| [changes of the expression of histocompatibility (hla) antigens in human fibroblasts transformed by the avian leukosis-sarcoma virus]. | monoclonal monomorphic antibodies anti-hla class i and ii antigens, were used to evaluate the expression of these molecules on normal and rsv-transformed human fibroblasts. the results indicate that the human diploid fibroblasts transformed in vitro by rsv present a reduced hla-class i antigens expression as compared to the uninfected fibroblasts of the same donor. in parallel, it is demonstrated that the class ii molecules absent on normal cells are expressed after transformation. | 1985 | 3000539 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| characterization of a growth-inhibiting protein present in rat serum that exerts a differential effect on in vitro growth of nonmalignant rat liver cells when compared with rous sarcoma virus-transformed rat liver cells. | we have previously reported the transformation by rous sarcoma virus of a cloned epithelial cell line (brl) established from buffalo rat liver by h. coon. the nontransformed (brl) and transformed (rsv-brl) cells grew at comparable rates in culture, whereas only the transformed cells were tumorigenic in vivo. we report here on the existence in rat and mouse sera of a growth inhibitor for the nontransformed brl cells. the transformed brl cells (rsv-brl) were insensitive to this inhibitor. the inhi ... | 1985 | 3003021 |
| tissue-specific expression in transgenic mice of a fused gene containing rsv terminal sequences. | transgenic mice were generated with prsv-cat, a chimeric gene construct containing the long terminal repeat of rous sarcoma virus (rsv) linked to the bacterial gene encoding chloramphenicol acetyltransferase (cat). cat expression, detected in adult animals of five independent strains, was preferentially directed to organs rich in tendon, bone, and muscle. this pattern reflects the disease specificity of the intact virus and suggests that the tissue tropism of rsv is determined at least in part b ... | 1986 | 3006249 |
| rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation. | mutagenesis of glycine 2 of p60src, the transforming protein of rous sarcoma virus (rsv), yields a protein that is neither myristylated nor bound to cellular membranes. although these mutant viruses retain full tyrosine protein kinase activity, they are transformation-defective. we examined in detail tyrosine phosphorylation of cellular polypeptides and the phenotype induced by infection with two such viruses. infection failed to cause growth in agar, cytoskeletal reorganization, or changes in f ... | 1986 | 3006923 |
| intracellular k+ and the expression of transformation parameters by chick cells transformed with the bryan strain of rous sarcoma virus. | as normal chick embryo (ce) cells entered quiescence the intracellular concentrations of both na+ and k+ declined. comparable decreases in intracellular concentrations of na+ and k+ were not observed in ce cells transformed by either the schmidt-ruppin (sr) or the bryan (b) strain of rous sarcoma virus (rsv). intracellular concentrations of na+ were higher in sr-rsv-transformed ce cells than in b-rsv-transformed cells and uninfected ce cells at all times after plating. in contrast, intracellular ... | 1986 | 3008417 |
| ecto-protein kinase activities in normal and transformed cells. | the incubation of intact uninfected and rous sarcoma virus (rsv)-transformed chicken cells (sr-rsv-a) with micromolar amounts of [gamma-32p]atp under physiological conditions resulted in the radioactive phosphorylation of a variety of proteins. according to the experimental protocol the detectable phosphorylation was restricted to atp utilization at the cell surface and was catalyzed by surface located protein kinase (pk). serine- and to a lesser extent, threonine residues were phosphorylated. w ... | 1986 | 3009192 |
| genetic structure of the endogenous proviruses and expression of the gag gene in brown leghorn chickens. | seven loci of endogenous proviruses were detected in the genome of brown leghorn chickens. sets of endogenous proviruses in dna of the chicken embryos examined were identified by blot hybridization with 32p-labelled dna of rsv and ecori restriction endonuclease digestion. comparison of the results showed that only one locus (a) of endogeneous provirus was associated with a gs+ phenotype as determined by the immunoperoxidase reaction and antibodies against gag gene products of rsv. restriction en ... | 1986 | 3009239 |
| tests of association of immunoglobulin allotype genes and viral oncogenesis in chickens. | chickens from regional poultry research laboratory (rprl) inbred line 6(3) are resistant to virally-induced marek's disease (md) and lymphoid leukosis (ll) and are relatively strong regressors of virally-induced rous sarcomas. in contrast, rprl line 100 chickens are highly susceptible to md and ll and are weaker regressors of rous sarcomas than line 6(3). rprl lines 100 and 6(3) differ for alleles at the igg-1 (g-1) allotype locus, but have identical igm-1 (m-1) allotype alleles. to test the pos ... | 1986 | 3009316 |
| transcriptional activation encoded by the v-fos gene. | we present evidence that the fos oncogene encodes a transcriptional trans-activation function. trans-activation was assayed by cotransfection into nih 3t3 mouse fibroblasts of v-fos dna containing plasmids together with a plasmid containing a test promoter. three v-fos dnas were used: (i) pfbr-1, a plasmid containing the fbr proviral sequences; (ii) pfbj-2, a plasmid harboring the fbj proviral sequences; (iii) pmf-j, a plasmid containing the fbj fos sequences linked to a mouse metallothionein pr ... | 1986 | 3010284 |
| an anticatalytic monoclonal antibody to avian plasminogen activator: its effect on behavior of rsv-transformed chick fibroblasts. | a monoclonal antibody has been raised against the serine protease, plasminogen activator (pa) produced by rous sarcoma virus-transformed chick embryo fibroblasts (rsvcef), and selected for its ability to inhibit the catalytic activity of pa. the high specificity and anticatalytic nature of the antibody has allowed probing of the direct role of pa in cellular behavior. microgram quantities of monoclonal igg inhibit the overgrowth and the morphological changes associated with rsvcef transformation ... | 1986 | 3011282 |
| activation of the pp60c-src kinase during differentiation of monomyelocytic cells in vitro. | the proto-oncogene c-src, the cellular homolog of the rous sarcoma virus (rsv) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. to obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human hl-60 leukaemia cell line as a mo ... | 1986 | 3011421 |
| expression of integrated rous sarcoma viruses: dna rearrangements 5' to the provirus are common in transformed rat cells but not seen in infected but untransformed cells. | the study of rous sarcoma virus (rsv)-infected rat cell clones offers a novel approach to unravelling the mechanisms controlling eukaryotic gene expression. rsv-transformed rat cell clones frequently contain duplicated proviral sequences immediately upstream of an intact provirus. this category of proviral rearrangement is not seen in cells that remain untransformed after rsv infection nor in subsequently segregating transformants. these results suggest that such rearrangements occur during or s ... | 1986 | 3011422 |
| the use of rous sarcoma virus transformation mutants with differing tyrosine kinase activities to study the relationships between vinculin phosphorylation, pp60v-src location and adhesion plaque integrity. | tyrosine-specific phosphorylation of cellular proteins has been implicated in the neoplastic transformation of cells by rous sarcoma virus (rsv). one of the putative substrates for the src gene product (pp60v-src) of rsv is the cytoskeletal protein vinculin, giving rise to the hypothesis that tyrosine-specific phosphorylation of vinculin disrupts adhesion plaque integrity, leading to the characteristic rounded morphology of rsv-transformed cells. we have investigated this hypothesis by analysing ... | 1986 | 3011478 |
| [viral respiratory infections in children: new diagnostic methods for early detection. initial results of a pilot project in switzerland]. | the new methods of rapid viral diagnosis make it possible to specify a number of the most prevalent agents of respiratory tract infections within 24 hours. the techniques are based on the immunological detection of antigens of respiratory syncytial (rsv), adeno, parainfluenza type 1, 2 and 3, as well as of influenza a and b viruses in nasopharyngeal secretions. during a one-year period we have used these methods to evaluate diagnostically 1541 outpatients presenting with upper and lower respirat ... | 1986 | 3012772 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| [virus-specific proteins in cells transformed by the chicken sarcoma virus d6]. | the structural proteins and the oncogene product of the avian sarcoma virus (asv) d6 were analyzed by the radioimmunoprecipitation. adv d6 was obtained from the chemically induced tumour. asv d6 contains all the structural proteins found in rsv, but some of them (p19, gp 85) differ from those of rsv. the product of asv d6 oncogene is pp65src. the protein kinase activity of this src protein is identical to that of wt pp60src. the nature of some other proteins, which can be phosphorylated in this ... | 1986 | 3013582 |
| virus-specific nucleotide sequences in duck cells transformed by chicken and duck-adapted rous sarcoma virus. | adaptation of pr-rsv-c on duck cells results in successful and efficient replication of the adapted virus in duck cells. the adapted variant, dapr-rsv-c, was compared with the parental chicken-cell derived pr-rsv-c. no differences in the efficiency of integration and in the number of integrated proviral copies in duck cells were found. however, the structure of proviral dna of the adapted virus was different. whereas ecori and hindiii digestion showed no differences between chicken-cell derived ... | 1986 | 3013702 |
| characterization of monoclonal antibodies to avian leukosis viruses. | hybridoma cell lines secreting monoclonal antibody (mca) to avian leukosis virus (alv) structural proteins p27 and p19 have been established. in an indirect enzyme-linked immunosorbent assay (elisa), mca 6al20 (igg1 isotype) reacted with rpl-40 (alv subgroup a), avian myeloblastosis virus (amv) (a mixture of subgroups a and b), rous-associated virus (rav)-2 (subgroup b), and carr-zilber strain of rous sarcoma virus (cz-rsv) (subgroup d) but not with prague strain of rsv (prc-rsv) (subgroup c) or ... | 1986 | 3015099 |
| d-galactosyltransferase and its endogenous substrates in chick embryo fibroblasts transformed by rous sarcoma virus. | udp-d-galactose: 2-acetamido-2-deoxy-beta-d-glucopyranosyl 4-beta-d-galactosyltransferase (galtase) activity was purified, from primary chick embryo fibroblast (cef) transformed by a temperature-sensitive, rous sarcoma virus mutant (cef-rsv), by chromatography on an affinity resin prepared with monoclonal antibodies to galtase. cellular glycopeptides from cef, as well as cef-rsv, maintained at permissive (37 degrees) [cef-rsf (37 degrees)] and nonpermissive temperatures (41 degrees) [cef-rsv (41 ... | 1986 | 3015407 |
| transplantable primate tumors induced by rous sarcoma virus. i. induction of tumors transplantable into young marmosets. | the tumors induced in white-lipped marmosets (saguinus fuscicollis, s. nigricollis) by rous sarcoma virus (rsv) of chicken origin (rsv-sr) were not transplantable to allogeneic hosts. in contrast, rsv rescued from these tumors (rsv-m) induced sarcomas that were transplantable to young but not to adult marmosets. the tumors induced by rsv-m and the transplants rapidly enlarged, metastasized to various organs, and killed the recipients 29-59 days post inoculation. cell lines were readily establish ... | 1986 | 3016397 |
| detection of transcription and translation in situ with biotinylated molecular probes in cells transfected with recombinant dna plasmids. | the efficiency of transfection and subsequent expression of recombinant dna plasmids in monolayers of cv-1 monkey kidney cells was analyzed by immunoperoxidase and in situ hybridization with biotin-nucleotide-labeled dna molecular probes. two recombinant plasmids were used for transfection. both contained the 3' long terminal repeat (ltr) of rous sarcoma virus (rsv) as the transcriptional promoter, but two different coding sequences were employed [bacterial chloramphenicol acetyltransferase (prs ... | 1986 | 3017147 |
| 31p nmr spectra of rodent and avian fibroblasts transformed by rous or kirsten sarcoma viruses. | 31p nmr spectra of normal rodent and avian fibroblasts were compared to those of the same cells transformed either by the rous sarcoma virus (rsv) or by the kirsten sarcoma virus (ki-msv). under physiological conditions, the spectra of living or perchloric acid extracted chicken embryo fibroblasts, rat cell line fr3t3 and mouse cell line c127 did not differ from those of their counterparts transformed by rsv or ki-msv. however, in the case of fr3t3 cells, on shifting from 37 degrees c to 20 degr ... | 1986 | 3017336 |
| cleavage of vimentin in dense cell cultures. inhibition upon transformation by type 5 adenovirus. | the analysis on two-dimensional isoelectric focusing and sds polyacrylamide gels (2d gels) of the triton x-100 and high salt-insoluble fraction of fibroblast cell lines, certain epithelial cell lines and granulosa cells revealed various amounts of a vimetin cleavage product, with a more basic pi and with a mw (1,500-2,000) lower than that of intact vimentin. this cleavage product of vimentin which constituted as much as 30% of the total vimentin in an established rat embryo fibroblast cell line ... | 1986 | 3017738 |
| regulation of cellular morphology by the rous sarcoma virus src gene: analysis of fusiform mutants. | we have been interested in how rous sarcoma virus (rsv) influences transformed cell morphology and compared the molecular properties of chicken embryo cells (cec) infected with mutants of rsv that induce the fusiform transformed cell morphology with those of cec infected by wild-type rsv, which induces the more normal round transformed cell morphology. we looked for properties shared by all fusiform mutant-infected cells, because these may be responsible for maintaining the fusiform morphology. ... | 1985 | 3018500 |
| expression strategies of the yeast retrotransposon ty: a short sequence directs ribosomal frameshifting. | the ty element of yeast is a member of a class of eukaryotic transposons which bear a striking resemblance to retroviral proviruses in their structure and expression strategies (1,2). a direct comparison can be drawn between the production of a fusion protein encoded by ty, resulting from a frameshift event which fuses two out-of-phase open reading frames tya and tyb, and the production of pr180gag-pol in a retrovirus such as rous sarcoma virus (rsv) (3,4). we present data which shows, definitiv ... | 1986 | 3020502 |
| [structural polymorphism of the provirus integrated in the genome of mammalian cells transformed by rous sarcoma virus]. | the structural organization of integrated rous sarcoma virus (rsv) genome in 8 different mammalian tumour cell lines has been studied. the different types of provirus rearrangements were found, e.g.: breakpoint mutations, deletions of rsv structural genes, elimination of the entire viral genome. the structural changes of proviruses appeared during long-term cultivation of tumour cell lines in vitro. the ability of transformed cells to produce complete infectious rsv correlate with the structural ... | 1986 | 3022121 |
| [transfer of active oncogenes and promoters into the mouse cell genome]. | different cell dna's (normal nih 3t3 dna; human osteosarcoma cell dna; human malignant glioma cell dna with amplified c-ha-ras) were cotransfected onto nih 3t3 cells with cloned long terminal repeat (ltr) sequences of rous sarcoma virus. ltr rsv and normal nih 3t3 dna c-fos oncogen expression was detected in tumors induced in nude mice. in the same system human tumour cell dna with amplified c-ha-ras gene was used, that to the integration and amplification of ltp sequences with simultaneous main ... | 1986 | 3022122 |
| clinical observations on mechanical ventilation for respiratory failure in bronchiolitis. | an unusually large number of infants (82) were admitted to stanford university hospital from november 1, 1983, through may 31, 1985, with a diagnosis of bronchiolitis requiring oxygen therapy. a larger percentage of these infants (17/82 = 21%) than generally expected required mechanical ventilation for respiratory failure. fourteen infants had respiratory syncytial virus (rsv) infections, and three had parainfluenza virus infections. ten patients had respiratory difficulties as neonates. the mec ... | 1986 | 3022224 |
| synthesis in vitro of a seven amino acid peptide encoded in the leader rna of rous sarcoma virus. | sequences of avian retroviral rnas suggest that short open reading frames in the putatively untranslated leader sequences might direct the synthesis of small peptides. previous analyses indicate that translation of rous sarcoma virus (rsv) rna in vitro faithfully reflects translation of the viral rna in the chick cell. accordingly, we sought to determine if the heptapeptide lp1, encoded in the open reading frame closest to the 5' end of rsv rna, could be synthesized in vitro since this would str ... | 1986 | 3023636 |
| genetic lesions involved in temperature sensitivity of the src gene products of four rous sarcoma virus mutants. | the src genes of four rous sarcoma virus (rsv) mutants temperature-sensitive (ts) for cell transformation were analyzed. the mutant src genes were cloned into a replication-competent rsv expression vector, and the contribution of individual mutations to the ts phenotype was assessed by in vitro recombination with wild-type src sequences. three of the mutants, which were derived from the schmidt-ruppin strain of rsv, each encoded two mutations within the conserved kinase domain. in all three case ... | 1986 | 3023675 |
| noncoding region between the env and src genes of rous sarcoma virus influences splicing efficiency at the src gene 3' splice site. | viral rna and proteins in chicken embryo fibroblasts infected with different cloned variants of the prague strain rous sarcoma virus (rsv) were analyzed. the ratio of immunoprecipitated pp60src to the gag gene product p27 in prague a (pra) and prague b (prb) rsv-infected cells was two to three times that in prague c (prc) rsv-infected cells. a significant increase in the steady-state ratio of spliced 2.7-kilobase src gene mrna to unspliced 9.3-kilobase genome-size rna was observed in pra- and pr ... | 1987 | 3023695 |
| monoclonal antibodies to the v-fes product and to feline leukemia: virus p27 interspecies-specific determinants encoded by feline sarcoma viruses. | monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc poly-protein encoded by snyder-theilen feline sarcoma virus (st-fesv) were prepared. in order to obtain hybridoma clones specific to the antigenic determinants encoded by the fesv genome, lou rats were immunized with st-fesv-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (felv) p27. three distinct clones reactive to both felv p27 and ... | 1986 | 3024406 |
| comparison of the relative importance of tyrosine-specific vinculin phosphorylation and the loss of surface-associated fibronectin in the morphology of cells transformed by rous sarcoma virus. | we have investigated the relative importance of tyrosine-specific phosphorylation of vinculin and the loss of surface-associated fibronectin in the maintenance of the rounded morphology characteristic of chick embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv). to address this question we have examined the interaction of cef and rsv-cef in vitro with exogenously added fibronectin in both 3-day culture experiments and short-term, 3-h spreading experiments. we report that the additio ... | 1986 | 3025229 |
| genetic alterations of integrated avian sarcoma virus dna sequences in transformed rat cells. | clones and subclones of schmidt-ruppin-rsv-d-(srd-) infected rat cells that were isolated in soft agar have shown differences in their morphology. some of the subclones were round or spindle-shaped cells with lower anchorage dependence and high growth rate, while others were more fibroblast-like and failed to overgrow one another. in correlation to their morphology, subclones with high degree of phenotypic transformation contained amplified amounts of full length proviral dna or proviral fragmen ... | 1987 | 3026480 |
| cytoskeletal association of pp60src. the transforming protein of the rous sarcoma virus. | immunoferritin labelling methods have been employed to examine the distribution of the rous sarcoma virus (rsv)-transforming protein pp60src in the detergent-resistant cytoskeleton of transformed cells. pp60src was found to be localized on actin microfilaments present in adhesion plaques, at adherens junctions between cells and also in microfilament bundles. this localization is consistent with the hypothesis that some of the morphological effects of transformation result from the interaction in ... | 1987 | 3026821 |
| comparison of the structural organizations in the 3'-terminal regions of five avian retrovirus strains: rav 7, rav 50, b77, pr-b, and sr-b. | in order to obtain information on the phylogenies of viral strains which belong to rsv (rous sarcoma virus) and alv (avian leukosis virus), the nucleotide sequences of noncoding regions adjacent to the u3 region in two alv strains, rous-associated virus 7 (rav 7) and rav 50, and three rsv strains, bratislava 77 (b77), prague:subgroup b (pr-b), and schmidt-ruppin:subgroup b (sr-b) were determined by extension from a common primer. the sequences thus deduced were compared with known sequences of o ... | 1987 | 3027964 |
| [rhinovirus and acute respiratory infections in infants]. | human leucocytary interferon (ifn) in a concentration of 2,000 ul/ml stops the development of 9 rhinovirus (rv) strains. the use of serum ifn antibodies (saif) reverses this effect. of 256 nasal aspirates culture negative for other respiratory viruses, 40 rv were isolated with saif as opposed to 19 without serum anti ifn. the frequency and some clinical aspects are emphasized in infants: 43% had bronchiolitis similar to respiratory syncytial virus (rsv) infections and atopy was frequent. | 1986 | 3028312 |
| differential transient and long-term expression of dna sequences introduced into t-lymphocyte lines. | we have used a protoplast fusion protocol to introduce the genes encoding neomycin phosphotransferase (neo) and chloramphenicol acetyltransferase (cat) into murine and human t-lymphocyte lines. plasmid constructs containing the neo gene under the control of the promoters from the rous sarcoma virus long terminal repeat (rsv ltr), the sv40 early region, or the herpes simplex virus thymidine kinase gene (hsv tk) can stably transform each of three t-cell lines to g-418 resistance. the characteristi ... | 1986 | 3028736 |
| rous sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interactions. | by immunofluorescence and interference reflection microscopy (irm) we found that f-actin and a group of cytoskeletal proteins involved in microfilament-membrane interaction, including vinculin, alpha-actinin, fimbrin and talin, are specifically organized in discrete dot-like structures corresponding to cell-substratum contact sites in both monocytes and monocyte-derived cells such as macrophages and osteoclasts. these proteins have a precise topological distribution; vinculin and talin form a do ... | 1987 | 3028844 |
| inhibition of methylation at two internal n6-methyladenosine sites caused by gac to gau mutations. | we previously have mapped n6-methyladenosine (m6a) sites within the genomic rna of rous sarcoma virus (rsv). the results of that study and of experiments using inhibitors of methylation suggest that m6a might be involved in mrna processing events. we describe an approach for directly analyzing the function of m6a in rna and for studying the sequence specificity of the m6a methylase. two sites of methylation in rsv (nucleotides 7414 and 7424) were altered by oligonucleotide-directed mutagenesis. ... | 1987 | 3029112 |
| aberrant mhc antigens in a sarcoma virus-induced mouse tumour. | from a series of mouse sarcomata, newly induced by rous sarcoma virus (rsv), the doh cell line was shown to lack expression of syngeneic h-2kd and dk antigens (noll et al., 1986). it unexpectedly displayed determinants specific for h-2kk molecules. interferon treatment of doh stimulated the expression of h-2kk determinants and also the display of some, but not all, determinants of the syngeneic h-2kd molecules. h-2dk expression was not stimulated. southern blot hybridization of genomic dna diges ... | 1986 | 3029231 |
| characterization of rous sarcoma virus sequences essential for viral gene expression. | using the escherichia coli lacz gene product beta-galactosidase as an indicator of gene expression, we analyzed sequences that are required for expression of the rous sarcoma virus (rsv) genome in avian cells. the rsv long terminal repeat (ltr) and leader region were sufficient to direct the synthesis of high levels of enzymatically active gag-lacz fusion proteins. a portion of u3 greater than 140 nucleotides upstream from the cap site was essential for gene expression. this element functioned i ... | 1987 | 3029412 |
| at least two nuclear proteins bind specifically to the rous sarcoma virus long terminal repeat enhancer. | we used the sensitive gel electrophoresis dna-binding assay and dnase i footprinting to detect at least two protein factors (efi and efii) that bound specifically to the rous sarcoma virus (rsv) enhancer in vitro. these factors were differentially extracted from quail cell nuclei, recognized different nucleotide sequences in the u3 region of the rsv long terminal repeat, and appeared to bind preferentially to opposite dna strands as monitored by the dnase i protection assay. the efi- and efii-pr ... | 1987 | 3029568 |
| transformation by rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein. | a cdna clone, designated 9e3, was isolated from a chick embryo fibroblast (cef) cdna library. 9e3 mrna was 20-fold higher in cef following transformation by rous sarcoma virus because of increased transcription rate. in cef infected with temperature-sensitive mutants, increased 9e3 mrna was found within 2 hr of a shift to permissive temperature. nucleotide sequence and in vitro translation results indicate that 9e3 mrna encodes an 11 kd polypeptide that is homologous to human connective tissue a ... | 1987 | 3032449 |
| differentiation of retrovirus-infected avian neuroretina cells. | the effects of oncogenic retroviruses on the expression of differentiation markers were studied in monolayer cultures of chick and quail embryo neuroretinas. transformation by rous sarcoma virus (rsv) did not affect the appearance of synapses, and the expression of glutamic acid decarboxylase was stimulated by pp60v-src, the product of the src gene. quail embryo neuroretina cells transformed by mill hill 2 (which contains the two oncogenes v-mil and v-myc) were induced to proliferate into perman ... | 1987 | 3032462 |
| morphf mutants of rous sarcoma virus: nucleotide sequencing analysis suggests that a class of morphf mutants was generated through splicing of a cryptic intron. | the nature of the lesions involved in producing the fusiform phenotype of three mutants (wo101, wo201, and tsst529) of the schmidt-ruppin a strain of rous sarcoma virus (rsv) was determined by molecular cloning and dna sequencing. wo101 and wo201 contained an in-frame deletion of the v-src region coding for amino acids 116 to 140 of p60v-src. the deleted segment was flanked by consensus splice donor and acceptor sequences and contained an appropriately positioned branchpoint acceptor consensus s ... | 1987 | 3033320 |
| integration and expression of provirus in human cells transformed by avian sarcoma virus. | previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ... | 1987 | 3033382 |
| interferon-gamma inhibits cell replication, but not pp60src activity of rsv-transformed fibroblasts. | the present study analyzes the effect of murine ifn-gamma on dna synthesis, ornithine decarboxylase activity and phosphorylation of pp60src of rouse sarcoma virus transformed cells. when natural or recombinant ifn-gamma was added to quiescent sr-balb or l1210 cells, stimulated with fetal calf serum, only the highest ifn-gamma concentrations (2000 u/ml) inhibited dna synthesis of both tumor lines. by contrast, lower ifn-concentrations (20-200 u/ml) inhibited the dna synthesis of l1210 but not of ... | 1987 | 3033451 |
| rous sarcoma virus is integrated but not expressed in chicken early embryonic cells. | we have developed a protocol that allows us to infect chicken early embryonic (cee) cells with high efficiency. this was achieved by exposing the cee cells to a semicontinuous dose of rous sarcoma virus (rsv) for a period of 20 hr. southern blot analysis indicated that an average of one proviral copy is integrated per embryonic cell. however, there was no production of infectious viral particles by the cells containing the proviral genome, although low levels of full-length genomic rna could be ... | 1987 | 3033663 |
| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| effect of phorbol ester on growth of tumors induced by rous sarcoma virus and on pp60src kinase activity in these tumors. | the inoculation of rous sarcoma virus (rsv) into chickens results in the induction of tumors which usually grow progressively for several weeks and then regress. we have found that the direct injection of phorbol myristate acetate into growing rsv-induced sarcomas resulted in accelerated tumor regression in each of 11 cases studied. we further found that treatment of cultured rous sarcoma cells with phorbol myristate acetate for 72 h resulted in a 50% diminution in numbers of viable cells, in co ... | 1987 | 3036345 |
| haematological and pathomorphological studies on chickens inoculated with transformation-defective sarcoma virus. | a transformation-defective mutant of rous sarcoma virus (td pr-rsv-c) inoculated into chicken embryos caused a number of pathological changes in chickens after hatching. disturbances in haematopoiesis were manifested by anaemia of different degree and by the presence of immature blood elements in the peripheral blood. the haematological picture of erythroblastosis was confirmed histologically. in addition to erythroblastosis, lymphoid leukosis and a proliferative lymphosarcoma-like tumour were d ... | 1987 | 3036610 |
| unstable visible mutations induced in drosophila melanogaster by injections of oncogenic virus dna into the polar plasm of early embryos. | when rsv dna cloned in pbr 322 or dna of simian adenovirus sa7 (c8) is injected into the pole plasm of embryos of various drosophila stocks, the progeny of 1-70% of the surviving flies display visible mutations. the mutagenesis is partially directed: the loci mutating due to retrovirus and adenovirus dna do not overlap. the majority of resulting mutants are characterised by high instability: reversions and new mutations occur in them, which sometimes spread over the whole population ("explosive" ... | 1987 | 3037277 |
| expression of rous sarcoma virus transforming protein pp60v-src in saccharomyces cerevisiae cells. | the rous sarcoma virus (rsv) pp60v-src protein was expressed in saccharomyces cerevisiae cells either from a plasmid vector carrying the v-src gene or in yeast cells containing a single-copy v-src gene chromosomally integrated. in both yeast strains, v-src gene transcription is regulated by the galactose-inducible gal10 promoter. growth in galactose-containing medium resulted in constitutive expression of pp60v-src in the integrated strain and transient expression of higher levels of pp60v-src i ... | 1987 | 3037349 |
| mutants of the rous sarcoma virus envelope glycoprotein that lack the transmembrane anchor and cytoplasmic domains: analysis of intracellular transport and assembly into virions. | the envelope glycoprotein complex of rous sarcoma virus consists of a knoblike, receptor-binding gp85 polypeptide that is linked through disulfide bonds to a membrane-spanning gp37 spike. we used oligonucleotide-directed mutagenesis to assess the role of the hydrophobic transmembrane region and hydrophilic cytoplasmic domain of gp37 in intracellular transport and assembly into virions. early termination codons were introduced on either side of the hydrophobic transmembrane region, and the mutate ... | 1987 | 3041017 |
| respiratory syncytial virus infections and intravenous gamma-globulins. | the respiratory syncytial virus (rsv) is a common cause of bronchiolitis and pneumonia in infants and young children. throughout the world annual rsv epidemics result in numerous hospitalizations, substantial morbidity and some mortality. until the recent introduction of ribavirin only supportive therapy has been available for treating these infections. the development of animal models of rsv infection and the observation that some lots of immunoglobulin prepared for intravenous administration c ... | 1988 | 3041354 |
| respiratory syncytial virus: heterogeneity of subgroup b strains. | in order to investigate further possible structural differences among the two subgroups of respiratory syncytial virus (rsv), we analysed the antigenic characteristics and size of structural proteins of 20 subgroup a and 43 subgroup b strains by their reactions with monoclonal antibodies (mabs) directed against the proteins of rsv using immunofluorescence, elisa and radioimmunoprecipitation assays. the latter test also enabled determination of the size of different structural components. the 37 ... | 1988 | 3045252 |
| passive transfer of respiratory syncytial virus (rsv) antiserum suppresses the immune response to the rsv fusion (f) and large (g) glycoproteins expressed by recombinant vaccinia viruses. | in young infants who possess maternally derived respiratory syncytial virus (rsv) antibodies, the antibody response to rsv glycoproteins is relatively poor, despite extensive replication of rsv. in the present study, it was found that cotton rat rsv hyperimmune antiserum suppressed the antibody response to the rsv glycoproteins but not the response to vaccinia virus antigens when the antiserum was passively transferred to cotton rats prior to infection with vaccinia recombinant viruses expressin ... | 1988 | 3047432 |
| comparison of the abbott and ortho enzyme immunoassays and cell culture for the detection of respiratory syncytial virus in nasopharyngeal specimens. | a comparison of the abbott laboratories and the ortho diagnostic systems respiratory syncytial virus (rsv) enzyme immunoassays (eia) and hep-2 cell culture for the detection of rsv in 81 nasopharyngeal (np) specimens from pediatric patients with lower respiratory tract infection was carried out. the sensitivity and specificity of the abbott test compared to confirmed infection was 92.3% and 100.0%, respectively. the sensitivity and specificity of the ortho test was 87.5% and 80.3%, respectively. ... | 1988 | 3053011 |
| detection, pathogenesis, and therapy of respiratory syncytial virus infections. | respiratory syncytial virus (rsv) infection is a major cause of serious lower respiratory disease in infancy and early childhood. the unique pathogenesis of lower respiratory illness due to rsv offers some intriguing clues to the role of the human immune system in both protection against and development of respiratory illness. more than any other virus, rapid diagnostic techniques have been especially successful in identifying rsv infection. many of these techniques could be easily adaptable to ... | 1988 | 3060243 |
| respiratory syncytial virus. | rsv is now understood to be the most significant viral respiratory pathogen of infants and is capable of causing both bronchiolitis and pneumonia. it is a particular risk to hospitalized infants as the virus is easily spread through close contact. the most vulnerable infants are those who suffer with either congenital heart disease or bronchopulmonary dysplasia who easily fall prey to pulmonary complications of infection. strict environmental control and the use of protective clothing and eyewea ... | 1988 | 3066824 |
| time-resolved fluoroimmunoassay compared with virus isolation for rapid detection of respiratory syncytial virus in nasopharyngeal aspirates. | two monoclonal antibodies against two distinct conserved epitopes of the respiratory syncytial virus (rsv) nucleocapsid protein were used in a direct time-resolved fluoroimmunoassay (tr-fia) for the detection of rsv antigens in nasopharyngeal aspirates. the capture antibody was adsorbed to the solid phase of microdilution strip wells, and the indicator antibody was labeled with a europium chelate. specimens and label were incubated simultaneously for 1 h at 37 degrees c in the coated wells. afte ... | 1988 | 3068251 |
| [bronchiolitis caused by respiratory syncytial virus in very young infants and transmitted maternal antibodies]. | the study with an enzyme linked immunosorbent assay of the sera of 57 infants aged less than 9 weeks and developing a bronchiolitis, argues for the absence of a protective role for the maternally transmitted anti-respiratory syncytial virus (rsv) igg and for the existence of a positive relationship between these antibodies levels - which are not neutralizing - and the severity of rsv bronchiolitis. | 1988 | 3071286 |
| evaluation in non-human primates of the safety, immunogenicity and efficacy of recombinant vaccinia viruses expressing the f or g glycoprotein of respiratory syncytial virus. | it has been shown previously that immunization with recombinant vaccinia viruses expressing the f or g envelope glycoprotein of human respiratory syncytial virus (rsv) strain a2 induced a protective immune response in the lower respiratory tract of cotton rats against live rsv challenge. as a continuation of these studies, the safety, immunogenicity and efficacy of these recombinant vaccinia viruses was evaluated in non-human primates. rhesus and patas monkeys were each inoculated intradermally ... | 1988 | 3072795 |
| [comparison between immunofluorescence and immunoenzymatic assay for the rapid diagnosis of the respiratory syncytial virus in nasopharyngeal secretions]. | an enzyme immunoassay, rsv-eia abbot, was evaluated by comparison with indirect immunofluorescence. nasopharyngeal secretions obtained from 95 infants and young children with acute respiratory infections were examined for the presence of respiratory syncytial virus antigens with both methods. specimens were stored at -70 degrees c before being tested by eia. out of 60 samples positive by indirect immunofluorescence, 46 were also positive by rsv-eia (sensitivity 78.7%) and 34 out of 35 immunofluo ... | 1988 | 3073406 |
| immunological similarity between the insulin receptor and the protein encoded by the src oncogene. | insulin receptors resemble receptors for certain growth factors (epidermal growth factor, platelet-derived growth factor, and insulin-like growth factor i) in that all possess tyrosine-specific protein kinase activity. these cell surface receptors resemble protein kinases encoded by viral oncogenes in that both groups of enzymes phosphorylate proteins on tyrosine. recently, we reported that there is immunological similarity between the insulin receptor and pp60src [the protein encoded by the src ... | 1986 | 3084215 |
| functional analysis of the human tissue-type plasminogen activator protein: the light chain. | a pbr322::rous sarcoma virus(rsv)-based shuttle vector was used to insert fused genes, composed of the amino-terminal portion of the bacterial chloramphenicol-acetyltransferase gene (cat) and the entire coding region for the c-terminally derived light (l) chain of human tissue-type plasminogen activator (t-pa) cdna. cotransfection of rat 3y1 cells with prsvneo dna and prsvcat/t-pa dna yielded stably integrated g418-resistant transfectants which contain unrearranged copies of prsvcat/t-pa dna. th ... | 1986 | 3087818 |
| instability of v-src sequences in nonhuman primate tumors cultured in vitro. | we have analyzed the dna of marmoset tumors induced and marmoset cells transformed by rous sarcoma virus (rsv) and derivative viruses of various types. southern blot hybridization was used to determine the presence of v-src gene sequences. we failed to detect v-src dna in high-passage cells derived from marmoset tumors induced in vivo or from marmoset cell lines transformed in vitro. the inability to detect src sequences was not related to selection of revertants in culture, since all cell lines ... | 1987 | 3111082 |
| association of marek's disease with ea-b and immune response genes in subline and f2 populations of the iowa state s1 leghorn line. | chickens from the iowa state s1 white leghorn line, selected for characteristics of ea-b serotype, humoral immune response to glutamic acid-alanine-tyrosine (ir-gat), and response to rous sarcoma virus (rsv)-induced tumors, were evaluated for genetic resistance to marek's disease (md). in the first two trials, sublines that were triple homozygous for the three traits were challenged with md virus. birds of the b1b1 blood type were significantly (p less than .001) more resistant to md than chicke ... | 1987 | 3112755 |
| expression and glycosylation of the respiratory syncytial virus g protein in saccharomyces cerevisiae. | a cdna encoding the entire amino acid sequence of the g glycoprotein of respiratory syncytial virus (rsv) was inserted into a yeast-escherichia coli shuttle vector such that expression of the virus gene was regulated by the yeast gal1 promoter. transformation of saccharomyces cerevisiae with the vector led to the formation of the g protein when cells were grown in the presence of galactose. under these conditions the rsv g appeared as a 60- to 65-kda glycosylated protein. expression of the g cdn ... | 1987 | 3113070 |
| binding of proteins, including pp60src, to activated ch-sepharose 4b. | activated ch-sepharose 4b and protein a sepharose cl-4b can bind, selectively and non-specifically, polypeptides from chick embryo cells. the major polypeptides bound have apparent molecular masses of 57-60 kda and 47-49 kda and cannot be eluted by extensive washing with buffers containing detergents. one of the 57-60 kda polypeptides was identified by immunoblotting as the transforming protein of rous sarcoma virus (rsv), pp60src. this polypeptide could be removed from the solid phase immunoabs ... | 1987 | 3118186 |
| commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence. | a commercially available direct immunofluorescence (if) test using a reagent consisting of a pool of two monoclonal antibodies against selected surface and internal proteins of respiratory syncytial virus (rsv) was evaluated in comparison with the indirect if technique using a commercial bovine anti-rsv hyperimmune serum for the rapid detection of rsv in nasopharyngeal aspirates from 228 hospitalized children. overall agreement between the two if methods was 95%. the direct if test was quicker t ... | 1988 | 3134216 |
| [isolation and characteristics of antibodies against synthetic fragments of oncoproteins. ii. antiserum against the transforming protein pp60src of rous sarcoma virus]. | to generate the antibodies to the transforming protein of rous sarcoma virus (rsv) pp60src, rabbits were immunized with the peptide, corresponding to 415-421 sequence of pp60src. these antibodies immunoprecipitate pp60src in rsv-transformed chicken and mammalian cells, and also some proteins (45, 85 and 120 kda), which could be autophosphorylated in vitro. it was shown that 415-421 sequence of pp60src is not recognized by the antibodies to pp60src from rsv-induced tumour bearing rabbits (tbr ser ... | 1988 | 3141779 |
| topically administered immunoglobulin reduces pulmonary respiratory syncytial virus shedding in owl monkeys. | a human intravenous immunoglobulin preparation containing neutralizing antibodies to respiratory syncytial virus (rsv) was utilized for intratracheal and topical treatment of laboratory-induced rsv infections in owl monkeys (aotus trivirgatus). topically administered antibodies induced significant reductions in rsv shedding in the lower respiratory tract. | 1988 | 3142351 |
| virus infections in childhood malignant disease. | the viruses isolated during infective episodes over a 5-year period of 93 children with acute lymphoblastic leukemia (all) and 107 children with other malignancies have been studied. viruses were identified by the fluorescent antibody technique (fat), electron microscopy, and culture. viruses were isolated on 204 occasions. rhinovirus and rsv occurred significantly more often in the patients with all, and adenovirus more commonly in the solid tumor patients. viruses were responsible for 4 of the ... | 1987 | 3152935 |
| multiple forms of growth inhibitors secreted from cultured rat liver cells: purification and characterization. | it was found that a non-tumorigenic epithelial cell line from the liver of a buffalo-strain rat (brl) secreted into the culture medium various inhibitors of the growth of brl and rsv-brl (tumorigenic brl transformed by infection of rous sarcoma virus). the secreted inhibitors were classified into two types: one inhibited the growth of brl to a greater extent than that of rsv-brl (non-tumorigenic brl growth inhibitor, ngi), and the other, vice versa (tumorigenic brl growth inhibitor, tgi). two ng ... | 1988 | 3170513 |
| the release of leukotrienes in the respiratory tract during infection with respiratory syncytial virus: role in obstructive airway disease. | samples of nasopharyngeal secretions from a group of 73 infants with bronchiolitis or upper respiratory illness alone during infection with respiratory syncytial virus were analyzed for leukotriene c4 (ltc4) content using a reverse-phase high-pressure liquid chromatography assay with confirmation by radioimmunoassay. titers of respiratory syncytial virus (rsv)-specific ige in nasopharyngeal secretion (nps) specimens were determined using an enzyme-linked immunosorbent assay. the highest concentr ... | 1988 | 3174295 |
| fluorescence and electron microscopic studies of the cytoskeletal organization of normal, established and transformed chick embryo cells. | the cytoskeletons of two established chick embryo cell (cec) lines were examined by fluorescence and electron microscopy and compared with those of control cells and cells transformed by rous sarcoma virus (rsv). in normal cec, many stress fibers were observed. on the other hand, stress fibers were disorganized in nontransformed spontaneously established cec, non-tumorigenic cec partially transformed with a chemical carcinogen, and tumorigenic rsv-transformed cec. in the normal cec, actin filame ... | 1988 | 3177004 |
| studies on the pathogenesis and interspecies transmission of respiratory syncytial virus isolated from sheep. | inoculation of lambs with an ovine isolate of respiratory syncytial virus (rsv) by a combined intranasal and intratracheal route resulted in mild respiratory tract illness, with respiratory tract lesions. lung lesions were characterized by bronchitis and bronchiolitis, hyperplasia of bronchial and bronchiolar epithelium, peribronchiolar and perivascular accumulations of lymphocytes, alveolar septal thickening, and collapse. respiratory syncytial virus was recovered from the respiratory tract of ... | 1988 | 3178037 |
| primary respiratory syncytial virus infection in mice. | a mouse model of respiratory syncytial virus (rsv) infection is described. a high-titered, large-volume inoculum results in replication of rsv to a high titer in lungs of balb/c mice. mice older than 15 weeks of age are more susceptible to rsv infection. titers up to 10(6.9) plaque-forming units (pfu)/gram lung can be attained in 32-week-old mice. older mice experience a clinical illness manifested by ruffled fur, reduced activity, and weight loss. lung histology of older mice infected with rsv ... | 1988 | 3183639 |
| role of respiratory syncytial virus in early hospitalizations for respiratory distress of young infants with cystic fibrosis. | to determine the frequency of respiratory syncytial virus (rsv) as the cause of hospitalization for acute pulmonary exacerbations in young infants with cystic fibrosis (cf), and to assess the clinical effects of rsv infections, we prospectively followed 48 children with a diagnosis of cf after identification by newborn screening. at a mean follow-up age of 28.8 months (range 5 to 59), 18 infants (38%) had been hospitalized a total of 30 times for acute respiratory distress. at the time of admiss ... | 1988 | 3183835 |
| fatal respiratory syncytial virus pneumonitis in a previously healthy child. | respiratory syncytial virus (rsv) is a cause of significant morbidity and mortality in infants and children with an immunocompromised status or a congenital heart disease. the following case describes a 6 8/12-year-old, previously normal child who had a fatal interstitial pneumonitis caused by rsv. documentation of rsv as the etiologic agent and documentation of her immune status are presented. in light of recent advances in the rapid diagnosis and treatment of rsv, this virus should be consider ... | 1988 | 3195838 |
| viruses and airborne allergens as precipitants of obstructive respiratory difficulties in children. | findings of virus infection were prospectively studied in 133 episodes of obstructive respiratory difficulty in 90 children aged 2 to 15 years. a viral infection was confirmed in 16 episodes (12%). in a further 11% of cases serological evidence of a recent viral infection was found. respiratory syncytial virus (rsv) accounted for half of the positive viral results. skin prick tests were performed after the second episode of bronchial obstruction. of the 79 children tested, 57% had at least one p ... | 1988 | 3218914 |
| [antigenic variants of the respiratory syncytial virus in argentina and uruguay]. | the characterization of rsv antigenic variants was carried out on 160 nasopharyngeal aspirates from children under 5 years of age, from argentina and uruguay, with acute respiratory infection and a previously positive etiological diagnosis for rsv. results for argentina were: 20.9% of samples belonged to subtype a, 76.9% to subtype b and the remaining 2.2% to new subtypes as yet uncharacterized. results for uruguay were: 5.6% of samples belonged to subtype a, 81% to subtype b and 13.5% to other ... | 1988 | 3231713 |
| [proliferative activity of normal, transformed and tumor cells in in vitro contact with syrian hamster neutrophils]. | the effect of casein-induced peritoneal neutrophils (nph) of syrian hamsters on the proliferative activity of normal, embryonal, transformed, and tumour cells was determined by their ability to inhibit or to stimulate 3h-thymidine incorporation in the replicating target cells. the enhanced proliferation of normal embryonal cells (he) of hamsters was observed after their in vitro contact with nph, in contrast to the cells of sfhe (spontaneously transformed in vitro he cells of low tumourigenicity ... | 1988 | 3243194 |
| [analysis of respiratory syncytial virus diseases in hospitalized children in the district of steyr 1984/85]. | respiratory syncytial virus (rsv) is the most frequent cause of serious respiratory tract infections in infancy. in the course of a one-year study the nasopharyngeal secretions of all hospitalized children with diseases of the lower respiratory tract (almost exclusively infants) were examined for viral antigens. rsv antigen could be detected in 34 of the 71 secretions. in these infants a serious clinical course, pneumonia, bronchiolitis or obstructive bronchitis was dominant, but laboratory test ... | 1988 | 3260367 |
| cytotoxic t cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus. | we have examined the function of class i mhc-restricted cytotoxic t cells in experimental respiratory syncytial virus (rsv) infection of balb/c mice by transfer of t cell line mjc-a2 and ctl clone e8a into rsv-infected mice. the t cell line cleared pulmonary rsv infection within 5 d in persistently infected gamma-irradiated mice, but caused acute respiratory disease. this was only seen in infected mice and was often lethal after transfer of greater than 3 x 10(6) ctl. lower numbers of ctl produc ... | 1988 | 3262705 |
| seasonal variation in respiratory syncytial virus infections in children in benin-city, nigeria. | the seasonal variation in respiratory syncytial virus (rsv) infections in children was examined in benin city. nasopharyngeal washes were obtained from children under 3 years hospitalised for acute lower respiratory infections during two seasons - rainy season (june-august) and dry season (december-february). rsv surface antigen was identified by elisa. 54% of patients in the rainy season were elisa positive for rsv compared to 8.8% during the dry season. clinical features in the rsv infected pa ... | 1988 | 3265812 |
| detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. | we evaluated prospectively the detection of respiratory syncytial virus (rsv) by culture and by direct antigen detection using an indirect immunofluorescence assay (ifa), a direct monoclonal immunofluorescence assay (dfa), and a monoclonal enzyme immunoassay (eia). of 221 specimens, 95 (43%) were culture positive for rsv, 4 (1.8%) contained more than one virus, and 17 (7.6%) contained a virus other than rsv. overall, hep-2 and flow 6000 cells grew significantly more rsv isolates (82 and 72%, res ... | 1988 | 3281981 |
| detection of an immunoglobulin m response in the elderly for early diagnosis of respiratory syncytial virus infection. | the indirect fluorescent-antibody technique was compared with indirect and mu-capture enzyme-linked immunosorbent assays for the detection of respiratory syncytial virus (rsv) immunoglobulin m (igm) in the elderly. sera from 47 patients (mean age, 70 years) with acute lower respiratory tract infections caused by rsv were investigated. specific igm was detected in 81% (38 of 47) of the patients. the fluorescent-antibody technique, which gave 70% positive results, proved to be the most sensitive o ... | 1988 | 3290237 |
| detection of respiratory syncytial virus antigen in nasopharyngeal secretions by abbott diagnostics enzyme immunoassay. | we compared a rapid respiratory syncytial virus (rsv) antigen enzyme immunoassay (eia) (abbott diagnostics, north chicago, ill.) with virus culture and with the indirect fluorescent-antibody test (fat) by using nasopharyngeal washings from children with suspected rsv pneumonia or bronchiolitis. fresh washings were used in all three tests. specimens were inoculated into hep-2 cells and human embryonic lung fibroblasts and observed for cytopathic effect. cells in the centrifuged sediments of the n ... | 1988 | 3290243 |
| occurrence of respiratory syncytial virus subgroup a and b strains in japan, 1980 to 1987. | the subgroup characteristics of 71 strains of respiratory syncytial virus (rsv) isolated in sapporo, japan, during 5 epidemic years from 1980 to 1987 were determined by the use of 17 monoclonal antibodies (mabs) raised against the rsv long strain, which is now recognized as the prototype subgroup a strain. nine of these mabs immunoprecipitated the fusion protein (f), five immunoprecipitated the large glycoprotein (g), two immunoprecipitated the nucleoprotein (np), and one immunoprecipitated the ... | 1988 | 3290246 |
| evaluation of two commercially available test methods to determine the feasibility of testing for respiratory syncytial virus in a community hospital laboratory. | the feasibility of performing respiratory syncytial virus (rsv) testing in a community hospital laboratory was assessed. the abbott rsv eia kit, an enzyme immunoassay (eia), and the ortho respiratory syncytial virus identification reagent, a direct fluorescent antibody (dfa) technique, were evaluated in terms of technologist time, result time, cost, and agreement of results. there was little difference in the amount of technologist time required for either method. the dfa technique was shown to ... | 1988 | 3293421 |
| comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens. | a total of 211 specimens, including 144 nasopharyngeal aspirates and 67 tracheal secretions, were evaluated for the rapid detection of respiratory syncytial virus (rsv) antigen by three commercial enzyme-linked immunosorbent assays (elisa; kallestad diagnostic, austin, tx; ortho diagnostics, raritan, nj, and abbott laboratories, north chicago, il) and by isolation of rsv in cell culture. of the 61 rsv culture positive specimens, kallestad elisa, ortho elisa, and abbott elisa detected rsv antigen ... | 1988 | 3293894 |
| comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. | paired nasopharyngeal aspirate (npa) and nasopharyngeal swab (nps) specimens obtained from each of 32 hospitalized infants with x-ray-confirmed pneumonia (91%) or bronchiolitis were tested for respiratory syncytial virus (rsv) infection by virus culture, the indirect immunofluorescent-antibody (ifa) technique, enzyme-linked immunosorbent assay (elisa; ortho diagnostic systems, inc.), and spot hybridization with a human genomic probe to quantitate cellular dna. rsv was isolated in cell cultures f ... | 1987 | 3294883 |
| diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. | with the availability of ribavirin therapy for serious respiratory syncytial virus (rsv) infections, rapid diagnostic tests for the detection of rsv antigen are increasingly important. efficacies of a commercially available enzyme immunoassay (eia) (abbott laboratories, north chicago, ill.) and a fluorescent-antibody assay (fa) were evaluated in a study involving 135 specimens from children with respiratory symptoms. a nasal wash specimen was cultured immediately on rsv-sensitive a549 cells; the ... | 1987 | 3294889 |
| rs virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay. | two techniques for rapid diagnosis, immunofluorescence (ifat) and enzyme immunoassay (eia), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (rsv) in specimens of nasopharyngeal secretions. the specimens were obtained from children under five years of age suffering from acute respiratory illness, during a period of six months from january to june 1982. of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) w ... | 1986 | 3295470 |
| clinical review of ribavirin. | the recent approval of ribavirin aerosol for the treatment of severe respiratory syncytial virus (rsv) in infants and young children is a significant addition to the antiviral drugs available today. when administered as an aerosolized form by face mask or mist tent for 20 to 21 hours per day, ribavirin effectively decreases the symptoms of rsv infection and the shedding of rsv virus. studies of other viral infections such as viral hepatitis, influenza a and b, lassa fever, genital herpes, and he ... | 1987 | 3298116 |
| immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus. | the envelope glycoproteins of two distinct strains of respiratory syncytial virus (rsv) (long and 18537 strains) were purified by affinity chromatography and characterized by immunological methods. the fusion (f) proteins from the two strains were similar in molecular weight by gel electrophoresis and were very closely related immunologically. rabbit antisera to either f protein reacted with near equivalent titres with the heterologous f protein by western blot, enzyme-linked immunoassay (eia), ... | 1987 | 3302101 |