| characterization and functional analysis of calmodulin and calmodulin-like genes in fragaria vesca. | calcium is a universal messenger that is involved in the modulation of diverse developmental and adaptive processes in response to various stimuli. calmodulin (cam) and calmodulin-like (cml) proteins are major calcium sensors in all eukaryotes, and they have been extensively investigated for many years in plants and animals. however, little is known about cams and cmls in woodland strawberry (fragaria vesca). in this study, we performed a genome-wide analysis of the strawberry genome and identif ... | 2016 | 27990153 |
| non-host resistance induced by the xanthomonas effector xopq is widespread within the genus nicotiana and functionally depends on eds1. | most gram-negative plant pathogenic bacteria translocate effector proteins (t3es) directly into plant cells via a conserved type iii secretion system, which is essential for pathogenicity in susceptible plants. in resistant plants, recognition of some t3es is mediated by corresponding resistance (r) genes or r proteins and induces effector triggered immunity (eti) that often results in programmed cell death reactions. the identification of r genes and understanding their evolution/distribution b ... | 2016 | 27965697 |
| agrobacterium tumefaciens-mediated transformation as an efficient tool for insertional mutagenesis of cercospora zeae-maydis. | an efficient agrobacterium tumefaciens-mediated transformation (atmt) approach was developed for the plant pathogenic fungus, cercospora zeae-maydis, which is the causative agent of gray leaf spot in maize. the transformation was evaluated with five parameters to test the efficiencies of transformation. results showed that spore germination time, co-cultivation temperature and time were the significant influencing factors in all parameters. randomly selected transformants were confirmed and the ... | 2017 | 27965011 |
| penicillin v acylases from gram-negative bacteria degrade n-acylhomoserine lactones and attenuate virulence in pseudomonas aeruginosa. | virulence pathways in gram-negative pathogenic bacteria are regulated by quorum sensing mechanisms, through the production and sensing of n-acylhomoserine lactone (ahl) signal molecules. enzymatic degradation of ahls leading to attenuation of virulence (quorum quenching) could pave the way for the development of new antibacterials. penicillin v acylases (pvas) belong to the ntn hydrolase superfamily, together with ahl acylases. pvas are exploited widely in the pharmaceutical industry, but their ... | 2017 | 27933456 |
| type iv effector proteins involved in the medicago-sinorhizobium symbiosis. | in this study, we investigated genetic elements of the type iv secretion system (t4ss) found in sinorhizobium spp. and the role they play in symbiosis. sinorhizobium meliloti and s. medicae each contain a putative t4ss similar to that used by agrobacterium tumefaciens during pathogenesis. the cre reporter assay for translocation system was used to validate potential effector proteins. both s. meliloti and s. medicae contained the effector protein tfea, which was translocated into the host plant. ... | 2017 | 27918247 |
| overexpression of a thaumatin-like protein gene from vitis amurensis improves downy mildew resistance in vitis vinifera grapevine. | downy mildew is a highly destructive disease in grapevine production. a gene encoding pathogenesis-related (pr) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "zuoshan-1," a clonal selection from wild vitis amurensis rupr. the predicted thaumatin-like protein (vatlp) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. the full length of the vatlp gene was transformed into somatic embryogenic calli of v. vinifera 'thompson seedless' via ... | 2016 | 27900595 |
| gene function analysis in the ubiquitous human commensal and pathogen malassezia genus. | the genus malassezia includes 14 species that are found on the skin of humans and animals and are associated with a number of diseases. recent genome sequencing projects have defined the gene content of all 14 species; however, to date, genetic manipulation has not been possible for any species within this genus. here, we develop and then optimize molecular tools for the transformation of malassezia furfur and malassezia sympodialis using agrobacterium tumefaciens delivery of transfer dna (t-dna ... | 2016 | 27899504 |
| rhizobium radiobacter in pulmonary abscess associated with postgripal necrotizing pneumonia. | | 2017 | 27897436 |
| plant-phytopathogen interactions: bacterial responses to environmental and plant stimuli. | plant pathogenic bacteria attack numerous agricultural crops, causing devastating effects on plant productivity and yield. they survive in diverse environments, both in plants, as pathogens, and also outside their hosts as saprophytes. hence, they are confronted with numerous changing environmental parameters. during infection, plant pathogens have to deal with stressful conditions, such as acidic, oxidative and osmotic stresses; anaerobiosis; plant defenses; and contact with antimicrobial compo ... | 2016 | 27878915 |
| metabolic response of agrobacterium tumefaciens 5a to arsenite. | wide-spread abundance in soil and water, coupled with high toxicity have put arsenic at the top of the list of environmental contaminants. early studies demonstrated that both concentration and the valence state of inorganic arsenic (arsenite, as(iii) vs. arsenate as(v)) can be modulated by microbes. using genetics, transcriptomic and proteomic techniques, microbe-arsenic detoxification, respiratory as(v) reduction and as(iii) oxidation have since been examined. the effect of arsenic exposure on ... | 2017 | 27871140 |
| arabidopsis nahg plants as a suitable and efficient system for transient expression using agrobacterium tumefaciens. | | 2017 | 27867105 |
| transformation of the cucurbit powdery mildew pathogen podosphaera xanthii by agrobacterium tumefaciens. | the obligate biotrophic fungal pathogen podosphaera xanthii is the main causal agent of powdery mildew in cucurbit crops all over the world. a major limitation of molecular studies of powdery mildew fungi (erysiphales) is their genetic intractability. in this work, we describe a robust method based on the promiscuous transformation ability of agrobacterium tumefaciens for reliable transformation of p. xanthii. the a. tumefaciens-mediated transformation (atmt) system yielded transformants of p. x ... | 2017 | 27864969 |
| expression in grasses of multiple transgenes for degradation of munitions compounds on live-fire training ranges. | the deposition of toxic munitions compounds, such as hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (rdx), on soils around targets in live-fire training ranges is an important source of groundwater contamination. plants take up rdx but do not significantly degrade it. reported here is the transformation of two perennial grass species, switchgrass (panicum virgatum) and creeping bentgrass (agrostis stolonifera), with the genes for degradation of rdx. these species possess a number of agronomic trait ... | 2016 | 27862819 |
| sacb-sacr gene cassette as the negative selection marker to suppress agrobacterium overgrowth in agrobacterium-mediated plant transformation. | agrobacterium overgrowth is a common problem in agrobacterium-mediated plant transformation. to suppress the agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. the antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. the sacb-sacr proteins are toxic to most agrobacterium tumefaciens strains when they are grown on culture medium supplemented with sucrose. therefore, sacb-sacr genes ca ... | 2016 | 27833912 |
| development of marker-free insect-resistant indica rice by agrobacterium tumefaciens-mediated co-transformation. | agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. in this study, the vectors pmf-2a(∗) containing a synthetic cry2a(∗) gene driven by maize ubiquitin promoter and pcambia1301 harboring hygromycin phosphotransferase gene (hpt) were introduced into minghui86 (oryza sativa l. ssp. indica), an elite indica restorer line. two independent transformants containing both the cry2a(∗) gene and hpt gene were regenerated. several homozygous marker-f ... | 2016 | 27833629 |
| t-dna integration in plants results from polymerase-θ-mediated dna repair. | agrobacterium tumefaciens is a pathogenic bacterium, which transforms plants by transferring a discrete segment of its dna, the t-dna, to plant cells. the t-dna then integrates into the plant genome. t-dna biotechnology is widely exploited in the genetic engineering of model plants and crops. however, the molecular mechanism underlying t-dna integration remains unknown(1). here we demonstrate that in arabidopsis thaliana t-dna integration critically depends on polymerase theta (pol θ). we find t ... | 2016 | 27797358 |
| the construction and use of versatile binary vectors carrying pyrg auxotrophic marker and fluorescent reporter genes for agrobacterium-mediated transformation of aspergillus oryzae. | aspergillus oryzae is a safe mold widely used in food industry. it is also considered as a microbial cell factory for production of recombinant proteins and enzymes. currently, genetic manipulation of filamentous fungi is achieved via agrobacterium tumefaciens-mediated transformation methods usually employing antibiotic resistance markers. these methods are hardly usable for a. oryzae due to its strong resistance to the common antifungal compounds used for fungal transformation. in this study, w ... | 2016 | 27804102 |
| fitness costs restrict niche expansion by generalist niche-constructing pathogens. | we investigated the molecular and ecological mechanisms involved in niche expansion, or generalism, versus specialization in sympatric plant pathogens. nopaline-type and octopine-type agrobacterium tumefaciens engineer distinct niches in their plant hosts that provide different nutrients: nopaline or octopine, respectively. previous studies revealed that nopaline-type pathogens may expand their niche to also assimilate octopine in the presence of nopaline, but consequences of this phenomenon on ... | 2017 | 27801902 |
| deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus. | aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ... | 2016 | 27801871 |
| screening auxin response, in vitro culture aptitude and susceptibility to agrobacterium-mediated transformation of italian commercial durum wheat varieties. | the development of a robust agrobacterium-mediated transformation protocol for a recalcitrant species like durum wheat requires the identification and optimization of factors affecting t-dna delivery and plant regeneration. the purpose of this research was to compare the behavior of diverse durum wheat genotypes during in vitro culture and agrobacterium tumefaciens-mediated transformation, using immature embryos as explants. apart from plant genotype, two of the main influencing factors for a su ... | 2016 | 27801844 |
| generation of β-glucuronidase reporter-tagged strain to monitor ustilaginoidea virens infection in rice. | an agrobacterium-mediated genetic transformation system for the rice false smut fungus ustilaginoidea virens was developed using conidia as recipients. a binary vector, pcambia1301-pgpda-gus-ttrpc, was constructed. the gpda promoter (pgpda) from aspergillus nidulans was used to drive the expression of the β-glucuronidase (gus) gene which enabled gus activity visualization. the conidia transformation efficiency reached approximately 110 to 250 transformants per 1×10(5) conidia. based on the analy ... | 2016 | 27789314 |
| isolation of conditional mutations in genes essential for viability of cryptococcus neoformans. | discovering the genes underlying fundamental processes that enable cells to live and reproduce is a technical challenge, because loss of gene function in mutants results in organisms that cannot survive. this study describes a forward genetics method to identify essential genes in fungi, based on the propensity for agrobacterium tumefaciens to insert t-dna molecules into the promoters or 5' untranslated regions of genes and by placing a conditional promoter within the t-dna. insertions of the pr ... | 2016 | 27783209 |
| infiltration-rnaseq: transcriptome profiling of agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants. | transcription factors (tfs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. the identification of genes and transcriptional networks regulated by a tf often requires stable transformation and expression changes in plant cells. however, the production of stable transformants can be slow and laborious with no guarantee of success. furthermore, transgenic plants overexpressing a tf of interest can present pleiotropic phenotypes and/or result in a high numb ... | 2016 | 27777610 |
| characterization of vvpal-like promoter from grapevine using transgenic tobacco plants. | a 2000-bp 5'-flanking region of vvpal-like was isolated from 'summer black' grapevine by pcr amplification, named pvvpal-like. to gain a better understanding of the expression and regulatory mechanism of vvpal-like, a chimeric expression unit consisting of the β-glucuronidase (gus) reporter gene under the control of a 2000-bp fragment of the vvpal-like promoter was transformed into tobacco via agrobacterium tumefaciens. histochemical staining showed that the full-length promoter directs efficien ... | 2016 | 27562678 |
| biocontrol activity and patulin-removal effects of bacillus subtilis, rhodobacter sphaeroides and agrobacterium tumefaciens against penicillium expansum. | this study was conducted to evaluate the biocontrol potential of bacillus subtilis cicc 10034, rhodobacter sphaeroides cgmcc 1.2182 and agrobacterium tumefaciens cgmcc 1.2554 against patulin (pat)-producer penicillium expansum and their ability to remove pat. | 2016 | 27328641 |
| improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (medicago sativa l.) somatic embryos using cuscuta campestris extract. | developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. one of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. for investigating the influence of cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to ... | 2016 | 27729718 |
| overexpressing arabidopsis abf3 increases tolerance to multiple abiotic stresses and reduces leaf size in alfalfa. | arabidopsis abscisic acid-responsive element-binding factor 3 (abf3), a bzip transcription factor, plays an important role in regulating multiple stress responses in plants. overexpressing atabf3 increases tolerance to various stresses in several plant species. alfalfa (medicago sativa l.), one of the most important perennial forage crops worldwide, has high yields, high nutritional value, and good palatability and is widely distributed in irrigated and semi-arid regions throughout the world. ho ... | 2016 | 27721135 |
| diverse profiles of n-acyl-homoserine lactones in biofilm forming strains of cronobacter sakazakii. | the present study investigates the role of quorum sensing (qs) molecules expressed by c. sakazakii in biofilm formation and extracellular polysaccharide expression. the qs signaling was detected using chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) based bioassay. long chain n-acyl-homoserine lactones (ahls) with c6- c18 chain length were identified using high performance liquid chromatography and liquid chromatography-high resolution mass spectrometry. a higher specific ... | 2016 | 27715454 |
| the nonspecific lipid transfer protein atltpi-4 is involved in suberin formation of arabidopsis thaliana crown galls. | nonspecific lipid transfer proteins reversibly bind different types of lipid molecules in a hydrophobic cavity. they facilitate phospholipid transfer between membranes in vitro, play a role in cuticle and possibly in suberin formation, and might be involved in plant pathogen defense signaling. this study focuses on the role of the lipid transfer protein atltpi-4 in crown gall development. arabidopsis (arabidopsis thaliana) crown gall tumors, which develop upon infection with the virulent agrobac ... | 2016 | 27688623 |
| identification of genes associated with asexual reproduction in phyllosticta citricarpa mutants obtained through agrobacterium tumefaciens transformation. | phyllosticta citricarpa is the epidemiological agent of citrus black spot (cbs) disease, which is responsible for large economic losses worldwide. cbs is characterized by the presence of spores (pycnidiospores) in dark lesions of fruit, which are also responsible for short distance dispersal of the disease. the identification of genes involved in asexual reproduction of p. citricarpa can be an alternative for directional disease control. we analyzed a library of mutants obtained through agrobact ... | 2016 | 27664732 |
| recombination-based generation of the agroinfectious clones of peanut stunt virus. | full-length cdna clones of peanut stunt virus strain p (psv-p) were constructed and introduced into nicotiana benthamiana plants via agrobacterium tumefaciens. the cdna fragments corresponding to three psv genomic rnas and satellite rna were cloned into pgreen binary vector between cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator employing seamless recombinational cloning system. the plasmids were delivered into a. tumefaciens, followed by infiltration of hosts ... | 2016 | 27659243 |
| structural analysis and inhibition of trae from the pkm101 type iv secretion system. | gram-negative bacteria use type iv secretion systems (t4sss) for a variety of macromolecular transport processes that include the exchange of genetic material. the pkm101 plasmid encodes a t4ss similar to the well-studied model systems from agrobacterium tumefaciens and brucella suis here, we studied the structure and function of trae, a homolog of virb8 that is an essential component of all t4sss. analysis by x-ray crystallography revealed a structure that is similar to other virb8 homologs but ... | 2016 | 27634044 |
| construction and quality analysis of transgenic rehmannia glutinosa containing tmv and cmv coat protein. | plant viruses, especially tobacco mosaic virus (tmv) and cucumber mosaic virus (cmv) are serious threats to rehmannia glutinosa which is a "top grade" herb in china. in the present study, tmv- and cmv-resistant rehmannia glutinosa libosch. plants were constructed by transforming the protein (cp) genes of tmv and cmv into rehmannia glutinosa via a modified procedure of agrobacterium tumefaciens-mediated transformation. integration and expression of tmv cp and cmv cp transgenes in 2 lines, lba-1 a ... | 2016 | 27618888 |
| function analysis of caffeoyl-coa o-methyltransferase for biosynthesis of lignin and phenolic acid in salvia miltiorrhiza. | in this study, we cloned a full-length cdna and the genomic dna sequence of smccoaomt (genbank id jq007585) from salvia miltiorrhiza. the 744-bp open-reading frame encodes a protein of 247 amino acids that shares 95 % similarity with one in vitis vinifera. real-time quantitative pcr analysis revealed that smccoaomt is most highly expressed in the stems and can be induced by methyl jasmonate (meja) and xc-1 treatment. to evaluate its function in vivo, we generated rna interference transgenic plan ... | 2017 | 27613617 |
| structural basis for high specificity of amadori compound and mannopine opine binding in bacterial pathogens. | agrobacterium tumefaciens pathogens genetically modify their host plants to drive the synthesis of opines in plant tumors. opines are either sugar phosphodiesters or the products of condensed amino acids with ketoacids or sugars. they are agrobacterium nutrients and imported into the bacterial cell via periplasmic-binding proteins (pbps) and abc-transporters. mannopine, an opine from the mannityl-opine family, is synthesized from an intermediate named deoxy-fructosyl-glutamine (dfg), which is al ... | 2016 | 27609514 |
| identification of conidiogenesis-associated genes in colletotrichum gloeosporioides by agrobacterium tumefaciens-mediated transformation. | the colletotrichum gloeosporioides is one of the most significant pathogens leading to huge economic losses. to infect plants and cause disease dissemination, the fungus elaborates to produce asexual spores called conidia, which are long-lived and highly resistant to environmental stresses. here, we report a large-scale, systematic genome-wide screening of conidiogenesis-associated genes via conidiation assays, and high-efficiency tail-pcrs. of 10,210 independent transformants tested, 59 mutants ... | 2016 | 27582094 |
| dna labelling of varieties covered by patent protection: a new solution for managing intellectual property rights in the seed industry. | plant breeders' rights are undergoing dramatic changes due to changes in patent rights in terms of plant variety rights protection. although differences in the interpretation of »breeder's exemption«, termed research exemption in the 1991 upov, did exist in the past in some countries, allowing breeders to use protected varieties as parents in the creation of new varieties of plants, current developments brought about by patenting conventionally bred varieties with the european patent office (suc ... | 2017 | 27567633 |
| biological responses of symbiotic rhizobium radiobacter strain vbck1062 to the arsenic contaminated rhizosphere soils of mung bean. | the rationale could be that mung bean is cultivated in areas of arsenic contamination and therefore it is worth investigating how rhizobium is impacted by arsenic exposure. the objective(s) of the study deals with relationship between rhizobium metal tolerance and its adaptations to metal stressed environment. the selected strain was recovered from root nodules of vigna radiata, based on viscous eps production and arsenic tolerant capacity, identified as r. radiobacter by 16s rdna sequencing. ba ... | 2016 | 27566287 |
| insertion mutation in hmg-coa lyase increases the production yield of mpa through agrobacterium tumefaciens-mediated transformation. | mycophenolic acid (mpa) is an antibiotic produced by penicillium brevicompactum. mpa has antifungal, antineoplastic, and immunosuppressive functions, among others. β-hydroxy-β-methylglutaryl-coa (hmg-coa) lyase is a key enzyme in the bypass metabolic pathway. the inhibitory activity of hmg-coa lyase increases the mpa biosynthetic flux by reducing the generation of by-products. in this study, we cloned the p. brevicompactum hmg-coa lyase gene using the thermal asymmetric interlaced polymerase cha ... | 2016 | 27558440 |
| production of engineered minichromosome vectors via the introduction of telomere sequences. | artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. the production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing transgenes and telomere sequences concurrently to the cell to truncate an endogenous chromosomal target. two methods can be utilized; either the telomere sequences can be incorporated into a binary vector for transformation with agrobacterium tumefaciens, ... | 2016 | 27557682 |
| the use of t-dna insertional mutagenesis to improve cellulase production by the thermophilic fungus humicola insolens y1. | humicola insolens is an excellent producer of ph-neutral active, thermostable cellulases that find many industrial applications. in the present study, we developed an efficient agrobacterium tumefaciens-mediated transformation system for h. insolens. we transformed plasmids carrying the promoter of the glyceraldehyde-3-phosphate dehydrogenase gene of h. insolens driving the transcription of genes encoding neomycin phosphotransferase, hygromycin b phosphotransferase, and enhanced green fluorescen ... | 2016 | 27506519 |
| characterization of t-dna insertion mutants with decreased virulence in the entomopathogenic fungus beauveria bassiana jef-007. | the bean bug, riptortus pedestris, is a major agricultural pest that reduces crop quality and value. chemical pesticides have contributed to pest management, but resistance to these chemicals has significantly limited their use. alternative strategies with different modes of action, such as entomopathogenic fungi, are therefore of great interest. herein, we explored how entomopathogenic fungi can potentially be used to control the bean bug and focused on identifying virulence-related genes. beau ... | 2016 | 27470140 |
| a potent bidirectional promoter from the monocot cereal eleusine coracana. | ragi bifunctional α-amylase-trypsin inhibitor (rbi) of eleusine coracana (l.) gaertn. (finger millet) simultaneously inhibits α-amylase and trypsin. in continuation of previous work on the cloning, expression and characterization of rbi, a bidirectional promoter from finger millet was explored on the basis of experimental observations. two trypsin inhibitors were identified while purifying rbi from a trypsin-sepharose column eluent. using an fplc gel filtration column, these three inhibitors wer ... | 2016 | 27460530 |
| inhibition of quorum-sensing-mediated biofilm formation in cronobacter sakazakii strains. | the present study investigated plant extracts for their anti-quorum-sensing (qs) potential to inhibit the biofilm formation in cronobacter sakazakii strains. the bioassay based on loss of pigment production by chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) was used for initial screening of the extracts. further, the effect of extracts on the inhibition of qs-mediated biofilm in c. sakazakii isolates was evaluated using standard crystal violet assay. the effect on biofilm ... | 2016 | 27460095 |
| protoplast transformation as a potential platform for exploring gene function in verticillium dahliae. | large efforts have focused on screening for genes involved in the virulence and pathogenicity of verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the plant is infected. although agrobacterium tumefaciens-mediated transformation (atmt) has been widely used for gene screening, a quick and easy method has been needed to facilitate transformation. | 2016 | 27455996 |
| bioactive dibenzo-α-pyrone derivatives from the endophytic fungus rhizopycnis vagum nitaf22. | six new dibenzo-α-pyrones, rhizopycnolides a (1) and b (2) and rhizopycnins a-d (3-6), together with eight known congeners (7-14), were isolated from the endophytic fungus rhizopycnis vagum nitaf22 obtained from nicotiana tabacum. the structures of the new compounds were unambiguously elucidated using nmr, hresims, tddft ecd calculation, and x-ray crystallography data. rhizopycnolides a (1) and b (2) feature an uncommon γ-butyrolactone-fused dibenzo-α-pyrone tetracyclic skeleton (6/6/6/5), while ... | 2016 | 27441892 |
| testing transgenic aspen plants with bar gene for herbicide resistance under semi-natural conditions. | obtaining herbicide resistant plants is an important task in the genetic engineering of forest trees. transgenic european aspen plants (populus tremula l.) expressing the bar gene for phosphinothricin resistance have been produced using agrobacterium tumefaciens-mediated transformation. successful genetic transformation was confirmed by pcr analysis for thirteen lines derived from two elite genotypes. in 2014-2015, six lines were evaluated for resistance to herbicide treatment under semi-natural ... | 2017 | 27437143 |
| in vitro regeneration and optimization of factors affecting agrobacterium mediated transformation in artemisia pallens, an important medicinal plant. | artemisia pallens is an important medicinal plant. in-vitro regeneration and multiplication of a. pallens have been established using attached cotyledons. different growth regulators were considered for regeneration of multiple shoots. an average of 36 shoots per explants were obtained by culturing attached cotyledons on murashige and skoog's medium containing 2 mg/l bap and 0.1 mg/l naa, after 45 days. the shoots were rooted best on half murashige and skoog's medium with respect to media contai ... | 2016 | 27436917 |
| chimeric coupling proteins mediate transfer of heterologous type iv effectors through the escherichia coli pkm101-encoded conjugation machine. | bacterial type iv secretion systems (t4sss) are composed of two major subfamilies, conjugation machines dedicated to dna transfer and effector translocators for protein transfer. we show here that the escherichia coli pkm101-encoded conjugation system, coupled with chimeric substrate receptors, can be repurposed for transfer of heterologous effector proteins. the chimeric receptors were composed of the n-terminal transmembrane domain of pkm101-encoded traj fused to soluble domains of vird4 homol ... | 2016 | 27432829 |
| transient expression in nicotiana benthamiana for rapid functional analysis of genes involved in non-photochemical quenching and carotenoid biosynthesis. | plants must switch rapidly between light harvesting and photoprotection in response to environmental fluctuations in light intensity. this switch can lead to losses in absorbed energy usage, as photoprotective energy dissipation mechanisms can take minutes to hours to fully relax. one possible way to improve photosynthesis is to engineer these energy dissipation mechanisms (measured as non-photochemical quenching of chlorophyll a fluorescence, npq) to induce and relax more quickly, resulting in ... | 2016 | 27407008 |
| spermidine inversely influences surface interactions and planktonic growth in agrobacterium tumefaciens. | in bacteria, the functions of polyamines, small linear polycations, are poorly defined, but these metabolites can influence biofilm formation in several systems. transposon insertions in an ornithine decarboxylase (odc) gene in agrobacterium tumefaciens, predicted to direct synthesis of the polyamine putrescine from ornithine, resulted in elevated cellulose. null mutants for odc grew somewhat slowly in a polyamine-free medium but exhibited increased biofilm formation that was dependent on cellul ... | 2016 | 27402627 |
| a method for amplification of unknown flanking sequences based on touchdown pcr and suppression-pcr. | thermal asymmetric staggered pcr is the most widely used technique to obtain the flanking sequences. however, it has some limitations, including a low rate of positivity, and complex operation. in this study, a improved method of it was made based on suppression-pcr and touchdown pcr. the pcr fragment obtained by the amplification was used directly for sequencing after gel purification. using this improved method, the positive rate of amplified flanking sequences of the atmt mutants reached 99%. ... | 2016 | 27393656 |
| cloning-free genome engineering in sinorhizobium meliloti advances applications of cre/loxp site-specific recombination. | the soil-dwelling α-proteobacterium sinorhizobium meliloti serves as model for studies of symbiotic nitrogen fixation, a highly important process in sustainable agriculture. here, we report advancements of the genetic toolbox accelerating genome editing in s. meliloti. the hsdmsr operon encodes a type-i restriction-modification (r-m) system. transformation of s. meliloti is counteracted by the restriction endonuclease hsdr degrading dna which lacks the appropriate methylation pattern. we provide ... | 2016 | 27393468 |
| application of a delta-6 desaturase with α-linolenic acid preference on eicosapentaenoic acid production in mortierella alpina. | delta-6 desaturase (fads6) is a key bifunctional enzyme desaturating linoleic acid (la) or α-linolenic acid (ala) in the biosynthesis of polyunsaturated fatty acids (pufas). in previous work, we analyzed the substrate specificity of two fads6 enzymes from mortierella alpina atcc 32222 (mafads6) and micromonas pusilla ccmp1545 (mpfads6), which showed preference for la and ala, respectively. we also clarified the pufa profiles in m. alpina, where these lipids were synthesized mainly via the ω6 pat ... | 2016 | 27364006 |
| rapid and efficient agrobacterium-mediated transformation of sorghum (sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker. | a rapid and efficient agrobacterium -mediated transformation system in sorghum has been developed employing standard binary vectors and bar gene as a selectable marker. sorghum (sorghum bicolor) is an important food and biofuel crop worldwide, for which improvements in genetic transformation are needed to study its biology and facilitate agronomic and commercial improvement. here, we report optimization of regeneration and transformation of public sorghum genotype p898012 using standard binary v ... | 2016 | 27350252 |
| characterization, correction and de novo assembly of an oxford nanopore genomic dataset from agrobacterium tumefaciens. | the minion is a portable single-molecule dna sequencing instrument that was released by oxford nanopore technologies in 2014, producing long sequencing reads by measuring changes in ionic flow when single-stranded dna molecules translocate through the pores. while minion long reads have an error rate substantially higher than the ones produced by short-read sequencing technologies, they can generate de novo assemblies of microbial genomes, after an initial correction step that includes alignment ... | 2016 | 27350167 |
| a cytometry microparticle platform approach for screening tobacco microrna changes after agrobacterium delivery. | micrornas are a class of non-coding regulatory rnas that can modulate development as well as alter innate antiviral defenses in plants. in this study we explored changes in nicotiana benthamiana tobacco microrna expression as it relates to expression of a recombinant anti-ebola gp1 antibody. the antibody was delivered to tobacco leaves through a bacterial agrobacterium tumefaciens "agroinfiltration" expression strategy. a multiplex microparticle-based cytometry assay tracked the expression chang ... | 2016 | 27343681 |
| adjusting microbiome profiles for differences in microbial load by spike-in bacteria. | next-generation 16s ribosomal rna gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. however, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. | 2016 | 27329048 |
| agrobacterium-mediated transformation of fusarium proliferatum. | fusarium proliferatum is an important pathogen that is associated with plant diseases and primarily affects aerial plant parts by producing different mycotoxins, which are toxic to humans and animals. within the last decade, this fungus has also been described as one of the causes of red root rot or sudden death syndrome in soybean, which causes extensive damage to this crop. this study describes the agrobacterium tumefaciens-mediated transformation of f. proliferatum as a tool for the disruptio ... | 2016 | 27323127 |
| enhanced curdlan production with nitrogen feeding during polysaccharide synthesis by rhizobium radiobacter. | curdlan is a secondary metabolite synthesized by agrobacterium sp. and some other bacteria. a newly isolated exopolysaccharide-producing strain was identified to be rhizobium radiobacter cgmcc 12099. the polysaccharide product was confirmed to be curdlan with a molecule weight of 1.4×10(5)da, and its molecular structure was determined by hplc and infrared spectrum. although nitrogen source is necessary for cell reproduction, curdlan production is largely dependent on nitrogen limitation, as well ... | 2016 | 27312649 |
| the rickettsia type iv secretion system: unrealized complexity mired by gene family expansion. | many prokaryotes utilize type iv secretion systems (t4sss) to translocate substrates (e.g. nucleoprotein, dna, protein) across the cell envelope, and/or to elaborate surface structures (i.e. pili or adhesins). among eight distinct t4ss classes, p-t4sss are typified by the agrobacterium tumefaciens vir t4ss, which is comprised of 12 scaffold components (virb1-virb11, vird4). while most p-t4sss include all 12 vir proteins, some differ from the vir archetype by either containing additional scaffold ... | 2016 | 27307105 |
| low-affinity iron transport protein uvt3277 is important for pathogenesis in the rice false smut fungus ustilaginoidea virens. | ustilaginoidea virens is the causal agent of rice false smut disease resulting in quantitative and qualitative losses in rice. to gain insights into the pathogenic mechanisms of u. virens, we established a t-dna insertion mutant library of u. virens through agrobacterium tumefaciens-mediated transformation and selected an enhanced pathogenicity mutant (i.e., b3277). we analyzed the biological characteristics of the wild-type p1 and b3277. the growth rate and sporulation of b3277 were decreased c ... | 2017 | 27306226 |
| the novel gene vppr4-1 from vitis pseudoreticulata increases powdery mildew resistance in transgenic vitis vinifera l. | pathogenesis-related proteins (prs) can lead to increased resistance of the whole plant to pathogen attack. here, we isolate and characterize a pr-4 protein (vppr4-1) from a wild chinese grape vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. its expression profiles under a number of abiotic stresses were also investigated. powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the vppr4-1 induction while nacl an ... | 2016 | 27303413 |
| thermodynamically optimal whole-genome tiling microarray design and validation. | microarray is an efficient apparatus to interrogate the whole transcriptome of species. microarray can be designed according to annotated gene sets, but the resulted microarrays cannot be used to identify novel transcripts and this design method is not applicable to unannotated species. alternatively, a whole-genome tiling microarray can be designed using only genomic sequences without gene annotations, and it can be used to detect novel rna transcripts as well as known genes. the difficulty wit ... | 2016 | 27295952 |
| the pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in arabidopsis thaliana plants. | here, we present the application of microbiology and biotechnology for the production of recombinant pharmaceutical proteins in plant cells. to the best of our knowledge and belief it is one of few examples of the expression of the prokaryotic staphylokinase (sak) in the eukaryotic system. despite the tremendous progress made in the plant biotechnology, most of the heterologous proteins still accumulate to low concentrations in plant tissues. therefore, the composition of expression cassettes to ... | 2016 | 27263008 |
| agrobacterium tumefaciens-mediated transformation of botryosphaeria dothidea. | botryosphaeria dothidea is a severe causal agent of die-back and cankers of many woody plants and causes great losses in many regions. the pathogenic mechanism of this pathogen has not been well explored due to lack of mutants and genetic information. in this study, we developed an agrobacterium tumefaciens-mediated transformation (atmt) protocol for b. dothidea protoplasts using vector pbht2 containing the hph gene as a selection marker under the control of trp c promoter. using this protocol w ... | 2016 | 27263001 |
| lipopeptides from a novel bacillus methylotrophicus 39b strain suppress agrobacterium crown gall tumours on tomato plants. | this study aims to characterise the antibacterial activity of a novel bacillus methylotrophicus strain named 39b against tumourigenic agrobacterium tumefaciens c58 and b6 strains. it also aims to identify the compound that is responsible for its activity and to evaluate its efficiency to control crown gall disease in tomato plants. | 2017 | 27252109 |
| rol genes enhance the biosynthesis of antioxidants in artemisia carvifolia buch. | the secondary metabolites of the artemisia genus are well known for their important therapeutic properties. this genus is one of the valuable sources of flavonoids and other polyphenols, but due to the low contents of these important metabolites, there is a need to either enhance their concentration in the original plant or seek alternative sources for them. the aim of the current study was to detect and enhance the yield of antioxidant compounds of artemisia carvifolia buch. hplc analysis was p ... | 2016 | 27251864 |
| diaporthe endophytica and d. terebinthifolii from medicinal plants for biological control of phyllosticta citricarpa. | the citrus industry is severely affected by citrus black spot (cbs), a disease caused by the pathogen phyllosticta citricarpa. this disease causes loss of production, decrease in the market price of the fruit, and reduction in its export to the european union. currently, cbs disease is being treated in orchards with various pesticides and fungicides every year. one alternative to cbs disease control without harming the environment is the use of microorganisms for biological control. diaporthe en ... | 2017 | 27242153 |
| in vivo rna visualization in plants using ms2 tagging. | intracellular trafficking and asymmetric localization of rna molecules within cells are a prevalent process across phyla involved in developmental control and signaling and thus in the determination of cell fate. in addition to intracellular localization, plants support the trafficking of rna molecules also between cells through plasmodesmata (pd), which has important roles in the cell-to-cell and systemic communication during plant growth and development. viruses have developed strategies to ex ... | 2016 | 27241752 |
| rhizobium radiobacter endocarditis in an intravenous drug user: clinical presentation, diagnosis, and treatment. | rhizobium radiobacter, a soil-based organism, is not, usually, pathogenic unless in the immunecompromised. endocarditis, in the immunocompromised, is a typical presentation generally as a result of catheter-based infections. we describe the presentation of r. radiobacter prosthetic valve endocarditis and the inherent challenges in its presentation and diagnosis. a patient presented with acute limb ischemia secondary to r. radiobacter-mediated endocarditis and subsequent thromboembolization of th ... | 2016 | 27239001 |
| the (unusual) aspartic acid in the metal coordination sphere of the prokaryotic zinc finger domain. | the possibility of choices of protein ligands and coordination geometries leads to diverse zn(ii) binding sites in zinc-proteins, allowing a range of important biological roles. the prokaryotic cys2his2 zinc finger domain (originally found in the ros protein from agrobacterium tumefaciens) tetrahedrally coordinates zinc through two cysteine and two histidine residues and it does not adopt a correct fold in the absence of the metal ion. ros is the first structurally characterized member of a fami ... | 2016 | 27238756 |
| [microbial composition of various surfaces of skin during develop- ment of atopic dermatitis based on data from maldi-tof mass-spectrometry identification method]. | study transformation of skin microflora during development of atopic dermatitis. | 2016 | 27228668 |
| in vitro analysis of essential binding sites on the promoter of the serratia marcescens spn operon with the quorum-sensing receptor spnr. | the n-acylhomoserine lactone (ahl) receptor spnr is a luxr family protein that acts as a negative regulator of ahl-dependent quorum sensing (qs). spnr binds to dna in serratia marcescens as-1 via the spn box; however, the binding affinity of spnr with the nucleotides on the spn box has not yet been investigated. in this study, we used an spn-box-modified sensor electrode, and quartz crystal microbalance analysis demonstrated a drastic reduction of the uptake of spnr. the nucleotides g5 and c16 a ... | 2016 | 27217017 |
| influence of volatile organic compounds emitted by pseudomonas and serratia strains on agrobacterium tumefaciens biofilms. | the ability to form biofilms plays an important role in bacteria-host interactions, including plant pathogenicity. in this work, we investigated the action of volatile organic compounds (vocs) produced by rhizospheric strains of pseudomonas chlororaphis 449, pseudomonas fluorescens b-4117, serratia plymuthica ic1270, as well as serratia proteamaculans strain 94, isolated from spoiled meat, on biofilms formation by three strains of agrobacterium tumefaciens which are causative agents of crown-gal ... | 2016 | 27214244 |
| molybdate uptake by agrobacterium tumefaciens correlates with the cellular molybdenum cofactor status. | many enzymes require the molybdenum cofactor, moco. under mo-limiting conditions, the high-affinity abc transporter modabc permits molybdate uptake and moco biosynthesis in bacteria. under mo-replete conditions, escherichia coli represses modabc transcription by the one-component regulator, mode, consisting of a dna-binding and a molybdate-sensing domain. instead of a full-length mode protein, many bacteria have a shorter mode protein, mode(s) , consisting of a dna-binding domain only. here, we ... | 2016 | 27196733 |
| an improved binary vector and escherichia coli strain for agrobacterium tumefaciens-mediated plant transformation. | the plasmid vector pgreenii is widely used to produce plant transformants via a process that involves propagation in escherichia coli however, we show here that pgreenii-based constructs can be unstable in e. coli as a consequence of them hampering cell division and promoting cell death. in addition, we describe a new version of pgreenii that does not cause these effects, thereby removing the selective pressure for mutation, and a new strain of e. coli that better tolerates existing pgreenii-bas ... | 2016 | 27194805 |
| agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptii gene as a plant selection marker. | transformation of commercially important indica cultivars remains challenging for the scientific community even though agrobacterium-mediated transformation protocols for a few indica rice lines have been well established. we report successful transformation of a commercially important restorer line jk1044r of indica rice hybrid jkrh 401. while following existing protocol, we optimized several parameters for callusing, regeneration and genetic transformation of jk1044r. calli generated from the ... | 2016 | 27186018 |
| engineering of corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars. | xylitol is a non-fermentable sugar alcohol used as sweetener. corynebacterium glutamicum atcc13032 was metabolically engineered for xylitol production from the lignocellulosic pentose sugars xylose and arabinose. direct conversion of xylose to xylitol was achieved through the heterologous expression of nad(p)h-dependent xylose reductase (xr) gene from rhodotorula mucilaginosa. xylitol synthesis from arabinose was attained through polycistronic expression of l-arabinose isomerase (araa), d-psicos ... | 2016 | 27184428 |
| expression of ipt in asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) alters tree architecture, delays leaf senescence, and changes leaf essential oil composition. | the ipt gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. we introduced ipt under the control of the camv35s promoter into asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) via stable agrobacterium tumefaciens-mediated transformation. three of 3-year-old transgenic asakura-sanshoo lines y5, y16, and y17 were used to evaluate the effects of ipt expression on the morphological characteristics, leaf senescence, and essential oil composition. ... | 2017 | 27182107 |
| agrobacterium-mediated transformation of the β-subunit gene in 7s globulin protein in soybean using rnai technology. | the objective of this study was to use rna interference (rnai) to improve protein quality and decrease anti-nutritional effects in soybean. agrobacterium tumefaciens-mediated transformation was conducted using rnai and an expression vector containing the 7s globulin β-subunit gene. the bar gene was used as the selective marker and cotyledonary nodes of soybean genotype jinong 27 were chosen as explant material. regenerated plants were detected by molecular biology techniques. transformation of t ... | 2016 | 27173254 |
| plant gaba:proline ratio modulates dissemination of the virulence ti plasmid within the agrobacterium tumefaciens hosted population. | accumulation of amino acids is a common plant response to several biotic and abiotic stresses, even if the roles of these accumulations remain often poorly understood. in a recent study we measured the levels of different amino acids in tumors of arabidopsis thaliana induced by the phytopathogen agrobacterium tumefaciens and correlated these data with changes of gene expressions in both organisms. this led to the demonstration that the non-protein amino acid gaba plays an important role for the ... | 2016 | 27110651 |
| biotechnological approaches for biomass and cardenolide production in digitalis purpurea l. | digitalis purpurea l. is one of the main economically viable sources of cardenolides (cardiac glycosides) for the pharmaceutical industry. nevertheless, production of cardenolides in plants grown by traditional agriculture is not always an efficient process and can be affected by biotic and abiotic factors. this chapter provides two biotechnology strategies for biomass and cardenolide production in d. purpurea. firstly, we report biomass production using a temporary immersion system (tis), combi ... | 2016 | 27108311 |
| rhizobium radiobacter infection in a 27-year-old african american woman with munchausen syndrome. | rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (hiv) associated immunosuppression, who have catheter or medical device-related febrile neutropenia; treatment involves removal of the catheter or implanted medical device.(1)herein, we report a case of a 27-year-old african american woman with sic ... | 2016 | 27107290 |
| genome sequence of the nicotine-degrading agrobacterium tumefaciens s33. | agrobacterium tumefaciens s33 is capable of growing with nicotine as the sole source of carbon and nitrogen, and has the potential to dispose of tobacco wastes and transform nicotine into functionalized pyridines intermediates, which are important precursors for some valuable drugs and insecticides. here we report the complete genome sequence of strain s33 and predict the gene cluster involved in nicotine catabolism according to the annotation. | 2016 | 27106695 |
| crispr/cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations. | the crispr/cas9 system has successfully been used in various organisms for precise targeted gene editing. although it has been demonstrated that crispr/cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the crispr/cas9 system in tomato plants have not yet been elucidated in detail. in this study, two genes, slpds and slpif4, were used for testing targeted mutagenesis in tomato plants through an agrobacterium tumefaci ... | 2016 | 27097775 |
| uvhog1 is important for hyphal growth and stress responses in the rice false smut fungus ustilaginoidea virens. | rice false smut caused by ustilaginoidea virens is one of the most important diseases of rice worldwide. although its genome has been sequenced, to date there is no report on targeted gene deletion in u. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. in this study, we attempted to generate knockout mutants of the ortholog of yeast hog1 map kinase gene in u. virens. one uvhog1 deletion mutant was identified after screening ov ... | 2016 | 27095476 |
| variation in growth, colonization of maize, and metabolic parameters of gfp- and dsred-labeled fusarium verticillioides strains. | autofluorescent proteins are frequently applied as visual markers in the labeling of filamentous fungi. genes gfp and dsred were transformed into the genome of fusarium verticillioides via the agrobacterium tumefaciens-mediated transformation method. the selected transformants displayed a bright green or red fluorescence in all the organelles of the growing fungal mycelia and spores (except for the vacuoles) both in cultures and in the maize (zea mays) roots they colonized. the results of gene-s ... | 2016 | 27088391 |
| gas chromatography coupled with mass spectrometric characterization of curcuma longa: protection against pathogenic microbes and lipid peroxidation in rat's tissue homogenate. | the present study was designed to investigate the mineral content and antimicrobial activity of curcuma longa extracts and its essential oil. we also determined the lipid peroxidation inhibition activity of the ethanolic extract against sodium nitroprusside (snp) induced thiobarbituric acid reactive species (tbars) formation in rat's brain, kidney and liver homogenates. major constituents of essential oil identified by gas chromatography and mass spectrometry (gcms) were beta-sesquiphellandrene ... | 2016 | 27087084 |
| development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene. | cotton leaf curl virus (clcuv) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. the transgenic cotton (gossypium hirsutum cv. coker 310) plants were developed by using βc1 gene in antisense orientation gene driven by cauliflower mosaic virus-35s promoter and nos (nopaline synthase) terminator and mediated by agrobacterium tumefaciens transformation and somatic embryogenesis system. molecular confirmation of the transformants was carried ... | 2016 | 27081361 |
| a plant-based transient expression system for the rapid production of malaria vaccine candidates. | there are currently no vaccines that provide sterile immunity against malaria. various proteins from different stages of the plasmodium falciparum life cycle have been evaluated as vaccine candidates, but none of them have fulfilled expectations. therefore, combinations of key antigens from different stages of the parasites life cycle may be essential for the development of efficacious malaria vaccines. following the identification of promising antigens using bioinformatics, proteomics, and/or i ... | 2016 | 27076325 |
| transient expression of viral proteins in plants using agrobacterium tumefaciens. | transient expression of viral proteins in plants is a novel alternative to other expression platforms. the viral proteins can be used as potential vaccines or in diagnostics. nicotiana benthamiana leaves or whole plants are infiltrated with recombinant agrobacterium that harbor the gene of interest. protein expression in the plants is rapid and results are obtained within 2-7 days. here we describe how to make electrocompetent agrobacterium, how to transform agrobacterium, how to infiltrate leav ... | 2016 | 27076324 |
| the n-terminal fragment of the tomato torrado virus rna1-encoded polyprotein induces a hypersensitive response (hr)-like reaction in nicotiana benthamiana. | the hypersensitive response (hr) is a defence reaction observed during incompatible plant-pathogen interactions in plants infected with a wide range of fungi, bacteria and viruses. here, we show that an n-terminal polyprotein fragment encoded by tomato torrado virus rna1, located between the first atg codon and the protease cofactor (proco) motif, induces an hr-like reaction in nicotiana benthamiana. agrobacterium tumefaciens-mediated transient expression of the first 105 amino acids (the calcul ... | 2016 | 27072852 |
| molecularly characterized solvent extracts and saponins from polygonum hydropiper l. show high anti-angiogenic, anti-tumor, brine shrimp, and fibroblast nih/3t3 cell line cytotoxicity. | polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. this study was designed to investigate the anti-angiogenic, anti-tumor, and cytotoxic potentials of different solvent extracts and isolated saponins. samples were analyzed using gc, gas chromatography-mass spectrometry (gc-ms) to identify major and bioactive compounds. quantitation of antiangiogenesis for the plant's samples including methanolic extract (ph.cr), its subsequent fractions; n-hexane (ph.hex), chl ... | 2016 | 27065865 |
| artificial agrobacterium tumefaciens strains exhibit diverse mechanisms to repress xanthomonas oryzae pv. oryzae-induced hypersensitive response and non-host resistance in nicotiana benthamiana. | xanthomonas oryzae pv. oryzae (xoo) rapidly triggers a hypersensitive response (hr) and non-host resistance in its non-host plant nicotiana benthamiana. here, we report that agrobacterium tumefaciens strain gv3101 blocks xoo-induced hr in n. benthamiana when pre-infiltrated or co-infiltrated, but not when post-infiltrated at 4 h after xoo inoculation. this suppression by a. tumefaciens is local and highly efficient to xoo. the hr-inhibiting efficiency of a. tumefaciens is strain dependent. strai ... | 2017 | 27061769 |
| development of marker-free transgenic lettuce resistant to mirafiori lettuce big-vein virus. | lettuce big-vein disease caused by mirafiori lettuce big-vein virus (mlbvv) is found in major lettuce production areas worldwide, but highly resistant cultivars have not yet been developed. to produce mlbvv-resistant marker-free transgenic lettuce that would have a transgene with a promoter and terminator of lettuce origin, we constructed a two t-dna binary vector, in which the first t-dna contained the selectable marker gene neomycin phosphotransferase ii, and the second t-dna contained the let ... | 2016 | 27055463 |
| production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture. | pompe disease is a fatal genetic muscle disorder caused by a deficiency of acid α-glucosidase (gaa), a glycogen-degrading lysosomal enzyme. in this study, the human gaa cdna gene was synthesized from human placenta cells and cloned into a plant expression vector under the control of the rice α-amylase 3d (ramy3d) promoter. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin) mediated by agrobacterium tumefaciens. genomic dna pcr and northern blot analysis were ... | 2016 | 27050503 |
| rnai-mediated resistance against cotton leaf curl disease in elite indian cotton (gossypium hirsutum) cultivar narasimha. | cotton leaf curl disease (clcud) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. clcud is a serious threat for the cultivation of cotton (gossypium sp.) and several species in the family malvaceae. in this study, rnai-based approach was applied to generate transgenic cotton (gossypium hirsutum) plants resistant to cotton leaf curl rajasthan virus (clcurv). an intron hairpin (ihp) rnai construct capa ... | 2016 | 27028623 |
| environment control to improve recombinant protein yields in plants based on agrobacterium-mediated transient gene expression. | agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. to achieve economically feasible upstream production and downstream processing, two yield parameters should be considered: (1) recombinant protein content per unit biomass and (2) recombinant protein productivity per unit area-time at the end of the upstream production. because environmental factors in the upstream production have impacts on these parameters, en ... | 2016 | 27014686 |
| agrobacterium-mediated stable genetic transformation of populus angustifolia and populus balsamifera. | the present study demonstrates agrobacterium tumefaciens-mediated stable genetic transformation of two species of poplar - populus angustifolia and populus balsamifera. the binary vector pcambia-npro-long-luc containing the luciferase reporter gene was used to transform stem internode and axillary bud explants. putative transformants were regenerated on selection-free medium using our previously established in vitro regeneration method. explant type, genotype, effect of pre-culture, agrobacteriu ... | 2016 | 27014319 |