| antibiotic treatment with ampicillin accelerates the healing of colonic damage impaired by aspirin and coxib in the experimental colitis. importance of intestinal bacteria, colonic microcirculation and proinflammatory cytokines. | non-steroidal anti-inflammatory drugs (nsaids) are widely used for their anti-inflammatory, analgesic and antipyretic effects, however their use is associated with the broad spectrum of side effects observed in human as well as the experimental animals. despite damaging activity of nsaids in upper gastrointestinal (gi) tract, these drugs exert deleterious influence in lower gi tract, including colon. the role of gi microflora in the pathogenesis of nsaids-induced experimental colonic damage is n ... | 2011 | 21893697 |
| a novel cold-adapted lipase from sorangium cellulosum strain so0157-2: gene cloning, expression, and enzymatic characterization. | genome sequencing of cellulolytic myxobacterium sorangium cellulosum reveals many open-reading frames (orfs) encoding various degradation enzymes with low sequence similarity to those reported, but none of them has been characterized. in this paper, a predicted lipase gene (lipa) was cloned from s. cellulosum strain so0157-2 and characterized. lipa is 981-bp in size, encoding a polypeptide of 326 amino acids that contains the pentapeptide (ghsmg) and catalytic triad residues (ser114, asp250 and ... | 2011 | 22072918 |
| generation and characterization of a live attenuated enterotoxigenic escherichia coli combination vaccine expressing six colonization factors and heat-labile toxin subunit b. | live attenuated oral enterotoxigenic escherichia coli (etec) vaccines have been demonstrated to be safe and immunogenic in human volunteers and to provide a viable approach to provide protection against this important pathogen. this report describes the construction of new etec vaccine candidate strains from recent clinical isolates and their characterization. all known genes for etec toxins were removed, and attenuating deletion mutations were made in the aroc, ompc, and ompf chromosomal genes. ... | 2011 | 21994355 |
| synthesis, antimicrobial and antiproliferative activity of novel silver(i) tris(pyrazolyl)methanesulfonate and 1,3,5-triaza-7-phosphadamantane complexes. | five new silver(i) complexes of formulas [ag(tpms)] (1), [ag(tpms)(pph(3))] (2), [ag(tpms)(pcy(3))] (3), [ag(pta)][bf(4)] (4), and [ag(tpms)(pta)] (5) {tpms = tris(pyrazol-1-yl)methanesulfonate, pph(3) = triphenylphosphane, pcy(3) = tricyclohexylphosphane, pta = 1,3,5-triaza-7-phosphaadamantane} have been synthesized and fully characterized by elemental analyses, (1)h, (13)c, and (31)p nmr, electrospray ionization mass spectrometry (esi-ms), and ir spectroscopic techniques. the single crystal x- ... | 2011 | 21999582 |
| contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria escherichia coli. | the molecular mechanisms that define asymptomatic bacteriuria (abu) escherichia coli colonization of the human urinary tract remain to be properly elucidated. here, we utilize abu e. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness, and colonization of the urinary tract. we show that e. coli 83972 produces enterobactin, salmochelin, aerobactin, and yersiniabactin and examine the role of these systems using mutants defective in sideroph ... | 2012 | 21930757 |
| triazacryptand-based fluorescent sensors for extracellular and intracellular k+ sensing. | a 4-amino-naphthalimide derived fluorophore with a triazacryptand moiety ligand was synthesized as a potassium ion (k(+)) sensor (ks1). this sensor is a monomer possessing a polymerizable vinyl group. by taking advantage of the polymerizable characteristics of the vinyl group, ks1 was polymerized with 2-hydroxyethyl methacrylate (hema) and acrylamide (am) to form k(+) sensing films for extracellular sensing. the sensitivity of the films to potassium ions can be further tuned through the adjustme ... | 2011 | 21855134 |
| is escherichia coli urinary tract infection a zoonosis? proof of direct link with production animals and meat. | recently, it has been suggested that the escherichia coli causing urinary tract infection (uti) may come from meat and animals. the purpose was to investigate if a clonal link existed between e. coli from animals, meat and uti patients. twenty-two geographically and temporally matched b2 e. coli from uti patients, community-dwelling humans, broiler chicken meat, pork, and broiler chicken, previously identified to exhibit eight virulence genotypes by microarray-detection of approximately 300 gene ... | 2011 | 22033854 |
| [isolation of expressed in e. coli human interferon beta1b (ser17) by ion-exchange chromatography]. | a method for isolation of interferon beta1b (serl7) from inclusion bodies, comprising the steps of solution and reduction of protein from the inclusion bodies, refolding, chromatography on deae-sepharose, chromatography on sp-sepharose, concentrating, desalting and addition of stabilizers. the solution of reduced protein was diluted with ph 8.0 buffer of 50 mm tris-hcl, 25 microm cucl2 and 0.5% twin 20 for refolding. we used gradient of ph (from 9.3 upto 11.3) for elution of interferon-beta from ... | 2011 | 21899047 |
| antimicrobial evaluation of huilliche plant medicine used to treat wounds. | the traditional use of 40 plant species used for treatment of wounds and associated infections by the huilliche people of chile was evaluated against bacterial and fungal human pathogens, especially including wound pathogens. | 2011 | 21939748 |
| Suppression of adaptive immunity to heterologous antigens by Sj16 of Schistosoma japonicum. | Abstract Despite great effort has been given to control the disease, schistosomiasis remains the most important human helminth infection in terms of morbidity and mortality. Natural infection of schistosomes induces very little protective immunity against reinfection. Moreover, effective schistosomiasis vaccines for practical use have not been developed. Schistosomes appear to have evolved highly effective modulatory mechanisms on their host's immune system that promote the parasites' survival ... | 2011 | 22017401 |
| comparative genomics of multidrug resistance-encoding inca/c plasmids from commensal and pathogenic escherichia coli from multiple animal sources. | incompatibility group a/c (inca/c) plasmids have received recent attention for their broad host range and ability to confer resistance to multiple antimicrobial agents. due to the potential spread of multidrug resistance (mdr) phenotypes from foodborne pathogens to human pathogens, the dissemination of these plasmids represents a public health risk. in this study, four animal-source inca/c plasmids isolated from escherichia coli were sequenced and analyzed, including isolates from commercial dai ... | 2011 | 21858108 |
| Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorragic Escherichia coli infections. | Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the development of the infection, but the mechanisms involved remain unclear. Many bacterial pathogens target the follicle-associated epithelium, which overlies Peyer's patches (PPs), cross the intestinal barrier ... | 2011 | 21858177 |
| [neuroprotection of a neotype transactivating-brain-derived neurotrophic factor fusion protein with penetrating activity of blood-brain barrier]. | to identify the dural activities of neuroprotection and penetrating blood-brain barrier (bbb) for tat-bdnf (transactivating-brain-derived neurotrophic factor) fusion protein to explore an alternative treatment for the injury of central nerve system (cns). | 2011 | 22093740 |
| Carriage of Shiga-toxigenic Escherichia coli by native marsupials in Australia. | Shiga-toxigenic strains of Escherichia coli (STEC) are zoonotic pathogens with human health, meat processing and trade impacts. Cattle are the principal reservoirs of STEC, although other animals can be carriers. The STEC status of Australian native marsupials has not been formatively described to date. The aim of the current study was to investigate carriage of STEC by native Australian marsupials in Southeast Queensland. Faeces from a variety of marsupial species, stratified by gastrointestina ... | 2011 | 21917387 |
| Reprint of: Expression and purification of ataxin-1 protein. | Ataxin-1 is part of a larger family of polyglutamine-containing proteins that is linked to nine distinct neurodegenerative disorders. There are no known effective therapies for any of these expanded polyglutamine tract disorders. One possible reason for this is the lack of sufficient amounts of pure polyglutamine-containing proteins suitable for biochemical and conformational studies. Here, we show that we were able to successfully purify a non-pathological, wild-type human ataxin-1 protein cont ... | 2011 | 21893199 |
| the characterization and evolutionary relationships of a trypanosomal thiolase. | thiolases are enzymes that remove an acetyl-coenzyme a group from acyl-coa in the catabolic β-oxidation of fatty acids, or catalyse the reverse condensation reaction for anabolic processes such as the biosynthesis of sterols and ketone bodies. in humans, six homologous isoforms of thiolase have been described, differing from each other in sequence, oligomeric state, substrate specificity and subcellular localization. a bioinformatics analysis of parasite genomes, being (i) different species of a ... | 2011 | 21907205 |
| Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. | This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of Cassia fistula Linn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important ba ... | 2011 | 22171301 |
| microbiological contamination of digested products from anaerobic co-digestion of bovine manure and agricultural by-products. | this study was performed to investigate the microbiological contamination of digestate product (dp) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. | 2011 | 21899580 |
| Large-scale preparation and characterization of non-pegylated and pegylated superactive ovine leptin antagonist. | Superactive ovine leptin antagonist (SOLA) was prepared by rational mutagenesis of the ovine leptin antagonist L39A/D40A/F41A mutant prepared previously in our lab by mutating wild type leptin to D23L/L39A/D40A/F41A. SOLA was expressed in Escherichia coli as insoluble inclusion bodies, refolded and purified to homogeneity (as evidenced by SDS-PAGE and analytical gel filtration) by ion-exchange chromatography. The purified protein was mono-pegylated at its N terminus by 20-kDa linear pegylation r ... | 2012 | 22040607 |
| Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis. | Reversible phosphorylation of proteins is a critical mechanism involved in physiological function of organisms, including Clonorchis sinensis. In the present study, One cDNA clone encoding protein phosphatase 2A (CsPP2A) was isolated from a C. sinensis adult cDNA plasmid library. The open reading frame of the novel gene contains 924 bp and encoded a putative protein of 307 amino acids. A similarity analysis showed high homology with Schistosoma japonicum (76.3%) and Homo sapiens (84.4%), respect ... | 2011 | 22167368 |
| lps from neisseria meningitidis is crucial for inducing monocyte- and microparticle-associated tissue factor activity but not for tissue factor expression. | neisseria meningitidis causes sepsis with coagulopathy. the present study evaluated the tissue factor (tf)-inducing capacity of bacterial lps in different presentation forms, i.e. membrane-bound lps versus purified lps, and of non-lps components of n. meningitidis. by using a wild-type n. meningitidis, a mutant n. meningitidis lacking lps (lps-deficient n. meningitidis), purified lps from n. meningitidis and escherichia coli, we measured tf-expression and tf-activity on human monocytes and micro ... | 2011 | 22180561 |
| comparative analysis of espf variants in inhibition of escherichia coli phagocytosis by macrophages and inhibition of e. coli translocation through human- and bovine-derived m cells. | the espf protein is secreted by the type iii secretion system of enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively). espf sequences differ between ehec o157:h7, ehec o26:h11, and epec o127:h6 in terms of the number of sh3-binding polyproline-rich repeats and specific residues in these regions, as well as residues in the amino domain involved in cellular localization. espf(o127) is important for the inhibition of phagocytosis by epec and also limits epec translo ... | 2011 | 21875965 |
| genomic anatomy of escherichia coli o157:h7 outbreaks. | the rapid emergence of escherichia coli o157:h7 from an unknown strain in 1982 to the dominant hemorrhagic e. coli serotype in the united states and the cause of widespread outbreaks of human food-borne illness highlights a need to evaluate critically the extent to which genomic plasticity of this important enteric pathogen contributes to its pathogenic potential and its evolution as well as its adaptation in different ecological niches. aimed at a better understanding of the evolution of the e. ... | 2011 | 22135463 |
| bacteriophages carrying shiga toxin genes: genomic variations, detection and potential treatment of pathogenic bacteria. | although most escherichia coli strains occur in the mammalian intestine as commensals, some of them, including enterohemorrhagic e. coli (ehec), are capable of causing disease in humans. the most notorious virulence factors of ehec are shiga toxins, encoded by genes located on genomes of lambdoid prophages. production and release of these toxins is strongly stimulated after the induction of these prophages. many antibiotics used to treat bacterial infections stimulate induction of shiga toxin-co ... | 2011 | 21861621 |
| function and structure studies of gh family 31 and 97 α-glycosidases. | a huge number of glycoside hydrolases are classified into the glycoside hydrolase family (gh family) based on their amino-acid sequence similarity. the glycoside hydrolases acting on α-glucosidic linkage are in gh family 4, 13, 15, 31, 63, 97, and 122. this review deals mainly with findings on gh family 31 and 97 enzymes. research on two gh family 31 enzymes is described: clarification of the substrate recognition of escherichia coli α-xylosidase, and glycosynthase derived from schizosaccharomyc ... | 2011 | 22146713 |
| Mainstreams of horizontal gene exchange in enterobacteria: consideration of the outbreak of enterohemorrhagic E. coli O104:H4 in Germany in 2011. | Escherichia coli O104:H4 caused a severe outbreak in Europe in 2011. The strain TY-2482 sequenced from this outbreak allowed the discovery of its closest relatives but failed to resolve ways in which it originated and evolved. On account of the previous statement, may we expect similar upcoming outbreaks to occur recurrently or spontaneously in the future? The inability to answer these questions shows limitations of the current comparative and evolutionary genomics methods. | 2011 | 22022434 |
| interaction of endothelial progenitor cells expressing cytosine deaminase in tumor tissues and 5-fluorocytosine administration suppresses growth of 5-fluorouracil-sensitive liver cancer in mice. | the drug delivery system to tumors is a critical factor in upregulating the effect of anticancer drugs and reducing adverse events. recent studies indicated selective migration of bone marrow-derived endothelial progenitor cells (epc) into tumor tissues. cytosine deaminase (cd) transforms nontoxic 5-fluorocytosine (5-fc) into the highly toxic 5-fluorouracil (5-fu). we investigated the antitumor effect of a new cd/5-fc system with cd cdna transfected epc for hepatocellular carcinoma (hcc) in mice ... | 2011 | 22151662 |
| dna, protein binding, cytotoxicity, cellular uptake and antibacterial activities of new palladium(ii) complexes of thiosemicarbazone ligands: effects of substitution on biological activity. | the coordination propensities of 4(n,n')-diethylaminosalicylaldehyde-4(n)-substituted thiosemicarbazones (h(2)l(1-4)) were investigated by reacting with an equimolar amount of [pdcl(2)(pph(3))(2)]. the new complexes were characterized by various spectroscopic techniques. the structure determination of the complexes [pd(deasal-tsc)(pph(3))] (1), [pd(deasal-mtsc)(pph(3))] (2) and [pd(deasal-etsc)(pph(3))] (3) by x-ray crystallography showed that ligands are coordinated in a dibasic tridentate ons ... | 2011 | 22051854 |
| self-assembled, cation-selective ion channels from an oligo(ethylene glycol) derivative of benzothiazole aniline. | this paper describes the spontaneous formation of well-defined pores in planar lipid bilayers from the self-assembly of a small synthetic molecule that contains a benzothiazole aniline (bta) group attached to a tetra-ethylene glycol (eg4) moiety. macroscopic and single-channel current recordings suggest that these pores are formed by the assembly of four bta-eg4 monomers with an open pore diameter that appears similar to the one of gramicidin pores (~0.4 nm). the single-channel conductance of th ... | 2011 | 21889925 |
| European starlings (Sturnus vulgaris) challenged with Escherichia coli O157 can carry and transmit the human pathogen to cattle. | European starlings (Sturnus vulgaris) are an invasive species in the United States and are considered a nuisance pest to agriculture. The goal of this study was to determine the potential for these birds to be reservoirs and/or vectors for the human pathogen Escherichia coli O157:H7. | 2011 | 21985308 |
| polyclonal antibody against conserved sequences of mce1a protein blocks mtb infection in macrophages. | the pathogenesis of mycobacterium tuberculosis is largely due to its ability to enter and survive within human macrophages. it is suggested that a specific protein namely mammalian cell entry protein is involved in the pathogenesis and the specific gene for this protein mce1a has been identified in several pathogenic organisms such as rickettsia, shigella, escherichia coli, helicobacter, streptomyces, klebsiella, vibrio, neisseria, rhodococcus, nocardioides, saccharopolyspora erthyrae, and pseud ... | 2011 | 22159737 |
| crystallization and preliminary x-ray crystallographic study of the human mst2 sarah domain. | the sarah domain at the c-terminus of human mst2 (residues 436-484) was overproduced and purified using an escherichia coli expression system. the purified domain was crystallized using the hanging-drop vapour-diffusion technique. two crystal forms were obtained. the crystals belonged to space group p2, with unit-cell parameters a = 62.0, b = 119.2, c = 62.0 å, α = 90.0, β = 90.5, γ = 90.0°, or to space group p6(1)22, with unit-cell parameters a = 54.5, b = 54.5, c = 303.1 å. these crystals diff ... | 2011 | 22102242 |
| split-protein systems: beyond binary protein-protein interactions. | it has been estimated that 650,000 protein-protein interactions exist in the human interactome (stumpf et al., 2008), a subset of all possible macromolecular partnerships that dictate life. thus there is a continued need for the development of sensitive and user-friendly methods for cataloguing biomacromolecules in complex environments and for detecting their interactions, modifications, and cellular location. such methods also allow for establishing differences in the interactome between a norm ... | 2011 | 22070901 |
| hle-inhibitory alkaloids with a polyketide skeleton from the marine-derived fungus coniothyrium cereale. | the marine endophytic fungus coniothyrium cereale produces the structurally unusual polyketide-type alkaloids (-)-cereolactam (1) and (-)-cereoaldomine (3), incorporating a lactam and an imine functionality, respectively, as well as the related metabolite (-)-trypethelone (2). compounds 1 and 3 showed selective inhibition of human leukocyte elastase with ic50 values of 9.28 and 3.01 μm, respectively. compound 2 was found to be inhibitory toward mycobacterium phlei, staphylococcus aureus, and esc ... | 2011 | 21923104 |
| Bacterial superantigens enhance the in vitro proinflammatory response and in vivo lethality of the TLR2 agonist bacterial lipoprotein. | Bacterial superantigens are Gram-positive exotoxins that induce proinflammatory cytokine release in vitro, cause lethal shock in vivo, and can be detected in the bloodstream of critically ill patients. They also have a powerful priming effect on the TLR4 agonist LPS. The aim of this study was to investigate the relationship between superantigens and the TLR2 agonist bacterial lipoprotein (BLP). Priming of human monocytes or PBMCs with superantigens significantly enhanced proinflammatory cytokine ... | 2011 | 22003201 |
| a perspective on the prevalence of dna enteric virus genomes in anaerobic-digested biological wastes. | the major goal of this study is to gain a perspective on the prevalence of dna enteric virus genomes in mesophilic anaerobic-digested (mad) sewage sludge and manure by comparing their quantitative pcr (qpcr) concentrations and removals with traditional fecal indicators (escherichia coli, enterococci, and bacteroidetes). in addition, relationships between qpcr and culture measurements of fecal indicators (fis) were determined. there was no significant difference between the qpcr concentrations of ... | 2011 | 21931949 |
| Profile of Shiga toxin-producing Escherichia coli strains isolated from dogs and cats and genetic relationships with isolates from cattle, meat and humans. | Pets can be reservoirs of Shiga toxin-producing Escherichia coli (STEC) strains. The aim of this study was to examine nine strains belonging to several serotypes (O91:H21, O91:H16, O178:H19, O8:H19, O22:H8, O22:HNT, ONT:H8), previously recovered from cats or dogs. To this end, we assessed a set of additional virulence genes (stx(2) subtype, subAB, ehxA, eae and saa), cytotoxic activity, and genetic relationships with strains isolated from cattle, meat and humans using pulsed-field gel electropho ... | 2011 | 22119188 |
| a disulfide-free single-domain v(l) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition. | we present the crystal structure and biophysical characterization of a human v(l) [variable domain immunoglobulin (ig) light chain] single-domain intrabody that binds to the huntingtin (htt) protein and has been engineered for antigen recognition in the absence of its intradomain disulfide bond, otherwise conserved in the ig fold. analytical ultracentrifugation demonstrated that the αhtt-v(l) 12.3 domain is a stable monomer under physiological conditions even at concentrations >20 μm. using pept ... | 2011 | 21968397 |
| Innate Transcriptional Networks Activated in Bladder in Response to Uropathogenic Escherichia coli Drive Diverse Biological Pathways and Rapid Synthesis of IL-10 for Defense against Bacterial Urinary Tract Infection. | Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. In this study, we describe the whole bladder transcriptome of uropathogenic Escherichia coli (UPEC) cystitis in mice using genome-wide expression profiling to define the transcriptome of innate immune activation stemming from UPEC colonization of the bladder. Bladder RNA from female C57BL/6 mice, analyzed using 1.0 ST-Affymetrix microarrays, revealed e ... | 2011 | 22184725 |
| a potential test system for detecting contaminations by bacterial lipoproteins. | biological specimens are often contaminated with bacteria-derived products such as lps or lipoproteins (lp), which trigger unwanted inflammatory responses in hosts. whereas a contamination by lps can be determined by various test systems, a contamination by lp can as yet not be determined. tlr4 and tlr2 are key components of the lps and the lp receptor complex, respectively. it was tested in this study whether hek293 cell stably transfected with bovine tlr2 have the ability to react to low conce ... | 2011 | 22133281 |
| A new recombinant human apolipoprotein E mimetic peptide with high-density lipoprotein binding and function enhancing activity. | We generated a novel human apolipoprotein E (apoE)-mimetic peptide, designated EpK. EpK contains an N-terminal cysteine residue, a low-density lipoprotein receptor-binding fragment, a 6 × lysine linker and a lipid-binding fragment. The recombinant peptide was expressed in Escherichia coli, and purified with a chitin bead column followed by a Heparin Sepharose CL-6B column to yield pure peptide. EpK displayed high solubility in aqueous solution at neutral pH and adopted a low content of a-helical ... | 2011 | 22087021 |
| bactericidal activity of musca domestica cecropin (mdc) on multidrug-resistant clinical isolate of escherichia coli. | the housefly (musca domestica) larvae have been used clinically to cure osteomyelitis, decubital necrosis, lip boil, ecthyma and malnutritional stagnation ever since the ming/qing dynasty (1368 anno domini) till now, in china. in prior research, we have cloned and characterized a new gene of antimicrobial peptide cecropin from m. domestica larvae. this peptide was potently active against gram-positive and gram-negative bacteria standard strain. in the present study, we evaluated the possibility ... | 2011 | 22202966 |
| safety evaluation of a natural eggshell membrane-derived product. | natural eggshell membrane (nem®) is a novel dietary ingredient that contains naturally occurring glycosaminoglycans and proteins essential for maintaining healthy joint and connective tissues. nem® was evaluated for safety via in vitro and in vivo toxicological studies. this included testing for cytotoxicity, genotoxicity, acute oral toxicity, and 90-day repeated-dose oral toxicity. nem® did not exhibit any cytotoxic effects at a dose of 100μg in an in vitro human cell viability assay after incu ... | 2012 | 22245377 |
| posttransplant lymphoproliferative disorder presenting as multiple cystic lesions in a renal transplant recipient. | we report a case of a 67-year-old man who experienced allograft dysfunction following a renal transplantation from a donation after cardiac death. the postoperative course was initially complicated by episodes of e. coli urinary sepsis causing pyrexia and a raised creatinine level. ultrasound scanning 5 weeks posttransplant revealed mild hydronephrosis with several parenchymal cystic areas measuring up to 2 cm with appearances suggestive of fungal balls. aspirated fluid again grew escherichia co ... | 2012 | 22244123 |
| overexpression of potassium channel ether à go-go in human osteosarcoma. | human ether à go-go (heag) potassium channels are primarily expressed in brain but also frequently overexpressed in solid tumors, which could indicate their potential value for cancer diagnosis and therapy. heag1, one member of the heag subfamily, has been shown to play a role in neoplastic process. here we report the expression of heag1 in human osteosarcoma detected by a new polyclonal antibody. the full-length heag1 cdna was cloned from human osteosarcoma cell line mg63 by rt-pcr and expresse ... | 2012 | 22248279 |
| escherichia coli-induced epithelial hyporesponsiveness to secretagogues is associated with altered cftr localization. | both pathogenic and commensal strains of escherichia coli colonize the human intestinal tract. pathogenic strains differ only in the expression of virulence factors, many of which comprise a type iii secretion system (ttss). little is known regarding the effect of e. coli on the intestinal epithelial response to the secretagogues that drive ion secretion, despite its importance in causing clinically significant diarrhea. using ussing chambers to measure electrogenic ion transport of t84 intestin ... | 2011 | 22212348 |
| [preparation and identification of recombinant human neutrophil elastase]. | to prepare a purified recombinant human neutrophil elastase (hne) using genetic engineering technology, and pave the way for the preparation of the antibody to hne and establishment of semen hne detection methods. | 2011 | 22235674 |
| incidence and virulence determinants of verocytotoxin-producing escherichia coli infections in brussels-capital region, belgium, 2008-2010. | the incidence of verocytotoxin-producing escherichia coli (vtec) was investigated by pcr in all human stools from universitair ziekenhuis brussel (uzb) and selected stools from six other hospital laboratories in brussels-capital region (belgium) collected between april 2008 and october 2010. selected stools to be included in this study were those from patients with hemolytic uremic syndrome (hus), patients with a history of bloody diarrhea, patients linked to clusters of diarrhea, children up to ... | 2012 | 22238434 |