| improvement of the tissue integration of a new modified polytetrafluoroethylene prosthesis: mycro mesh. | we studied the behaviour of the different tissue interfaces formed on a new type of prosthesis used for the repair of abdominal wall defects, mycro mesh (w. l. gore and ass., flagstaff, az, usa), which consists of perforated layers of polytetrafluoroethylene (ptfe). in 20 new zealand white rabbits, a full-thickness (except skin) 7 cm x 5 cm defect was created in the anterior abdominal wall. the defects were repaired with a prosthetic implant (mycro mesh) that was placed in direct contact with ab ... | 1996 | 8805973 |
| hypertrophic osteopathy (marie's disease) in equidae: a review of twenty-four cases. | the case records of 23 horses and one donkey affected by hypertrophic osteopathy (ho) (marie's disease) were reviewed. all affected animals presented with limb swellings, which were bilaterally symmetrical and usually involved both fore- and hindlimbs. associated signs included stiffness/lameness and weight loss. radiological features included periosteal new bone formation over the diaphyses and metaphyses of affected bones. the metacarpal and metatarsal bones were most frequently affected. arti ... | 1996 | 8818590 |
| inhibition of coagulation factors by recombinant barley serpin bszx. | barley serpin bszx is a potent inhibitor of trypsin and chymotrypsin at overlapping reactive sites (dahl, s.w., rasmussen, s.k. and hejgaard, j. (1996) j. biol. chem., in press). we have now investigated the interactions of bszx with a range of serine proteinases from human plasma, pancreas and leukocytes, a fungal trypsin and three subtilisins. thrombin, plasma kallikrein, factor viia/tissue factor and factor xa were inhibited by bszx at heparin independent association rates (k(ass)) of 4.5 x 1 ... | 1996 | 8843156 |
| efficacy of triclabendazole against fasciola hepatica in the donkey (equus asinus). | | 1996 | 8863404 |
| the mitochondrial dna molecule of sumatran orangutan and a molecular proposal for two (bornean and sumatran) species of orangutan. | the complete mitochondrial dna (mtdna) molecule of sumatran orangutan, plus the complete mitochondrial control region of another sumatran specimen and the control regions and five protein-coding genes of two specimens of bornean orangutan were sequenced and compared with a previously reported complete mtdna of bornean orangutan. the two orangutans are presently separated at the subspecies level. comparison with five different species pairs-namely, harbor seal/grey seal, horse/donkey, fin whale/b ... | 1996 | 8875856 |
| the complete mitochondrial dna (mtdna) of the donkey and mtdna comparisons among four closely related mammalian species-pairs. | the nucleotide sequence of the complete mitochondrial genome of the donkey, equus asinus, was determined. the length of the molecule is 16,670 bp. the length, however, is not absolute due to pronounced heteroplasmy caused by variable numbers of two types of repetitive motifs in the control region. the sequence of the repeats is (a) 5'-cacaccca and (b) 5'-tgcgcgca, respectively. the order of (a) and (b) can be expressed as {n[2(a)+(b)]+m(a)}. in 32 different clones analyzed the number of n and m ... | 1996 | 8875857 |
| synteny mapping in river buffalo. | the cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of southern hybridization technique. five syntenic groups, tcrb-pgy3, ass-abl, fuca1p-cryg, mbp-yes1, and cgn1-acta1, previously assigned to cattle as u13, u16, u17, u28, and u29 respectively, were also found to be syntenic in buffalo. based on the extensive syntenic conservation and banding homology between cattl ... | 1996 | 8875891 |
| a sequence similarity search algorithm based on a probabilistic interpretation of an alignment scoring system. | we present a probabilistic interpretation of local sequence alignment methods where the alignment scoring system (ass) plays the role of a stochastic process defining a probability distribution over all sequence pairs. an explicit algorithms is given to compute the probability of two sequences given and ass. based on this definition, a modified version of the smith-waterman local similarity search algorithm has been devised, which assesses sequence relationships by log likelihood ratios. when te ... | 1996 | 8877503 |
| nationwide serological survey of equine influenza in nigeria. | the objective of this work was to examine the incidence of equine influenza viruses in the equine population of an area of tropical africa where equine influenza virus activity has recently been reported for the first time. a serological survey of sera from horses and donkeys from regions of nigeria taken from 1990 to 1993 was carried out and the results obtained were com-pared with equine sera from western europe (ireland). the sera were assayed for presence of antibodies by both haemagglutinat ... | 1996 | 8881415 |
| [prevalence of rhinoestrus usbekistanicus (gan 1947) in donkeys (equus asinus) in senegal]. | a survey performed in dakar (senegal) showed, for the first time, the presence of rhinoestrus usbekistanicus in donkeys in the south of the sahara desert. out of the 138 heads examined between november and june, 84% were infected by a mean burden of 15.9 larvae (9.7 l1, 1.8 l2 and 4.1 l3). there is a hypobiotic period starting in march during the dry season. | 1996 | 8881418 |
| a radioimmunoassay for the determination of tipredane in human plasma. | a sensitive method for the determination of tipredane in human plasma has been developed. prior to radioimmunoassay, proteins are removed from plasma by precipitation with acetonitrile. a [125i]iodohistamide derivative of tipredane is used as a heterogeneous radioligand. the primary antiserum is raised in sheep against a tipredane (o-carboxymethyl) oxime-bovine serum albumin conjugate. donkey anti-sheep igg antiserum is used as the secondary antiserum in a double antibody separation procedure. t ... | 1996 | 8895073 |
| the complete mitochondrial dna sequence of the greater indian rhinoceros, rhinoceros unicornis, and the phylogenetic relationship among carnivora, perissodactyla, and artiodactyla (+ cetacea). | the sequence (16,829 nt) of the complete mitochondrial genome of the greater indian rhinoceros, rhinoceros unicornis, was determined. like other perissodactyls studied (horse and donkey) the rhinoceros demonstrates length variation (heteroplasmy) associated with different numbers of repetitive motifs in the control region. the 16,829-nt variety of the molecule includes 36 identical control region motifs. the evolution of individual peptide-coding genes was examined by comparison with a distantly ... | 1996 | 8896369 |
| don't dissect use the disector! 'if you assume, you can make an ass out of u and me': a decade of the disector for sterological counting of particles in 3d space. | | 1996 | 8906439 |
| comparative morphology of the pectinate ligaments of domestic mammals, as observed under the dissecting microscope and the scanning electron microscope. | the pectinate ligaments of ten horses, two donkeys, five oxen, five sheep, ten goats, five dogs, five cats, thirty pigs and two rabbits were studied under the stereomicroscope and the scanning electron microscope. in the horse and the donkey, the pectinate ligament was very prominent and was characterized by sturdy interconnected strands and relatively small intertrabecular spaces. the pectinate ligaments of ruminants were composed of shorter strands, separated by relatively larger spaces. fusio ... | 1996 | 8915997 |
| transmission of the south african asinine strain of equine arteritis virus (eav) among horses and between donkeys and horses. | lateral and sexual transmission of eav among horses and lateral transmission between donkeys and horses were attempted by experimental infection with the south african asinine strain. clinical, immunological and virological responses were evaluated. all intramuscularly inoculated horses developed very mild clinical signs, were viraemic, shed virus from nasopharynx, and seroconverted. lateral infection was demonstrated in one in-contact mare. reinfection of two stallions by intranasal instillatio ... | 1996 | 8917855 |
| heterophile antibodies to chicken erythrocytes in sheep infected with trypanosoma congolense. | high hemagglutinin titres against chicken erythrocytes were detected in the sera of trypanosoma congolense-infected sheep. adsorption of sheep sera with solubilized t. congolense resulted in marked reduction of hemagglutinin titre. heat inactivation of the sera at 56 degrees c for 30 min had no demonstrable effects on the hemagglutinin titre. sera collected from the sheep before trypanosome infection did not agglutinate chicken erythrocytes. on the other hand, erythrocytes of horse, donkey and d ... | 1996 | 8917863 |
| analysis of the long terminal repeat from a cytopathic strain of equine infectious anemia virus. | sequential passage of the tissue culture-adapted prototype strain of eiav in fetal donkey dermal (fdd) cell cultures generated a virus stock which exhibits cytopathic effects in fdd cell cultures. in this study, the effects of the long terminal repeat (ltr) region on virus replication and cytopathogenicity were examined. the fdd-adapted virus ltr was found to contain a number of base pair mutations and a large insertion within the u3 region in comparison with the previously characterized ltr, la ... | 1996 | 8918926 |
| prevalence of sarcocysts in livestock of northwest ethiopia. | a survey of sarcocystis was conducted in cattle, sheep, goats, donkeys and chickens. a total of 671 haematoxylin-eosin (h-e) stained muscle tissue samples, including diaphragm, masseter, cardiac and oesophageal musculatures were examined. additionally, cardiac muscle samples from 40 fetuses were included. an infestation rate of 93% in sheep, 82% in cattle, 81% in goats, 16.6% in donkeys and 6.6% in chickens was noted. the infestation rate of diaphragm, masseter, cardiac and oesophageal musculatu ... | 1996 | 8919969 |
| pharmacokinetics of ketoprofen in the donkey (equus asinus). | the pharmacokinetic parameters of ketoprofen were determined in four donkeys after a single intravenous injection of a dose of 2.2 mg/kg body weight. the total body clearance (clb) was 414.0 +/- 98.70 ml/h/kg (mean +/- sd), the volume of distribution at steady state (vss) 263.10 +/- 55.43 ml/kg and the elimination half-life 1.30 +/- 0.75 h. these values were compared to those obtained in horses. | 1996 | 8921728 |
| alterations in glomerular anionic sites in the autologous phase of canine anti-glomerular basement membrane nephritis. | there have been a few studies on canine nephrotoxic glomerulonephritis produced by anti-glomerular basement membrane serum (agbm), but these reports have not focused on an alteration in the charge properties of glomerular basement membrane (gbm). in this study, rabbit agbm or normal rabbit serum (nrs) was given intravenously (2 ml/kg body weight) to 16 male beagle dogs. an alteration of anionic sites (ass) of gbm was studied quantitatively using polyethyleneimine as a cationic probe by electron ... | 1996 | 8923685 |
| [preparation and study of recombinant strains of venezuelan equine encephalomyelitis virus expressing the pres2-s gene of the hepatitis b virus]. | venezuelan equine encephalomyelitis (vee) virus-based vectors for expression of heterologous genes have been constructed using full-length cdna copy of the interstrain recombinant virus (trinidad donkey and 230 strain). gene cassettes carrying the subgenomic mrna promoter of vee virus and the pres2-s gene of hepatitis b virus (hbv) were inserted before or after the genes of structural viral proteins. live virus stocks were obtained by transfection of chick embryo fibroblasts with in vitro transc ... | 1996 | 8928499 |
| anisoosmotic regulation of hepatic gene expression. | the effect of anisoosmolarity on the abundance of various mrna species was examined in perfused rat liver and h4iie rat hepatoma cells. hyperosmotic exposure (385 mosmol/l) of isolated rat livers increased mrna levels for tyrosine aminotransferase (tat) by 246% and those for phosphoenolpyruvate carboxykinase (pepck) by 186%, whereas hypoosmotic exposure (225 mosmol/l) decreased their levels to 43% and 42%, respectively. mrna levels for fructose-1,6-bisphosphatase (fbp), argininosuccinate lyase ( ... | 1996 | 8929814 |
| smooth-pursuit eye movement and saccadic intrusions in obsessive-compulsive disorder. | although several reports agree that smooth-pursuit eye movement (spem) is abnormal in some obsessive-compulsive disordered (ocd) patients, differences between treatments and lack of accuracy in control selection make the results controversial. although reduced gain seems the most accepted abnormality, the characteristics of saccadic disruption of smooth pursuit are as yet unspecified. spems in 21 ocd patients (dsm-iii-r) and 21 healthy subjects recruited from the community were studied through a ... | 1996 | 8931920 |
| essential thrombocythemia and pregnancy. | a review of the literature disclosed 106 pregnancies (preg.) in 57 women with essential thrombocythemia (et). the success rate (baby alive) was 57% (60 live births/106 preg.), the rate of miscarriage 43% (46 miscarriages/106 preg.). the most frequent complication was spontaneous abortion during the first trimester in 36% (38 abortions/106 preg.). other complications such as intrauterine death and stillbirth after the 28th week, which occurred in 5% (7/106), premature delivery in 8% (8/106), pre- ... | 1996 | 8951773 |
| use of donkeys and their draught performance in smallholder farming in zimbabwe. | animal traction constitutes the most important source of power for agricultural work in smallholder farming in zimbabwe. two studies, a survey and a short term on-farm trial were conducted to evaluate the use of donkeys as draught animals. the survey covered 59 households in 2 smallholder farming areas. for the on-farm trial, 12 donkeys and 12 cattle were spanned separately in teams of 4 animals to plough 40 m x 70 m plots of medium textured soil. the survey findings highlighted the drought tole ... | 1995 | 8966767 |
| endothelial cell dysfunction secondary to the adhesion of diabetic erythrocytes. modulation by iloprost. | we previously showed the correlation between the extent of vascular complications and erythrocyte adherence to endothelium in diabetes mellitus. the accumulation of advanced glycation end products (ages) on the erythrocyte surface in diabetes mediates their interaction with endothelial cells through a specific endothelial receptor for ages (rage). binding of diabetic erythrocytes to endothelial cells resulted in evidence of oxidant stress responsible for a range of cellular perturbations. in the ... | 1996 | 8971575 |
| kinetics of binding of antp homeodomain to dna analyzed by measurements of surface plasmon resonance. | the kinetics of binding of the antp homeodomain to three kinds of dna fragment were analyzed by measurements of surface plasmon resonance at various temperatures. non-specific and specific binding of the homeodomain to dna was examined. in the case of non-specific binding, the association rate constant (k(ass)) was estimated to be 1.41-2.62 x 10(5) m(-1) s(-1) and the dissociation rate constant (k(diss)) was 1.36-3.10 x 10(-2) s(-1), thus, the dissociation constant (kd) was 0.847-1.72 x 10(-7) m ... | 1996 | 8977123 |
| applications of pig zona pellucida immunocontraception to wildlife fertility control. | a unique application of pig zona pellucida (pzp) immunocontraception is the control of wildlife populations. a native pzp vaccine has been successfully applied to wild horse and donkey populations. a single annual booster inoculation was capable of maintaining contraception. seven consecutive years of pzp treatment in wild mares resulted in no detectable debilitating side effects, and reversibility of contraception has been documented among mares treated for up to 4 consecutive years. long-term ... | 1996 | 8984182 |
| long-chain fatty acids suppress the induction of urea cycle enzyme genes by glucocorticoid action. | in order to test the possibility that free fatty acids are the mediator of the abnormal expression of urea cycle enzyme genes in carnitine-deficient juvenile visceral steatosis (jvs) mice, the effects of fatty acids on urea cycle enzyme, carbamoylphosphate synthetase (cps) and argininosuccinate synthetase (ass), mrna levels were examined in rat primary cultured hepatocytes. addition of a synthetic glucocorticoid hormone, dexamethasone, caused increases in cps and ass mrnas. further addition of o ... | 1996 | 8985169 |
| observations on the prevalence of trypanosomosis in small ruminants, equines and cattle, in relation to tsetse challenge, in the gambia. | the prevalence of trypanosome infections in djallonké sheep and west african dwarf goats at different sites in the gambia showed a significant, positive correlation with contemporary assessments of tsetse challenge. a similar correlation was observed in village n'dama cattle which showed comparable prevalence values in the same areas. trypanosome prevalences also tended to be higher in horses and donkeys in areas with high tsetse challenge compared with sites with relatively few flies. a ranking ... | 1996 | 8988551 |
| comparative pharmacokinetics of phenylbutazone and its metabolite oxyphenbutazone in clinically normal horses and donkeys. | to compare plasma disposition of phenylbutazone and its metabolite oxyphenbutazone after i.v. administration of phenylbutazone in horses and donkeys. | 1997 | 8989496 |
| [alpha 2-adrenoreceptor agonists--horse or donkey?]. | | 1996 | 8991465 |
| clostridium pascui sp. nov., a new glutamate-fermenting sporeformer from a pasture in pakistan. | four strains of an obligately anaerobic spore-forming bacterium were isolated from soil samples from a donkey pasture in pakistan. comparative 16s rrna sequence analysis demonstrated that the strains are members of phylogenetic cluster i of the genus clostridium (collins et al. 1994). the strains are mesophilic, nonsaccharolytic, and nonproteolytic, utilize glutamate and histidine, and produce indole. acetate, butyrate, ethanol, hydrogen, and carbon dioxide are the products of fermentation. alth ... | 1997 | 8995820 |
| [endoparasites of donkeys and horses kept in communal housing in upper bavaria; species spectrum and incidence]. | in this epidemiological study of endoparasites 37 donkeys and 23 horses were included, which were examined coproscopically in regular intervals over a 15 month period. the animals derived from ten farms, in which regular prophylactic treatments with anthelmintics had been practiced at least for two years before this investigation. this study revealed the presence of at least twelve parasite species in donkeys, eimeria leuckarti, gasterophilus intestinalis, fasciola hepatica, anoplocephala perfol ... | 1996 | 8999593 |
| nosology of fetal hypokinesia sequence based on cns abnormalities: is there an aase-smith syndrome? | aase-smith syndrome (ass) is usually defined as a dominantly inherited combination of arthrogryposis, dandy-walker malformation and cleft palate. we describe a sporadic case of foetal akinesia with abnormal fossa posterior, fitting the diagnosis of ass, and discuss the nosology of this entity among syndromes with distal arthrogryposis. ass shows a "hybrid" phenotype: adults with mild ass could be classified as distal arthrogryposis, whereas severely affected newborns overlap with the marden-walk ... | 1996 | 9001811 |
| evidence for embryonic prostaglandin h synthase-catalyzed bioactivation and reactive oxygen species-mediated oxidation of cellular macromolecules in phenytoin and benzo[a]pyrene teratogenesis. | a mouse embryo culture model was used to determine whether embryonic prostaglandin h synthase (phs)-catalyzed bioactivation and resultant oxidative damage to embryonic protein and dna may constitute a molecular mechanism mediating phenytoin and benzo[a]pyrene teratogenesis. embryos were explanted from cd-1 mouse dams on gestational day 9.5 (vaginal plug = day 1) and incubated for either 4 h (biochemistry) or 24 h (embryotoxicity) at 37 degrees c in medium containing either phenytoin (20 microgra ... | 1997 | 9013124 |
| a fragment of the major histocompatibility complex class ii-associated p41 invariant chain inhibits cruzipain, the major cysteine proteinase from trypanosoma cruzi. | a peptide fragment derived from the p41 form of the invariant chain (ii) associated with the major histocompatibility complex (mhc) class ii molecule has been shown to inhibit the mammalian lysosomal cysteine proteinase, cathepsin l, and to be a novel cysteine proteinase inhibitor, distinct from cystatins. here we report that this same fragment also binds to and inhibits cruzipain, the cathepsin l-like enzyme from the protozoan parasite trypanosoma cruzi. the binding of the ii fragment to cruzip ... | 1997 | 9013899 |
| [jenner's cowpox vaccine in light of current vaccinology]. | two hundred years ago edward jenner inoculated james phipps with vaccinia and 181 years later smallpox had disappeared from the surface of the earth as a result of generalized vaccination. compared to the requirements of modern vaccinology, the procedures used by jenner and his successors, were extremely primitive because of an almost total lack of knowledge in the field of microbiology and immunology. the active principle of smallpox vaccine is vaccinia virus, which in many respects, differs fr ... | 1996 | 9027132 |
| a survey for antibodies to equine arteritis virus in donkeys, mules and zebra using virus neutralisation (vn) and enzyme linked immunosorbent assay (elisa). | a seroepidemiological survey of donkeys in south africa (n = 4300) indicated a wide distribution and increasing prevalence of antibodies to equine arteritis virus (eav). donkey sera inhibited equine arteritis virus infection in virus neutralisation (vn) tests and in elisa specifically bound to a recombinant antigen derived from the bucyrus isolate of eav. these results suggest that donkeys have been exposed to the same serotype of this virus as circulates among horses. a good correlation existed ... | 1997 | 9031862 |
| suppressed expression of the urea cycle enzyme genes in the liver of carnitine-deficient juvenile visceral steatosis (jvs) mice in infancy and during starvation in adulthood. | systemic carnitine-deficient juvenile visceral steatosis (jvs) mice exhibit decreased expression of some liver-selective genes including those for the urea cycle enzymes during the infantile period. at 25 days, carbamoylphosphate synthetase (cps) mrna level was remarkably low in the liver of jvs mice, and the hnf-4 and c/ebp-alpha mrna contents were also reduced. hnf-3 alpha and c/ebp-beta mrnas were slightly higher in the liver of jvs mice, and hnf-1 mrna remained normal. these results, togethe ... | 1997 | 9058209 |
| expression of horse and donkey lh in cos-7 cells: evidence for low fsh activity in donkey lh compared with horse lh. | horse (equus caballus) luteinizing hormone (elh) and chorionic gonadotrophin (ecg), which have the same amino acid sequence, are unusual in that, although they express only lh activity in equids, they express dual lh and fsh activities in all other species tested. donkey (equus asinus) lh (dklh) and cg (dkcg), which also share an identical peptide backbone, have been less well characterized and conflicting results concerning their fsh activity in heterologous species have appeared in the literat ... | 1997 | 9071957 |
| placental localization of relaxin in the pregnant mare. | in situ hybridization employing a crna probe derived from a 428-bp fragment of equine relaxin was used to localize relaxin mrna, and immunocytochemistry was used to localize relaxin itself, in tissues of the placenta-endometrium interface recovered between 33 and 153 days of gestation from mares carrying intraspecific horse, interspecific mule and extraspecific donkey conceptuses. immunocytochemical staining was also used to localize trophoblast-specific and class i major histocompatibility comp ... | 1997 | 9089772 |
| role and management of donkeys in samburu and turkana pastoralist societies in northern kenya. | a survey was carried out in northern kenya to obtain data on the role and management of donkeys in samburu and turkana pastoralist societies and to identify potential areas, if any, for interventions. sixty-seven women owning or closely associated with 583 donkeys were surveyed during july/august 1994. the median donkey herd sizes in samburu and turkana societies were 4 and 10 respectively. pack transport of water and domestic materials were the major reasons for keeping donkeys. donkey milk, me ... | 1997 | 9090018 |
| mutations and dna diagnoses of classical citrullinemia. | classical citrullinemia is an autosomal recessive disease caused by a genetic deficiency of argininosuccinate synthetase (ass). we have previously identified 20 mutations in ass mrna of human classical citrullinemia and already established the dna diagnosis of seven mutations as follows. by southern blot analysis, each of the alleles with exon 5 or 6 deletion in mrna appears to involve deletion of genomic dna from this region. five mutations involving r304w, g324s, ivs-6(-2) (delta ex7), ivs-13( ... | 1997 | 9090528 |
| [superselective spinal anesthesia for saphenectomy intervention. comparison of 2 concentrations of hyperbaric bupivacaine]. | high concentrations of intrathecal local anaesthetics may be neurotoxic. the purpose of this study was to compare the features of superselective spinal anaesthesia (ass) obtained with an equivalent dose (5 mg) of 0.5% (available now also in italy) and 1% hyperbaric bupivacaine. | 1996 | 9102589 |
| wound infection caused by staphylococcus hyicus subspecies hyicus after a donkey bite. | staphylococcus hyicus subspecies hyicus has not previously been reported to cause human infections, but is a well known cause of diseases in a variety of animals. we report a wound infection following a donkey bite. | 1997 | 9112308 |
| apoptotic cell death is an important cause of neuronal injury in experimental venezuelan equine encephalitis virus infection of mice. | mice develop a fatal encephalomyelitis after infection with the trinidad donkey strain of venezuelan equine encephalitis (vee) virus. adult mice were inoculated intraperitoneally with vee virus and the brains were examined at different time points. morphological changes were assessed by histological staining. vee virus antigen was detected with immunoperoxidase staining, and dna fragmentation was evaluated in situ using the terminal deoxynucleotidyl transferase-mediated dutp-digoxigenin nick end ... | 1997 | 9113200 |
| field trial evaluation of detomidine as a sedative and analgesic in horses with colic. | in this uncontrolled clinical study 12 investigators cooperated to evaluate the analgesic and sedative effect of detomidine (dormosedan; farmos group ltd; finland) in 234 horses with abdominal pain caused by colic. the study was designed to use each animal as its own control and to evaluate its response to the drug over a 60 min period. detomidine was given intravenously (i.v.) once in 169 cases (167 horses, 1 mule, 1 donkey) at a dose of 20 micrograms/kg bodyweight (bwt), and to 65 horses at 40 ... | 1989 | 9118092 |
| clinical and molecular genetic characterisation of a family segregating autosomal dominant retinitis pigmentosa and sensorineural deafness. | to characterise clinically a large kindred segregating retinitis pigmentosa and sensorineural hearing impairment in an autosomal dominant pattern and perform genetic linkage studies in this family. extensive linkage analysis in this family had previously excluded the majority of loci shown to be involved in the aetiologies of rp, some other forms of inherited retinal degeneration, and inherited deafness. | 1997 | 9135384 |
| [immunopathology of borna disease in the horse: clinical, virological and neuropathologic findings]. | tissues from nine horses and one donkey suffering from natural borna disease were investigated. clinically, all animals demonstrated progressive reduced mentation and aggravating gait disturbances. during the clinical course anorexia and progressive loss of proprioception were observed. cranial nerve failure was accompanied by signs of pharyngeal paralysis, sialorrhea, bruxism, and by blindness. virologically, infectious virus was detected in the brain of all animals investigated but was not fou ... | 1996 | 9139422 |
| pancreatic secretory trypsin inhibitor as a diagnostic marker for adult-onset type ii citrullinemia. | adult-onset type ii citrullinemia is characterized by which deficiency of argininosuccinate synthetase (ass) protein is found specifically in the liver of patients. our recent study using differential messenger rna (mrna) display showed that the expression of human pancreatic secretory trypsin inhibitor (hpsti) mrna increases significantly in the liver of all type ii patients tested. in the present work, we found that the concentration of hpsti protein was higher in the liver of type ii patients ... | 1997 | 9141434 |
| fine localization of the torsion dystonia gene (dyt1) on human chromosome 9q34: yac map and linkage disequilibrium. | the dyt1 gene, which maps to chromosome 9q34, appears to be responsible for most cases of early-onset torsion dystonia in both ashkenazic jewish (aj) and non-jewish families. this disease is inherited in an autosomal dominant mode with reduced penetrance (30%-40%). the abnormal involuntary movements associated with this disease are believed to be caused by unbalanced neural transmission in the basal ganglia. previous linkage disequilibrium studies in the aj population placed the dyt1 gene in a 2 ... | 1997 | 9149944 |
| localization of regulatory peptides in the male urogenital apparatus of domestic equidae: a comparative immunohistochemical study in equus caballus and equus asinus. | an immunohistochemical study was carried out on specimens of testis, excurrent duct including the male accessory glands and urethra in its various tracts in the horse and the donkey, in order to localize nine regulatory peptides. immunoreactivities were tested by means of labelled strept avidin-biotin (lsab) method. the study has shown that equine male genitalia are supplied by many peptide immunoreactive nerves containing npy-, vip-, leu- and met-enkephalin-, substance p-, cgrp- and bombesin/gr ... | 1997 | 9151118 |
| differences in a ribosomal dna sequence of lungworm species (nematoda:dictyocaulidae) from fallow deer, cattle, sheep and donkeys. | the second internal transcribed spacer (its2)* of the ribosomal dna of dictyocaulus viviparus from cattle, d eckerti from fallow deer. d filaria from sheep and d arnfieldi from donkeys has been sequenced to investigate the genetic relationships among lungworm species, especially between d viviparus and d eckerti, because the latter is not generally accepted as a separate species. the length of the its2 varied between 403 and 481 bases, and its gc content ranged from 25 to 33 per cent. intraspeci ... | 1997 | 9160418 |
| simulation studies of vaccination strategies in african horse sickness. | a simulation model including two hosts (horses and donkeys) and one vector (culicoides imicola) for african horse sickness in spain is extended to consider vaccination strategies. if hosts were protected prior to virus introduction, elimination of simulated epidemics was related nonlinearly to the fraction protected. protecting donkeys as well as horses increased the effectiveness of vaccination. prevention of 50% of epidemics required 75% coverage of horses and donkeys or 90% coverage of horses ... | 1997 | 9160519 |
| glucocorticoids play an important role in mediating the enhanced metabolism of arginine and glutamine in enterocytes of postweaning pigs. | weaning is associated with increased intestinal metabolism of glutamine and arginine as well as elevated plasma concentrations of cortisol (the major circulating glucocorticoid) in pigs. the objective of this study was to determine if cortisol plays an important role in mediating the enhanced amino acid metabolism in enterocytes of weaned pigs by administering ru486 (a glucocorticoid receptor antagonist). eighteen 21-d-old pigs were randomly assigned to three groups of six. two of these groups r ... | 1997 | 9164994 |
| [secondary prevention of ischemic strokes: effect of dosage of aspirin]. | the value of acetylsalicylic acid (ass) in the secondary prevention of ischemic stroke is well established. however, the optimum dose of aas for stroke-threatened patients remains unsettled. this paper reviews the pattern of adverse reactions to aas and their relationship to the dosage of ass evaluated. | 1997 | 9172913 |
| the effect of ammonium chloride on hepatic and renal metabolism in the rat. | the metabolic effects of an ammonium salt on the liver and kidney were investigated. rats were allowed free access to a 0.28 m ammonium chloride (nh4cl) solution for 7- and 8-day periods. serum urea concentration was significantly increased after 8 days of nh4cl ingestion. however the following hepatic urea cycle enzymes remained unchanged: cps, otc, ass and asl. the pattern of urinary urea excretion was variable. when the data for the 7-day period were pooled, there was no significant differenc ... | 1997 | 9192912 |
| cloning, sequencing and in vitro functional expression of recombinant donkey follicle-stimulating hormone receptor: a new insight into the binding specificity of gonadotrophin receptors. | among all mammalian fsh receptors (fsh-r; including donkey (dk) fsh-r), only horse (hs) fsh-r does not bind hslh/chorionic gonadotrophin (cg). in order to delineate the structural origin of hsfsh-r specificity precisely, we have cloned dkfsh-r cdna from donkey testis mrna by rt-pcr. transiently expressed dkfsh-r endowed cos-7 cells with both hslh/cg- and fsh-binding activity, as well as fsh-induced camp production. the deduced dkfsh-r amino acid sequence shares 96% identity with the hsfsh-r: not ... | 1997 | 9195473 |
| serodiagnosis of babesia equi infection--a comparison of dot-elisa, complement fixation test and capillary tube agglutination test. | the present study aimed to develop dot-elisa, complement fixation test (cft) and capillary tube agglutination test (cat) for serodiagnosis of babesia equi infection and to compare their sensitivity with each other. for this study, sequential serum samples were collected from four donkeys experimentally infected with b. equi up to 90 days post infection (p.i.). b. equi antigen was prepared from the blood of a donkey showing more than 80% parasitaemia. dot-elisa, cf and ca tests were standardized ... | 1997 | 9195726 |
| a quantitative morphological study of age-related changes in the donkey testis in the period between puberty and senium. | the testis of the donkey was used as a model to study age-related changes in the period between puberty and senium. from the age of 1.5 years to the middle of sexual maturity (5 to 6 years) a number of histophysiological features, all indicative of the spermatogenetic efficiency, increase continuously. then without a longer-lasting plateau of maximal performance these features undergo continuous retrogression. thus, the adult testis is an organ in permanent change. during its progressive period ... | 1997 | 9197920 |
| failure to establish chronic infection of the reproductive tract of the male horse with a south african asinine strain of equine arteritis virus (eav). | eight sexually mature horse stallions were inoculated intranasally with a south african asinine strain of eav, a strain that was isolated from the semen of a donkey carrier. all horses developed fever, with maximum rectal temperatures of 38.9-39.9 degrees c recorded 3-6 d post challenge. six horses showed very mild clinical signs of equine viral arteritis and two were asymptomatic. the virus was recovered from the nasopharynxes of six horses 2-7 d after inoculation, and from buffy-coat samples o ... | 1997 | 9204499 |
| citrulline-argininosuccinate-arginine cycle coupled to ca2+-signaling in rat pancreatic beta-cells. | pancreatic beta-cells possess nitric oxide (no) synthases (noss) which synthesize no and l-citrulline from l-arginine. the present study was designed to explore the mechanism of citrulline and arginine metabolism in beta-cells and its possible coupling to beta-cell functions. the enzymes involved in citrulline-arginine metabolism, argininosuccinate synthetase (ass), argininosuccinate lyase (asl), and nos were expressed in rat islets and insulinoma hit t15 cells. in the presence of stimulatory gl ... | 1997 | 9207208 |
| the additional value of free prostate specific antigen to the battery of age-dependent prostate-specific antigen, prostate-specific antigen density and velocity. | this study describes the value of using the fraction of free prostate-specific antigen as a further marker in the early detection of prostate cancer. this newly introduced marker is compared to the usual battery of age-dependent total prostate-specific antigen, prostate-specific antigen density (microg/l x g tissue) and prostate-specific antigen velocity (microg/l x year). determination of total prostate-specific antigen and free prostate-specific antigen was performed on fresh serum samples obt ... | 1997 | 9228332 |
| topographical and functional investigation of escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis. | penicillin-binding proteins (pbps) are the targets of beta-lactam antibiotics. we have used a systematic five-alanine substitution method (called ass [alanine stretch scanning] mutagenesis) to investigate the functional or structural role of various stretches of amino acids in the pbp1b of escherichia coli. to probe the specific activity of each variant, the antibiotic discs assay was used with strain qcb1 (delta ponb) in the presence of cefaloridine, which totally inhibits the complementing act ... | 1997 | 9244263 |
| fish mapping of the igf2 gene in horse and donkey-detection of homoeology with hsa11. | three genomic subclones derived from a phage clone containing the equine igf2 gene were used to fish map the gene on horse (eca) and donkey (eas) metaphase chromosomes. the gene mapped on eca 12q13 band and is the first locus mapped to this horse chromosome. in donkey the gene mapped very terminal on the long arm of one small submetacentric chromosome that shows almost identical dapi-banding pattern with eca12. this is the first locus mapped in donkey genome. cross species chromosome painting of ... | 1997 | 9250862 |
| [length of gestation of freiberger mares with mule and horse foals]. | in a retrospective study the gestation lengths of two groups, each consisting of 193 "freiberger" mares, were compared. the mares of the first group were bred by a donkey, those of the second group by a stallion of the "freiberger" breed. on average, the gestation length of mares with "freiberger" foals lasted 336.5 days, and that of mares with mule foals 343.1 days. the difference between both groups was significant (p < 0.001). the shortest and longest observed gestation length of mares with " ... | 1997 | 9281060 |
| morphologic evidence for l-citrulline conversion to l-arginine via the argininosuccinate pathway in porcine cerebral perivascular nerves. | results from biochemical and pharmacologic studies suggest that lcitrulline is taken up by cerebral perivascular nerves and is converted to larginine for synthesizing nitric oxide (no). the current study was designed using morphologic techniques to determine whether lcitrulline is taken up into axoplasm of perivascular nerves and to explore the possibility that conversion of lcitrulline to larginine in these nerves is through the argininosuccinate pathway in porcine cerebral arteries. results fr ... | 1997 | 9290586 |
| plasma glucose and cortisol responses to exogenous insulin in fasted donkeys. | susceptibility to equine hyperlipaemia is increased by poor food intake. to assess the contribution of changes in insulin sensitivity, plasma glucose and cortisol responses to an intravenous insulin challenge (0.4 iu kg-1 bodyweight) were compared with those observed after saline administration in six donkeys fasted either overnight or for three days. three days of fasting decreased both the rate of insulin-induced hypoglycemia and the maximal hypoglycemic response. a transitory increase in plas ... | 1997 | 9300546 |
| nadph-diaphorase and cytosolic urea cycle enzymes in the rat spinal cord. | nitric oxide synthase (nos), argininosuccinate synthetase (ass), and argininosuccinate lyase (asl) compose a cyclic pathway to form nitric oxide (no). these enzymes, however, are localized differentially in most regions of the brain. to find out whether nos, ass, and asl are colocalized in neurons of the spinal cord, we examined the distribution of these enzymes by using a double-labeling procedure combining fluorescent immunohistochemistry with an assay for reduced nicotinamide adenine dinucleo ... | 1997 | 9302108 |
| characterization and phylogenetic significance of rhinoceros luteinizing hormone beta (lhbeta) subunit messenger rna structure, complementary dna sequence and gene copy number. | the luteinizing hormone (lh) beta subunit gene is expressed in the pituitary glands of all mammals, whereas the closely related chorionic gonadotropin (cg) beta subunit genes have been identified only in primates and equids, and are expressed in placenta. in the case of horses, there is a single-copy equine (e) luteinizing hormone/chorionic gonadotropin hormone beta subunit gene (elh/cgbeta) that (1) is expressed in both pituitary gland and placenta, (2) encodes a characteristic carboxyl termina ... | 1997 | 9305757 |
| reference ranges and the influence of age and sex on haematological values of the endangered catalonian donkey. | | 1997 | 9308403 |
| central overexpression of the trh precursor gene induces hypertension in rats: antisense reversal. | extrahypothalamic trh participates in cardiovascular regulation and spontaneous hypertension of the rat. to investigate whether an increase in central trh activity produces hypertension we studied the effect of the pretrh overproduction induced by i.c.v. transfection with a naked eukaryotic expression plasmid vector which encodes pretrh (pcmv-trh). northern blot analysis and rt-pcr showed that pcmv-trh was transcribed in vitro and in vivo. at 24, 48, and 72 hours, pcmv-trh (100 microg) in a sign ... | 1997 | 9323019 |
| cosegregation of open-angle glaucoma and the nail-patella syndrome. | to evaluate two families ascertained only for the presence of glaucoma in which both nail-patella syndrome and glaucoma occur in several generations and to determine whether the two diseases are genetically related. | 1997 | 9323941 |
| clinical features of blister beetle poisoning in equids: 70 cases (1983-1996). | to document clinical signs and gross pathologic changes associated with naturally acquired cantharidiasis (blister beetle poisoning) in equids. | 1997 | 9343547 |
| the recognition of methylated dna by the gtp-dependent restriction endonuclease mcrbc resides in the n-terminal domain of mcrb. | mcrbc is a gtp-dependent restriction endonuclease of e. coli k12, selectively directed against dna containing modified cytosine residues. mcrb, one of its components, is responsible for the binding and, together with mcrc, for the cleavage of dnas containing two 5'-pu(m)c sites separated by 40-80 base pairs. gel retardation assays with wild-type and mutant mcrb reveal that (i) single 5'-pu(m)c sites in dna can be sufficient to elicite binding by mcrb. binding to such substrates is, however, weak ... | 1997 | 9348106 |
| effect of the south african asinine-94 strain of equine arteritis virus (eav) in pregnant donkey mares and duration of maternal immunity in foals. | clinical, virological and serological responses were investigated in five pregnant donkey mares after experimental exposure to the south african asinine-94 strain of equine arteritis virus (eav), and the duration of maternal immunity to eav was studied in their foals. in four intranasally inoculated mares, fever with maximum rectal temperatures of 39.1-40.7 degrees c was recorded 2-11 d after challenge. all the inoculated mares developed mild depression, and a serous ocular and nasal discharge; ... | 1997 | 9352564 |
| orthotopic liver transplantation for adult-onset type ii citrullinaemia. | citrullinaemia is a rare autosomal recessive disorder due to a deficiency of argininosuccinate synthetase (ass). while the clinical course of the adult onset form (type ii) is unpredictable, many patients undergo sudden deterioration with progressive cerebral oedema. two patients with type ii citrullinaemia were referred for liver transplantation. one patient was successfully transplanted, and his plasma citrulline level decreased from 300 to 69 mumol/l (normal range 20-60 mumol/l); fisher's rat ... | 1997 | 9361940 |
| alteration of anionic sites in renal glomerular basement membrane of pigs. | ultrastructural alteration of anionic sites (ass) in the glomerular basement membrane (gbm) was studied in 10 cases of swine mesangial proliferative glomerulonephritis using a cationic ultrastructural tracer, 0.5% polyethyleneimine (m.w. = 1,800). glomerular ass were seen as discrete electron-dense particles in the gbm, mesangial matrix and epithelial cell surfaces by electron microscopy. in the lamina rara externa (lre) of the normal gbm, ass were distributed regularly in a single layer. in tho ... | 1997 | 9362031 |
| l-citrulline conversion to l-arginine in sphenopalatine ganglia and cerebral perivascular nerves in the pig. | the presence of nitric oxide synthase (nos), argininosuccinate synthetase (ass), and argininosuccinate lyase (asl) and their coexistence with nadph-diaphorase (nadphd), a marker for nos, in the porcine sphenopalatine ganglia (spg), pial veins, and the anterior cerebral arteries was examined using immunohistochemical and histochemical staining techniques. nos-immunoreactive (i), ass-i, and asl-i fibers were found in pial veins and the anterior cerebral arteries. nos, ass, and asl immunoreactiviti ... | 1997 | 9374753 |
| interspecific and extraspecific pregnancies in equids: anything goes. | equids possess the unusual ability to interbreed freely among the phenotypically and karyotypically diverse member species of the genus to produce viable, but usually infertile, offspring. the mule (female horse x male donkey) was humanity's first successful attempt at genetic engineering and its clear expression of both parental phenotypes has contributed much to our understanding of genetic inheritance over the centuries. even more surprising, mares and donkeys have been shown to be capable of ... | 1997 | 9378914 |
| "the law is a ass--a idiot". | | 1997 | 9385815 |
| [the research of radioimmunoassay using double abs for bradykinin]. | bradykinin (bk) is a potent vasodilative substance, and plays great physiological and pathological roles in animals and human beings. to measure the quantity of bk, the radioimmunoassay (ria) has been devised, but the traditional ria method has certain defects, such as presence of numerows interfering factors and errors and time consuming. now, we produce anti-bk serum in rabbits by using bk-ovalbumin conjugate as an immunogen, and the 125i labeled tyr8-bk by using a modified chloramine-t method ... | 1996 | 9388945 |
| regulation of argininosuccinate synthetase mrna level in rat foetal hepatocytes. | expression of the hepatic gene for argininosuccinate synthase (ass), one of the key enzymes of the urea cycle, was analysed during the perinatal period in the rat. to this end, the amount of specific mrna was measured in the liver at various stages of development and in cultured foetal hepatocytes maintained in different hormonal conditions. the ass mrna was first detected in 15.5-day foetuses and its level increased concomitantly with a rise in the enzyme activity, suggesting that the appearanc ... | 1997 | 9395312 |
| making an ass of the law. | | 1997 | 9401388 |
| evidence that the alpha-subunit influences the specificity of receptor binding of the equine gonadotrophins. | horse lh/chorionic gonadotrophin (elh/cg) exhibits, in addition to its normal lh activity, a high fsh activity in all other species tested. donkey lh/cg (dklh/cg) also exhibits fsh activity in other species, but about ten times less than the horse hormone. in order to understand the molecular basis of these dual gonadotrophic activities of elh/cg and dklh/cg better, we expressed, in cos-7 cells, hybrids between horse and donkey subunits, between horse or donkey alpha-subunit and human cg beta (h ... | 1997 | 9415058 |
| differential effects of somatostatin analogues on proliferation of murine colonic cancer cells in vitro. | the effects of somatostatin analogues octreotide (sms 201-995), ass-51 and ass-52 on [3h]-thymidine incorporation into dna of the murine colon 38 cancer cells in vitro were investigated. it was found that sms 201-995 and ass-51 inhibited the tritiated thymidine incorporation in a dose-dependent manner. in contrast, analogue ass-52 in spite of a very similar structure to ass-51, which differed from the latter only by one ch2oh group, was devoid of remarkable antiproliferative activity. these resu ... | 1997 | 9418016 |
| carbon dioxide laser as a surgical instrument for sarcoid therapy--a retrospective study on 60 cases. | the objective of this retrospective clinical study was to evaluate the carbon dioxide laser in the treatment of single and multiple sarcoids in 60 animals (44 horses, 13 donkeys, 2 mules, and 1 pony). only animals that had been operated on 6 mo or more ago were included. recurrence, new manifestation rate, and cosmetic outcome were determined. recurrence was observed in 23 (38%) individuals. animals with new sarcoid manifestation with or without recurrence of a sarcoid were observed in 35 cases ... | 1997 | 9426943 |
| genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. | a reverse transcription-polymerase chain reaction (rt-pcr) was developed for the genus-specific detection of alphaviruses. based on the available published sequences, degenerate primers were designed to ensure hybridization to a conserved region within the nonstructural protein 1 gene of all alphavirus species. the expected 434-basepair (bp) cdna fragment was amplified from all 27 alphavirus species by using rna extracted from 200 microl of infected cell culture supernatant. in addition, eight s ... | 1997 | 9430533 |
| an epizootic of besnoitiosis in goats in fars province of iran. | over 100,000 goats were grazing freely in isolated herds of 100 to 600 goats in a mountainous area of more than 2000 km2 to the north-east of darab city in fars province in southern iran. twenty to fifty percent of the goats in this area were infected with besnoitiosis. sheep, cattle and donkeys grazing in the same area showed no clinical manifestations of the infection. the diagnosis of the infection in the clinically suspected animals was confirmed by histological studies on skin biopsies from ... | 1997 | 9444079 |
| [prospective study on the prevention of heterotopic ossification after total hip replacement. non-steroidal anti-inflammatory agents versus radiation therapy]. | two prospective trials were undertaken to assess the comparative efficacy of early postoperative irradiation with different radiation doses versus the postoperative use of nonsteroidal antiinflammatory drugs (nsaid) for prevention of heterotopic ossification (ho) following prothetic total hip replacement (thp). | 1997 | 9454352 |
| elucidation of the mechanism enhancing the avidity of lectin with oligosaccharides on the solid phase surface. | the mechanism underlying molecular recognition of lectins was elucidated by a novel solid phase binding assay system based on surface plasmon resonance. when the apparent affinities of interactions between chitooligosaccharides and wheat germ agglutinin were compared between lectin-immobilized and oligosaccharide-immobilized assay systems, the affinity constants (ka) calculated for the former system were in good agreement with the previously reported values measured in solution. on the other han ... | 1997 | 9455921 |
| distribution and co-localization of nitric oxide synthase and argininosuccinate synthetase in the cat hypothalamus. | argininosuccinate synthetase (ass) and nitric oxide synthase (nos) comprise part of the cyclic metabolic pathway to produce nitric oxide (no). ass is one of the arginine synthesis enzymes which synthesizes argininosuccinate from aspartate and citrulline, and nos forms no and citrulline from arginine. this study examines the localization of ass and nos in the cat hypothalamus using nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) histochemistry and immunohistochemistry against ass ... | 1997 | 9477156 |
| [pathophysiology and therapeutic concepts in coronary restenosis]. | demonstration of a reduced restenosis rate after stent implantation (benestent, stress) has initiated rapid increase in stent implantation rates with widening indications. at present, the majority of stents are implanted in "none-benestent/stress-lesions" with the consequence of a higher restenosis rate as previously expected. stent restenosis has therefore become a relevant problem in interventional cardiology. in contrast to balloon angioplasty, where acute and subacute recoil represents the m ... | 1997 | 9483438 |
| screening for bovine papillomavirus in peripheral blood cells of donkeys with and without sarcoids. | papillomaviral dna has been identified in peripheral blood cells of both cattle and humans with and without associated disease and it has been suggested that such cells may act as sites of viral latency. in order to investigate the possibility of latent papillomaviral infection in the aetiopathogenesis of the equine sarcoid, peripheral blood derived dna samples from 20 healthy and 34 sarcoid-affected donkeys were subject to polymerase chain reaction (pcr) using papillomaviral specific primers. a ... | 1997 | 9491459 |
| babesiosis in china. | the importance of babesiosis in livestock in china is discussed and mainly focused on bovine and equine babesiosis. babesiosis is still one of the most important diseases affecting livestock and has caused great economic loss. nine species of babesia have been recognized in livestock: b. bigemina, b. bovis, b. major, b. motasi, b. ovis, b. perroncitoi, b. trautmanni, b. equi (theileria equi), b. caballi. the distribution of babesia follows the distribution of the tick vectors. the main vectors o ... | 1997 | 9512738 |
| [prospective randomized pilot study of ambulatory prevention of thromboembolism. 2 times 500 mg aspirin (ass) vs. clivarin 1750 (nmh)]. | from march 1994 to march 1996, 287 patients, presenting with lower extremity injuries, who required immobilizing bandages or casts, where included in a prospective, randomized study. these patients received either low-molecular heparin or acetylsalicylic acid for thrombosis prophylaxis. in all patients a clinical examination and a colour-coded duplex sonography were performed after removal of the cast for detection of lower extremity venous thrombosis. a plebography was performed when thrombosis ... | 1998 | 9522671 |
| expression of epidermal growth factor and its receptor in equine placental tissues. | northern blot and in situ hybridization techniques have demonstrated a marked increase in mrna encoding epidermal growth factor (egf) in the endometrium of mares, coincident with the start of interdigitation between the allantochorion and endometrium during placentation. in the present study, the unusually high egf expression in the epithelium of the endometrial glands was shown to be maintained until at least day 250 of gestation (term = 320-340 days) in mares carrying normal horse conceptuses. ... | 1998 | 9538329 |
| clinical biochemical parameters of the endangered catalonian donkey breed: normal values and the influence of sex, age, and management practices effect. | twelve clinical biochemical parameters were determined in 97 animals of all age groups and both sexes of the endangered catalonian donkey breed. significant sex differences were observed for phospholipid concentration (p<0.01). evaluating the effect of management practices on the various parameters showed significant differences for total bilirubin (p<0.001) and creatinine (p<0.05) concentrations and gamma-glutamyltransferase (p<0.05) activity. moreover, it was observed that inorganic phosphorus ... | 1998 | 9557798 |
| in vitro propagation of theileria annulata infected schizonts in different media supplemented with heterologous sera. | efficacy of medium rpmi-1640 (supplied by gibco usa, centron and hi-media) supplemented with horse, donkey, sheep and goat sera was evaluated for in vitro propagation of theileria annulata (hisar) infected bovine mononuclear cells. the results were compared with the growth rate in rpmi-1640 supplemented with foetal bovine serum (gibco). rpmi-1640 (gibco) proved to be the best medium for in vitro cultivation of the parasite infected cells. foetal bovine serum could be easily, safely and reliably ... | 1997 | 9567745 |