255811 |
| the combination of the entomopathogenic fungus metarhizium anisopliae with the insecticide imidacloprid increases virulence against the dengue vector aedes aegypti (diptera: culicidae). | dengue fever transmitted by the mosquito aedes aegypti, is one of the most rapidly spreading insect borne diseases, stimulating the search for alternatives to current control measures. the dengue vector a. aegypti has received less attention than anophelene species, although more than 2.5 billion people are at risk of infection worldwide. entomopathogenic fungi are emerging as potential candidates for the control of mosquitoes. here we continue our studies on the pathogenicity of the entomopatho ... | 2011 | 21266078 |
| ecological modeling of aedes aegypti (l.) pupal production in rural kamphaeng phet, thailand. | aedes aegypti (l.) is the primary vector of dengue, the most important arboviral infection globally. until an effective vaccine is licensed and rigorously administered, ae. aegypti control remains the principal tool in preventing and curtailing dengue transmission. accurate predictions of vector populations are required to assess control methods and develop effective population reduction strategies. ae. aegypti develops primarily in artificial water holding containers. release recapture studies ... | 2011 | 21267055 |
| characterisation of ddt and pyrethroid resistance in trinidad and tobago populations of aedes aegypti. | insecticide resistance is an important factor in the effectiveness of aedes aegypti control and the related spread of dengue. the objectives of this study were to investigate the status of the organochlorine dichlorodiphenyltrichloroethane (ddt) and pyrethroid (permethrin and deltamethrin) resistance in trinidad and tobago populations of ae. aegypti and the underlying biochemical mechanisms. nine populations of ae. aegypti larvae from trinidad and tobago were assayed to ddt and pys using the cen ... | 2011 | 21272394 |
| rna-seq analyses of blood-induced changes in gene expression in the mosquito vector species, aedes aegypti. | hematophagy is a common trait of insect vectors of disease. extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive and reproductive processes, among others. studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. the mosquito aedes aegypti (diptera, culicidae), a vector of dengue viruses, yellow fever virus (yfv) and chikungunya virus (cv), is the sub ... | 2011 | 21276245 |
| replication of the primary dog kidney-53 dengue 2 virus vaccine candidate in aedes aegypti is modulated by a mutation in the 5' untranslated region and amino acid substitutions in nonstructural proteins 1 and 3. | previous studies have demonstrated reduced replication of the cell culture-adapted dengue-2 virus (denv-2) vaccine candidate, primary dog kidney (pdk)-53, compared with the parental denv-2 strain, 16681, in c6/36 cells. various denv-2 mutants incorporating pdk-53 substitutions singly and in combination into the 16681 genetic backbone were used to identify the genetic basis for impaired replication of the vaccine candidate in vitro in aedes aegypti cell culture (aag2 cells) as well as the reduced ... | 2011 | 21284523 |
| initial assessment of the acceptability of a push-pull aedes aegypti control strategy in iquitos, peru and kanchanaburi, thailand. | as part of a larger research program evaluating chemical threshold levels for a push-pull intervention to reduce man-vector (aedes aegypti) contact, this qualitative study explored local perceptions and strategies associated with mosquito control within dengue-endemic communities in peru and thailand. focus groups were used to provide preliminary information that would identify possible public acceptance issues to the push-pull strategy in each site. nine focus group discussions (total of 102 in ... | 2011 | 21292886 |
| estimating the magnitude and direction of altered arbovirus transmission due to viral phenotype. | vectorial capacity is a measure of the transmission potential of a vector borne pathogen within a susceptible population. vector competence, a component of the vectorial capacity equation, is the ability of an arthropod to transmit an infectious agent following exposure to that agent. comparisons of arbovirus strain-specific vector competence estimates have been used to support observed or hypothesized differences in transmission capability. typically, such comparisons are made at a single time ... | 2011 | 21298018 |
| sirna-mediated gene targeting in aedes aegypti embryos reveals that frazzled regulates vector mosquito cns development. | although mosquito genome projects uncovered orthologues of many known developmental regulatory genes, extremely little is known about the development of vector mosquitoes. here, we investigate the role of the netrin receptor frazzled (fra) during embryonic nerve cord development of two vector mosquito species. fra expression is detected in neurons just prior to and during axonogenesis in the embryonic ventral nerve cord of aedes aegypti (dengue vector) and anopheles gambiae (malaria vector). ana ... | 2011 | 21304954 |
| genomic organization and splicing evolution of the doublesex gene, a drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquito aedes aegypti. | in the model system drosophila melanogaster, doublesex (dsx) is the double-switch gene at the bottom of the somatic sex determination cascade that determines the differentiation of sexually dimorphic traits. homologues of dsx are functionally conserved in various dipteran species, including the malaria vector anopheles gambiae. they show a striking conservation of sex-specific regulation, based on alternative splicing, and of the encoded sex-specific proteins, which are transcriptional regulator ... | 2011 | 21310052 |
| [evaluating an information, education and communication (iec) strategy which was adopted for aedes aegypti control in la dorada, colombia]. | estimating the impact of the information, education and communication strategy on aedes aegypti infestation in homes in la dorada, colombia. | 2010 | 21311826 |
| significant increase in travel-associated dengue fever in germany. | increasing numbers of dengue fever (df) cases reflect the increasing travel mobility together with the expanding geographical distribution of the vector aedes aegypti. compared with earlier surveys in germany, higher incidences occur and correlate well with ongoing outbreaks. therefore, we investigated 767 serum samples from 594 returning travellers with suspected df between 2005 and 2010, which where sent from different hospitals in the drainage area frankfurt/main. established diagnostic assay ... | 2011 | 21311908 |
| cocirculation of two dengue virus serotypes in individual and pooled samples of aedes aegypti and aedes albopictus larvae. | to detect dengue virus, eggs of aedes sp were collected in the city of belo horizonte, brazil, in 2007. | 2011 | 21340419 |