| role of early genes in pathogenesis of adenovirus pneumonia. | intranasal inoculation of type 5 adenovirus into the cotton rat sigmodon hispidus produces a pneumonia pathologically similar to that in humans, and it, therefore, provides an excellent animal model to investigate the pathogenesis of this disease. the goal of this study was to test the hypothesis that accumulation of viral structural proteins is responsible for a major portion of the cell-damage-producing disease. since viral dna replication is essential for synthesis of the viral structural pro ... | 1990 | 2166948 |
| disseminated adenovirus (type 19) infection in a neonate. rapid detection of the infection by immunofluorescence. | a case of fatal disseminated adenovirus infection in a neonate who suffered from severe keratoconjunctivitis and pneumonitis is reported. the diagnosis was made seven days after the onset of illness based on the detection of adenovirus antigen in the smears of the tracheal suction and conjunctival swab by immunofluorescence. viral antigen was detected in the frozen or formalin-fixed autopsy specimens of the lungs, kidneys, spleen, liver and lymph nodes. typical crystal arrangement of adenovirus ... | 1990 | 2166992 |
| evidence for the multistep nature of in vitro human epithelial cell carcinogenesis. | in keeping with the multistep development of human cancer in vivo, a stepwise approach to neoplastic transformation in vitro presents a reasonable strategy. we have recently developed an in vitro multistep model suitable for the study of human epithelial cell carcinogenesis. upon infection with the adenovirus 12-simian virus 40 hybrid virus, primary human epidermal keratinocytes acquired an indefinite life span in culture but did not undergo malignant conversion. subsequent addition of kirsten m ... | 1990 | 2167163 |
| the expression of the adenovirus 12 early region 1b 19k protein using a recombinant simian virus 40 system. | using a simian virus 40/adenovirus 12 (ad12)-recombinant virus, the ad12 early region 1b (e1b) 19k protein has been produced at high levels after infection of cos 1 cells. expression of the 19k polypeptide reaches a maximum at about 48 h post-infection, declining at later times as host cell death occurs. using two-dimensional isoelectric focusing/sds-polyacrylamide gel electrophoresis, we have shown that the ad12 e1b protein is a major species following infection of cos 1 cells with recombinant ... | 1990 | 2167925 |
| high level expression in 293 cells of the herpes simplex virus type 2 ribonucleotide reductase subunit 2 using an adenovirus vector. | the herpes simplex viruses (hsv-1 and hsv-2) encode a ribonucleotide reductase consisting of two non-identical subunits (rr1 and rr2) which associate to form the active holoenzyme. to facilitate the purification and subsequent biochemical characterization of this enzyme, we have cloned the small subunit 2 of the hsv-2 ribonucleotide reductase (rr2hsv-2) in a helper-independent adenovirus type 5 vector under the control of the adenovirus type 2 major late promoter. after infection of 293 cells wi ... | 1990 | 2167932 |
| effect of human adenovirus on the ellipsoid-associated cells of the chicken's spleen. | ellipsoid-associated cell (eac) responded in two ways to the intravenously injected human adenovirus. the eac may produce a chemotactic factor(s) which recruited heterophils to the periellipsoid white pulp. this early event was shown to occur immediately after the virus injection. within 6 h of injection, the eac disappeared from the ellipsoid and their vacated sites appeared edemic which suggested that the eac may be the target cell of the adenovirus in the chicken spleen. | 1990 | 2168547 |
| serological surveillance for antibodies against different avian infectious agents on turkey flocks naturally infected with turkey rhinotracheitis. | turkey rhinotracheitis (trt) in the state of baden-württemberg, germany appears to have become endemic affecting all farms and every new restocking. in 5 meat turkey flocks, serological surveillance for antibodies to trt-, ib- and ibd-viruses, as well as to pasteurella multocida were carried out using elisa tests. furthermore, sera were examined for the presence of antibodies to avian adenovirus (celo virus), reo- and paramyxo-viruses 1 and 3. the birds were bled at 4 week intervals starting at ... | 1990 | 2168612 |
| [a strategy for immortalizing lines committed to endoderm, neuroectoderm or mesoderm from mouse teratocarcinoma]. | with the aim of immortalizing embryonic cells fixed at early embryonic stages, various plasmids carrying the sv40 early region were introduced into the mouse embryonal carcinomas (ec) f9 and 1003. only the construction pk4, in which the sv40 oncogenes are placed under the control of the adenovirus e1a promoter, led to the immortalization of the cells at the onset of differentiation. clones corresponding to committed precursors of each embryonic lineage (neuroectoderm, mesoderm and endoderm) were ... | 1990 | 2168713 |
| cytopathology of adenovirus keratitis by replica technique. | corneal replicas were made from the severely affected eyes of 12 patients presenting with typical signs and symptoms of epidemic keratoconjunctivitis. adenovirus was isolated from the eyes. histopathological study of the replicas and cytology of diseased cells removed with the replica showed diffuse mild oedema of the epithelium, with scattered moderately swollen and deformed cells. the clinically observed punctate lesions histologically consisted of markedly swollen cells that had become oval o ... | 1990 | 2171632 |
| construction of a defective adenovirus vector expressing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine. | the gene encoding the pseudorabies virus glycoprotein gp50 was cloned at the very left end of the genome of adenovirus type 5 to give a recombinant adenovirus (ad-gp50) defective for the e1a gene. ad-gp50 expressed high levels of gp50 in cells which either complemented (293 cells) or did not complement (vero and hela cells) the e1a gene. surprisingly, over an extended period, higher levels of gp50 were produced in hela cells which lack the e1a gene. rabbits and mice inoculated with ad-gp50 showe ... | 1990 | 2172456 |
| further characterization of an avian adenovirus associated with inclusion body hepatitis in bobwhite quails. | an adenovirus (isolate 1452) associated with inclusion body hepatitis of bobwhite quails (colinus virginianus) was characterized as a group i, serotype 1 avian adenovirus and was indistinguishable from quail bronchitis virus. bobwhite quails were inoculated via the intratracheal or intraperitoneal route with 10(6) mean tissue-culture infective dose of isolate 1452 at 1, 3, 6, or 9 weeks of age. lesions produced by either route of inoculation were similar to those of quail bronchitis and included ... | 1990 | 2173532 |
| failure to detect viral genomic sequences of three viruses (herpes simplex, simian virus 40 and adenovirus) in human and rat brain tumors. | little is known about oncogenesis in brain tumors. viruses are thought to be involved in some neurological diseases, the presence of subfractions of viral dna has been reported in various circumstances and the oncogenicity of some viruses has been demonstrated in animal experiments. the discovery of homologies between retroviral oncogenes and normal cellular genes (proto-oncogenes) has stimulated once again the search for viral responsibility in oncogenesis. having a large bank of tumor material ... | 1990 | 2174028 |
| growth of avian adeno-associated virus in chicken cells transfected with fowl adenovirus serotype 1 dna. | using the chloroquine-modified calcium phosphate coprecipitation technique, fowl adenovirus serotype 1 (fav 1) dna transfects efficiently chicken cell cultures. infection of fav 1 dna transfected cells with helper dependent avian adeno-associated virus (aaav) results in the production of aaav progeny, being detected by an indirect immunofluorescence assay. these findings indicate that fav 1 dna introduced into the host cell promotes actively the growth of aaav. | 1990 | 2176225 |
| genotoxicity and cell cycle effects of platinum-based imaging agents in cisplatin-resistant human tumour cells. | a series of cis-dichloroplatinum(ii) 2,3-diaminopropionamide complexes synthesised as potential imaging agents was tested for activity against a human ovarian tumour cell line (ci-80-13s) with high natural resistance to cisplatin and carboplatin as compared with other human cells. the most potent compound, the dimethyl ester of dichloro-[4-(methyleneiminodiacetic acid)phenyl (2',3'-diamino-propionamide)]platinum(ii) (complex iii), exhibited toxicity towards ci-80-13s cells similar to that observ ... | 1990 | 2182213 |
| high level expression of the envelope glycoproteins of the human immunodeficiency virus type i in presence of rev gene using helper-independent adenovirus type 7 recombinants. | the effect of rev (art/trs) gene on the level of hiv-1 envelope (env) expression using recombinant adenovirus was investigated. recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (hiv-1) were constructed by inserting the gene into an expression cassette. the expression cassette contained the adenovirus type 7 major late promoter, followed by leader 1 of the adenovirus tripartite leader and a portion of intron between leaders 1 and 2 ... | 1990 | 2183470 |
| protein synthesis initiation factor modifications during viral infections: implications for translational control. | infection of tissue culture cells with certain viruses results in the shutoff of host cell protein synthesis. we have examined virally infected cell lysates using two-dimensional gel electrophoresis and immunoblotting to ascertain whether initiation factor protein modifications are correlated with translational repression. moderate increases in eukaryotic initiation factor (eif)-2 alpha phosphorylation are detected in reovirus- and adenovirus-infected cells, as reported previously (samuel et al. ... | 1990 | 2188834 |
| adenovirus proteinases: comparison of amino acid sequences and expression of the cloned cdna in escherichia coli. | adenoviruses (ad) synthesize serine-center endoproteinases (adeps) responsible for maturation cleavages within the virus particle. many questions regarding these enzymes remain unanswered because previous studies utilized crude cells or viral lysates as the enzyme source. here, we report on the comparison of the amino acid (aa) sequences of several adeps and on the expression of the cdna of the ad2ep in escherichia coli. the adeps consist of about 200 aa and their size is around 23 kda. among th ... | 1990 | 2189788 |
| epidemiological analysis of acute respiratory disease (ard) and characteristics of the influenza epidemic in bohemia during the season 1986/1987. | the authors analyze the findings of epidemiological and virological surveillance of ard in bohemia during the season 1986/1987. in all, 57.5% of the czech population was affected by acute respiratory disease (ard). there were 5,950,832 cases reported, 124,444 complications (2.1% of the overall morbidity rate) and 5,374 deaths due to influenza, bronchitis, pneumonia and chronic pulmonary affection. the influenza epidemic commenced during the 48-th calendary week (cw) and lasted 5 weeks till the 5 ... | 1990 | 2212637 |
| adenovirus type 2 precursor proteins are cleaved by proteinases of other adenoviruses. | a virus-encoded endoproteinase is responsible for the cleavage of adenovirus type 2 (ad2) precursor proteins. by analyzing the genetic maps of previously characterized interserotypic recombinants between ad2 and ad5 and by means of an in vitro proteinase assay using ad1, 2, 3, 4, 5, 6, 7, 9 as the source of enzyme, it was demonstrated that (i) the enzyme was present in these serotypes and by extension it is likely to be present in all adenoviruses, and (ii) that proteolytic cleavage can take pla ... | 1990 | 2219735 |
| the upstream factor-binding site is not essential for activation of transcription from the adenovirus major late promoter. | an adenovirus major late promoter (mlp) has been constructed with a 4-bp alteration in the sequence which binds the transcription factor known as usf or mltf. this upstream element has often been considered necessary and sufficient for maximal transcription of the mlp. a duplex oligonucleotide containing the mutant sequence was not capable of binding specific proteins in a band shift assay, nor was it capable of inhibiting such binding by the wild-type sequence. in an in vitro assay, the mutant ... | 1990 | 2243379 |
| potential use of live viral and bacterial vectors for vaccines. who meeting, geneva, 19-22 june, 1989. | the use of vaccinia virus vector for the delivery of antigens was first described by moss and paoletti and their colleagues in 1982. such vaccines could be of particular value in developing countries because they would be cheap, stable, easy to administer and provide long-lasting immunity. who recognized the potential value of such a delivery system by convening two meetings, one at the national institutes of health, usa in november 1984 and the second at who headquarters, geneva, switzerland in ... | 1990 | 2251869 |
| a protein serologically and functionally related to the group c e3 14,700-kilodalton protein is found in multiple adenovirus serotypes. | a 14.7-kilodalton protein (14.7k protein) encoded by the e3 region of group c adenoviruses has been shown to protect virus-infected fibroblasts from lysis by tumor necrosis factor (tnf) (l.r. gooding, l.w. elmore, a.e. tollefson, h.a. brady, and w.s.m. wold, cell 53:341-346, 1988). in this study we show that adenoviruses of other groups are also protected from tnf-induced cytolysis. representative serotypes of groups a, b, d, and e produce a protein analogous to the 14.7k protein found in human ... | 1990 | 2304142 |
| purification and functional characterization of adenovirus ts111a dna-binding protein. fluorescence studies of protein-nucleic acid binding. | the adenovirus single-stranded dna (ssdna)-binding protein (dbp) is necessary for the elongation step in viral dna replication. in an attempt to characterize the putative ssdna-binding domain of the dbp, we purified and characterized the ad2ts111a dbp, which contains a glycine-to-valine substitution at amino acid 280. this mutation is adjacent to that in the previously studied ad2+nd1ts23. ad2+nd1ts23 exhibits a temperature-sensitive defect in dna replication, and its dbp has previously been sho ... | 1990 | 2318838 |
| extracts of hamster cells abortively infected with human adenovirus type 12 are competent to support initiation of viral dna replication. | baby hamster kidney (bhk-21) cells do not allow replication of human adenovirus type 12 (ad12) dna during abortive infection by this virus. however, we have determined that crude extracts of bhk-21 cells abortively infected with ad12 support in vitro the initiation reaction of ad12 dna replication. synthesis of the ad12 ptp-dcmp initiation complex by bhk extracts is two- to five-fold less than when crude infected human (kb) cell extracts are used in the reaction. combining infected kb cytoplasmi ... | 1990 | 2330676 |
| physical mapping of two temperature-sensitive adenovirus mutants affected in the dna polymerase and dna binding protein. | we have determined the exact nature of two thermosensitive (ts) adenovirus mutants, h5ts19 and h5ts149, which map to different genes in the e2 transcription unit. the h5ts19 mutation appears to stem from a single base-pair change of a-t to g-c at position 1840 (numbering as in ref. 1), corresponding to codon 154 of the gene coding for dbp. this results in a glutamine-to-arginine change in the amino-terminal domain of the protein. h5ts19 is defective in a late stage of infection, during virus ass ... | 1990 | 2392826 |
| retrovirus-mediated transfer of an adenovirus gene encoding an integral membrane protein is sufficient to down regulate the receptor for epidermal growth factor. | we have used retrovirus-mediated gene transfer to introduce sequences encoding a 10,400-molecular-weight (10.4k) adenovirus protein previously shown to down regulate the receptor for epidermal growth factor (egf) into two murine cell lines that possess human egf receptors (egf-rs). assays for receptor expression showed that acute infection resulted in rapid, constitutive down regulation of the egf-r via a pathway that appears to be endosome mediated. this represents the first demonstration that ... | 1990 | 2398898 |
| adenovirus and minute virus of mice dnas are localized at the nuclear periphery. | the localization of adenovirus 2 (ad2) and minute virus of mice (mvm) dnas was studied in situ in infected hela cell nuclei using fluorescent dna probes and confocal microscopy. ad2 dna was found in multiple foci which were localized along the periphery of the infected cell nuclei. mvm dna was found in hela cell nucleoli which are associated with the nuclear envelope, and when co-infected with ad2 mvm dna was compartmentalized to multiple foci which again were localized at the nuclear periphery. ... | 1990 | 2408013 |
| the use of polylysine during negative staining of viral suspensions. | the use of 0.1% aqueous solution of polylysine (poly-l-lysine) is proposed as a prior step to negative staining of viral or particle suspensions. particles spread better on films precoated with polylysine than with other substances used for the same purpose. this applies particularly to samples from sucrose or cscl gradients. the use of negative contrast substances is discussed. | 1985 | 2409100 |
| immunoenzymatic staining of viral and chlamydial antigens in cell culture. | methods are described for the conjugation of antibodies with biotin and for the use of these reagents in an immunoperoxidase staining procedure for infected cell cultures. this technique provides a simple, rapid, and specific approach to the identification and characterization of a number of viral and chlamydial isolates in the diagnostic laboratory. | 1985 | 2411466 |
| a small nuclear ribonucleoprotein associates with the aauaaa polyadenylation signal in vitro. | rnas containing the polyadenylation sites for adenovirus l3 or e2a mrna or for sv40 early or late mrna are substrates for cleavage and poly(a) addition in an extract of hela cell nuclei. when polyadenylation reactions are probed with ribonuclease t1 and antibodies directed against either the sm protein determinant or the trimethylguanosine cap structure at the 5' end of u rnas in small nuclear ribonucleoproteins, rna fragments containing the aauaaa polyadenylation signal are immunoprecipitated. ... | 1986 | 2423249 |
| analogous amino acid sequences in myelin proteolipid and viral proteins. | computer analysis of the intrinsic membrane protein, myelin proteolipid, shows strong sequence similarities between the putative extramembrane segments of the proteolipid protein and a number of viral proteins, several of which infect humans. these similarities are even more striking than those reported previously between viral proteins and the encephalitogenic myelin basic protein (mbp). these findings, along with other reports of molecular mimicry by viruses, suggest that immunological cross-r ... | 1986 | 2429866 |
| innate immunity during equid herpesvirus 1 (ehv-1) infection. | intrinsic phagocytosis and killing of c. albicans by equine monocytes and polymorphonuclear leucocytes (pmn) was examined during equid herpesvirus 1 (ehv-1) (subtypes 1 or 2) and adenovirus infections. monocyte function increased during ehv-1 subtype 2 and adenovirus infection. conversely, there was an impairment of monocyte ingestion during ehv-1 subtype 1 infection which was ascribed to virus replication in peripheral blood mononuclear cells. pmn phagocytosis was not decreased in any of the in ... | 1986 | 2431815 |
| the role of interferon in the adenovirus-induced augmentation of granulocyte-mediated cytotoxicity in chicken. | the effects of human adenoviruses on the granulocyte-mediated natural cytotoxicity of chicken leukocytes were investigated. a significant, but transient augmentation of granulocyte cytotoxicity was observed 24 h after virus injection, followed by a relatively long period of its suppression. a good correlation was found between the augmented cytotoxicity and interferon induction. the interferon-inducing capacity of adenovirus type 6 and type 12 in vitro similarly ran parallel with their ability t ... | 1987 | 2439443 |
| analysis of antiviral resistance in the intestinal tracts of nude mice infected with a mouse adenovirus. | upon intestinal infection of heterozygous (nu/+) mice with mouse adenovirus, antiviral resistance can be seen in the form of negative-recovery of virus from feces 2-3 wks post infection. on the other hand, in similar infections in balb/c nude (nu/nu) mice, the antiviral resistance is manifested as negative-recovery of virus from feces at a later stage, i.e., at around 6 wks post infection. this resistance was not accompanied by rises in interferon titer, rises in natural killer (nk) cell activit ... | 1987 | 2453086 |
| evidence for two points of restriction in the expression of adenovirus type 2 in cultured epidermal keratinocytes. | cultures of epidermal keratinocytes contain two populations of cells, a basal undifferentiated population and a suprabasal terminally differentiated population. when exposed to wild-type adenovirus type 2 (wtad2), the suprabasal cells are positive by immunofluorescence for capsid antigen and exhibit cytopathic effects (cpe) (r.f. laporta, and l.b. taichman, virology 110:137-146, 1981). the basal cells, although infected, are not positive for capsid antigen and do not display cpe. despite cpe and ... | 1988 | 2459413 |
| characterization of group ii avian adenoviruses with a panel of monoclonal antibodies. | the interaction between a panel of ten monoclonal antibodies and hemorrhagic enteritis virus, a group ii avian adenovirus, was determined. the monoclonal antibodies reacted with all nine isolates of group ii avian adenoviruses, but not with any of five types of group i avian adenoviruses. all ten monoclonal antibodies recognized antigenic determinants on the hexon protein of hemorrhagic enteritis virus when analyzed by immunoprecipitation and immunoblotting. they reacted only with the native hex ... | 1988 | 2461793 |
| biological concepts in virus classification. | | 1988 | 2461915 |
| [dynamics of interferon formation in acute viral respiratory infections in children]. | | 1988 | 2462207 |
| promoter-dependent transcription by rna polymerase ii using immobilized enzyme complexes. | dna fragments containing the adenovirus 2 major late or simian virus 40 early promoters were attached to a solid support via a biotin-streptavidin linkage at one end of the fragment, upstream of the rna start site. templates immobilized in this manner were incubated with hela cell nuclear extracts to form preinitiation complexes containing rna polymerase ii and accessory proteins required for faithful in vitro transcription. these complexes did not require atp or datp for assembly, were sensitiv ... | 1989 | 2464595 |
| multiple transcription terminators in e1a and e1b genes of adenovirus type 5. | we located and characterized transcription terminators in the e1a and e1b genes by transferring the 3' fragments of the genes into the vector pscat10 [sato et al., mol. cell. biol. 6 (1986) 1032-1043] at a site located immediately downstream from the cat gene (coding for chloramphenicol acetyltransferase; cat) and upstream from the simian virus 40 polyadenylation region. multiple terminators were located downstream from the e1b gene, but not in the 3' region of the e1a gene. fine analysis of the ... | 1988 | 2465206 |
| transfection of fetal rat intestinal epithelial cells by viral oncogenes: establishment and characterization of the e1a-immortalized slc-11 cell line. | intestinal epithelial cells from 19-day-old rat fetuses underwent electropermeabilization and were successfully transfected by three recombinant plasmids containing the cloned oncogenes from the human adenovirus type 2 early region e1a (slc-11 cells) and polyoma virus and simian virus 40 large t tumor antigens (slc-21 and slc-41 cells). slc-11 cells were propagated for 21 months in culture (current passage, 76; doubling time, 17 hr) and were immortalized by e1a, as shown by rna transfer blot (no ... | 1989 | 2470094 |
| [nucleolar apparatus function and the ribosomal gene activity of mouse embryonic fibroblasts infected by the sa7(c8) virus]. | computer morphometry revealed statistically significant (p less than 0.001) decrease in the number and area of nucleoli and ribosomal gene activities (as demonstrated by agnor staining) in primary mouse embryonal fibroblast cultures infected by oncogenic adenovirus sa7(c8). even more drastic increased in these parameters was found when serum-depleted cultures were infected. there were no effects on nucleolar and agnor parameters in mock-infected cultures. it is suggested that an increase in the ... | 1989 | 2472264 |
| [the antigenic relationship of human and bovine adenoviruses studied by the fluorescent antibody method]. | | 1986 | 2478870 |
| comparison of human and monkey cells for the ability to attenuate transcripts that begin at the adenovirus major late promoter. | late transcription from the adenovirus major late promoter can terminate prematurely at a site 182 to 188 nucleotides downstream. experiments have been designed, with run-on transcription in nuclei in vitro or riboprobe protection of rna obtained both in vivo and in vitro, that demonstrate that the ratio of attenuator rna to readthrough rna is greater in monkey cells (cv-1) than in human cells (hela). this may explain, in part, why the human adenoviruses replicate more poorly in cv-1 cells than ... | 1989 | 2503624 |
| [inserted mutations induced in the yellow gene of drosophila melanogaster by microinjection of oncogenic virus dna into the polar plasma of early embryos]. | | 1989 | 2509178 |
| functions of the two adenovirus early e1a proteins and their conserved domains in cell cycle alteration, actin reorganization, and gene activation in rat cells. | rat embryo cells were infected with adenovirus type 5 mutants that code for only one of the two early e1a proteins, mutants with defects in one of the two conserved regions common to the two proteins, or mutants with defects in the 46-amino-acid region unique to the 289-amino-acid e1a protein. cells were scored for altered cell cycle progression, disruption of actin stress fibers, and activation of e2a expression. mutants lacking either e1a protein were able to cause all of these effects; but mu ... | 1989 | 2521185 |
| a block in mammalian splicing occurring after formation of large complexes containing u1, u2, u4, u5, and u6 small nuclear ribonucleoproteins. | the assembly of mammalian pre-mrnas into large 50s to 60s complexes, or spliceosomes, containing small nuclear ribonucleoproteins (snrnps) leads to the production of splicing intermediates, 5' exon and lariat-3' exon, and the subsequent production of spliced products. influenza virus ns1 mrna, which encodes a virus-specific protein, is spliced in infected cells to form another viral mrna (the ns2 mrna), such that the ratio of unspliced to spliced mrna is 10 to 1. ns1 mrna was not detectably spli ... | 1989 | 2522588 |
| genome structure of adenovirus 12 host range mutants adapted to growth in simian vero cells. | mutants of adenovirus type 12 adapted to growth in vero cells were studied by restriction enzyme and sequence analysis. plaque-purified mutants were isolated either after long-term passage of the virus in vero cells (cs-1, cl-1) or from vero cells transfected with viral dna (11-g, 12-k). the plaque-forming activity of these mutants was equal or even slightly higher in vero cells than in hela cells, whereas the activity of wild-type virus in hela cells exceeded that in vero cells by about one to ... | 1989 | 2524454 |
| cytotoxicity of gelonin conjugated to targeting molecules: effects of weak amines, monensin, adenovirus, and adenoviral capsid proteins penton, hexon, and fiber. | it has been reported previously that ammonium chloride, chloroquine, monensin, and adenovirus-2 potentiate the cytotoxicity of several protein toxins conjugated with various targeting molecules. we have tested whether these agents, and protein components of adenovirus-2, would enhance the cytotoxicity of conjugates of gelonin with j5, an antibody directed against common acute lymphoblastic leukemia-associated antigen, with 5e9, an antibody directed against human transferrin receptor, or with ric ... | 1989 | 2531272 |
| the adenovirus early region 4 open reading frame 6/7 protein regulates the dna binding activity of the cellular transcription factor, e2f, through a direct complex. | nuclear factor e2f is a cellular protein that binds to the adenovirus e2 promoter and e1a enhancer regions and to the cellular c-myc p2 promoter region. the dna binding activity of e2f, detected in vitro using nuclear extracts prepared from hela cells, is increased by adenovirus infection (termed e2f induction). we demonstrate here that a 19.5-kd protein, encoded by adenovirus early region 4 (e4) open reading frame (orf) 6/7, is primarily responsible for the induction of e2f dna binding activity ... | 1989 | 2532611 |
| adenovirus region e3 proteins that prevent cytolysis by cytotoxic t cells and tumor necrosis factor. | human adenoviruses are providing insights into strategies that viruses may adopt to evade immune surveillance. there are 47 serotypes that form six groups (a to f) with different genetic and biological properties. adenovirus type 2 (ad2) and ad5, two group c types, the most common and best understood in terms of molecular biology, cause respiratory infections in young children and often form persistent infections. following infection, the linear duplex dna genome is expressed in two broad phases ... | 1989 | 2533958 |
| effect of adrenal blocking chemicals on viral and respiratory infections of chickens. | in a series of experiments chickens were treated with chemicals which block the production of corticosterone by the adrenal cortex prior to being challenged with respiratory disease (and other) agents in order to determine if the course of the diseases could be altered. some chickens received a single intramuscular injection (14 mg/kg) of 1,1-dichloro-2,2-bis/p-chlorophenyl/ethane (abc) dissolved in corn oil (20 mg/ml) at least 12 h before challenge. other chickens received feed containing 500 m ... | 1989 | 2536580 |
| replication of adeno-associated virus in cells irradiated with uv light at 254 nm. | irradiation of simian virus 40 (ori mutant)-transformed chinese hamster embryo cells (od4 line) with uv light induced a cellular capacity which supported a full cycle of helper-independent adeno-associated virus replication. monochromatic uv light at 254 nm was about 1,000-fold more effective than uv light at 313 nm, indicating that cellular nucleic acid is the primary chromophore in the uv-induced process leading to permissiveness for adeno-associated virus replication. the uv irradiation and t ... | 1989 | 2536816 |
| neonatal adenovirus pneumonia. | a 13-day-old premature infant with hyaline membrane disease was found to have altered pneumocytes, suggesting viral infection. pneumocytes had nuclear inclusions that were basophilic or eosinophilic with poorly preserved cytoplasm. monoclonal antibodies specific for the nucleocapsid of herpes simplex virus types 1 and 2 applied to formalin-fixed lung sections were nonreactive. electron microscopy of lung tissue showed the characteristic crystalline arrays of adenovirus. documented adenovirus inf ... | 1989 | 2537069 |
| clinical and laboratory findings in children with adenovirus infections. | the hospital records of 49 children with adenovirus infection were reviewed. diagnosis was made by virus isolation from the airways in 73%, the stools in 44%, the conjunctiva in 2% and by serology in 14% of the patients. most children were less than 3 years of age. the peak incidence of virus isolation occurred during the month of april. pharyngotonsillitis was the most frequent main diagnosis (49%), followed by pneumonia (14%), gastroenteritis (10%) and bronchitis (8%). fever was the most frequ ... | 1989 | 2537728 |
| embryonal neuroepithelial tumors induced by human adenovirus type 12 in rodents. 1. tumor induction in the peripheral nervous system. | human adenovirus type 12 (ad 12) was inoculated intraperitoneally, intrapleurally, intramuscularly or subcutaneously into newborn rodents. tumors developed preferentially in the peritoneal cavities in 93.9% of the hamsters and 82.6% of the mice, but none in rats; in contrast to the high incidence of brain tumors in rats when the virus is injected intracranially. serial section of peritoneal tissues and muscle of hamsters revealed multicentric microtumors with a close relation to peripheral nerve ... | 1989 | 2538040 |
| detection of latent avian adenovirus-associated virus proteins in chicken cells. | chicken kidney cells, derived from the eggs of white leghorn chickens that had serological evidence of prior exposure to both adenovirus and the adeno-associated virus (aav), produced aav antigenic proteins upon challenge with purified adenovirus. antigen was detected by indirect immunofluorescence using monoclonal antibody to aav. the number of fluorescent cells were few and did not increase during the course of adenovirus infection. similar results were obtained using cells prepared from speci ... | 1989 | 2539071 |
| nontranslated cellular mrnas are associated with the cytoskeletal framework in influenza virus or adenovirus infected cells. | in an effort to understand the molecular mechanisms underlying the selective shutoff of host protein synthesis in influenza virus and adenovirus infected cells, we analyzed the subcellular location of representative cellular and viral mrnas. earlier work has shown that the majority of cellular mrnas remain polysome associated after infection by either virus and that both the initiation and elongation steps of host protein synthesis were blocked in infected cells (m. g. katze, d. decorato, and r. ... | 1989 | 2539695 |
| management and prevention of ocular viral and chlamydial infections. | a majority of cases of preventable and/or curable ocular morbidity and blindness are caused by ocular infections. they may account for 70 to 90% of all ocular morbidity seen by family doctors, general practitioners, health centers, and local ophthalmologists in both developed and developing countries. unfortunately, most health authorities and doctors, including ophthalmologists, consider these diseases to be of little or no importance because they are not fully aware of the high prevalence of t ... | 1989 | 2539947 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |
| adenoviral fibrinous enteritis in calves. | two calves coming from different herds showed at post-mortem examination a fibrinous enteritis with mucosal necrosis particularly in the ileal-colonic tract. histologically, amphophilic intranuclear inclusion bodies were the main feature. they were located in the endothelial cells of the intestine and mesenteric lymph cells. ultramicroscopic examination of the inclusion bodies revealed the presence of an adenovirus. in one case the virus was isolated on cell cultures and further classified as bo ... | 1989 | 2540945 |
| in vitro formation of short rna polymerase ii transcripts that terminate within the hiv-1 and hiv-2 promoter-proximal downstream regions. | trans-activation of hiv-1 transcription by the viral regulatory protein tat has been proposed recently to overcome a block to rna polymerase ii elongation in vivo imposed by 5'-untranslated leader sequences. interestingly, however, only full-length transcripts, rather than prematurely terminated hiv rnas, are synthesized in most cell-free transcription extracts. here, we describe an in vitro system in which induction of a highly efficient rna polymerase ii termination or cotranscriptional rna pr ... | 1989 | 2542124 |
| overlapping octamer and taatgarat motifs in the vf65-response elements in herpes simplex virus immediate-early promoters represent independent binding sites for cellular nuclear factor iii. | expression of the immediate-early (ie) genes of herpes simplex virus (hsv) is specifically stimulated by a 65-kilodalton virion transcription factor (vf65 or vp16) that is introduced as a component of infecting virions. in both the ie175(icp4) and ie110(icp0) promoters, this activation requires an upstream cis-acting target response element that contains a single taatgarat consensus element. furthermore, many hsv ie taatgarat elements overlap with atgctaat octamer motifs that are similar to the ... | 1989 | 2542590 |
| acute tonsillitis in young men: etiological agents and their differentiation. | group a beta-hemolytic streptococci were found in 38% of 257 young men in military service with acute tonsillitis. of the 108 patients tested also for viral antibodies, 42% showed a 4-fold rise in antibody titers. adenovirus was the most frequent (31%) nonstreptococcal agent, followed by epstein-barr virus (6%), and influenza virus (5%). non-group a streptococci were isolated in about the same proportion (18%) as in healthy control subjects. other data also suggested that these bacteria were car ... | 1989 | 2543062 |
| clinical and microbiologic findings in lambs inoculated with pasteurella haemolytica after infection with ovine adenovirus type 6. | colostrum-deprived lambs (10 to 20 days old) were inoculated with either ovine adenovirus type 6 (oav-6; n = 6), pasteurella haemolytica type a1 (n = 6), or oav-6 followed by p haemolytica 5 days later (n = 10). another group (n = 3) served as sham-inoculated controls. lambs inoculated with oav-6 or p haemolytica developed mild and moderate respiratory tract disease of 6 and 3 days' duration, respectively. lambs inoculated with virus and bacteria developed clinical signs of respiratory tract dis ... | 1989 | 2543241 |
| in situ hybridization studies of adenoviral infections of the lung and their relationship to follicular bronchiectasis. | we have used a probe against the adenovirus genome to study cultured epithelial cells specifically infected with various types of adenovirus and graham 293 cells, which contain few copy numbers of a fraction of the adenovirus genome. we have also examined lung tissue obtained from three cases of acute adenovirus pneumonia, two cases of adenovirus pneumonia that had passed through the acute phase, and nine cases of follicular bronchiectasis. our purpose was to determine whether the probe was effe ... | 1989 | 2543250 |
| dna-binding activity of the adenovirus-induced e4f transcription factor is regulated by phosphorylation. | previous experiments have identified e4f, an inducible cellular factor that binds to sequences in the adenovirus e4 promoter that are critical for e1a-dependent transcriptional activation. the e4f factor has been purified and shown to stimulate transcription in vitro from the e4 promoter. analysis of the affinity-purified factor identifies a single polypeptide of 50 kd that has e4f-specific binding activity. e4f binding activity is also regulated during f9 cell differentiation and can be activat ... | 1989 | 2545525 |
| herpes simplex virus type 1 immediate-early protein vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system. | reactivation of latent herpes simplex virus type 2 (hsv-2) by the immediate-early protein vmw110 was studied by using an in vitro latency system. adenovirus recombinants that express vmw110 reactivated latent hsv-2. an hsv-1 mutant possessing a deletion in a carboxy-terminal region of vmw110 reactivated latent hsv-2, whereas mutant fxe, which has a deletion in the second exon, did not. therefore, vmw110 alone is required to reactivate latent hsv-2 in vitro, and the region of vmw110 defined by th ... | 1989 | 2545921 |
| evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens. | a commercial monoclonal antibody-based enzyme immunoassay (adenoscreen; mercia diagnostics ltd., guildford, united kingdom) for the detection of adenovirus types 40 and 41 in stool specimens was evaluated. two assay modes were tested. in the first, 177 stool samples were screened for the presence of adenovirus type 40 or 41 (assay mode 1). virus was detected in 79 of 82 specimens positive for adenovirus type 40 or 41 by a polyclonal antibody-based immune electron microscope test, giving a sensit ... | 1989 | 2546968 |
| comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections. | a comparison of immunofluorescence (if), enzyme-linked immunosorbent assay (elisa), and isolation in tissue culture (tc) for detection of respiratory viruses was performed on 496 nasopharyngeal aspirates from children under 5 years of age with lower acute respiratory infections who were receiving attention at three hospitals in buenos aires, argentina. all samples were tested by the three methods for respiratory syncytial virus (rsv), influenza a and b, adenovirus, and parainfluenza 1 and 3. vir ... | 1989 | 2547019 |
| infection by enteric adenoviruses, rotaviruses, and other agents in a rural african environment. | from february 1985 to january 1986, 432 stool samples, 310 from rural african children with diarrhea and 122 from controls, were analysed for the presence of enteric viruses known to be associated with diarrhea. group a rotavirus elisa indicated 12.9% positivity among patients and 2.5% positivity among controls. only 23 of the 43 rotavirus elisa-positive stools were also positive by electron microscopy. nine children, three of whom were controls, were found to be shedding coronavirus-like partic ... | 1989 | 2547021 |
| in situ hybridization: an optimised detection protocol for a biotinylated dna probe renders it more sensitive than a comparable 35s-labelled probe. | the development of a highly sensitive biotinylated in situ hybridization protocol using egg drop syndrome virus infection of domestic fowl as a model is described. this protocol incorporates the use of a monoclonal antibody to biotin as the initial step in a peroxidase-based detection system. the detection of viral nucleic acid in formalin-fixed, paraffin-embedded sections using biotinylated and 35s-labelled probes is compared to the detection of viral antigen by immunocytochemistry. biotinylate ... | 1989 | 2547814 |
| double-label immunofluorescence method for simultaneous detection of adenovirus and herpes simplex virus from the eye. | the development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. in this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 x g for 1 h, incubated at 37 degrees c for 48 h, and fixed in methanol at room temperature for 15 min. the virus inclusions were detected by staining with a double-label indirect immunofluorescence ... | 1989 | 2549090 |
| [detection of viral nucleic acid sequences in paraffin and cryostat sections of clinical study samples using in situ hybridization]. | | 1989 | 2549569 |
| animal virus dna replication. | | 1989 | 2549858 |
| the serological response to foot-and-mouth disease vaccination is not affected by simultaneous infectious bovine rhinotracheitis/adenovirus/parainfluenza 3 vaccination. | young calves were simultaneously vaccinated by subcutaneous route against foot-and-mouth disease (fmd) and against infectious bovine rhinotracheitis/adenovirus/parainfluenza-3 (ibr/adeno/pi-3) by intranasal route. the serological response against the 3 fmd virus types of the fmd vaccine was clearly positive. there was no significant difference between results of simultaneous fmd and ibr/adeno/pi-3 vaccination and fmd vaccination only. | 1989 | 2549906 |
| observations of experimentally induced quail bronchitis. | quail bronchitis was experimentally reproduced in captive-reared bobwhite quails (colinus virginianus). quails were inoculated with 10(6) mean tissue culture infective doses of quail bronchitis virus at 1,3,6, or 9 weeks of age by the intratracheal, intraperitoneal, or subcutaneous route. clinical signs were minimal, but occasionally birds were ruffled, exhibited open-mouthed breathing, and developed "snicks." mortality rates of quails inoculated at 1 or 3 weeks ranged from 7% to 87%. quails ino ... | 1989 | 2549936 |
| frequency of cell transformation by the small dna tumor viruses: infection of proliferating cells and quiescent cells. | small dna-containing tumor viruses (simian virus 40, mouse polyoma-virus, and adenoviruses) malignantly transform fibroblasts of the susceptible rodents. fibroblasts can exist, in vitro and in vivo, in either of the two states: the proliferating state or the quiescent state. in the present study, we examined whether the state of fibroblasts at the time of exposure to these dna viruses affects the frequency of transformation. dense-focus formation in monolayer culture of rat 3y1 fibroblasts was u ... | 1989 | 2550745 |
| stereospecific relationships between elements in an sv40/adenovirus-2 heterologous promoter. | spacing mutations have been constructed between an sv40 72 bp enhancer element and the adenovirus-2 major late promoter upstream element (ad2mlp-ue), the ad2mlp-ue and the ad2mlp tata-box and the sv40 72 bp enhancer and the tata-box in the absence of the ad2mlp-ue. a clear periodic pattern of transcription from the ad2mlp was obtained by inserting multiples of half of a dna turn between the sv40 enhancer and the ad2mlp-ue. we interpret this as a stereospecific requirement for the spatial alignme ... | 1989 | 2550902 |
| hemorrhagic cystitis associated with urinary excretion of adenovirus type 11 following allogeneic bone marrow transplantation. | we studied a total of 50 recipients who had received allogeneic bone marrow transplantation (bmt) and evaluated both the presence of hemorrhagic cystitis (hc) and the urinary excretion of adenovirus. twelve recipients developed hc and eight of these 12 patients excreted adenovirus type 11 at the onset of cystitis. urine for virus isolation was attempted 30, 60 and 100 days after bmt. among 137 specimens examined, eight were positive for adenovirus type 11. of these eight samples, six were collec ... | 1989 | 2551434 |
| [epidemic keratoconjunctivitis. laboratory study of patients infected with adenovirus type 8]. | the authors tried to detect, using different methods, the causal agent of the disease in eight patients with epidemic keratoconjunctivitis. from the conjunctival sacs of one ambulatory patient and six of seven patients hospitalized on account of other eye diseases they isolated 11 strains of adenovirus type 8 on cell cultures from human embryonic lungs. using direct and indirect elisa, they detected adenovirus antigen in four of seven examined smears from the conjunctival sac without previous cu ... | 1989 | 2551515 |
| [nosospecific features of the dynamics of immunological parameters in young children with acute respiratory virus infections of varying etiology]. | the study of acute viral respiratory infections has shown that in the course of these infections fluctuations in the content of antigen-nonspecific immunocytes and immunoglobulins occur, depending on the etiology of the disease. this dependence should be taken into consideration in the evaluation of the immune status of young children with acute viral respiratory infections. | 1989 | 2552713 |
| differentiation of avian adenovirus type-ii strains by restriction endonuclease fingerprinting. | three serologically indistinguishable viruses from the avian adenovirus type-ii splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. the dna from these viruses were examined with 6 restriction endonucleases (bgl ii, ecori, hindiii, hha i, xho i, and bamhi). markedly different dna cleavage patterns were found in these virus isolates with all the 5 enzymes, except with bamhi, ... | 1989 | 2552877 |
| adenovirus enteritis in siv-infected rhesus monkeys. | | 1989 | 2553828 |
| a review of marble spleen disease of ring-necked pheasants. | marble spleen disease (msd) is a contagious disease of captive-reared ring-necked pheasants (phasianus colchicus) characterized by variable mortality and high morbidity. the etiologic agent is a type ii avian adenovirus closely related to hemorrhagic enteritis virus of turkeys and splenomegaly virus of chickens. gross lesions of msd consist of enlargement and mottling of the spleen and pulmonary edema. microscopic alterations are most pronounced in the spleen and are characterized by reticuloend ... | 1989 | 2553999 |
| serologic survey for infectious canine hepatitis virus in grizzly bears (ursus arctos) from alaska, 1973 to 1987. | serum antibody prevalence of infectious canine hepatitis virus was 12% (90 of 725) for grizzly bears (ursus arctos) from alaska (usa) during the period 1973 to 1987. prevalence was highest on kodiak island at 29% (37 of 127). prevalence of exposure at individual collection areas did not change significantly over time. there were no significant sex-specific differences in prevalence. prevalence was directly related to age, but it was 0% for bears less than 2-yr-old. young bears which are exposed ... | 1989 | 2554000 |
| [opportunistic viral infections in aids]. | authors pointed on etiologic connections between particular clinical syndroms in aids and microbial, parasitic and viral agents belonging to different taxonomic groups. participation of viruses in etiologic, immunology, pathology and importance for chemotherapy pointing on proved and probable relations, were analysed. valuable observations related with etiologic and opportunistic viral mechanisms of coinfections were mentioned, as well as main diagnostic aspects. references limited up to the mos ... | 1989 | 2554368 |
| [respiratory viral infections in young children 1985-1988]. | in the period from may 1985 to june 1988 the authors (using the immunofluorescence method) examined 848 children aged 0-2 years hospitalized due to infections of respiratory tracts in the ii clinic of pediatrics of the pediatrics department of the ii medical faculty in the medical academy in warsaw. the most frequent causes of infections in respiratory tracts were virus rs (21.1% of patients) and virus of parainfluenza type 3 (6.5% of patients). an increase in viral respiratory infections took p ... | 1989 | 2554369 |
| an improved nuclear extract preparation method. | a rapid, efficient, and highly reproducible procedure for nuclear extract preparation is described. the method uses lysolecithin (lysophosphatidylcholine) to disrupt plasma membranes and requires no detergents or douncing. soluble extracts prepared by this method are comparable to conventional nuclear extracts in all assays tested. lysolecithin nuclear extracts are competent for rna polymerase ii and iii transcription, dna replication, pre-mrna splicing, and sequence specific dna-protein binding ... | 1989 | 2555292 |
| rapid culture confirmation of adenovirus isolates using the adenoclone enzyme immunoassay (eia) test kit. | the adenoclone enzyme immunoassay (eia) test kit (cambridge bioscience corp., worcester, ma) was evaluated using 43 adenovirus and 53 nonadenovirus (i.e., polio-, echo-, coxsackievirus types a,b, parainfluenza virus, and herpes simplex virus) isolates obtained during routine clinical diagnostic screening procedures. forty-two of 43 confirmed adenovirus isolates were reactive upon testing by the adenoclone test kit. none of the 53 nonadenovirus isolates, including seven difficult-to-cultivate gro ... | 1989 | 2556237 |
| recovery of viruses and bacteria in waters off bondi beach: a pilot study. | a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ... | 1989 | 2556634 |
| initiation of eukaryotic dna replication in vitro. | | 1989 | 2557059 |
| is a persistent adenovirus infection involved in coeliac disease? | recent evidence has implicated adenovirus 12 in the aetiology of coeliac disease so that persistent infection by this virus must be considered. we have undertaken a search for adenovirus dna in duodenal biopsy samples from a total of 26 coeliac and non-coeliac patients. we could find no evidence of persistent virus dna by southern blot techniques even under conditions which approach a sensitivity of one copy of virus genome per cell, and use either adenovirus 12 or 41 dna. | 1989 | 2557269 |
| four factors are required for 3'-end cleavage of pre-mrnas. | we reported previously that authentic polyadenylation of pre-mrnas in vitro requires at least two factors: a cleavage/specificity factor (csf) and a fraction containing nonspecific poly(a) polymerase activity. to study the molecular mechanisms underlying 3' cleavage of pre-mrnas, we fractionated csf further and show that it consists of four separable subunits. one of these, called specificity factor (sf; mr, approximately 290,000), is required for both specific cleavage and for specific polyaden ... | 1989 | 2558045 |
| a new subgroup 2 bovine adenovirus proposed as the prototype strain 10. | a slowly growing subgroup 2 bovine adenovirus (bav) strain designated ruakura 78-5371 was isolated from a yearling heifer with systemic adenovirus infection. cross neutralization tests and restriction endonuclease analysis of the viral dna showed the virus to be distinct from the other 9 recognised types of bav. it is proposed that this strain should be regarded as the prototype strain of the new type bav-10. | 1989 | 2558628 |
| virus infection induces redistribution and membrane localization of the nuclear antigen la (ss-b): a possible mechanism for autoimmunity. | to investigate the possibility that anti-la (ss-b) antibodies in sjögren's syndrome were induced by virus infection we studied the distribution of la in virus-infected human cell lines. three monoclonal antibodies to la were used with monoclonal anti-sm (derived from mrl/lpr lupus mice) and anti-rat immunoglobulin antibodies as controls. in uninfected cells la was predominantly in the nucleus. twenty-four hours after infection of hep-2 cells with adenovirus 2, the la and sm antigens appeared to ... | 1989 | 2558824 |
| concurrent adenoviral and herpetic ocular infections. | we report two cases of a previously unrecognized clinical entity: concurrent adenoviral and herpetic ocular infection. the first case was recognized clinically by the presence of herpetic keratitis and findings attributable to epidemic keratoconjunctivitis (ekc) including lid swelling, pseudomembranous and follicular conjunctivitis, subconjunctival hemorrhages, preauricular lymphadenopathy, and corneal infiltrates. only herpes simplex virus was recovered by culture. in the second case of apparen ... | 1989 | 2559639 |
| viral contamination of some drinking waters in romania: a twenty years survey. | a synthesis is made on the 20 years virological survey of the drinking water from some towns of romania. the sampling of the water was made by the gauze-pad method. the virus concentration method by adsorption-elution with the yeast cells was applied concomitantly, at first with the pe60 method, then, during the last years, either with aluminum hydroxide or with the polymer pv methods, and the concentrates were inoculated both into suckling mice and into cell cultures. various types of coxsackie ... | 1989 | 2560479 |
| [research on antibodies against bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, rotavirus and coronavirus in cattle in western zaire: complementary results]. | two-hundred bovine sera from western zaire were screened for antibodies to 8 viruses: bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, bovine rotavirus and bovine coronavirus. positive sera were found to all these viruses. for animals whose origin was undoubted, the main features were the high prevalence of infections by rotavirus and bhv-4 and the low prevalence of infections by coronavirus and bvd-md virus. | 1989 | 2560579 |
| current use and future directions of adenovirus vaccine. | adenoviruses are a major cause of respiratory illnesses in military recruits and also are common causes of respiratory and gastrointestinal infections during childhood. forty-one serotypes of human respiratory and enteric adenoviruses have been identified. live, oral adenovirus vaccines developed for the military and tested in large clinical trials have proved to be safe and highly effective in decreasing hospitalizations related to adenoviral acute respiratory diseases. studies have demonstrate ... | 1989 | 2560582 |