| use of ice-nucleating proteins to improve the performance of freeze-thaw valves in microfluidic devices. | currently, reliable valving on integrated microfluidic devices fabricated from rigid materials is confined to expensive and complex methods. freeze-thaw valves (ftvs) can provide a low cost, low complexity valving mechanism, but reliable implementation of them has been greatly hindered by the lack of ice nucleation sites within the valve body's small volume. work to date has required very low temperatures (on the order of -40 °c or colder) to induce freezing without nucleation sites, making ftvs ... | 2017 | 28467855 |
| selection and assessment of plant growth-promoting rhizobacteria for biological control of multiple plant diseases. | a study was designed to screen individual strains of plant growth-promoting rhizobacteria (pgpr) for broad-spectrum disease suppression in vitro and in planta. in a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. in a secondary screen, these 28 strains showed broad spectrum antagonistic activity to six different genera of pathogens, and 24 of the 28 strains produced five traits reported to be related to plant growth promotion, including nitrogen fixatio ... | 2017 | 28440700 |
| evading plant immunity: feedback control of the t3ss in pseudomonas syringae. | microbes are responsible for over 10% of the global yield losses in staple crops such as wheat, rice, and maize. understanding the decision-making strategies that enable bacterial plant pathogens to evade the host immune system and cause disease is essential for managing their ever growing threat to food security. many utilise the needle-like type iii secretion system (t3ss) to suppress plant immunity, by injecting effector proteins that inhibit eukaryotic signalling pathways into the host cell ... | 2017 | 28435841 |
| structural and stereoelectronic insights into oxygenase-catalyzed formation of ethylene from 2-oxoglutarate. | ethylene is important in industry and biological signaling. in plants, ethylene is produced by oxidation of 1-aminocyclopropane-1-carboxylic acid, as catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase. bacteria catalyze ethylene production, but via the four-electron oxidation of 2-oxoglutarate to give ethylene in an arginine-dependent reaction. crystallographic and biochemical studies on the pseudomonas syringae ethylene-forming enzyme reveal a branched mechanism. in one branch, an appar ... | 2017 | 28420789 |
| a signature of tree health? shifts in the microbiome and the ecological drivers of horse chestnut bleeding canker disease. | host susceptibility to pathogens can be shaped by genetic, ecological, and evolutionary factors. the ability to predict the spread of disease therefore requires an integrated understanding of these factors, including effects of pests on pathogen growth and competition between pathogens and commensal microbiota for host resources. we examined interactions between the leaf-mining moth cameraria ohridella, the bacterial causal agent of bleeding canker disease pseudomonas syringae pv aesculi, and th ... | 2017 | 28418070 |
| lsu network hubs integrate abiotic and biotic stress responses via interaction with the superoxide dismutase fsd2. | in natural environments, plants often experience different stresses simultaneously, and adverse abiotic conditions can weaken the plant immune system. interactome mapping revealed that the low sulphur upregulated (lsu) proteins are hubs in an arabidopsis protein interaction network that are targeted by virulence effectors from evolutionarily diverse pathogens. here we show that lsu proteins are up-regulated in several abiotic and biotic stress conditions, such as nutrient depletion or salt stres ... | 2017 | 28207043 |
| cross-talk between jasmonate and salicylate plant defense pathways: effects on several plant parasites. | plants are often attacked by many herbivorous insects and pathogens at the same time. two important suites of responses to attack are mediated by plant hormones, jasmonate and salicylate, which independently provide resistance to herbivorous insects and pathogens, respectively. several lines of evidence suggest that there is negative cross-talk between the jasmonate and salicylate response pathways. this biochemical link between general plant defense strategies means that deploying defenses agai ... | 2002 | 28547690 |