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in vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium.an efficient and reliable method is described for the culture of equine strongyles from the third (l3) to the fourth (l4) larval stage. medium consists of 50% fetal calf serum and 50% nctc with additions of l-glutamine, nahco3, yeast extract, bactopeptone, and dextrose. the gas phase used is of prime importance; it is a mixture of 10% co2, 5% o2, and 85% n2. strongylus vulgaris, strongylus edentatus, strongylus equinus, triodontophorus brevicauda, triodontophorus serratus, triodontophorus tenuic ...19948158465
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