| postpartum ovarian follicular dynamics in primiparous and pluriparous mediterranean italian buffaloes (bubalus bubalis). | the objective of this study was to monitor ovarian function in postpartum primiparous and pluriparous mediterranean italian buffaloes (bubalus bubalis) during months of increasing daylength. ovarian ultrasound monitoring was carried out for a total of 60 days from calving in 10 primiparous and 10 pluriparous buffaloes. progesterone was determined from calving until a week after first postpartum ovulation. the study was undertaken during months of increasing day length. time required for complete ... | 2005 | 15725449 |
| platelet activating factor improves the in vitro penetration of zona free hamster eggs by buffalo (bubalus bubalis) spermatozoa. | twelve buffalo bulls of murrah breed, selected on the basis of their conception rates, were classified into low-, moderate- and high-fertility groups. frozen semen was thawed and treated with 200 microm platelet activating factor (paf) for 15 min at 37 degrees c and 5% co2. in both treated and control (no paf) semen samples (five replicates per bull), the following were assessed: motility, acrosome reaction (ar) evaluation (for 10 replicates of each bull), and zona-free hamster oocyte penetratio ... | 2005 | 15763101 |
| cloning and sequencing of indian water buffalo interleukin-18 cdna. | summary full-length cdna (582 bp) of the interleukin-18 (il-18) gene of the indian water buffalo (bubalus bubalis) was amplified by reverse transcriptase-polymerase chain reaction (rt-pcr) and sequenced. the deduced amino acid sequence has 99% and 95% similarity with the il-18 sequences of cattle and sheep, respectively. there are two amino acid substitutions at positions 132 and 182 in buffalo il-18 compared with that of cattle. phylogenetic analysis showed that the il-18 sequence of fish forms ... | 2005 | 15787638 |
| isolation of verocytotoxin-producing escherichia coli o157 from water buffaloes (bubalus bubalis) in southern italy. | | 2005 | 15816184 |
| serological responses to neospora caninum in experimentally and naturally infected water buffaloes (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important natural host for neospora caninum. serologic responses to n. caninum were studied in experimentally and naturally infected water buffaloes in brazil. antibodies were assayed by the indirect fluorescent antibody test (ifat) using a cut off value of 1:25. six buffaloes were each inoculated subcutaneously with 5 x 10(6) live culture-derived tachyzoites of the cattle illinois strain of n. caninum, and two calves were kept as uninoculated controls. ... | 2005 | 15817198 |
| ovum pick-up in cycling and lactating postpartum swamp buffaloes (bubalis bubalis). | the objective of this study was to evaluate the efficiency of ovum pick up (opu) in cycling (n = 5) and lactating, postpartum, swamp buffaloes (n = 6) with and without gonadotropin stimulation. the opu was performed every two weeks in all groups of animals, for a total of six sessions. thirty collections were performed in five cycling buffaloes and 36 collections in six lactating postpartum buffaloes. buffaloes that received hormonal stimulation were given a total of 400 mg, follicle stimulating ... | 2005 | 15819965 |
| conception rate after fixed time insemination following ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic mediterranean italian buffaloes (bubalus bubalis). | the aim of this study was to test the effect of progesterone supplementation to ovsynch protocol in cyclic and non-cyclic mediterranean italian buffaloes on conception rate after fixed time artificial insemination. from 169 pluriparous buffaloes, 2 groups were identified and subjected to: (1) ovsynch protocol (ov; n=83) and (2) ovsynch protocol with the supplementation of progesterone from days 0 to 7 (ov+prog.; n=86). all cows were inseminated 16-20 h after the second gnrh administration. withi ... | 2005 | 15823341 |
| expression of leptin and its receptors in various tissues of ruminants. | the energy metabolism of domestic animals is under the control of hormonal factors, which include thyroid hormones and leptin. leptin signals from the periphery to the centre. it is mostly produced in the white adipose tissue and informs the central nervous system (cns) about the total fat depot of the body. low and high levels of leptin induce anabolic and catabolic processes, respectively. besides controlling the food uptake and energy expenditure leptin is also involved in regulation of the r ... | 2005 | 15878255 |
| detection of mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (bubalus bubalis) by pcr and bacterial culture. | the efficacy of bacterial culture and is900-specific polymerase chain reaction (pcr) was compared for the detection of mycobacterium avium subsp. paratuberculosis (map) from the intestinal and mesenteric lymph node tissues of water buffaloes (bubalus bubalis) showing lesions of paratuberculosis (johne's disease). out of 20 (4.9%) animals showing histological lesions suggestive of paratuberculosis, 14 (70%) and 6 (30%) were positive in the pcr and bacterial culture, respectively. the results of t ... | 2005 | 15885931 |
| haematological profile on non-lactating mediterranean buffaloes (bubalus bubalis) ranging in age from 24 months to 14 years. | haematological studies were performed on 100 clinically normal non-lactating mediterranean buffalo species ranging in age from 24 months to 14 years, to determinate the range of normal haematological values for this ruminant species. the animals were divided in 5 groups according to age: group i, 2-3 years old which had never calved, group ii, 3-4 years old (primipara buffaloes), group iii, 5-7 years old, group iv 8-10 years old and group v over 10 years of age. all the haematological values obt ... | 2004 | 15894028 |
| influence of epidermal growth factor and insulin-like growth factor 1 on nuclear maturation and fertilization of buffalo cumulus oocyte complexes in serum free media and their subsequent development in vitro. | the in vitro maturation, fertilization and development of indian water buffalo (bubalus sp.) cumulus oocyte complexes (cocs) to blastocysts were studied during culture, either in serum free tissue culture medium 199 (tcm 199) or waymouth mb (wm). based on different supplements added to these media, the experimental groups included: (a) no supplement (control); (b) hormones (fsh, lh and oestradiol) (c) epidermal growth factor (egf); (d) igf-1; and (e) egf + igf-1. experiments were conducted to no ... | 2004 | 15911173 |
| repeated ultrasound-guided transvaginal oocyte retrieval from cyclic murrah buffaloes (bubalus bubalis): oocyte recovery and quality. | the present study was undertaken to explore the potential of the murrah breed of buffaloes as donors of oocytes and to find out the recovery rate and oocyte quality in cyclic murrah buffaloes subjected to oocyte recovery once a week. murrah buffaloes (n = 5) were synchronized for estrus by a single prostaglandin injection schedule. the animals were subjected to transvaginal oocyte retrieval (tvor) once weekly for 6 weeks, starting from day 7 of the oestrous cycle (day 0 = day of oestrus). tvor w ... | 2006 | 15913926 |
| interleukin-12 subunits p35 and p40 of indian water buffalo (bubalus bubalis) maintain high sequence homology with those of other ruminants. | the immune system of indian water buffalo (bubalus bubalis), one of the major dairy animals of the tropics, has received little attention. cdnas encoding the two subunits of the heterodimeric interleukin (il)-12 of indian water buffalo were isolated from concanavalin a-stimulated lymphocytes. the 710-bp p35 and 1012-bp p40 subunits were amplified by reverse transcriptase polymerase chain reaction (rt-pcr), cloned, sequenced and compared with other ruminant sequences. the il-12 p35 subunit cdna h ... | 2005 | 15932623 |
| characterisation of a cryptosporidium isolate from water buffalo (bubalus bubalis) by sequencing of a fragment of the cryptosporidium oocyst wall protein gene (cowp). | cryptosporidium oocysts were detected using a direct immunofluorescence antibody test in the faeces of an asymptomatic water buffalo (bubalus bubalis) heifer from a dairy farm close to santiago de compostela (nw spain). oocysts were morphologically indistinguishable from cryptosporidium parvum. using dna extracted from this sample and a pcr-rflp analysis of a 341 base pairs fragment of the cryptosporidium oocyst wall protein (cowp) gene, a previously undescribed fragment pattern was generated. t ... | 2005 | 15935559 |
| the detection and pcr-based characterization of the parasites causing trypanosomiasis in water-buffalo herds in venezuela. | the usefulness of pcr-based assays for detecting trypanosomiasis in water buffaloes and other livestock was explored, under field conditions, in venezuela. the sensitivity and specificity of the assays, which were based on established primer pairs (21-mer/22-mer and ilo1264/ilo1265), were evaluated, partly by comparison with the results of parasitological tests (stained bloodsmears and microhaematocrit centrifugation) and immunological assays (ifat) run in parallel. the optimised pcr-based assay ... | 2005 | 15949183 |
| effect of long term feeding of ammoniated wheat straw treated with or without hcl on blood biochemical parameters in growing male buffalo (bubalus bubalis) calves. | twenty-four growing male buffalo calves (one year of age; 88.54 +/- 3.81 kg average body weight) were divided into three comparable groups (i, ii and iii) on the basis of their body weight (bw) in a completely randomised design to study the effect of long term feeding of ammoniated wheat straw (aws) and hydrochloric acid treated ammoniated wheat straw (hcl-aws) on blood biochemical changes. the animals were offered a concentrate mixture (cm) along with wheat straw (ws), ammoniated wheat straw (a ... | 2005 | 15952422 |
| evaluation of cell-mediated immune responses and bacterial clearance in 6-10 months old water buffalo (bubalus bubalis) experimentally vaccinated with four dosages of commercial brucella abortus strain rb51 vaccine. | thirty water buffalo, obtained from a brucellosis-free farm, were used to evaluate cell-mediated immune responses and bacterial clearance in response to vaccination with brucella abortus strain rb51 (rb51) in a dose-response study. the animals were randomly divided into five treatment groups. groups i--v received the recommended dose (rd) of rb51 vaccine once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart and saline once, respectively. cell-mediated immune response to rb5 ... | 2005 | 15963819 |
| effects of long-term growth hormone-releasing factor treatment on growth, feed conversion efficiency and dry matter intake in growing female buffaloes (bubalus bubalis). | effects of long-term growth hormone-releasing factor (grf) on growth performance, feed conversion efficiency (fce) and dry matter intake (dmi) were studied in growing buffaloes. twelve female murrah buffaloes of 6-8 months of age were divided into two groups of six each on the basis of their body weights so that the average body weights of the groups did not differ (p > 0.05). animals of each group were administered intravenously with either sterile distilled water (control group) or equal volum ... | 2005 | 15972076 |
| chromosome evolution and improved cytogenetic maps of the y chromosome in cattle, zebu, river buffalo, sheep and goat. | comparative fish-mapping among y chromosomes of cattle (bos taurus, 2n = 60, bta, submetacentric y chromosome), zebu (bos indicus, 2n = 60, bin, acrocentric y chromosome but with visible small p-arms), river buffalo (bubalus bubalis, 2n = 50, bbu, acrocentric y chromosome), sheep (ovis aries, 2n = 54, oar, small metacentric y chromosome) and goat (capra hircus, 2n = 60, chi, y-chromosome as in sheep) was performed to extend the existing cytogenetic maps and improve the understanding of karyotype ... | 2005 | 15973500 |
| enrichment of in vitro maturation medium for buffalo (bubalus bubalis) oocytes with thiol compounds: effects of cystine on glutathione synthesis and embryo development. | the purpose of this study was to evaluate whether enriching the oocyte in vitro maturation medium with cystine, in the presence of cysteamine, would improve the in vitro embryo production efficiency in buffalo by further increasing the gsh reservoir created by the oocyte during maturation. cumulus-oocytes complexes were matured in vitro in tcm 199 + 10% fcs, 0.5 microg/ml fsh, 5 microg/ml lh and 1 microg/ml 17beta-estradiol in the absence or presence of cysteamine (50 microm), with or without 0. ... | 2006 | 15979699 |
| vaccination of buffaloes with fasciola gigantica recombinant fatty acid binding protein. | recombinant fatty acid binding protein (rfabp) of fasciola gigantica was expressed in escherichia coli and used as vaccine in freund's adjuvant to evaluate the level of protection induced in buffalo (bubalus bubalis) calves. fifteen buffalo calves were distributed to three groups of five calves each. an antigen dose of 400 mug for each of the three immunizations at 3-week intervals, and a challenge dose of 600 metacercariae was administered per calf. levels of anti-fabp antibodies increased rapi ... | 2005 | 15986249 |
| molecular cloning and expression profile analysis of interleukin-10 and interleukin-18 cdna of indian water buffalo (bubalus bubalis). | the cdnas encoding the interleukin-10 and interleukin-18 of indian water buffalo (bubalus bubalis) were cloned and sequenced. a 537 bp il-10 cdna fragment and a 623 bp il-18 cdna fragment were amplified by reverse transcriptase polymerase chain reaction (rt-pcr) from concanavalin a stimulated splenocytes. sequence analysis of these cytokines revealed high level conservation at nucleic acid and protein level. both these cytokines also showed strict conservation in the predicted secondary structur ... | 2005 | 15990173 |
| pcr-rflp analysis: a promising technique for host species identification of blood meals from tsetse flies (diptera: glossinidae). | a polymerase chain reaction with the restriction fragment length polymorphism (pcr-rflp) method using universal primers complementary to the conserved region of the cytochrome b gene (cyt b) of the mitochondrion dna (mtdna) of vertebrates was applied to the identification of the origin of blood meals in tsetse flies. blood samples from ten potential tsetse hosts of the family bovidae (cattle, water buffalo, red buffalo, waterbuck, springbok, goat, sheep, sable antelope, oryx and dik-dik) were in ... | 2005 | 15999278 |
| comparative mapping of the fragile histidine triad (fhit) gene in cattle, river buffalo, sheep and goat by fish and assignment to bta22 by rh-mapping: a comparison with hsa3. | | 2005 | 16026356 |
| malignant catarrhal fever in a swamp buffalo (bubalus bubalis) calf in new zealand. | malignant catarrhal fever was diagnosed in a newly imported swamp buffalo calf. this disease is prevalent in deer and cattle in new zealand and causes sporadic losses in these species. the disease is recognised as a problem in buffaloes overseas and this paper records the first case in a swamp buffalo in new zealand. | 1993 | 16031692 |
| expression of gap junction protein connexin 43 during follicular atresia in the ovary of swamp buffaloes. | the present study was performed to detect the presence of gap junction protein connexin 43 (cx43) and describe the changes in its expression during ovarian follicular atresia in the swamp buffalo in comparison with cattle. ovaries of philippine swamp buffaloes (bubalus bubalis; sb) and holstein-friesian cows (bos taurus; hf) were collected from slaughterhouses, fixed in 10% formalin in pbs and embedded in paraffin. sections of healthy follicles and at various follicular stages of atresia were im ... | 2005 | 16034196 |
| molecular cloning and characterization of beta-defensin cdna expressed in distal ileum of buffalo (bubalus bubalis). | defensins play a prominent role in protection of various epithelial surfaces. in this study, we have cloned and characterized the mrna from the distal ileum of bubalus bubalis. total rna after isolation from ileal epithelium was reverse transcribed to synthesize cdna using primers designed by taking conserved region of cattle enteric beta-defensin (ebd) mrna, goat beta-defensin 2 (bd 2) and cattle lingual antimicrobial peptide (lap) mrna sequences. the pcr amplified cdna of 254 bp was ligated to ... | 2005 | 16040342 |
| is one-wave follicular growth during the estrous cycle a usual phenomenon in water buffaloes (bubalus bubalis)? | the pattern of growth and regression of ovarian follicles was monitored once daily for one complete estrous cycle in eight individual water buffaloes by ultrasonographic scanning of the ovaries for an entire interovulatory interval of normal cycle length. one-wave follicular growth was observed in five animals and two-wave follicular growth in three buffaloes during the estrous cycle. the first follicular wave of a two-wave cycle emerged significantly earlier (p < 0.05) than the emergence of the ... | 2006 | 16046086 |
| occurrence of anti-neospora caninum antibodies in water buffaloes (bubalus bubalis) from the northern region of brazil. | anti-neospora caninum antibodies were determined in sera of 196 water buffaloes (bubalus bubalis) from three farms of the northern region of brazil, using an indirect fluorescent antibody test. antibody titers were found in 139 (70.9%) buffaloes with dilution values ranging from > or =25 to <200 in 41 animals, > or =200 to <800 in 35 animals, and > or =800 in 63 animals. the number of animals presenting titers > or =800 was statistically higher (p<0.05). all farms presented positive animals, how ... | 2005 | 16051440 |
| buffalo (bubalus bubalis) interleukin-12: analysis of expression profiles and functional cross-reactivity with bovine system. | interleukin-12, a heterodimeric pro-inflammatory cytokine, from water buffaloes (bubalus bubalis) was analyzed for its for its tissue specific expression and functionality. concanavalin a stimulated splenocytes displayed an up-regulation of the il-12 p40 subunit 8-24h post-stimulation, whereas the p35 subunit did not show any quantitative variation at different time intervals. basal level expressions of both the subunits were observed by rt-pcr in spleen. in addition p40 transcripts could be det ... | 2006 | 16112195 |
| serologic responses, biosafety and clearance of four dosages of brucella abortus strain rb51 in 6-10 months old water buffalo (bubalus bubalis). | thirty water buffalo were obtained from a brucellosis-free farm in order to evaluate antibody responses, bacterial clearance and safety to brucella abortus strain rb51 vaccine in a dose response study. the animals were randomly divided into five treatment groups. groups i-v received the recommended dose of rb51 vaccine (rd) once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart and saline once, respectively. antibody responses to rb51 were monitored at 2, 4, 6, 8, 10, 12, 16 ... | 2006 | 16112746 |
| efficacy of the ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in murrah buffaloes (bubalus bubalis). | two experiments were conducted to assess the timing and synchrony of ovulation, plasma lh concentrations, and pregnancy rate in murrah buffaloes (bubalus bubalis) treated with the ovsynch (gnrh-pgf(2 alpha)-gnrh) protocol. in experiment 1, 10 non-lactating cycling buffaloes received 10 microg of a gnrh analogue i.m. (buserelin acetate) without regard to the stage of the estrous cycle (day of treatment, day 0), followed by 25mg of pgf(2 alpha) i.m. (dinoprost thromethamine) 7 days later. a second ... | 2005 | 16125551 |
| prospects for development of a transmission blocking vaccine against schistosoma japonicum. | despite intensive long-term control programmes, schistosomiasis japonica remains a serious public health problem in china and the philippines. the termination of mass praziquantel-treatment has seen a dramatic recent rebound in both its prevalence and associated morbidity. schistosomiasis japonica is a zoonosis but, despite complicating control efforts, this feature provides a practical method for attacking schistosoma japonicum through development and deployment of a transmission blocking veter ... | 2005 | 16138851 |
| development of embryos reconstructed by interspecies nuclear transfer of adult fibroblasts between buffalo (bubalus bubalis) and cattle (bos indicus). | the objective of this study was to explore the feasibility of employing adult fibroblasts as donor cells in interspecies nuclear transfer (nt) between buffaloes and cattle. buffalo and bovine oocytes matured in vitro for 22 h were enucleated by micromanipulation using the spindle view system. an ear fibroblast, pretreated with 0.1 microg/ml aphidicolin for 24 h, followed by culture for 2-9 days in dulbecco's modified eagle's media+0.5% fetal bovine serum, was introduced into the cytoplast by mic ... | 2005 | 16139607 |
| follicle turnover and pregnancy rates following oestrus synchronization protocols in mediterranean italian buffaloes (bubalus bubalis). | an ultrasound assessment of follicle turnover following two different protocols for synchronization of oestrus and ovulation, as well as an assessment of achieved synchronization between ovulation and ai and conception rates in nulliparous and pluriparous buffaloes were carried out during months of increasing day length. nulliparous buffaloes (n = 30) were subjected only to ovsynch protocol whereas pluriparous buffaloes (n = 31) were assigned to ovsynch (n = 14) or to prid-pregnant mare serum go ... | 2005 | 16149950 |
| nucleic acids and protein content in relation to growth and regression of buffalo (bubalus bubalis) corpora lutea. | information on nucleic acids and protein content of buffalo corpus luteum (cl) in relation to growth, development and regression is not available. an experiment was thus conducted to investigate the variation and relationship between nucleic acids and protein content in cl during different developmental stages and to determine the qualitative differences in protein constituents in any of these stages. buffalo corpora lutea of different developmental stages viz., developing (day 5-10, n = 16), de ... | 2006 | 16171957 |
| development of water buffalo (bubalus bubalis) embryos from in vitro matured oocytes reconstructed with fetal skin fibroblast cells as donor nuclei. | the present study was carried out to explore the feasibility of using buffalo fetal skin fibroblasts as donor nuclei and to find out the developmental competence of embryos following transfer of these nuclei to in vitro matured enucleated buffalo oocytes. skin cells were isolated from 1 to 2-month-old fetuses obtained from slaughterhouse, by enzymatic digestion (0.5% w/v trypsin +0.05% w/v collagenase in dulbecco's pbs) for 15-20 min. the cells were washed 4 times with dulbecco's pbs and then on ... | 2006 | 16182475 |
| human ige binding to the glycosidic moiety of bovine kappa-casein. | ige ability for recognizing milk proteins was assayed in the serum of an adult atopic patient who outgrew cow milk allergy in early childhood. a number of protein species included in casein from bovine milk were detected by human ige in immunoblotting experiments. comparing these results with those obtained from an analysis using antibody preparations specifically directed toward the different casein fractions, ige-reactive bands were identified as isoforms of kappa-casein. ige-reactive protein ... | 2005 | 16190658 |
| phylogeny of trypanosoma ( megatrypanum ) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on ssu and its ribosomal sequences. | ssu ribosomal sequences of trypanosomes from brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic t. theileri and allied species parasitic in artiodactyls. t. theileri trypanosomes from distinct geographical regions in brazil and from other countries were tightly clustered into the 'clade t. theileri' distant from the 't. brucei clade' of pathogenic parasites of artiodactyls, and also distinct from trypanosomes of other mammals. the existence of ... | 2006 | 16197590 |
| molecular characterization and prokaryotic expression of buffalo (bubalus bubalis) interleukin-6. | the study describes the characterization of interleukin-6 cdna and essential promoter sequences of the indian water buffalo (bubalus bubalis) and expression of the recombinant il-6 in escherichia coli. buffalo il-6 shows very high nucleotide level identity of the cdna (98.7%) and promoter (98%) sequences with the corresponding cattle sequences. all the major regulatory elements of il-6 promoter like ap-1, multiple response element, nf-il6, ets binding domain and nf-kappab binding sites show abso ... | 2006 | 16199257 |
| changes in uterine protein secretion during luteal and follicular phases and detection of phosphatases during luteal phase of estrous cycle in buffaloes (bubalus bubalis). | changes in uterine proteins during different reproductive states and their functional significance though known in other species have not been established in buffaloes. an attempt has been made to unravel the changes in composition of buffalo uterine secretion with growth and regression of corpora-lutea during early, mid and late luteal and follicular phase of estrous cycle using gel filtration and electrophoresis techniques. also the phosphatases activities in luteal phase uterine secretions ha ... | 2006 | 16213013 |
| binucleate trophoblast giant cells in the water buffalo (bubalus bubalis) placenta. | the binucleate trophoblast giant cells (bnc) of the water buffalo, bubalus bubalis, placenta were studied, with emphasis on the synthesis of bnc-specific proteins. placentomal tissues of 27 water buffalos (2-10 months of pregnancy) were processed for light and electron microscopy. the frequency of bncs was 20% of the trophoblastic cells in 2-3-month placentas and increased to 27% in the later stages. ultrastructurally, binucleate cells displayed a prominent granular endoplasmic reticulum and gol ... | 2006 | 16240388 |
| ear-tag retention and identification methods for extensively managed water buffalo (bubalus bubalis) in trinidad. | thirty-two young domestic water buffalo were studied to evaluate ear-tag retention during an epidemiologic field trial. plastic ear-tags were placed in both ears before the start of the trial, which was implemented in an extensively managed domestic water buffalo herd of approximately 1000 animals in trinidad from 1999-2001. the presence or absence of ear-tags was recorded at the times of animal handling. the rate of ear-tag loss was modeled using a parametric survival analysis assuming an expon ... | 2006 | 16242197 |
| anomalous mrna levels of chromatin remodeling genes in swamp buffalo (bubalus bubalis) cloned embryos. | the swamp buffalo (bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. nuclear transfer (nt) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. however, cloned buffalo embryos have impaired development, as in other species. to understand the chromatin remodeling activities in cloned embryos and to improve nt technology, we examined the expression profiles of five genes invol ... | 2006 | 16256185 |
| high-performance liquid chromatography of governing liquid to detect illegal bovine milk's addition in water buffalo mozzarella: comparison with results from raw milk and cheese matrix. | a method to detect fraudulent addition of bovine milk in water buffalo mozzarella cheese by gradient high-performance liquid chromatography (rp-hplc), relying on the measurement of quantity ratios within beta-lactoglobulin protein family, is described. analyses were performed on raw milk, cheese matrix and cheese governing liquid using a c4 column and uv detection. this work demonstrated that bovine milk addition during cheesemaking can be detected in governing liquid of mozzarella down to the e ... | 2005 | 16257304 |
| comparative fish mapping of mucin 1, transmembrane (muc1) among cattle, river buffalo, sheep and goat chromosomes: comparison between bovine chromosome 3 and human chromosome 1. | four bovine bac clones (0494f01, 0069d07, 0060b06, and 0306a12) containing muc1, as confirmed by mapping muc1 on a rh3000 radiation hybrid panel, were hybridised on r-banded chromosomes of cattle (bta), river buffalo (bbu), sheep (oar) and goat (chi). muc1 was fish-mapped on bta3q13, bbu6q13, oar1p13 and chi3q13 and both chromosomes and chromosome bands were homoeologous confirming the high degree of chromosome homoeologies among bovids and adding more information on the pericentromeric regions ... | 2006 | 16276097 |
| histo-morphology of the uterus and early placenta of the african buffalo (syncerus caffer) and comparative placentome morphology of the african buffalo and cattle (bos taurus). | differences exist in reproductive physiology between african buffalo (syncerus caffer), cattle (bos taurus) and water buffalo (bubalus bubalis). the aim of this study was to histo-morphologically compare the anatomy of non-pregnant and pregnant uteri of buffalo and cattle. two non-pregnant uteri and placentae of six pregnant african buffalo were used. early placentome formation (fetal crown rump length (crl): 2-17.5 cm) in s. caffer and b. taurus was compared. the endometrium of buffalo uteri co ... | 2006 | 16293303 |
| effects of xylazine, lignocaine and their combination for lumber epidural analgesia in water buffalo calves (bubalus bubalis). | the study was conducted to evaluate the effects of xylazine alone (0.05 mg/kg), lignocaine alone (2.0 mg/kg) and a combination of xylazine and lignocaine (0.05 mg/kg and 2.0 mg/kg, respectively) after lumbar epidural administration in water buffalo calves. fifteen nondescript, male water buffalo calves of 6-8 months of age and weighing between 55 and 75 kg were randomly placed in 3 groups (a, b and c). the agents were administered at the 1st lumbar epidural space. clinico-physiological parameter ... | 2005 | 16300183 |
| the occurrence of nymphal stage of linguatula serrata in water buffaloes (bubalus bubalis): nymphal morphometry and lymph node pathology. | the mesenteric lymph nodes (mln) of buffaloes (n = 100) were examined for the presence of parasitic infection. the nymphal stage of linguatula serrata was observed in two buffaloes. a single white-coloured nymph with transversely striated spines on a segmented body, two pairs of oral suckers and hooks was observed in the mln. the morphometrics of the nymphs were studied. the affected lymph nodes were grossly enlarged with cyst and showed pathological lesions of fibroblastic reaction with a mild ... | 2005 | 16300658 |
| the role of wild animal populations in the epidemiology of tuberculosis in domestic animals: how to assess the risk. | tuberculosis is present in wild animal populations in north america, europe, africa and new zealand. some wild animal populations are a source of infection for domestic livestock and humans. an understanding of the potential of each wild animal population as a reservoir of infection for domestic animals is reached by determining the nature of the disease in each wild animal species, the routes of infection for domestic species and the risk of domestic animals encountering an infectious dose. the ... | 2006 | 16326039 |
| occurrence of the rumen ciliate oligoisotricha bubali in domestic cattle (bos taurus). | oligoisotricha bubali, previously observed twice in water buffalo, was detected in rumen contents of domestic cattle (bos taurus) in two different areas of tennessee. concentrations ranged from <1 to 35% of the total protozoa in unweaned calves and up to 72% in older animals in feedlot. in contrast to the other genera of holotrichs, both total numbers and percent composition of o. bubali increased when animals were fed a corn silage-concentrate diet. | 1983 | 16346277 |
| cloning and biological characterization of buffalo (bubalus bubalis) interferon-gamma. | interferon-gamma, a major immunomodulatory cytokine, of indian water buffalo (bubalus bubalis) was characterized at molecular level. complementary dna and essential promoter region were cloned and sequenced, and functional recombinant protein was expressed in bacterial system. the cdna has 97.8% nucleotide identity with 11-nucleotide and four-amino acid variations, and the essential promoter region has 98.4% identity with five-nucleotide variations and a four-nucleotide deletion in comparison wi ... | 2006 | 16360017 |
| transcriptional status of known and novel genes tagged with consensus of 33.15 repeat loci employing minisatellite-associated sequence amplification (masa) and real-time pcr in water buffalo, bubalus bubalis. | we conducted minisatellite-associated sequence amplification (masa) with an oligo (5' cacctctccacctgcc 3') based on consensus of 33.15 repeat loci using cdna from the testis, ovary, spleen, kidney, heart, liver, and lung of water buffalo bubalus bubalis and uncovered 25 amplicons of six different sizes (1,263, 846/847, 602, 576, 487, and 324 base pairs). these fragments, cloned and sequenced, were found to represent several functional, regulatory, and structural genes. blast search of all the 25 ... | 2006 | 16405399 |
| identification of x- and y-chromosome bearing buffalo (bubalus bubalis) sperm. | the aim of this study was to identify the difference in dna content characterizing the x- and y-chromosome bearing sperm of buffalo. sperm from six murrah buffaloes and six nili-ravi buffaloes were collected and stained with hoechst 33342 followed by flow cytometry analysis of the dna content. two symmetrical, separate but overlapping peaks presumed to be x- and y-chromosome bearing sperm were detected. the difference in fluorescence intensity, which related to the dna content, between the x- an ... | 2006 | 16413705 |
| increased frequencies of both chromosome abnormalities and sces in two sheep flocks exposed to high dioxin levels during pasturage. | during the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of naples and caserta (southern italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of fat, as human who 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd or dioxin) equivalent]. while some farms, especially those showing relatively low levels of dioxins, have managed to reduc ... | 2006 | 16434450 |
| [populational alteration of cells in the intestinal intraepithelial layer and morphological changes of the intestinal wall elicited by toxocara vitulorum infection in buffalo calves (bubalus bubalis)]. | to understand the development of the inflammatory responses in the wall of the gut, during the process of expulsion of the parasites from the host, samples of tissues were removed from the small intestines from four groups of naturally infected buffalo calves with toxocara vitulorum during the beginning of the infection, at the peak of egg output, during the period of expulsion and post-expulsion of the worms, as well as from uninfected calves. cells (mast cells, eosinophils, intraepithelial lym ... | 2005 | 16445869 |
| cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (g1) and buffalo (g3) strains. | a survey of cystic echinococcosis (ce) in the water buffalo (bubalus bubalis) of the italian mediterranean breed was carried out in campania, a region of southern italy. in addition, a molecular study was performed on 48 hydatid cysts coming from 48 water buffaloes in order to determine the echinococcus granulosus strain(s) present in this host. out of a total of 722 water buffaloes examined for ce, 76 (10.5%) were found infected. the average number of cysts per buffalo was 4.3 (minimum 1, maxim ... | 2006 | 16480832 |
| genetic resistance to brucella abortus in the water buffalo (bubalus bubalis). | brucellosis is a costly disease of water buffaloes (bubalus bubalis). latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. we exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. we tested 231 water buffalo cows for the presence of anti-brucella abortus antibodies (by the agglutination and complement fixation tests) and the nramp1 genotype (by pcr-denaturin ... | 2006 | 16552040 |
| hematological and biochemical changes in water buffalo calves (bubalus bubalis) infected with trypanosoma evansi. | four water buffalo calves (bubalus bubalis) were each inoculated intravenously with 10(6)t. evansi (camel isolate) and the fifth calf kept as non-infected control. the blood and sera of all calves were examined every 4 days during the first month post-inoculation (pi) and then once weekly until the end of the experiment (88 days pi). they were examined for hematological and biochemical changes, liver and kidney function tests. hemoglobin concentration (hb%), packed cell volume (pcv) and red bloo ... | 2006 | 16567045 |
| ultrastructural and morphometric characterization of buffalo (bubalus bubalis) ovarian preantral follicles. | the main objective of the present study was to characterize buffalo preantral ovarian follicles. parts of ovarian cortex, collected from postpubertal buffalo females that were having estrous cycles at regular intervals, were selected under stereomicroscopy and processed for optic and transmission electron microscopy. primordial follicles were characterized as an oocyte encircled by one layer of flattened cells. the buffalo primordial follicle has a mean diameter of 35 microm and the oocyte diame ... | 2007 | 16580155 |
| likelihood ratio estimation without a gold standard: a case study evaluating a brucellosis c-elisa in cattle and water buffalo of trinidad. | the likelihood ratio (lr) is a measure of association that quantifies how many more times likely a particular test result is from an infected animal compared to one that is uninfected. they are ratios of conditional probabilities and cannot be interpreted at the individual animal level without information concerning pretest probabilities. their usefulness is that they can be used to update the prior belief that the individual has the outcome of interest through a modification of bayes' theorem. ... | 2006 | 16600408 |
| effect of bradykinin on murrah buffalo (bubalus bubalis) semen cryopreservation. | keeping in view the poor freezability of bubaline semen in conventionally used extenders, this study was conducted on three murrah bulls to improve semen cryopreservation with the incorporation of bradykinin (0.5, 1.0 and 2.0 ng ml(-1)) in routinely used egg yolk tris-glycerol (eytg) extender. bradykinin (2.0 ng ml(-1)) had significant (p<0.05) beneficial effect on live sperm % (81.6+/-1.8) and hypo osmotic swelling (hos) % (63.0+/-1.3) as compared to their respective control values of 73.4+/-2. ... | 2007 | 16600533 |
| interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |
| chromosomal localization, copy number assessment, and transcriptional status of bamhi repeat fractions in water buffalo bubalus bubalis. | higher eukaryotes contain a wide variety of repetitive dna, although their functions often remain unknown. we describe cloning, chromosomal localization, copy number assessment, and transcriptional status of 1378- and 673-bp repeat fractions in the buffalo genome. the pds5, representing the 1378-bp fragment, showed fish signals in the centromeric region of acrocentric chromosomes only, whereas pds4, corresponding to 673 bp, detected signals in the centromeric regions of all the chromosomes. cros ... | 2006 | 16629593 |
| subcutaneous pharmacokinetics and dosage regimen of cefotaxime in buffalo calves (bubalus bubalis). | the pharmacokinetics and dosage regimen of cefotaxime following its single subcutaneous administration (10 mg/kg) were investigated in buffalo calves. plasma and urine samples were collected over 10 and 24 h post administration, respectively. cefotaxime in plasma and urine was estimated by microbiological assay technique using e. coli as test organism. the pharmacokinetic profiles fitted one-compartment open model. the peak plasma levels of cefotaxime were 6.48 +/- 0.52 microgram/ml at 30 min an ... | 2006 | 16645334 |
| rapid sexing of water buffalo (bubalus bubalis) embryos using loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel dna amplification method that amplifies a target sequence specifically under isothermal conditions. the objective of this study was to identify a y chromosome-specific sequence in water buffalo and to establish an efficient procedure for embryo sexing by lamp. the homologues of a y chromosome-specific sequence, bovine repeat y-associated.2, in swamp and river buffalo were cloned, and designated swamp buffalo repeat y-associated.2 and river ... | 2006 | 16672158 |
| cryopreservation of in vitro matured buffalo (bubalus bubalis) oocytes by minimum volumes vitrification methods. | the aim of this study was to evaluate the efficiency of the solid surface vitrification (ssv) and the cryoloop vitrification (clv) methods to cryopreserve in vitro matured buffalo oocytes. another objective of the work was to investigate whether the presence of cumulus cells affects the efficiency of oocyte vitrification in this species. in the ssv method, oocytes were vitrified in a solution of 35% ethylene glycol, 5% polyvinyl-pyrrolidone and 0.4% trehalose and they were warmed in a 0.3m treha ... | 2007 | 16698202 |
| oestrus control and early pregnancy diagnosis in the swamp buffalo: comparison of enzymeimmunoassay and radioummunoassay for plasma progesterone. | a preliminary study has been undertaken, in order to investigate the suitability of a progesterone assay in blood plasma for oestrus control and pregnancy diagnosis in the swamp buffalo (bubalus bubalis ). progesterone was determined both by radioimmunoassay (ria) and by enzymeimmunoassay (eia). values obtained by eia were considerably lower than values obtained by ria. this may be partly due to the fact that only ria values were corrected for procedural losses. blood samples were taken on day 1 ... | 1979 | 16725424 |
| effect of prostaglandin f(2alpha)- thamsalt and estrumate (ici 80996) on plasma progesterone levels in indian water buffaloes (bubalus bubalis ). | thirty six buffalo showing normal reproductive cyclas were given a single intramuscular injection of prostaglandin f(2alpha)- thamsalt (pgf(2alpha)) and estrumate on day 9 or 15 of the estrous cycle in order to induce estrus. in pgf(2alpha) treated animals, plasma progesterone dropped from 2.99+/-0.29 to 0.10 +/- 0.02 ng/ml and from 5.53 +/- 0.53 to 0.09 +/- 0.03 ng/ml (mean +/- sem) within 72 hours of treatment, on day 9 and 15, respectively. similarly, the treatment with estrumate on day 9 or ... | 1980 | 16725497 |
| pregnancy in suboestrus buffaloes (bubalus bubalis) after treatment with prostaglandin f(2) alpha. | seventy six apparently anoestrous buffaloes, with a palpable corpus luteum at day 43 to 547 post partum, were treated intramuscularly with prostaglandin, using either 25 mg single dose (group i), 25 mg double dose 11 days apart (group ii), or 50 mg single dose (group iii). animals exhibiting oestrus, without any treatment, during the period of experimentation served as control (group iv). the overall conception rate for the treated animals was 28.8, 51,5, and 69.7 percents after one, two, and th ... | 1981 | 16725572 |
| peripartal changes of estrone, progesterone and prostaglandin in the water buffalo. | the peripheral blood plasma concentration of estrone, progesterone and 15-keto-13, 14-dihydroprostaglandin f2alpha (pgf2alpha metabolite) were determined by radioimmunoassay techniques during the peripartal period in 5 buffalo cows belonging to a river type breed. estrone levels started to increase from below 200 pg/ml about 15 days prior to parturition, and reached high concentrations (400-750 pg/ml) during the last 5 days of pregnancy. the estrone concentration decreased to baseline levels aft ... | 1981 | 16725607 |
| serum fsh levels during estrus and a 4-week period following mating in murrah buffaloes (bubalus bubalis ). | circulating fsh levels of 13 murrah buffaloes were investigated by a double-antibody radioimmunoassay using a homologous bovine system. the mean (+/- s.e.m.) peak fsh level on the day of estrus was if57.94 +/- 12.84 ng/ml of blood serum during hotter months (june-august) and 65.23+/-10.54 ng/ml during cooler months (october-december). these were much higher than the fsh concentrations recorded during the rest of the cycle post-mating. there were sizable fluctuations in fsh levels on different da ... | 1982 | 16725678 |
| serum prolactin levels of non-cycling murrah buffaloes (bubalus bubalis ). | prolactin levels were quantified by double antibody radio-immunoassay in the blood sampled daily for 24 days, from 9 non-cyclic murrah buffaloes during hot months (ambient temp.41 to 43 degrees c). all buffaloes were kept in open loose housing. three buffaloes were sprinkled with water for half an hour twice daily between 1200 and 1430 h to overcome partly the effect of heat during the period of investigation. mean prolactin levels of 6 unsprinkled buffaloes ranged on different days from 249 to ... | 1982 | 16725711 |
| clinical analysis of reproductive failure among female buffaloes (bubalus bubalis) under village management in andhra pradesh. | analysis of the clinical data of 20, 439 infertile graded murrah female buffaloes (bubalus bubalis ) of small and marginal farmers in andhra pradesh, examined over a period of two years, revealed that 2.50, 73.77 and 23.73 percent of the animals had reproductive disorders which were of anatomical, functional and non-specific infectious nature, respectively. highest frequencies were of infantile tracts, ovarian quiescence and endometritis in each group, which together constituted 79.13 percent. t ... | 1982 | 16725762 |
| bio-histopathological studies of endometrium in repeat breeding buffaloes (bubalus bubalis). | a total of 24 uterine biopsies were successfully obtained from 40 repeat-breeding buffaloes. the endometrium of 15 buffaloes showed marked neutrophilic infiltration in the epithelium, stroma and uterine glands. in some cases, lymphocyte and plasma cell infiltration, cystic dilatation of the glands and periglandular fibrosis indicated chronicity of the endometritis. most of the animals (85.0%) had uterine cultures positive for streptococci, staphylococci, gram positive variable cocci, gram negati ... | 1983 | 16725782 |
| luteinizing hormone in blood plasma of post-partum buffaloes (bubalus bubalis). | the changes in luteinizing hormone concentration were measured by heterologous double-antibody radioimmunoassay in blood plasma of 28 murrah buffaloes. the lh concentration fluctuated between 0.22 to 0.78 ng/ml during first 21 days post-partum. the level increased significantly (p < 0.001) at estrus. the basal lh concentration of second and third week post-partum was inversely related to the first post-partum ovulation interval. | 1983 | 16725786 |
| total lipids and phospholipids in buffalo semen (bubalus bubalis). | spermatozoal and seminal plasma concentrations of total lipids from 50 ejaculates and phospholipids and their fractions from 30 ejaculates were quantified in the semen of five murrah buffalo bulls. sperm lipid content ranged from 0.93 to 1.72 mg/10(9) cells with an overall average 1.32+/-0.03 mg/10(9) cells. its concentration in seminal plasma varied from 1.39 to 2.22 mg/ml with overall average of 1.75+/-0.03 mg/ml. spermatozoal total phospholipid content ranged from 0.44 to 0.94 mg/10(9) cells ... | 1983 | 16725867 |
| embryo transfer in water buffalo (bubalus bubalis). | a normal, live 35-kg water buffalo bull calf was born 300 days after it was nonsurgically collected as a 7-day blastocyst from a water buffalo donor and transferred nonsurgically to an unrelated water buffalo recipient. the development of estrus synchronization, superovulation and estrus detection methods in water buffalo are described. | 1983 | 16725875 |
| relationship of scrotal circumference and testicular volume to age and body weight in the swamp buffalo (bubalus bubalis). | scrotal circumference (sc) and testicular volume (tv) were measured in 65 swamp buffalo bulls ranging in age from 7 to 60 months and weighing 130 kg to 560 kg. ages and body weight (bw) were recorded for each male at the time of measurement to find out if they correlated with sc and tv. sc and tv increased linearly and correlated significantly with age and bw (sc vs age: r=0.74, p<0.01; sc vs bw: r=0.88, p<0.01; tv vs bw: r=0.82, p<0.01). sc measurements ranged from 15.1+/-1.1 cm to 24.0+/-0.4 c ... | 1984 | 16725943 |
| induction of parturition in water buffaloes (bubalus bubalis ). | parturition was induced in ten buffaloes by combining treatments of dexamethasone and vetoestrol, so that they calved about one month before the expected term. either dexamethasone alone (group b) or dexamethasone and vetoestrol (group c) was used. another five animals served as controls (group a). calving was induced in four animals in group b and three animals in group c after two injections of the compounds four days apart. the average time from first injection to calving for these animals wa ... | 1985 | 16726001 |
| dystocia due to urinary bladder carcinoma in two water buffaloes (bubalus bubalis) - clinical case report. | two buffaloes with full-term pregnancy suffered from dystocia because the cervix did not dilate in spite of strong labour pains and other parturition signs shown by each animal. the urinary bladder, cervix, vagina and surrounding area were very firm. dead, emphysemated fetuses were removed by caesarean in each case and anuria was also noticed. one buffalo died and the other was euthanised after surgery because it did not improve. the post-mortem examinations revealed transitional cell carcinoma ... | 1985 | 16726085 |
| comparative studies on the superovulatory effect of pmsg and fsh in water buffalo (bubalus bubalis ). | a total of thirty-eight lactating water buffalo cows were treated in four experiments simultaneously either with fsh (first group) or pmsg(second group). to the first group (half of the animals), a total dose of 40 mg fsh-p at 12-hr intervals was given i.m. within a 4-day period. the second group was treated i.m. with 3000 iu pmsg (gestyl). forty-eight hours after initiation of the superovulatory treatment all buffaloes were given 500 ug cloprostenol. fi seen buffaloes from the fsh-treated group ... | 1986 | 16726169 |
| studies on serum progesterone levels in relation to occurrence of uterine torsion in buffaloes (bubalus bubalis ). | serum samples from 32 buffaloes suffering from uterine torsion at approximately the completion of the gestation period were analyzed for progesterone concentrations by radioimmunoassay. fifteen buffaloes had progesterone concentrations of more than 1.0 ng/ml (1.65 +/- 0.13 ng/ml; 1.1 to 2.8 ng/ml), while the serum progesterone concentrations of the remaining 17 buffaloes were below 1.0 ng/ml (599 +/- 59 pg/ml; 200 to 900 pg/ml). the occurrence of uterine torsion was associated with labour and/or ... | 1986 | 16726204 |
| treatment of summer anestrous buffalo (bubalus bubalis) with progesterone releasing intravaginal device plus pregnant mare serum go nadotropin. | three experiments were conducted to treat forty-two summer-anestrous buffalo at three different villages. in all the experiments animals were randomly divided into two groups. one group was treated with a progesterone releasing intravaginal device (prid) plus 800 iu pregnant mare serum gonadotropin (pmsg) at the time of prid removal. the second group was retained as untreated control animals. all the treated animals were clinically examined and inseminated twice 48 and 72 h post treatment. ovula ... | 1988 | 16726443 |
| successful nonsurgical embryo transfer in buffalo (bubalus bubalis ) in bulgaria. | forty-one day 5.0 to day 5.5 embryos and one unfertilized ovum were recovered nonsurgically from 24 superovulated, parous buffalo (bubalus bubalis ) and transferred nonsurgically to 28 synchronized recipients by a team of bulgarian and american scientists. five pregnancies were established and four live buffalo calves were born at the end of normal gestation periods. | 1988 | 16726508 |
| sialo proteins and phosphatases in estrual cervical mucus of fertile and repeat breeder buffaloes (bubalus bubalis ). | concentrations of sialic acid and protein and activities of acid phosphatase (acp) and alkaline phosphatase (akp) were determined in estrual cervical mucus of fertile and repeat breeder buffaloes during late estrus. significantly (p < 0.01) higher concentrations of sialic acid and acp and akp activities were observed in fertile buffaloes as compared with repeat breeder buffaloes. however, differences were nonsignificant in protein content between fertile and repeat breeder buffaloes, albeit prot ... | 1988 | 16726525 |
| the effects of capture stress on testis function in the australian swamp buffalo (bubalus bubalis ). | capture stress significantly affected the cellular composition and function of the testis and epididymis of feral swamp buffalo bulls. there was an initial acute drop in the population of elongated spermatids, round spermatids and late primary spermatocytes, and subsequently, a more gradual decline in the early elongated spermatid and late primary spermatocyte populations as the duration of the stress increased. sertoli cell numbers were unaffected. the most marked effect was on the early elonga ... | 1989 | 16726578 |
| early embryonic development in thai swamp buffalo (bubalus bubalis ). | a total of 33 nonsurgical embryo collections was carried out to investigate early embryo development in thai swamp buffalo. collections were performed on days 5.5, 6.0, 6.5, 7.0 and 7.5. the different stages of embryo development on these days were the 16-cell stage, compact morula, blastocyst, hatched blastocyst and hatched expanding blastocyst, respectively. in addition, some degenerating embryos and unfertilized ova were also recovered. a higher recovery rate was obtained with single embryo c ... | 1989 | 16726630 |
| superovulatory response of river bufalo (bubalus bubalis ). | the ovarian response of 25 buffalo-cows was visually assessed, and their oviducts and uteri separately flushed 3 to 6 d post superovulatory estrus at slaughter. ten buffalo-cows slaughtered on days 5 and 6 were examined per rectum for corpora lutea (cl) and follicles > 8 mm prior to slaughter, and the estimate was compared later with the actual ovarian response. five out of the ten buffalo-cows were nonsurgically flushed in vivo on day 5 of the estrous cycle, a day before slaughtering, and as a ... | 1990 | 16726742 |
| effect of uterine fluid on in vitro acrosome reaction of buffalo (bubalus bubalis ) spermatozoa. | the present study was undertaken to examine in vitro acrosome reaction in the uterine fluid of estrous buffalo. successful acrosome reaction was achieved by incubating buffalo spermatozoa in 2% detoxified uterine fluid in biggers whitten whittingham (bww) medium, ph 7.4 at 37 degrees c and a sperm concentration of 36 x 10/ml. neat uterine fluid has been found to be toxic to spermatozoa, hence we detoxified the uterine fluid at 56 degrees c for 30 min, which resulted in higher percentage of sperm ... | 1990 | 16726752 |
| monosomy x and gonadal dysgenesis in a buffalo heifer (bubalus bubalis). | investigations on a 3-yr-old river buffalo heifer presenting anestrus revealed a chromosome make-up of 2n=49 in the lymphocyte cultures, compared with the 2n=50 characteristic of riverine buffalo. the missing chromosome was identified as one of the xs by karyotypic analysis, and monosomy x was confirmed by c and g-banding techniques. both ovaries of the heifer were underdeveloped, although the other components of the internal genitalia were normal. the phenotypic and karyotypic features confirme ... | 1990 | 16726819 |
| endocrinological evaluation of the induction of superovulation with pmsg in water buffalo (bubalus bubalis). | ten nonlactating buffalo were superovulated with 3000 iu pmsg. luteolysis was induced with 500 microg cloprostenol (pg) 60 and 72 h after pmsg. five buffalo were alloted for natural mating and five were bred by artificial insemination 60 and 84 h after the first pg treatment. since four buffalo developed pyometra, only 6 of 10 underwent embryo collection successfully 180 to 190 h after pg. three buffalo yielded only one morula each, while the remaining three yielded a total of two, three and fou ... | 1990 | 16726846 |
| the use of the probit model to predict pregnancy status of buffalo based on physio-chemical properties of estrual mucus. | improper timing of artificial insemination with respect to ovulation is one of the major factors hampering the conception rate in buffalo. the present study was an attempt to relate physio-chemical changes in estrual mucus to subsequent pregnancy status in order to find their optimal values for determining the time for artificial insemination (ai). serum estradiol, total protein and dry matter contents of estrual mucus were evaluated to predict the subsequent pregnancy in 36 buffalo during octob ... | 1991 | 16726984 |
| in vitro fertilization of water buffalo follicular oocytes and their ability to cleave in vitro. | water buffalo (murrah) oocytes were collected from ovaries obtained from a local slaughterhouse. they were classified according to the character of the cumulus cells under a stereomicroscope and then cultured in 25 mm hepes buffered tissue culture medium-199 (tcm-199) supplemented with 5% estrous water buffalo serum in an atmosphere containing 5% co2 in air at 39 degrees c. after 20 to 24 hours of in vitro maturation, the oocytes were cultured at 38.5 degrees c in tcm-199 supplemented with 1% es ... | 1992 | 16727214 |
| effects of bull and heparin and sperm concentrations on in vitro fertilization of buffalo (bubalus bubalis ) oocytes matured in vitro. | a study was undertaken to assess the ability of spermatozoa from 6 buffalo bulls, at different levels of heparin and sperm concentrations, to achieve an acceptable level of fertilization in vitro. frozen-thawed spermatozoa, 3 dosages of heparin (0, 10 and 100 ug/ml) in the presence and absence of penicillamine, hypotaurine and epinephrine (phe), and 4 sperm concentrations (1 x 10(6), 2 x 10(6), 3 x 10(6) and 4 x 10(6) /ml) were studied using 3202 buffalo oocytes. the mean proportions of fertiliz ... | 1993 | 16727261 |
| in vitro maturation and fertilization of buffalo oocytes (bubalus bubalis ): effects of media, hormones and sera. | media (tcm-199 and ham's f-10); sera (fetal calf serum, fcs, and buffalo estrous serum, bes); and hormones (fsh, 0.5 ug/ml, lh, 5 ug/ml and estradiol 1 ug/ml) were tested to determine the efficiency of in vitro maturation and fertilization of buffalo follicular oocytes. immature good quality cumulus-oocyte complexes (cocs) were randomly assigned to 1 of 4 experiments. each experiment consisted of 6 treatment groups. oocytes cultured for 24 hours in medium (tcm-199 or ham's f-10) containing 10% f ... | 1993 | 16727284 |
| a simplified laparoscopy technique for repeated ovarian observation in the water buffalo (bubalus bubalis). | a simplified technique of laparoscopy was developed for ovarian observation in the riverine buffalo, through a right paralumbar incision. the technique differed from previously described ones in that it involved only a single puncture and required no abdominal insufflation. a hopkins 0 degrees forward viewing endoscope (5.5 mm x 500 mm) in combination with an endoscope sheath having a built-in instrument channel, and a long flexible forceps (630 mm) were used. of the 23 observation attempts on 1 ... | 1993 | 16727332 |
| ultrasonographic imaging to monitor early pregnancy and embryonic development in the buffalo (bubalus bubalis). | real time b-mode ultrasound was used to detect and monitor the early conceptus, its growth and its anatomical features in 26 buffalo between days 18 and 62 of gestation. the buffalo were artificially inseminated, and the conceptuses were examined on alternate days beginning on day 18. the embryonic vesicle and the embryo proper within the vesicle was first visible in 12 of the buffalo on mean day (+/-sd) 19.00+/-2.1 and day 19.0+/-1.69, respectively. the heartbeat of the embryo proper could be d ... | 1994 | 16727424 |
| a new surgical approach to internal genitalia in water buffalo (bubalus bubalis): a preliminary study. | new surgical access to internal genitalia through either the left or right lateral wall of the pelvic cavity was sought in 3 multiparous buffalo cows. (murrahxmediterranean buffalo crossbreed). an incision 12 to 13 cm long was made in the dorso-caudal part of the regio glutea, equidistant from the tuber ischiadicum and the most prominent point of the crista sacralis mediana (in the space confined dorsally by the caudal part of os sacrum and first 2 to 3 vertebrae coccygeae and ventrally by the s ... | 1994 | 16727431 |
| chromosome analysis of murrah buffalo (bubalus bubalis ) spermatozoa using zona-free hamster oocytes. | an interspectific in vitro fertilization system was adopted to analyse sperm chromosomes of murrah buffalo (bubalus bubalis ). superovulation was induced in mature female golden hamsters (mesocricetus auratus ) to obtain a large number of oocytes. the zona pellucidae were digested by trypsin treatment. zona free hamster oocytes were penetrated by buffalo spermatozoa capacitated with calcium ionophore a23187. fertilized ova were cultured in tc 199 medium supplemented with 10% fetal calf serum (fc ... | 1994 | 16727537 |