| the na+/k+/2cl- cotransporter in the sea bass dicentrarchus labrax during ontogeny: involvement in osmoregulation. | this study combines a cellular and molecular analysis of the na(+)/k(+)/2cl(-) cotransporter (nkcc) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. we have characterized the complete sequence of the nkcc1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, nkcc1, and other isoforms, within the epithelium of the major osmoregulatory organs. different (absorptive and secretory) functions have been attri ... | 2006 | 17142680 |
| response of plasma and gastrointestinal melatonin, plasma cortisol and activity rhythms of european sea bass (dicentrarchus labrax) to dietary supplementation with tryptophan and melatonin. | melatonin is an effective antioxidant, immunostimulant, gonadal maturating regulator and antistress indoleamine that may be potentially useful for fish farmers. we have explored two possible ways of increasing plasma melatonin levels through the diet: direct melatonin supplementation (me diet) and supplementation with the melatonin precursor tryptophan (trp diet). to this end, a group of sea bass was fed a commercial diet (std diet) at a regular time for 16 days, after which plasma, intestine, a ... | 2007 | 17123089 |
| pituitary hormone mrna expression in european sea bass dicentrarchus labrax in seawater and following acclimation to fresh water. | the mrna expression of pituitary prolactin (prl), growth hormone (gh), somatolactin (sl), proopiomelanocortin (pomc), and gonadotropins (gthi and gthii) was quantified by real-time pcr, in sea bass, dicentrarchus labrax, adapted for 1 month to seawater (sw) or freshwater (fw). in addition, igf-i (igfi) mrna expression in liver and branchial na+/k+ -atpase activity were determined. l17 ribosomal protein (rpl17) and elongation factor 1alpha (ef1alpha) were validated as reference genes in real-time ... | 2006 | 17088417 |
| early thermal history significantly affects the seasonal hyperplastic process occurring in the myotomal white muscle of dicentrarchus labrax juveniles. | the effect of early (embryonic and larval) thermal history on subsequent (juvenile) white muscle hyperplasia was studied in a teleost fish, the european sea bass (dicentrarchus labrax l.). d. labrax, incubated and reared at constant temperatures of 13 degrees c, 15 degrees c or 20 degrees c from the embryonic stage of half epiboly up to 18-19 mm in total length, were transferred to ambient seawater temperature and reared for the subsequent 14 months on commercial feed. the somatic growth of juve ... | 2007 | 17036227 |
| effects of oleic acid on the high threshold barium current in seabass dicentrarchus labrax ventricular myocytes. | the present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (oa), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. acute application of free oa caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mv from a holding potential of -80 mv. the derived 50% inhibitory concentration (ic50) was 12.49+/-0.27 micromol l(-1). at a concen ... | 2006 | 17023597 |
| peritoneal cavity phagocytes from the teleost sea bass express a glucocorticoid receptor (cloned and sequenced) involved in genomic modulation of the in vitro chemiluminescence response to zymosan. | to gain further insight into the role of cortisol in fish innate immune responses, we cloned and sequenced a 2592bp cdna from sea bass (dicentrarchus labrax) peritoneal leukocytes (pcls) encoding a glucocorticoid receptor (dlgr1). the deduced aminoacid sequence displayed that dlgr1 belong to a multigenic family of steroid hormone receptors, and exhibited high homology (80%) to the burton's mouth breeder (haplochromis burtoni) hbgr1. the dlgr1 functional domains presented homologies with those of ... | 2007 | 16987519 |
| construction and characterization of a bac library for the european sea bass dicentrarchus labrax. | | 2006 | 16978191 |
| a cdna for european sea bass (dicentrachus labrax) 11beta-hydroxylase: gene expression during the thermosensitive period and gonadogenesis. | steroid p450 11beta-hydroxylase, encoded by the cyp11b gene, is a key mitochondrial enzyme for the production of 11-oxygenated androgens, which have been shown to be potent masculinising steroids in several fish species. in this study we have isolated a cyp11b cdna of 1903 base pairs from the testis of european sea bass (dicentrarchus labrax) encoding a predicted protein of 552 amino acids. the amino acid identities to other vertebrate 11beta-hydroxylase proteins ranged from 66% to 72% to other ... | 2007 | 16962121 |
| effect of photoperiod on the fish innate immune system: a link between fish pineal gland and the immune system. | the pineal gland via its secretory product, melatonin, influences the light-dark rhythm in most vertebrates including fish. apart from the information concerning this circadian rhythm, the interrelation of the melatonin with other physiological processes has not been considered in fish. thus, we evaluated the changes in the humoral innate immune system of seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) specimens exposed to a constant light-dark photoperiod (12 hr l:12 hr d). s ... | 2006 | 16948787 |
| associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (dicentrarchus labrax). | seabass were fed for 4 months with diets where the lipid was provided as either canola oil (co), palm oil (po) or fish oil (fo), to generate diversity in their tissue fatty acid (fa) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. in particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (oa, 18:1n-9 and la, 18:2n-6, respectivel ... | 2006 | 16916978 |
| effect of temperature on maximum swimming speed and cost of transport in juvenile european sea bass (dicentrarchus labrax). | this study is an attempt to gain an integrated understanding of the interactions between temperature, locomotion activity and metabolism in the european sea bass (dicentrarchus labrax). to our knowledge this study is among the few that have investigated the influence of the seasonal changes in water temperature on swimming performance in fish. using a brett-type swim-tunnel respirometer the relationship between oxygen consumption and swimming speed was determined in fish acclimatised to 7, 11, 1 ... | 2006 | 16916977 |
| effects of using slurry ice during transportation on the microbiological, chemical, and sensory assessments of aquacultured sea bass (dicentrarchus labrax) stored at 4 degrees c. | slurry ice, a biphasic system consisting of small spherical ice crystals surrounded by seawater at subzero temperature, was evaluated as a new chilled storage method for whole sea bass (dicentrarchus labrax) a sparidae fish species of remarkable commercial interests. in this study two different group of chilling methods were used during transportation; in slurry ice packaged (group a), and flake ice packaged (group b). the effect of this advanced system during transportation on quality losses an ... | 2006 | 16864138 |
| rapid metabolic adaptation in european sea bass (dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress. | a study was conducted to evaluate the effect of two dietary carbohydrate sources (waxy maize starch and glucose) on the metabolic adaptation of sea bass juveniles (initial weight: 24 g) to a heat shock treatment (temperature rise from 18 degrees c to 25 degrees c within 24 h). two isonitrogenous and isolipidic diets were formulated to contain 20% waxy maize starch (ws diet) or 20% glucose (glu diet). triplicate groups of fish were fed to near satiation for 4 weeks at both temperatures (18 degree ... | 2006 | 16807027 |
| influence of the scge protocol on the amount of basal dna damage detected in the mediterranean mussel, mytilus galloprovincialis. | genotoxicity studies using the single cell gel electrophoresis (scge) assay indicate that basal levels of dna strand breaks (sbs) in marine invertebrates are higher and more variable than those in marine vertebrates. this elevated level of dna damage was attributed to a large number of alkali-labile sites, which are characteristic of the tightly-packaged dna in invertebrate cells. to investigate if altering the scge protocol can artificially modulate high levels of sbs, scge experiments were per ... | 2006 | 16795087 |
| cloning of the promoter from the gonadal aromatase gene of the european sea bass and identification of single nucleotide polymorphisms. | aromatase contributes to sex differentiation by catalysing the conversion of androgens into estrogens. we have cloned the promoter of the gonadal aromatase gene from the sea bass, dicentrarchus labrax, a species whose farming is complicated by male-skewed sex ratios. the promoter shows a conserved binding site for sf-1 as well as two camp response elements (cres) and putative binding sites for transcription factors belonging to the sox and forkhead families. analysis of promoter sequences from i ... | 2006 | 16782377 |
| growth dynamics of white muscle fibres in relation to somatic growth of larvae of european sea bass (dicentrarchus labrax, l.). | the body length and diameter of muscle fibres from the right hypaxial tail myomeres were measured in 100 sea bass larvae (dicentrarchus labrax, l.) at ages of 40 to 90 days, from a fish farm in croatia. the larvae were kept at a temperature of 19-20 degrees c and fed with standard feed for their respective ages. body lengths of larvae 40-90 days old varied from 8 to 42 mm, increasing significantly in 10-day intervals (p < 0.001), except between days 50 and 60 when there was no increase in the bo ... | 2006 | 16755364 |
| a transcriptomic approach of salinity response in the euryhaline teleost, dicentrarchus labrax. | euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). this physiological capacity is generally monitored using enzyme activity assays (na+/k+ -atpase...), hormones quantification (prolactine, growth hormone) or their mrnas expression. to date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. in the sea bass dicentrarchus labrax, genetic differentiation was ... | 2006 | 16737785 |
| est-based identification of genes expressed in the liver of adult seabass (dicentrarchus labrax, l.). | the scarcity of the genomic resources for some fish species, in spite of their commercial interest, could retard the positive effects that modern biotechnology can offer to aquaculture industry. then an effort should be made to reduce, as far as it concerns genomic resources, the gap that separates farming species from "model organisms". in this paper, we present an est project in which we performed single pass sequencing on 1229 randomly selected clones from a sea bass cdna library. the sequenc ... | 2006 | 16616996 |
| digestive tract ontogeny of dicentrarchus labrax: implication in osmoregulation. | the ontogeny of the digestive tract (dt) and of na(+)/k(+)-atpase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost dicentrarchus labrax reared at two salinities: seawater and diluted seawater. histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. at hatching, the dt is an undifferentiated straight tube over the yolk sac. at the mo ... | 2006 | 16573732 |
| intake of high levels of vitamin a and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in european sea bass (dicentrarchus labrax). | the effect of the feeding period on larval development was investigated in european sea bass larvae by considering the expression level of some genes involved in morphogenesis. larvae were fed a control diet except during three different periods (period a: from 8 to 13 d post-hatching (dph); period b: from 13 to 18 dph; period c: from 18 to 23 dph) with two compound diets containing high levels of vitamin a or pufa. european sea bass morphogenesis was affected by these two dietary nutrients duri ... | 2006 | 16571146 |
| the effects of biological fouling control at coastal and estuarine power stations. | the use of low levels of oxidants as antifouling agents in coastal and estuarine power station cooling water circuits can give rise to concerns over the likely production of chlorination byproducts (cbps) including organohalogens, of largely cryptic identity, loading and impact. in order to review the current understanding of the degree of impact involved this paper describes a recent collaborative programme of work on cbps within the wider context of a number of allied studies on power station ... | 2006 | 16497340 |
| effects of experimental terbuthylazine exposure on the cells of dicentrarchus labrax (l.). | the effects of acute exposure to the herbicide terbuthylazine (3.55, 5.01 and 7.08 mg l(-1)) on the cells of farmed european sea bass, dicentrarchus labrax l., were investigated by means of light and electron microscopy. in gills of treated fish, the number of chloride cells (ccs) and rodlet cells (rcs) increased significantly within 24 h and 48 h, respectively; the intestine showed the largest increase in rcs linked to treatment and exposure time. in kidney, 24 h exposure induced a significant ... | 2006 | 16487568 |
| biotransformation, stress and genotoxic effects of 17beta-estradiol in juvenile sea bass (dicentrarchus labrax l.). | the effects of 17beta-estradiol (e(2)) on fish became a matter of concern, since significant levels of this hormone were detected in the aquatic environment released mainly by domestic sewage treatment plants. in this perspective, the current study was focused on e(2) effects upon biotransformation, stress and genotoxic responses of juvenile dicentrarchus labrax l. (sea bass). fish were exposed to e(2) during 10 days in two different ways: water diluted (200 ng/l or 2,000 ng/l) and i.p. injected ... | 2006 | 16483653 |
| effects of chemical lipid extraction and arithmetic lipid correction on stable isotope ratios of fish tissues. | for accurate interpretation of fish trophodynamics from carbon stable isotope data it is necessary to extract tissue lipids. this is because lipid content varies within and among tissues in both space and time, and because lipids are 13c-depleted relative to proteins. however, lipid extraction may affect delta15n, thus requiring costly and time-consuming separation of delta13c and delta15n analyses. these problems have prompted the development of arithmetic correction techniques for delta13c, bu ... | 2006 | 16429479 |
| blood chemistry and histological properties of wild and cultured sea bass (dicentrarchus labrax) in the north adriatic sea. | histological and biochemical procedures were employed to study liver histomorphology and plasma levels of aspartate aminotransferases (ast), alanine aminotransferase (alt), triglyceride (trig), cholesterol (chol), glucose (glu) and total protein (tp) in cultured (csb) and wild sea bass (wsb) (dicentrarchus labrax) from the northern part of the adriatic sea. histopathological changes in liver included varying degrees of infiltration and lipid degeneration of hepatocytes in examined cultured fish. ... | 2005 | 16369882 |
| effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in european sea bass (dicentrarchus labrax) juveniles. | we determined the effect of dietary starch on growth performance and feed utilization in european sea bass juveniles. data on the dietary regulation of key hepatic enzymes of the glycolytic, gluconeogenic, lipogenic and amino acid metabolic pathways (hexokinase, hk; glucokinase, gk; pyruvate kinase, pk; fructose-1,6-bisphosphatase, fbpase; glucose-6-phosphatase, g6pase; glucose-6-phosphate dehydrogenase, g6pd; alanine aminotransferase, alat; aspartate aminotransferase, asat and glutamate dehydro ... | 2006 | 16343962 |
| impact of acute cadmium exposure on the trunk lateral line neuromasts and consequences on the "c-start" response behaviour of the sea bass (dicentrarchus labrax l.; teleostei, moronidae). | behavioural responses of sea bass dicentrarchus labrax were investigated after exposure to cadmium ions in laboratory-controlled conditions. the aim of this study was to discover whether environmental exposure to cadmium ions inactivates fish lateral line system neuromasts, and to determine the behavioural consequences of such a sensory blockage. for this, fish escape behaviour in response to an artificial water jet was recorded using a 25-frames s(-1) analog video camera before and after cadmiu ... | 2006 | 16324755 |
| cellular activities during a mixed leucocyte reaction in the teleost sea bass dicentrarchus labrax. | in this investigation a number of "in vitro" activities of sea bass peripheral blood leucocytes (pbl) against allogeneic pbl inactivated by irradiation were studied. stimulator pbl were cultured with inactivated allogeneic pbl, and direct counting of lymphocytes was done after 2 weeks by immunofluorescence and flow cytometry using mabs dlt15 and dlig3 specific for t-cells and b-cells, respectively. in a one-way mixed leucocyte reaction (mlr), results showed an increase of t lymphocytes, whereas ... | 2006 | 16293420 |
| oral serotonin administration affects the quantity and the quality of macronutrients selection in european sea bass dicentrarchus labrax l. | teleost fish are able to regulate their energy intake selecting from pure macronutrients sources, but the regulatory mechanisms involved in macronutrients selection remain unknown. serotonin (5-ht) reduces food intake in mammals and fish and modifies the macronutrients selection pattern in mammals; however, no information is available about its role on macronutrients selection in fish. the aim was to determine the effect of orally administered 5-ht (0.1, 0.5 and 2.5 mg kg bw(-)(1)) into gelatine ... | 2006 | 16257018 |
| production and characterization of a continuous embryonic cell line from sea bass (dicentrarchus labrax l.). | continuous cell lines represent an important tool both for biological studies and for their applications in marine biotechnology. in this article we describe the production and characterization of a continuous adherent cell line, named dlec, derived from early embryos of the european sea bass dicentrarchus labrax l. (actinopterygii, moronidae). cells were obtained by disrupting 2- to 12-hour-old embryos and culturing resulting cells at 18 degrees c in rpmi medium containing 5% fetal calf serum ( ... | 2013 | 16249966 |
| differential freshwater adaptation in juvenile sea-bass dicentrarchus labrax: involvement of gills and urinary system. | the effects of long-term freshwater acclimatization were investigated in juvenile sea-bass dicentrarchus labrax to determine whether all sea-bass juveniles are able to live in freshwater and to investigate the physiological basis of a successful adaptation to freshwater. this study particularly focused on the ability of sea-bass to maintain their hydromineral balance in freshwater and on their ion (re)absorbing abilities through the gills and kidneys. two different responses were recorded after ... | 2005 | 16215214 |
| cdna encoding sequences for myostatin and fgf6 in sea bass (dicentrarchus labrax, l.) and the effect of fasting and refeeding on their abundance levels. | fish have the ability to compensate for set-backs in growth as a result of fasting. when food levels are restored, growth in these fish can increase over and above normal rates. this phenomenon, known as "compensatory growth", has been studied with respect to enhancing food conversion efficiency. however, the mechanisms by which food intake activates an increase in somatic growth, and especially in muscle growth, are not well understood. in this study, we report first on the isolation of two com ... | 2006 | 16183242 |
| muscle cellularity at cranial and caudal levels of the trunk musculature of commercial size sea bass, dicentrarchus labrax (linnaeus, 1758). | in eight specimens of atlantic sea bass of commercial size (congruent with 350 g) muscle cellularity was studied at two selected sampling levels of the trunk axial musculature: caudal (anal opening) and cranial (fourth radius of the dorsal fin). the following parameters were quantified at both sampling levels: white muscle cross-sectional area, white muscle fibre diameter (900-1200 fibres), muscle fibre number and muscle fibre density. results showed a higher total cross-sectional area at crania ... | 2005 | 16159368 |
| adaptive bone formation in acellular vertebrae of sea bass (dicentrarchus labrax l.). | mammalian bone is an active tissue in which osteoblasts and osteoclasts balance bone mass. this process of adaptive modelling and remodelling is probably regulated by strain-sensing osteocytes. bone of advanced teleosts is acellular yet, despite the lack of osteocytes, it is capable of an adaptive response to physical stimuli. strenuous exercise is known to induce lordosis. lordosis is a ventrad curvature of the vertebral column, and the affected vertebrae show an increase in bone formation. the ... | 2005 | 16155222 |
| dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in european sea bass dicentrarchus labrax larval development. | we evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain pufa (lc-pufa) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in european sea bass larvae. fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of epa and dha. diet names indicated the percentage of epa and dha contained in the phospholipids ... | 2005 | 16149740 |
| quantification of a glucocorticoid receptor in sea bass (dicentrarchus labrax, l.) reared at high stocking density. | to assess the effects of environmental stress conditions on fish, the examination of a suite of biomarkers, including endocrine parameters, has been suggested. in teleosts, glucocorticoids, including cortisol and corticosterone, are known to mediate stress response. inside the cell, they bind to a high-affinity cytosolic glucocorticoid receptor (gr), which acts as ligand-dependent transcription factor to control and regulate gene expression. receptor number or affinity may directly influence the ... | 2005 | 16129569 |
| dietary protein source affects lipid metabolism in the european seabass (dicentrarchus labrax). | the study was undertaken to evaluate the effects of dietary protein sources on lipogenesis and fat deposition in a marine teleost, the european seabass (dicentrarchus labrax). four isonitrogenous (crude protein (cp, nx6.25), 44% dm) and isoenergetic (22-23 kj/g dm) diets were formulated to contain one of the following as the major protein source: fish meal (fm), one of two soy protein concentrates (spc) and corn gluten meal (cgm). apparent digestibility coefficients of the diets and raw ingredie ... | 2005 | 16087375 |
| in vivo allograft rejection in a bony fish dicentrarchus labrax (l.): characterisation of effector lymphocytes. | lymphoid cell subpopulations involved in allograft rejection in the teleost dicentrarchus labrax were characterised at the ultrastructural level and quantified by using monoclonal antibodies against t- and b-lymphocytes. t-cells positive for t-cell receptor beta-chain (tcr beta) were detected by reverse transcription/polymerase chain reaction (rt-pcr) and in situ hybridisation by using rna probes for tcr beta. flow cytometry detected a similar percentage of t- and b-lymphocytes (around 17%) in t ... | 2005 | 16047156 |
| effect of periodical water current on the phasing of demand feeding rhythms in sea bass (dicentrarchus labrax l.). | the demand feeding rhythms of sea bass (dicentrarchus labrax l.) maintained at rest or subjected to exercise (increase in swimming activity) as a result of an induced water current are compared. both sea bass kept individually and in group (4 and 8 individuals) shifted their food demands towards the exercise period or to just after the same (the latter especially in individually kept fish). the origin of such a response is discussed in view of innate behaviour, the energy cost of locomotor activ ... | 2005 | 15985274 |
| husbandry stress during early life stages affects the stress response and health status of juvenile sea bass, dicentrarchus labrax. | in aquaculture management it is important to establish objective criteria to assess health and welfare of the fish. here we show that european sea bass (dicentrarchus labrax) confronted with husbandry-associated stress (tank cleaning, i.e. scrubbing, and water temperature variation) during early life stages show poorer survival and disease resistance as juveniles. we evaluated several parameters for stress (plasma cortisol, glucose and lactate, hydromineral status), growth performance, the immun ... | 2006 | 15961320 |
| a microsatellite linkage map of the european sea bass dicentrarchus labrax l. | a genetic linkage map of the european sea bass (dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. the mapping panel was derived from farmed sea bass from the north adriatic sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). a total of 162 microsatellites were mapped in 25 linkage groups. eleven loci represent type i (coding) markers; 2 loci are located within the peptide ... | 2005 | 15937133 |
| the polarisation of hair cells from the ear of the european bass (dicentrarchus labrax). | the polarisation of ciliary bundles on the macula of the saccule in the european bass (dicentrarchus labrax l) has been studied using a scanning electron microscope (sem). these data show that d. labrax possesses ciliary bundles arranged in four dichotomous quadrants with a standard orientation, comparable to hearing generalists from the order perciformes. the spacing between ciliary bundles was investigated in three size classes of fish, with the results indicating that the addition of receptor ... | 2005 | 15922641 |
| high-pressure effects on the proteolytic enzymes of sea bass (dicentrarchus labrax l.) fillets. | high-pressure processing is a nonthermal technique ensuring food product safety and enabling a longer shelf life. the purpose of this experiment was to evaluate the effect of high pressure on the main proteolytic enzymes involved in fish muscle degradation during storage. enzymes were extracted with sarcoplasmic proteins from dicentrarchus labrax sea bass white muscle. activity of cathepsins b, d, h, and l was quantified in protein extract, whereas calpain activity was evaluated after isolation ... | 2005 | 15884825 |
| cardiac preload and venous return in swimming sea bass (dicentrarchus labrax l.). | cardiac preload (central venous pressure, p(cv), mean circulatory filling pressure (mcfp), dorsal aortic blood pressure (p(da)) and relative cardiac output (q) were measured in sea bass (dicentrarchus labrax) at rest and while swimming at 1 and 2 bl s(-1). mcfp, an index of venous capacitance and the upstream venous pressure driving the return of venous blood to the heart, was measured as the plateau in pcv during ventral aortic occlusion. compared with resting values, swimming at 1 and 2 bl s(- ... | 2005 | 15879073 |
| sea bass (dicentrarchus labrax) androgen receptor: cdna cloning, tissue-specific expression, and mrna levels during early development and sex differentiation. | androgens play key roles in vertebrate sex differentiation, gonadal maturation and reproductive behaviour and their actions are generally mediated through specific nuclear receptors. the present study describes the isolation, sequencing and characterization of the cdna encoding the androgen receptor (ar) in the european sea bass. ar was cloned from a sea bass testis cdna library and encoded a predicted protein of 767 residues, with a calculated molecular weight of 86.4 kda and a theoretical pi o ... | 2005 | 15878229 |
| lordotic vertebrae in sea bass (dicentrarchus labrax l.) are adapted to increased loads. | lordosis in fish is an abnormal ventral curvature of the vertebral column, accompanied by abnormal calcification of the afflicted vertebrae. incidences of lordosis are a major problem in aquaculture and often correlate with increased swimming activity. to understand the biomechanical causes and consequences of lordosis, we mapped the morphological changes that occur in the vertebrae of european sea bass during their development from larva to juvenile. our micro-ct analysis of lordotic and non-lo ... | 2005 | 15863108 |
| genetic, endocrine, and environmental components of sex determination and differentiation in the european sea bass (dicentrarchus labrax l.). | the european sea bass (dicentrarchus labrax l.) is a differentiated gonochoristic marine teleost of the family moronidae (closely related to the hermaphrodites of the family serranidae), where many juvenile males exhibit intratesticular oocytes, suggesting a certain sexual lability. like most fish, the sea bass does not have recognizable heterochromosomes or sex-linked markers but there are clear parental effects on the sex ratios. the data available so far indicate that the proportion of female ... | 2005 | 15862554 |
| fish macronutrient selection through post-ingestive signals: effect of selective macronutrient deprivation. | recent reports describe teleosts as being able to regulate energy intake by selecting from pure macronutrient sources, although the regulatory mechanisms involved in this selection remain unknown. the aim of the present work was to determine the effect of selective macronutrient deprivation on energy regulation and macronutrient selection, using for this purpose carbohydrate (ch), protein (p), and fat (f) packaged separately into gelatin capsules, a method that prevents the diet chemosensory pro ... | 2005 | 15811401 |
| quantitative aspects of the spatial distribution and morphological characteristics of the sea bass (dicentrarchus labrax l.; teleostei, serranidae) trunk lateral line neuromasts. | the results presented herein report quantitative data relative to the distribution and morphological characteristics of both types of neuromasts encountered on the trunk lateral line of the sea bass (dicentrarchus labrax, l.). these data were obtained from scanning electron micrographs. they indicate that, as expected, each modified scale of the sea bass possessed a single canal neuromast with long axis oriented parallel to the fish's long axis. in contrast to several fish species, two thirds of ... | 2005 | 15761216 |
| molecular characterization of three peroxisome proliferator-activated receptors from the sea bass (dicentrarchus labrax). | peroxisome proliferator-activated receptors (ppar) are nuclear hormone receptors that control the expression of genes involved in lipid homeostasis in mammals. we searched for ppar in sea bass, a marine fish of particular interest to aquaculture, after hypothesizing that the physiological and molecular processes that regulate lipid metabolism in fish are similar to those in mammals. here, we report the identification of complementary dna and corresponding genomic sequences that encode three dist ... | 2004 | 15726823 |
| maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers. | to investigate the origin and maintenance of the genetic discontinuity between atlantic and mediterranean populations of the common sea bass (dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. the result were also compared with new or previously published microsatellite data. seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between atlantic and mediterranean populations that matches a ... | 2005 | 15669962 |
| molecular markers for animal biotechnology: sea bass (dicentrarchus labrax, l.) hmg-coa reductase mrna. | modern technologies may improve fish production and quality and, at the same time, reduce environmental impact with benefits on the public perception of the industry. to be economically profitable, these modern technologies request an increase of rearing density that, however, could affect fish welfare. with the aim to search for molecular biomarkers to describe fish welfare, we have recently compared gene expression of sea bass farmed at different population densities by differential display ob ... | 2005 | 15656995 |
| morphofunctional ontogeny of the urinary system of the european sea bass dicentrarchus labrax. | european sea bass (dicentrarchus labrax) are euryhaline fish that tolerate wide salinity fluctuations owing to several morphofunctional adaptations. among the osmoregulatory sites (tegument, branchial chambers, digestive tract, urinary system), little is known about the kidney and the urinary bladder. the present study describes the ontogeny of the urinary system (kidney and urinary bladder) and focuses on the progressive expression of the na(+)/k(+)-atpase in the cells of these ion-transporting ... | 2005 | 15616825 |
| binding characteristics and daily rhythms of melatonin receptors are distinct in the retina and the brain areas of the european sea bass retina (dicentrarchus labrax). | melatonin is synthesized, with a circadian rhythm, in the pineal organ of vertebrates, high levels being produced during the scotophase and low levels during the photophase. the retina also produces melatonin, although in the case of the european sea bass, its secretion pattern appears to be inverted. in the study described here, radioreceptor assay techniques were used to characterize the melatonin binding sites, their regional distribution and their daily variations. brain and retina membrane ... | 2004 | 15542079 |
| dietary tag source and level affect performance and lipase expression in larval sea bass (dicentrarchus labrax). | the influence of dietary tag source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mrna level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain tag. growth was not ... | 2004 | 15506240 |
| a model for acute iron overload in sea bass (dicentrarchus labrax l.). | evaluation of several parameters involved in iron metabolism was carried out after intraperitoneal (i.p.) injection with iron dextran (idx) in sea bass (dicentrarchus labrax l.). after treatment, a rapid mobilization of idx from the peritoneal cavity to other organs was observed. this was followed by a modification of normal peripheral blood iron parameters. total iron (ti) and transferrin saturation (ts) rose rapidly, to 4.14 microg/ml and 83.7%, respectively, on day 3. in contrast, unsaturated ... | 2004 | 15479557 |
| rearing density influences the expression of stress-related genes in sea bass (dicentrarchus labrax, l.). | the worldwide decline of ocean fisheries stocks has provided a rapid growth in fish farming and the problems connected to animal welfare in aquaculture gained importance. in this context, we have looked for molecular markers among those genes whose expression could reasonably result modified by the different farming conditions. with this purpose, we have evaluated, in liver and brain of sea basses, grown for 3 months at different biomass density (<10, 80 and 100 kg/m3), the expression of those g ... | 2004 | 15474294 |
| sensory evaluation of sea bass (dicentrarchus labrax l.) fed two diets differing in fat content. | | 2004 | 15372963 |
| vitamin e in early stages of sea bass (dicentrarchus labrax) development. | this study reports titration of vitamin e levels in the sea bass (dicentrarchus labrax) using high-pressure liquid chromatography. the first part of the work is devoted to vitamin e detection in: (1) plasma of maturing females and males characterized by different body sizes; (2) seminal fluid and eggs; and (3) developing embryos of sea bass fed with vitamin e. in the second part of the study, variations of vitamin e levels during larval development are analyzed. the results show a direct correla ... | 2004 | 15369832 |
| juvenile sea bass biotransformation, genotoxic and endocrine responses to beta-naphthoflavone, 4-nonylphenol and 17 beta-estradiol individual and combined exposures. | juvenile sea bass, dicentrarchus labrax l., were exposed during 2, 4, 8, and 24 h to 0.9 microm beta-naphthoflavone (bnf), 131 nm 17 beta-estradiol (e(2)), 4.05 microm 4-nonylphenol (np), as well as to bnf combined either to e(2) or np (maintaining the previous concentrations). liver cytochrome p450 content (p450), ethoxyresorufin-o-deethylase (erod), and glutathione s-transferase (gst) activities were measured in order to evaluate biotransformation responses. genotoxicity was assessed as erythr ... | 2004 | 15294438 |
| cloning, characterisation, and expression of three oestrogen receptors (eralpha, erbeta1 and erbeta2) in the european sea bass, dicentrarchus labrax. | three oestrogen receptor [er] subtypes have been described in teleost fish, namely eralpha, and two erbeta subtypes, called erbeta1 and erbeta2 (or erbeta and ergamma in atlantic croaker). their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. we cloned three oestrogen receptors from adult liver (sb-eralpha cdna) and ovary (partial sb-erbeta1 and sb-erbeta2 cdnas) of the european sea ... | 2004 | 15279912 |
| oral administration of melatonin reduces food intake and modifies macronutrient selection in european sea bass (dicentrarchus labrax, l.). | seasonal and circadian changes have been observed in dietary selection and feeding behavior of fish. it is known that the light-dark cycle is the principal mediator of the production of pineal-derived melatonin in fish, and also that the digestive tract synthesizes and secretes melatonin and indeed is the principal extrapineal source of this secretor product, suggesting that melatonin could be involved in the processes of feeding and/or digestion. fish are capable of regulating their energy inta ... | 2004 | 15230867 |
| immunolocalisation of vasoactive intestinal peptide and substance p in the developing gut of dicentrarchus labrax (l.). | this study was carried out on the sea bass (dicentrarchus labrax) to follow, during development, the appearance and distribution of substance p (sp) and vasoactive intestinal peptide (vip), which act on gut motility. the results suggest that sp and vip play an important role as neuromodulators, influencing the motility of the digestive tract starting from the early stages of gut development, even prior to exotrophic feeding. in the peptidergic nervous system, the appearance of immunoreactivity t ... | 2008 | 15208087 |
| cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of p450 aromatase in juvenile sea bass (dicentrarchus labrax). | the teleost brain is characterized by exceptionally high levels of aromatase, the enzyme that converts androgens into estrogens, and by its continuous growth throughout life. gonadal estrogens have been implicated in sex differentiation and the control of reproduction in adult fish, but the role of neural estrogens during early development is far from clear. the present study describes the isolation and characterization of the cdna sequence from brain aromatase (p450aromb) in the european sea ba ... | 2004 | 15149730 |
| modulation of the activity of sea bass (dicentrarchus labrax) head-kidney macrophages by macrophage activating factor(s) and lipopolysaccharide. | the aim of this study was to establish the requirements for macrophage activating factor (maf) production by sea bass head-kidney leucocytes and the kinetics of macrophage activation when exposed to maf-containing supernatants and/or lipopolysaccharide (lps), a known macrophage stimulant. maf activity was found in culture supernatants of total head-kidney leucocytes pulsed with 5 microg ml(-1)con a, 5 or 10 ng ml(-1)pma and 100 ng ml(-1)calcium ionophore, or 10 microg ml(-1)con a alone, as asses ... | 2004 | 15123313 |
| melatonin binding sites in the brain of european sea bass (dicentrarchus labrax). | characteristics, day-night changes, guanosine 5'-o-(3-thiotriphosphate) (gtpgammas) modulation, and localization of melatonin binding sites in the brain of a marine teleost, european sea bass dicentrarchus labrax, were studied by radioreceptor assay using 2-[(125)i]iodomelatonin as a radioligand. the specific binding to the sea bass brain membranes was rapid, stable, saturable and reversible. the radioligand binds to a single class of receptor site with the affinity (kd) of 9.3 +/-0.6 pm and tot ... | 2004 | 15118230 |
| fatty acid pattern, oxidation product development, and antioxidant loss in muscle tissue of rainbow trout and dicentrarchus labrax during growth. | the levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (oncorhynchus mykiss) and sea basses (dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. the present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in viv ... | 2004 | 15113162 |
| evaluation of biomarkers in caged fishes and mussels to assess the quality of waters in a bay of the nw mediterranean sea. | specimens of sea bass (dicentrarchus labrax) were placed in cages for 1 month in spring and autumn at different locations in the bay of cannes (nw mediterranean). biochemical markers evaluated were: ethoxyresorufin o-deethylase (erod) and glutathione s-transferase (gst) activities in fish livers and acetylcholinesterase (ache) activity in fish muscle. erod and gst activities were higher in front of the outlet for the wastewater plant of cannes and in the harbour than outside the marina. high ero ... | 1998 | 15093298 |
| beta-naphthoflavone alters normal plasma levels of vitellogenin, 17 beta-estradiol and luteinizing hormone in sea bass broodstock. | a variety of environmental pollutants exhibit antiestrogenic properties, i.e. these compounds antagonize estrogen-dependent processes in their target tissues. this is the case with beta-naphthoflavone (beta nf), a compound that is known for its ability to bind to the arylhydrocarbon receptor (ahr) and thereby to induce cytochrome p4501a (cyp1a) expression. in previous studies, we have shown that beta nf antagonizes the 17 beta-estradiol (e2)-mediated production of vitellogenin (vtg) in cultured ... | 2004 | 15084410 |
| bioavailability of oxytetracycline in sea bass, dicentrarchus labrax (l.). | | 2004 | 15009254 |
| comment on "biomagnification study on organochlorine compounds in marine aquaculture: the sea bass (dicentrarchus labrax) as a model". | | 2004 | 14998046 |
| formation of the egg envelope of a teleost, dicentrarchus labrax (l.): immunochemical and cytochemical detection of multiple components. | the formation of the egg envelope in a teleost, dicentrarchus labrax (l.), was analysed at histological and ultrastructural level. the sequential deposition of three main layers (z1, z2 and z3) constitutes the extracellular matrix throughout oocyte development. various findings indicate that these subunits are biochemically distinct: (1) periodate- and phosphotungstic acid-reactive carbohydrates are obviously detected only in the z1, that constitutes the initial deposit of the egg envelope in ea ... | 2004 | 14991400 |
| analysis of the sox gene family in the european sea bass (dicentrarchus labrax). | sox (sry-related genes containing a hmg box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. twenty sox genes are present in the genomes of humans and mice, but far less is known about the sox gene family in other vertebrate types. we have obtained clones representing the hmg boxes of twelve sox genes from european sea bass (dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed ... | 2004 | 14990224 |
| effect of photoperiod manipulation on the daily rhythms of melatonin and reproductive hormones in caged european sea bass (dicentrarchus labrax). | reproduction in fish is cyclical and timed to guarantee the survival of the offspring. seasonal variations in reproductive hormones of fish have been deeply investigated in fish over the last years. however, there are few studies regarding the daily changes in reproductive hormone profiles in teleosts. the aim of the present research was to investigate the effects of photoperiod manipulation on melatonin and reproductive hormones (pituitary sbgnrh, pituitary lh and plasma lh, testosterone [t], a ... | 2004 | 14980798 |
| occurrence of acth- and enkephalin-like peptides in the developing gut of dicentrarchus labrax l. | we carried out immunohistochemical tests in the developing gut of the sea bass dicentrarchus labrax to follow the appearance and distribution of the immunoreactivity (ir) to antibodies against pomc-derived, adrenocorticotropic hormone (acth), alpha-melanocyte stimulating hormone (alpha-msh) and beta-endorphin (beta-end), and against two enkephalins, with the aim to study a possible involvement of these molecules in the early neuro-immune-endocrine integration. our data show that ir to antibodies ... | 2004 | 14980793 |
| postmortem degradation of white fish skeletal muscle (sea bass, dicentrarchus labrax): fat diet effects on in situ dystrophin proteolysis during the prerigor stage. | all during fish postmortem evolution, structural muscle proteins are targets for various proteases. during the prerigor period (24 hours at 4 degrees c for sea bass), cytoskeletal proteins are affected by the first proteolytic events. these cleavages disrupt connections between myofibrils and the extracellular matrix, induce segmentation of myofibril cores, and modify the rheological properties of tissue. dystrophin, a cytoskeletal actin-binding protein, is a relevant in situ marker for muscular ... | 2001 | 14961380 |
| milli-calpain from sea bass (dicentrarchus labrax) white muscle: purification, characterization of its activity and activation in vitro. | a calcium-activated neutral cysteine protease was purified to homogeneity from dicentrarchus labrax white muscle using three steps: hydrophobic interaction, anion exchange, and gel filtration chromatographies. the purified enzyme showed a native molecular weight of 124 kda with an oligomeric structure (large subunit of 80 kda and small subunit of 24 kda). it has been classified as a milli-calpain from its calcium sensitivity. activity was maximal at ph 7.0, 24 degrees c in tris buffer without na ... | 2002 | 14961288 |
| adenosine triphosphate concentration and beta-d-glucuronidase activity as indicators of sea bass semen quality. | the most common parameters used to evaluate sperm quality are motility rate and duration and fertilization ability. in this study, chemical and biochemical parameters of sea bass (dicentrarchus labrax) sperm were investigated to find an alternative method for evaluating sperm fertilization ability before and after cryopreservation. the biochemical and chemical analyses were performed with fresh and frozen-thawed sperm and seminal plasma. to cryopreserve sperm, 250-microl straws were used. fertil ... | 2004 | 14960478 |
| lernanthropus kroyeri infections in farmed sea bass dicentrarchus labrax: pathological features. | twenty sea bass dicentrarchus labrax l. from a fish farm (floating cage) in greece were examined for the presence of parasites. the gills of 7 (35%) fish were infected with adult female specimens of the parasitic copepod lernanthropus kroyeri van beneden, 1851, and the intensity of infection ranged from 1 to 24 parasites per host. the most infected portion of the gills appeared to be the primary lamellae. erosion, desquamation and necrosis of the secondary lamellae were noticed near the site of ... | 2003 | 14735939 |
| development and linkage relationships for new microsatellite markers of the sea bass (dicentrarchus labrax l.). | twenty-eight polymorphic microsatellites were isolated from the sea bass, dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. analysis for these markers and 11 recently described microsatellites of d. labrax found linkage between 26 loci and revealed eight linkage groups. | 2004 | 14731231 |
| cloning and expression of gonadotropin-releasing hormone receptor in the brain and pituitary of the european sea bass: an in situ hybridization study. | a full-length cdna encoding a gnrh receptor (gnrh-r) has been obtained from the pituitary of the european sea bass, dicentrarchus labrax. the complete cdna is 1814 base pairs (bp) in length and encodes a protein of 416 amino acids. the 5' utr and 3' utr are 239 bp and 324 bp in size, respectively. the expression sites of this gnrh-r were studied in the brain and pituitary of sea bass by means of in situ hybridization. a quantitative analysis of the expression of the gnrh-r gene along the reprodu ... | 2004 | 14724132 |
| evaluation of dna damage in dicentrarchus labrax sperm following cryopreservation. | in this paper, dna laddering analysis and single-cell gel electrophoresis (scge) or comet assay, were used to detect dna damage in response to a cryopreservation process in sea bass spermatozoa. the results obtained demonstrate that the cryopreservation protocol used to cryopreserve the sea bass sperm cause significantly damage at dna level. in fact, the degree of dna damage in frozen-thawed sperm (%dnat=38.2+/-11.2, mt=498.9+/-166.4, n=3) was different (p<0.01) from that measured in fresh sperm ... | 2003 | 14697734 |
| immunoglobulin protein and gene transcripts in ovarian follicles throughout oogenesis in the teleost dicentrarchus labrax. | transfer of immunoglobulins (igm-like) from the female to the teleost embryo has been demonstrated but mechanisms of uptake into and storage within the eggs remain to be clarified. the monoclonal antibody dlig3 against dicentrarchus labrax ig light chain revealed an active role of both follicle cells and oocytes in the ig uptake. the primordial follicular cells showed dlig3 immunoreactivity even at a pre-vitellogenetic stage. early vitellogenetic oocytes (lipid vesicle stages) had dlig3 staining ... | 2004 | 14658064 |
| induction of tetraploid gynogenesis in the european sea bass (dicentrarchus labrax l.). | a preliminary study on tetraploid gynogenetic induction in the european sea bass was performed by pressure-blocking the second polar body release and the first cleavage in eggs fertilized with ultraviolet-irradiated sperm. fertilization of eggs with genetically inactivated sperm produced only haploid development that terminated around hatching. pressure treatments (8.500 psi for 2 min) applied at 6 and 65 min after fertilization (a.f.) produced variable levels (7-95%) of tetraploid larvae at hat ... | 2003 | 14620962 |
| response to confinement in sea bass (dicentrarchus labrax) is characterised by an increased biosynthetic capacity of interrenal tissue with no effect on acth sensitivity. | two experiments were carried out to investigate the influence of confinement stress on plasma cortisol levels and on the sensitivity of the interrenal cells to adrenocorticotropic hormone (acth) stimulation in sea bass dicentrarchus labrax. confining sea bass at 70 kg m(-3) for 24 h resulted in elevated plasma cortisol levels at all times (0.1, 1, 4 and 24 h) and corresponded to a reduced cortisol content in head-kidney homogenates after 0.1 and 1 h of confinement. an increased activity of the i ... | 2003 | 14613789 |
| spatial distribution and morphological characteristics of the trunk lateral line neuromasts of the sea bass (dicentrarchus labrax, l.; teleostei, serranidae). | the morphology and spatial distribution of the different types of neuromasts encountered on the trunk lateral line of the sea bass (dicentrarchus labrax) were examined using scanning electron microscopy. the sea bass trunk lateral line exhibits a complete straight pattern. in their basic features, the two types of neuromasts present, canal and superficial, resemble what has been described in other fishes. they are similar in their general cellular organization but differ in sizes, and shapes, as ... | 2003 | 14573996 |
| oxidative defenses in the sea bass, dicentrarchus labrax. | a study on the oxidative defenses during larval growth and under stress conditions was carried out in the bred sea bass, dicentrarchus labrax. a high-pressure liquid chromatography (hplc) method was used for the quantitative determination of vitamins c and e; glutathione peroxidase activity was measured by an enzymatic assay. vitamin e was measured in the seminal fluid, eggs, embryos and larvae. vitamins c and e, and glutathione peroxidase activity were measured in adults of dicentrarchus labrax ... | 2003 | 14562766 |
| immunopurification of b lymphocytes from sea bass dicentrarchus labrax (l.). | the monoclonal antibody dlig3, specific for immunoglobulins and b cells of the teleost fish dicentrarchus labrax (mediterranean sea bass), was used to enrich immunoreactive cells from peripheral blood, spleen, and head kidney leukocytes. the purification was performed by immunomagnetic sorting of leukocyte fractions enriched by percoll density gradient centrifugation, and the purity of the isolated cells was estimated by immunofluorescence and cytofluorimetric analysis. following a single immuno ... | 2014 | 14509263 |
| effect of two thermal regimes on the muscle growth dynamics of sea bass larvae, dicentrarchus labrax l. | muscle growth was studied in larvae of sea bass, dicentrarchus labrax l., reared at two temperatures: real ambient temperature ( congruent with 15 degrees c during vitelline phase and increased gradually) and 19 degrees c from fertilization until the end of larval development. muscle cellularity, body length and body weight were measured. early temperature influenced larval development and so, pre-larval phase finished earlier at 19 degrees c than at ambient temperature (4 and 6 days, respective ... | 2003 | 12969026 |
| molecular characterization of sea bass gonadotropin subunits (alpha, fshbeta, and lhbeta) and their expression during the reproductive cycle. | reproduction is controlled by two pituitary gonadotropin hormones, follicle-stimulating hormone (fsh) and luteinizing hormone (lh). this study reports the cloning, sequence analysis, and gene expression of gonadotropin (gth) subunits from the european sea bass (dicentrarchus labrax). the gth subunits were cloned from a sea bass brain-pituitary cdna library. the nucleotide sequences of the common alpha, the fshbeta, and the lhbeta subunit cdnas were 625, 521, and 591 base pair (bp) long, respecti ... | 2003 | 12928011 |
| effect of dietary phospholipid level and phospholipid:neutral lipid value on the development of sea bass (dicentrarchus labrax) larvae fed a compound diet. | the aim of the study was to determine the influence of dietary phospholipid concentration on survival and development in sea bass (dicentrarchus labrax) larvae. larvae were fed from day 9 to day 40 post-hatch with an isoproteic and isolipidic formulated diet with graded phospholipid levels from 27 to 116 g/kg dm and different phospholipid:neutral lipid values. the best growth (32 mg at the end of the experiment) survival (73 %) and larval quality (only 2 % of malformed larvae) were obtained in t ... | 2003 | 12844371 |
| aromatase activity in the european sea bass (dicentrarchus labrax l.) brain. distribution and changes in relation to age, sex, and the annual reproductive cycle. | cytochrome p450 aromatase activity (aa) was measured in different tissues of the sea bass (dicentrarchus labrax l.) using a tritiated water release assay that was previously optimized and validated for this species. in adult fish entering the reproductive season, aa was highest on a per mg protein basis, in the brain (2.04+/-0.4 pmol/mg prot/h; mean+/-sem), followed by the ovary (0.59+/-0.1) and was detectable in visceral fat (0.21+/-0.05), liver (0.08+/-0.009), and head kidney (0.03+/-0.004). h ... | 2003 | 12812769 |
| genotoxicity biomarkers' association with b(a)p biotransformation in dicentrarchus labrax l. | dicentrarchus labrax (sea bass) were exposed during 0, 4, 8, 16, 24, 48, and 96 h to 0 and 0.1 microm benzo(a)pyrene (b(a)p), an environmental pollutant, and the following biomarkers were measured: (1) liver cytochrome p450 (p450) content and ethoxyresorufin-o-deethylase (erod) activity as phase i biotransformation parameters, (2) liver gluthathione-s-transferase (gst) activity as a phase ii biotransformation conjugation enzyme, (3) biliary and liver cytosolic b(a)p-type metabolites by fixed wav ... | 2003 | 12798770 |
| dicentrarchus labrax biotransformation and genotoxicity responses after exposure to a secondary treated industrial/urban effluent. | the present research work was designed to study dicentrarchus labrax (sea bass) biotransformation and genotoxicity responses to the soluble fraction of a secondary treated industrial/urban effluent (sf-stiue) discharged through a submarine pipe outlet into the aveiro coastal area. sea bass was exposed for 4, 8, 16, 24, 48, and 96 h to 0%, 0.1%, and 1% sf-stiue and the following biological responses were measured: (1) liver cytochrome p450 (p450) content and ethoxyresorufin-o-deethylase (erod) ac ... | 2003 | 12798764 |
| growth and hepatic acetyl coenzyme-a carboxylase activity are affected by dietary protein level in european seabass (dicentrarchus labrax). | two trials were undertaken with european seabass (dicentrarchus labrax) to estimate the protein requirements for maintenance and growth as well as the effect of dietary protein level on the activity of hepatic acetyl coenzyme-a carboxylase (acoac). six diets were formulated to contain graded levels of protein (from 5 to 55% crude protein (cp)) at a constant (12%) lipid level. three other diets were also formulated to contain 35, 45 and 55% cp, but with a higher lipid level (19%). groups of 10 in ... | 2003 | 12781985 |
| cloning of a proopiomelanocortin cdna from the pituitary gland of the sea bass (dicentrarchus labrax) and assessment of mrna expression in different tissues by means of real-time pcr. | proopiomelanocortin (pomc) cdna was cloned from sea bass (dicentrarchus labrax) pituitary gland. a 743 nucleotide sequence was obtained coding for the following sequences flanked by sets of proteolytic cleavage sites: acth (ser(88)-met(127)), alpha-msh (ser(88)-gly(102)), clip (pro(106)-met(127)), beta-lph (glu(131)-gln(208)), gamma-lph (glu(131)-ser(175)), beta-msh (asp(159)-ser(175)), and beta-endorphin (tyr(178)-gln(208)). no region homologous to gamma-msh/joining peptide (a tetrapod pomc fea ... | 2003 | 12630925 |
| neurohypophysial hormone regulation of cl- secretion: physiological evidence for v1-type receptors in sea bass gill respiratory cells in culture. | neurohypophysial hormone receptors were studied in primary cultures of sea bass (dicentrarchus labrax) gill respiratory-like cells grown on permeable supports. this preparation was previously shown to provide a functional model for investigating the hormonal regulation of cl- secretion. under control conditions, the cultured monolayered epithelium had a short-circuit current (isc) of 3.5+/-1.1 micro a x cm(-2). this current had previously been identified as an active cl- secretion. the addition ... | 2003 | 12525255 |
| toxicity and pathological effects of orally and intraperitoneally administered ivermectin on sea bass dicentrarchus labrax. | the toxicity and histopathology of ivermectin was studied in 3 and 35 g sea bass dicentrarchus labrax l. following in-feed, oral intubation and injection administration at dose rates ranging from 0.5 to 3.5 mg kg(-1). estimated ld50 values for 3 g fish were 0.335 and 0.106 mg kg(-1) following oral intubation and injection administration respectively, for fish reared at 11 degrees c; and 0.839 and 1.023 mg kg(-1) following oral intubation and injection administration, respectively for fish reared ... | 2002 | 12517007 |
| juvenile sea bass liver biotransformation and erythrocytic genotoxic responses to pulp mill contaminants. | this research represents the first study concerning liver phase i biotransformation induction, measured as cytochrome p-450 (p450) and ethoxyresorufin-o-deethylase (erod), and genotoxic responses, measured as erythrocytic micronuclei (emn) and nuclear abnormalities (ena), in dicentrarchus labrax l. (sea bass) exposed to pulp and paper mill contaminants. juvenile sea bass were exposed for 6 h to 0 (control) and 0.0125 microm concentrations of the resin acids (ras) abietic acid (aa) and dehydroabi ... | 2002 | 12481865 |
| protection of peroxide-treated fish erythrocytes by coelenterazine and coelenteramine. | european seabass (dicentrarchus labrax) erythrocytes treated with tert-butyl hydroperoxide (t-bhp) showed decreasing levels of reduced glutathione, increased lipid peroxidation and dna damage, and ultimately underwent haemolysis. the addition of the marine luciferin coelenterazine (clzn) markedly delayed the onset of the haemolytic process induced by t-bhp as well as lipid peroxidation and glutathione oxidation. clzn also protected the red blood cells' dna against t-bhp-triggered damage. clzn's ... | 2002 | 12448822 |