| identification of active constraints in dynamic flux balance analysis. | this study deals with the calibration of dynamic metabolic flux models that are formulated as the maximization of an objective subject to constraints. two approaches were applied for identifying the constraints from data. in the first approach a minimal active number of limiting constraints is found based on data that are assumed to be bounded within sets whereas, in the second approach, the limiting constraints are found based on parametric sensitivity analysis. the ability of these approaches ... | 2017 | 27790866 |
| immunogenicity and protective efficacy of recombinant iron superoxide dismutase protein from bordetella pertussis in mice models. | whooping cough (pertussis) is a highly contagious respiratory infection caused by bordetella pertussis. although availability of effective pertussis vaccines reportedly decreases the incidence of the disease, b. pertussis circulation in populations has not been eliminated. thus, it is necessary to find new protein candidates with greater immune protective capacities than the currently available acellular pertussis vaccines. in this study, iron superoxide dismutase (fesod) gene (sodb) was cloned, ... | 2016 | 27761933 |
| pertussis outbreak among patients and healthcare workers in a provincial dialysis facility in japan. | sixteen pertussis cases in haemodialysis patients and healthcare workers were detected in a 25-bed outpatient haemodialysis facility in japan between october 2013 and april 2014. | 2016 | 27760708 |
| the pertussis hypothesis: bordetella pertussis colonization in the pathogenesis of alzheimer's disease. | while a number of endogenous risk factors including age and genetics are established for alzheimer's disease (ad), identification of acquired, potentially preventable or treatable causes, remains limited. in this paper, we review three epidemiologic case studies and present extensive biologic, immunologic and anatomic evidence to support a novel hypothesis that bordetella pertussis (bp), the bacterium better known to cause whooping cough, is an important potential cause of ad. cross-cultural doc ... | 2017 | 27692981 |
| randomized clinical trial of the safety and immunogenicity of the tdap vaccine in pregnant mexican women. | immunization with the tetanus, diphtheria, and pertussis (tdap) vaccine raises controversies on immunogenicity and possible antibody interference. we performed an experimental, double-blind, parallel group controlled clinical trial to evaluate the safety and immunogenicity of the tdap vaccine in 204 pregnant women and their children and to determine its interference in antibody production. pregnant women 18 to 38 y of age with 12 to 24 weeks gestation, a low obstetric risk, and without serious d ... | 2017 | 27686182 |
| biofilm forming potential and antimicrobial susceptibility of newly emerged western australian bordetella pertussis clinical isolates. | whooping cough caused by bordetella pertussis is increasing in several countries despite high vaccine coverage. one potential reason for the resurgence is the emergence of genetic variants of the bacterium. biofilm formation has recently been associated with the pathogenesis of b. pertussis. biofilm formation of 21 western australian b. pertussis clinical isolates was investigated. all isolates formed thicker biofilms than the reference vaccine strain tohama i while retaining susceptibility to a ... | 2016 | 27669900 |
| prior exposure to bordetella species as an exclusion criterion in the baboon model of pertussis. | the baboon model of bordetella pertussis infection is the newest and most clinically accurate model of the human disease to date. however, among the 15 experimentally infected baboons in this study, a subset of baboons did not exhibit the expected high bacterial colonization levels or increase in white blood cell count. moreover, cultures of nasopharyngeal wash samples from several baboons suggested b. bronchiseptica coinfection. analysis of serum antibodies recognizing filamentous hemagglutinin ... | 2017 | 27666464 |
| highlights of the 11th international bordetella symposium: from basic biology to vaccine development. | pertussis is a severe respiratory disease caused by infection with the bacterial pathogen bordetella pertussis the disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. other bordetella species cause diseases in humans, animals, and birds. scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in buenos aires, argentina from 5 to 8 april 2016 for the 11th internat ... | 2016 | 27655886 |
| clinical manifestations and molecular characterization of pertactin-deficient and pertactin-producing bordetella pertussis in children, philadelphia 2007-2014. | bordetella pertussis strains lacking expression of pertactin, a bacterial adhesin and vaccine target, are emerging. there are limited data on disease manifestations of mutant strains in children. we sought to compare clinical manifestations of pertactin-deficient and pertactin-producing b. pertussis infection in infants and describe corresponding molecular characteristics. | 2017 | 27624959 |
| globin domain interactions control heme pocket conformation and oligomerization of globin coupled sensors. | globin coupled sensors (gcs) are o2-sensing proteins used by bacteria to monitor the surrounding gaseous environment. to investigate the biphasic o2 dissociation kinetics observed for full-length gcs proteins, isolated globin domains from pectobacterium carotovorum ssp. carotovorum (pccglobin), and bordetella pertussis (bpeglobin), have been characterized. pccglobin is found to be dimeric, while bpeglobin is monomeric, indicating key differences in the globin domain dimer interface. through char ... | 2016 | 27614715 |
| pertussis leukocytosis: mechanisms, clinical relevance and treatment. | the significant and sometimes dramatic rise in the number of circulating white blood cells (leukocytosis) in infants suffering from pertussis (whooping cough) has been recognized for over a century. although pertussis is a disease that afflicts people of all ages, it can be particularly severe in young infants, and these are the individuals in whom leukocytosis is most pronounced. very high levels of leukocytosis are associated with poor outcome in infants hospitalized with pertussis and modern ... | 2016 | 27609461 |
| synergistic neutralization of pertussis toxin by a bispecific antibody in vitro and in vivo. | bispecific antibodies are a rapidly growing class of therapeutic molecules, originally developed for the treatment of cancer but recently explored for the treatment of autoimmune and infectious diseases. bordetella pertussis is a reemerging pathogen, and several of the key symptoms of infection are caused by the pertussis toxin (ptx). two humanized antibodies, hu1b7 and hu11e6, bind distinct epitopes on ptx and, when coadministered, mitigate disease severity in murine and baboon models of infect ... | 2016 | 27581436 |
| [insertional inactivation of virulence operon in population of persistent bordetella pertussis bacteria]. | avirulent b. pertussis bacteria containing is elements in the bvgas operon were detected during the study of whooping cough patients and bacilli carriers. the present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the b. pertussis bacteria in lower monkeys as the most adequate model for extrapolation ofthe experiment results to humans. by means of the real-time pcr method, it was established that the b. pertussis bacteria lived m ... | 2016 | 27529975 |
| [serological evaluation of bordetella pertussis infection in adults with prolonged cough]. | pertussis is a vaccine-preventable disease that is transmitted from infected to susceptible individuals by respiratory route. bordetella pertussis infection may occur at any age as neither vaccine nor natural infection induced immunity lasts life-long. this study was planned to demonstrate the serological evidence of infection among adults, to raise awareness among clinicians and to provide data for the development of strategies to protect vulnerable infants. a total of 538 patients (345 female, ... | 2016 | 27525392 |
| viral etiology in adult influenza-like illness/acute respiratory infection and predictivity of c-reactive protein. | influenza-like illness (ili) and acute respiratory infection (ari) are common presentations during winter and indiscriminate antibiotic use contributes significantly to the emerging post-antibiotic era. | 2016 | 27482806 |
| [multiple-locus variable number tandem repeat analysis of bordetella pertussis strains collected in the czech republic in 1967-2015: spread of a variant adapted to the population with a high vaccination coverage]. | to perform multiple-locus variable number tandem repeat analysis (mlva) of b. pertussis strains from the collection of the national reference laboratory for diphtheria and pertussis (nrl/dipe), national institute of public health (niph), prague. the study strains were isolated from clinical specimens collected mostly in the czech republic over a nearly 50-year period from 1967 to 2015 (june). the isolates from three periods characterized by different vaccination strategies and trends in pertussi ... | 2017 | 27467327 |
| [novel approaches to control the rise in pertussis cases]. | pertussis is a respiratory disease caused by the gram-negative encapsulated bacterium bordetella pertussis. despite the high vaccination coverage rate and addition of new booster doses to the immunisation scheme (in response to the epidemiological situation), pertussis is on the rise not only in the czech republic but also in many other countries. the age groups at highest risk are infants and, to a lower extent, newborns who can get infected before receiving the first dose of vaccine and develo ... | 2017 | 27467322 |
| bordetella pertussis modulates human macrophage defense gene expression. | bordetella pertussis, the etiological agent of whooping cough, still causes outbreaks. we recently found evidence that b. pertussis can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. in this work, we examined the interaction of b. pertussis with a human monocyte cell line (thp-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promot ... | 2016 | 27465637 |
| adaptive immune response to whole cell pertussis vaccine reflects vaccine quality: a possible complementation to the pertussis serological potency test. | whole cell bordetella pertussis (wp) vaccines are still used in many countries to protect against the respiratory disease pertussis. the potency of whole-cell pertussis vaccine lots is determined by an intracerebral challenge test (the kendrick test). this test is criticized due to lack of immunological relevance of the read-out after an intracerebral challenge with b. pertussis. the alternative in vivo test, which assesses specific antibody levels in serum after wp vaccination, is the pertussis ... | 2016 | 27452867 |
| evaluation of commercial assays for single-point diagnosis of pertussis in the us. | pertussis serodiagnosis is increasingly being used in the united states despite the lack of a us food and drug administration-approved, commercially available assay. to better understand the utility of these assays in diagnosing pertussis, serology assays were evaluated for analytical parameters and clinical accuracy. | 2016 | 27451419 |
| vitamins as influenza vaccine adjuvant components. | a number of adjuvant formulations were assayed in mice immunized with 3.75 µg of a/california/7/2009 (h1n1) pdm09 influenza vaccine with vitamins a, d and/or e in emulsions or b2 and/or b9 combined with bordetella pertussis mpla and/or alum as adjuvants. squalene was used as positive control, as well as mpla with alum. the immune response was evaluated by a panel of tests, including a hemagglutination inhibition (hai) test, elisa for igg, igg1, and igg2a and ifn-γ, il-2, il-6 and il-10 quantific ... | 2016 | 27449155 |
| comparison of point-of-care-compatible lysis methods for bacteria and viruses. | nucleic acid sample preparation has been an especially challenging barrier to point-of-care nucleic acid amplification tests in low-resource settings. here we provide a head-to-head comparison of methods for lysis of, and nucleic acid release from, several pathogenic bacteria and viruses-methods that are adaptable to point-of-care usage in low-resource settings. digestion with achromopeptidase, a mixture of proteases and peptidoglycan-specific hydrolases, followed by thermal deactivation in a bo ... | 2016 | 27424294 |
| new horizons in the development of novel needle-free immunization strategies to increase vaccination efficacy. | the young twenty-first century has already brought several medical advances, such as a functional artificial human liver created from stem cells, improved antiviral (e.g., against hiv) and cancer (e.g., against breast cancer) therapies, interventions controlling cardiovascular diseases, and development of new and optimized vaccines (e.g., hpv vaccine). however, despite this substantial progress and the achievements of the last century, humans still suffer considerably from diseases, especially f ... | 2016 | 27370343 |
| intracellular disassembly and activity of pertussis toxin require interaction with atp. | the active subunit (s1) of pertussis toxin (pt), a major virulence factor of bordetella pertussis, adp-ribosylates gi proteins in the mammalian cell cytosol to inhibit gpcr signaling. the intracellular pathway of pt includes endocytosis and retrograde transport to the trans-golgi network (tgn) and endoplasmic reticulum (er). subsequent translocation of s1 to the cytosol is presumably preceded by dissociation from the holotoxin. in vitro, such dissociation is stimulated by interaction of pt with ... | 2016 | 27369899 |
| burden and outcomes of severe pertussis infection in critically ill infants. | despite world health organization endorsed immunization schedules, bordetella pertussis continues to cause severe infections, predominantly in infants. there is a lack of data on the frequency and outcome of severe pertussis infections in infants requiring icu admission. we aimed to describe admission rates, severity, mortality, and costs of pertussis infections in critically ill infants. | 2016 | 27362854 |
| better colonisation of newly emerged bordetella pertussis in the co-infection mouse model study. | molecular epidemiological data indicates that the resurgence of pertussis (whooping cough) in populations with high vaccine coverage is associated with genomic adaptation of bordetella pertussis, the causative agent of the disease, to vaccine selection pressure. we have previously shown that in the period after the introduction of acellular pertussis vaccine (acv), the majority of circulating strains in australia switched to single nucleotide polymorphism (snp) cluster i (carrying ptxp3/prn2), r ... | 2016 | 27346304 |
| purification design and practice for pertactin, the third component of acellular pertussis vaccine, from bordetella pertussis. | development of acellular pertussis vaccine (apv) requires purification of several components from bordetella pertussis. while the components pertussis toxin (pt) and filamentous hemagglutinin (fha) have been successfully purified, the third component, pertactin, proves to be a difficult target due to its very low concentration. in order to solve its purification problem, we performed the surface potential analysis with grasp2 program. the results demonstrated that there are two major charge patc ... | 2016 | 27302339 |
| pertussis: history of the disease and current prevention failure. | pertussis or whooping cough has been given many names over the centuries. it was first recognized in the middle ages and since then various epidemics have been described. jules bordet and octave gengou isolated bordetella pertussis, a causative agent for whooping cough, in paris more than 100 years ago, which created an excellent opportunity to invent a vaccine. in 1914 the whole-cell pertussis vaccine was invented, then in the 1940s it was combined with tetanus and diphtheria toxoids to become ... | 2016 | 27256351 |
| gtl001, a therapeutic vaccine for women infected with human papillomavirus 16 or 18 and normal cervical cytology: results of a phase i clinical trial. | women infected with human papillomavirus (hpv) with normal cytology to mild abnormalities currently have no treatment options other than watchful waiting or surgery if high-grade cervical lesions or cancer develop. a therapeutic vaccine would offer the possibility of preventing high-grade lesions in hpv-infected women. gtl001 is a therapeutic vaccine composed of recombinant hpv16 and hpv18 e7 proteins fused to catalytically inactive bordetella pertussis cyaa. this study examined the tolerability ... | 2016 | 27252412 |
| pertussis vaccine effectiveness in the setting of pertactin-deficient pertussis. | in the united states, the proportion of bordetella pertussis isolates lacking pertactin, a component of acellular pertussis vaccines, increased from 14% in 2010 to 85% in 2012. the impact on vaccine effectiveness (ve) is unknown. | 2016 | 27244813 |
| surveillance of pertussis: methods and implementation. | pertussis or whooping cough is a respiratory disease caused by bordetella pertussis or, to a lesser extent, by b. parapertussis. vaccines against pertussis have been widely used for more than 50 years and have led to a significant reduction of morbidity and mortality. however, even in countries with a high vaccine coverage, the disease is still not well controlled. surveillance is urgently needed. | 2016 | 27224518 |
| infectious disease report: bordetella pertussis infection in patients with cancer. | we illustrate 2 cases of pneumonia associated with bordetella pertussis infection in 72-year-old and 61-year-old patients with cancer receiving myelosuppressive therapy after hematopoietic stem cell transplantation. bacterial infections are a significant cause of morbidity and mortality in patients with cancer, and those receiving hematopoietic stem cell transplant, solid organ transplant, or myelosuppressive therapy are at increased risk. the infection was detected and the 2 patients had good o ... | 2016 | 27218794 |
| evolution of bordetella pertussis. | | 2016 | 27213264 |
| th1 versus th2 t cell polarization by whole-cell and acellular childhood pertussis vaccines persists upon re-immunization in adolescence and adulthood. | the recent increase in cases of whooping cough among teenagers in the us suggests that the acellular bordetella pertussis vaccine (ap) that became standard in the mid 1990s might be relatively less effective than the whole-bacteria formulation (wp) previously used since the 1950s. to understand this effect, we compared antibody and t cell responses to a booster immunization in subjects who received either the wp or ap vaccine as their initial priming dose in childhood. antibody responses in wp- ... | 2017 | 27212461 |
| pertussis: where did we go wrong and what can we do about it? | pertussis or whooping cough, mainly caused by the gram-negative coccobacillus bordetella pertussis, is a severe respiratory disease that can by life-threatening especially in young infants. it has recently made a spectacular come-back in high vaccination-coverage countries, such as the us, australia and many european countries. although a trend towards increased pertussis incidence was already visible before the switch from whole-cell to acellular vaccines, it was really since the introduction o ... | 2016 | 27161992 |
| characterization of the immune response induced by pertussis omvs-based vaccine. | for the development of a third generation of pertussis vaccine that could improve the control of the disease, it was proposed that the immune responses induced by the classic whole cell vaccine (wp) or after infection should be used as a reference point. we have recently identified a vaccine candidate based on outer membrane vesicles (omvs) derived from the disease etiologic agent that have been shown to be safe and protective in mice model of infection. here we characterized omvs-mediated immun ... | 2016 | 27151884 |
| direct molecular typing of bordetella pertussis from nasopharyngeal specimens in china in 2012-2013. | data on the molecular epidemiology of bordetella pertussis are limited in developing countries where whole-cell pertussis vaccines (wcvs) have been used. the aim of this study was to determine the genotypes of circulating b. pertussis in china by direct molecular typing of clinical specimens. dna extracts of 122 nasopharyngeal swabs (nps) positive for b. pertussis by polymerase chain reaction (pcr) (targeting is481 and ptx-pr) from 2012 to 2013 were used for typing using the multiple-locus varia ... | 2016 | 27146879 |
| immunization against pertussis in adolescents and adults. | pertussis is a highly contagious infectious disease caused by bordetella pertussis that can be extremely serious, particularly in young infants. for many years the efforts of health authorities throughout the world to prevent pertussis had the main goals of reducing the morbidity of infants and children under 5 years of age, maintaining protection for several years during the school-age period and developing a significant herd immunity to directly and indirectly reduce the risk of the spread of ... | 2016 | 27130670 |
| aetiology of childhood pneumonia in a well vaccinated south african birth cohort: a nested case-control study of the drakenstein child health study. | pneumonia is a leading cause of mortality and morbidity in children globally. the cause of pneumonia after introduction of the 13-valent pneumococcal conjugate vaccine (pcv13) has not been well studied in low-income and middle-income countries, and most data are from cross-sectional studies of children admitted to hospital. we aimed to longitudinally investigate the incidence and causes of childhood pneumonia in a south african birth cohort. | 2016 | 27117547 |
| serodiagnosis of whooping cough in belgium: results of the national reference centre for bordetella pertussis anno 2013. | report on the pitfalls of serodiagnosis of pertussis in belgium for 2013 by the nrc bordetella. | 2016 | 27075799 |
| evidence of increased circulation of bordetella pertussis in the italian adult population from seroprevalence data (2012-2013). | incidence data on pertussis cases in italy do not show pertussis resurgence, as recently described in other european countries. the aim of this study was to determine the seroprevalence of igg antibodies to pertussis toxin (pt-igg) in selected adult age groups, which can serve as a reservoir of b. pertussis and be responsible for onward transmission to vulnerable infants. the seroprevalence of pt-igg was studied in sera collected in 2012-13 in age groups 20-29, 30-39 (reproductive age), and ≥ 60 ... | 2016 | 27075481 |
| development of a gene delivery system in streptococcus gordonii using thymidylate synthase as a selection marker. | streptococcus gordonii, a commensal bacterium of the human oral cavity, is a potential live vaccine vector. in this study, we have developed a system that delivers a vaccine antigen gene onto the chromosome of s. gordonii. the system consisted of a recipient strain, that is a thymidine auxotroph constructed by deletion of a portion of thya gene, and a linear gene delivery construct, composed of the functional thya gene, the vaccine antigen gene, and a dna fragment immediately downstream of thya. ... | 2016 | 27062990 |
| calcium-driven folding of rtx domain β-rolls ratchets translocation of rtx proteins through type i secretion ducts. | calcium-binding rtx proteins are equipped with c-terminal secretion signals and translocate from the ca(2+)-depleted cytosol of gram-negative bacteria directly into the ca(2+)-rich external milieu, passing through the "channel-tunnel" ducts of type i secretion systems (t1sss). using bordetella pertussis adenylate cyclase toxin, we solved the structure of an essential c-terminal assembly that caps the rtx domains of rtx family leukotoxins. this is shown to scaffold directional ca(2+)-dependent fo ... | 2016 | 27058787 |
| pertussis: microbiology, disease, treatment, and prevention. | pertussis is a severe respiratory infection caused by bordetella pertussis, and in 2008, pertussis was associated with an estimated 16 million cases and 195,000 deaths globally. sizeable outbreaks of pertussis have been reported over the past 5 years, and disease reemergence has been the focus of international attention to develop a deeper understanding of pathogen virulence and genetic evolution of b. pertussis strains. during the past 20 years, the scientific community has recognized pertussis ... | 2016 | 27029594 |
| epidemiological and economic effects of priming with the whole-cell bordetella pertussis vaccine. | current acellular pertussis vaccines may not protect against transmission of bordetella pertussis. | 2016 | 27018830 |
| a novel igm-capture enzyme-linked immunosorbent assay using recombinant vag8 fusion protein for the accurate and early diagnosis of bordetella pertussis infection. | an elisa that measures anti-pt igg antibody has been used widely for the serodiagnosis of pertussis; however, the igg-based elisa is inadequate for patients during the acute phase of the disease because of the slow response of anti-pt igg antibodies. to solve this problem, we developed a novel igm-capture elisa that measures serum anti-bordetella pertussis vag8 igm levels for the accurate and early diagnosis of pertussis. first, we confirmed that vag8 was highly expressed in all b. pertussis iso ... | 2016 | 26996337 |
| effect of different detoxification procedures on the residual pertussis toxin activities in vaccines. | pertussis toxin (ptx) is a major virulence factor produced by bordetella pertussis and its detoxified form is one of the major protective antigens in vaccines against whooping cough. ideally, ptx in the vaccine should be completely detoxified while still preserving immunogenicity. however, this may not always be the case. due to multilevel reaction mechanisms of chemical detoxification that act on different molecular sites and with different production processes, it is difficult to define a mole ... | 2016 | 26973066 |
| the optimal gestation for pertussis vaccination during pregnancy: a prospective cohort study. | there is an increasing incidence of pertussis infection in infants too young to be adequately protected via vaccination. maternal pertussis vaccination during the third trimester of pregnancy is a new strategy to provide protection to newborn infants. | 2016 | 26968625 |
| [toward a new immunization schedule in spain, 2016 (part 2)]. | immunization schedules are intrinsically dynamic in order to embed the immunologic and epidemiologic changes in any specific geographic region. according to this, the current study addresses a proposal to modify the childhood immunization schedule in spain. in order to move from a three plus one schema to a two plus one, we undertake a review of the available literature to explore the immunological and clinical rationale behind this change, including an overview of the potential impact on this s ... | 2016 | 26960345 |
| [toward a new immunization schedule in spain, 2016 (part 1)]. | the immunization schedule is a dynamic public health tool that has incorporated different changes over the years influenced by the epidemiologic situation and the scientific evidence. the immunization advisory committee [ponencia de programa y registro de vacunaciones], as the interterritorial council scientific and technical advisory body, carries out assessments of different programmes and vaccines and proposes changes that after approval will be introduced in the regions schedule. this articl ... | 2016 | 26960253 |
| [persistence of bordetella pertussis bacteria and a possible mechanism of its formation]. | a growth of pertussis morbidity is observed in many countries of the world against the background of mass vaccindtion. forms of the disease course have changed. atypical forms of pertussis occur predominately in adolescents and adults. asymptomatic carriage of the causative agent has been established. infection of infants with. bordetelia pertussis bacteria in more than 90% of cases occurs from parents and relatives. a prolonged persistence of the causative agent has been identified. morbidity i ... | 2016 | 26951000 |
| bordetella pertussis epidemiology and evolution in the light of pertussis resurgence. | whooping cough, or pertussis, is resurgent in many countries world-wide. this is linked to switching from the use of whole cell vaccines to acellular vaccines in developed countries. current evidence suggests that this has resulted in the earlier waning of vaccine-induced immunity, an increase in asymptomatic infection with concomitant increases in transmission and increased selection pressure for bordetellapertussis variants that are better able to evade vaccine-mediated immunity than older iso ... | 2016 | 26932577 |
| comparison of the immunogenicity and safety of pentavalent vaccine quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus single-dose vials in healthy infants: a phase 3, open-label, randomized, parallel-group, non-inferiority study. | to evaluate non-inferiority of three doses of quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus quinvaxem in a single-dose vial administered with conventional syringe in terms of seroconversion/seroprotection rates for all antibodies (anti-hepatitis b (hb), anti-haemophilus influenzae type b polyribosylribitol phosphate (hib prp), anti-diphtheria, anti-tetanus, anti-bordetella pertussis) at 1 month after primary vaccination. | 2016 | 26923083 |
| antigenic and genetic characterization of bordetella pertussis recovered from quebec, canada, 2002-2014: detection of a genetic shift. | despite vaccination, cyclical peaks of bordetella pertussis incidence rates are still observed in canada and other developed countries, making pertussis one of the most prevalent vaccine preventable bacterial diseases. in the postacellular vaccine era, evolution of bacterial strains has resulted in strains with altered vaccine antigens. previous canadian studies have focused on isolates mainly from the provinces of ontario and alberta, with only small numbers of isolates from other provinces. th ... | 2016 | 26910633 |
| the role of b. pertussis vaccine antigen gene variants in pertussis resurgence and possible consequences for vaccine development. | whooping cough, or pertussis, caused by bordetella pertussis is considered resurgent in a number of countries world-wide, despite continued high level vaccine coverage. among a number of causes for this that have been proposed, is the emergence of b. pertussis strains expressing variants of the antigens contained in acellular pertussis vaccines; i.e. the evolution of b. pertussis toward vaccine escape. this commentary highlights the contradictory nature of evidence for this but also discusses th ... | 2016 | 26889694 |
| monitoring of an antigen manufacturing process. | fluorescence spectroscopy in combination with multivariate statistical methods was employed as a tool for monitoring the manufacturing process of pertactin (prn), one of the virulence factors of bordetella pertussis utilized in whopping cough vaccines. fluorophores such as amino acids and co-enzymes were detected throughout the process. the fluorescence data collected at different stages of the fermentation and purification process were treated employing principal component analysis (pca). throu ... | 2016 | 26879644 |
| proteome analysis of bordetella pertussis isolated from human macrophages. | previous studies have shown that b. pertussis survives inside human macrophages in non-acidic compartments with characteristics of early endosomes. in order to gain new insight into the biology of b. pertussis survival in host cells, we have analyzed the adaptation of the bacterial proteome during intracellular infection. the proteome of b. pertussis 3 h and 48 h after infection of human macrophage-like thp-1 cells was examined by nano-liquid chromatography combined with tandem ms and compared t ... | 2016 | 26873878 |
| porcelain lung: calcification in severe bordetella pertussis infection. | | 2016 | 26868038 |
| differences in bordetella pertussis dna load according to clinical and epidemiological characteristics of patients with whooping cough. | to identify associations between nasopharyngeal bordetella pertussis dna load and clinical and epidemiological characteristics and evaluate dna load prognostic value in pertussis severity. | 2016 | 26850358 |
| [pertussis (whooping cough)--an update]. | whooping cough is a highly contagious respiratory disease which is caused predominantly by the gram-negative bacterium bordetella pertussis. further bordetella species such as b. parapertussis and the recently discovered species b. holmesii are also involved in whooping cough-like diseases. depending on age, vaccination status and distance to pre-infection with b. pertussis, whooping cough shows a wide range of symptoms. the disease occurs at any age, leaving only short time immunity. during the ... | 2015 | 26837155 |
| understanding the true burden and infection dynamics of bordetella pertussis using molecular diagnostics. | | 2016 | 26826519 |
| genomic dissection of australian bordetella pertussis isolates from the 2008-2012 epidemic. | despite high pertussis vaccination coverage, australia experienced a prolonged epidemic in 2008-2012. the predominant bordetella pertussis genotype harboured pertussis toxin promoter allele, ptxp3, and pertactin gene allele, prn2. the emergence and expansion of prn non-expressing isolates (prn negative), were also observed. we aimed to investigate the microevolution and genomic diversity of epidemic b. pertussis isolates. | 2016 | 26826518 |
| whooping cough in a renal transplant recipient. | whooping cough is a respiratory infection with a severity that varies with age, immune status, and probably with other factors such as the degree of exposure and the virulence of the organism. the most frequent microorganism responsible for whooping cough is bordetella pertussis. we present the case of a 62-year-old renal transplant recipient presenting with typical and severe manifestations of whooping cough caused by b. pertussis. | 2016 | 26808962 |
| transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of bordetella adenylate cyclase toxin. | adenylate cyclase toxin-hemolysin (cyaa, act or ac-hly) of the whooping cough agent bordetella pertussis penetrates phagocytes expressing the integrin complement receptor 3 (cr3, cd11b/cd18, α(m)β(2) or mac-1). cyaa translocates its adenylate cyclase (ac) enzyme domain into cell cytosol and catalyzes unregulated conversion of atp to camp, thereby subverting cellular signaling. in parallel, cyaa forms small cation-selective membrane pores that permeabilize cells for potassium efflux, contributing ... | 2016 | 26802078 |
| reciprocal interference of maternal and infant immunization in protection against pertussis. | because of the current re-emergence of pertussis, vaccination during the 3rd trimester of pregnancy is recommended in several countries in order to protect neonates by placental transfer of maternal antibodies. here, we examined the potential reciprocal interference of mother and infant vaccination in protection against pertussis in mice. | 2016 | 26776471 |
| [clinical analysis of 247 children with whooping cough and the risk factors of severe cases]. | to summarize the clinical characteristics of whooping cough in children and analyze the risk factors for severe whooping cough. | 2015 | 26757969 |
| the potential role of subclinical bordetella pertussis colonization in the etiology of multiple sclerosis. | it is established that (1) subclinical bordetella pertussis colonization of the nasopharynx persists in highly vaccinated populations, and (2) b. pertussis toxin is a potent adjuvant that, when co-administered with neural antigens, induces neuropathology in experimental autoimmune encephalomyelitis, the principle animal model of multiple sclerosis. building on these observations with supporting epidemiologic and biologic evidence, we propose that, contrary to conventional wisdom that subclinical ... | 2016 | 26724970 |
| bordetella pertussis filamentous hemagglutinin itself does not trigger anti-inflammatory interleukin-10 production by human dendritic cells. | filamentous hemagglutinin (fha) is an important adhesin of the whooping cough agent bordetella pertussis and is contained in most acellular pertussis vaccines. recently, fha was proposed to exert an immunomodulatory activity through induction of tolerogenic il-10 secretion from dendritic cells. we have re-evaluated the cytokine-inducing activity of fha, placing specific emphasis on the role of the residual endotoxin contamination of fha preparations. we show that endotoxin depletion did not affe ... | 2016 | 26699834 |
| the relationship between bordetella pertussis genotype and clinical severity in australian children with pertussis. | changes in circulating bordetella pertussis genotypes, including a novel pertussis toxin promoter ptxp3 allele and absence of pertactin (prn) antigen, have been reported from several countries but limited data on relative severity are available. we compared markers of disease severity in children with b. pertussis infection due to strains of differing genotype. | 2016 | 26675318 |
| [pertussis vaccines: acellular versus whole cell. perhaps a return to the past?]. | the resurgence of pertussis in the world and in our country has questioned the effectiveness of cellular and acellular vaccines. the reason why pertussis has not been controlled or eliminated after 70 years of implementation of the vaccination is probably multifactorial. this article, on the basis of questions and answers, describes the benefits and limitations of both cellular and acellular vaccines and suggests new strategies of vaccination in childhood. it is a fact that the currently applied ... | 2015 | 26633113 |
| a cocktail of humanized anti-pertussis toxin antibodies limits disease in murine and baboon models of whooping cough. | despite widespread vaccination, pertussis rates are rising in industrialized countries and remain high worldwide. with no specific therapeutics to treat disease, pertussis continues to cause considerable infant morbidity and mortality. the pertussis toxin is a major contributor to disease, responsible for local and systemic effects including leukocytosis and immunosuppression. we humanized two murine monoclonal antibodies that neutralize pertussis toxin and expressed them as human immunoglobulin ... | 2015 | 26631634 |
| duration of bordetella pertussis polymerase chain reaction positivity in confirmed pertussis illness. | pertussis cases have been increasing in the united states for the past 40 years. polymerase chain reaction (pcr) testing is now the preferred method of diagnosis, but little is known about duration of pcr positivity. we conducted a pilot study using serial b pertussis pcr testing to determine duration of pcr positivity. kaplan-meier survival analysis suggested a median duration of 58 days (interquartile range, 40-110 days). | 2014 | 26625456 |
| bordetella pertussis diagnosis in children under five years of age in the regional hospital of cajamarca, northern peru. | bordetella pertussis is an important human pathogen that causes whooping cough (pertussis), an endemic illness responsible of significant morbidity and mortality, especially in infants and children. worldwide, there are an estimated of 16 million cases of pertussis, resulting in about 195,000 child deaths per year. in peru, pertussis is a major health problem that has been on the increase despite immunization efforts. the objective of this study was to determine the prevalence of b. pertussis am ... | 2015 | 26623626 |
| eight-year review of bordetella pertussis testing reveals seasonal pattern in the united states. | review of bordetella pertussis polymerase chain reaction testing from 2007 through 2014 revealed a yearly spike in positivity rates during the summer throughout the united states. paradoxically, the highest test volumes occurred outside of this time frame, which provides an opportunity for improved test utilization. | 2015 | 26621328 |
| adenylate cyclase toxin-mediated delivery of the s1 subunit of pertussis toxin into mammalian cells. | the adenylate cyclase toxin (act) of bordetella pertussis internalizes its catalytic domain into target cells. act can function as a tool for delivering foreign protein antigen moieties into immune effector cells to induce a cytotoxic t lymphocyte response. in this study, we replaced the catalytic domain of act with an enzymatically active protein moiety, the s1 (adp-ribosyltransferase) subunit of pertussis toxin (pt). the s1 moiety was successfully internalized independent of endocytosis into s ... | 2016 | 26607401 |
| temperature accelerated molecular dynamics with soft-ratcheting criterion orients enhanced sampling by low-resolution information. | many proteins exhibit an equilibrium between multiple conformations, some of them being characterized only by low-resolution information. visiting all conformations is a demanding task for computational techniques performing enhanced but unfocused exploration of collective variable (cv) space. otherwise, pulling a structure toward a target condition biases the exploration in a way difficult to assess. to address this problem, we introduce here the soft-ratcheting temperature-accelerated molecula ... | 2015 | 26575778 |
| validation of a loop-mediated isothermal amplification assay for rapid diagnosis of pertussis infection in nasopharyngeal samples. | to develop and validate a novel loop-mediated amplification (lamp) assay for rapid diagnosis (<1 hour) of whooping cough in nasopharyngeal samples versus the gold standard: real-time pcr. | 2016 | 26565672 |
| pertussis vaccines: who position paper, august 2015--recommendations. | this article presents the world health organization's (who) recommendations for the use of vaccines against bordetella pertussis from the who position paper on pertussis vaccines: who position paper--august 2015, recently published in the weekly epidemiological record (pertussis vaccines: who position paper. wkly epidemiol rec 2015;90(august(35)):433-60). this position paper summarizes the most recent developments in the field of pertussis disease and its prevention by vaccination. it includes t ... | 2016 | 26562318 |
| absence of bordetella pertussis among infants hospitalized for bronchiolitis in finland, 2008-2010. | in 169 finnish infants hospitalized for bronchiolitis at age <6 months in 2008-2010, nasopharyngeal aspirates were tested by polymerase chain reaction for bordetella pertussis and 16 viruses. respiratory viruses were detected in 89% (71% with respiratory syncytial virus), but no infant had b. pertussis. the latter finding may reflect a positive effect from the broadening of the finnish pertussis vaccination program in 2005. | 2016 | 26551447 |
| protecting newborns against pertussis: treatment and prevention strategies. | pertussis is a potentially severe respiratory disease, which affects all age groups from young infants to older adults and is responsible for an estimated 195,000 deaths occurred globally in 2008. active research is ongoing to better understand the pathogenesis, immunology, and diagnosis of pertussis. for diagnosis, molecular assays (e.g., polymerase chain reaction) for detection of bordetella pertussis have become more widely available and support improved outbreak detection. in children, pertu ... | 2015 | 26542059 |
| detection of bordetella pertussis using a pcr test in infants younger than one year old hospitalized with whooping cough in five peruvian hospitals. | to report the incidence, epidemiology, and clinical features of bordetella pertussis in peruvian infants under 1 year old. | 2015 | 26523641 |
| community awareness and predictors of uptake of pertussis booster vaccine in south australian adults. | pertussis is a highly virulent vaccine preventable disease that remains a global challenge. this study aimed to assess community knowledge of pertussis infection as well as awareness and uptake of adult pertussis booster vaccine. | 2015 | 26514422 |
| highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis. | bordetella pertussis colonizes the human respiratory mucosa. most studies on b. pertussis adherence have relied on cultured mammalian cells that lack key features present in differentiated human airway cells or on animal models that are not natural hosts of b. pertussis. the objectives of this work were to evaluate b. pertussis infection in highly differentiated human airway cells in vitro and to show the role of b. pertussis fimbriae in cell adherence. | 2016 | 26492208 |
| plasmacytoid dendritic cell-derived ifnα modulates th17 differentiation during early bordetella pertussis infection in mice. | whooping cough is a highly contagious respiratory disease caused by bordetella pertussis (b. pertussis). t helper 17 (th17) cells have a central role in the resolution of the infection. emerging studies document that type i interferons (ifns) suppress th17 differentiation and interleukin (il)-17 responses in models of infection and chronic inflammation. as plasmacytoid dendritic cells (pdcs) are a major source of type i ifns, we hypothesize that during b. pertussis infection in mice, pdc-derived ... | 2016 | 26462419 |
| [antigenic variability of bordetella pertussis strains isolated in 1967-2010 in the czech republic--possible explanation for the rise in cases of pertussis?]. | comparison of antigenic structures of bordetella pertussis (b. pertussis) strains isolated from 1967 to 2010 in the czech republic. | 2015 | 26448300 |
| restricted antibody response to bordetella pertussis filamentous hemagglutinin induced by whole-cell and acellular pertussis vaccines. | filamentous hemagglutinin (fha) is a principal virulence factor, an important immunogenic antigen of bordetella pertussis, and a major component of many acellular pertussis vaccines. in the present study, the human antibody response to different regions of fha was determined in healthy children and adults vaccinated with either whole-cell or acellular pertussis vaccines. | 2016 | 26439274 |
| pore-formation by adenylate cyclase toxoid activates dendritic cells to prime cd8+ and cd4+ t cells. | the adenylate cyclase toxin-hemolysin (cyaa) of bordetella pertussis is a bi-functional leukotoxin. it penetrates myeloid phagocytes expressing the complement receptor 3 and delivers into their cytosol its n-terminal adenylate cyclase enzyme domain (~400 residues). in parallel, ~1300 residue-long rtx hemolysin moiety of cyaa forms cation-selective pores and permeabilizes target cell membrane for efflux of cytosolic potassium ions. the non-enzymatic cyaa-ac(-) toxoid, has repeatedly been successf ... | 2016 | 26437769 |
| pertactin negative bordetella pertussis demonstrates higher fitness under vaccine selection pressure in a mixed infection model. | whooping cough or pertussis is a highly infectious respiratory disease in humans caused by bordetella pertussis. the use of acellular vaccines (acv) has been associated with the recent resurgence of pertussis in developed countries including australia despite high vaccination coverage where b. pertussis strains that do not express pertactin (prn), a key antigenic component of the acv, have emerged and become prevalent. in this study, we used an in vivo competition assay in mice immunised with ac ... | 2015 | 26432908 |
| an in vitro assay for substrate translocation by fhac in liposomes. | the two-partner secretion (tps) pathway is used by gram-negative bacteria to secrete a large family of virulence exoproteins. its name is derived from the fact that it involves two proteins, a secreted tpsa protein and a cognate tpsb transporter in the outer membrane. a typical tps system is represented by the filamentous hemagglutinin fhab (tpsa protein) and its transporter fhac (tpsb protein) of bordetella pertussis. results from mutational analysis and heterologous expression experiments sugg ... | 2015 | 26427679 |
| genetic diversity and population dynamics of bordetella pertussis in china between 1950-2007. | pertussis is an acute respiratory infectious disease caused by the bacterium bordetella pertussis. although pertussis vaccination was introduced in the 1960s, pertussis is still an endemic disease in china. to better understand the genetic diversity of the chinese b. pertussis population, we characterized 115 clinical isolates obtained in china during 1950-2007 using multilocus variable-number tandem repeat analysis (mlva). forty-six different b. pertussis mlva profiles (mts) were identified, of ... | 2015 | 26409140 |
| [bordetella pertussis lipooligosaccharide-derived neoglycoconjugates - new components of pertussis vaccine]. | pertussis is a contagious respiratory tract disease caused by the gram-negative bacterium bordetella pertussis. despite widespread vaccination, in recent years the pertussis incidence has increased. the whole-cell pertussis vaccine has been very effective but reactogenic. therefore the improved vaccines contain only a few isolated and inactivated antigens of b. pertussis. however, a waning of the acellular vaccine-induced immunity indicates that these vaccines lack some important protective b. p ... | 2015 | 26400888 |
| a newborn with pertussis accompanying nephrotic syndrome. | pertussis or whooping cough is a vaccine-preventable disease that still remains a serious infection in neonates and young infants. the disease is particularly severe in infants less than three months old, who are often infected by their parents. congenital nephrotic syndrome is a rare entity presenting within the first three months. it encompasses a heterogeneous group of entities with genetic, infectious and idiopathic etiologies. in this report we describe a newborn infant who presented with c ... | 2015 | 26388601 |
| pertussis in children in the istanbul faculty of medicine: results for four years. | we investigated the frequency of pertussis among children in the istanbul faculty of medicine hospital during a period of four years. clinical specimens were obtained from children who exhibited symptoms of whooping cough; a portion of the cases were confirmed microbiologically by pcr as pertussis. a total of 410 nasopharyngeal aspirates were taken for detection of bordetella pertussis/parapertussis. the age groups of the patients were 0-4 months (n=201), >4 -12 months (n=49), 1-4 years (n=79), ... | 2015 | 26388594 |
| latent class analysis of diagnostic tests for adenovirus, bordetella pertussis and influenza virus infections in german adults with longer lasting coughs. | laboratory tests in adult outpatients with longer lasting coughs to identify a potential causal pathogen are rarely performed, and there is no gold standard for these diagnostic tests. while the diagnostic validity of serological tests for pertussis is well established their potential contribution for diagnosing adenovirus and influenza virus a and b infections is unclear. a sentinel study into the population-based incidence of longer lasting coughs in adults was done in rostock (former east ger ... | 2016 | 26380914 |
| functional importance of the gly cluster in transmembrane helix 2 of the bordetella pertussis cyaa-hemolysin: implications for toxin oligomerization and pore formation. | adenylate cyclase-hemolysin (cyaa) is a major virulence factor of bordetella pertussis causing whooping cough in humans. we previously showed that two transmembrane helices (α2 and α3) in the hemolysin domain (cyaa-hly) are crucially involved in hemolytic activity. here, pcr-based substitutions were employed to investigate a potential involvement in hemolysis of a series of four gly residues (gly(530), gly(533), gly(537) and gly(544)) which map onto one face of a helical wheel plot of pore-linin ... | 2015 | 26363293 |
| pertussis in the era of new strains of bordetella pertussis. | despite implementation of a successful vaccination program, pertussis remains a significant health problem. although the incidence of pertussis in the united states is reduced by approximately 80% compared with incidence before the introduction of vaccination in the 1940s, deaths still occur and the unrecognized disease burden remains high, with 1 million bordetella pertussis infections annually in the united states estimated by serologic surveys. reasons for the resurgence and current prevalenc ... | 2015 | 26337739 |
| camp signalling of bordetella adenylate cyclase toxin through the shp-1 phosphatase activates the bimel-bax pro-apoptotic cascade in phagocytes. | the adenylate cyclase toxin-hemolysin (cyaa, act or ac-hly) plays a key role in virulence of bordetella pertussis. cyaa penetrates myeloid cells expressing the complement receptor 3 (αm β2 integrin cd11b/cd18) and subverts bactericidal capacities of neutrophils and macrophages by catalysing unregulated conversion of cytosolic atp to the key signalling molecule adenosine 3',5'-cyclic monophosphate (camp). we show that the signalling of cyaa-produced camp hijacks, by an as yet unknown mechanism, t ... | 2016 | 26334669 |
| an ecological analysis of pertussis disease in minnesota, 2009-2013. | the increase in pertussis cases in minnesota in the last decade has been mainly attributed to the switch from whole cell to acellular pertussis [as part of the diphtheria, tetanus and acellular pertussis vaccine (dtap)]. it is unclear, however, to what degree community-level risk factors also contribute. understanding these factors can help inform public health policy-makers about where else to target resources. we performed an ecological analysis within minnesota to identify risk factors at the ... | 2016 | 26330135 |
| isolated cyaa-rtx subdomain from bordetella pertussis: structural and functional implications for its interaction with target erythrocyte membranes. | the 126-kda bordetella pertussis cyaa-hemolysin (cyaa-hly) was previously expressed in escherichia coli as a soluble precursor that can be acylated to retain hemolytic activity. here, we investigated structural and functional characteristics of a ∼100-kda isolated rtx (repeat-in-toxin) subdomain (cyaa-rtx) of cyaa-hly. initially, we succeeded in producing a large amount with high purity of the his-tagged cyaa-rtx fragment and in establishing the interaction of acylated cyaa-hly with sheep red bl ... | 2015 | 26325465 |
| the impact of parental postpartum pertussis vaccination on infection in infants: a population-based study of cocooning in western australia. | during a pertussis epidemic in 2011-2012 the western australian (wa) department of health implemented a 'cocooning' programme, offering free pertussis-containing vaccine (dtpa) to new parents. we assessed the impact of vaccinating parents with dtpa on the incidence of pertussis infection in newborns. births in wa during 2011-2012 were linked to a register of parental pertussis vaccinations and to notified reports of laboratory-proven pertussis in children <6 months of age. parents who received d ... | 2015 | 26320420 |