| differences in questing tick species distribution between atlantic and continental climate regions in spain. | climate and vegetation in spain vary from north to south, affecting tick distribution and consequently the presence of tick-borne diseases. the aim of this study was to investigate throughout a 2-yr study the distribution of the different exophilic questing tick species present in 18 areas: eight located in central and 10 in northern spain. the same methodology was used in both areas, sampling vegetation on a monthly basis by blanket dragging for 20- to 30-min intervals. a total of 12 species be ... | 2011 | 21337943 |
| species diversity of ixodid ticks feeding on humans in amasya, turkey: seasonal abundance and presence of crimean-congo hemorrhagic fever virus. | ticks (acari:ixodidae) are important pests transmitting tick-borne diseases such as crimean-congo hemorrhagic fever (cchf) to humans. between 2002 and 2009, numerous cchf cases were reported in turkey, including amasya province. in the current study, species diversity, seasonal abundance of ticks, and presence of cchf virus (cchfv) in ticks infesting humans in several districts of amasya province were determined. in the survey, a total of 2,528 ixodid ticks were collected from humans with tick b ... | 2011 | 21337953 |
| molecular survey of anaplasma and ehrlichia infections of feral raccoons (procyon lotor) in hokkaido, japan. | infection by anaplasma and ehrlichia in feral raccoons (procyon lotor) in hokkaido, japan, was examined by molecular methods. a polymerase chain reaction (pcr) screen for anaplasmataceae, based on 16s rrna, showed that 38 (5.4%) of 699 raccoons examined were positive. these 38 positive samples were examined for anaplasma phagocytophilum, anaplasma bovis, ehrlichia chaffeensis, and ehrlichia canis infection by species-specific nested pcr. nested pcr results indicated that 36 of the 38 samples wer ... | 2011 | 21395415 |
| surveys of rodent-borne disease in thailand with a focus on scrub typhus assessment. | the epidemiology of many rodent-borne diseases in south-east asia remains ill-defined. scrub typhus and lep-tospirosis are common and medically significant, while other zoonotic diseases, such as spotted fever group rickettsiae have been identified, but their overall medical significance is unknown. rodent surveillance was conducted from june 2002 to july 2004 in 18 provinces from thailand. traps were set up for one to three nights. blood and serum samples and animal tissue samples (liver, splee ... | 2008 | 21396076 |
| an outbreak of haemolytic anaemia associated with infection of theileria orientalis in naive cattle. | an outbreak of haemolytic anaemia occurred when 87 cattle were introduced from a presumed non-infected herd from south otago to a herd in northland (n=580 cows), new zealand, where theileriosis is endemic. | 2011 | 21409734 |
| longistatin, a plasminogen activator, is key to the availability of blood-meals for ixodid ticks. | ixodid ticks are notorious blood-sucking ectoparasites and are completely dependent on blood-meals from hosts. in addition to the direct severe effects on health and productivity, ixodid ticks transmit various deadly diseases to humans and animals. unlike rapidly feeding vessel-feeder hematophagous insects, the hard ticks feed on hosts for a long time (5-10 days or more), making a large blood pool beneath the skin. tick's salivary glands produce a vast array of bio-molecules that modulate their ... | 2011 | 21423674 |
| tick-borne bacteria in mouflons and their ectoparasites in cyprus. | the cypriot mouflon (ovis orientalis ophion), a once almost extirpated species of wild sheep, is under strict surveillance because it can be threatened by likely transmission of pathogenic bacteria, such as anaplasma spp., rickettsia spp., and coxiella burnetii, primarily from domestic ungulates. we collected 77 blood samples from cypriot mouflons and 663 of their ectoparasites (rhipicephalus turanicus, rhipicephalus sanguineus, rhipicephalus bursa, hyalomma anatolicum excavatum, hyalomma margin ... | 2011 | 21441182 |
| the hard-tick fauna of mainland portugal (acari: ixodidae): an update on geographical distribution and known associations with hosts and pathogens. | this work is an updated revision of the available information on portuguese ixodid tick species. it includes data on tick biology, ecology, taxonomy and host/pathogen-associations. the current list of portuguese ixodid ticks comprises twenty species: dermacentor marginatus (sulzer, 1776), dermacentor reticulatus (fabricius, 1794), haemaphysalis hispanica gil collado, 1938, haemaphysalis inermis birula, 1895, haemaphysalis punctata canestrini & fanzago, 1878, hyalomma lusitanicum koch, 1844, hyal ... | 2011 | 21452063 |
| [hemorrhagic fever caused by a novel tick-borne bunyavirus in huaiyangshan, china.] | background: from april to july in 2009 and 2010, unexplained severe hemorrhagic fever-like illnesses occurred in farmers from the huaiyangshan mountains range. methods: clinical specimens (blood, urine, feces, and throat swabs) from suspected patients were obtained and stored. mosquitoes and ticks in affected regions were collected. virus was isolated from 2 patients and characterized by whole genome sequencing. virus detection in additional patients and arthropods was done by virus-specific rev ... | 2011 | 21457654 |
| haemaphysalis concinna (acari: ixodida): persistent efficacy of selamectin in angora rabbits under laboratory conditions. | in the present study, the persistent efficacy of selamectin (sel) in angora rabbits infested with haemaphysalis concinna was observed. sel (6 mg/kg) was administered to rabbits with a single topical application. eighteen angora rabbits were randomly allocated to three groups of six rabbits each. at days 1, 8, 15, 22, and 29 following sel administration, rabbits were inoculated with larval, nymphal, and adult ticks and were then observed for a period of 7 days. the cumulative reduction rates at d ... | 2011 | 21472406 |
| detection of spotted fever group rickettsia in haemaphysalis longicornis in hebei province, china. | abstract dna samples from 737 ticks pools, representing 6850 haemaphysalis longicornis and 51 dermacentor nuttalli ticks collected from hebei province, china, were analyzed by pcr for the presence of spotted fever group rickettsia. there were 50 (6.9%) of 724 h. longicornis ticks pools found positive, but no positive samples found in 13 d. nuttalli. sequence analysis of the partial outer membrane protein a (ompa) genes from the ten positive samples showed 98% identity, but different from the hom ... | 2011 | 21506802 |
| arthropod symbiotes of laonastes aenigmamus (rodentia: diatomyidae). | abstract arthropod symbiotes of the laotian rock-rat, laonastes aenigmamus (rodentia: diatomyidae), from laos are examined. this host is a member of diatomyidae previously thought to have gone extinct >10 million yr ago. permanent symbiotes are represented by 2 species, a new species of sucking louse, polyplax sp., near rhizomydis (phthiraptera: polyplacidae), and a new species of fur mite, afrolistrophorus sp., near maculatus (acariformes: listrophoridae). the temporary parasites are represente ... | 2011 | 21506873 |
| biological control of engorged female haemaphysalis qinghaiensis (acari: ixodidae) ticks with different chinese isolates of beauveria bassiana. | seven isolates of the fungus beauveria bassiana (b.b) were assessed for their lethality against haemaphysalis qinghaiensis, a prevalent tick species in china. fourteen days after exposure to the isolates b.bat1, b.bat5, and b.bat7 (at 10(8) conidia ml(-1)), the mortality rate had reached 100%. the results indicated that these three b. bassiana isolates were highly virulent against the engorged female h. qinghaiensis ticks. the present study suggests that b. bassiana has potential for biocontrol ... | 2011 | 21509447 |
| detection and differentiation of borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in china. | abstract: | 2011 | 21529349 |
| cloning and characterization of the autophagy-related gene 6 from the hard tick, haemaphysalis longicornis. | autophagy is the intracellular protein degradation process which is induced by starvation. ticks have a unique tolerance for starvation, and it is possible that this tolerance is associated with their longevity. previously, we isolated the homologues of four autophagy-related (atg) genes in the hard tick, haemaphysalis longicornis, suggesting that autophagy appeared to play an important role in tolerance for starvation as well as the development of ticks. in this study, the homologue of atg6 was ... | 2011 | 21537978 |
| effect of water flooding on the oviposition capacity of engorged adult females and hatchability of eggs of dog ticks: rhipicephalus sanguineus and haemaphysalis leachi leachi. | effects of water flooding on the oviposition capacity of engorged adult females and hatchability of eggs of rhipicephalus sanguineus and haemaphysalis leachi leachi under laboratory conditions were investigated. the durations of time of water flooding were 1, 2, 4, 6, 12, 24, 48, 72, 96, and 120 hours. engorged females of r. sanguineus and h. leachi leachi did not oviposit after being flooded for more than 48 and 6 hours, respectively. the preoviposition periods of both species were longer than ... | 2011 | 21584237 |
| the importance of ixodes arboricola in transmission of rickettsia spp., anaplasma phagocytophilum, and borrelia burgdorferi sensu lato in the czech republic, central europe. | abstract wild birds are known to be a reservoir of infectious disease agents and disseminatory hosts of ticks. the purpose of this work was to obtain information about the occurrence of rickettsial, anaplasmal, and borrelial infections in some ticks that parasitize wild birds in the czech republic. a total of 549 subadult ticks of three species ixodes arboricola (75.0%), ixodes ricinus (23.1%), and haemaphysalis concinna (1.8%) were collected from 20 species of birds (passeriformes). rickettsiae ... | 2011 | 21612531 |
| rhipicephalus (boophilus) microplus embryo proteins as target for tick vaccine. | rhipicephalus (boophilus) microplus is one of the most widely distributed tick in the world. the control of the parasite is based mainly on the use of chemical acaricides, which are produced from a limited set of molecules. these drugs induce selection of acaricide-resistant ticks, and are an important source of environmental pollution. an approach based on anti-tick vaccines may circumvent these obstacles. characterization of the physiological function of tick molecules may be useful to develop ... | 2011 | 21620488 |
| identification of the heat shock protein 70 (hlhsp70) in haemaphysalis longicornis. | a haemaphysalis longicornis heat shock protein 70 (hlhsp70) was identified from a cdna library synthesized from tick eggs. the hlhsp70 cdna is 2311bp in length and encodes 661 amino acid residues with the predicted molecular weight of 72.5kda and an isoelectronic point (pi) of 5.2. it also contains the highly conserved functional motifs of the hsp70 family and a specific endoplasmic reticulum (er) retention signal "kdel" that is common among er-localized proteins. the hlhsp70 exhibits 90% amino ... | 2011 | 21621329 |
| efficacy of plant-mediated synthesized silver nanoparticles against hematophagous parasites. | the purpose of the present study was to investigate the acaricidal and larvicidal activity against the larvae of haemaphysalis bispinosa neumann (acarina: ixodidae) and larvae of hematophagous fly hippobosca maculata leach (diptera: hippoboscidae) and against the fourth-instar larvae of malaria vector, anopheles stephensi liston, japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae) of synthesized silver nanoparticles (agnps) utilizing aqueous leaf extract from musa pa ... | 2011 | 21638210 |
| [identification of haemaphysalis longicornis and rhipicephalus microplus]. | to establish a method combined morphology and molecular marker for identifying haemaphysalis longicornis and rhipicephalus microplus. | 2011 | 21781482 |
| [tick (ixodoidea) and flea (siphonaptera) species on three red foxes (vulpes vulpes) in erzurum province]. | in this study, three red foxes (vulpes vulpes) which died after traffic accidents were examined in terms of ectoparasites, in the province of erzurum in january of 2009. 13 ticks and 74 fleas were collected from the foxes and taken to the laboratory in separate glasses containing 70% alcohol. ticks were taken directly from 70% alcohol and have been identified under the stereo microscope. fleas were cleared in 10% koh solution during 4-13 days and washed in distilled water 3-4 times and were iden ... | 2011 | 21776598 |
| a survey of canine tick-borne diseases in india. | abstract: background: there are few published reports on canine babesia, ehrlichia, anaplasma, hepatozoon and haemotropic mycoplasma infections in india and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. this study investigated the occurrence and distribution of canine tick-borne disease (tbd) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in india. results: ... | 2011 | 21771313 |
| the identification and characterization of lysozyme from the hard tick haemaphysalis longicornis. | a full-length cdna-encoding lysozyme was obtained from cdna libraries of salivary glands of the hard tick haemaphysalis longicornis and designated as hllysozyme. the hllysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 121 amino acids. the calculated molecular weight of the protein is 13.7 kda, and the theoretical isoelectric point is 9.85. hllysozyme shares 41-79% amino acid sequence identity with the lysozymes of other organisms. ... | 2010 | 21771526 |
| genetic diversity of anaplasma and ehrlichia in the asian part of russia. | totally, 2590 questing adult ixodes persulcatus ticks and 1458 small mammals from ural, siberia, and the far east as well as 53 haemaphysalis concinna, 136 haem. japonica, and 43 dermacentor silvarum ticks--exclusively adults--from the far east were examined for the presence of ehrlichia and anaplasma by nested pcr based on the 16s rrna gene. both anaplasma phagocytophilum and ehrlichia muris were found in i. persulcatus and small mammals from all the studied regions. myodes spp., microtus spp., ... | 2010 | 21771512 |
| crimean-congo hemorrhagic fever virus in ticks collected from humans, livestock, and picnic sites in the hyperendemic region of turkey. | abstract during june and july 2007, about 3125 adult ticks were collected from humans, animals, and vegetation in a hyperendemic region (sivas and tokat) of turkey. a total of 2193 ticks were pooled in 225 pools and screened for the crimean congo hemorrhagic fever virus (cchfv) presence by antigen-capture enzyme-linked immunosorbent assay. infection rates were calculated as the maximum likelihood estimation with 95% confidence intervals (ci). the dominant tick species was found to be hyalomma ma ... | 2011 | 21736490 |
| spotted fever group rickettsiae in ticks in cyprus. | in two surveys conducted from march 1999 to march 2001 and from january 2004 to december 2006, a total of 3,950 ticks (belonging to ten different species) were collected from seven domestic and wild animals (goat, sheep, cattle, dog, fox, hare, and mouflon) from different localities throughout cyprus. in order to establish their infection rate with spotted fever rickettsiae (sfg), ticks were pooled and tested by polymerase chain reaction targeting glta and ompa genes, followed by sequencing anal ... | 2011 | 21833539 |
| ixodid ticks of road-killed wildlife species in southern italy: new tick-host associations and locality records. | the present study aimed to identify ticks collected from road-killed wildlife species retrieved in several localities of southern italy and to assess the presence of borrelia burgdorferi sensu lato, anaplasma phagocytophilum and rickettsia spp. dna in ticks. collections were carried out from january 2000 to december 2009 on wild animals found dead within the territories of 11 municipalities from three regions (i.e., apulia, basilicata, and calabria). in total, 189 carcasses of wild animals belon ... | 2011 | 21728058 |
| detection of borrelia burgdorferi sensu lato and anaplasma phagocytophilum in small mammals and ectoparasites in hungary. | abstract the aim of our study was to investigate the presence of borrelia burgdorferi sensu lato (s.l.) and anaplasma phagocytophilum in small mammals and ticks using polymerase chain reaction and to gain information about the prevalence and possible coexistence of these pathogens at a selected site in hungary. two hundred seventy-seven small mammals were trapped in south-eastern hungary during 2009. tissue samples and a total of 831 ectoparasites (ixodes ricinus, ixodes acuminatus, haemaphysali ... | 2011 | 21736488 |
| characterization of acid phosphatase from the tick haemaphysalis longicornis. | the full-length cdna encoding acid phosphatase (hl-3) from haemaphysalis longicornis was obtained by 5' rapid amplification of cdna ends (race). the cdna contained a 1137bp open reading frame (orf) coding for 356 amino acids with a predicted theoretical isoelectric point (pi) of 6.35 and molecular weight of 41.0kda. the recombinant protein was expressed in escherichia coli. the enzyme could hydrolyze para-nitrophenyl phosphate (pnpp) substrate at an optimum ph of 5.0. real-time rt-pcr analysis s ... | 2011 | 21715097 |
| detection of the new ehrlichia species closely related to ehrlichia ewingii from haemaphysalis longicornis in yonaguni island, okinawa, japan. | we collected a total of 206 haemaphysalis longicornis ticks by flagging in pastures in yonaguni island, okinawa, japan, in april 2008. four of the 206 tick dna samples tested were positive in a polymerase chain reaction (pcr) screening for the 16srrna gene of anaplasmataceae. partial sequences of 4 pcr products were identical to each other. longer sequences of the 16srrna gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 and 1,300 bp sequences revealed high sim ... | 2011 | 21712642 |
| coinfections of rickettsia slovaca and rickettsia helvetica with borrelia lusitaniae in ticks collected in a safari park, portugal. | borrelia and rickettsia bacteria are the most important tick-borne agents causing disease in portugal. identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. a total of 677 questing ticks including dermacentor marginatus, rhipicephalus sanguineus, ixodes ricinus, hyalomma lusitanicum, h. marginatum, and haemaphysalis punctata we ... | 2010 | 21771525 |
| Species distribution and detection of Crimean Congo Hemorrhagic Fever Virus (CCHFV) in field-collected ticks in Ankara Province, Central Anatolia, Turkey. | Ticks may act as vectors for a number of infectious diseases including Crimean Congo Hemorrhagic Fever (CCHF). The causative agent is Crimean Congo Hemorrhagic Fever Virus (CCHFV), a member of Bunyaviridae, causing extensive ecchymosis, visceral bleeding and hepatic dysfunction with a high fatality rate in the affected individuals. CCHF was initially recognized in Turkey in 2002 and the current number of reported cases exceeds 4,400. This study was conducted to confirm the presence of tick speci ... | 2012 | 21910017 |
| longistatin is an unconventional serine protease and induces protective immunity against tick infestation. | classical serine proteases use the conserved ser/his/asp catalytic triad to hydrolyze substrates. here, we show that longistatin, a salivary gland protein with two ef-hand domains from the vector tick haemaphysalis longicornis, does not have the conserved catalytic triad, but still functions as a serine protease. longistatin was synthesized in and secreted from the salivary glands of ticks, and is injected into host tissues during the acquisition of blood-meals. longistatin hydrolyzed fibrinogen ... | 2011 | 22206819 |
| Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis. | Babesiosis is one of the most important tick-borne diseases affecting livestock that can cause major economic losses worldwide particularly in the tropics. Control relies on controlling both the protozoan parasite and the tick vector. Antiprotozoal drugs are most commonly used for treatment, but problems on emergence of resistant strains and food residues are encountered. Longicin, a defensin-like peptide identified from the hard tick, Haemapysalis longicornis, as well as one of its synthetic pa ... | 2012 | 22102016 |
| Acaricidal, insecticidal, and larvicidal efficacy of fruit peel aqueous extract of Annona squamosa and its compounds against blood-feeding parasites. | Plant products may be alternative sources of parasitic control agents, since they constitute a rich source of bioactive compounds that are eco-friendly and nontoxic products. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. In the present study, fruit peel aqueous extract of Annona squamosa (Annonaceae) extracted by immersion method exhibited adulticidal activity against Haemaphysalis bispinosa (Acarina: Ixodidae) and the hematopha ... | 2011 | 22006187 |
| Seasonal abundance and activity of the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in North China. | Seasonal abundance and activity of all the three post-embryogenic stages of Haemaphysalis longicornis, both feeding and free-living phases, were evaluated over a period of 2 years, from February 2008 to January 2010, in North China. Feeding ticks were removed weekly from head and ears of domestic sheep and the attachment sites of this tick were assessed coinstantaneously; free-living ticks were collected weekly in four habitat types by flag-dragging. The results suggested that H. longicornis mai ... | 2011 | 22113778 |
| Coxiella burnetii in Western Barred Bandicoots (Perameles bougainville) from Bernier and Dorre Islands in Western Australia. | The aim of this work is to investigate the presence of Coxiella burnetii in Perameles bougainville and their ticks on two islands off Western Australia. Haemaphysalis humerosa, Haemaphysalis ratti, and Haemaphysalis lagostrophi were collected from P. bougainville on Bernier and Dorre Islands from 2005 to 2007; only Amblyomma limbatum was collected from humans over the same interval. One of 13 tick samples and 1 of 12 P. bougainville fecal samples were positive for C. burnetii DNA using quantitat ... | 2011 | 22167449 |
| Efficacy of adulticidal and larvicidal properties of botanical extracts against Haemaphysalis bispinosa, Hippobosca maculata, and Anopheles subpictus. | The aim of the present study was to investigate the adulticidal and larvicidal activity of dried leaf hexane, ethyl acetate, acetone, and methanol extracts of Nelumbo nucifera, Manilkara zapota, Ipomoea staphylina, and Acalypha indica against the adults of Haemaphysalis bispinosa (Acarina: Ixodidae), hematophagous fly Hippobosca maculata (Diptera: Hippoboscidae), and fourth instar larvae of malaria vector Anopheles subpictus (Diptera: Culicidae). Parasites were exposed to varying concentrations ... | 2011 | 21861064 |
| Multiple bites from the larvae of Haemaphysalis longicornis. | | 2011 | 22106130 |
| spotted fever group rickettsiae in ticks collected from wild animals in israel. | we report molecular evidence for the presence of spotted fever group rickettsiae (sfgr) in ticks collected from roe deer, addax, red foxes, and wild boars in israel. rickettsia aeschlimannii was detected in hyalomma marginatum and hyalomma detritum while rickettsia massiliae was present in rhipicephalus turanicus ticks. furthermore, a novel uncultured sfgr was detected in haemaphysalis adleri and haemaphysalis parva ticks from golden jackals. the pathogenicity of the novel sfgr for humans is unk ... | 2011 | 22049050 |
| cloning and characterization of a male-specific defensin-like antimicrobial peptide from the tick haemaphysalis longicornis. | a novel gene was identified from a cdna library of the male accessory glands of haemaphysalis longicornis. the full-length cdna of the gene was 349bp, encoding a 79-amino acid defensin-like protein and therefore the protein was named hlms-defensin. reverse transcriptase-pcr results suggested that this gene was expressed exclusively in male ticks. the tissue expression pattern and the mrna levels of hlms-defensin during blood feeding were determined using real-time pcr. hlms-defensin was expresse ... | 2011 | 22033149 |
| Crimean-Congo hemorrhagic fever virus in various ixodid tick species from a highly endemic area. | Ticks are major vectors of numerous diseases affecting animals and humans. Presence of various tick-borne pathogens such as Crimean-Congo hemorrhagic fever virus (CCHFV) in various tick species was documented. CCHF is a severe tick-borne illness caused by the CCHFV which is a member of the Nairovirus genus (family: Bunyaviridae). Presence of CCHFV was shown in the most prevalent ixodid tick species such as Hyalomma marginatum and Rhiphicephalus bursa in Turkey. In the present study, prevalence a ... | 2011 | 22119389 |
| ticks collected from selected mammalian hosts surveyed in the republic of korea during 2008-2009. | a tick survey was conducted to determine the relative abundance and distribution of ticks associated with selected mammals in the republic of korea (rok) during 2008-2009. a total of 918 ticks were collected from 76 mammals (6 families, 9 species) captured at 6 provinces and 3 metropolitan cities in rok. haemaphysalis longicornis (54.4%) was the most frequently collected tick, followed by haemaphysalis flava (28.5%), ixodes nipponensis (7.6%), ixodes pomerantzevi (4.8%), ixodes persulcatus (4.6% ... | 2011 | 22072840 |
| blood parasites of sheep in the netherlands. ii. babesia motasi (sporozoa, babesiidae). | summary a large babesia species occurs in sheep on the north sea islands of the netherlands. the tick haemaphysalis punctata is a vector. its pathogenicity appears to be low. it is morphologically similar to a turkish strain, considered to be b. motasi, which is also transmitted by haemaphysalis ticks. it differs from the turkish parasite serologically as well as in cross-immunity tests and in not being effective to goats. there may be a group of morphologically similar parasites with ser ... | 1980 | 22039853 |
| molecular detection of anaplasma phagocytophilum in ixodid ticks in hebei province, china. | a total of 3696 ixodid ticks, collected from hebei province, china, were examined by a nested polymerase chain reaction for the presence of anaplasma phagocytophilum. forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 haemaphysalis longicornis and four (30.8%) of 13 dermacentor nuttalli, were positive, but no significant difference was found between d. nuttalli and h. longicornis (p>0.05). sequence and phylogenetic analyses of 16s rrna gene indicated that a. phagocytophilum in ... | 2011 | 21923254 |
| scavenger receptor mediates systemic rna interference in ticks. | rna interference is an efficient method to silence gene and protein expressions. here, the class b scavenger receptor cd36 (srb) mediated the uptake of exogenous dsrnas in the induction of the rnai responses in ticks. unfed female haemaphysalis longicornis ticks were injected with a single or a combination of h. longicornis srb (hlsrb) dsrna, vitellogenin-1 (hlvg-1) dsrna, and vitellogenin receptor (hlvgr) dsrna. we found that specific and systemic silencing of the hlsrb, hlvg-1, and hlvgr genes ... | 2011 | 22145043 |
| bartonellae in animals and vectors in new caledonia. | bartonellae are gram-negative facultative intracellular alpha-proteobacteria from the family bartonellaceae. the natural history of bartonellae consists of a reservoir/host, which is a vertebrate with chronic intravascular infection with sustained bacteremia, and a vector (usually an arthropod) that transfers the bacteria from the reservoir to a susceptible yet uninfected host. in order to reveal the sources and reservoirs of bartonella infection in animals and vectors in new caledonia, we colle ... | 2011 | 22018646 |
| first molecular detection of theileria ovis in rhipicephalus sanguineus tick in iran. | to determine tick infestation of domestic ruminants and their infection to ovine theileriosis in northern iran. | 2012 | 22182639 |
| longistatin, a novel plasminogen activator from vector ticks, is resistant to plasminogen activator inhibitor-1. | thrombo-occlusive diseases are major causes of morbidity and mortality, and tissue-type plasminogen activator (t-pa) is recommended for the treatment of the maladies. however, both t-pa and u-pa are rapidly inactivated by plasminogen activator inhibitor-1 (pai-1). here, we show that longistatin, a novel plasminogen activator isolated from the ixodid tick, haemaphysalis longicornis is resistant to pai-1. longistatin was relatively less susceptible to the inhibitory effect of sds-treated platelet ... | 2011 | 21925150 |
| distribution and seasonal activity of tick species on cattle in the west aegean region of turkey. | the aim of the present study was to determine the identity, seasonal activity and distribution of tick species of cattle in the west aegean region of turkey between june 2006 and may 2008. nine villages within three provinces, viz. manisa, izmir and aydin, were included in the study and a total of 75 animal barns were visited monthly for a period of 24 months and 443 cattle were examined for the presence of ticks. it was determined that 23% of cattle were infested with ticks. a total of 19,679 a ... | 2011 | 22113777 |
| Life cycle of Haemaphysalis doenitzi (Acari: Ixodidae) under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA. | The paper investigated the life cycle of the hard tick Haemaphysalis doenitzi under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA. The results revealed that the complete life cycle of H. doenitzi requires a mean duration of 109.6 days ranging from 91 to 137 days and the average prefeeding, feeding and premoulting periods of larvae, nymphs and females and the eggs hatching period are 18.7, 26.9, 38.9, and 25.1 days, respectively. In addition, the weight of engorged femal ... | 2011 | 22113780 |
| Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii. | The Haemaphysalis longicornis longicin P4 peptide is an active part peptide produced by longicin which displays bactericidal activity against both Gram-negative and Gram-positive bacteria and other microorganisms. In the present study, the effect of the longicin P4 peptide on the infectivity of Toxoplasma gondii parasites was examined in vitro. Tachyzoites of T. gondii incubated with longicin P4 had induced aggregation and lost the trypan blue dye exclusion activity and the invasion ability into ... | 2011 | 21849158 |
| the ecology, genetic diversity and phylogeny of huaiyangshan virus in china. | surveys were carried out to better understand the tick vector ecology and genetic diversity of huaiyangshan virus (hysv) in both endemic and non-endemic regions. haemaphysalis longicornis ticks were dominant in endemic regions, while rhipicephalus microplus is more abundant in non-endemic regions. hysv rna was found in human and both tick species with more prevalence in h. longicornis and lesser in r. microplus. phylogenetic analyses indicate that hysv is a novel species of the genus phlebovirus ... | 2011 | 22190717 |
| Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting. | Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and Babesia, in which numbers of agents are highly pathogenic for cattle, sheep and goats. We developed a reverse line blot (RLB) assay for detection and differentiation of four different parasites, in which 18S ribosomal RNA gene sequence was amplified with a set of universal primers specific for all members in the genera of Theileria and Babesia; and the probes were designed on the basis of hypervariable ... | 2011 | 22143673 |
| Akt is an essential player in regulating cell/organ growth at the adult stage in the hard tick Haemaphysalis longicornis. | Ticks grow rapidly during blood feeding, and their body weight may ultimately increase 100-fold more than that before feeding. The molecular mechanisms controlling growth during blood feeding in ticks remain largely unknown. The conserved insulin/PI3K/Akt signaling pathway regulates growth and metabolism in eukaryotes. Here, we show evidence for the involvement of Akt in growth during blood feeding in the parthenogenetic strain of the hard tick Haemaphysalis longicornis. We identified a homolog ... | 2011 | 22193391 |
| blood parasites of sheep in the netherlands. i. anaplasma mesaeterum sp.n. (rickettsiales, anaplasmataceae). | summary on two occasions an anaplasm was isolated from sheep on the dutch island of ameland. the organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. treatment with oxytetracycline was effective. its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. this anaplasm differs from anaplasma ovis in that less than 30% ... | 1979 | 22040472 |
| rickettsiae in arthropods collected from the north african hedgehog (atelerix algirus) and the desert hedgehog (paraechinus aethiopicus) in algeria. | hedgehogs have become a popular pet despite their potential role in zoonotic disease transmission. we conducted an entomological study in a mountainous region of northeast algeria in which we collected 387 fleas (archeopsylla erinacei) and 342 ticks (rhipicephalus sanguineus and haemaphysalis erinacei) from paraechinus aethiopicus and atelerix algirus hedgehogs. of the hedgehogs sampled, 77.7% and 91% were infested with fleas and ticks, respectively. significantly more ticks and fleas were colle ... | 2012 | 22222114 |