| equine herpesviruses. experimental infection of a foetus with type 2. | | 1977 | 201235 |
| isolation of equine rhinovirus type 1. | | 1977 | 201238 |
| [occurrence and histogenesis of various fetal pneumopathies in equine viral abortion]. | | 1977 | 201242 |
| definition of cytochrome c binding domains by chemical modification. ii. identification and properties of singly substituted carboxydinitrophenyl cytochromes c at lysines 8, 13, 22, 27, 39, 60, 72, 87, and 99. | sensitive thin layer peptide mapping is employed to establish the identity and the homogeneity of eight singly substituted 4-carboxy-2,6-dinitrophenyl derivatives of horse cytochrome c. seven of the components, all of greater than 95% homogeneity, are modified at lysyl residues 13, 72, 87, 8, 27, 39, and 60. the eighth component is a mixture of derivatives at lysines 22 and 99. the positions of the modified residues were confirmed by the amino acid analysis and edman sequential degradation of th ... | 1978 | 201615 |
| genetic relatedness of equine herpesvirus types 1 and 3. | the genetic relatedness of two types of equine herpesviruses (ehvs), 1 (ehv-1) and 3 (ehv-3), was determined by dna-dna reassociation kinetics. denatured, labeled viral dna probe was allowed to reassociate in the presence or absence of the second unlabeled viral dna. the initial rate of reassociation of either labeled viral dna was increased by the presence of the heterologous viral dna to an extent indicating only 2 to 5% homology between the two ehv genomes. moreover, labeled rna extracted fro ... | 1977 | 201775 |
| the role of oestrogen replacement in the climacteric syndrome. | using a double-blind placebo-controlled cross-over design, the effect of equine conjugated oestrogens tablets (premarin) was studied in 20 women with the climacteric syndrome followed during 15 months. sixteen women were equally improved on placebo and oestrogen. only 2 patients had an improved sense of well-being on oestrogen and not on placebo. the psychological diagnosis was unrelated to the subjective response to oestrogen or placebo. performance in psychological tests administered before an ... | 1977 | 201958 |
| viruses associated with renal disease of man and animals. | | 1977 | 201965 |
| complement-fixation reactions in equine viral arteritis. | | 1977 | 202179 |
| demonstration of equine infectious anemia virus in primary leukocyte cultures by electron microscopy. | electron microscopy was used to demonstrate the presence of viral particles in primary cultures of leukocytes taken from a horse after sc inoculation with the wyoming strain of equine infectious anemia virus. unlike previous studies, the exposure virus was not passaged through cell culture prior to horse inoculation. cultures were begun approximately 1 week before and 1 week after the 1st pyrexic period after inoculation. in both samples, viral particles and cytoplasmic alterations were observed ... | 1977 | 202180 |
| electron microscopic studies on equine infectious anemia virus (eiav). brief report. | morphological studies of eiav reveal knobs on the surface of the particles, conically and tubularly shaped cores, budding particles with dense crescents directly underlying the plasma membrane, and distinct intracytoplasmic structures in infected cells. | 1977 | 202230 |
| nuclear magnetic resonance studies of hemoproteins. viii. proton nmr studies of the binding of various nitrogenous ligands to ferric cytochrome c. | the structure of the heme environment of horse heart ferric cytochrome c was examined in the presence of various nitrogenous bases at several temperatures with the aid of hyperfine shifted proton nmr spectra at 220 mhz. the resonance positions and line widths of the signals for the peripheral methyl groups of the heme exhibited distinctive features of its low-spin state characteristic of each external ligand. in the imidazole complex of ferric cytochrome c, remarkable line sharpening of the heme ... | 1978 | 202330 |
| a fluorescence study of the binding of cytochrome c to mixed-phospholipid microvesicles : evidence for a preferred orientation of the bound protein. | kinetic and equilibrium experiments are reported on the binding of the fluorescent probe 1,8-anilino-naphtalene sulfonate (ans) to microvesicles of natural lecithin containing 10 per cent of an anionic phospholipip (90 : 10 mixtures). kinetics discriminated between fast binding to the outer leaflet of the bilayer and apparently slow binding to the inner leaflet controlled by the diffusion of the probe across the bilayer. the equilibrium distribution of ans between the two leaflets was not depend ... | 1977 | 202339 |
| [long-term studies on the causes of abortion in the breeding of pure breeds]. | | 1977 | 202446 |
| changes in enzyme activities during the artificially stimulated transition from follicular to luteal cell types in rat ovary. | this paper reports the time course of development of the intramitochondrial cholesterol side-chain-cleavage activity, cytoplasmic nadp+-dependent isocitric dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase during ovarian maturation, using as a model the immature rat ovary stimulated to develop with pharmacological doses of gonadotrophin. these enzymic activities were correlated with increases in ovarian content of dna, cellular content of adenosin 3':5'-monophosphate, and the lev ... | 1977 | 202454 |
| the crystal structure of complexes between horse liver alcohol dehydrogenase and the coenzyme analogues 3-iodopyridine-adenine dinucleotide and pyridine-adenine dinucleotide. | we have studied the binding of the enzymatically active nad+ analogue, 3-iodopyridine-adenine dinucleotide, and the inactive analogue, pyridine-adenine dinucleotide to the enzyme horse liver alcohol dehydrogenase using x-ray crystallographic methods. these studies were made under such conditions that crystals of the complexes were isomorphous to apoenzyme crystals. both analogues bind in the same conformation. the binding of the adenosine moiety is very similar to that of adp-ribose or nadh boun ... | 1977 | 202459 |
| the structural polypeptides of equine infections anemia virus. | the structural polypeptides and glycoproteins of equine infectious anemia virus were identified following electrophoresis in sds-page. the major non-glycosylated polypeptides had molecular weights of 25,000, 14,000 and 11,000 daltons. two glycoproteins of 80,000 and 40,000 daltons were also detected. the relationship of these components to the structural elements of mammalian c-type oncoviruses is discussed. | 1978 | 202575 |
| characterization of a fetal calf serum-derived molecule reactive with human natural antibodies: its occurrence in tissue culture-grown type c rna viruses. | by means of a sensitive radioimmunoprecipitation (rip) assay, simian sarcoma virus-simian sarcoma-associated virus (ssv-ssav), purified from culture fluids of infected normal rat kidney (nrk) cells, was shown to acquire a surface antigen from serum used in the tissue culture medium. this antigen, which was acquired when serum from either fetal calf, horse, swine, rabbit, or chicken origin was used, accounted for a substantial portion (but not all) of the total precipitating activity exhibited by ... | 1978 | 202656 |
| preovulatory changes in ovarian cyclic amp and prostaglandins in immature rats injected with pmsg. | in 28-day-old rats injected with pmsg, ovarian cyclic amp and pge and pgf increased 2 and 3-4 h respectively after the lh peak. | 1978 | 202707 |
| gonadotropin receptors, camp and testosterone in estrogenized male rats. | | 1978 | 203479 |
| effect of human chorionic gonadotrophin and ovine luteinizing hormone on rat ovarian macromolecular metabolism. | administration of human chorionic gonadotrophin (hcg) or ovine lh to immature rats primed with pregnant mare serum gonadotrophin (pmsg) stimulated the rate of synthesis of polyadenylic acid (poly a)-rich rna in the ovaries. the rate of total rna synthesis was not affected significantly by hormone treatment, whereas protein synthesis was enhanced. the increase in the rate of synthesis of poly(a)-rich rna in the ovaries could be inferred as induction of messenger rna synthesis after the hormone tr ... | 1978 | 203645 |
| serological studies of mucosal disease virus in england and wales. | a survey of the cattle population in england and wales showed that about 62 per cent of animals had neutralising (sn) antibody to bvd-md virus in their serum. fewer seropositive individuals were detected by immunodiffusion (53 per cent), and complement fixation (cf) tests (34 per cent). the prevalence of antibody was not affected by herd size, but some variation was found between various regions of the country, and the proportion of animals seropositive rose markedly with age. the immunodiffusio ... | 1978 | 203990 |
| the sequence of hormonal changes during prostaglandin induced luteolysis of the superluteinized rat ovary. | | 1978 | 204145 |
| serological evidence of infection of tacaribe virus and arboviruses in trinidadian bats. | sera from 39 species of bats collected in trinidad between 1972 and 1974 were tested against some or all of 18 arboviruses in hemagglutination inhibition (hi) and/or suckling mouse neutralization (n) tests. a few sera were hi-positive with mucambo, eastern equine encephalitis (eee), oriboca, restan, manzanilla, guama, bimiti, and catu. no sera were hi-positive with mayaro, caraparu or maguari. many ser inhibited one or more of the group b hemagglutinins: ilheus, st. louis encephalitis (sle), den ... | 1978 | 204207 |
| diagnosis of equine neurologic problems. | this is a review of the more common diseases of the spinal cord and various areas of the brain of horses. the results of a two and one-half year study of spinal cord disease are emphasized. after a description of the lesion the salient clinical signs are described and the features that differentiate them from other similar diseases. in the seminar, films of case and slides of lesions will be shown to document these diseases. | 1978 | 204449 |
| clinicopathological manifestations of selected neoplasms. | this report provides a general overview of the pathobiology of neoplasia, and an update on the clinicopathological manifestations of lymphosarcoma, mastocytoma, histiocytoma, melanoma, sarcoid and circumanal gland tumors in domestic animals. neoplasia represents a continuum of events from reversible hyperplasia to irreversible and pathological changes in tissue growth patterns. in some instances the causes of this disease process have been identified, but the etiology of the majority of naturall ... | 1978 | 204450 |
| circular dichroic properties and conformation of thionicotinamide dinucleotides bound to horse-liver alcohol dehydrogenase. | the interaction between horse liver alcohol dehydrogenase and the oxidized and reduced forms of the 3-thionicotinamide--adenine dinucleotide coenzyme analogues (snad and snadh) has been investigated by ultraviolet absorption, fluorescence and circular dichroism. the fluorescence of snadh is enhanced when bound to the enzyme, and the protein fluorescence is quenched by both snadh (60--65%) and snad (65%). the possible origin of the larger quenching produced by snad with respect to that of nad is ... | 1978 | 204483 |
| fluorescence properties of reduced thionicotinamide--adenine dinucleotide and of its complex with octopine dehydrogenase. | reduced 3-thionicotinamide--adenine dinucleotide (snadh) is shown to be fluorescent, with an emission maximum at 510 nm when excited in the region of the absorption maximum (398 nm), and with a very low quantum yield, (3.4 +/- 0.5) x 10(-4). the interaction between snadh and octopine dehydrogenase was investigated by ultraviolet-difference spectroscopy and fluorescence. some surprising fluorescence features were found when snadh was bound to the enzyme in the presence of d-octopine, as follows. ... | 1978 | 204484 |
| studies on an energy structure-function relationship of dehydrogenases. i. calorimetric investigations on the interaction of coenzyme fragments with horse liver alcohol dehydrogenase. | | 1978 | 204522 |
| the interaction of cytochrome c with phospholipids. a pulse-radiolysis study. | the reactions of the hydrated electron (eaq-), produced during pulse radiolysis, have been used to study the binding of phosphatidyl choline (pc), phosphatidyl serine (ps), phosphatidyl ethanolamine (pe), and phosphatidyl inositol (pi) vesicles with horse-heart cytochrome c. an interaction could only be detected between cytochrome c and either ps or pi. an apparent equivalence point in the binding was reached for both phospholipids at a molar ratio of phospholipid : protein of 6 : 1. at this poi ... | 1978 | 204592 |
| pregnant mare serum gonadotrophin and follicle-stimulating hormone stimulation of cyclic amp production in rat seminiferous tubule cells. | pregnant mare serum gonadotrophin (pmsg) was found to stimulate the production of cyclic amp in isolated rat seminiferous tubule cells. the pmsg was equipotent with highly purified ovine fsh. the subunits of pmsg were inactive in this system but recombination resulted in partial restoration of activity, and the combination of pmsg alpha-subunit with fsh beta-subunit was even more active. desialation of pmsg, but not of ovine fsh, resulted in a reduction of slope and potency of this response, sug ... | 1978 | 204723 |
| equine rhinopneumonitis vaccine: immunogenicity and safety in adult horses, including pregnant mares. | | 1978 | 205144 |
| induction of a cell membrane antigen by equine infectious anemia virus. | equine fibroblasts persistently infected with equine infectious anemia virus acquire a new cell membrane antigen demonstrable by indirect radioimmunoassay, using infected horse serum as an antibody source. | 1978 | 205145 |
| [demonstration of a clinically manifested mixed reovirus serotype iii and rhinopneumonitis virus infection in a horse]. | | 1978 | 205205 |
| assignment of the heme c resonances in the 360 mhz h nmr spectra of cytochrome c. | in the 360 mhz 1h nmr spectra of horse heart ferrocytochrome c recorded after suitable digital resolution enhancement, the resonances of all the heme c protons with the exception of those of the propionic acid side chains were observed as well resolved lines. from spin decoupling and nuclear overhauser effects in homonuclear double resonance experiments, all these resonances were assigned to their respective positions in heme c. with saturation transfer experiments in solutions of partially redu ... | 1978 | 205265 |
| heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera. | bovine sera for tissue culture use were shown to contain heterophil antigen of the paul-bunnell-davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. the antibody titers observed were comparable to those determined by two other methods; i.e., the immune adherence hemagglutination test was found to be as specific as the differential absorption test with horse erythrocytes or the ox cell hemolysis assay,but it appeared to be the most sensi ... | 1978 | 205510 |
| the effect of nalidixic acid group compounds on reduction of cytochrome c from horse heart and candida krusei. | reduction of cytochrome c from both horse heart and candida krusei by feso4 has been demonstrated. this reaction was stimulated by nalidixic acid and structurally related compounds, and the effect was more pronounced for the yeast cytochrome. divalent metal ions other than fe2+ lessened or abolished the stimulation by these compounds. fe2+ and other metal ions altered the spectra of nalidixic acid and related compounds indicating the formation of metal chelate complexes. 1,10-phenanthroline inhi ... | 1978 | 205625 |
| [occurrence and routine detection of clostridium perfringens in the horse]. | | 1978 | 206257 |
| species of differences in postganglionic motor transmission to the retractor penis muscle. | 1 graded motor responses were elicited in isolated, desheathed, thin strips of dog, horse, pig and sheep retractor penis (rp) muscles by field stimulation with trains of 0.2 ms pulses at 10 hertz. these twitches were shown to be neurogenic in all four species, by their prompt extinction in tetrodotoxin.2 alpha-adrenoceptor blocking drugs abolished the contractile response to noradrenaline and to tyramine in all four species.3 motor transmission was wholly adrenergic in the horse as in the dog rp ... | 1978 | 206306 |
| equilibrium and kinetic studies of unfolding of homologous cytochromes c. | extensive investigations of the unfolding equilibria and kinetics of oxidized and reduced cytochromes c are reported. it is found that all cytochromes c have similar unfolding free energies (deltagd = 7 +/- 1 kcal/mol). differences among species do not correlate in any way with the metabolic differences among species. the stabilization of cytochrome c on reduction is estimated at 1.1 kcal/mol. stability differences among species are mirrored in their denaturation kinetics. for cytochrome c (iii) ... | 1978 | 206558 |
| preparation of hemagglutinating antigen of equine infectious anemia virus from infected equine leukocyte cultures. | | 1978 | 206841 |
| absence of adenosine deaminase activity in a mammalian cell line transformed by rous sarcoma virus. | no detectable adenosine deaminase activity was found in whole cells or 105,000g cytosol preparations of b-mix k-44/6 cells when either [3h]adenosine or [3h]arabinosyladenine was used as substrate. when grown in tissue culture medium supplemented with horse serum these cells provide a deaminase-free system not requiring the use of an adenosine deaminase inhibitor. | 1978 | 206965 |
| isolation of equine herpesvirus type 2 from foals, showing respiratory symptoms. | | 1978 | 207057 |
| isolation and characterisation of an equine rhinovirus. | | 1978 | 207059 |
| perinatal foal mortality associated with a herpesvirus. | | 1977 | 207253 |
| generalised congenital equine herpes virus infection in a neonatal foal. | | 1977 | 207256 |
| circular dichroism and circular polarization of luminescence of reduced nicotinamide adenine dinucleotide in solution and bound to dehydrogenases. | the cd (circular dichroism) and cpl (circular polarization of luminescence) spectra of nadph in aqueous solution were studied and found to be markedly different. the spectra were not affected by cleavage of the coenzyme molecule with phosphodiesterase. the differences are thus not due to the existence of extended and folded conformations of nadph and it is concluded that they originate in excited state conformational changes of the nicotinamide--ribose fragment. opposite signs of both the cd and ... | 1978 | 207311 |
| effects of culture conditions on the growth and differentiation of transformed rat adrenocortical cells. | normal rat adrenocortical cells grow in vitro in two states of differentiation, depending on culture conditions. under one set of conditions, they proliferate and are fibroblast shaped, with some myoid cell characteristics and with limited steroidogenic activity. under alternate conditions they do not replicate and are epithelial-like and similar to adrenocortical secretory cells structurally and steroidogenically. the effects of oncogenic transformation on these cellular responses to culture va ... | 1978 | 207413 |
| study of follitropin receptors in testis using a homologous system. binding of porcine follitropin to plasma membranes from immature porcine testis and correlation with adenylate cyclase stimulation. | the properties of follitropin receptors in immature porcine testis were determined using highly purified porcine follitropin. 1. the characteristics of follitropin binding to a subcellular fraction rich in plasma membranes were studied using a 125i-labelled follitropin with high specific activity (75-100 ci/g) and high binding activity. the binding is dependent on time, temperature and ph. it is specific to follitropin as demonstrated by the very low binding activity of the follitropin alpha and ... | 1978 | 207514 |
| covalent binding of an nad analogue to liver alcohol dehydrogenase resulting in an enzyme-coenzyme complex not requiring exogenous coenzyme for activity. | 1. the nad analogue, n6-[n-(6-aminohexyl)carbamoylmethyl]-nad, was covalently bound to horse liver alcohol dehydrogenase in a carbodiimide-mediated reaction and in such a way that it was active with the very same enzyme molecule to which it was coupled. 2. the degree of substitution, i.e. the number of nad analogues per enzyme subunit, could be varied (0.3-1.6). in one preparation 1.6 coenzyme molecules were bound per subunit; the alcohol dehydrogenase activity of this preparation was 40% of the ... | 1978 | 207526 |
| pregnant mare serum gonadotropin exhibits higher affinity for lutropin than for follitropin receptors of porcine testis. | | 1978 | 207581 |
| measurement of neutralizing antibody to equid herpesvirus 1 by single radial hemolysis. | antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. | 1978 | 207720 |
| steady-state study of horse-liver adh: detection of two kinetically heterogeneous components. | new theoretical considerations and a new approximation strategy were applied to the kinetic analysis of the experimental relationship between the reaction velocity in the steady state and the concentrations of ethanol and nad. it could be shown that horse-liver adh consists of two kinetically heterogeneous components. | 1978 | 207974 |
| [cytomorphological aspects of the blood experimentally induced with inoculation of equine infectious anemia virus]. | | 1978 | 208135 |
| maintenance of foals with combined immunodeficiency: causes and control of secondary infections. | sixty-six cases of combined immunodeficiency (cid) in foals were studied to determine the most prevalent causes of infection and death. lesions of the respiratory system were observed in 59 of the foals and were attributable to infection with equine adenovirus. pneumocystis carinii, and bacteria. significant lesions were also observed in liver, pancreas, intestines, heart, and kidneys. maintenance of foals with cid for experimental purposes is directed at the prevention and control of these seco ... | 1978 | 208432 |
| equine grass sickness: serologic evidence of association with clostridium perfringens type a enterotoxin. | clostridium perfringens type a enterotoxin seroneutralization was carried out on sera from 50 horses recovered from grass sickness and from 100 other horses with no record of having had the disease. of the affected horses, 70% had seroneutralizating titers higher than 1:64, half of these being equal or higher than 1:128. more than 88% of the horses with no record of grass sickness had titers lower than 1:64. these data support the theory of association between c perfringens type a toxins and gra ... | 1978 | 208433 |
| inhibition by ethanol, acetaldehyde and trifluoroethanol of reactions catalysed by yeast and horse liver alcohol dehydrogenases. | 1. produced inhibition by ethanol of the acetaldehyde-nadh reaction, catalysed by the alcohol dehydrogenases from yeast and horse liver, was studied at 25 degrees c and ph 6-9. 2. the results with yeast alcohol dehydrogenase are generally consistent with the preferred-pathway mechanism proposed previously [dickenson & dickinson (1975) biochem. j. 147, 303-311]. the observed hyperbolic inhibition by ethanol of the maximum rate of acetaldehyde reduction confirms the existence of the alternative pa ... | 1978 | 208509 |
| estimation of rate and dissociation constants involving ternary complexes in reactions catalysed by yeast alcohol dehydrogenase. | stopped-flow studies of oxidation of butan-1-ol and propan-2-ol by nad(+) in the presence of phenol red and large concentrations of yeast alcohol dehydrogenase give no evidence for the participation of a group of pk(a) approx. 7.6 in alcohol binding. such a group has been implicated in ethanol binding to horse liver alcohol dehydrogenase [shore, gutfreund, brooks, santiago & santiago (1974) biochemistry13, 4185-4190]. the present result supports previous findings based on steady-state kinetic st ... | 1978 | 208510 |
| low-temperature studies of electron transfer between different cytochromes c and cytochrome c oxidase. | the ability of various native and modified cytochromes c to transfer electrons to cytochrome oxidase is compared in cytochrome c depleted beef heart mitochondrial particles. the kinetics are followed at -49 degrees c after the reaction is initiated by photolysis of the co compound of cytochrome oxidase in the presence of oxygen. horse, human, yeast iso-2, and carboxydinitrophenyl (cdnp)-lysine-60 horse cytochromes c all give initial rates of electron transfer that are equal to those observed in ... | 1978 | 208599 |
| follitropin receptors in rat testis. characterization with enzymatically 125i-labeled human follitropin. | the interaction between enzymatically radioiodinated human follitropin and the follitropin receptors in testis homogenate was investigated in immature and adult rats. the 125i-labeled human follitropin exhibited high binding activity with specific binding of up to 17% in the presence of an excess of testis homogenate. approx. 50% of the bound hormone could be eluted at ph 5, and the receptor purified tracer exhibited a 3.6-fold increase in binding activity when compared with the original tracer ... | 1978 | 208641 |
| the protective antigens of equine herpesvirus type 1. | equine herpesvirus type 1 was cultivated in swine testis cell cultures and partially purified by differential centrifugation and centrifugation in a linear sucrose density gradient. the viral envelope was separated from the nucleocapsid by treatment with rexol 25j followed by sucrose gradient centrifugation. the envelope and nucleocapsid preparations were then electrophoresed in polyacrylamide gel after solubilization with sodium dodecyl sulphate. hamsters were immunized with various preparation ... | 1978 | 208736 |
| viral infections in pregnancy. | the responsibility of the physician for viral infections in pregnancy has changed dramatically in the last 15 years. genital herpes infections have become much more frequent, and the recognition of these infections in women near term is particularly important. cytomegaloviruses have been found to be the most commom cause of congenital infection, but no satisfactory methods are available for prevention or treatment. varicella is now recognized as a teratogen when infection occurs in the first 4 m ... | 1978 | 208810 |
| detection of proviral dna in horse cells infected with equine infectious anemia virus. | equine infectious anemia virus (eiav) recently has been shown to possess a high-molecular-weight rna genome and a virion reverse transcriptase. we completed the demonstration that eiav is a retrovirus by showing the presence of proviral dna in equine cells infected in vitro, but not in normal horse dna. these studies were performed by using a highly representative cdna probe synthesized by the virion polymerase. it was found that this cdna reassociated extensively, and with high thermal stabilit ... | 1978 | 209211 |
| investigations on the presence of antibodies to alphaviruses, flaviviruses, bunyavirus and kemerovo virus in humans and some domestic animals. | the presence of antibodies to some alphaviruses, flaviviruses, bunyaviruses and orbiviruses was studied in the sera from humans and domestic animals of biotopes "c" and "t" in the south-east of romania. specific antibodies were found in humans to 3 alphaviruses (eastern equine encephalitis, western equine encephalitis and sindbis), in a very low proportion, and to 3 flaviviruses (tickborne encephalitis, west nile and ntaya), at a much higher percentage. very low percentages of antibodies to buny ... | 1978 | 209613 |
| [comparative analysis of the action of an interferon inducer in in vitro and in vivo systems]. | investigation of interferon inductor (poly ic) in vitro (chick embryon fibroblasts) and in vivo (mice) showed that the main parameters of the preparation effect, i. e. induction of interferon and development of resistance to viruses are as a whole quite comparable. the phenomenon of hyporeactivity in the both systems was reproduced on repeated use of the inductor. at the same time significant differences in the stimulating effect of deae-dextran were registered. the merits and demerits of the in ... | 1978 | 209734 |
| refolding and reactivation of liver alcohol dehydrogenase after dissociation and denaturation in 6m guanidine hydrochloride. | horse-liver alcohol dehydrogenase has been dissociated and denatured by 6 m guanidinium hydrochloride. removal of the denaturant under optimum conditions of the solvent leads to partial reactivation. the concentrations of the enzyme, as well as the coenzyme (nad+), and zn2+, affect the reactivation significantly, since high concentrations promote the formation of inactive aggregation products. analyzing the kinetics of reactivation and reassociation, conditions far from equilibrium of dissociati ... | 1978 | 210018 |
| the interaction of liver alcohol dehydrogenase with phenyl adenine dinucleotide, a novel analog of pyridine nucleotide coenzymes. | we have synthesized phenyl adenine dinucleotide (p1-adenosine-5')-p2-(beta-d-ribofuranosylbenzene-5')-pyrophosphate (phad), a novel analog of pyridine nucleotide coenzymes. this compound contains a planar aromatic ring, as does nad+, but lacks a positive charge. phad is an inhibitor of horse liver alcohol dehydrogenase, competitive with nadh. phad is very similar to nad+ sterically since both compounds have a planar aromatic ring. however, phad resembles nadh in binding to the enzyme because the ... | 1978 | 210161 |
| specific binding of human and bovine growth hormones to hypophysectomized rat hepatocytes. | specific binding of 125i-hgh and 125i-bgh was obtained in hypophysectomized rat liver cells. two types of somatogenic receptors were found with apparent dissociation constants of 4.2 x 10(8) m-1 and 1.7 x 10(7) m-1. the number of sites for the higher and lower affinity receptors were: 1 x 10(4) and 6 x 10(3), respectively. human, bovine and equine growth hormones appear to bind to liver cells with equal affinity, while ovine prolactin needs at least a two-order-magnitude greater concentration to ... | 1976 | 210628 |
| action of pmsg and asialo-pmsg on rat leydig and granulosa cells. | highly purified pregnant mares serum gonadotropin (pmsg) was nearly as potent as ovine luteinizing hormone (lh) and human follicle stimulating hormone (hfsh) when bioassayed in vitro in systems known to respond primarily to lh or fsh. an analogue of human chorionic gonadotropin treated with neuraminidase, galactosidase, beta-n-acetylglucosaminidase, and mannosidase (hcg) inhibited the stimulatory effects of hcg, lh, and pmgs on camp accumulation in rat leydig cells but did not inhibit the stimul ... | 1978 | 210675 |
| the occurrence of human wart-virus antibodies in dogs, pigs and cattle. | using the immunodiffusion method, antibodies against human wart-virus were detected in dog, pig and cattle sera but not in horse and reindeer sera. antibodies were found in 25 percent (28/114) of the dog sera, the prevalence of antibodies being fairly similar to that in children of the same age. by electron microscopy the antibodies in dog sera were shown to attach to and precipitate the human wart-virus particles, and in immunodiffusion the precipitation lines of human and dog sera were shown t ... | 1978 | 210743 |
| perinatal foal mortality associated with a herpesvirus. | an outbreak of perinatal foal mortality associated with a herpesvirus is described. twenty two foals either were still-born, or died soon after birth, or were weak and soon developed severe respiratory signs, or were normal at birth and developed respiratory symptoms 18 to 24 hours later. elevated temperatures, heart and respiratory rates were constant features. the animals were severely leucopaenic, and showed an absolute neutropaenia. at autopsy the lungs were enlarged, and showed varying degr ... | 1978 | 210751 |
| natural intra-uterine infection of foals with equine herpes virus type 1 in victoria. | | 1978 | 210752 |
| equine abortion associated with herpesvirus. | | 1978 | 210753 |
| cobalt exchange in horse liver alcohol dehydrogenase. | the preparation of metal hybrid species of horse liver alcohol dehydrogenase is made possible by the development of carefully delineated systems of metal in equilibrium metal exchange employing equilibrium dialysis. the conditions which are optimal for the site-specific replacement of the catalytic and/or noncatalytic zinc atoms of the native enzyme by cobalt are not identical with those which are utilized for substitution with 65zn. thus, while certain 65zn hybrids can be prepared by exploiting ... | 1978 | 210784 |
| the electric potential field around cytochrome c and the effect of ionic strength on reaction rates of horse cytochrome c. | 1. the electric potential fields around tuna ferri- and ferrocytochrome c were calculated assuming that (i) all of the lysines and arginines are protonated, (ii) all of the glutamic and aspartic acids and the terminal carboxylic acid are dissociated, and (iii) the haem has a net charge of +1e in the oxidized form. 2. near the haem crevice high values for the potential (greater than +2.5 kt/e) are found. consequently, electron transfer via the haem edge is favored if the oxidant or reductant is n ... | 1978 | 210807 |
| the effects of alpha and beta adrenergic agents on spleen cell antigen binding in four amphibian species. | adults of two urodele amphibian species (triturus cristatus carnifex and cynops hongkongensis) and two anuran species (rana temporaria and xenopus laevis laevis) were immunized with a 25% suspension of sheep or horse erythrocytes. after eight or 14 days, splenic lymphocytes were removed, and their specific red cell-binding capacities tested by immunocytoadherence. antigen-binding cells were classified as high-dose nonsecretory (s-) or secretory (s+), according to whether they bound a single laye ... | 1978 | 211039 |
| enterotoxemia in two foals. | two quarter horse foals from different premises died from enterotoxemia. clostridium perfringens toxins alpha and beta were demonstrated in the foal's intestines by mouse protection tests. clostridium perfringens type c was isolated from the intestines of each foal. histologic examination revealed hemorrhage, necrosis, and massive numbers of c perfringens. | 1978 | 211108 |
| photochemical inactivation of dna and rna viruses by psoralen derivatives. | western equine encephalitis virus, and rna virus, and herpes simplex virus type i, a dna virus, were efficiently inactivated in less than i min by exposure to long-wave ultraviolet light (320 to 380 nm) in the presence of several psoralen derivatives. the psoralen photochemical reaction was chosen for study due to its known specificity for nucleic acids. neither the light nor any of the drugs alone caused appreciable inactivation. the inactivation kinetics and dependence on light intensity and o ... | 1978 | 211184 |
| [outbreak of equine influenza caused by influenza virus a/equi 1 in the state of rio de janeiro, brazil]. | | 1978 | 211560 |
| [enzymatic and ultrastructural changes in isolated liver mitochondria from mice infected with several group a arboviruses]. | inoculation of white mice of varying body mass with pathologic strains of eastern and venezuelan equine encephalomyelitis viruses and their attenuated variants (dnc-20/6 and no. 2621), promising as vaccine candidates, resulted in an increase of enzymatic activity and ultrastructural changes of isolated mitochondria from livers of the animals. the attenuated strains of the viruses were shown to induce temporary changes in both aspects of the study which became normal by the end of the study. ther ... | 1978 | 211733 |
| skin hypersensitivity to equid herpesvirus type 1 in horses. | | 1978 | 211756 |
| spin contribution to the thermodynamics of ligand binding to methemoglobins and metmyoglobins. i. azide ion binding to horse-heart myoglobin. | | 1978 | 212134 |
| [increase in the antiviral resistance of cells after treating them with messenger rna for antiviral protein]. | the authors studied antiviral resistance of murine cells l-929 following single introduction into them of homologous and heterologous informative rna preparations for antiviral protein (i-rna-avp). as shown, in using homologous i-rva-avp preparations suppression of the virus production constituted 90--93%, and was stably traced in cell passage in the course of 1 1/2 months (observation period). following cell contact with heterologous i-rna-avp preparations suppression the first 6 passages of th ... | 1978 | 212137 |
| electron microscopy in the diagnosis of infectious diarrhea. | | 1978 | 212395 |
| biochemical transformation of deoxythymidine kinase-deficient mouse cells with uv-irradiated equine herpesvirus type 1. | a line of 3t3 mouse cells lacking deoxythymidine kinase (dtk-) was stably transformed to the dtk+ phenotype after exposure to uv-irradiated equine herpesvirus type 1 (ehv-1). biochemical transformants were isolated in a system selective for the dtk+ phenotype (eagle minimal essential medium containing 10(-4) m hypoxanthine, 6 x 10(-7) m aminopterin, and 2 x 10(-5) m deoxythymidine). transformation was accompanied by the acquisition of a dtk activity with immunological, electrophoretic, and bioch ... | 1978 | 212607 |
| a serologic survey on equine influenza for the past ten years. | | 1978 | 212632 |
| adenoviral infection of arab foals with respiratory tract disease. | | 1978 | 212906 |
| effect of premenopausal castration and incremental dosages of conjugated equine estrogens on plasma follicle-stimulating hormone, luteinizing hormone, and estradiol. | plasma levels of follicle-stimulating hormone (fsh), luteinizing hormone (lh), and estradiol (e2) were measured serially in 11 premenopausal patients before and after hysterectomy with bilateral salpingo-oophorectomy. one week after operation an incremental dosage regimen of conjugated estrogens (cee) in tablet form was commenced on a basis of two weeks with therapy (0.3, 0.625, 1.25, and finally 2.5 mg.), with each dose interspersed by two weeks without therapy. fsh, lh and e2 levels were measu ... | 1978 | 212949 |
| results of an epidemiological investigation on viral arteritis in france and some other european and african countries. | an original microplate seroneutralization technique was developed in order to study the existence of antibodies against equine viral arteritis. the technique involves a high amount of complement, and has allowed to demonstrate antibodies in 18.5% out of 4,037 horses examined. titers varied not or little during periods as long as 6 years. among 3,324 sera samples from french horses, a 15.2% frequency of antibodies was shown. the infection level was not very different between breeds, which is diff ... | 1978 | 213008 |
| rotavirus diarrhoea of foals. | | 1978 | 213048 |
| evolutionary change of the heme c electronic structure: ferricytochrome c-551 from pseudomonas aeruginosa and horse heart ferricytochrome c. | | 1978 | 213063 |
| fluorescence quenching and energy transfer in complexes between horse-liver alcohol dehydrogenase and coenzymes. | | 1978 | 213266 |
| puberty-related alterations in the organization of sleep--wakefulness states: differences between spontaneous and induced pubertal conditions. | | 1978 | 213302 |
| concurrent rotavirus and salmonella infections in foals. | | 1978 | 213413 |
| [purification of equine influenza virus a/equi-2/w/9/69 by the sucrose step density gradient ultracentrifugation]. | | 1978 | 213662 |
| pathogenicity and immunologic studies of equine adenovirus in specific-pathogen-free foals. | | 1978 | 213995 |
| common membrane neoantigens on bovine papilloma virus-induced fibroma cells from cattle and horses. | cultured cells from bovine papilloma virus (bpv)-induced fibroblastic tumors and normal dermis of cattle, horses, and hamsters were examined for cell membrane or internal neoantigens, using the indirect immunofluorescence technique. sera from cattle and horses bearing bpv-induced fibromas cross reacted with cell membranes of tumor, but not with normal dermal cells of both species. the reaction could be blocked with homologous, but not heterologous, serum of these 2 species. immunofluorescence wa ... | 1978 | 213996 |
| modulation of adenylate cyclase activity by sulfated glycosaminoglycans. i. inhibition by heparin of gonadotrophin-stimulated ovarian adenylate cyclase. | heparin inhibits (i50 = 2 microgram/ml) the activity of luteinizing hormone and human chorionic gonadotropin-stimulated adenylate cyclase in purified rat ovarian plasma membranes. unstimulated enzyme activity and activity stimulated by naf, gtp or guanosine 5'-(beta,gamma-imido)triphosphate were inhibited to a lesser extent. human chorionic gonadotropin binding to this membrane preparation was inhibited by heparin (i50 = 6 microgram/ml). the inhibition with respect to hormone concentration was o ... | 1978 | 214154 |
| induction of deoxythymidine kinase activity in several mammalian cell lines after infection with six different strains of equine herpesvirus type 1 (ehv-1). | | 1978 | 214953 |
| scanning and transmission electron microscopic study of equine infectious anemia virus. | scanning and transmission electron microscopy were used to study in detail the morphogenesis and replication of equine infectious anemia virus (eiav) in cultured, persistently infected equine fetal kidney fibroblasts. the eiav was shown by thin-section electron microscopy to resemble morphologically more closely the members of the genus lenti-virus in the family retroviridae than other genera. scanning electron microscopy demonstrated budding virus on only about 5% of the equine fetal kidney fib ... | 1978 | 215061 |
| equine rhinopneumonitis vaccine: immunogenicity and safety in foals. | | 1978 | 215062 |