| myocardial abscess, a rare form of cardiac aspergillosis. | | 2014 | 23791602 |
| long-term antifungal treatment improves health status in patients with chronic pulmonary aspergillosis: a longitudinal analysis. | chronic pulmonary aspergillosis (cpa) is an infectious disease that progressively destroys lung tissue. to date, no longitudinal data on the efficacy of antifungal treatment on health status in cpa patients exist. | 2013 | 23788240 |
| volume dependency for culture of fungi from respiratory secretions and increased sensitivity of aspergillus quantitative pcr. | diagnosis of aspergillosis is often difficult. we compared fungal yields from respiratory specimens using the health protection agency standard culture method (bsop57), a higher volume undiluted culture method mycology reference centre manchester (mrcm) and aspergillus quantitative real time polymerase chain reaction (qpcr). sputum, bronchial aspirate and bronchoalveolar lavage (bal) samples (total 23) were collected from aspergillosis patients. one fraction of all samples was cultured using the ... | 2014 | 23786547 |
| deletion of the sec4 homolog srga from aspergillus fumigatus is associated with an impaired stress response, attenuated virulence and phenotypic heterogeneity. | small gtpases of the rab family are master regulators of membrane trafficking, responsible for coordinating the sorting, packaging and delivery of membrane-bound vesicles to specific sites within eukaryotic cells. the contribution of these proteins to the biology of the human pathogenic fungus aspergillus fumigatus has not been explored. in this study, we characterized the srga gene, encoding a rab gtpase closely related to sec4. we found that a gfp-srga fusion protein accumulated preferentially ... | 2013 | 23785510 |
| kb425796-a, a novel antifungal antibiotic produced by paenibacillus sp. 530603. | the novel antifungal macrocyclic lipopeptidolactone, kb425796-a (1), was isolated from the fermentation broth of bacterial strain 530603, which was identified as a new paenibacillus species based on morphological and physiological characteristics, and 16s rrna sequences. kb425796-a (1) was isolated as white powder by solvent extraction, hp-20 and ods-b column chromatography, and lyophilization, and was determined to have the molecular formula c79h115n19o18. kb425796-a (1) showed antifungal activ ... | 2013 | 23778117 |
| identification of ten kb425796-a congeners from paenibacillus sp. 530603 using an antifungal assay against aspergillus fumigatus in combination with micafungin. | the discovery and characterization of natural congeners is one approach for understanding the relationship between chemical structure and biological function. we recently isolated the novel antifungal metabolite kb425796-a produced by the recently isolated bacterium paenibacillus sp. 530603. on the basis of morphological changes of aspergillus fumigatus induced by kb425796-a in combination with micafungin, we developed a highly sensitive screening method for the specific detection of kb425796-a ... | 2013 | 23778114 |
| early diagnosis of cerebral aspergillosis with various methods: a case report. | the clinical and laboratory diagnosis of cerebral aspergillosis (ca) is problematic and mortality is quite high, even in cases receiving appropriate treatment. therefore, an early and accurate diagnosis may prove to be life saving in patients with the diagnosis of ca. in this report, a case of ca which developed in a pediatric patient with acute lymphoblastic leukemia (all) is presented. upon development of neutropenia and focal seizures in the left arm during implementation of the all treatment ... | 2013 | 23774978 |
| extraction optimization and bioactivity of polysaccharides from aspergillus fumigatus af1. | aspergillus fumigatus was used to produce polysaccharide using sodium hydroxide pretreated rice straw as the sole carbon source by submerged fermentation. response surface methodology (rsm) was used to optimize the extraction of polysaccharide from the mycelia of a. fumigatus (paf). a three-level, three-factor box-behnken design (bbd) was applied for experimental design and analyzed the results to obtain the optimal extraction parameters. rsm analysis indicated good correspondence between experi ... | 2013 | 23768581 |
| chemical composition and antimicrobial activity of the essential oils from four ruta species growing in algeria. | antimicrobial properties of plants essential oils have been investigated in order to suggest them as potential tools to overcome the microbial drug resistance and the increasing incidence of food borne diseases problems. the aim of this research is to study the antibacterial and antifungal effects of four traditional plants essential oils, ruta angustifolia, ruta chalepensis, ruta graveolens and ruta tuberculata, against standard bacterial and fungal strains. the chemical compounds of the oils w ... | 2013 | 23768355 |
| preliminary laboratory report of fungal infections associated with contaminated methylprednisolone injections. | in september 2012, the centers for disease control and prevention (cdc) initiated an outbreak investigation of fungal infections linked to injection of contaminated methylprednisolone acetate (mpa). between 2 october 2012 and 14 february 2013, the cdc laboratory received 799 fungal isolates or human specimens, including cerebrospinal fluid (csf), synovial fluid, and abscess tissue, from 469 case patients in 19 states. a novel broad-range pcr assay and dna sequencing were used to evaluate these s ... | 2013 | 23761142 |
| extrinsic extracellular dna leads to biofilm formation and colocalizes with matrix polysaccharides in the human pathogenic fungus aspergillus fumigatus. | the environmentally acquired fungal pathogen aspergillus fumigatus causes a variety of severe diseases. furthermore, it is often found colonizing the respiratory tract of patients suffering from cystic fibrosis. conidia of this filamentous fungus adhere to substrate surfaces and germinate to form biofilms comprised of dense hyphal networks embedded in an adhesive extracellular matrix (ecm), built predominantly of polysaccharides. these fungal microconsortia are likely to be of clinical relevance ... | 2013 | 23760756 |
| subunit architecture of the golgi dsc e3 ligase required for sterol regulatory element-binding protein (srebp) cleavage in fission yeast. | the membrane-bound sterol regulatory element-binding protein (srebp) transcription factors regulate lipogenesis in mammalian cells and are activated through sequential cleavage by the golgi-localized site-1 and site-2 proteases. the mechanism of fission yeast srebp cleavage is less well defined and, in contrast, requires the golgi-localized dsc e3 ligase complex. the dsc e3 ligase consists of five integral membrane subunits, dsc1 through dsc5, and resembles membrane e3 ligases that function in e ... | 2013 | 23760507 |
| cytokine and transcription factor expression by aspergillus fumigatus-stimulated peripheral blood mononuclear cells in dogs with sino-nasal aspergillosis. | the causal agent of sino-nasal aspergillosis is usually aspergillus fumigatus, which is a saprophytic and ubiquitous fungus that causes a severe rhinosinusitis in apparent healthy dogs. affected dogs do not have systemic immuno-suppression. it has been shown previously that dogs affected by this disease have local over-expression of interleukin (il)-10 and th1 cytokines in nasal mucosal tissue. the aim of the present study was to assess the response of peripheral blood mononuclear cells (pbmc) f ... | 2013 | 23759303 |
| discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans. | dermatophytes belonging to the trichophyton and arthroderma genera cause skin infections in humans and animals. from genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in aspergillus fumigatus. using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. when integrated into the model aspergillus nidulans host, a str ... | 2013 | 23758576 |
| synergistic antifungal activity of kb425796-c in combination with micafungin against aspergillus fumigatus and its efficacy in murine infection models. | kb425796-c is a novel antifungal metabolite produced by the newly isolated bacterial strain paenibacillus sp. no. 530603. this compound is a 40-membered macrocyclic lipopeptidolactone consisting of 12 amino acids and a 3-hydroxy-15-methylpalmitoyl moiety. kb425796-c displayed antifungal activity against micafungin-resistant fungi and was fungicidal to trichosporon asahii in vitro. in a murine systemic infection model of t. asahii, kb425796-c showed excellent efficacy upon i.p. administration at ... | 2013 | 23756682 |
| genetic and structural validation of aspergillus fumigatus udp-n-acetylglucosamine pyrophosphorylase as an antifungal target. | the sugar nucleotide udp-n-acetylglucosamine (udp-glcnac) is an essential metabolite in both prokaryotes and eukaryotes. in fungi, it is the precursor for the synthesis of chitin, an essential component of the fungal cell wall. udp-n-acetylglucosamine pyrophosphorylase (uap) is the final enzyme in eukaryotic udp-glcnac biosynthesis, converting utp and n-acetylglucosamine-1-phosphate (glcnac-1p) to udp-glcnac. as such, this enzyme may provide an attractive target against pathogenic fungi. here, w ... | 2013 | 23750903 |
| allergic bronchopulmonary aspergillosis: a clinico-serological correlation with radiologic profile. | to study the different types of radiological presentations of abpa in a tertiary clinic in northern india and analyze them with respect to serological profile and clinical characteristics. | 2013 | 23745673 |
| synthesis, spectroscopic studies, molecular modeling and antimicrobial activity of binuclear co(ii) and cu(ii) complexes of 4,6-diacetylresorcinol. | reactions of 4,6-diacetylresorcinol with different cobalt(ii) and copper(ii) salts viz., oac(-), cl(-), no3(-) and so4(2-), yielded a new series of binuclear metal complexes. reactions of the ligand with these metal ions in the presence of a secondary ligand (l') [o,o-donor; acetylacetone, n,o-donor; 8-hydroxyquinoline or n,n-donor; 1,10-phenanthroline and n,n,n',n'-tetramethylethylenediamine] in 1:2:2 (l:m:l') molar ratio yielded mixed-ligand complexes with different molar ratios. the metal com ... | 2013 | 23743042 |
| cell wall perturbation sensitizes fungi to the antimalarial drug chloroquine. | chloroquine (cq) has been a mainstay of antimalarial drug treatment for several decades. additional therapeutic actions of cq have been described, including some reports of fungal inhibition. here we investigated the action of cq in fungi, including the yeast model saccharomyces cerevisiae. a genomewide yeast deletion strain collection was screened against cq, revealing that bck1δ and slt2δ mutants of the cell wall integrity pathway are cq hypersensitive. this phenotype was rescued with sorbitol ... | 2013 | 23733464 |
| quaternized n-substituted carboxymethyl chitosan derivatives as antimicrobial agents. | introduction of quaternary ammonium moieties into n-substituted carboxymethyl chitosan (n-substituted cmch) derivatives enhances their biological activity. several derivatives of cmch having a variety of n-aryl substituents bearing either electron-donating or electron withdrawing groups have been synthesized by the reaction between amino group of cmch with various aromatic aldehydes under acidic conditions, followed by reduction of the produced schiff base derivatives with sodium cyanoborohydrid ... | 2013 | 23732327 |
| structural requirements for sterol regulatory element-binding protein (srebp) cleavage in fission yeast. | sterol regulatory element-binding proteins (srebps) are central regulators of cellular lipid synthesis and homeostasis. mammalian srebps are proteolytically activated and liberated from the membrane by golgi site-1 and site-2 proteases. fission yeast srebps, sre1 and sre2, employ a different mechanism that genetically requires the golgi dsc e3 ligase complex for cleavage activation. here, we established sre2 as a model to define structural requirements for srebp cleavage. we showed that sre2 cle ... | 2013 | 23729666 |
| novel immunologic classification of aspergillosis in adult cystic fibrosis. | patients with cystic fibrosis (cf) demonstrate a wide range of hypersensitivity responses to aspergillus, beyond allergic bronchopulmonary aspergillosis, which require classification. | 2013 | 23726262 |
| in vitro evaluation of portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. | propolis is a beehive product with a very complex chemical composition, used since ancient times in several therapeutic treatments. as a contribution to the improvement of drugs against several tropical diseases caused by protozoa, we screened portuguese propolis and its potential floral sources populus x canadensis and cistus ladanifer against plasmodium falciparum, leishmania infantum, trypanosoma brucei and trypanosoma cruzi. the toxicity against mrc-5 fibroblast cells was evaluated to assess ... | 2014 | 23722631 |
| characterization of a recombinant α-glucuronidase from aspergillus fumigatus. | the degradation of xylan requires the action of glycanases and esterases which hydrolyse, in a synergistic fashion, the main chain and the different substituents which decorate its structure. among the xylanolytic enzymes acting on side-chains are the α-glucuronidases (agua) (e.c. 3.2.1.139) which release methyl glucuronic acid residues. these are the least studies among the xylanolytic enzymes. in this work, the gene and cdna of an α-glucuronidase from a newly isolated strain of aspergillus fum ... | 2013 | 23719223 |
| multicenter study of isavuconazole mic distributions and epidemiological cutoff values for aspergillus spp. for the clsi m38-a2 broth microdilution method. | epidemiological cutoff values (ecvs) were established for the new triazole isavuconazole and aspergillus species wild-type (wt) mic distributions (organisms in a species-drug combination with no detectable acquired resistance mechanisms) that were defined with 855 aspergillus fumigatus, 444 a. flavus, 106 a. nidulans, 207 a. niger, 384 a. terreus, and 75 a. versicolor species complex isolates; 22 aspergillus section usti isolates were also included. clsi broth microdilution mic data gathered in ... | 2013 | 23716059 |
| single-dose pharmacodynamics of amphotericin b against aspergillus species in an in vitro pharmacokinetic/pharmacodynamic model. | conventional mic testing of amphotericin b results in narrow mic ranges challenging the detection of resistant strains. in order to discern amphotericin b pharmacodynamics, the in vitro activity of amphotericin b was studied against aspergillus isolates with the same mics by using a new in vitro pharmacokinetic/pharmacodynamic (pk/pd) model that simulates amphotericin b human plasma levels. clinical isolates of aspergillus fumigatus, a. terreus, and a. flavus with the same clinical and laborator ... | 2013 | 23716054 |
| stability of airborne microbes in the louvre museum over time. | the microbial content of air has as yet been little described, despite its public health implications, and there remains a lack of environmental microbial data on airborne microflora in enclosed spaces. in this context, the aim of this study was to characterize the diversity and dynamics of airborne microorganisms in the louvre museum using high-throughput molecular tools and to underline the microbial signature of indoor air in this human-occupied environment. this microbial community was monit ... | 2014 | 23710880 |
| image-based elisa on an activated polypropylene microtest plate--a spectrophotometer-free low cost assay technique. | in this communication, we report elisa technique on an activated polypropylene microtest plate (appµtp) as an illustrative example of a low cost diagnostic assay. activated test zone in appµtp binds a capture biomolecule through covalent linkage thereby, eliminating non-specific binding often prevalent in absorption based techniques. efficacy of appµtp is demonstrated by detecting human immunoglobulin g (igg), human immunoglobulin e (ige) and aspergillus fumigatus antibody in patient's sera. det ... | 2013 | 23708751 |
| molecular analysis of changes in pseudomonas aeruginosa load during treatment of a pulmonary exacerbation in cystic fibrosis. | intravenous antibiotics for pulmonary exacerbations (pes) of cystic fibrosis (cf) usually target pseudomonas aeruginosa. insights into the cf lung microbiome have questioned this approach. we used rt-qpcr to determine whether intravenous antibiotics reduced p. aeruginosa numbers and whether this correlated with improved lung function. we also investigated antibiotic effects on other common respiratory pathogens in cf. | 2013 | 23706827 |
| irf4 transcription factor-dependent cd11b+ dendritic cells in human and mouse control mucosal il-17 cytokine responses. | mouse and human dendritic cells (dcs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. here, we showed that murine lung and gut lamina propria cd11b+ dc populations were comprised of two subsets: flt3- and irf4-dependent cd24(+)cd64(-) dcs and contaminating csf-1r-dependent cd24(-)cd64(+) macrophages. functionally, loss of cd24(+)cd11b(+) dcs abrogated cd4+ t cell-mediated interleukin-17 (il-17) production in steady state and after a ... | 2013 | 23706669 |
| nitrogen regulation of virulence in clinically prevalent fungal pathogens. | the habitats of fungal pathogens range from environmental to commensal, and the nutrient content of these different niches varies considerably. upon infection of humans, nutrient availability changes significantly depending on the site and pathophysiology of infection. nonetheless, a common feature enabling successful establishment in these niches is the ability to metabolise available nutrients including sources of nitrogen, carbon and essential metals such as iron. in particular, nitrogen sour ... | 2013 | 23701678 |
| characterization of the t-cell-mediated immune response against the aspergillus fumigatus proteins crf1 and catalase 1 in healthy individuals. | invasive aspergillosis is a serious infectious complication after allogeneic stem cell transplantation. one of the strategies to improve the management of aspergillosis is the adoptive transfer of antigen-specific t cells, the success of which depends on the development of a broad repertoire of antigen-specific t cells. in this study, we identified cd4+ t cells specific for the aspergillus proteins crf1 and catalase 1 in 18 of 24 healthy donors by intracellular staining for interferon γ and cd15 ... | 2013 | 23698813 |
| azole-resistant aspergillus fumigatus, iran. | | 2013 | 23697829 |
| molecular characterization of afuflea, an l-fucose-specific lectin from aspergillus fumigatus. | aspergillus fumigatus causes life-threatening infections in immunocompromised patients. we have found that extracts of mycelial mats of a. fumigatus contain a potent hemagglutinin. to clarify the characteristics of this factor, the hemagglutinin was purified from late-stage cultures and characterized at the molecular level. the hemagglutinin is a 32-kilodalton protein that shows activity as an l-fucose lectin. the gene encoding this protein, afuflea, was identified from a genomic dna library uti ... | 2013 | 23695231 |
| comparative proteomic analyses reveal that flba down-regulates glit expression and sod activity in aspergillus fumigatus. | flba is a regulator of g-protein signaling protein that plays a central role in attenuating heterotrimeric g-protein mediated vegetative growth signaling in aspergillus. the deletion of flba (∆flba) in the opportunistic human pathogen aspergillus fumigatus results in accelerated cell death and autolysis in submerged culture. to further investigate the effects of ∆flba on intracellular protein levels we carried out 2-d proteome analyses of 2-day old submerged cultures of ∆flba and wild type (wt) ... | 2013 | 23689084 |
| incidence of pulmonary aspergillosis and correlation of conventional diagnostic methods with nested pcr and real-time pcr assay using bal fluid in intensive care unit patients. | although the incidence of invasive aspergillosis in the intensive care unit (icu) is scarce, it has emerged as major problems in critically ill patients. in this study, the incidence of pulmonary aspergillosis (pa) in icu patients has evaluated and direct microscopy and culture has compared with nested polymerase chain reaction (pcr) and real-time pcr for detection of aspergillus fumigatus and a. flavus in bronchoalveolar lavage (bal) samples of the patients. | 2013 | 23686776 |
| anthraquinone derivatives from rumex plants and endophytic aspergillus fumigatus and their effects on diabetic nephropathy. | two new oxanthrone c-glycosides, patientosides a (14) and b (15), together with three known ones (11-13), were isolated from rumex patientia. their structures were identified on the basis of spectroscopic methods. the absolute configuration for 14 and 15 were deduced by analysis of their cd spectra and comparison with those of known similar compounds. compounds 11-15, and 14 known anthraquinones (1-4, 6-10, 16-20) previously isolated from rumex nepalensis, rumex hastatus, and endophytic aspergil ... | 2013 | 23683594 |
| [primary cutaneous aspergillosis in an extremely low birth weight preterm infant]. | a small hyperpigmented nodule 4 mm in diameter with a smaller satellite lesion was noted on the left hip 5 weeks after spontaneous birth of an otherwise unharmed 490 g female infant at 23 + 5 weeks of gestation. the mother had been treated with antibiotics for a clinically suspected amniotic infection syndrome. aspergillus fumigatus was identified in both repeated swabs of the lesions and culture of the resected tissue. the infant received liposomal amphotericin b (3 mg/kg/day) for 8 days. no ne ... | 2013 | 23681428 |
| spinal and paraspinal infections associated with contaminated methylprednisolone acetate injections - michigan, 2012-2013. | as of may 6, 2013, michigan had reported 167 (52%) of the 320 paraspinal or spinal infections without meningitis associated with the 2012-2013 fungal meningitis outbreak nationally. although the index patient had a laboratory-confirmed aspergillus fumigatus infection, the fungus most often identified, including in unopened vials of methylprednisolone acetate (mpa), remains exserohilum rostratum, a common black mold found on plants and in soil. exposures have occurred through epidural, paraspinal ... | 2013 | 23677044 |
| the developmental regulators, flbb and flbe, are involved in the virulence of aspergillus fumigatus. | several upstream activators required for proper activation of brla are involved in the development, vegetative growth, toxin production, and pathogenesis of aspergillus fumigatus. in this study, we characterized the roles of two upstream developmental regulators, a. fumigatus flbb (afuflbb) and flbe (afuflbe), in toxin production and virulence. the deletion of afuflbb and afuflbe resulted in reduction of the expression of afulaea. moreover, only about 8% to 10% of fumagillin was produced in the ... | 2013 | 23676908 |
| effects of chirality on the antifungal potency of methylated succinimides obtained by aspergillus fumigatus biotransformations. comparison with racemic ones. | eighteen (3r) and (3r,4r)-n-phenyl-, n-phenylalkyl and n-arylsuccinimides were prepared with high enantioselectivity by biotransformation of maleimides with a. fumigatus. this environmentally friendly, clean and economical procedure was performed by the whole-cell fungal bioconversion methodology. their corresponding eighteen racemic succinimides were prepared instead by synthetic methods. both, the racemic and the chiral succinimides were tested simultaneously by the microbroth dilution method ... | 2013 | 23676473 |
| rsma regulates aspergillus fumigatus gliotoxin cluster metabolites including cyclo(l-phe-l-ser), a potential new diagnostic marker for invasive aspergillosis. | dimeric basic leucine zipper (bzip) proteins are conserved transcriptional enhancers found in all eukaryotes. a recently reported and novel function for bzips is association of these proteins with secondary metabolite production in filamentous fungi. in particular a yap-like bzip termed rsma (restorer of secondary metabolism a) was identified in aspergillus nidulans that positively regulates the carcinogen sterigmatocystin. to assess for conserved function for rsma, we examined a role of this pr ... | 2013 | 23671611 |
| cyp51a-based mechanisms of aspergillus fumigatus azole drug resistance present in clinical samples from germany. | since the mid-1990s, a steady increase in the occurrence of itraconazole-resistant aspergillus fumigatus isolates has been observed in clinical contexts, leading to therapeutic failure in the treatment of aspergillosis. this increase has been predominantly linked to a single allele of the cyp51a gene, termed tr/l98h, which is thought to have arisen through the use of agricultural azoles. here, we investigated the current epidemiology of triazole-resistant a. fumigatus and underlying cyp51a mutat ... | 2013 | 23669382 |
| population-based survey of filamentous fungi and antifungal resistance in spain (filpop study). | a population-based survey was conducted to investigate the epidemiology of and antifungal resistance in spanish clinical strains of filamentous fungi isolated from deep tissue samples, blood cultures, and respiratory samples. the study was conducted in two different periods (october 2010 and may 2011) to analyze seasonal variations. a total of 325 strains were isolated in 29 different hospitals. the average prevalence was 0.016/1,000 inhabitants [corrected]. strains were identified by sequencing ... | 2013 | 23669377 |
| environmental study of azole-resistant aspergillus fumigatus with tr34/l98h mutations in the cyp51a gene in iran. | azole resistance in aspergillus is emerging in european and asian countries. as azoles are mainstay of therapy in the management of aspergillosis, azole resistance has serious implications in patient management. we report the emergence of resistance to triazoles in environmental aspergillus fumigatus isolates in iran. the tr34/l98h mutation was the only resistance mechanism. overall 3.3% of the a. fumigatus isolates from hospital surroundings in sari and tehran had the same tr34/l98h straf genot ... | 2013 | 23668594 |
| voriconazole resistance in aspergillus fumigatus: should we be concerned? | | 2013 | 23667265 |
| aspergillosis due to voriconazole highly resistant aspergillus fumigatus and recovery of genetically related resistant isolates from domiciles. | azole resistance is an emerging problem in aspergillus fumigatus and complicates the management of patients with aspergillus-related diseases. selection of azole resistance may occur through exposure to azole fungicides in the environment. in the netherlands a surveillance network was used to investigate the epidemiology of resistance selection in a. fumigatus. | 2013 | 23667263 |
| crude cellulase from oil palm empty fruit bunch by trichoderma asperellum upm1 and aspergillus fumigatus upm2 for fermentable sugars production. | cellulase is an enzyme that converts the polymer structure of polysaccharides into fermentable sugars. the high market demand for this enzyme together with the variety of applications in the industry has brought the research on cellulase into focus. in this study, crude cellulase was produced from oil palm empty fruit bunch (opefb) pretreated with 2% naoh with autoclave, which was composed of 59.7% cellulose, 21.6% hemicellulose, and 12.3% lignin using trichoderma asperellum upm1 and aspergillus ... | 2013 | 23666614 |
| evaluation of pharmacological activities, cytotoxicity and phenolic composition of four maytenus species used in southern african traditional medicine to treat intestinal infections and diarrhoeal diseases. | microbial infections and resulting inflammation and oxidative stress are common pathogenesis of gastrointestinal tract (git) disorders. in south africa, several species of the genus maytenus are used in traditional medicine to treat various infectious diseases. most of the previous work on this genus was focused on nonpolar extracts from the root and bark. in this study, leaf extracts of polar extracts of maytenus peduncularis, maytenus procumbens, maytenus senegalensis and maytenus undata were ... | 2013 | 23663902 |
| cardiac device-related invasive aspergilloma assessed by live/real time three-dimensional transthoracic echocardiography. | cardiac device-related infection caused by aspergillus species is a rare finding associated with high mortality. prompt recognition and treatment is imperative, but difficult as blood cultures are often negative and diagnosis requires a high index of suspicion. live/real time three-dimensional transthoracic echocardiography (3dtte) provides incremental knowledge in the characterization of valvular vegetations. here, we provide a detailed description of an invasive cardiac device-related infectio ... | 2013 | 23662919 |
| [preliminary study on aspergillus fumigatus-induced ikkα controlled maspin protein expression in respiratory epithelial cells of rats]. | to study if the expression of maspin protein in respiratory epithelial cells was downregulated through iκb kinase-α (ikkα)-controlled mechanism in an aspergillus fumigatus-induced model in rat. | 2013 | 23656816 |
| invasive fungal infections in patients with chronic granulomatous disease. | invasive fungal infections are a major threat for chronic granulomatous disease (cgd) patients. the present study provides a comprehensive overview of published invasive fungal infections in the cgd host through an extensive review of epidemiological, clinical, diagnostic and therapeutic data. in addition to the often mild clinical presentation, the currently used diagnostics for invasive aspergillosis have low sensitivity in cgd patients and cannot be easily translated to this non-neutropenic h ... | 2013 | 23654055 |
| modified culture method detects a high diversity of fungal species in cystic fibrosis patients. | cystic fibrosis (cf) is one of the most common genetic lung diseases worldwide. the production of sticky viscous mucus leads to enhanced bacterial colonization and infection, but yeasts and filamentous fungi are also found abundantly in the mucus of patients suffering from cf. the role of fungi in the airways of cf patients is still not understood completely. furthermore, recent investigations have shown that the spectrum of fungi isolated from the airways of cf patients depends strongly on the ... | 2014 | 23651180 |
| inflammatory marker profiles in an avian experimental model of aspergillosis. | aspergillosis is a common infection in avian species, but can be a challenge to diagnose. inflammatory markers have been successfully used in mammals for diagnostic and monitoring purposes of various diseases. the aim of this study was to identify inflammatory markers that could aid in the diagnosis of aspergillosis in an avian species. five-week-old japanese quail were infected experimentally with aspergillus fumigatus, and inflammatory markers were measured in plasma. in addition, lung tissues ... | 2013 | 23651177 |
| n-glycosylation of gel1 or gel2 is vital for cell wall β-glucan synthesis in aspergillus fumigatus. | fungal cell wall is a dynamic structure that communicates with and protects the cell from outside stress. in aspergillus fumigatus, the cell wall β-glucans are mainly elongated by β-1,3-glucanosyltransferases gels, which consist of seven family members (gel1-7) utilizing β-1,3-glucan chains as substrates. previously, we have shown that the mutant deficient of n-glycan processing displays a reduction in the cell wall β-glucans, suggesting that n-glycosylation is required for the proper function o ... | 2013 | 23650256 |
| bacterium induces cryptic meroterpenoid pathway in the pathogenic fungus aspergillus fumigatus. | stimulating encounter: the intimate, physical interaction between the soil-derived bacterium streptomyces rapamycinicus and the human pathogenic fungus aspergillus fumigatus led to the activation of an otherwise silent polyketide synthase (pks) gene cluster coding for an unusual prenylated polyphenol (fumicycline a). the meroterpenoid pathway is regulated by a pathway-specific activator gene as well as by epigenetic factors. | 2013 | 23649940 |
| a point mutation in ftmd blocks the fumitremorgin biosynthetic pathway in aspergillus fumigatus strain af293. | fumitremorgins (ftms), tremorgenic mycotoxins produced by the human pathogen aspergillus fumigatus, are prenylated indole alkaloids that have been extensively studied in view of their diverse chemical structures and biological activities. their biosynthetic gene (ftm) cluster was identified on the basis of the genome sequence of a. fumigatus. however, it has been reported that the ftm cluster in genome reference strain af293 is inactive, which makes complete understanding of the ftm pathway diff ... | 2013 | 23649274 |
| proteomic analysis of temperature dependent extracellular proteins from aspergillus fumigatus grown under solid-state culture condition. | fungal species of the genus aspergillus are filamentous ubiquitous saprophytes that play a major role in lignocellulosic biomass recycling and also are considered as cell factories for the production of organic acids, pharmaceuticals, and industrially important enzymes. analysis of extracellular secreted biomass degrading enzymes using complex lignocellulosic biomass as a substrate by solid-state fermentation could be a more practical approach to evaluate application of the enzymes for lignocell ... | 2013 | 23647126 |
| aspergillus fumigatus-induced il-22 is not restricted to a specific th cell subset and is dependent on complement receptor 3. | th cell responses induced by aspergillus fumigatus have been extensively investigated in mouse models. however, the requirements for differentiation and the characteristics of a. fumigatus-induced human th cell subsets remain poorly defined. we demonstrate that a. fumigatus induces th1 and th17 subsets in human pbmcs. moreover, we show that the cytokine il-22 is not restricted to a specific th subset, in contrast to il-17a. the pattern recognition and cytokine pathways that skew these aspergillu ... | 2013 | 23645883 |
| synthesis and antifungal activity of the novel triazole derivatives containing 1,2,3-triazole fragment. | a series of fluconazole analogues containing 1,2,3-triazole fragment have been designed and synthesized on the basis of the active site of the cytochrome p450 14α-demethylase (cyp51). their structures were characterized by (1)h nmr, (13)c nmr and lc-ms. the mic80 values indicate that the target compounds 1a-r showed higher activities against nearly all the fungi tested to some extent except against aspergillus fumigatus. compounds 1c, e, f, l and p showed 128 times higher activity (with the mic8 ... | 2013 | 23640383 |
| loss of ccla, required for histone 3 lysine 4 methylation, decreases growth but increases secondary metabolite production in aspergillus fumigatus. | secondary metabolite (sm) production in filamentous fungi is mechanistically associated with chromatin remodeling of specific sm clusters. one locus recently shown to be involved in sm suppression in aspergillus nidulans was ccla, a member of the histone 3 lysine 4 methylating compass complex. here we examine loss of ccla and a putative h3k4 demethylase, hdma, in the human pathogen aspergillus fumigatus. although deletion of hdma showed no phenotype under the conditions tested, the ccla deletant ... | 2013 | 23638376 |
| pretreatment with tlr2 and tlr4 ligand modulates innate immunity in corneal fibroblasts challenged with aspergillus fumigatus. | to study the innate immunity in telomerase-immortalized human stroma fibroblasts (thsfs) challenged with aspergillus fumigatus hyphae after copretreatment with tlr2 and tlr4 ligand. | 2013 | 23633661 |
| light sensing in aspergillus fumigatus highlights the case for establishing new models for fungal photobiology. | microbes inhabit diverse environmental locations, and many species need to shift their physiology between different niches. to do this effectively requires the accurate sensing of and response to the environment. for pathogens, exposure to light is one major change between a free-living saprophyte lifestyle and causation of disease within the host. however, how light may act as a signal to influence pathogenesis, on the side of either the host or the pathogen, is poorly understood. research duri ... | 2013 | 23631920 |
| is azole resistance in aspergillus fumigatus a problem in spain? | aspergillus fumigatus complex comprises a. fumigatus and other morphologically indistinguishable cryptic species. we retrospectively studied 362 a. fumigatus complex isolates (353 samples) from 150 patients with proven or probable invasive aspergillosis or aspergilloma (2, 121, and 6 samples, respectively) admitted to the hospital from 1999 to 2011. isolates were identified using the β-tubulin gene, and only 1 isolate per species found in each sample was selected. antifungal susceptibility to az ... | 2013 | 23629706 |
| antifungal indole and pyrrolidine-2,4-dione derivative peptidomimetic lead design based on in silico study of bioactive peptide families. | the rise of opportunistic fungal infections highlights the need for development of new antimicrobial agents. antimicrobial peptides (amps) and antifungal peptides (afps) are among the agents with minimal resistance being developed against them, therefore they can be used as structural templates for design of new antimicrobial agents. | 2013 | 23626876 |
| clinical and pathological findings of concurrent poxvirus lesions and aspergillosis infection in canaries. | to investigate clinical, pathological and mycological findings in canaries, in which pox lesions and aspergillus fumigatus (a. fumigatus) infection were observed simultaneously. | 2013 | 23620834 |
| fungal siderophore biosynthesis is partially localized in peroxisomes. | siderophores play a central role in iron metabolism and virulence of most fungi. both aspergillus fumigatus and aspergillus nidulans excrete the siderophore triacetylfusarinine c (tafc) for iron acquisition. in a. fumigatus, green fluorescence protein-tagging revealed peroxisomal localization of the tafc biosynthetic enzymes sidi (mevalonyl-coa ligase), sidh (mevalonyl-coa hydratase) and sidf (anhydromevalonyl-coa transferase), while elimination of the peroxisomal targeting signal (pts) impaired ... | 2013 | 23617799 |
| β-1,3-glucan modifying enzymes in aspergillus fumigatus. | in aspergillus fumigatus like in other filamentous ascomycetes, β-1,3-glucan constitutes a prominent cell wall component being responsible for rigidity of the cell wall structure. in filamentous fungi, softening of the cell wall is absolutely required during conidial germination and hyphal branching. because of the central structure of β-1,3-glucans, it is expected that β-1,3-glucanases play a major role in cell wall softening. based on in silico and experimental data, this review gives an overv ... | 2013 | 23616783 |
| environmental fungicides and triazole resistance in aspergillus. | fungal diseases are problematic in both human health and agriculture. treatment options are limited and resistance may emerge. the relatively recent recognition of triazole resistance in aspergillus fumigatus has prompted questioning of the origin of resistance. while multiple mechanisms are described in clinical isolates from triazole-treated patients, some de novo resistance is also recognised, especially attributable to tr34 /l98h. such strains probably arose in the environment, and, indeed, ... | 2014 | 23616354 |
| a 76-year-old man with a right lung adenocarcinoma and invasive aspergillosis. | a 76-year-old male with adenocarcinoma on the right lung underwent five cycles of chemotherapy with pemetrexed disodium, cisplatin, and dexamethasone. imaging studies of control showed a node in a cavitary lesion on the left lung, and the main hypothesis was aspergillus infection. pcr was utilized and contributed to establish the early diagnosis in this patient with invasive aspergillosis. furthermore, the species aspergillus fumigatus was characterized by its growing at 50 °c but not at 10 °c, ... | 2013 | 23615821 |
| production, purification, and characterization of exoglucanase by aspergillus fumigatus. | fungi are considered good producers of industrially valuable enzymes with higher enzymatic activities. among these cellulases are group of extracellular enzymes commonly employed in many industries for the hydrolysis of cellulolytic material. aspergillus fumigatus produced exoglucanase having high enzymatic activity (83 u/gds) during the solid-state fermentation of wheat straw under optimum physical and nutritional conditions. maximum production was obtained after 72 h of fermentation, at 55 °c ... | 2013 | 23615734 |
| [construction of a cell-surface expression system in trichoderma reesei]. | afmp1p is a glycosylphosphatidylinositol (gpi) anchored cell wall protein (gpi-cwp) identified in filamentous fungus aspergillus fumigatus, which contains a specific c terminal signal for cell wall localization. trichoderma reesei is known as a safe fungal species (gras) and widely used in the industry. thus, developing of the cell-surface expression systems in t. reesei is of industrial interest. | 2013 | 23614239 |
| multiple endobronchial mycetomas with varied appearances and mixed fungal flora. | we present images of a patient with multiple endobronchial mycetomas visible by bronchoscopy with varying appearances and with mixed fungal flora on culture. a 44-year-old african american male previously treated for pulmonary tuberculosis 11 years earlier presented with moderate hemoptysis and infiltrates in the right upper lobe on chest radiograph. he received levofloxacin without improvement in the infiltrate. computed tomography scan revealed bronchiectasis, multiple intrabronchial masses, a ... | 2013 | 23609249 |
| expression of the protein serum amyloid a in response to aspergillus fumigatus in murine models of allergic airway inflammation. | serum amyloid a (saa) is an acute phase protein that is elevated in blood during inflammation. the role of this protein in allergic diseases of airways remains unclear. | 2015 | 23603100 |
| secondary metabolites from the endophytic fungi penicillium polonicum and aspergillus fumigatus. | two new compounds, rhodostegone (1) from endophytic fungus penicillium polonicum and cyclo-(l-val-l-leu) (2) from aspergillus fumigatus, together with six known diketopiperazines (3-8), were isolated. the structures of these compounds were characterized through a combination of extensive ir, ms, nmr, and cd analysis. | 2013 | 23600807 |
| aspergillus fumigatus during stable state and exacerbations of copd. | bacteria are often isolated in stable chronic obstructive pulmonary disease (copd). whether fungi are also commonly present and associated with clinical and pathological features of disease is uncertain. we investigated the frequency of filamentous fungal culture and ige sensitisation to aspergillus fumigatus and the relationship to clinical outcomes in copd subjects. copd subjects were recruited to enter a 1-year observational study. assessments of lung function, allergen testing and sputum ana ... | 2014 | 23598955 |
| itraconazole as 'bridge therapy' to anti-ige in a patient with severe asthma with fungal sensitisation. | sensitisation to fungi has been reported to play an important role in a particular phenotype of severe asthma, the so-called severe asthma with fungal sensitisation, characterised by high levels of total ige, which may be an obstacle to anti-ige therapy. we describe here the case of a polysensitised woman with refractory asthma, sensitised to aspergillus fumigatus with high total ige values (1793 kua/l), but without the diagnostic criteria for allergic bronchopulmonary aspergillosis. additional ... | 2013 | 23598928 |
| low-volume toolbox for the discovery of immunosuppressive fungal secondary metabolites. | the secondary metabolome provides pathogenic fungi with a plethoric and versatile panel of molecules that can be deployed during host ingress. while powerful genetic and analytical chemistry methods have been developed to identify fungal secondary metabolites (sms), discovering the biological activity of sms remains an elusive yet critical task. here, we describe a process for identifying the immunosuppressive properties of aspergillus sms developed by coupling a cost-effective microfluidic neut ... | 2013 | 23592999 |
| assessment of efficacy of antifungals against aspergillus fumigatus: value of real-time bioluminescence imaging. | aspergillus fumigatus causes life-threatening infections, especially in immunocompromised patients. common drugs for therapy of aspergillosis are polyenes, azoles, and echinocandins. however, despite in vitro efficacy of these antifungals, treatment failure is frequently observed. in this study, we established bioluminescence imaging to monitor drug efficacy under in vitro and in vivo conditions. in vitro assays confirmed the effectiveness of liposomal amphotericin b, voriconazole, and anidulafu ... | 2013 | 23587947 |
| exposure assessment of lovastatin in pu-erh tea. | this paper reports the results of an extensive survey on the levels of lovastatin in pu-erh tea samples. the microbial source of lovastatin was assessed by testing the ability of fungi with higher isolation frequency in the pu-erh tea samples to produce lovastatin on czapek yeast extract agar (cya). lovastatin was not detected in any of the raw pu-erh tea samples without storage but was found in almost all the ripe pu-erh tea samples, with lovastatin contents ranging from 20.61 ng/gdw to 226.38 ... | 2013 | 23587710 |
| one single basic amino acid at the ω-1 or ω-2 site is a signal that retains glycosylphosphatidylinositol-anchored protein in the plasma membrane of aspergillus fumigatus. | although the plasma membrane is the terminal destination for glycosylphosphatidylinositol (gpi) proteins in higher eukaryotes, cell wall-attached gpi proteins (gpi-cwps) are found in many fungal species. in yeast, some of the cis-requirements directing localization of gpi proteins to the plasma membrane or cell wall are now understood. however, it remains to be determined how aspergillus fumigatus, an opportunistic fungal pathogen, signals, and sorts gpi proteins to either the plasma membrane or ... | 2013 | 23584992 |
| the cdr1b efflux transporter is associated with non-cyp51a-mediated itraconazole resistance in aspergillus fumigatus. | recent increases in triazole resistance in aspergillus fumigatus have been attributed primarily to target site (cyp51a) mutations. a recent survey of resistant isolates in manchester showed that >50% of resistant isolates had no mutation in cyp51a or its promoter. we investigated the mechanisms of resistance in clinical azole-resistant isolates without cyp51a mutations. | 2013 | 23580559 |
| clinical-scale generation of multi-specific anti-fungal t cells targeting candida, aspergillus and mucormycetes. | invasive fungal infections, in particular, infections caused by candida, aspergillus and mucormycetes, are a major cause of morbidity and mortality in patients undergoing allogeneic hematopoietic stem cell transplantation. adoptive transfer of donor-derived anti-fungal t cells shows promise to restore immunity and to offer a cure. because t cells recognize only specific epitopes, the low rate of patients in which the causal fungal pathogen can be identified and the considerable number of patient ... | 2013 | 23579059 |
| active site model of (r)-selective ω-transaminase and its application to the production of d-amino acids. | ω-transaminase (ω-ta) is one of the important biocatalytic toolkits owing to its unique enzyme property which enables the transfer of an amino group between primary amines and carbonyl compounds. in addition to preparation of chiral amines, ω-ta reactions have been exploited for the asymmetric synthesis of l-amino acids using (s)-selective ω-tas. however, despite the availability of (r)-selective ω-tas, catalytic utility of the ω-tas has not been explored for the production of d-amino acids. her ... | 2014 | 23576035 |
| volatile composition and antimicrobial activity of the essential oil of artemisia absinthium growing in western ghats region of north west karnataka, india. | artemisia absinthium l. (asteraceae) is an aromatic, herbaceous, perennial plant commonly known as wormwood. artemisia absinthium is traditionally used as an anthelmintic, antiseptic, antispasmodic and for bacillary dysentery, cancers and neurodegenerative diseases. | 2013 | 23570523 |
| changes in atmospheric co2 influence the allergenicity of aspergillus fumigatus. | increased susceptibility to allergies has been documented in the western world in recent decades. however, a comprehensive understanding of its causes is not yet available. it is therefore essential to understand trends and mechanisms of allergy-inducing agents, such as fungal conidia. in this study, we investigated the hypothesis that environmental conditions linked to global atmospheric changes can affect the allergenicity of aspergillus fumigatus, a common allergenic fungal species in indoor ... | 2013 | 23568517 |
| characterization of homocysteine γ-lyase from submerged and solid cultures of aspergillus fumigatus ash (jx006238). | among 25 isolates, aspergillus fumigatus ash (jx006238) was identified as a potent producer of homocysteine gamma- lyase. the nutritional requirements to maximize the enzyme yield were optimized under submerged (sf) and solid-state fermentation (ssf) conditions, resulting in a 5.2- and 2.3-fold increase, respectively, after the last purification step. the enzyme exhibited a single homogenous band of 50 kda on sds-page, along with an optimum ph of 7.8 and ph stability range of 6.5 to 7.8. it also ... | 2013 | 23568204 |
| design, synthesis and antimicrobial activity of novel benzothiazole analogs. | in an attempt to design and synthesize a new class of antimicrobials, dialkyne substituted 2-aminobenzothiazole was reacted with various substituted aryl azides to generate a small library of 20 compounds (3a-t) by click chemistry. structures of the newly synthesized compounds were established on the basis of spectral data. these compounds were screened for their antibacterial activity against gram+ bacteria (staphylococcus aureus and enterococcus faecalis), gram- bacteria (salmonella typhi, esc ... | 2013 | 23567952 |
| chlorine gas exposure increases susceptibility to invasive lung fungal infection. | chlorine (cl₂) is a highly irritating and reactive gas with potential occupational and environmental hazards. acute exposure to cl₂ induces severe epithelial damage, airway hyperreactivity, impaired alveolar fluid clearance, and pulmonary edema in the presence of heightened inflammation and significant neutrophil accumulation in the lungs. herein, we investigated whether cl₂ exposure affected the lung antimicrobial immune response leading to increased susceptibility to opportunistic infections. ... | 2013 | 23564508 |
| structure-activity relationship of cytochrome bc1 reductase inhibitor broad spectrum antifungal ilicicolin h. | ilicicolin h is a broad spectrum antifungal agent showing sub micro g/ml mics against candida spp., aspergillus fumigatus and cryptococcus spp. it is a potent inhibitor (c50 2-3ng/ml) of the mitochondrial cytochrome bc1 reductase with over 1000-fold selectivity against rat liver cytochrome bc1 reductase. structure-activity relationship of semisynthetic derivatives by chemical modification of ilicicolin h and its 19-hydroxy derivative produced by biotransformation have been described. basic 4'-es ... | 2013 | 23562597 |
| azole resistant aspergillus fumigatus: an emerging problem. | azole resistance has appeared recently in aspergillus fumigatus and increased dangerously in the last decade. the main resistance mechanism is a point mutation of cyp51a, the gene encoding 14α-sterol demethylase, the target enzyme of azole antifungal drugs. this mutation can induce resistance to itraconazole alone or multi-azole resistance. cyp51a mutation can occur in two cases. the first usually concerns patients receiving long-term azole therapy, most of the time for chronic aspergillosis, an ... | 2013 | 23562488 |
| deciphering the aetiology of a mixed fungal infection by broad-range pcr with sequencing and fluorescence in situ hybridisation. | simultaneous infections with multiple fungi may be misinterpreted as monomicrobial infections by current diagnostics with ramifications for the choice of antimicrobial agents that may impact patient outcomes. the application of molecular methods on tissue samples may be useful to decipher the aetiology of mixed fungal infections. we present a leukaemic patient who died from sepsis due to candidaemia. the postmortem examination documented fungal elements in lung tissue. fungal dna was amplified f ... | 2013 | 23560622 |
| reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in saccharomyces cerevisiae. | fungal polyketides include commercially important pharmaceuticals and food additives, e.g. the cholesterol-lowering statins and the red and orange monascus pigments. presently, production relies on isolation of the compounds from the natural producers, and systems for heterologous production in easily fermentable and genetically engineerable organisms, such as saccharomyces cerevisiae and escherichia coli are desirable. rubrofusarin is an orange polyketide pigment that is a common intermediate i ... | 2013 | 23557488 |
| biological activities of selected basidiomycetes from yemen. | in a previous paper we demonstrated the results of biological screening of yemeni basidiomycetes. the present study was aimed to investigate the antimicrobial and the antioxidant activity of further basidiomycetes collected in yemen. dichloromethane, methanol and aqueous extracts of the fruiting bodies of 25 species were screened in vitro for their antibacterial activities against three gram-positive bacteria (staphyloccocus aureus, bacillus subtilis, micrococcus flavus) and two gram-negative ba ... | 2013 | 23556343 |
| complement component 3c3 and c3a receptor are required in chitin-dependent allergic sensitization to aspergillus fumigatus but dispensable in chitin-induced innate allergic inflammation. | levels of the anaphylatoxin c3a are increased in patients with asthma compared with those in nonasthmatics and increase further still during asthma exacerbations. however, the role of c3a during sensitization to allergen is poorly understood. sensitization to fungal allergens, such as aspergillus fumigatus, is a strong risk factor for the development of asthma. exposure to chitin, a structural polysaccharide of the fungal cell wall, induces innate allergic inflammation and may promote sensitizat ... | 2013 | 23549917 |
| culture condition-dependent metabolite profiling of aspergillus fumigatus with antifungal activity. | three sections of aspergillus (five species, 21 strains) were classified according to culture medium-dependent and time-dependent secondary metabolite profile-based chemotaxonomy. secondary metabolites were analysed by liquid chromatography-electrospray ionisation tandem mass spectrometry (lc-esi-ms-ms) and multivariate statistical methods. from the aspergillus sections that were cultured on malt extract agar (mea) and czapek yeast extract agar (cya) for 7, 12, and 16 d, aspergillus sections fum ... | 2013 | 23537878 |
| the fungal pathogen aspergillus fumigatus regulates growth, metabolism, and stress resistance in response to light. | light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. though much research has focused on signaling pathways in aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking. in this report, we demonstrate that the fungus does indeed respond to both blue and red portions of the visible spectrum. included in the a. fumigatus light response is a reduction in conidial germination rates, inc ... | 2013 | 23532976 |
| [functional role of protein kinase d1 in aspergillus fumigatus-induced activation of nuclear factor-κb signal pathway and transcription]. | to explore the functional role of protein kinase d1 (pkd1) in the activation of nuclear factor-κb (nf-κb) signal pathway and nf-κb transcription mediated by aspergillus fumigatus. | 2013 | 23529230 |
| preemptive treatment with voriconazole in lung transplant recipients. | invasive fungal infection (ifi) is associated with high mortality in lung transplant (ltx) recipients. data for voriconazole use in preemptive treatment remain scant. | 2013 | 23527908 |
| transcriptome and biochemical analysis reveals that suppression of gpi-anchor synthesis leads to autophagy and possible necroptosis in aspergillus fumigatus. | previously, it has been shown that gpi proteins are required for cell wall synthesis and organization in aspergillus fumigatus, a human opportunistic pathogen causing life-threatening invasive aspergillosis (ia) in immunocompromised patients. blocking gpi anchor synthesis leads to severe phenotypes such as cell wall defects, increased cell death, and attenuated virulence. however, the mechanism by which these phenotypes are induced is unclear. to gain insight into global effects of gpi anchoring ... | 2013 | 23527074 |