| binding site of ribosomal proteins on prokaryotic 5s ribonucleic acids: a study with ribonucleases. | the binding sites of ribosomal proteins l18 and l25 on 5s rna from escherichia coli were probed with ribonucleases a, t1, and t2 and a double helix specific cobra venom endonuclease. the results for the protein-rna complexes, which were compared with those for the free rna [douthwaite, s., & garrett, r. a. (1981) biochemistry 20, 7301--7307], reveal an extensive interaction site for protein l18 and a more localized one for l25. generally comparable results, with a few important differences, were ... | 1982 | 6178424 |
| immunochemical accessibility of ribosomal protein s4 in the 30 s ribosome. the interaction of s4 with s5 and s12. | the reactivity of protein s4-specific antibody preparations with 30 s ribosomal subunits and intermediates of in vitro subunit reconstitution has been characterized using a quantitative antibody binding assay. anti-s4 antibody preparations did not react with native 30 s ribosomal subunits; however, they did react with various subunit assembly intermediates that lacked proteins s5 and s12. the inclusion of proteins s5 and s12 in reconstituted particles resulted in a large decrease in anti-s4 reac ... | 1983 | 6188845 |
| [isolation, purification and characteristics of alpha-amylase from bacillus stearothermophilus]. | | 1982 | 6189370 |
| bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase. | the gene for thermostable alpha-amylase from the thermophilic bacterium bacillus stearothermophilus has been cloned and expressed in escherichia coli. each alpha-amylase-producing colony contained at least a 9.7-kilobase-pair (kb) chimeric plasmid composed of the vector pbr322 and a common 5.4-kb hindiii fragment of dna. b. stearothermophilus contains four plasmids with sizes from 12 kb to over 108 kb. restriction endonuclease analysis of these naturally occurring plasmids showed they also conta ... | 1983 | 6193526 |
| higher order structure in the 3'-minor domain of small subunit ribosomal rnas from a gram negative bacterium, a gram positive bacterium and a eukaryote. | an experimental approach was used to determine and compare the highest order structure within the 150 to 200 nucleotides at the 3'-ends of the rnas from the small ribosomal subunits of escherichia coli, bacillus stearothermophilus and saccharomyces cerevisiae. chemical reagents were employed to establish the degree of stacking and/or accessibility of each adenosine, guanosine and cytidine. the double helices were probed with a cobra venom ribonuclease from naja naja oxiana, and the relatively un ... | 1983 | 6194304 |
| studies on the mode of action of a bacteriocin produced by bacillus stearothermophilus. | thermocin 10 was purified by elution from a carboxymethyl cellulose column. the eluted thermocin was homogeneous and yielded single bands with identical relative mobility when stained for protein or glycoprotein. thermocin 10 inhibited rna synthesis 3 min after its addition. cell motility, oxygen uptake, atp synthesis, and dna synthesis were inhibited 10 min after the addition of thermocin 10. total cell protein was reduced to one quarter of its normal content in cells treated with thermocin. | 1983 | 6200195 |
| reconstitution of 50 s ribosomal subunits from bacillus stearothermophilus with 5 s rna from spinach chloroplasts and low-mr rna from mitochondria of locusta migratoria and bovine liver. | reconstitution experiments with 50 s ribosomal subunits from bacillus stearothermophilus demonstrate that spinach chloroplast 5 s rrna can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5 s rrna. in contrast, mitochondria from locusta migratoria or bovine liver do not appear to contain discrete, low-mr rnas, which can replace 5 s rrna in the functional reconstitution of b. stearothermophilus ribosome ... | 1984 | 6201395 |
| comparative structural analysis of eubacterial 5s rrna by oxidation of adenines in the n-1 position. | adenines in free 5s rrna from escherichia coli, bacillus stearothermophilus and thermus thermophilus have been oxidized at their n-1 position using monoperphthalic acid. the determination of the number of adenine 1-n-oxides was on the basis of uv spectroscopic data of the intact molecule. identification of the most readily accessible nucleotides by sequencing gel analysis reveals that they are located in conserved positions within loops, exposed hairpin loops and single-base bulge loops. implica ... | 1984 | 6201825 |
| higher-order structure in the 3'-terminal domain vi of the 23 s ribosomal rnas from escherichia coli and bacillus stearothermophilus. | an experimental approach was used to determine, and compare, the higher-order structure within domain vi of the 23 s ribosomal rnas from escherichia coli and bacillus stearothermophilus. this domain, which encompasses approximately 300 nucleotides at the 3' end of the rnas, consists of two large subdomains. the 5' subdomain has been conserved during evolution and appears to be functionally important for the binding of the ef-1 x gtp x aminoacyl-trna complex in eukaryotes. the 3' subdomain has di ... | 1984 | 6209406 |
| specific ligand-induced association of an enzyme. a new model of dissociating allosteric enzyme. | the kinetic behavior of dissociative enzyme system of the type inactive monomer in equilibrium active dimer where dimeric form is stabilized by specific ligand (in particular by substrate) which is bound in the region of the contact of monomers has been analysed. it is assumed that the dissociation of dimer results in formation of monomers which retain the subsites for specific ligand binding. the shape of the dependences of enzyme reaction rate (v) on substrate concentration (s) has been charac ... | 1983 | 6225912 |
| [effect of mea on dna degradation in permeable irradiated bac. stearothermophilus cells]. | it was shown that dna-degrading activity of permeable, intact and gamma-irradiated cells of bac. stearothermophilus decreased under the effect of beta-mercaptoethylamine (mea). mea decreased also a dnaase activity, in a crude acellular extract of bac. stearothermophilus, and activities of s1-nuclease and dnaase i. the data obtained prompt an assumption that mea has an inhibitory action on the activity of endonucleases irrespective of their substrate specificity. | 1983 | 6228951 |
| evolution of phosphofructokinase--gene duplication and creation of new effector sites. | phosphofructokinases (pfk; ec 2.7.1.11) are tetrameric enzymes that have a key role in the regulation of glycolysis; as such, they are subject to allosteric activation and inhibition by various metabolites. eukaryotic pfks are about twice the size of prokaryotic enzymes and are regulated by a wider repertoire of effectors: for example, the subunit molecular weights of rabbit muscle (rm) pfk and bacillus stearothermophilus (bs) pfk are 82,000 and 36,000, respectively. both enzymes are activated b ... | 1984 | 6233492 |
| nucleotide sequence of gene pfkb encoding the minor phosphofructokinase of escherichia coli k-12. | the nucleotide sequence of a 1.3-kb dna fragment containing the entire pfkb gene which codes for pfk-2 of escherichia coli, a minor phosphofructokinase (pfk) enzyme, is reported. the pfk-2 protein subunit is encoded by 924 bp, has 308 amino acids and an mr of 33 000. like other weakly expressed e. coli genes the codon usage in the pfkb gene is random; there is no strong bias for the usage of major trna isoaccepting species, and the codon preference rules of grosjean and fiers [gene, 18 (1982) 19 ... | 1984 | 6235149 |
| conformational variability of nad+ in the free and bound states: a nicotinamide sandwich in nad+ crystals. | x-ray analysis of the free-acid crystal form of the coenzyme nicotinamide adenine dinucleotide (nad+) revealed a conformational difference between the free nad+ molecule and one bound in enzymes or complexed to li+ ions. the pyrophosphate group showed asymmetry in the phosphate-oxygen bonds of the phosphate-oxygen-phosphate link; this bond at the nicotinamide side of the link is longer than that at the adenosine side by 0.04 angstrom. the crystal structure showed a novel intermolecular stacking ... | 1984 | 6239374 |
| purification and properties of the bst1503 endonuclease. | | 1980 | 6246337 |
| proteolytic cleavage of methionyl transfer ribonucleic acid synthetase from bacillus stearothermophilus: effects on activity and structure. | methionyl-trna synthetase from bacillus stearothermophilus, a dimer of molecular weight 2 x 85k, is converted by limited subtilisin digestion into a fully active monomeric fragment of molecular weight 64k. the reversible methionine activation reaction of these enzymes was followed through the variation of the intensity of their trypotophan fluorescence. equilibrium and stopped-flow experiments show that the rate and mechanism for adenylate formation supported by the monomeric derivative are undi ... | 1980 | 6250575 |
| characterization of bacillus stearothermophilus plasmid pab124 and construction of deletion variants. | a restriction endonuclease cleavage map of the tetracycline resistance plasmid pab124, originally isolated from bacillus stearothermophilus, was constructed using ten enzymes. tetracycline resistance was associated with a 1 x 95 megadalton (md) region of pab124 lying between two ecori sites, and this region was circularized to produce a viable tetracycline resistance plasmid (pab224), with two ecori fragments of pab124 deleted amounting to 0 x 95 md. a second plasmid (pab524) with one ecori frag ... | 1980 | 6251158 |
| isozymes of alpha-galactosidase from bacillus stearothermophilus. | two molecular forms of alpha-galactosidase (ec 3.2.1.22) synthesized constitutively by bacillus stearothermophilus, strain at-7, have been purified. alpha-galactosidase i (with the substrate p-nitrophenyl alpha-d-galactopyranoside (pnpg)) has a ph optimum of 6 and half-life at 65 degrees c of > 2 h at low protein concentration. alpha-galactosidase ii has a ph optimum of 7 with pnpg and a half-life at 65 degrees c of about 3 min. the isozymes also differ with respect to their km with pnpg and mel ... | 1980 | 6257360 |
| isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. | ten plasmids were isolated as covalently closed circular deoxyribonucleic acid from antibiotic-resistant thermophilic bacteria. of the 10 plasmids tested, 2 could transform bacillus subtilis, yielding resistance to specific antibiotics. plasmid ptb20 (2.8 x 10(6) daltons, approximately 24 copies per chromosome) specifies resistance to tetracycline (tcr), whereas ptb19 (17.2 x 10(6) daltons, approximately 1 copy per chromosome) renders the host resistant to both kanamycin and tetracycline (kmrtcr ... | 1981 | 6263856 |
| the binding of nucleotides and metal ions to elongation factor tu from bacillus stearothermophilus as studied by equilibrium dialysis. | ef-tu from b. stearothermophilus binds divalent metal ions even in the absence of guanine nucleotides. the association constants necessary for characterizing the multiple equilibria between ef-tu, gdp and the divalent ions magnesium and manganese were determined by equilibrium dialysis. the constants are 4.6 x 10(4) m-1 and 5.4 x 10(5) m-1 for the binding of mg2 and 1.0 x 10(5) m-1 and 1.1 x 10(6) m-1 for the binding of mn2 to ef-tu and ef-tu . gdp, respectively. in the absence of divalent ions ... | 1981 | 6268521 |
| secondary structure of prokaryotic 5s ribosomal ribonucleic acids: a study with ribonucleases. | the structures of 5s ribosomal rnas from escherichia coli and bacillus stearothermophilus were examined by using ribonucleases a, t1, and t2 and a double helix specific cobra venom ribonuclease. by using both 5' and 3'-32p-end labeling methods and selecting for digested but intact 5s rna molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. the data reveal the predicted similarities of the higher order structu ... | 1981 | 6274397 |
| [microbial resistance to formaldehyde. ii. dependence of the microbicidal effect upon the concentration of and the period of exposure to formaldehyde (author's transl)]. | | 1981 | 6275632 |
| transformation of bacillus stearothermophilus with plasmid dna and characterization of shuttle vector plasmids between bacillus stearothermophilus and bacillus subtilis. | a thermophilic bacterium bacillus stearothermophilus ifo 12550 (atcc 12980) was transformed with each of the following plasmids, pub110 (kanamycin resistance, kmr), ptb19 (kmr and tetracycline resistance [tcr]), and its derivative ptb90 (kmr tcr), by the protoplast procedure in the presence of polyethylene glycol at 48 degrees c. the transformation frequencies per regenerant for pub110, ptb19, and ptb90 were 5.9 x 10(-3), 5.5 x 10(-3), and 2.0 x 10(-1), respectively. among these plasmids, ptb90 ... | 1982 | 6277855 |
| limited proteolysis and proton nmr spectroscopy of bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. | the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus was treated with chymotrypsin at ph 7 and 0 degrees c. loss of the overall catalytic activity lagged behind the rapid cleavage of the lipoate acetyltransferase polypeptide chains, whose apparent mr fell from 57 000 to 45 000 as judged by sodium dodecylsulphate/polyacrylamide gel electrophoresis. the inactive chymotrypsin-treated enzyme had lost the lipoic-acid-containing regions of the lipoate acetyltransferase chains, ... | 1982 | 6282590 |
| wet and dry bacterial spore densities determined by buoyant sedimentation. | the wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of high chemical density. with metrizamide or renografin, the wet density values for the spores and permeable sephadex beads were higher than those obtained by a reference direct mass method, and some spore populations were separated into several density bands. with percoll, all of the wet density value ... | 1982 | 6285824 |
| 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | | 1982 | 6292662 |
| acetate kinase from bacillus stearothermophilus. | | 1982 | 6296602 |
| on the dna binding protein ii from bacillus stearothermophilus. i. purification, studies in solution, and crystallization. | dna binding protein ii from bacillus stearothermophilus has been purified as a single species from the nonribosomal cell fraction by a combination of gel filtration and ion exchange chromatography. the protein occurs in solution as a tetramer and is able to bind to 30 s, 50 s, and 70 s ribosomal particles. circular dichroism studies show that the protein has approximately 45% alpha-helix. the secondary structure of the bacillus protein is considerably more resistant to the effects of increasing ... | 1983 | 6300068 |
| on the dna binding protein ii from bacillus stearothermophilus. ii. the amino acid sequence and its relation to those of homologous proteins from other prokaryotes. | the complete amino acid sequence of dna binding protein ii from bacillus stearothermophilus has been determined. the protein contains 90 amino acid residues and has a calculated mr of 9716. the sequence is compared to homologous molecules from escherichia coli, thermoplasma acidophilum, and pseudomonas aeruginosa (where only a partial sequence is available). the b. stearothermophilus molecule has 58% and 59% residues identical with the two forms of the e. coli protein and 32% with the t. acidoph ... | 1983 | 6300069 |
| molecular cloning of a thermostable neutral protease gene from bacillus stearothermophilus in a vector plasmid and its expression in bacillus stearothermophilus and bacillus subtilis. | the structural gene for a thermostable protease from bacillus stearothermophilus was cloned in plasmid ptb90. it is expressed in both b. stearothermophilus and bacillus subtilis. b. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 u/mg of cell dry weight) than did the wild-type strain of b. stearothermophilus. some properties of the proteases that have been purified from the transformants of b. stearothermophilus and b. subtilis were examined. no sign ... | 1983 | 6302083 |
| the application of triazine dye affinity chromatography to the large-scale purification of glycerokinase from bacillus stearothermophilus. | gram quantities of homogeneous glycerokinase have been prepared from the thermophilic bacterium, bacillus stearothermophilus, using three major steps: precipitation of debris at ph 5.1, ion-exchange chromatography on deae-sephadex, and affinity chromatography on procion blue mx-3g-sepharose. this method is a considerable improvement over conventional techniques; the purified enzyme was obtained with a 40% recovery and a specific activity of 120 units (mumol/min)/mg protein. a modified culture me ... | 1983 | 6312842 |
| deletion mutagenesis using an 'm13 splint': the n-terminal structural domain of tyrosyl-trna synthetase (b. stearothermophilus) catalyses the formation of tyrosyl adenylate. | the x-ray crystallographic structure of tyrosyl-trna synthetase (tyrts) comprises only the n-terminal 320 amino acids of the molecule as the c-terminal 99 amino acids are poorly ordered in the crystal. a new technique, employing a single-stranded m13 splint, has been used to direct a deletion in the cloned gene of tyrts so as to remove the disordered c-terminal region. we find that the truncated enzyme catalyses the formation of tyrosyl adenylate with unchanged kcat and km values and the crystal ... | 1983 | 6315404 |
| cloning and expression of a thermophilic alpha-amylase gene from bacillus stearothermophilus in escherichia coli. | a 6.4 kb hindiii fragment of bacillus stearothermophilus dy-5 dna cloned in escherichia coli using pbr322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. in attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 kb hindiii - bamhi fragment of the donor strain dna. the alpha-amylase gene was stably maintained and expressed efficiently in e. coli. the enzymic properties of alpha-amylase produced in e. coli closely resembl ... | 1984 | 6318051 |
| interaction of crystalline tyrosyl-trna synthetase with adenosine, adenosine monophosphate, adenosine triphosphate and pyrophosphate in the presence of tyrosinol. | crystalline complexes of tyrosyl-trna synthetase from bacillus stearothermophilus were prepared with adenosine, amp, atp and ppi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by atp. the hydrolysis of atp in the presence of crystalline tyrosyl-trna synthetase (or redissolved crystals) was checked in the absence of tyrosine or with tyrosinol. no atpase activity due to the enzyme was detected under these conditions. difference fourier ... | 1984 | 6323720 |
| q beta replicase containing a bacillus stearothermophilus elongation factor. | we purified q beta replicase containing ef-ts from bacillus stearothermophilus in place of the homologous polypeptide from escherichia coli. the hybrid enzyme was fully active in the transcription of a variety of templates. it was found to be qualitatively similar to native q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of rna synthesis. the results demonstrated that q beta replicase can tolerate substantial alterations in the ef-tu x ts compo ... | 1983 | 6337116 |
| automatic diluter for bacteriological samples. | the described apparatus, carrying 190 tubes, allows automatic and aseptic dilution of liquid or suspended-solid samples. serial 10-fold dilutions are programmable from 10(-1) to 10(-9) and are carried out in glass tubes with screw caps and split silicone septa. dilution assays performed with strains of escherichia coli and bacillus stearothermophilus permitted efficient conditions for sterilization of the needle to be defined and showed that the automatic dilutions were as accurate and as reprod ... | 1983 | 6338826 |
| chemical reactivity of e. coli 5s rna in situ in the 50s ribosomal subunit. | e. coli 50s ribosomal subunits were reacted with monoperphthalic acid under conditions in which non-base paired adenines are modified to their 1-n-oxides. 5s rna was isolated from such chemically reacted subunits and the two modified adenines were identified as a73 and a99. the modified 5s rna, when used in reconstitution of 50s subunits, yielded particles with reduced biological activity (50%). the results are discussed with respect to a recently proposed three-dimensional structure for 5s rna, ... | 1983 | 6340064 |
| mobility and active-site coupling in 2-oxo acid dehydrogenase complexes. | the 2-oxo acid dehydrogenase complexes consist of multiple copies of each of three enzymes, 2-oxo acid decarboxylase (e1), lipoate acetyltransferase (e2) and lipoamide dehydrogenase (e3), which catalyse successive steps in the overall reaction. the complexes are based on a structural core made up of the e2 chains, which also contain lipoic acid residues covalently attached to lysine residues. these lipoic acid residues are involved in transferring the substrate between the different active sites ... | 1983 | 6340997 |
| calorimetric measurements of the destabilisation of a ribosomal rna hairpin by dimethylation of two adjacent adenosines. | fragments of 16s ribosomal rna from e. coli and b. stearothermophilus, respectively comprising the 49 and 52 3' terminal nucleotides have been studied thermodynamically using high sensitivity differential scanning calorimetry. the fragments were isolated after cleavage of 16s rrna in the ribosome by the bacteriocin cloacin df13. comparison of the thermal properties of the e. coli fragments with those derived from a kasugamycin resistant mutant, which specifically lacks dimethylation of two adjac ... | 1983 | 6346264 |
| architecture of prokaryotic ribosomes. | | 1983 | 6351726 |
| temperature-dependence of intramolecular coupling of active sites in pyruvate dehydrogenase multienzyme complexes. | intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes of escherichia coli, ox heart and bacillus stearothermophilus was measured at various temperatures. as the temperature was raised, the extent of active-site coupling was found to increase, approaching a maximum near the physiological growth temperature of the organism. under these conditions, a single pyruvate dehydrogenase (lipoamide) dimer appeared able to cause a rapid (20s) reductive acetylation of pr ... | 1983 | 6351839 |
| a new fluorescent probe for the study of the allosteric properties of d-glyceraldehyde 3-phosphate dehydrogenase. | | 1983 | 6352362 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria. iii) the primary structure of thermophilic lactate dehydrogenase from bacillus stearothermophilus. hydroxylamine-, o-iodosobenzoic acid- and tryptic-fragments. the complete amino-acid sequence. | based on the partial sequence of the cyanogen bromide fragments [tratschin, j.d., wirz, b., frank, g. and zuber, h. (1983) hoppe-seyler's z. physiol. chem. 364, 879-892], the amino-acid sequence of thermophilic lactate dehydrogenase from b. stearothermophilus was completed by the preparation and sequencing (sequenator, carboxypeptidase a and y) of further overlapping fragments. suitable peptide fragments were obtained by lactate dehydrogenase cleavage with hydroxylamine, o-iodosobenzoic acid and ... | 1983 | 6352452 |
| [effect of mea on the degradation of thermally-damaged dna in gamma-irradiated bacterial cells]. | it was shown that the radioprotective action of beta-mercaptoethylamine on gamma-irradiated bacillus stearothermophilus, with regard to survival and diminution of dna breaks, depended upon the temperature of incubation and the presence of thermal lesions in dna. beta-mercaptoethylamine decreased the number of both alkali-labile sites and true single-strand dna breaks in cells of bacillus stearothermophilus. beta-mercaptoethylamine decreased the number of alkali--labile sites and true single-stra ... | 1983 | 6359242 |
| structural basis for the thermal stability of glyceraldehyde-3-phosphate dehydrogenases. | the amino acid sequences of two thermophilic and five mesophilic glyceraldehyde-3-phosphate dehydrogenases have been compared with the known three-dimensional structure of this enzyme to determine the factors responsible for thermal stability. the changes are greatest in the s-loop regions at the center of the tetramer, which show a quantitative increase in hydrophobicity and polarity that can strengthen subunit interactions in a complementary manner. the s-loops also show increases in residue v ... | 1983 | 6360926 |
| steam sterilization of air turbine dental handpieces. | the efficacy of autoclaving high-speed dental handpieces was tested in two types of downward displacement steam sterilizers (instrument autoclaves), commonly used in the dentist's office. eight series of experiments were performed with various sterilization schedules. lubrication oils with or without antimicrobial agent were used in four of the series. each handpiece was inoculated with 1 ml of a suspension containing equal amounts of saliva and spores of bacillus stearothermophilus (approx. 10( ... | 1983 | 6362319 |
| molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of bacillus stearothermophilus and escherichia coli, and their expression in escherichia coli. | recombinant plasmids derived from pbr322, which carry gap genes coding for the d-glyceraldehyde-3-phosphate dehydrogenases (gapdh) of bacillus stearothermophilus and escherichia coli, have been isolated. the selection was carried out by complementation of an e. coli gapam mutant. two plasmids containing b. stearothermophilus and e. coli dna inserts of 4.3 kb and 1.4 kb, respectively, were characterized. transformation of the e. coli mutant with either of the recombinant plasmids lead to a very h ... | 1983 | 6363208 |
| proteins of the bacillus stearothermophilus ribosome. the amino acid sequences of proteins s5 and l30. | the amino acid sequences of ribosomal proteins s5 and l30 from bacillus stearothermophilus have been determined. these proteins have recently been crystallized in our institute. sequence data were obtained by manual sequencing of peptides derived from cyanogen bromide cleavage and digestion with trypsin and chymotrypsin or thermolysin. proteins s5 and l30 contain 166 and 62 amino acid residues and have calculated mr values of 17,628 and 7,053, respectively. comparison of the sequences with those ... | 1984 | 6363400 |
| structural homology in the amino-terminal domains of two aminoacyl-trna synthetases. | the three-dimensional structures of two animoacyl-trna synthetases, the methionyl-trna synthetase from escherichia coli (metrs) and the tyrosyl-trna synthetase from bacillus stearothermophilus (tyrrs), show a remarkable similarity over a span of about 140 amino acids. the region of homologous folding corresponds to a five-stranded parallel beta-sheet, including a mononucleotide-binding fold. one cysteine and two histidine residues that were found to be invariant in the amino acid sequences occup ... | 1983 | 6363712 |
| the complete amino acid sequences of ribosomal proteins l17, l27, and s9 from bacillus stearothermophilus. | the complete primary structures of proteins l17, l27 and s9 extracted from the bacillus stearothermophilus ribosomes with 1 m nacl and purified to homogeneity by column chromatography have been determined. the amino acid sequences of these proteins are compared to those of the homologous ribosomal proteins from escherichia coli. the number of identical amino acid residues between the homologous proteins lies between 33-55%. | 1984 | 6365549 |
| [dependency of a microbiological test of a formaldehyde gas sterilization procedure on the shape of objects to be sterilized]. | during the last decade, a number of procedures have been developed by different firms for the sterilization of heat-sensitive instruments using a mixture of formaldehyde and water vapor at a temperature of approximately 60 degrees c as means of sterilization. instruments to be sterilized by this technique as e.g. sounds and catheters normally have long narrow cavities. therefore, the formaldehyde gas sterilization procedures have to be tested primarily for their capability of achieving a suffici ... | 1983 | 6367309 |
| a homogeneous enzyme immunoassay with avidin-ligand conjugate as the enzyme-modulator. | a homogeneous enzyme immunoassay (eia) based on the immunomodulation of an avidin-ligand conjugate and the inhibition of pyruvate carboxylase is described. the conjugation of the ligand, 5,5-diphenylhydantoin (dph), to avidin does not affect avidin's capacity to bind biotin or inhibit pyruvate carboxylase. the dph-avidin conjugate and free dph were shown to compete for a limited number of antibody sites. the interaction of anti-dph with the dph-avidin conjugate sterically inhibited enzyme inacti ... | 1984 | 6372543 |
| fractionation of dna with sephacryl s-1000. | in this study the application of gel filtration for purification of heterogeneous dna is described. the fractionation of partial restriction enzyme digests of bacterial chromosomal dna on a sephacryl s-1000 -column is easy and rapid. simultaneously intact chromosomal dna and low molecular weight substances are eliminated in the run. the method is also applicable to the purification of plasmid dna, as has been previously reported (3). thus we are able to get pure dna with yields over 80%. | 1984 | 6383398 |
| general stability of thermophilic enzymes: studies on 6-phosphogluconate dehydrogenase from bacillus stearothermophilus and yeast. | the thermophilic enzyme 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate:nadp oxidoreductase, decarboxylating, ec 1.1.1.44) from bacillus stearothermophilus was much more resistant to inactivation under different conditions of temperature, ph, guanidine-hydrochloride, and organic solvents (dioxane, dimethylformamide, acetone) than its mesophilic counterpart from yeast. in addition, the thermophilic enzyme largely withstands proteolysis with trypsin, chymotrypsin, and elastase when compare ... | 1984 | 6386790 |
| the primary structure of human liver manganese superoxide dismutase. | the complete amino acid sequence of manganese superoxide dismutase from human liver was determined. the sequence was deduced following characterization of the peptides obtained from tryptic, chymotryptic, and staphylococcus aureus digests of the apoprotein. chemical cleavage with dimethyl sulfoxide-hydrobromic acid was also carried out. the amino acid sequence listed below is made up of 196 amino acids and the two subunit polypeptides in the native enzyme appear to be identical. no homology was ... | 1984 | 6386798 |
| isolation, screening and characterization of thermophilic bacillus species isolated from dairy products. | proteolytic thermophilic bacterial cultures (171 strains) were isolated from different milk and milk products. after screening these isolates for protease production in a liquid medium, fifty that exhibited enzyme activity in excess of 100 units/ml were selected and identified. twenty-nine were bacillus stearothermophilus (constituting 58% of the total), twelve were b. coagulans, five were b. circulans and four were b. licheniformis. skim milk powder contributed the maximum number of b. stearoth ... | 1984 | 6389462 |
| specific sequence homology and three-dimensional structure of an aminoacyl transfer rna synthetase. | few and limited amino acid sequence homologies have been found among eight bacterial aminoacyl transfer rna (trna) synthetases whose primary structures are known. the entire 939-amino acid primary structure of escherichia coli isoleucyl-trna synthetase is now reported. in a sequence of 11 consecutive amino acids matching a sequence in e. coli methionyl-trna synthetase, there are ten identical residues and one conservative change. this is the strongest homology recorded between any two aminoacyl ... | 1984 | 6390679 |
| application of high-performance liquid chromatographic techniques to the separation of ribosomal proteins of different organisms. | the ribosomal proteins from escherichia coli, bacillus stearothermophilus and methanococcus vannielii were separated by size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography (hplc), employing new column materials, different gradient systems, and preparative columns, respectively. the purity of the isolated proteins was analysed by one- and two-dimensional gel electrophoresis and by direct micro-sequencing. the separation of ribosomal proteins could be improved by ... | 1984 | 6397476 |
| [glyceraldehydephosphate dehydrogenase]. | | 1984 | 6398871 |
| industrial prospects for thermophiles and thermophilic enzymes. | reasons for enzyme instability are discussed. thermophiles are a promising source of more stable intracellular enzymes. this aids their purification as well as providing desirable industrial properties. the organisms themselves have advantages for high temperature fermentations, e.g. for ethanol production. systems for cloning genes into them are under development. an example is given of genetic and physiological manipulation of a fast-fermenting bacillus stearothermophilus to increase ethanol y ... | 1983 | 6400480 |
| biological monitors. | | 1983 | 6404851 |
| on the insertion of proteins into membranes. | recent data concerning the primary structure and the interactions of proteins with membranes suggest the existence of two classes of integral membrane proteins. in the first class, the polypeptide chain crosses the membrane only once. the membrane penetrating fragment is markedly hydrophobic and contains several positive charges on its c-terminal border. in the second class, the protein is folded in a complex fashion within the membrane and the knowledge of its amino acid sequence is not suffici ... | 1983 | 6412767 |
| classification of iron-sulfur cores in ferredoxins by 1h nuclear magnetic resonance spectroscopy. | a 1h nuclear magnetic resonance (nmr) study was carried out on various ferredoxins which possess one of three types of iron-sulfur clusters, (2fe-2s), (3fe-3s), or (4fe-4s). in the isolated form, (2fe-2s) ferredoxins from spinach (spinacea oleracia), pokeweed (phytolacca americana), a blue-green alga (spirulina platensis), and a halobacterium (halobacterium halobium) exhibited two broad resonances common in chemical shift at the region downfield of 10 ppm. in their reduced forms, seven contact-s ... | 1983 | 6417123 |
| the amino acid sequence of two small ribosomal proteins from bacillus stearothermophilus. | the low-mr proteins (tentatively called protein i and ii) were purified from 2 m nacl extracts of the bacillus stearothermophilus ribosome. their amino acid sequences have been determined from the peptides obtained by digestion with trypsin, chymotrypsin, and pepsin, and by cleavage with cnbr, using the micro-dabitc/pitc double-coupling method [febs lett. (1978) 93, 205-214]. protein i contains 56 residues and has an mr of 6514. protein ii had 37 residues with an mr of 4361. the amino acid seque ... | 1984 | 6420194 |
| synthesis of peptidoglycan by high molecular weight penicillin-binding proteins of bacillus subtilis and bacillus stearothermophilus. | the high molecular weight penicillin-binding proteins (pbp(s) ) bacillus subtilis pbps 1, 2, and 4 and bacillus stearothermophilus pbps 1-4 were shown to catalyze peptidoglycan synthesis from the undecaprenol-containing lipid intermediate substrate in two assay systems. in a filter paper assay system, high levels of substrate polymerization occurred when reaction mixtures were incubated on whatman 3mm filter paper. the ph optimum for peptidoglycan synthesis was 7.5 for b. subtilis pbps 1, 2, and ... | 1984 | 6420410 |
| domain structure and 1h-n.m.r. spectroscopy of the pyruvate dehydrogenase complex of bacillus stearothermophilus. | the pyruvate dehydrogenase complex of bacillus stearothermophilus was treated with staphylococcus aureus v8 proteinase, causing cleavage of the dihydrolipoamide acetyltransferase polypeptide chain (apparent mr 57 000), inhibition of the enzymic activity and disassembly of the complex. fragments of the dihydrolipoamide acetyltransferase chains with apparent mr 28 000, which contained the acetyltransferase activity, remained assembled as a particle ascribed the role of an inner core of the complex ... | 1984 | 6421282 |
| methylation of ribosomal proteins in bacteria: evidence of conserved modification of the eubacterial 50s subunit. | methylation of the 50s ribosomal proteins from bacillus stearothermophilus, bacillus subtilis, alteromonas espejiana, and halobacterium cutirubrum was measured after the cells were grown in the presence of [1-14c]methionine or [methyl-3h]methionine or both. two-dimensional polyacrylamide gel electrophoretic analysis revealed, in general, similar relative electrophoretic mobilities of the methylated proteins from each eubacterium studied. proteins known to be structurally and functionally homolog ... | 1984 | 6425271 |
| rapid assay for tetracycline in premixes and mixed feeds. | a rapid assay for tetracyclines in premixes and mixed feeds is described, which uses the extraction and dilution systems of aoac methods, and a strain of bacillus stearothermophilus atcc 12980 selected to grow at 40 degrees c. the incubation period is 4.5 h. the rapid assay yields results similar to those obtained using the aoac methods. for a 50 g chlortetracycline (ctc)/lb commercial premix, the rapid procedure averaged 109.2% of label vs 104.4% obtained using the aoac method; for a 20 g ctc/l ... | 1984 | 6430864 |
| identification of bacillus strains using the api system. | a system is described for the rapid and accurate identification of bacillus isolates using a matrix of results from tests in the api 20e and api 50chb strips and from supplementary tests. api system tests have been shown to be more reproducible than the classical tests. a taxonomy based upon api tests is in good agreement with those obtained by other methods. the results matrix can also be used in computer assisted identification. | 1984 | 6432953 |
| molecular basis of isozyme formation of beta-galactosidases in bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaa and bgab. | bacillus stearothermophilus iam11001 produced three beta-galactosidases, beta-galactosidase i, ii, and iii (beta-gal i, ii, and iii), which are detectable by polyacrylamide (nondenatured) gel electrophoresis. by connecting restriction fragments of the chromosomal dna to plasmid vectors, followed by transformation of escherichia coli, two beta-galactosidase genes (bgaa and bgab) located close to each other on the chromosome were isolated. identification of the gene products and southern hybridiza ... | 1984 | 6434528 |
| antimicrobial residues in milk. comparison of different agar diffusion methods. | | 1984 | 6439018 |
| [disinfection and sterilization of thermolabile instruments with gaseous formaldehyde]. | for several years formaldehyde-gas-underpressure procedures are increasingly used for disinfection and sterilization of medical thermolabile instruments. in many places, however, uncertainty and sceptism, if these methods are sufficient in the medical field, are existing. this is understandable, since no generally test instructions are available. the principal suitability of gaseous formaldehyde for disinfection and sterilization, however, had been demonstrated by several investigators. precondi ... | 1984 | 6442506 |
| [efficiency and effect of 3 systems of sterilization of periodontal instruments]. | | 1984 | 6443139 |
| amino acid sequence at the allosteric site of sheep heart phosphofructokinase. | covalent modification of sheep heart phosphofructokinase with the affinity labeling reagent p-fluorosulfonyl[14c]benzoyl-5'-adenosine caused a loss of allosteric properties. this modification appears to occur at the binding site that is specific for the allosteric activators amp, camp, and adp (mansour, t.e., and colman, r.f. (1978) biochem. biophys. res. commun. 81, 1370-1376). in the current study, the site of modification has been demonstrated to be a lysine residue. a nonapeptide containing ... | 1980 | 6444412 |
| phosphofructokinase: complete amino-acid sequence of the enzyme from bacillus stearothermophilus. | the entire amino acid sequence of the protein subunit of phosphofructokinase from bacillus stearothermophilus has been established mainly by sequence analysis of cyanogen bromide fragments and of peptides derived from these fragments by further digestion with proteolytic enzymes. overlaps of the cyanogen bromide fragments as well as peptide sequences necessary to complement and to confirm tentative assignments within the larger peptide fragments were obtained from the sequences of selected pepti ... | 1980 | 6447595 |
| phosphofructokinases from lactobacteriaceae. ii. purification and properties of phosphofructokinase from streptococcus thermophilus. | phosphofructokinase (atp : d-fructose-6-phosphate 1-phosphotransferase, ec 2.7.1.11) from streptococcus thermophilus has been purified. it is a tetramer composed of identical subunits of molecular weight 36 000 and exhibits michaelis-menten kinetics. compared to the phosphofructokinases from taxonomically related bacteria, the enzyme from s. thermophilus is more stable at high temperatures. in addition, it has been demonstrated that the phosphofructokinases from lactobacteria and also from baci ... | 1981 | 6457643 |
| the stereochemical course of phosphoryl transfer catalysed by bacillus stearothermophilus and rabbit skeletal-muscle phosphofructokinase with a chiral [16o,17o,18o]phosphate ester. | bacillus stearothermophilus and rabbit skeletal-muscle phosphofructokinases catalyse the transfer of the chiral [16o,17o,18o]phosphoryl group from d-fructose 1[(s)-16o,17o,18o],6-bisphosphate to adp with inversion of configuration at the phosphorus atom. d-fructose 1[(s)-16o,17o,18o],-bisphosphate was synthesized in situ from sn-glycerol 3[(s)-16o,17o,18o]phosphate. the simplest interpretation of these results is that the phosphoryl group is transferred between substrates in the enzyme substrate ... | 1981 | 6462132 |
| cold lability and dissociation of the f1-atpase from bacillus stearothermophilus. | | 1982 | 6462141 |
| effect of mercaptoethylamine on dna degradation in thermophilic bacteria bac. stearothermophilus exposed to gamma-, uv-radiation or methylnitrosourea. | the effect of mercaptoethylamine (mea) on degradation of dna in thermophilic bacteria bac. stear. exposed to gamma-, uv-rays or methylnitrosourea (mnu) was studied. using centrifugation on alkaline and neutral sucrose gradients, it was shown that mea inhibits the accumulation of breaks in the dna of bac. stear. it also lowers the level of dna degradation in toluene-treated cells of bac. stear. under the action of the intrinsic nuclease, reduces the activity of the endonuclease specific for apuri ... | 1984 | 6473706 |
| the use of double mutants to detect structural changes in the active site of the tyrosyl-trna synthetase (bacillus stearothermophilus). | in a previous study, a mutant of tyrosyl-trna synthetase in which a threonine residue (thr51) was converted to proline dramatically improved the affinity of the enzyme for its atp substrate. how does pro51 improve the enzyme's affinity for atp? a priori, pro51 might interact directly with the atp, or it might distort the polypeptide backbone and thereby force new or improved contacts elsewhere from the enzyme to atp. by making mutants of the pro51 enzyme at two residues that make hydrogen bonds ... | 1984 | 6488318 |
| construction of a system for the regeneration of adenosine 5'-triphosphate, which supplies energy to bioreactor. | an engineering model was successfully developed for an atp regeneration system by using two enzymes, acetate kinase (ak) and adenylate kinase (adk), both obtained from the thermophile bacillus stearothermophilus. this model is composed of five units: a substrate unit consisting of substrate solutions--amp, atp, and acetyl phosphate (acop)--an enzymatic reactor unit consisting of ak and adk immobilized to sepharose 4b, an auto sampler unit, an analytical unit made up of high-performance liquid ch ... | 1984 | 6490579 |
| structural evidence for ligand-induced sequential conformational changes in glyceraldehyde 3-phosphate dehydrogenase. | glyceraldehyde 3-phosphate dehydrogenase is a tetramer of four chemically identical subunits which requires the cofactor nicotinamide adenine dinucleotide (nad) for activity. the structure of the holo-enzyme from bacillus stearothermophilus has recently been refined using x-ray data to 2.4 a resolution. this has facilitated the structure determination of both the apo-enzyme and the enzyme with one molecule of nad bound to the tetramer. these structures have been refined at 4 a resolution using t ... | 1984 | 6492162 |
| bioequivalent chemical steam sterilization indicators. | biological indicators used to monitor steam sterilization cycles have two major shortcomings--the incubation period needed to determine if sterilization was accomplished, and the reliance on test packs for gathering information in each load. chemical indicators do not suffer from these shortcomings. chemical indicators can respond to time, temperature, and steam parameters to thus parallel the bi reaction. nine commercially available chemical indicators and four biological indicators were evalua ... | 1984 | 6493101 |
| crystallization of substrate and product analog complexes of tryptophanyl-trna synthetase. | we have prepared crystals of tryptophanyl-trna synthetase from bacillus stearothermophilus complexed to tryptophan (type ii*), and to tryptophanyl-3'(2')-atp (type iv). the latter compound is a product analog, enzymatically synthesized by acyl transfer of tryptophan from the tryptophanyl-5'-amp intermediate to a second molecule of atp. it resembles the 3'-terminal fragment, tryptophanyl-3'(2')-adenosine, of trp-trnatrp. both crystal forms diffract to high resolution. although both forms are grow ... | 1984 | 6500072 |
| [what should be the length and inner diameter of the testing device for microbiological efficacy testing of formaldehyde gas sterilization methods?]. | the series of tests described in a preceding publication (spicher and borchers, 1983) has been continued in a modified way. this time, the dependency of the microbiological test results of a formaldehyde gas sterilization procedure on length and inner diameter of the tubes serving as test pieces was examined. the tubes were 1 or 2 m in length with an inner diameter of 1 or 2 mm. the tests were performed with four different preparations of bioindicators. spores of bac. stearothermophilus served a ... | 1984 | 6524142 |
| enzymatically active subunits of bacillus stearothermophilus enolase bound to sepharose. | the octameric enolase from bacillus stearothermophilus was immobilized onto sepharose 4b activated by the cyanogen bromide reaction under conditions for achieving essentially a single-point attachment. the immobilized enzyme was dissociated with guanidine hydrochloride to yield bound monomeric enolase. the sepharose-bound subunit regained activity upon removal of the denaturant. it was also possible to rehydribize immobilized monomers to native octamers. of note, the thermal stability of the imm ... | 1984 | 6530331 |
| residues in colostrum following antibiotic dry cow therapy. | cows from five dairy herds were used to determine persistence of antibiotic residues in colostrum and milk following dry cow therapy. cows were treated in all quarters at drying off with antibiotics approved for use for nonlactating cows. antibiotics procaine penicillin g plus dihydrostreptomycin, novobiocin, cloxacillin, or cephapirin were compared with no treatment. composite colostrum samples were collected from each cow at first milking after parturition. samples were screened for residues b ... | 1984 | 6530498 |
| 3-a resolution structure of a protein with histone-like properties in prokaryotes. | the 3-a structure of dna-binding protein ii, which exhibits histone-like properties in bacteria, has been determined. the molecule is dimeric and appears to bind to the phosphate backbone of dna through two symmetry-related arms. a mechanism by which the protein induces dna supercoiling is proposed. | 1984 | 6540370 |
| high pressure dissociation of lactate dehydrogenase from bacillus stearothermophilus and reconstitution of the enzyme after denaturation in 6 m guanidine hydrochloride. | tetrameric lactate dehydrogenase from bacillus stearothermophilus exhibits unusual stability towards high hydrostatic pressure: in contrast to the mesophilic enzyme, incubation at pressures up to 2.8 kbar does not cause irreversible denaturation. hybridization under these conditions suggests partial dissociation to the dimer, indicating that reassociation occurs within the dead-time after pressure release (less than 20 s at less than or equal to 40 micrograms/ml, 20 degrees c). incubation at p l ... | 1984 | 6544680 |
| sequence of trnaleu cmaa from bacillus stearothermophilus. | the primary structure of bacillus stearothermophilus trnaleu was determined and found to be :pgccgaugs4uggcggaaudggcagm1acgcgcacgacucmaams2i6aa psi cgugugggcuuugcccgugugggt psi cgacucccaccaucggcacca. the molecule has a large extraloop and contains only 8 minor nucleotides. there is a g at position 21 like in all other sequenced bacterial trnasleu.m1a is in position 22, just before the d stem like in several other procaryotic trnas. the anticodon is cmaa and is adjacent to a ms2i6a in the 3'-dire ... | 1983 | 6552187 |
| crystals of bacillus stearothermophilus tryptophanyl-trna synthetase containing enzymatically formed acyl transfer product tryptophanyl-atp, an active site maker for the 3' cca terminus of tryptophanyl-trnatrp. | it has previously been shown that tryptophanyl-trna synthetase from bacillus stearothermophilus crystallizes in different forms, depending on the substrates present during crystallization [carter, c. w., jr., & carter, c. w. (1979) j. biol. chem. 254, 12219-12223]. radiolabeling experiments show that the tetragonal crystals (type iv), grown in the presence of tryptophan and atp, contain enzymatically formed 3'(2')-tryptophanyladenosine 5'-triphosphate (trp-atp). trp-atp is formed by acyl transfe ... | 1984 | 6559601 |
| kasugamycin resistant mutants of bacillus stearothermophilus lacking the enzyme for the methylation of two adjacent adenosines in 16s ribosomal rna. | several mutants of b. stearothermophilus have been isolated that are resistant to the antibiotic kasugamycin. one of these is shown to lack dimethylation of two adjacent adenosines in the 16s ribosomal rna. all mutants that were analyzed biochemically lack the enzyme that is able to methylate this site. ribosomal sensitivity and resistance to kasugamycin in b. stearothermophilus is therefore, like in e. coli, closely connected with dimethylation of the adenosines. | 1983 | 6575236 |
| transformation of bacillus stearothermophilus with plasmid dna and its application to molecular cloning. | | 1983 | 6584074 |
| [the reliability of the dry-heat sterilizer]. | | 1984 | 6590983 |
| [effect of mea on the accumulation of dna breaks in bac. stearothermophilus irradiated with gamma and ultraviolet rays and treated with nitrosomethylurea]. | beta-mercaptoethylamine (mea) decreased the accumulation of enzymatic single- and double-strand breaks in dna of thermophilic bacteria exposed to gamma- and uv-radiation and treated with n-nitroso-n-methylurea. the protective effect of mea, as registered according to accumulation of single-strand and double-strand breaks in dna of bac. stearothermophilus immediately after irradiation and after 30 min postirradiation incubation, was similar. | 1983 | 6611869 |
| activation and germination characteristics observed in endospores of thermophilic strains of bacillus. | the properties of endospores of some thermophilic strains of bacillus were examined. included were strains isolated from thermal pools and springs in yellowstone national park, a strain of b. thermodenitrificans and two strains of b. stearothermophilus, atcc 7953 (smooth) and t-10. the spores of thermophilic strains of bacillus contained relatively high levels of dipicolinic acid ranging from 11-14.8% of the spore dry weight, while the calcium levels were similar to those observed in other bacte ... | 1983 | 6615124 |
| site-directed mutagenesis as a probe of enzyme structure and catalysis: tyrosyl-trna synthetase cysteine-35 to glycine-35 mutation. | oligodeoxynucleotide-directed mutagenesis has been used on the gene of tyrosyl-trna synthetase from bacillus stearothermophilus to produce mutant enzymes altered at the adenosine 5'-triphosphate (atp) binding site. deliberate attempts were made to alter rather than destroy enzymic activity so that kinetic measurements may be made to identify the subtle roles of the enzyme-substrate interactions in catalysis. cys-35, the -sh group of which is involved in binding the 3'-oh of the ribose ring of at ... | 1983 | 6615786 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria. ii) the primary structure of thermophilic lactate dehydrogenase from bacillus stearothermophilus. cyanogen bromide fragments and partial sequence. | the polypeptide chain of thermophilic lactate dehydrogenase from bacillus stearothermophilus was split with cyanogen bromide. the 6 cyanogen bromide fragments were then separated and isolated by gel filtration (bio-gel p 10, sephadex g-75) and ionic exchange chromatography (biorex 70), respectively. peptide fractionation was performed in 50% formic acid. fragment yield varied between 30 and 75%. about 75% of the amino-acid sequence was determined by the automatic n-terminal sequence analysis (am ... | 1983 | 6618448 |
| a compact three-dimensional crystal form of the large ribosomal subunit from bacillus stearothermophilus. | a new form of well-ordered three-dimensional crystals of intact 50 s ribosomal subunits from bacillus stearothermophilus have been obtained. electron micrographs of positively stained sections of these crystals revealed that the ribosomal particles are packed closely. the cell parameters have been determined. representative electron micrographs and their computed contoured filtered images are shown. | 1983 | 6628691 |
| proteins of the bacillus stearothermophilus ribosome. a 5 a structure analysis of protein s5. | the structure of protein s5 from the small subunit of the bacillus stearothermophilus ribosome is described to a resolution of 5 a. the molecular boundary is visible and shows the protein to be essentially compact although slightly elongated in one dimension. | 1983 | 6628692 |