| human papillomavirus type 58 dna sequence. | the complete nucleotide sequence of human papillomavirus type 58 (hpv 58) dna cloned from an invasive cervical carcinoma was determined. the hpv 58 genome consists of 7824 nucleotides, containing 37.9% of gc residues, and has a similar genome organization of other hpvs. on the nucleotide sequence level, it conserves the signal sequences for regulation of gene expression as with other genital hpvs and exhibits an extensive homology with hpv 33 (77%). comparative analysis of amino acid sequences r ... | 1991 | 1656594 |
| duplication of the upstream regulatory sequences increases the transformation potential of human papillomavirus type 11. | infections with certain types of papillomaviruses, e.g., human papillomavirus type 16 (hpv-16), often progress to cancer. malignant lesions associated with the closely related hpv-11 are extremely rare. additionally, hpv-11 dna, unlike hpv-16, does not normally transform cells in vitro. we determined that hpv-11 dna was able to transform baby rat kidney cells in a ras-dependent focus assay when the upstream regulatory region (urr) was present in two copies. addition of a second hpv-11 urr or an ... | 1991 | 1656601 |
| detection of human papillomavirus dna in formalin-fixed tissues by in situ hybridization after amplification by polymerase chain reaction. | the authors describe the detection of human papillomavirus (hpv) 16 dna in paraffin-embedded, formalin-fixed tissues of cervical squamous intraepithelial lesions (sils) by in situ hybridization after amplification by the polymerase chain reaction (pcr). using conventional in situ hybridization and a biotin-labeled probe, variable numbers of superficial cells and none of the basal cells in the sils showed detectable hpv 16 dna. when the in situ assay was done after amplification, increased number ... | 1991 | 1656763 |
| human papillomavirus infection of the oral mucosa. | this article reviews the lesions of oral mucosa that contain human papillomavirus (hpv). these hpv-associated lesions can be classified into two broad types on the basis of their biologic behavior, benign lesions and premalignant malignant or malignant lesions. benign oral lesions include squamous cell papilloma (scp), verruca vulgaris (vv), condyloma acuminatum (ca), and focal epithelial hyperplasia (feh). of these entities, vv, ca, and feh demonstrate characteristic hpv-induced cytopathic effe ... | 1991 | 1656809 |
| verruca vulgaris of the larynx. demonstration of human papillomavirus types 6/11 by in situ hybridization. | verruca vulgaris of the larynx (vvl) is a distinctly uncommon lesion related to the human papillomavirus (hpv). the clinical and pathologic features of a case involving the true vocal cords of a 37-year-old woman are presented and compared with the seven cases previously reported in the english language literature. papillomavirus capsid antigen was detected in the excised tissue on immunostaining, and viral particles were seen by electron microscopy. in situ hybridization with biotinylated dna p ... | 1991 | 1656909 |
| the sexually transmitted disease model for cervical cancer: incoherent epidemiologic findings and the role of misclassification of human papillomavirus infection. | the definition of cervical neoplasia as a sexually determined disease caused by some types of human papillomavirus has been widely accepted. recent epidemiologic studies, however, have failed to identify a correlation between sexual activity and human papillomavirus infection. moreover, sexual activity has also been shown to be independent of human papillomavirus infection in increasing cervical cancer risk. these incoherences are analyzed with respect to etiologic models for cervical neoplasia ... | 1991 | 1657209 |
| cloacogenic carcinoma of the anal canal and associated viral lesions. an in situ hybridization study for human papilloma virus. | five cases of cloacogenic carcinoma were analyzed for microscopic human papilloma virus (hpv)-induced changes and with in situ hybridization technique for hpv types 6/11, 16/18 and 31/35/51. four of the five cases showed epithelial foci of koilocytotic atypia. hpv type 16/18 was present in four of the five cases. the surface epithelium in two of the four cases with koilocytotic changes showed hpv type 16/18. hpv type 6/11 was seen in surface epithelium in one case in which invasive carcinoma sho ... | 1991 | 1657356 |
| malignant human papillomavirus type 16-transformed human keratinocytes exhibit altered expression of extracellular matrix glycoproteins. | we found that keratinocytes immortalized with human papillomavirus (hpv) type 16 dna and malignantly converted by h-ras transfection (hpk-1a/ras) exhibit an enhanced ability to synthesize a fibronectin-containing extracellular matrix. gene expression of fibronectin and thrombospondin was increased in tumorigenic keratinocytes compared to control and immortalized keratinocytes, 6- and 9-fold, respectively. increased production of soluble and cell surface-associated fibronectin was not specific fo ... | 1991 | 1657375 |
| degradation of p53 can be targeted by hpv e6 sequences distinct from those required for p53 binding and trans-activation. | human papillomavirus (hpv) types 16 and 18 appear to play a role in the development of ano-genital malignancies, whereas hpv 6 and 11 are usually associated with benign lesions. one hpv16 oncoprotein, e6, complexes with and promotes degradation of the cellular tumor suppressor p53. here we show that e6 proteins of both oncogenic and benign hpv types associate in vitro with p53, but binding by e6 proteins of benign hpv types cannot target p53 for degradation. a c-terminal region of e6 conserved a ... | 1991 | 1657399 |
| human papillomavirus-related diseases of the vulva. | | 1991 | 1657472 |
| identification of human papillomavirus dna in cervical intraepithelial neoplasia by in situ hybridization. | | 1991 | 1657561 |
| the aetiology and surgery of carcinoma of the anus, rectum and sigmoid colon in crohn's disease. negative correlation with human papillomavirus type 16 (hpv 16). | anal and recto-sigmoid crohn's disease may rarely be complicated by the development of local malignancy. eight patients with this association were identified from the records of st. mark's hospital between 1947 and 1988 and two from the london hospital. the aetiology of the malignancies and their surgical management have been examined. eight patients had adenocarcinoma of the anus, rectum or sigmoid colon and two had squamous cell carcinoma of the anus. synchronous dysplasia, adenomas and carcin ... | 1991 | 1657651 |
| evidence that patients presenting with dyskaryotic cervical smears should be screened for genital-tract infections other than human papillomavirus infection. | one hundred and fifty-four women with dyskaryotic cervical smears were referred for colposcopy from two distinct population groups, namely those who initially had attended a sexually transmitted disease (std) clinic, and those who came from general practitioners and family planning and gynaecology clinics (non-std group). they were investigated to determine the prevalence and pattern of concomitant lower genital-tract infections. the mean age in each group was 25 years. microbiological studies r ... | 1991 | 1657654 |
| rapid detection of epstein-barr virus dna in clinical samples of oral hairy leukoplakia with hrp-labeled dna probes and in situ hybridization. | an in situ hybridization technique, using horseradish peroxidase (hrp)-labeled dna probes containing a portion of the epstein-barr virus (ebv) genome, was used to detect ebv dna in tongue sections and smears from patients with oral lesions resembling the clinical features of oral hairy leukoplakia (hl). eleven biopsy specimens (six consistent with hl, four normal tongue controls, and one leukoplakia) and 11 tongue smears were evaluated for the presence of ebv, cytomegalovirus (cmv), herpes simpl ... | 1991 | 1658028 |
| degenerate primers based on highly conserved regions of amino acid sequence in papillomaviruses can be used in a generalized polymerase chain reaction to detect productive human papillomavirus infection. | conserved amino acid sequences within the l1 open reading frame of the human papillomavirus (hpv) genome were used as a basis to design two degenerate primers (gp17 and gp18) and one general probe (gpr22) which direct polymerase chain reaction (pcr) amplification and subsequent detection of a 620 to 660 bp dna fragment. the conserved nature of the primers and probe was tested experimentally on a panel of 24 cloned hpv dnas isolated from cutaneous and mucosal lesions, including hpv-2a and -57, wh ... | 1991 | 1658205 |
| is vestibular papillomatosis associated with human papillomavirus? | the origin and clinical significance of vestibular papillae were evaluated by comparing histological features with the presence of human papillomavirus (hpv) types 6/11 and 16/18, as revealed by southern blot dna hybridization. twenty women with vestibular papillomatosis underwent clinical evaluation and follow-up. when available, male partners were also examined. histological changes suggestive of hpv infection were present in all the 20 specimens. sixteen cases (80%) contained dna sequences ho ... | 1991 | 1658222 |
| cervicovaginal human papillomavirus infection in suburban adolescents and young adults. | to investigate the prevalence rates of and risk factors for genital human papillomavirus (hpv) infection in white, middle-class adolescents, we obtained specimens for hpv from 107 patients undergoing pelvic examination at a suburban adolescent health service. specimens were obtained by means of cervicovaginal lavage and were analyzed for hpv dna by southern blot analysis. the subjects' mean age was 18.5 (+/- 2.0 sd) years; 47% of the subjects were seen for contraception, 36% for possible pregnan ... | 1991 | 1658285 |
| in situ detection of hpv dna in oral mucosal lesions. a comparison of two hybridization kits. | the purpose of this study was to compare the sensitivity of viratype in situ hybridization kit (life technologies, inc. [lt] and pathogene (enzo diagnostics, inc. [ed]) in situ hybridization kit for human papillomavirus (hpv) dna detection in oral tissue. forty benign oral lesions histologically suspicious for hpv infection were analyzed. specimens were hybridized with dna probes specific for hpv types 6/11, 16/18, and 31/33/35 [lt] and hpv types 6/11, 16/18, and 31/33/51 [ed]. positive hybridiz ... | 1991 | 1658315 |
| loss of p53 protein in human papillomavirus type 16 e6-immortalized human mammary epithelial cells. | we have shown previously that introduction of the human papillomavirus type 16 (hpv16) or hpv18 genome into human mammary epithelial cells induces their immortalization. these immortalized cells have reduced growth factor requirements. we report here that transfection with a single hpv16 gene e6 is sufficient to immortalize these cells and reduce their growth factor requirements. the rb protein is normal in these cells, but the p53 protein is sharply reduced, as shown by immunoprecipitation with ... | 1991 | 1658367 |
| tumorigenic transformation of murine keratinocytes by the e5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. | to examine the biological properties of the bovine papillomavirus type 1 (bpv) and human papillomavirus type 16 (hpv16) e5 genes, each was cloned separately into a retroviral expression vector and helper-free recombinant viruses were generated in packaging cell lines. the bpv e5 retroviruses efficiently caused morphologic and tumorigenic transformation of cultured lines of murine fibroblasts, whereas the hpv16 e5 viruses were inactive in these assays. in contrast, infection of the p117 establish ... | 1991 | 1658398 |
| [focal epithelial hyperplasia]. | five cases of "focal epithelial hyperplasia" (feh) of the oral mucosa observed in switzerland are reported. the patients were of turkish and north african extraction. the lesions of feh were multiple, painless, located at various sites of the oral mucosa including the tongue in the form of either soft papules or hard nodules. evidence of a human papilloma virus origin was ascertained. among the 1067 cases reported in the literature and reviewed for this study, this condition has been described t ... | 1991 | 1658583 |
| [diagnosis of cervicovaginal infection by human papillomavirus. value of colposcopy, cytology and histopathology as methods of diagnosis]. | the authors evaluated 476 patients to determine the accuracy of colposcopy, cytology, and histopathology as diagnostic methods for cervical and vaginal hpv infections. results were treated by statistical analysis which showed significant disagreement among the methods tested. colposcopy was positive in 87.8% of the cases. abnormal transformation zone was the most frequent finding in the cervix, acetowhite epithelium being its main representative. condylomatous vaginitis was the most frequent fea ... | 1991 | 1658912 |
| genital human papillomavirus infections in patients attending an inner-city std clinic. | one hundred and sixteen consecutive women attending a baltimore city std clinic were studied for the prevalence of human papillomavirus (hpv) infection of the genital tract using three criteria: presence of clinically recognized (visible) genital warts, cytopathologic evidence suggestive of hpv infection in a papanicolaou smear, and analysis of cervical scrapes for genital tract hpv genomic sequences by southern hybridization. the women were young (median age: 22 years) and more than 80% had a h ... | 1991 | 1658954 |
| vulvovaginal human papillomavirus infections: clinical implications and management. | the past 2 decades have witnessed an alarming increase in the incidence of human papillomavirus infections. clinically evident cases represent only a small portion of the infected population, because millions of people have subclinical or latent infection. human papillomavirus infection is recognized as a precursor to malignancy. thus it is important to treat clinically evident infection. treatment is complicated by the ability of the virus to establish latent infection and the lack of an effect ... | 1991 | 1659197 |
| vulvar vestibulitis syndrome: an overview. | vulvar vestibulitis syndrome is a constellation of symptoms and findings involving and limited to the vulvar vestibule that consists of: (1) severe pain on vestibular touch to attempted vaginal entry, (2) tenderness to pressure localized within the vulvar vestibule, and (3) physical findings confined to vulvar erythema of various degrees. histopathologic findings are consistent with a chronic, nonspecific inflammatory response that is occasionally associated with metaplasia of the minor vestibul ... | 1991 | 1659198 |
| vaginal cancer: the role of infectious and environmental factors. | primary cancers of the vagina are rare. they comprise 1% to 2% of all gynecologic malignancies and occur predominantly in older women. the diagnosis of primary carcinoma of the vagina requires that the cervix and vulva be intact and that no clinical evidence of other primary tumors exist. approximately 90% of all vaginal tumors are squamous cell in type on histologic examination. adenocarcinoma, which is much less common (2% to 4%), is seen primarily in younger women with in utero exposure to di ... | 1991 | 1659200 |
| squamous cell carcinoma of the lung in a nonsmoking, nonirradiated patient with juvenile laryngotracheal papillomatosis. evidence of human papillomavirus-11 dna in both carcinoma and papillomas. | malignant transformation of laryngeal juvenile papillomatosis most often occurs in patients with previous radiation therapy or smoking histories. we report the case of a 35-year-old, nonsmoking, nonirradiated man who developed squamous cell carcinoma of the lung with a 33-year history of laryngotracheal juvenile papillomatosis. postmortem examination showed pulmonary cavitating papillomatosis and chest wall, vertebrae, and peribronchial lymph node involvement by tumor. molecular studies (souther ... | 1991 | 1659237 |
| human papillomavirus and seborrheic keratosis. | | 1991 | 1659247 |
| [detection of human papilloma virus type 16 dna sequences in biopsies of cervical carcinoma]. | | 1991 | 1659483 |
| the clinical role of human papilloma virus typing. | patients with abnormal pap smears underwent repeat pap smear, colposcopy, biopsy, and human papilloma virus (hpv) typing to identify the presence of cin and to assess the correlation of hpv type to grade of cin and behavior of cin. a total of 334 patients underwent evaluation and typing with southern blot methodology. fifty-five percent (185) of the patients demonstrated hpv viral sequences; 98 of the 185 positive patients demonstrated hpv types 16, 18. the presence of hpv sequences was not asso ... | 1991 | 1659553 |
| hpv-16 viral transcripts in vulvar neoplasia: preliminary studies. | specific human papillomavirus (hpv) types are strongly associated with intraepithelial neoplasia and invasive cancer of the cervix. in contrast, the role of hpvs in the pathogenesis of invasive carcinoma of the vulva is poorly understood. we have employed in situ hybridization for the detection of subgenomic transcripts in four vulvar specimens to elucidate the role of hpv type 16 in the development and progression of vulvar cancer. these analyses revealed that the transcripts of the e6-e7 regio ... | 1991 | 1659554 |
| immunocytochemical characterization of large granular lymphocytes in normal cervix and hpv associated disease. | a quantitative immunocytochemical study of large granular lymphocytes (lgls) in the normal cervix and in human papillomavirus (hpv) associated disease was performed using a panel of monoclonal antibodies which included those for lgl surface markers cd56, cd16, and cd57. only cd56-positive cells were found within the ectocervical epithelium and these cells increased in number in cervical intraepithelial neoplasia (cin) in comparison with normal cervix. examination of serial sections and double la ... | 1991 | 1659628 |
| vaginal laser surgery. | vaginal epithelial lesions including endometriosis, cysts, septae, human papillomavirus (hpv) disease, and vaginal intraepithelial neoplasia (vain) can be successfully treated by laser surgery. the carbon dioxide laser with a wavelength of 10,600 nm is the ideal laser for these clinical applications. pretreatment evaluation using colposcopically directed biopsies is mandatory. adherence to surgical techniques including the selection of a delivery system, control of thermal injury, depth of destr ... | 1991 | 1659681 |
| laser treatment of genital human papillomavirus infections in the male patient. | thorough examination of the lower anogenital epithelium of promiscuous men and their partners (either male or female) may be useful to diagnose and, if appropriate, treat those who are likely to perpetuate the hpv epidemics and increase the potential risk for cervical carcinogenesis. co2 laser ablation is a highly attractive means to treat hpv-related disease of the anogenital epithelium in men. the nd:yag laser also has some specific applications in the treatment of large, relatively vascular l ... | 1991 | 1659682 |
| human papillomavirus infection in women with multicentric squamous cell neoplasia. | tissues from 32 women with multicentric squamous cell neoplasia of the anogenital region (72 anatomically distinct lesions at the cervix, vagina, vulva, perineum, or anus) were tested for the presence of human papillomavirus with the polymerase chain reaction or in situ hybridization. all the women had invasive carcinomas or grade 3 intraepithelial neoplasia lesions at a minimum of one site and one or two squamous cell lesions at another site(s). human papillomavirus was detected in all of the m ... | 1991 | 1659785 |
| prevalence of occult human papillomavirus infection, determined by cytology and dna hybridization, in heterosexual men attending a genitourinary medicine clinic. | one hundred and five heterosexual men without evidence of clinical anogenital warts and attending a genitourinary medicine clinic were studied. three separate specimens for cytology were taken from (i) the distal urethra including the perimeatal area, (ii) the penile shaft and glans penis including the sub-preputial area, and (iii) from the anorectal area using a proctoscope. pooled specimens of exfoliated cells from these sites were also taken for the detection of human papillomavirus (hpv) by ... | 1991 | 1659914 |
| systemic alpha-interferon (wellferon) treatment of genital human papillomavirus (hpv) type 6, 11, 16, and 18 infections: double-blind, placebo-controlled trial. | the efficacy of systemic interferon-alpha (ifn-alpha n1; wellferon) treatment on genital human papillomavirus (hpv) infections was studied in a double-blind, placebo-controlled trial. a total of 120 women were randomly allocated to receive either interferon (ifn, n = 60) or placebo (n = 60). in both treatment groups, 15 patients with lesions induced by each of the four hpv types (hpv 6, 11, 16, and 18) were included. the dose of ifn was 1.5 x 10(6) iu subcutaneously three times for the first wee ... | 1991 | 1660011 |
| pcr-detected genital papillomavirus infection: prevalence and association with risk factors for cervical cancer. | in an investigation conducted in student health clinic patients, the polymerase chain reaction was used to detect human papillomavirus (hpv) dna, thereby allowing measurement of the prevalence of hpv infection and study of the association between hpv infection and risk factors for cervical cancer. of 159 women eligible to participate, 105 (66%) provided a specimen of cervical cells for hpv typing, and also answered an interviewer-administered questionnaire which sought information on risk factor ... | 1991 | 1660038 |
| the interaction between hpv infection and estrogen metabolism in cervical carcinogenesis. | cancer of the genital tract is the final outcome of some infections with human papillomavirus (hpvs), and the most estrogen-sensitive cells are at greatest risk for the hpv-related cancers. therefore we investigated relationships between hpvs and estrogen metabolism in cells of the genital tract. increased conversion of estradiol to 16 alpha-hydroxyestrone, known to be a risk factor for cancer in some other estrogen-sensitive cells, was investigated in keratinocytes from the genital tract. prima ... | 1991 | 1660039 |
| [detection of human papillomavirus dna in hypopharyngeal carcinoma]. | the detection of human papillomavirus type-16 and -6b was performed in 28 cases of hypopharyngeal squamous cell carcinomas by polymerase chain reaction. human papillomavirus (hpv) dnas extracted from paraffin-embedded metastatic lymph node tissue were used for polymerase chain reaction with amplification of the e1 region of hpv genotype 6b and the e6 region of hpv genotype 16. hpv type 6b sequences were detected in three cases; positive amplification of type 16 sequences was also seen in three c ... | 1991 | 1660068 |
| human papillomavirus type 16 e6 proteins with glycine substitution for cysteine in the metal-binding motif. | the human papillomavirus type 16 (hpv 16) e6 is a 151 amino acid protein containing four metal-binding motifs, cys-x-x-cys. we constructed and characterized three mutants with gly substitutions for cys within the motif; for cys-66, for cys-136, and for both, respectively. zinc binding to bacterially expressed e6 was markedly reduced by the substitution for cys-66, but dna binding was unaffected by any of these mutations. immunofluorescence staining showed that, whereas the e6 expressed in monkey ... | 1991 | 1660194 |
| identification of the nuclear localization signal of human papillomavirus type 16 l1 protein. | human papillomavirus type 16(hpv16) l1 and l2 capsid proteins can be detected only in the nucleus of infected cells. for other nuclear proteins, specific sequences of basic amino acids(aa) termed nuclear localization signals (nls) direct the protein from the cytoplasm to the nucleus. we used a series of deletion and substitution mutations of the hpv16 l1 protein, produced by recombinant vaccinia virus (rvv), to identify nls within hpv16 l1 and showed that hpv16 l1 contains two nls sequences, eac ... | 1991 | 1660197 |
| analysis of the p53 gene in human uterine carcinoma cell lines. | the inactivation of the tumor suppressor gene p53 has been demonstrated in a variety of human tumors. in this study, we present a p53 gene analysis of 13 uterine carcinoma cell lines. sequencing analysis of the entire coding region revealed mutations changing the p53 amino acid composition in all six endometrial carcinoma cell lines tested (ishikawa, hecl-a, hecl-b, kle, rl95-2, and an-3). of the seven cervical carcinoma cell lines, two (ht-3 and c-33a) contained p53 codon changes as well. we we ... | 1991 | 1660340 |
| verrucous carcinoma of the leg positive for human papillomavirus dna 11 and 18: a case report. | human papillomavirus (hpv) types 6 and/or 11 have been associated with benign lesions, while types 16, 18, 31, and 33 are prevalent in malignant lesions. this case report describes the findings in a verrucous carcinoma of the leg, which was examined for hpv types 11, 16, and 18 by in situ dna hybridization. the lesion gave positive results for hpv subtypes 11 and 18, a combination that, to our knowledge, has not been previously reported in this neoplasm. | 1991 | 1660446 |
| [detection of human papillomavirus types 16, 18 and 33 in exfoliated cervical cells by polymerase chain reaction]. | human papillomavirus (hpv) types 16, 18 and 33 were identified by means of the polymerase chain reaction using exfoliated cells from the uterine cervix in 361 patients. of 261 patients without cervical lesions, 10(3.8%) patients had hpv dna whereas 7(70.0%) of 10 patients with invasive cervical carcinomas had hpv dna. the younger patients' group (29 year-old or less) without cervical lesions had a 6.5% hpv positive rate which was distinctly higher than the older patients' groups. no menopausal p ... | 1991 | 1660508 |
| in-situ hybridization using biotinylated dna probes to human papillomavirus in adenocarcinoma-in-situ and endocervical glandular dysplasia of the uterine cervix. | in-situ hybridization using biotinylated probes to human papillomavirus (hpv) dna was performed on formalin fixed paraffin embedded tissue in 30 patients with histologically confirmed adenocarcinoma-in-situ (ais). thirteen of the 30 cases contained areas of endocervical glandular dysplasia (egd) admixed with ais. twenty one patients showed positive staining of the ais nuclei for hpv dna. ten cases (33%) were positive for hpv 16 dna and 11 cases (37%) were positive for hpv 18 dna. no case showed ... | 1991 | 1660591 |
| the histopathology of biopsies taken from women attending a new zealand colposcopy clinic. | analysis of the histopathology of biopsies taken from 1371 women attending the dunedin hospital colposcopy clinic during the period 1982-1988 showed that cin3, either alone or in conjunction with human papillomavirus (hpv) infection, was the most commonly encountered abnormality. women under the age of 29 years accounted for 58.3% of the cin3 lesions. there was also a dramatic increase in the number of women with evidence of hpv infection. several cases of invasive carcinoma were encountered, 3 ... | 1991 | 1660592 |
| human papillomavirus type 6 detected by the polymerase chain reaction in invasive sinonasal papillary squamous cell carcinoma. | eight sinonasal carcinomas (one adenocarcinoma, two undifferentiated nasopharyngeal carcinomas, and five squamous cell carcinomas) were investigated for evidence of human papillomavirus (hpv) infection using in situ hybridization and the polymerase chain reaction for hpv types 6, 11, 16, 18, and 33. all eight cases were negative for hpv infection by in situ hybridization, while a single hpv-6-positive case was identified by the polymerase chain reaction. the hpv-positive case was an invasive pap ... | 1991 | 1660705 |
| laryngeal papillomatosis with human papillomavirus dna contracted by a laser surgeon. | a 44-year-old laser surgeon presented with laryngeal papillomatosis. in situ dna hybridization of tissue from these tumors revealed human papillomavirus dna types 6 and 11. past history revealed that the surgeon had given laser therapy to patients with anogenital condylomas, which are known to harbor the same viral types. these findings suggest that the papillomas in our patient may have been caused by inhaled virus particles present in the laser plume. | 1991 | 1660719 |
| the hygromycin-resistance-encoding gene as a selection marker for vaccinia virus recombinants. | hygromycin b (hy), an inhibitor of rna translation, was shown to block the replication of vaccinia virus (vv) in cultured cell lines. insertion of the escherichia coli hy resistance-encoding gene (hph) into the vv genome under control of early or late synthetic vv promoters could overcome inhibition of viral replication. when hph was inserted into vv in tandem with the human papillomavirus type 16 (hpv16) l1 open reading frame, hph recombinant viruses could be selected which expressed hpv16 l1. | 1991 | 1660832 |
| human papillomavirus and c-myc/c-erbb2 in uterine and vulvar lesions. | the aim of this study was to investigate papillomavirus (hpv)-dna in precancer and cancer of the cervix, vulva, and endometrium by in situ/dot blot/southern blot hybridization and polymerase chain reaction (pcr). myc/erbb-2 expression was examined by northern blot analysis. pcr was the most sensitive hpv detection method, demonstrating hpv-dna in all pre-invasive and invasive cervical lesions (n = 21) and most (3 of 4) vulvar carcinomas in contrast to an overall rate of 60% with other techniques ... | 1991 | 1661047 |
| testing for human papillomavirus: basic pathobiology of infection, methodologies, and implications for clinical use. | new molecular biological evidence has recently emerged, strongly implicating the human papillomavirus (hpv) as playing an etiologic role in the development of neoplasias of the genital tract. as technologies advance, the ability to test for the presence of hpv has become simpler, more reliable, and less expensive. a great deal of controversy has arisen regarding the effective and proper utilization of these new tests in the management of hpv infections. this review will detail the new evidence i ... | 1991 | 1661053 |
| prospective serial study of viral change in the cervix and correlation with human papillomavirus genome status. | to observe in serial fashion the histological changes of human papilloma virus (hpv) infection of the cervix and to correlate these with the detection of hpv genomes. | 1991 | 1661138 |
| human papillomavirus-associated anogenital neoplasia and other solid tumors in human immunodeficiency virus-infected individuals. | the incidence and variety of solid tumors reported among human immunodeficiency virus (hiv)-infected individuals are increasing. among the most common of these tumors are anogenital malignant and premalignant tumors associated with human papillomavirus infection. cervical intraepithelial neoplasia is one such human papillomavirus-associated lesion and appears to be more common among women with hiv infection than hiv-negative women. cervical intraepithelial neoplasia also appears to progress more ... | 1991 | 1661170 |
| transformation of rat-embryo immortalized fibroblasts by the e6-e7 region of human papillomavirus type 18. | plasmids containing the e6 and e7 open reading frames of human papillomavirus type 18 transformed rat-embryo fibroblasts when expressed under the cytomegalovirus promoter. the fibroblasts had been previously immortalized with the large t-antigen gene of the polyomavirus to produce rat embryo fibroblast (large t-antigen) [ref(lt)] cells. ref(lt) cells were transformed by the e6 and e7 sequences to anchorage independence and tumourigenicity, but there were no significant morphological alterations. ... | 1991 | 1661187 |
| the association between pregnancy and human papilloma virus prevalence. | this study examined the effects of pregnancy on the prevalence of hpv infection, comparing 69 pregnant and 54 nonpregnant age-frequency matched female patients. hpv prevalence was detected by dna hybridization using the virapap/viratype dot blot procedure. the prevalence of hpv among pregnant women increased with gestational age from 8.0% in the first trimester, to 16.7% in the second, and 23.1% in the third trimester. this finding suggests that hpv infection may be activated by hormonal or othe ... | 1991 | 1661203 |
| human papillomaviruses and small cell carcinoma of the uterine cervix. | the in vitro dna amplification technique of polymerase chain reaction was used to evaluate the possible presence of human papillomavirus (hpv) in small cell carcinoma of the uterine cervix. none of the 12 cases examined contain detectable amounts of either hpv type 16, 18, 31, or 33 dna. on the other hand, hpv types 16 and 18 dna were found in 14 (93.3%) and 9 (60.0%) of 25 invasive cervical squamous carcinoma tissues. the results seem to suggest that these types of hpv are not present or are pr ... | 1991 | 1661261 |
| hpv changes and their significance in patients with invasive squamous cell carcinoma of the vulva: a clinicopathologic study. | records of 28 patients with invasive squamous cell carcinoma of the vulva were analyzed with regard to age-specific incidence rate, associated human papillomavirus (hpv) changes, multifocal and unifocal distribution of the lesions, and incidence of nodal metastasis. the presence of hpv changes (koilocytosis and condyloma) around the neoplastic epithelium correlated with a mean age group younger than that of those without hpv changes (47 vs 77 years). all multifocal cancers were associated with h ... | 1991 | 1661262 |
| genital-type human papillomavirus infection is not associated with surface epithelial ovarian carcinoma. | tumor tissues from 29 women with borderline or malignant epithelial ovarian tumors were examined for the presence of human papillomavirus (hpv) dna by the polymerase chain reaction (pcr). the pcr analysis used a set of consensus primers that are complementary to highly conserved sequences in the genital hpvs (m. m. manos, y. ting, d. k. wright, a. j. lewis, t. r. broker, and s. m. wolinsky, cancer cells 7, 209-214, 1989). amplification products were detected by southern hybridization with consen ... | 1991 | 1661263 |
| correlation of histology and detection of human papillomavirus dna in vulvar cancers. | the purpose of this study was to correlate the histological findings and clinical information for vulvar precancers and cancers with the detection of human papillomavirus (hpv). forty-five squamous cell lesions (twenty-two high-grade squamous intraepithelial lesions (sils) and twenty-three invasive cancers) were analyzed for hpv dna after amplification by the polymerase chain reaction. hpv dna was noted in 13/22 (59%) high-grade sils and 7/23 (30%) invasive cancers. for the invasive cancers, hpv ... | 1991 | 1661264 |
| presence of human papillomavirus type 18 dna in a pharyngeal and a laryngeal carcinoma. | we examined the presence of human papillomavirus (hpv) genome in pharyngeal and laryngeal squamous cell carcinomas by employing the polymerase chain reaction. we detected hpv 16 dna in one of 11 pharyngeal carcinomas and in 3 of 28 laryngeal carcinomas, and hpv 18 dna in a pharyngeal and a laryngeal carcinoma. in one of the laryngeal carcinomas, dna of both hpv types 16 and 18 were detected. to our knowledge, this is the first report of detection of hpv type 18 in head and neck carcinomas. | 1991 | 1661280 |
| detection of human papillomavirus type 16 in sexual partners of patients having cervical cancer by polymerase chain reaction. | the polymerase chain reaction (pcr) was employed to detect human papillomavirus (hpv) 16 and 18 in cytological samples from the uterine cervix and in urine samples from the male consorts. hpv 16 was detected in 2 (25%) of 8 males whose wives were positive for hpv 16, while it was detected in only one (7%) of 14 consorts whose wives were negative for hpv 16 and 18. this is the first report of the detection of hpv 16 in urine. viral detection in urine samples by the pcr method is a non-invasive, c ... | 1991 | 1661281 |
| a cellular protein mediates association of p53 with the e6 oncoprotein of human papillomavirus types 16 or 18. | the e6 protein of human papillomavirus types 16 and 18 (hpv-16 and hpv-18) can stably associate with the p53 protein in vitro. in the presence of rabbit reticulocyte lysate, this association leads to the specific degradation of p53 through the ubiquitin-dependent proteolysis system. we have examined the e6-p53 complex in more detail and have found that association of e6 with p53 is mediated by an additional cellular factor. this factor is present in rabbit reticulocyte lysate, primary human kera ... | 1991 | 1661671 |
| benign human papillomavirus infection in renal transplant recipients. | warts and cutaneous squamous cell carcinomas are common complications of immunosuppression. we studied a total of 189 renal transplant recipients clinically for such lesions. the incidence of warts increased steadily after transplant, such that of patients transplanted for more than 5 years, 92% were found to have warts and 65% had more than five warts each. dna extracted from scrapings of their warts showed they carried the same human papillomavirus types as the general population, and not the ... | 1991 | 1661712 |
| human papillomavirus types and recurrent cervical warts in immunocompromised women. | little is known about the host's immunological response to human papillomavirus (hpv) infection, which is an important co-factor in genital tract cancers. this study analyzed the hpv type in pre- and posttreatment tissues in immunocompromised women with cervical squamous intraepithelial lesions (sils) that recurred after ablative therapy. the hpv type was the same in the pre- and posttreatment lesion in nine of 12 recurrent sils in women immunocompromised because of aids or long-term immunosuppr ... | 1991 | 1661899 |
| [mild cervix dysplasia: does diagnostic biopsy have a "therapeutic" effect?]. | the aim of the study was, to evaluate the possible curative effect of punch biopsy on low grade cervical intraepithelial dysplasia (cin i) as indicated by the literature. after a three-month interval, 115 women with pap-smears indicating cin i were subsequently controlled by cytologic cervical smears. additional colposcopically directed punch biopsies were taken in 26 patients at the first examination. the remission rate was similar in patients with or without punch biopsy (54% to 49%), as well ... | 1991 | 1662168 |
| human papillomavirus 6, 11, and 16 in laryngeal papillomas. | twenty-seven cases of benign laryngeal papillomas, both single and multiple variants, were analysed for human papillomavirus (hpv) by dna slot-blot hybridization chiefly to determine the pattern of infection in hong kong chinese. dna was extracted from paraffin blocks of formalin-fixed tissue and probed separately for hpv 6, 11, 16, and 18. sixteen cases (59 per cent) showed the presence of at least one of these four hpv genomes. thirteen cases (48 per cent) were positive for hpv 11 only. three ... | 1991 | 1662265 |
| in vitro modulation of human laryngeal papilloma cell differentiation by retinoic acid. | we have defined conditions permitting the in vitro growth of human laryngeal papilloma cells at the air-liquid interface. using this model system, retinoic acid has been found to modulate the differentiation of human laryngeal papilloma cells along two different pathways. at low concentrations of retinoic acid [10(-9) mol/l and 10(-8) mol/l], the cells formed a stratified squamous epithelium with a differentiation-specific protein staining pattern identical to that found in vivo. at higher conce ... | 1991 | 1662346 |
| human papillomavirus 16 e7 protein is associated with the nuclear matrix. | the cellular localization of the human papillomavirus (hpv)-16 e7 gene product in the cell lines caski and siha has been determined by both biochemical and immunocytochemical methods. these measurements show e7 to be localized in the cell nucleus, specifically with the nonchromatin nuclear structure or nuclear matrix. this localization of e7 required an unambiguous fractionation of the nuclear constituents. this was achieved by using a gentle sequential fractionation procedure to prepare the sca ... | 1991 | 1662388 |
| baculovirus expression of the human papillomavirus type 16 capsid proteins: detection of l1-l2 protein complexes. | the human papillomavirus (hpv) type 16 major capsid proteins l1 and l2 have been produced in a baculovirus expression system. both proteins are expressed at a high level and can be readily solubilized. the l1 capsid protein migrates close to its expected mr of 60k. on the other hand l2 exhibits a much higher mr migrating at 73k, which is considerably greater than its predicted mr of 50k. the identity of both proteins has been confirmed also by western blot analysis. both proteins are produced in ... | 1991 | 1662690 |
| unique topography of dna-protein interactions in the non-coding region of epidermodysplasia verruciformis-associated human papillomaviruses. | the non-coding region (ncr) of the epidermodysplasia verruciformis (ev)-associated human papillomavirus 8 (hpv-8) has been investigated for sequence-specific dna-protein interactions with nuclear proteins from epithelial hela cells. using dnase i protection analysis 18 footprints could be found within the hpv-8 ncr, covering altogether over 60% of both dna strands. several footprints coincided with the known binding sites of transcription factors (nf1, ap1, octamer and pea3 consensus sequences); ... | 1991 | 1662691 |
| a transformation-specific polypeptide distinct from heat shock proteins is induced by herpes simplex virus type 2 infection. | a tumour-specific polypeptide designated u90 is one of a set of polypeptides which are encoded by the host cell and are specific for the transformed cell state, being immunoprecipitated by the sera of tumour-bearing animals. the interest in these tumour-specific polypeptides centres on the finding that they are also recognized by antisera raised against herpes simplex virus type 2 (hsv-2)-infected cells, implying some role for hsv-2 in tumorigenesis. the peptide map of hsv-2-induced u90 is indis ... | 1991 | 1662699 |
| detection of human papillomavirus in cervical swabs from indian women by cytological and immunocytochemical technique. | cervical swabs were collected from 88 women in the age group of 17-55 years in calcutta, india to study the prevalence of human papillomavirus (hpv) infection. the viral infection was demonstrated on the basis of koilocytotic changes in papanicolaou staining (pap test) and the presence of viral capsid antigen as detected by immunocytochemistry (imc test). the pap and imc tests revealed 25% and 28.4% of women, respectively, to be positive for hpv infection. higher frequency (61.1% to 87.5%) of in ... | 1991 | 1662781 |
| structural analysis of the human papillomavirus type 16-e2 transactivator with antipeptide antibodies reveals a high mobility region linking the transactivation and the dna-binding domains. | in order to probe the structure of the transcription factor encoded by the e2 open reading frame of papillomaviruses, we raised polyclonal antibodies against a series of synthetic peptides that cover the hpv16-e2 protein. in gel shift experiments with the native form of the protein, we detected supershifts (caused by the binding of antibodies to the e2-dna complex) with antibodies synthesized against peptides covering a central region 50 residues long in the e2 protein. on the contrary, antibodi ... | 1991 | 1662805 |
| virapap: can it help to reduce the need for colposcopy? | two new tests recently became available for identifying cervical human papillomavirus (hpv). this study investigated the usefulness of the virapap and viratype in the clinical setting. thirty-six subjects considered to be at risk for hpv infection were examined; had specimens obtained for papanicolaou smear (pap smear), virapap, and viratype; and were then referred for colposcopy. results of these examinations were then compared to the findings of colposcopy and/or colposcopic biopsy. the sensit ... | 1991 | 1662859 |
| cervical intraepithelial neoplasia and human papilloma virus infection. | evidence of human papilloma virus (hpv) infection was sought in 50 patients with cervical intraepithelial neoplasia (cin) and 50 controls. cytologic, colposcopic and histopathological examinations were carried out in each one of them. cytological evidence of hpv infection was found in 13 cases and two controls. colposcopic evidence was found in 33 (66%) cases and 17 (34%) controls and histopathological evidence in 35 (70%) patients and 14 (28%) controls. these differences were statistically sign ... | 1991 | 1663073 |
| detection of human papillomavirus and human cytomegalovirus in cervical lesions by in situ hybridization using biotinylated probes. | infections with specific types of human papillomavirus (hpv) have emerged as necessary but not sufficient factors in the development of the majority of cervical cancers. the infection by human cytomegalovirus (hcmv) has also been implicated in both cervical intraepithelial neoplasia (cin) and cancer. in order to test prevalence of these viral pathogens in genital lesions with suspect cytopathic changes after observation of smears, cervical biopsies from 131 patients were obtained under colposcop ... | 1991 | 1663402 |
| senescence as a mode of tumor suppression. | two independent lines of experimental evidence are presented in support of the hypothesis that senescence is a normal mechanism of tumor suppression, a homeostatic device designed through evolution to limit cell proliferation irreversibly and thereby to protect the organism against cancer. one set of experiments uses normal human foreskin fibroblasts, transfected at early passage with sv40 dna and subsequently infected with the k-ras virus. if the cells are immortal prior to infection, they beco ... | 1991 | 1663451 |
| treatment of a patient with vulvar vestibulitis by intramuscular interferon beta; a case report. | a patient with severe dyspareunia caused by vulvar vestibulitis, associated with human papillomavirus infection, was treated by intramuscular interferon beta. the treatment was well tolerated and no side effects were reported. a complete resolution of the vestibulitis was noticed 2 months later. no recurrence was noted during a year of follow-up. | 1991 | 1663464 |
| cervical neoplasia and human papilloma virus infection in prostitutes. | to evaluate the prevalence and incidence of pap smears indicating cervical dysplasia as well as human papillomavirus (hpv) infection in prostitutes. | 1991 | 1663486 |
| genital human papillomavirus lesions of the male sexual partners: the diagnostic accuracy of peniscopy. | | 1991 | 1663487 |
| characterization of human papillomavirus type 66 from an invasive carcinoma of the uterine cervix. | human papillomavirus (hpv) dna sequences coexisting with hpv16 and hpv45 were cloned from an invasive cervical carcinoma. the cloned hpv was shown to be a novel type, named hpv66, and is related to hpv56 (an hpv detected in cervical cancer). after screening 160 anogenital biopsies, four specimens exhibited histological features of intraepithelial neoplasia and contained hpv66 sequences. of these, three were found to be associated with another hpv type. | 1991 | 1663515 |
| sequence variants of human papillomavirus type 16 in clinical samples permit verification and extension of epidemiological studies and construction of a phylogenetic tree. | genomic variability between different viral isolates provides a powerful epidemiological tool for verifying ultrasensitive diagnostic procedures, understanding infectious pathways in individuals and human populations, and studying viral evolution. the potential of this approach has not yet been exploited for the diagnosis of human papillomaviruses (hpvs) like hpv type 16 (hpv-16), which are involved in genital cancer. toward this end, we amplified by polymerase chain reaction, cloned, and sequen ... | 1991 | 1663516 |
| structures of bovine and human papillomaviruses. analysis by cryoelectron microscopy and three-dimensional image reconstruction. | the structures of bovine papillomavirus type 1 (bpv-1) and human papillomavirus type 1 (hpv-1) were determined at 2.5 nm resolution by cryoelectron microscopy and three dimensional image reconstruction techniques. as expected, the reconstructions showed that both viruses consist of a t = 7 icosahedral capsid (approximately 60 nm in diameter) which surrounds a nucleohistone core. the capsid morphologies of the two viruses are nearly indistinguishable. each capsid consists of a shell layer (approx ... | 1991 | 1663794 |
| tumorigenic transformation of primary rat embryonal fibroblasts by human papillomavirus type 8 e7 gene in collaboration with the activated h-ras gene. | particular types of human papillomavirus (hpv) are associated with skin cancer of epidermodysplasia verruciformis (ev) patients. here, we show, for the first time, that the e7 gene of ev-associated hpv8 possesses a potential oncogenic transforming ability. the hpv8 e7 open reading frame (orf) and the hpv16 e7 orf were cloned under the sv40 promoter/enhancer to construct recombinant plasmids pcd2-8e7 and pcd2-16e7, respectively. transfection of primary rat embryonal fibroblasts having an activate ... | 1991 | 1663918 |
| detection of human antibody against the human papillomavirus type 16 e7 protein. | we examined 500 human sera for the presence of antibody against the human papillomavirus type 16 e7 protein by enzyme-linked immunosorbent assay with bacterially expressed fusion protein lac-e7, and by radioimmunoprecipitation and immunofluorescence assays with the e7 protein expressed in monkey cos-1 cells. the anti-e7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted ... | 1991 | 1663920 |
| [tumor viruses in urology with special respect to human papillomavirus and human polyomavirus]. | the advent of molecular biology has made a great progress for hpv research. it is now considered that more than 90% of cervical carcinomas are caused by hpv infection. however, it is the problem that penile lesions associated with cancer-related hpv types are subclinical, resulting in acceleration of hpv transmission by male reservoir. we urologists should make an effort to treat hpv infection and to prevent hpv prevalence. on the other hand, any pathological role of jcv has not been revealed in ... | 1991 | 1664005 |
| human papillomavirus infection of the female genital tract. | | 1991 | 1664261 |
| comparison of four non-radioactive and 35s-based methods for the detection of human papillomavirus dna by in situ hybridization. | human papillomavirus dna was detected in 40 condylomatous lesions of various sites (vulva, cervix, larynx, penis and anus) by in situ hybridization using 35s-labelled probes and four non-radioactive probes to compare the various sensitivities of these techniques on the same material (formalin-fixed and paraffin-embedded sections). radioactive probes yielded 28 positive results out of 40 (70%). sulphonated probes (hybricyte kit) also gave 28 positive results with a fine pattern of hybridization g ... | 1991 | 1664430 |
| hpv in full thickness cervical biopsies: high prevalence in cin 2 and cin 3 detected by a sensitive pcr method. | a new type-specific, sensitive, non-radioactive assay is described for the detection of human papillomavirus (hpv) dna in tissues. sequences within the e6 gene were amplified by the polymerase chain reaction (pcr), using primer pairs which clearly distinguish hpv types, including those with close sequence homology such as 6b and 11. the amplified dna products were identified by non-radioactive oligonucleotide hybridization and restriction endonuclease mapping, and the method was sufficiently sen ... | 1991 | 1664460 |
| the clinicopathological significance of cytomegalovirus inclusions demonstrated by endocervical biopsy. | a healthy 20 yr old woman presented for evaluation following a cervical smear which showed viral effects typical of human papilloma virus. colposcopy showed changes of cervicitis with the main finding on histologic examination of biopsy material being an acute and chronic cervicitis associated with typical features of cytomegalovirus (cmv) infection. viral identification was confirmed by immunoperoxidase staining, in situ hybridization and electron microscopy. the patient was lost to follow up f ... | 1991 | 1664515 |
| deleterious effects of formalin/acetic acid/alcohol (faa) fixation on the detection of hpv dna by in situ hybridization and the polymerase chain reaction. | as part of a study of archival cervical cancer specimens (1920s to 1980s) to determine whether changes have occurred in the prevalence of human papillomavirus (hpv) dnas, investigations were performed on tissues which had been fixed in either 10% buffered formalin (nbf) or formalin-acetic acid-alcohol (faa). hpv dna was detected by in situ hybridization (ish) using hpv 6, 11, 16 and 18 32p-labelled dna probes under conditions of high stringency; and by the polymerase chain reaction (pcr) using 2 ... | 1991 | 1664516 |
| [the status of human papillomavirus 16 dna in the tissues of human esophagus carcinoma]. | dnas from 24 cases of human esophagus carcinoma and 24 cases of corresponding para-carcinoma tissues were detected by dot hybridization and southern hybridization techniques using human papillomavirus (hpv) 16 type dna as probe. the results indicated that the positive rate of the hpv 16 dna hybridization was 50% (12/24) in esophagus carcinoma tissues, and 37% (9/24) in para-carcinoma tissues. the results of southern hybridization showed obviously the positive hybridizing band in the zone of 7.2 ... | 1991 | 1664814 |
| [cytometric profile of the dna content in pathologies of the uterine cervix]. | through flow cytometry, we have analysed dna content of cervical cells recovered by scrapping the uterine cervix in 1) 103 women without human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin), 2) 42 patients with hpv infection without cin, and 45 patients with cin. we have observed four different dna patterns: 1) normal dna pattern, 2) increased and heterogeneous dna pattern (ih), 3) increased s g2 + m phases, and 4) dna aneuploid pattern. statistical calculation has e ... | 1991 | 1665092 |
| [cervix dysplasia and human papillomavirus infection in prostitutes]. | | 1991 | 1665134 |
| [intracutaneous test for detection of cellular immune reactions to hpv-16 proteins in patients with cin]. | | 1991 | 1665135 |
| episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation. | it was postulated that non-isotopic in situ hybridisation (nish) signal types 1-3 for human papillomavirus in cervical biopsy specimens represent episomal or integrated virus. the aim of this study was to validate this hypothesis by independent molecular techniques. fresh cervical intraepithelial neoplasia (cin) and squamous cell cancer (scc) tissue were examined for nish signal pattern by hybridising with digoxigenin labelled hpv 16. dna was extracted from the same samples and analysed by restr ... | 1991 | 1665153 |
| the role of human papillomavirus in carcinoma. | human papillomaviruses have long been identified as the cause of common warts and condylomata acuminata. evidence is now accumulating that human papillomaviruses may be involved in not only benign, but also in malignant neoplasms. they might also be significant in the malignant transformation of cutaneous neoplasms. this review examines the oncogenic potential of human papillomaviruses when they are found in carcinomas in various anatomic areas, including the skin. | 1991 | 1665155 |
| [typing of papillomaviruses in cervical dysplasias. its value in treatment]. | the typing fo human papillomavirus (hpv) was carried out in cervical lesions in order to decide on the best therapy to carry out in mild and medium dysplasias of the cervix. 131 patients who had an iodo-negative zone or a smear suggesting an hpv infection had microbiopsies carried out under colposcopic control. every lesion had two biopsies carried out side by side to study the histopathology and the virology. dysplastic lesions were found in 93 biopsies. a search for the dna of hpv 6a, 16 and 1 ... | 1991 | 1665161 |
| cold coagulation therapy in the treatment of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix. | the efficacy of cold coagulation therapy in the treatment of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix, was studied among 65 patients who also had genital warts. this therapy was found to be successful as the failure rate was only 8%. there were no serious side-effects. the treatment can be administered easily in an out-patient setting after histological confirmation. this study also showed that if, on the other hand, a subclinical hpv infection is ... | 1991 | 1665340 |