| [subcellular mimical localization of the atp synthase b subunit from clonorchis sinensis under different conditions of cell cycling]. | to investigate the subcellular localization of atp synthase b subunit from clonorchis sinensis under different conditions of hela cell cycling, and the effect of this protein on the expression of its encoding-gene and homologous host genes. | 2010 | 21351540 |
| identification and functional characterization of csstefin-1, a cysteine protease inhibitor of clonorchis sinensis. | cathepsin fs of clonorchis sinensis (cscfs) are major secreted proteins that are expressed in the intestine of the parasite and play pivotal roles in parasite nutrition and host-parasite interactions. however, strict regulation of their activities is also essential to minimize inadequate superfluous damage to the parasite and host. in this study, we identified and characterized a novel cysteine protease inhibitor of c. sinensis, csstefin-1, as a modulator of cscfs. csstefin-1 was shown to be a t ... | 2011 | 21354219 |
| molecular cloning, expression, and characterization of cyclophilin a from clonorchis sinensis. | this study described the recognization, cloning, and recombinant expression of cyclophilin a-like gene from clonorchis sinensis adult complementary dna library (cscypa) and its expression and secretion in adult. western blotting demonstrated the recombinant cscypa could be recognized by sera of clonorchiasis patients and a sole protein of the same size in the excretory-secretory antigens of in vitro cultured adult could be recognized by antiserum raised against the recombinant cscypa. immunohist ... | 2011 | 21360097 |
| clonorchis sinensis enolase: identification and biochemical characterization of a glycolytic enzyme from excretory/secretory products. | enolase plays a key role in energy metabolism and development of most organisms. we isolated a gene encoding enolase from clonorchis sinensis (c. sinensis) adult cdna library and expressed the recombinant protein in escherichia coli. c. sinensis enolase (csenolase) was identified as both an excretory/secretory product and a tegumental component of c. sinensis by western blot analysis. the transcriptional level of csenolase was examined at adult worm, metacercaria, cercaria and egg of c. sinensis ... | 2011 | 21382423 |
| molecular cloning, expression, and immunolocalization of the nad(+)-dependent glycerol 3-phosphate dehydrogenase (gpd) from clonorchis sinensis. | glycerol 3-phosphate dehydrogenase (gpd) plays an important role in the energy metabolism and nutrition metabolism. in order to know about the biological functions of gpd of clonorchis sinensis (c. sinensis), we identified a complete gene coding gpd from c. sinensis metacercaria cdna library. this novel cdna sequence contains 1,056 bp with a putative open reading frame of 351 amino acids and shares 74% identity with gpd from schistosoma mansoni. recombinant csgpd was expressed and purified from ... | 2011 | 21409441 |
| proteomic analysis of excretory secretory products from clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory-secretory antigen-fructose-1,6-bisphosphatase. | clonorchis sinensis is a food-borne zoonotic parasite that resides in bile ducts and causes clonorchiasis, which may result in cholelithiasis, cholecystitis, hepatic fibrosis, and liver tumors. although total excretory secretory products (esp) of c. sinensis adults induce hepatic fibrosis in vivo in rats, the causative mechanism is not well understood. to study components of the esp, c. sinensis culture medium was collected and analyzed using shotgun lc-ms/ms. we identified a total of 110 protei ... | 2011 | 21424807 |
| clonorchis sinensis-derived total protein attenuates airway inflammation in murine asthma model by inducing regulatory t cells and modulating dendritic cell functions. | asthma is characterized by th2-mediated inflammation, resulting in airway hyperresponsiveness (ahr) through airway remodeling. recent epidemiological and experimental reports have suggested an inverse relationship between the development of allergy and helminth infections. infection by clonorchis sinensis, a liver fluke that resides in the bile duct of humans, is endemic predominantly in asia including korea and china. using a murine model for asthma, we investigated the effects of c. sinensis-d ... | 2011 | 21440530 |
| [eosinophilia in a patient from thailand-laos.] | | 2011 | 21458890 |
| influencing factors for cure of clonorchiasis by praziquantel therapy: infection burden and cyp3a5 gene polymorphism. | chemotherapy of clonorchiasis with praziquantel (pzq) is effective but about 15% of treated cases have been reported uncured. the present study investigated correlation of single nucleotide polymorphisms (snps) of the cytochrome p450 gene, cyp3a5 and cure of clonorchiasis. a total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg pzq. reexamination recognized 33 (9.5%) uncured and 313 cured. numbers of eggs per gram of feces (epgs) before treatment were significantly ... | 2011 | 21461268 |
| clonorchis sinensis infection is positively associated with atopy in endemic area. | epidemiologic studies have suggested that helminth infections play a protective role against allergy; this inverse association, however, has not been consistent. clonorchis sinensis, the liver fluke of human, is prevalent in the far east. the association between c. sinensis infection and allergy has not yet been reported. | 2011 | 21488996 |
| [cloning, expression of ppmp antigen genes of clonorchis sinensis and immunogenic identification of the recombinant proteins]. | to screen and identify new specific antigen gene from a cdna library of adult clonorchis sinensis, and investigate the immunogenicity of the recombinant proteins. | 2010 | 21500524 |
| development of a double antibody sandwich elisa assay for the diagnosis of angiostrongyliasis. | abstract abstractusing two monoclonal antibodies (mabs) against excretory/secretory (es) antigens of angiostrongylus cantonensis adult worms, a method of double antibody sandwich elisa for the detection of circulating antigens (cag) was established with the best experimental conditions regulated for performance. to evaluate the sensitivity, the cag in sera of rats (80) and mice (15) infected with a. cantonensis as well as cag in sera of clinical confirmed angiostrongyliasis patients (70) were de ... | 2011 | 21506824 |
| rna interference targeting cathepsin b of the carcinogenic liver fluke, opisthorchis viverrini. | functional genomics have not been reported for opisthorchis viverrini or the related fish-borne fluke, clonorchis sinensis. here we describe the introduction by square wave electroporation of cy3-labeled small rna into adult o. viverrini worms. adult flukes were subjected to square wave electroporation employing a single pulse for 20ms of 125v in the presence of 50μg/ml of cy3-sirna. the parasites tolerated this manipulation and, at 24 and 48h after electroporation, fluorescence from the cy3-sir ... | 2011 | 21565281 |
| risk factors for gallbladder cancer and cholangiocarcinoma: similarities, differences and updates. | introduction: far-ranging variation in the incidence of gallbladder cancer (gbc) and cholangiocarcinoma (cca) in different geographic regions on the globe may reflect the risk factor distribution for these tumors methods: the authors give a comprehensive review on the known risk factors for gbc and cca, and analyze both similarities and differences between the risk factors for the two main types of biliary cancer discussion and conclusion: leading risk factors for gbc include gallstones, female ... | 2011 | 21597894 |
| liver flukes: the malady neglected. | liver fluke disease is a chronic parasitic inflammatory disease of the bile ducts. infection occurs through ingestion of fluke-infested, fresh-water raw fish. the most well-known species that cause human infection are clonorchis sinensis, opisthorchis viverrini and opisthorchis felineus. adult flukes settle in the small intrahepatic bile ducts and then they live there for 20-30 years. the long-lived flukes cause long-lasting chronic inflammation of the bile ducts and this produces epithelial hyp ... | 2011 | 21603286 |
| sequences and gene organization of the mitochondrial genomes of the liver flukes opisthorchis viverrini and clonorchis sinensis (trematoda). | opisthorchis viverrini and clonorchis sinensis are important trematodes infecting humans and animals, belonging to the family opisthorchiidae. in the present study, we sequenced the nearly complete mitochondrial (mt) dna (mtdna) sequences of o. viverrini from laos, obtained the complete mtdna sequences of c. sinensis from china and korea, and revealed their gene annotations and genome organizations. the mtdna sequences of o. viverrini, c. sinensis (china isolate), c. sinensis (korea isolate) wer ... | 2011 | 21626421 |
| clonorchis sinensis a new report in egyptian employees returning back from saudi arabia. | clonorchis sinensis, or chinese liver fluke, is a parasite of the fish-eating mammals endemic in the east asia, far east, particularly in china, japan, taiwan, vietnam, and korea and among immigrant refugees to european union and eastern mediterranean countries. this paper reported infection among egyptian family who practice consumption of imported fishes from the far east. diagnosed was based on detection of its characteristic eggs, positive specific elisa, radiological and family behavior and ... | 2011 | 21634255 |
| molecular identification of clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple ligation-depended probe amplification (mlpa). | abstract: background: infections with the opisthorchid liver flukes clonorchis sinensis, opisthorchis viverrini, and o. felineus cause severe health problems globally, particularly in southeast asia. early identification of the infection is essential to provide timely and appropriate chemotherapy to patients. results: in this study we evaluate a pcr-based molecular identification method, multiplex ligation-dependent probe amplification (mlpa), which allows rapid and specific detection of single ... | 2011 | 21649899 |
| comparative cytogenetics of opisthorchid species (trematoda, opisthorchiidae). | in the present study karyotypes and chromosomes of five species of the family opisthorchiidae (opisthorchis felineus (rivolta, 1884), o. viverrini (poirier, 1886), metorchis xanthosomus (creplin, 1846), m. bilis (braun, 1893), and clonorchis sinensis (cobbold, 1875)) were compared. karyotypes of o. felineus, m. xanthosomus, m. bilis and c. sinensis consist of two pairs of large meta- and submetacentrics and five pairs of small chromosomes (2n=14). the karyotype of o. viverrini is 2n=12, which in ... | 2011 | 21798365 |
| molecular characterization of cathepsin b from clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis. | abstract: background: cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. the purpose of this study was to identify a cathepsin b of clonorchis sinensis (cscb) and to investigate its diagnostic value for human helminthiasis. results: the predicted amino acid sequence of the cathepsin b of c. sinensis shared 63%, 52%, 50% identity with that of ... | 2011 | 21794140 |
| distribution of repetitive dna sequences in chromosomes of five opisthorchid species (trematoda, opisthorchiidae). | genomes of opisthorchid species are characterized by small size, suggesting a reduced amount of repetitive dna in their genomes. distribution of repetitive dna sequences in the chromosomes of five species of the family opisthorchiidae (opisthorchis felineus 2n=14 (rivolta, 1884), opisthorchis viverrini 2n=12 (poirier, 1886), metorchis xanthosomus 2n=14 (creplin, 1846), metorchis bilis 2n=14 (braun, 1890), clonorchis sinensis 2n=14 (cobbold, 1875)) was studied with c- and agnor-banding, generatio ... | 2011 | 21791251 |
| cloning, expression, and characterization of a novel opisthorchis viverrini calcium-binding ef-hand protein. | a novel 22.8kda of opisthorchis viverrini (ov) calcium-binding ef-hand protein (ov cabp) was identified and isolated from an immunoscreening of the adult stage ov cdna library by using a human cholangiocarcinoma (cca) serum. this protein was related to other calcium-binding proteins and conserved among the trematodes. ov cabp shared 98% amino acid identity to 22.8kda of clonorchis sinensis cabp and both were classified as a new group of cabp ef-hand protein by multiple sequence alignment and phy ... | 2011 | 21782972 |
| a specific pcr assay for the diagnosis of clonorchis sinensis infection in humans, cats and fishes. | clonorchiasis caused by clonorchis sinensis is a fish-borne parasitic disease which is endemic in a number of countries. using the sequences of the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna) of c. sinensis as genetic markers, a pair of c. sinensis-specific primers was designed and used to establish a specific pcr assay for the diagnosis of c. sinensis infection in humans, cats and fish. this approach allowed the specific identification of c. sinensis after opt ... | 2011 | 21777693 |
| identification and biochemical characterization of two novel peroxiredoxins in a liver fluke, clonorchis sinensis. | summarywe identified 2 novel genes encoding different 2-cys peroxiredoxins (prxs), designated csprx2 and csprx3, in clonorchis sinensis, which invades the human hepatobiliary tracts. the csprx2 gene expression was temporally increased along with the parasite's development and its protein product was detected in almost all parts of adult worms including subtegument, as well as excretory-secretory products. conversely, csprx3 expression was temporally maintained at a basal level and largely restri ... | 2011 | 21777506 |
| strain variation in the susceptibility and immune response to clonorchis sinensis infection in mice. | mice have shown various susceptibility to infection by clonorchis sinensis. to compare the intra-specific variation in the host-parasite relationship of c. sinensis, 6 strains of mice (icr, balb/c, c57bl/6, ddy, cba/n, and c3h/hen) with 3 different haplotypes were evaluated on their susceptibility. the worm recovery rate and immunological responses were observed after 4 and 8weeks of infection with 30 metacercariae. the highest worm recovery rate was observed as 20.7% in the c3h/hen strain after ... | 2011 | 21763454 |
| adult opisthorchis viverrini flukes in humans, takeo, cambodia. | to the editor: opisthorchis viverrini and clonorchis sinensis, the 2 major species of small liver flukes (family opisthorchiidae), cause chronic inflammation in the bile duct, which leads to cholangitis and cirrhosis of the liver, and are a predisposing factor for cholangiocarcinoma (1). human infections with o. viverrini flukes are found along riverside areas of indochina (thailand, lao people's democratic republic [pdr], and vietnam) (2). | 2011 | 21762595 |
| clonorchiasis-a historical review of contributions of japanese parasitologists. | in the early stage of research on clonorchis and clonorchiasis, japanese parasitologists made a tremendous contribution on the elucidation of the life cycle of this parasite and on the epidemiology of the disease. harujiro kobayashi first identified cyprinoid fish as the second intermediate hosts for clonorchis sinensis in 1912, subsequently parafossarulus snails were identified as the first intermediate host by masatomo muto in 1918. kenso ishisaka recorded the first human case of clonorchiasis ... | 2011 | 21749930 |
| clonorchis sinensis and clonorchiasis, an update. | clonorchis sinensis is the most common human liver fluke in east asia. several studies proved its carcinogenesis in humans and it was reclassified as a group 1 biological carcinogen in 2009. it is still actively transmitted in endemic areas of korea, china, russia, and vietnam. currently it is estimated that more than 200 million people are at risk of infection, 15-20 million people are infected and 1.5-2 million show symptoms or complications. several molecules and genes of the fluke have been ... | 2011 | 21741496 |
| telomeric dna in chromosomes of five opisthorchid species. | the analysis of telomere repeat distribution in chromosomes of five opisthorchid species (opisthorchis felineus (rivolta, 1884), opisthorchis viverrini (poirier, 1886), metorchis xanthosomus (creplin, 1846), metorchis bilis (braun, 1890), clonorchis sinensis (cobbold, 1875)) was performed with fluorescent in situ hybridization (fish) of labeled (ttaggg)n dna-probe and pna telomere probe on mitotic and meiotic chromosomes of these species. it was shown that chromosome telomeres of all studied spe ... | 2011 | 21708281 |
| genomics and molecular genetics of clonorchis sinensis: current status and perspectives. | clonorchiasis caused by clonorchis sinensis is an important foodborne parasitosis of humans and animals, and is predominantly a hepatobiliary disease. globally, nearly 35million people were infected with c. sinensis, with approximately 15million being in china. patients would chronically present fatigue, jaundice, abdominal discomfort, along with the increased risk of developing into a form of cholangiocarcinoma that is fatal to humans. treatment of clonorchiasis by praziquantel has been very su ... | 2011 | 21704726 |
| families opisthorchiidae and heterophyidae: are they distinct? | superfamily opisthorchioidea looss, 1899 consists of three well-known families, opisthorchiidae, heterophyidae, and cryptogonimidae, with basic similarities in morphology and life-cycles. many species in the first two of these families are human pathogens, such as opisthorchis viverrini, o. felineus, clonorchis sinensis, haplorchis spp. and metagonimus spp. recently, a molecular phylogenetic study on the classification of digenea revealed a paraphyletic relationship between opisthorchiidae and h ... | 2011 | 21740979 |
| cyst formation, increased anti-inflammatory cytokines and expression of chemokines support for clonorchis sinensis infection in fvb mice. | to verify the hypothesis that different pathology of clonorchis sinensis infection by mouse strains is determined by different responses of cytokines and chemokines, we compared those responses of fvb with those of balb/c mice. all of fvb mice infected with 30 metacercariae of c. sinensis showed cystic dilatation in the liver, whereas infected balb/c mice did not. mature worms were recovered from 19 of 20 liver sections of fvb mice while only one of 20 sections of balb/c mice revealed a mature w ... | 2011 | 21820080 |
| molecular characterization of clonorchis sinensis tetraspanin 2 extracellular loop 2. | in the course of examining est of the liver fluke clonorchis sinensis, a cdna encoding the protein similar to tetraspanin 2 (tsp-2) of blood fluke schistosome was identified as cstsp2. tsps are a family of eukaryotic cell membrane-spanning proteins thought to anchor multiple proteins to one area of the cell membrane. multiple sequence alignment of cstsp2 revealed over 40% of identities with those of schistosomes. the ccc, pxsc, and cg motives characteristic to extracellular loop 2 (ec2) region o ... | 2011 | 21842393 |
| developmental transcriptomic features of the carcinogenic liver fluke, clonorchis sinensis. | clonorchis sinensis is the causative agent of the life-threatening disease endemic to china, korea, and vietnam. it is estimated that about 15 million people are infected with this fluke. c. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause cholangiocarcinoma in chronically infected individuals. accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understandin ... | 2011 | 21738807 |
| development of a taqman based real-time pcr assay for detection of clonorchis sinensis dna in human stool samples and fishes. | clonorchiasis caused by the oriental liver fluke clonorchis sinensis is a fish-borne zoonosis endemic in a number of countries. this article describes the development of a taqman based real-time pcr assay for detection of c. sinensis dna in human feces and in fishes. primers targeting the first internal transcribed spacer (its-1) sequence of the fluke were highly specific for c. sinensis, as evidenced by the negative amplification of closely related trematodes in the test with the exception of o ... | 2011 | 21729765 |
| [in vitro effect of seven anthelmintic agents against adult clonorchis sinensis]. | to observe the in vitro effect of praziquantel, tribendimidine, levamisole, artemether, artesunate, albendazole and mebendazole against adult clonorchis sinensis. | 2011 | 21823316 |
| [infection status of wild freshwater fish with metacercariae of clonorchis sinensis in west liaoning]. | a total of 1131 wild freshwater fishes were collected from nver river, gou river and liao river in the west region of liaoning province, and examined for metacercariae by direct compression and artificial digestion (pepsin-hcl) method. all the 6 species fishes were infected by clonorchis sinensis. the highest prevalence (82.8%, 216/261) were found in pseudorasbora parva and the lowest (1.5%, 2/137) in phoxinus lagowskii. the highest intensity of infection (2390 per fish) was in perccottus glehni ... | 2011 | 21826909 |
| Overview of human clonorchiasis sinensis in China. | The objective of the survey was to determine the current status, trends and transmission factors for Clonorchis sinensis infection in China and to provide updated information for development control strategies. This was part of a nationwide survey of major human parasitic diseases carried out during 2000-2002 sampled by the stratified randomized cluster sampling method. Fecal examination was conducted using the Kato-Katz thick smear method and egg count per gram of feces (EPG) was determined for ... | 2011 | 21710843 |
| identification and characterization of a novel 21.6-kda tegumental protein from clonorchis sinensis. | tegumental proteins form a membrane-bound outer surface and are thus involved in host-parasite interactions and parasite survival. a complementary dna clone encoding a novel 21.6-kda tegumental protein (cstegu21.6, accession number jf911532) was identified in a sequence library for the adult clonorchis sinensis liver fluke. the complete coding sequence was 564 bp and encoded a protein of 188 amino acids. a blastx search revealed identities from 43 to 47% with previously identified tegumental pro ... | 2011 | 22015384 |
| [evaluation of clonorchis sinensis ppmp i antigen cs2 recombinant protein for immunodiagnosis of clonorchiasis]. | to develop and preliminarily evaluate two immunodiagnostic methods for clonorchiasis using clonorchis sinensis ppmp i antigen cs2 recombinant protein (rcs2). | 2011 | 21970103 |
| molecular characterization and expression of a cysteine protease from clonorchis sinensis and its application for serodiagnosis of clonorchiasis. | cysteine proteases play essential roles in parasite physiology as well as in host-parasite interactions through their modulation of various biological and pathobiological events. in the present study, a full-length sequence encoding cysteine protease of clonorchis sinensis (cscp) was isolated from our adult cdna library. the open reading frame contains 984 bp encoding 327 amino acids. the present amino acid sequence shared 68% identity with two known cscp genes and 29-49% identity with that of o ... | 2011 | 22170263 |
| Disability Weight of Clonorchis sinensis Infection: Captured from Community Study and Model Simulation. | Clonorchiasis is among the most neglected tropical diseases. It is caused by ingesting raw or undercooked fish or shrimp containing the larval of Clonorchis sinensis and mainly endemic in Southeast Asia including China, Korea and Vietnam. The global estimations for population at risk and infected are 601 million and 35 million, respectively. However, it is still not listed among the Global Burden of Disease (GBD) and no disability weight is available for it. Disability weight reflects the averag ... | 2011 | 22180791 |
| Prevalence of Clonorchis sinensis infection in dogs and cats in subtropical southern China. | Clonorchiasis, caused by Clonorchis sinensis, is one of the major parasitic zoonoses in China, particularly in China's southern Guangdong province where the prevalence of C. sinensis infection in humans is high. However, little is known of the prevalence of C. sinensis infection in its reservoir hosts dogs and cats. Hence, the prevalence of C. sinensis infection in dogs and cats was investigated in Guangdong province, China between October 2006 and March 2008. | 2011 | 21929783 |
| a review of parasitic zoonoses in a changing southeast asia. | parasitic zoonoses are common and widely distributed in the southeast asian region. however, the interactions between parasites, hosts and vectors are influenced by environmental, socio-cultural and livestock production changes that impact on the distribution, prevalence and severity of disease. in this review we provide an update on new knowledge in the context of ongoing changes for the food-borne pig associated zoonoses taenia solium and trichinella spp., the food-borne trematodes opisthorchi ... | 2011 | 21846580 |
| [Fish and flatworms in Vietnam]. | | 2011 | 21946603 |
| rapid detection of opisthorchis viverrini copro-dna using loop-mediated isothermal amplification (lamp). | opisthorchis viverrini and other foodborne trematode infections are major health problem in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. we therefore established a rapid, sensitive and specific method for detecting o. viverrini infection from the stool samples using the loop-mediated isothermal ... | 2012 | 21871581 |
| anthelmintic effect of artesunate in experimental heterophyid infection. | artesunate is a water-soluble semi-synthetic drug derived from artemisinin which is extracted from leaves of the chinese plant artemisia annua. this successful basically-antimalarial drug has been proved to be also effective against trematodes like schistosoma japonicum, s. mansoni, fasciola hepatica and clonorchis sinensis. the present work aimed to investigate the in-vivo efficacy of artesunate against heterophyids in mice, using praziquantel as a therapeutic control. results showed that artes ... | 2011 | 21980784 |
| the draft genome of the carcinogenic human liver fluke clonorchis sinensis. | abstract: background: clonorchis sinensis is a carcinogenic human liver fluke that is widespread in asian countries. increasing infection rates of this neglected tropical disease are leading to negative economic and public health consequences in affected regions. experimental and epidemiological studies have shown a strong association between the incidence of cholangiocarcinoma and the infection rate of c. sinensis. to aid research into this organism, we have sequenced its genome. results: we c ... | 2011 | 22023798 |
| Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats. | The present study investigated the diagnostic value of an ELISA for the detection of Clonorchis sinensis antigen in the feces of experimentally infected rats. A mouse polyclonal IgG antibody against adult C. sinensis crude antigen (CsAg) was used to capture the C. sinensis coproantigen. The detection limit for pure CsAg was 20ng/ml in sample buffer and 40ng/ml in uninfected fecal extract. The test was evaluated using a follow-up of five groups of rats experimentally infected with 100, 50, 10, 5 ... | 2012 | 21864712 |
| [strengthening the research on clonorchiasis in china]. | liver flukes mainly include clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus. the international congress of liver flukes was held in khon kaen, thailand, during 7-8th march, 2011. the congress assembled a wide array of studies and reflected the current status of research, control and prevention of liver flukes in the world. this paper summarizes basic information from the meeting. meanwhile, based on the research status and needs for control and prevention, priorities of re ... | 2011 | 21970113 |
| Free radicals enzymatically triggered by Clonorchis sinensis excretory-secretory products cause NF-?B-mediated inflammation in human cholangiocarcinoma cells. | Chronic clonorchiasis, caused by direct and continuous contact with Clonorchis sinensis worms and their excretory-secretory products, is associated with hepatobiliary damage, inflammation, periductal fibrosis and even development of cholangiocarcinoma. Our previous report revealed that intracellular reactive oxygen species were generated in C. sinensis excretory-secretory product-treated human cholangiocarcinoma cells; however, their endogenous sources and pathophysiological roles in host cells ... | 2012 | 22138019 |
| correlation between discharged worms and fecal egg counts in human clonorchiasis. | stool examination by counting eggs per gram of feces (epgs) is the best method to estimate worm burden of clonorchis sinensis in infected humans. the present study investigated a correlation between epgs and worm burden in human clonorchiasis. | 2011 | 21991401 |
| [characterization and preliminary application of six monoclonal antibodies against recombinant signal protein 14-3-3 of schistosoma japonicum]. | to identify the properties of six monoclonal antibodies (mcabs) against recombinant signal protein 14-3-3 of schistosoma japonicum, and investigate their value in the diagnosis of schistosomiasis. | 2011 | 22164379 |
| Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis. | Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized their biochemical and functional properties. Multiple sequence alignment of the deduced amino acid sequences of CsLAP1 and CsLAP2 with those of other organisms revealed that typical me ... | 2011 | 22155540 |
| gene/protein expression level, immunolocalization and binding characteristics of fatty acid binding protein from clonorchis sinensis (csfabp). | clonorchis sinensis fatty acid-binding protein (csfabp) belongs to a multigene family of lipid-binding proteins and is considered to be a promising vaccine candidate for human clonorchiasis. in this study, binding characteristics of csfabp have been examined for the first time. the recombinant csfabp (rcsfabp) was found to bind 11-(dansylamino) undecanoic acid (dauda), causing a blue shift in the fluorescence emission from 543 to 531 nm with an excitation wavelength of 345 nm and a substantial i ... | 2011 | 22189506 |
| [one case with mixed infection of giardia lamblia, clonorchis sinensis, and necator americanus]. | | 2011 | 21972592 |
| Serological diagnosis of clonorchiasis: using a recombinant propeptide of cathepsin L proteinase from Clonorchis sinensis as a candidate antigen. | Clonorchiasis is a common zoonosis in southern and northeastern parts of China, especially in Guangdong, Guangxi and Jilin province. Anti-Clonorchis sinensis antibody detection by enzyme-linked immunosorbent assay (ELISA) has been used for epidemiological surveys of clonorchiasis for its convenience and celerity, but it is still a meaningful work to screen ideal diagnostic antigen or antibody subtype for improvement of diagnostic sensitivity and specificity and for judgement of curative effect. ... | 2011 | 22173451 |
| Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis. | Reversible phosphorylation of proteins is a critical mechanism involved in physiological function of organisms, including Clonorchis sinensis. In the present study, One cDNA clone encoding protein phosphatase 2A (CsPP2A) was isolated from a C. sinensis adult cDNA plasmid library. The open reading frame of the novel gene contains 924 bp and encoded a putative protein of 307 amino acids. A similarity analysis showed high homology with Schistosoma japonicum (76.3%) and Homo sapiens (84.4%), respect ... | 2011 | 22167368 |
| [Detection of Cryptospordium spp. in environmental water samples by FTA-PCR]. | To establish a FTA-polymeras chain reaction (FTA-PCR) method in detection of Cryptospordium spp. in different sources of water. | 2011 | 22164380 |
| hypersensitive reaction to praziquantel in a clonorchiasis patient. | praziquantel is the drug of choice for clonorchiasis. since clonorchiasis is endemic in most river basins, praziquantel has been widely used for 30 years in korea. a 54-year-old korean woman suffered from hypersensitive reactions, such as nausea, dyspnea, rash, and urticaria after taking the first dose of praziquantel to treat clonorchiasis. she ingested one dose again and the same symptoms appeared, and she was treated at a clinic with anti-histamines. she tried one more dose with anti-histamin ... | 2011 | 22072827 |
| genetic variation among clonorchis sinensis isolates from different geographic regions in china revealed by sequence analyses of four mitochondrial genes. | the present study examined sequence variation in four mitochondrial (mt) genes, namely cytochrome c oxidase subunits 1 (cox1) and 2 (cox2), and nadh dehydrogenase subunits 1 and 2 (nad1 and nad2) among clonorchis sinensis isolates from different endemic regions in china, and their phylogenetic relationships with other zoonotic trematodes were reconstructed. a portion of the cox1 and cox2 genes (pcox1 and pcox2), and nad1 and nad2 genes (pnad1 and pnad2) were amplified separately from individual ... | 2011 | 22152452 |
| the identification of antigenic proteins: 14-3-3 protein and propionyl-coa carboxylase in clonorchis sinensis. | clonorchis sinensis, the causative agent of clonorchiasis, is widespread in east and southeast asia, including china, vietnam and the republic of korea. we identified antigenic proteins from adult c. sinensis liver flukes using immunoproteomic analysis. in this study, we found 23 candidate antigenic proteins with a pi in the range of 5.4-6.2 in total lysates of c. sinensis. the antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glu ... | 2011 | 22119288 |
| Identification and molecular characterization of a novel signaling molecule 14-3-3 epsilon in Clonorchis sinensis excretory/secretory products. | Increasing evidence shows that 14-3-3 proteins are involved in many biology events in addition to signal transduction. Extensive investigations on structural and biochemical features of these signaling molecules have implied their importance in the biological process. In the present study, we have identified and characterized the 14-3-3 epsilon (Cs14-3-3) in Clonorchis sinensis that causes human clonorchiasis. Recombinant protein was expressed in Escherichia coli (E. coli) and identified by MALD ... | 2011 | 21922237 |
| Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata. | Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L3). However, the parasite is difficult to identify in snails. The purpose of this study was to develop a quick, simple molecular method to survey for A. cantonensis in intermediate host snails. | 2011 | 22023992 |
| Human infections of fish-borne trematodes in Vietnam: prevalence and molecular specific identification at an endemic commune in Nam Dinh province. | The prevalence of fish-borne trematodes in humans and their molecular identification was investigated in the Rang Dong commune of Nam Dinh province, Vietnam, between January 2009 and December 2010. A total of 405 people in this commune were interviewed on the habit of eating raw fish and all of their stool samples were collected using the Kato-Katz technique for examination of the presence of fish-borne trematodes. The worms (and eggs) were first morphologically examined, counted, described and ... | 2011 | 21959023 |
| Tracing of the Bile-Chemotactic Migration of Juvenile Clonorchis sinensis in Rabbits by PET-CT. | Adult Clonorchis sinensis live in the bile duct and cause clonorchiasis. It is known that the C. sinensis metacercariae excyst in the duodenum and migrate up to the bile duct through the common bile duct. However, no direct evidence is available on the in vivo migration of newly excysted C. sinensis juveniles (CsNEJs). Advanced imaging technologies now allow the in vivo migration and localization to be visualized. In the present study, we sought to determine how sensitively CsNEJs respond to bil ... | 2011 | 22180795 |
| [Purification of Clonorchis sinensis native protein and their application in immunodiagnosis of clonorchiasis]. | Lots of purified antigens have been obtained from crude antigens of Clonorchis sinensis. Some of them have applied to immunodiagnosis of clonorchiasis in laboratory. Here, we review purification methods, diagnostic efficacy and application prospect of purified antigens. | 2011 | 22164637 |
| parasitic helminth cystatin inhibits dss-induced intestinal inflammation via il-10(+)f4/80(+) macrophage recruitment. | many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). in a previous study, we isolated and characterized type i cystatin (csstefin-1) of the liver fluke, clonorchis sinensis. to investigate whether the csstefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (dss) and treated them with recombinant csstefin-1 (rcsstefin-1). the disease activity index (dai) increased in ... | 2011 | 22072824 |
| rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer pcr and melting curve analysis. | we developed a single step duplex real-time fluorescence resonance energy transfer (fret) pcr merged with melting curve analysis for the fast detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples. two species of mitochondrial nadh dehydrogenase subunit 2 (nad2) dna elements, the 165-bp nad2 product of c. sinensis and the 209-bp nad2 product of o. viverrini, were amplified by species-specific primers, and the fluorescence melting curve analyse ... | 2012 | 22246366 |
| a case of clonorchiasis with focal intrahepatic duct dilatation mimicking an intrahepatic cholangiocarcinoma. | biliary strictures can be caused by various diseases. intrahepatic duct (ihd) strictures are usually related to ihd stones and cholangitis. however, focal ihd strictures without ihd stones often create diagnostic problems. parasitic diseases such as clonorchiasis can be a rare cause of an ihd stricture. human clonorchiasis (clonorchis sinensis infection) is an endemic parasitic disease in eastern asia, including korea, and patients acquire the infestation by eating raw fish. on radiological exam ... | 2011 | 22741114 |
| [cloning, expression and evaluation on effect in serological diagnosis of cysteine protease of clonorchis sinensis]. | to clone and express the cysteine protease of clonorchis sinensis and evaluate its effect on immunodiagnosis of human clonorchiasis. | 2011 | 22379827 |
| prevalence of clonorchis sinensis metacercariae in freshwater fish from three latitudinal regions of the korean peninsula. | a large-scale survey was conducted to investigate the infection status of fresh water fishes with clonorchis sinensis metacercariae (csmc) in 3 wide regions, which were tentatively divided by latitudinal levels of the korean peninsula. a total of 4,071 freshwater fishes were collected from 3 regions, i.e., northern (gangwon-do: 1,543 fish), middle (chungcheongbuk-do and gyeongsangbuk-do: 1,167 fish), and southern areas (jeollanam-do, ulsan-si, and gyeongsangnam-do: 1,361 fish). each fish was exa ... | 2011 | 22355206 |
| [survey of clonorchiasis in fuyu county in 2009]. | a survey of clonorchis sinensis infection in residents and residents' behaviors was performed in fuyu county, 2009, the infection rate was 78.8%, and the main way of infection was eating raw fish. it is necessary to strengthen the clonorchiasis control in fuyu county. | 2011 | 22263523 |
| [effect of comprehensive control in demonstration plot of clonorchiasis in yangshan county, 2006-2009]. | to evaluate the effect of comprehensive control of clonorchiasis in demonstration plot in yangshan county so as to popularize it. | 2011 | 22263514 |
| [application and evaluation on five health education patterns of integrated control for parasitic diseases]. | to explore the strategy of health education in demonstration plots for controlling the infections of soil-transmitted helminthes (sth) and clonorchis sinensis. | 2011 | 22263497 |
| [epizootiological characteristics of clonorchiasis foci in the amur river ecosystem in the jewish autonomic region]. | malacofaunal and ichthyoparasitological studies were conducted in the floodplain-river ecosystem in the middle course of the amur river. the studies covered its channels and tributaries, such as big and small bira, bidzhan, in, and vertoprashikha rivers, in the birobidzhan, leninskoye, and smidovich districts of the jewish autonomic region in the second half of august 2011. it was established that there were biotopes of the mollusk parafossalurus manchouricus, the first intermediate host of clon ... | 2012 | 23437716 |
| the characteristics of the expression of heat shock proteins and cox-2 in the liver of hamsters infected with clonorchis sinensis, and the change of endocrine hormones and cytokines. | the liver fluke clonorchis sinensis (digenea) is a high-risk parasite that causes serious diseases such as cirrhosis, carcinogenic liver damage and clonorchiasis in east asia. this study was conducted to evaluate the relationship between stress/endocrine hormones and inflammation induced by infection as well as the expression of heat shock proteins (hsp-27, hsp-90), cox-2 and cytokines in the livers of hamsters infected with c. sinensis. the average body weight of infected hamsters decreased up ... | 2012 | 23327006 |
| production and deformation of clonorchis sinensis eggs during in vitro maintenance. | clonorchis sinensis is a carcinogenic human liver fluke. the present study monitored eggs produced by long-term maintained adult worms of c. sinensis to confirm their egg productivity in vitro. the worms from infected rabbits were incubated in vitro in 1× locke's solution and broth media (rpmi-1640, dmem and imdm). numbers of expelled eggs were counted sequentially and their morphological changes were monitored by microscopy after 1, 30, 60, and 90 days of cultivation. on the 1-3 days of cultiva ... | 2012 | 23285144 |
| the biochemical and immunological characterization of two serpins from clonorchis sinensis. | serpins (serine proteinase inhibitors) are evidenced to regulate numerous biological processes such as immunoregulation in parasitic helminths. the functions of serpins from clonorchis sinensis remain unclear to date. in this study, two serpin genes, respectively denominated as csproserpin and csserpin2, had been selected from metacercaria cdna library of c. sinensis. the biochemical activities of both recombinant proteins (rcsproserpin and rcsserpin2) were analyzed by assays of inhibition on so ... | 2012 | 23275238 |
| proteomic analysis of different period excretory secretory products from clonorchis sinensis adult worms: molecular characterization, immunolocalization, and serological reactivity of two excretory secretory antigens-methionine aminopeptidase 2 and acid phosphatase. | the excretory secretory products (esp) of clonorchis sinensis are the causative agents of clonorchiasis and biliary diseases. the parasites' esp play important roles in host-parasite interactions. the protein compositions of esp at different secretory times are different and have not been systemically investigated so far. in this study, we collected esp from six different periods (0-3 h, 3-6 h, 6-12 h, 12-24 h, 24-36 h, and 36-48 h) from c. sinensis adults. using a shotgun lc-ms/ms analysis, we ... | 2012 | 23274491 |
| prognosis of sepsis induced by cecal ligation and puncture in mice improved by anti-clonorchis sinensis cyclopholin a antibodies. | cyclophilin a (cypa), a ubiquitously distributed intracellular protein, is thought to be one of the important inflammatory factors and plays a significant role in the development process of sepsis. in the form of cytokine, cypa deteriorates sepsis by promoting intercellular communication, apoptosis of endothelial cells and chemotactic effect on inflammatory cells. in our previous study, cyclophilin a of clonorchis sinensis (cscypa), a type of excretory-secretory antigen, could induce the patient ... | 2015 | 26427806 |
| clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress. | clonorchis sinensis causes a major food-borne helminthic infection. this species locates in mammalian hepatobiliary ducts, where oxidative stressors and hydrophobic substances are profuse. to adapt to the hostile micromilieu and to ensure its long-term survival, the parasite continuously produces a diverse repertoire of antioxidant enzymes including several species of glutathione transferases (gsts). helminth gsts play pertinent roles during sequestration of harmful xenobiotics since most helmin ... | 2016 | 27296469 |
| sequence analysis and molecular characterization of clonorchis sinensis hexokinase, an unusual trimeric 50-kda glucose-6-phosphate-sensitive allosteric enzyme. | clonorchiasis, which is induced by the infection of clonorchis sinensis (c. sinensis), is highly associated with cholangiocarcinoma. because the available examination, treatment and interrupting transmission provide limited opportunities to prevent infection, it is urgent to develop integrated strategies to prevent and control clonorchiasis. glycolytic enzymes are crucial molecules for trematode survival and have been targeted for drug development. hexokinase of c. sinensis (cshk), the first key ... | 2014 | 25232723 |
| frontiers of parasitology research in the people's republic of china: infection, diagnosis, protection and surveillance. | control and eventual elimination of human parasitic diseases in the people's republic of china (p.r. china) requires novel approaches, particularly in the areas of diagnostics, mathematical modelling, monitoring, evaluation, surveillance and public health response. a comprehensive effort, involving the collaboration of 188 scientists (>85% from p.r. china) from 48 different institutions and universities (80% from p.r. china), covers this collection of 29 articles published in parasites & vectors ... | 2012 | 23036110 |
| identification and molecular characterization of parkin in clonorchis sinensis. | clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in east asian countries. parkin is a ring-between-ring protein and has e3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. a cdna clone of c. sinensis was predicted to encode a polypeptide homologous to parkin (csparkin) including 5 domains (ubl, ring0, ring1, ibr, and ring2). the cysteine and histidine residues binding to zn(2+) were all conserved and participated in ... | 2015 | 25748711 |
| serine protease inhibitors of parasitic helminths. | serine protease inhibitors (serpins) are a superfamily of structurally conserved proteins that inhibit serine proteases and play key physiological roles in numerous biological systems such as blood coagulation, complement activation and inflammation. a number of serpins have now been identified in parasitic helminths with putative involvement in immune regulation and in parasite survival through interference with the host immune response. this review describes the serpins and smapins (small seri ... | 2012 | 22310379 |
| [detection of angiostrongylus cantonensis larvae with a high resolution melt pcr assay]. | to develop a detection method of the third-stage larvae of angiostrongylus cantonensis by real-time pcr and high-resolution melt curve analysis. | 2014 | 25902677 |
| comparative evaluation of strongyloides ratti and s. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. | the use of strongyloides ratti as heterologous antigen for serodiagnosis of strongyloidiasis is preferable to strongyloides from humans due to the ease and safety of antigen preparation. in southeast asia where opisthorchis viverrini coexists with strongyloides stercoralis, there has been no report in using s. ratti for serodiagnosis of s. stercoralis. in this study, performance of an enzyme-linked immunosorbent assay (elisa) based on s. ratti was compared with that based on s. stercoralis for d ... | 2015 | 25877389 |
| [investigation on food contamination with parasites in shanghai market]. | to understand the contamination status of food with parasites in shanghai market, so as to provide the evidence for formulating the surveillance strategy in parasitic diseases and provide the technical support in the food safety. | 2012 | 23236784 |
| [analysis of larval excretory-secretory antigen and its immunodiagnosis of angiostrongyliasis cantonensis infection]. | to analyze the diagnostic value of larval excretory-secretory antigen in angiostrongylus cantonensis (lesa) infection. | 2012 | 22543125 |
| recurrent pyogenic cholangitis: a review of imaging findings and clinical management. | recurrent pyogenic cholangitis (rpc) is an infective process involving the biliary tree typified by pigmented intraductal calculi with dilatation of the intra- and extrahepatic biliary tree. previously endemic to south-east asia, rpc can now be seen in western countries with the increasing access to international travel and immigration. affected patients are often plagued by recurrent bouts of cholangitis, and commonly suffer from complications such as abscess formation and biliary strictures. i ... | 2017 | 27770158 |
| [survey of infection situation of intestinal parasites of rural residents in plain area of shandong province]. | to understand the current situation of infections of intestinal parasites of rural residents in four cities namely dongying, weifang, jining and heze of shandong province. | 2015 | 26767263 |
| prevalence and risk factors of intestinal protozoan and helminth infections among pulmonary tuberculosis patients without hiv infection in a rural county in p. r. china. | although co-infection of tuberculosis (tb) and intestinal parasites, including protozoa and helminths, in humans has been widely studied globally, very little of this phenomenon is known in china. therefore, a cross-sectional study was conducted in a rural county of china to investigate such co-infections. patients with pulmonary tb (ptb) undergoing anti-mycobacterium tuberculosis (anti-mtb) treatment were surveyed by questionnaires, and their feces and blood specimens were collected for detecti ... | 2015 | 25976412 |
| [survey of intestinal parasitic infections and related knowledge and behavior of residents in jiaodong area of shandong province]. | to understand the status of intestinal parasitic infections and the related knowledge and behavior in residents of jiaodong area of shandong province, so as to provide the evidence for making an appropriate preventive and control strategy. | 2014 | 25507731 |
| [survey of intestinal parasitic infections and related knowledge and behavior of residents in jiaodong area of shandong province]. | to understand the status of intestinal parasitic infections and the related knowledge and behavior in residents of jiaodong area of shandong province, so as to provide the evidence for making an appropriate preventive and control strategy. | 2014 | 25434133 |
| v-shaped pits in regions of ancient baekje kingdom paleoparasitologically confirmed as likely human-waste reservoirs. | in a paleo-parasitological analysis of soil samples obtained from v-shaped pits dating to the ancient baekje period in korean history, we discovered ascaris lumbricoides, trichuris trichiura, and clonorchis sinensis eggs. in light of the samples' seriously contaminated state, the v-shaped pits might have served as toilets, cesspits, or dung heaps. for a long period of time, researchers scouring archaeological sites in korea have had difficulties locating such structures. in this context then, th ... | 2014 | 25352710 |
| efficacy and safety of praziquantel, tribendimidine and mebendazole in patients with co-infection of clonorchis sinensis and other helminths. | both tribendimidine and mebendazole are broad-spectrum drugs for anti-intestinal nematodes. we aim to assess the efficacy and safety of tribendimidine and mebendazole in patients with co-infection of clonorchis sinensis and other helminths. | 2014 | 25122121 |
| intestinal parasite co-infection among pulmonary tuberculosis cases without human immunodeficiency virus infection in a rural county in china. | epidemiologic studies of co-infection with tuberculosis (tb) and intestinal parasites in humans have not been extensively investigated in china. a cross-section study was conducted in a rural county of henan province, china. pulmonary tb (ptb) case-patients receiving treatment for infection with mycobacterium tuberculosis and healthy controls matched for geographic area, age, and sex were surveyed by using questionnaires. fecal and blood specimens were collected for detection of intestinal paras ... | 2014 | 24166044 |
| the changing pattern of parasitic infection among korean populations by paleoparasitological study of joseon dynasty mummies. | in the coprolites of 4 recently discovered joseon mummies of korea, we found ascaris lumbricoides , trichuris trichiura , metagonimus yokogawai , paragonimus westermani , and clonorchis sinensis eggs. the current finding was compared with previous paleoparasitological data, and with recent national survey data from korea. for a. lumbricoides and t. trichiura , similar patterns of infection prevalence were observed between the national survey of 1961 and our current joseon data. some of the trema ... | 2014 | 24011353 |
| advances with the chinese anthelminthic drug tribendimidine in clinical trials and laboratory investigations. | the anthelminthic drug tribendimidine has been approved by chinese authorities for human use in 2004, and a first comprehensive review was published in acta tropica in 2005. here, we summarise further advances made through additional clinical trials and laboratory investigations. two phase iv trials have been conducted in the people's republic of china, the first one enrolling 1292 adolescents and adults aged 15-70 years and the second one conducted with 899 children aged 4-14 years who were inf ... | 2013 | 23352956 |