| regeneration of skeletal muscle in two teleost fish: sparus aurata and brachydanio rerio. | regeneration of skeletal muscle was studied in the sea bream sparus aurata, in which extensive post-larval muscle hyperplasia contributes to its large adult size, and in the zebrafish brachydanio rerio, which shows little post-larval hyperplasia and reaches only a small adult size. small mechanical lesions of body wall muscle were made under general anaesthesia, and the progress of subsequent regeneration was assessed at various intervals by histology and electron microscopy (for general morphol ... | 1997 | 9211834 |
| first events in lipid absorption during post-embryonic development of the anterior intestine in gilt-head sea bream | structural development and lipid absorption in anterior intestine of gilt-head sea bream sparus aurata, were studied by light and electron microscopy during three stages of post-embryonic development: (1) the endotrophic period from hatching day (day 0) to mouth opening day (day 3); (2) the endo-exotrophic period from days 3 to 15; (3) the exotrophic period after day 15. during the 2 days following hatching, there was no trace of lipids in intestinal epithelia. before mouth opening day, the firs ... | 1997 | 9236098 |
| structural c3 diversity in fish: characterization of five forms of c3 in the diploid fish sparus aurata. | in virtually all species examined to date, the functionally active third component of complement (c3) is encoded by a single gene. we have recently demonstrated, however, that trout possess three structurally and functionally distinct active c3 that represent the products of at least two different c3 genes. in the present study, we provide evidence that multiple forms of functional c3 occur not only in the rainbow trout (salmo gairdneri), a quasi-tetraploid old teleost fish, but also in the dipl ... | 1997 | 9058817 |
| adrenergic stimulation of sea bream (sparus aurata) red blood cells in normoxia and anoxia: effects on metabolism and on the oxygen affinity of haemoglobin | the metabolic response of sea bream (sparus aurata) red blood cells to adrenergic stimulation was determined in normoxia and anoxia. in the presence of oxygen, red blood cells swelled and then recovered their resting volume. continuous monitoring of oxygen uptake displayed the kinetics of the increase in the oxygen affinity of haemoglobin. cell volume recovery correlated with an activation of atp consumption, and the energy equilibrium was restored by increasing the rates of respiration and glyc ... | 1997 | 9318750 |
| ultrastructure and cytochemistry study of eimeria sparis (protozoa:apicomplexa) stages from the intestine of gilthead sea bream sparus aurata l. (pisces:teleostei). | the ultrastructure and cytochemistry of merogonial, gamogonial, and early sporogonial stages of eimeria sparis in the intestine of sparus aurata were studied. mature stages showed the typical pellicle, which was lost in some transitional stages. an apparent unit membrane was seen in some immature stages. a parasitophorous vacuole, sometimes with membrane vesicular invaginations, was usually observed. we propose that the stages located over the epithelium and the so-called epicellular stages be t ... | 1997 | 9039694 |
| ultrastructure and cytochemistry study of goussia sparis (protozoa: apicomplexa) stages from the intestine of the gilthead sea bream sparus aurata l. (pisces: teleostei). | the ultrastructure and cytochemistry of merogonial and gamogonial stages and early unsporulated oocysts of goussia sparis in the intestine of sparus aurata were studied. the typical pellicle was observed in some stages. the different stages might appear in intraepithelial or supraepithelial positions, but they were always intracellular. first steps of two apparently different endomerogonies were observed in intra- and supraepithelial positions, respectively. an apparent ectomerogony also occurre ... | 1997 | 9000229 |
| growth hormone as a function of age and dietary protein: energy ratio in a marine teleost, the gilthead sea bream (sparus aurata). | we examined in a factorial design the effect of dietary protein (45%, 52% and 60%) and lipids (8%, 12%, 17%) on growth performance and circulating growth hormone (gh) levels of fingerling sea bream (5-month-old) fed to satiation with self-feeders. daily weight gain (2.6-2.9%) and feed gain ratio (1.1-1.3) of fish fed high protein-low lipid diets were comparable to those found in fast growing strains of rainbow trout. however, increasing hyperphagia in association with the decrease of daily weigh ... | 1996 | 8971555 |
| age-related changes in 2-[(125)i]-iodomelatonin binding sites in the brain of sea breams (sparus aurata, l.). | the pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). the target sites for melatonin are poorly known in fish, especially marine species. a radioligand study was undertaken using the gilthead sea bream (sparus aurata) maintained under natural temperature and photoperiod (at 28°n latitude). this species exhibits the property of changing sex during growth. brains ... | 1996 | 24194300 |
| early organization of the pituitary gland in sparus aurata l. (teleostei). an ultrastructural study. | the cell organization of the pituitary gland and the relationship between neurohypophysis and adenohypophysis in early developmental stages of the gilthead sea bream, sparus aurata, were studied by electron microscopy. in newly hatched larvae, the pituitary gland was embedded in the ventral floor of the diencephalon and separated from the hypothalamus by a continuous basal lamina. elongated mesenchymal cells next to the ventral surface were observed. at this stage, there was no neurohypophysis a ... | 1996 | 8729962 |
| somatotropin release-inhibiting factor and galanin innervation in the hypothalamus and pituitary of seabream (sparus aurata). | the distribution of galanin (gal) and somatotropin-release-inhibiting-factor (srif) immunoreactivity in the hypothalamus and pituitary of the sea bream (sparus aurata) was studied by immunocytochemistry. an extensive system of neurons immunoreactive with antisera to the two peptides was identified throughout the brain with staining particularly in the hypothalamus. in the hypothalamus, gal immunoreactive perikarya were detected principally in the nucleus preopticus and nucleus tuberis. major ner ... | 1996 | 8729936 |
| effect of caulerpenyne, a toxin extracted from caulerpa taxifolia on mechanisms regulating intracellular ph in sea urchin eggs and sea bream hepatocytes. | the proliferation of the green marine alga caulerpa taxifolia in the mediterranean led us to investigate the toxic effects on marine organisms of caulerpenyne (cyn), the major secondary metabolite synthesized by the alga. this study was performed on sea urchin eggs (paracentrotus lividus) and isolated hepatocytes from the sea bream (sparus aurata), in which accumulation of the toxins by metabolic processes may be of significance. cyn provoked an acidification of seawater containing both unfertil ... | 1996 | 9110246 |
| hematological recovery in sparus aurata after bleeding. a time course study. | to determine the hematological recovery after blood loss in gilthead sea bream juveniles, an experimental anemia was induced by caudal blood extraction. seventy fish were used for experimental hemorrhage and another 35 were used as controls. blood samples were taken after 0.5, 1, 2, 4, 8, 16 and 32 days in both control and anemic groups. after bleeding, besides a reduction in the direct hematic constants, a peak in the mean cell volume value, together with a decrease in mean cell hemoglobin conc ... | 1995 | 8907437 |
| amino acid composition and endocrine control of vitelline envelope proteins in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata). | the vitelline envelopes of european sea bass and gilthead sea bream are both composed of mainly four proteins with the molecular masses of 90, 52, 48, 45 kda and 75, 50, 48, 44 kda, respectively. each protein has an amino acid composition that is characterized by a high content of proline and glutamic acid and a low content of cysteine, similar to the whole vitelline envelope of both species. the amino acid composition suggests that each protein is distinct but related to the other vitelline env ... | 1995 | 8588933 |
| somatolactin, a novel pituitary protein: isolation and characterization from sparus aurata. | the complete amino acid sequence of somatolactin, a new pituitary protein belonging to the growth hormone/prolactin family, from the gilthead sea bream sparus aurata has been determined. somatolactin was isolated from the pituitary by alkaline extraction, gel filtration on a sephadex g-100 column, and reversed-phase high-performance liquid chromatography (rphplc) on a tsk gel ods-120t column. the purified protein was confirmed to be somatolactin by immunoblotting using chum salmon somatolactin a ... | 1995 | 7773329 |
| mitochondrial dna variation in an experimental stock of gilthead sea bream (sparus aurata). | we present a restriction site map for the mitochondrial dna of the gilthead sea bream and the consensus sequence for a region of the mitochondrial cytochrome b gene. restriction site and nucleotide sequence analysis was used to obtain individual mitotype profiles in a broodstock used for genetic studies. eight mitotypes were detected among 33 animals assayed. in combination with hypervariable nuclear markers, this variation can be used to identify the parents of any offspring in mass matings and ... | 1995 | 7773328 |
| ration size and protein intake affect circulating growth hormone concentration, hepatic growth hormone binding and plasma insulin-like growth factor-i immunoreactivity in a marine teleost, the gilthead sea bream (sparus aurata). | the nutritional regulation of the growth hormone liver axis has been studied in gilthead sea bream (sparus aurata). in a first study, fingerling fish were fed three experimental diets with varying proportions of protein (34, 45 and 55%). a 60% decrease in plasma growth hormone concentration was observed with the increase of specific growth rates and dietary protein levels. an opposite response was observed in hepatic growth hormone-binding sites and plasma insulin-like growth factor-i immunoreac ... | 1995 | 7876930 |
| perinotochordal connective sheet of gilthead sea bream larvae (sparus aurata, l.) affected by axial malformations: an histochemical and immunocytochemical study. | spinal malformations in adult teleosts occur under natural conditions and, more frequently, in culture exploitations. skeletal deformities are linked with dysfunctions in collagen metabolism. we studied axial deviations appearing in early larval stages of cultured sea bream (sparus aurata, l.). | 1994 | 7992891 |
| isolation of growth hormone and in vitro translation of mrna isolated from pituitaries of the gilthead sea bream sparus aurata. | growth hormone (gh) polypeptide was purified from pituitary glands of the gilthead sea bream (sparus aurata) by a two-step procedure involving gel filtration on sephadex g-100 and reverse-phase high-performance liquid chromatography (rphplc). at each stage of purification, fractions were monitored by sds-polyacrylamide gel electrophoresis (sds-page) and by immunoblotting using anti-bonito gh antiserum. the molecular weight of the sea bream gh was estimated by sds-page to be 21 kda when electroph ... | 1994 | 7821767 |
| skeletal deformities of the gilthead sea bream (sparus aurata, l.): study of the subcommissural organ (sco) and reissner's fiber (rf). | a high incidence of lordotic curvatures has been detected in commercial cultures of sparus aurata. we have studied juvenile and adult lordotic specimens to elucidate whether the subcommissural organ and its secretion, the reissner's fiber, play any role in the development of this syndrome. animals were x-radiographed and then the brain and spinal cord dissected out and processed for light microscopy. adult lordotic fishes had a well developed swim-bladder whereas juvenile did not. the central ca ... | 1994 | 8085662 |
| seasonal changes in circulating growth hormone (gh), hepatic gh-binding and plasma insulin-like growth factor-i immunoreactivity in a marine fish, gilthead sea bream,sparus aurata. | we have studied the seasonal relationship between growth and circulating growth hormone (gh), hepatic gh-binding and plasma insulin-like growth factor-i immunoreactivity in gilthead sea bream,sparus aurata. the seasonal increase in plasma gh levels preceded by several weeks the summer increase in growth rates. in contrast, a marked increase in hepatic gh-binding with a high degree of endogenous gh occupancy was found during the period of maximum growth which suggests an enhanced sensitivity of l ... | 1994 | 24198190 |
| in vivo metabolism of [1-14c]linolenic acid (18:3(n-3)) and [1-14c]eicosapentaenoic acid (20:5(n-3)) in a marine fish: time-course of the desaturation/elongation pathway. | the metabolism (via the desaturation/elongation pathways) of [1-14c]18:3(n-3) and [1-14c]20:5(n-3) in a marine fish, gilthead sea bream (sparus aurata l.), were investigated over 8 days to determine the time-courses for the production of delta 6 and delta 5-desaturase products and 22:6(n-3). fish were starved for 1 week prior to, and during, the period of the experiment. the recovery of radioactivity from [1-14c]20:5(n-3) in tissue lipids exceeded that of [1-14c]18:3(n-3) at all time points. the ... | 1994 | 7908832 |
| developmental regulation of growth hormone binding in the gilthead sea bream, sparus aurata. | the occurrence of growth hormone (gh) binding was investigated in the gilthead sea bream larva, sparus aurata, using recombinant trout ghii (rtgh) as a tracer. gh binding was detected in all the specimens examined (5, 17, 27 and 40 days after hatching). at 40 days after hatching, we observed a concentration of gh binding in the head region. specific binding was dependent on protein membrane concentration and scatchard plots showed a single class of high affinity and low capacity binding sites. s ... | 1994 | 8193580 |
| incorporation and metabolism of(14)c-labelled polyunsaturated fatty acids in wild-caught juveniles of golden grey mullet,liza aurata, in vivo. | the incorporation, and the capacity for desaturation and elongation in vivo, of intraperitoneally-injected,(14)c-labelled n-3 and n-6 c18 and c20 polyunsaturated fatty acids (pufa) were investigated in juvenile golden grey mullet,liza aurata. the results indicate that juvenile mullet have only limited ability to convert c18 polyunsaturated fatty acids to c20 and c22 highly unsaturated fatty acids (hufa)in vivo. this suggests that juvenile golden grey mullet require the provision of preformed c20 ... | 1993 | 24202691 |
| a histochemical and immunohistochemical study of digestive enzymes and hormones during the larval development of the sea bream, sparus aurata l. | the distribution of different hydrolytic enzymes and the localization of the hormones which regulate glucose metabolism during development of the digestive tract of the sea bream, sparus aurata l., were studied. the yolk sac contains trypsin, glucose-6-phosphatase, atpases and acid and alkaline phosphatase activities. positive insulin, glucagon and somatostatin cells were observed in the pancreas and in the lumen of the intestinal tract during endogenous feeding. from hatching until 3 days later ... | 1993 | 7689548 |
| influence of environmental salinity on prolactin and corticotropic cells in the gilthead sea bream (sparus aurata l.). | the influence of the environmental salinity on the prolactin (prl) and corticotropic (acth) cells of the rostral pars distalis of the adenohypophysis of the gilthead sea bream (sparus aurata) adapted to sea water (sw, 980 mosm/kg) and brackish water (bw, 200 mosm/kg) has been studied by immunocytochemical, morphometric, and electron-microscopic techniques. prolactin (prl) cells of fish adapted to bw occupied a greater hypophysial volume (about 24% of the total hypophysial volume in bw, 10% in sw ... | 1993 | 8391498 |
| incorporation and metabolism of (14)c-labelled polyunsaturated fatty acids in juvenile gilthead sea bream sparus aurata l. in vivo. | the incorporation, and the capacity for desaturation and elongation in vivo, of intraperitoneally-injected, (14)c-labelled n-3 and n-6 c18 and c20 pufas were investigated in juvenile gilthead sea bream, sparus aurata. the results indicate that juvenile gilthead sea bream have only limited ability to convert ch pufas to c20 and c22 hufas in vivo. the data are consistent with the results from nutritional studies on larvae, postlarvae and fingerlings that have shown that gilthead sea bream require ... | 1993 | 24214444 |
| developmental expression of the growth hormone gene in the gilthead sea bream sparus aurata. | expression of growth hormone (gh) gene during early stages of larval development of the teleost sparus aurata was determined by northern blot analysis. poly(a+) rna was prepared from a pool of larvae collected on different days after hatching. when hybridized to sparus aurata gh cdna, gh specific mrna was first seen on day 6 post-hatching. in contrast, the levels of beta-actin mrna, which was used to normalize for rna amounts, were already high on the day of hatching. our results suggest that ex ... | 1992 | 1359982 |
| somatostatin 14- and somatostatin 25-like peptides in pancreatic endocrine cells of sparus aurata (teleost): a light and electron microscopic immunocytochemical study. | an immunocytochemical investigation demonstrates the presence of somatostatin (sst) 14- and salmon somatostatin (ssst) 25-like peptides in two populations of somatostatin (d) cells in the islets of gilthead sea bream (sparus aurata). both cell types were identified by their differing immunoreactivities to the somatostatin antisera used. d1 cells in the islet periphery between glucagon cells showed ssst 25-like immunoreactivity and contained large moderate to low electron-dense granules. d2 cells ... | 1992 | 1383077 |
| light and electron microscopic immunocytochemical demonstration of the coexistence of somatostatin 14- and somatostatin 25-like peptides in endocrine cells of the stomach of sparus aurata (teleost). | an immunofluorescence double-staining method colocalized somatostatin 14 (sst 14)- and somatostatin 25 (sst 25)-like immunoreactivities in endocrine cells located in the depth of gastric folds and upper part of the stomach glands of sparus aurata (gilthead sea bream). an immunogold method identified somatostatin-like peptides in the secretory granules of the previously described type iv endocrine cells. appropriate preabsorption controls demonstrated two different granule populations with somato ... | 1991 | 1685719 |
| effect of broodstock diets on total lipids and fatty acid composition of larvae of gilthead sea bream (sparus aurata l.) during yolksac stage. | total lipid content and total lipid fatty acid compositions were studied in larvae at the yolksac stage of gilthead sea bream from two different broodstocks. the two broodstock diets had the same total lipid content but were different with respect to their fatty acid levels.there were differences in the fatty acid composition of total lipids in the two groups of larvae, reflecting the influence of fatty acid levels in the diets fed to the broodstock. the main fatty acids in both larvae lots duri ... | 1990 | 24221943 |
| effect of broodstock diets on lipid classes and their fatty acid composition in eggs of gilthead sea bream (sparus aurata l.). | total lipid content, total lipid fatty acids, lipid classes and their fatty acid compositions were studied in eggs of gilthead sea bream from two different broodstocks.the two broodstock diets used had the same total lipid content, but differed with respect to their fatty acid levels. the lipid class composition of eggs from different origins was not significantly different. the predominant polar lipids were phosphatidylcholine, phosphatidylethanolamine and sphingomyelin, and the main neutral li ... | 1990 | 24221942 |
| immunocytochemical and ultrastructural characterization of the cell types in the adenohypophysis of sparus aurata l. (teleost). | the structure and immunocytochemistry of the adenohypophysis of sexually mature male specimens of sparus aurata (gilthead sea bream) were studied. the adenohypophysis was composed of rostral pars distalis (rpd), proximal pars distalis (ppd), and pars intermedia (pi). in the rpd the prolactin cells were organized into follicles which occupied a very reduced area as corresponds to that in saltwater fishes; the corticotropic cells were surrounding the pars nervosa branches and the prolactin follicl ... | 1988 | 2848744 |
| an immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, sparus auratus l. | endocrine cells in the gut of sparus auratus l. (gilt-head sea bream) have been demonstrated by immunocytochemical and electron microscopic techniques. cells showing somatostatin and gastrin-like immunoreactivity were found in the depth of the gastric folds and in the upper part of the stomach glands while substance p immunoreactive cells were present between the upper epithelial cells of the gastric folds. cells showing gastrin, substance p, pancreatic polypeptide, cholecystokinin, and met-enke ... | 1986 | 2433180 |
| differentiation of sparidae species by dna sequence analysis, pcr-sscp and ief of sarcoplasmic proteins. | the increasing global trade of fishery and aquaculture products makes it necessary to develop methods for species identification in case of fish fillets or other highly processed seafood with external morphological characteristics (e.g. gills, fins) of the original fish being removed. species identification methods based on dna- or protein-analysis, like dna sequencing of pcr products, pcr-sscp (polymerase chain reaction - single strand conformation polymorphism), and isoelectric focusing (ief) ... | 2013 | 23265470 |
| melatonin release from the pineals of two sparids. sparus aurata and acanthopagrus bifasciatus. | | 1999 | 10810502 |
| molecular cloning and characterization of gilthead sea bream (sparus aurata) growth hormone receptor (ghr). assessment of alternative splicing. | the full-length growth hormone receptor (ghr) of gilthead sea bream (sparus aurata) was cloned and sequenced by rt-pcr and rapid amplification of 5'and 3'ends. the open reading frame codes for a mature 609 amino acid protein with a hydrophobic transmembrane region and all the characteristic motifs of ghrs. sequence analysis revealed a 96 and 76% of amino acid identity with black sea bream (acanthopagrus schlegeli) and turbot (scophthalmus maximus) ghrs, respectively, but this amino acid identity ... | 2003 | 12941635 |
| in vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses. | the potential effect of the intraperitoneal or dietary administration of propolis, a honeybee product, on gilthead seabream (sparus aurata l.) innate immune responses was evaluated. fish were intraperitoneally injected with 5mg of water (wep), ethanol (eep) or both (wep + eep) extracts of propolis and sampled after 1, 3, 5 and 10 days. when administered in the diet, propolis was dissolved in ethanol and added to a pellet diet at a concentration of 0, 0.1 or 10 g kg(-1) diet, the fish being sampl ... | 2005 | 15450970 |
| new tetrachromic vof stain (type iii-g.s) for normal and pathological fish tissues. | a new vof type iii-g.s stain was applied to histological sections of different organs and tissues of healthy and pathological larvae, juvenile and adult fish species (solea senegalensis; sparus aurata; diplodus sargo; pagrus auriga; argyrosomus regius and halobatrachus didactylus). in comparison to the original gutiérrez vof stain, more acid dyes of contrasting colours and polychromatic/metachromatic properties were incorporated as essential constituents of the tetrachromic vof stain. this facil ... | 2008 | 15967749 |
| histochemical study of lymphocystis disease in skin of gilthead seabream, sparus aurata l. | a battery of horseradish peroxidase-conjugated lectins (con a, wga and dba), as well as conventional histochemical techniques (pas, saponification, alcian blue ph 0.1, 1, 2.5, chlorhydric hydrolisis, sialidase, bromophenol blue, tioglycollate reduction and ferric-ferricyanide-feiii) were used to study the content and distribution of carbohydrates, proteins and glycoconjugate sugar residues on the skin and on the lymphocystis-infected cells of gilthead seabream, sparus aurata. variable amounts of ... | 1998 | 9476632 |
| assessment of trace metal concentrations in muscle tissue of certain commercially available fish species from kayseri, turkey. | regular consumption of fish has been widely recommended by health authorities. however, it is known that some species accumulate high levels of contaminants including heavy metals (e.g., hg, cd, pb, and as). in this study, cu, pb, co, ni, cr, mn, cd, and fe were determined in the muscle tissue of 11 commercially available fish species (sparus auratus, dicentrarchus labrax, mullus barbatus, belone belone, psetta maxima, epinephelus aeneus, salmothymus, soleidae, pomatomus saltatrix, engraulis enc ... | 2014 | 24633787 |
| organochlorine contamination in some marketable fish in egypt. | ten organochlorine compounds were analyzed in four species of fish (bouri mugil spp., denis sparus auratus, moza boops boops and mousa pegusa lascaris), from four egyptian governorates (south sinai, suez, port-said and demietta). although all fish studied are bottom or near-bottom feeders, remarkable variations between studied species were noticed. the grand total values recorded at the studied governorates were in descending order: bouri>denis>moza>mousa. the highest concentration of pesticides ... | 2004 | 14659941 |
| thynnascaris rhacodes sp. n. (nematoda: ascaridoidea) in fishes from the israeli mediterranean coast. | thynnascaris rhacodes sp. n. infects pelates quadrilineatus, solea vulgaris, boops boops, lithognathus mormyrus, obleida melanura, diplodus vulgaris, d. sargus, and sparus auratus from the eastern mediterranean sea. unique in that the cuticle on the female tail is wrinkled, the ascaridoid is further characterized by possessing 27 to 35 precloacal papillae, a body length of 11 to 85 mm, similar spicules 3 to 6% of body length, a length-ratio of intestinal cecum to ventricular appendage of 1 : 1 t ... | 2003 | 570370 |
| essa1 embryonic stem like cells from gilthead seabream: a new tool to study mesenchymal cell lineage differentiation in fish. | embryonic stem (es) cells are a promising tool for generation of transgenic animals and an ideal experimental model for in vitro studies of embryonic cell development, differentiation and gene manipulation. here we report the development and initial characterization of a pluripotent embryonic stem like cell line, designated as essa1, derived from blastula stage embryos of the gilthead seabream (sparus aurata, l). essa1 cells are cultured in leibovitz's l-15 medium supplemented with 5% fetal bovi ... | 2012 | 22903186 |
| in vitro effect of peas, pisum pisum, and chickpeas, cicer arietinum, on the immune system of gilthead seabream, sparus aurata. | the future for a sustainable aquaculture relies on the formulation of feed including alternatives to fish meal and fish oil that do not impair fish growth and that improve fish health status. grain legumes such as field peas and chickpeas offer good sources of proteins, carbohydrates, fibers, vitamins, and minerals. the effect of peas and chickpeas on the immune system of seabream was assessed in vitro in order to detect any potential immunosuppressing problem. peas was determined to be a better ... | 2012 | 22752638 |
| dietary dehydrated lemon peel improves the immune but not the antioxidant status of gilthead seabream (sparus aurata l.). | lemon (citrus limon) is the third most important species of citrus in the world, while spain is the major producer in europe. numerous beneficial effects of lemon are known, which explains their use in traditional medicine. the paper describes the effect of dietary dehydrated lemon peel (a sub-product of the lemon industry) on the growth, immune and antioxidant status of gilthead seabream (sparus aurata l.) over a period of 30 days. fish fed diets enriched with dehydrated lemon peel (1.5% and 3% ... | 2017 | 28359944 |
| retrograde positive contrast urethrocystography of the fish urogenital system. | the radiological differences between the urinary tract of dicentrarchus labrax, sparus aurata, tinca tinca, and cyprinus carpio are shown. in fresh water teleosts the urinary bladder is sigmoid and a short urethra leads to the urinary pore. genital and anal pores are present. in sparus aurata the urinary bladder has a globoid shape. in dicentrarchus labrax the urinary bladder is smaller and elongate. in both marine teleosts a single urogenital pore is visible. positive contrast was used to surv ... | 2014 | 25180198 |
| alpha2-macroglobulin in the marine fish sparus aurata. | the alpha2-macroglobulin proteinase inhibitors (alpha2ms) are a family of plasma proteins with the unique ability to inhibit a broad spectrum of proteinases, but are also known as binding proteins for many growth factors and cytokines, including growth hormone and members of the transforming growth factor-beta superfamily. a partial cdna (475 amino acids) encoding the c-terminus of alpha2m was cloned from the liver of the marine teleostean fish sparus aurata. the deduced amino acid sequence of t ... | 2005 | 16054852 |
| gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (cyprinus carpio) and gilthead seabream (sparus aurata). | our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. we compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (cyprinus carpio) and gilthead seabream (sparus aurata), known to be relatively tolerant to dietary carbohydrates. after cloning of partial cdna sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (g6pase), fructose biphosphatase (fbpase) and phosphoenolpyruvate carboxykinase ( ... | 2002 | 12401217 |
| molecular cloning, tissue distribution and sequence analysis of complete glucokinase cdnas from gilthead seabream (sparus aurata), rainbow trout (oncorhynchus mykiss) and common carp (cyprinus carpio). | the enzyme glucokinase (gk) (ec 2.7.1.1) plays an important role in the control of glucose homeostasis. qualitative and/or quantitative variations in gk enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (sparus aurata) and rainbow trout (oncorhynchus mykiss) than in common carp (cyprinus carpio). in this study, we report the isolation and characterisation of a full-length cdna coding for gk in these teleosts. amino acid ... | 2000 | 10699491 |
| ultrastructure of nonspecific cytotoxic cells in teleosts. i. effector-target cell binding in a marine and a freshwater species (seabream: sparus aurata l., and carp: cyprinus carpio l.). | fish cytotoxic effectors form a cell population whose ultrastructure and properties of conjugation with target cells have not been completely established. we report the ultrastructure of the nonspecific cytotoxic cells in a seawater teleost (sparus aurata l.) and compare it to a freshwater species (cyprinus carpio l.). | 1994 | 7978369 |
| all-fish gene constructs for growth hormone gene transfer in fish. | in order to develop all-fish expression vectors for microinjection into fertilized fish eggs, we have prepared the following constructs: rainbow trout metallothionein a/b and the gilthead seabream growth hormone cdna (ptmta-gbsghcdna, ptmtb-gsbghcdna), carp β-actin gilthead seabream gh cdna (pcaβ-gsbghcdna). the inducible metallothionein promoters a and b were cloned from rainbow trout, and the constitutive promoter β-actin was isolated from carp.the metallothionein promoters were cloned by usin ... | 1993 | 24202494 |
| transcriptomic characterization of the larval stage in gilthead seabream (sparus aurata) by 454 pyrosequencing. | gilthead seabream (sparus aurata) is a teleost belonging to the family sparidae with a high economical relevance in the mediterranean countries. although genomic tools have been developed in this species in order to investigate its physiology at the molecular level and consequently its culture, genomic information on post-embryonic development is still scarce. in this study, we have investigated the transcriptome of a marine teleost during the larval stage (from hatching to 60 days after hatchin ... | 2012 | 22160372 |
| identification of six novel cc chemokines in gilthead seabream (sparus aurata) implicated in the antiviral immune response. | chemokines are key regulators of migration and consequent activation of migrating leukocytes. cc chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish danio rerio. further studies concerning fish chemokine genes will help elucidate the complexity of this chemokine group which has considerably expanded in some teleosts. in the current work, we have identified six novel cc chemokine genes within ... | 2010 | 20096460 |
| evolution of lipopolysaccharide (lps) recognition and signaling: fish tlr4 does not recognize lps and negatively regulates nf-kappab activation. | it has long been established that lower vertebrates, most notably fish and amphibians, are resistant to the toxic effect of lps. furthermore, the lack of a tlr4 ortholog in some fish species and the lack of the essential costimulatory molecules for lps activation via tlr4 (i.e., myeloid differentiation protein 2 (md-2) and cd14) in all the fish genomes and expressed sequence tag databases available led us to hypothesize that the mechanism of lps recognition in fish may be different from that of ... | 2009 | 19201835 |
| thyroid hormones in growth and development of fish. | the thyroid hormones (ths), thyroxine (t(4)) and triiodothyronine (t(3)) are products of the thyroid gland in all vertebrates. their role in early development and metamorphosis is well established in mammals and amphibians, respectively, and recently several studies in fish have highlighted the importance of ths during flatfish metamorphosis. ths are present in high quantities in fish eggs and are presumably of maternal origin. during embryogenesis the concentration of t(4) and t(3) in the eggs ... | 2001 | 11738632 |
| description and comparative study of physico-chemical parameters of the teleost fish skin mucus. | the study of mucosal surfaces, and in particular the fish skin and its secreted mucus, has been of great interest recently among immunologists. measurement of the viscosity and other physico-chemical parameters (protein concentration, ph, conductivity, redox potential, osmolality and density) of the skin mucus can help to understand its biological functions. we have used five marine species of teleost: gilthead seabream (sparus aurata l.), european sea bass (dicentrarchus labrax l.), shi drum (u ... | 2015 | 26484392 |
| shift in trophic level of mediterranean mariculture species. | the mean trophic level of the farmed fish species in the mediterranean has been increasing. we examined the farming-up hypothesis (i.e., the increase in the production of high-trophic-level species) in the mediterranean by determining the trophic level of the aquafeeds (i.e., what the fish are fed) of 5 species of farmed marine fishes: common dentex (dentex dentex), common pandora (pagellus erythrinus), european seabass (dicentrarchus labrax), gilthead seabream (sparus aurata), and red porgy (pa ... | 2014 | 24628519 |
| ozone disinfection of eggs from gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy, and common dentex dentex dentex. | the risk of fish pathogen transmission via eggs can be reduced by disinfection in ozonated seawater. the aim of this study was to determine the suitable conditions for ozone disinfection of the eggs of gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy pagrus pagrus, and common dentex dentex dentex. the eggs were disinfected with a concentration (c) of 0.5 mg of ozone/l of water at four different exposure times (t = 2, 4, 8, and 16 min). the hatching rate was determined in ... | 2012 | 22838084 |
| the tongue dorsal surface in fish: a comparison among three farmed species. | the oral cavity of vertebrates has a very important role in many functions strictly related to the food processing. two species, sea bass dicentrarchus labrax and seabream sparus aurata, are mainly involved in the aquaculture industry in the mediterranean area; moreover, the white seabream diplodus sargus sargus was recently chosen as the best candidate for the diversification of species of commercial interest. this investigation was carried out, using the standard procedures for light and scann ... | 2017 | 27990675 |
| the bromodomain testis-specific gene (brdt) characterization and expression in gilthead seabream, sparus aurata, and european seabass, dicentrarchus labrax. | multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing reproduction of fish species. brdt gene belongs to the bromodomain-extraterminal domain (bet) family of transcriptional coregulators. in mammals, brdt gene is almost exclusively expressed in testis. furt ... | 2016 | 27349318 |
| importance of prebiotics in aquaculture as immunostimulants. effects on immune system of sparus aurata and dicentrarchus labrax. | infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. these components are represented by prebiotics, probiotics, and plant ext ... | 2016 | 27074444 |
| in vitro effects of metals on isolated head-kidney and blood leucocytes of the teleost fish sparus aurata l. and dicentrarchus labrax l. | the in vitro use of fish leucocytes to test the toxicity of aquatic pollutants, and particularly the immutoxicological effects, could be a valuable alternative to fish bioassays but has received little attention. in this study, head-kidney and peripheral blood leucocytes (hkls and pbls, respectively) from gilthead seabream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) specimens were exposed to cd, mehg (methylmercury), pb or as for 24 h being evaluated the resulting cytotoxi ... | 2016 | 27041665 |
| nodavirus colonizes and replicates in the testis of gilthead seabream and european sea bass modulating its immune and reproductive functions. | viruses are threatening pathogens for fish aquaculture. some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (nnv). in order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. however, whether nnv colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. we have de ... | 2015 | 26691348 |
| high diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species. | animal-associated microbiotas form complex communities, which are suspected to play crucial functions for their host fitness. however, the biodiversity of these communities, including their differences between host species and individuals, has been scarcely studied, especially in case of skin-associated communities. in addition, the intraindividual variability (i.e. between body parts) has never been assessed to date. the objective of this study was to characterize skin bacterial communities of ... | 2015 | 26048284 |
| rapid extraction and reverse phase-liquid chromatographic separation of mercury(ii) and methylmercury in fish samples with inductively coupled plasma mass spectrometric detection applying oxygen addition into plasma. | a simple and sensitive procedure was developed for extraction and speciation of mercury in fish. species separation was accomplished with reverse phase-high performance liquid chromatography (hplc) hyphenated to inductively coupled plasma mass spectrometry (icp-ms). oxygen addition into plasma allowed use of organic-rich mobile phase, achieving species separation in 4 min. mercury species extraction was achieved by microwave exposure for 2 min at mild conditions (60°c, ph 2.0), avoiding necessit ... | 2015 | 25872437 |
| trace metals in tissues of the six most common fish species in the black sea, turkey. | concentrations of mn, fe, co, ni, as, se, cd, ag and pb in scale, skin, muscle, gills, liver and the gonads of gilthead seabream (sparus aurata), european seabass (dicentrarchus labrax), black sea salmon (salmo trutta labrax), horse mackerel (trachurus trachurus), red mullet (mullus barbatus) and whiting (merlangius merlangius euxinus) from the black sea, in turkey, were investigated. elemental analyses were performed with inductively coupled plasma-mass spectrometry after sample preparation by ... | 2015 | 25082436 |
| farming-up coastal fish assemblages through a massive aquaculture escape event. | we investigated the changes on the mean trophic level of fish assemblages across different spatiotemporal scales, before and after a massive escape event occurred off la palma (canary islands), which resulted in the release of 1.5 million fish (mostly dicentrarchus labrax) into the wild. the presence of escaped fish altered significantly the mean trophic level of fish assemblages in shallow coastal waters. this alteration was exacerbated by the massive escape. a nearby marine protected area buff ... | 2014 | 24725509 |
| mercury (hg) and methyl mercury (mehg) concentrations in fish from the coastal lagoon of orbetello, central italy. | total mercury (hg tot) and methyl mercury (mehg) were quantified in several specimens of dicentrarchus labrax and sparus aurata from the east basin of the orbetello lagoon, central italy. the size of each specimen was recorded to estimate body burdens (bbs); =hg tot and mehg were measured in fillets of both species. hg tot and mehg in s. aurata ranged between 0.355-1.58 and 0.341-1.53 μg/g wet weight (ww), respectively; in d. labrax, their ranges were 0.284-2.54 and 0.214-2.35 μg/g ww. approxima ... | 2013 | 23998853 |
| presence of contracaecum spp. in teleosts cultured and fished in sardinia. | this study reports the results of the finding of contracaecum spp. during a survey on endoparasites isolated from cultured and wild fish and also from some cephalopods caught in sardinian waters. contracaecum spp. is a nematode belonging to the anisakidae, and is reported to cause zoonosis in humans. nematodes were detected after visual inspection and enzymatic digestion and then identified by morphologic observation, which was confirmed by pcr. the results show that contracaecum spp. were found ... | 2013 | 23597773 |
| regulation of natural killer enhancing factor (nkef) genes in teleost fish, gilthead seabream and european sea bass. | peroxiredoxins (prx) are a family of antioxidant proteins also involved in inflammation and innate immunity. prx1 and prx2 are also known as natural killer enhancing factor (nkef)-a and nkef-b, respectively, by their ability to prime the mammalian nk-cells activity. in teleost fish, nkef genes have been isolated but their regulation has been scarcely evaluated. we have identified orthologues of the nkef-a and nkef-b genes in the teleost european sea bass (dicentrarchus labrax) which showed const ... | 2013 | 23511025 |
| nodavirus infection induces a great innate cell-mediated cytotoxic activity in resistant, gilthead seabream, and susceptible, european sea bass, teleost fish. | viral nervous necrosis (vnn) virus produces great mortalities in fish having susceptible and reservoir species between the most important marine aquaculture species. cell-mediated cytotoxicity (cmc) is considered, towards the interferon (ifn), the most important mechanism of the immune response to fight against viral infections but it has been very scarcely evaluated. we aimed to evaluate the effects of vnnv infection in the reservoir gilthead seabream (sparus aurata) and susceptible european se ... | 2012 | 22981914 |
| cloning and expression analysis of follicle-stimulating hormone and luteinizing hormone receptor during the reproductive cycle in korean rockfish (sebastes schlegeli). | full-length cdna sequences encoding the receptors for follicle-stimulating hormone (fshr) and luteinizing hormone (lhr) were isolated from ovary of korean rockfish (sebastes schlegeli) using reverse transcription-polymerase chain reaction (pcr) and rapid amplification of cdna ends procedures. the cdna of the krfshr encodes a predicted protein of 703 amino acids that showed the greatest homology with european seabass (dicentrarchus labrax) (78 %) and gilthead seabream (sparus aurata) (73 %). the ... | 2013 | 22843313 |
| differentiation of fresh and frozen/thawed fish, european sea bass (dicentrarchus labrax), gilthead seabream (sparus aurata), cod (gadus morhua) and salmon (salmo salar), using volatile compounds by spme/gc/ms. | a simple method based on solid phase microextraction/gas chromatography/mass spectrometry (spme/gc/ms) was applied for studying the volatile profiles of whole fish samples of european sea bass (dicentrarchus labrax) and gilthead seabream (sparus aurata) and fillets of cod (gadus morhua) and salmon (salmo salar) during frozen storage in order to be able to differentiate a fresh product from one that has been frozen. analysis of volatile compounds was performed on these two product types, fresh an ... | 2012 | 22641336 |
| trace elements and metals in farmed sea bass and gilthead bream from tenerife island, spain. | the aim of this study was to determine the levels of metals (ca, k, na, mg) and trace metals (ni, fe, cu, mn, zn, pb, cd) in two fish species (gilthead bream [sparus aurata] and sea bass [dicentrarchus labrax]) collected from fish farms located along the coast of tenerife island. ca, k, na, mg, fe, cu, zn, and mn were measured by flame atomic absorption spectrometry, whereas pb, cd, and ni were determined using graphite furnace atomic absorption spectrometry. mean fe, cu, mn, and zn contents wer ... | 2011 | 22054198 |
| the influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and dna fragmentation. | during cryopreservation, dilution in the extender media reduces the seminal plasma constituents being cells more vulnerable to oxidative stress. vitamins (c and e) and the amino acids taurine and hypotaurine are powerful antioxidants naturally present in seminal plasma. whether their effect may improve sperm quality and reduce sperm dna damage after cryopreservation in fish sperm still remains unclear. thus, the aim of the present work was to analyse the effect of extender supplementation with s ... | 2011 | 21482049 |
| molecular cloning and characterization of european seabass (dicentrarchus labrax) and gilthead seabream (sparus aurata) complement component c3. | we present the complete c3 cdna sequence of gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) and its molecular characterization with a descriptive analysis of their structural elements. we obtained one sequence for gilthead seabream (gsbc3) which encodes a predicted protein of 1656 amino acids, and two sequences for european seabass (esbc3_1 and esbc3_2) which encode two predicted proteins of 1654 and 1587 amino acids respectively. all sequences present the character ... | 2011 | 21421056 |
| older triploid fish retain impaired reproductive endocrinology in the european sea bass dicentrarchus labrax. | this paper reports on an evaluation of growth, gonadal development and reproductive endocrinology of older triploid (3n) european sea bass dicentrarchus labrax in comparison with their diploid (2n) counterparts throughout their fifth and seventh annual cycle of life. while older triploids retained impaired reproductive endocrinology, a sexually related dimorphic growth was observed with 3n females attaining the largest sizes. comparisons of some body indexes showed that 3n females had a signific ... | 2009 | 20738514 |
| detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish. | epithelial surfaces of fish, such as the gut, skin and gills, comprise a large surface area for possible pathogen invasion. antimicrobial peptides (amps), innate immunity components, play a significant role in protecting fish. piscidins are a family of amps. in this study, we detected the presence of the recently discovered piscidin 4 via bug blot, western blot, elisa and/or immunohistochemistry in striped bass (morone saxatilis), white bass (m. chrysops), european seabass (dicentrarchus labrax) ... | 2010 | 19913049 |
| spermiogenic nuclear protein transitions and chromatin condensation. proposal for an ancestral model of nuclear spermiogenesis. | we have chosen three species (sparus aurata, dicentrarchus labrax, and monodonta turbinata) that represent different transition patterns in the composition and structure of spermiogenic nuclei. the transition patterns of these species are representative of spermiogenesis in a large number of animal species. we analyze: (a) nuclear protein exchange; (b) chromatin condensation pattern; and (c) histone acetylation during spermiogenic development. in the simplest spermiogenesis histones and nucleoso ... | 2009 | 19132734 |
| linking the genomes of nonmodel teleosts through comparative genomics. | recently the genomes of two more teleost species have been released: the medaka (oryzias latipes), and the three-spined stickleback (gasterosteus aculateus). the rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. with the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. furthermore, comparison of low coverage gene map ... | 2008 | 18297360 |
| cellular localization of insulin-like growth factor-ii protein in the sea bass (dicentrarchus labrax) from hatching to adult. | the cellular localization of igf-ii protein was investigated during larval and postlarval developmental stages of sea bass (dicentrarchus labrax) by immunohistochemistry using antisera raised against sparus aurata igf-ii. at hatching, igf-ii immunoreactivity was already present in the skin, developing intestine and skeletal muscle. during larval life igf-ii protein was also observed in heart musculature, in kidney and gill epithelia as well as in liver. in fry skeletal muscle a moderate igf-ii i ... | 2008 | 18283636 |
| effect of the pineal hormone melatonin on teleost fish phagocyte innate immune responses after in vitro treatment. | although neuroendocrine-immune system interaction has been shown in teleost fish, no study has evaluated the role of melatonin (mel) on fish immune response even considering that it is affected by the photoperiod. gilthead seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) head-kidney leucocytes were incubated with mel (0-control-, 20 pm-400 microm) and leucocyte viability and main innate cellular immune parameters were evaluated. overall, seabream and sea bass head-kidney leucoc ... | 2007 | 17620289 |
| effect of photoperiod on the fish innate immune system: a link between fish pineal gland and the immune system. | the pineal gland via its secretory product, melatonin, influences the light-dark rhythm in most vertebrates including fish. apart from the information concerning this circadian rhythm, the interrelation of the melatonin with other physiological processes has not been considered in fish. thus, we evaluated the changes in the humoral innate immune system of seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) specimens exposed to a constant light-dark photoperiod (12 hr l:12 hr d). s ... | 2006 | 16948787 |
| protein and arginine requirements for maintenance and nitrogen gain in four teleosts. | besides being an indispensable amino acid for protein synthesis, arginine (arg) is also involved in a number of other physiological functions. available data on the quantitative requirement for arg in different teleosts appear to show much variability. so far, there are very limited data on the maintenance requirements of indispensable amino acids (iaa) in fish. in the present study, we compared n and arg requirements for maintenance and growth of four finfish species: rainbow trout (oncorhynchu ... | 2002 | 12010584 |
| effects of beta-naphthoflavone on the cytochrome p450 system, and phase ii enzymes in gilthead seabream (sparus aurata). | the effect of beta-naphthoflavone (beta-nf) on several catalytic activities of cytochrome p450 (cyp) and phase ii enzymes putatively controlled by [ah]-receptor activation in the liver, heart and kidney of gilthead seabream, was investigated. in the liver, beta-nf treatment [intraperitoneal injection (i.p.) 50 mg/kg] resulted in an increase of cyp content, immunoreactive cyp 1a and methoxyresorufin-o-demethylase (merod), pentoxyresorufin o-depentylase (prod) and ethoxyresorufin-o-deethylase (ero ... | 2001 | 11544149 |
| proteins from fish eggs that protect dna from acid precipitation and inhibit dna synthesis. | we partially characterized proteins that inhibit dna acid precipitation from various fish eggs (sparus aurata, dicentrarchus labrax, mugil cephalus and zeus faber). the active proteins were purified by acetone fractionation. the activity was found to be heat resistant. of bivalent cations tested only co(2+) and cu(2+) exerted a profound promoting effect in the activity from all fish. the protein fraction from sparus aurata inhibited dna synthesis in pcr performed by different dna polymerases. th ... | 2001 | 11489434 |
| cytoarchitectonic study of the brain of a perciform species, the sea bass (dicentrarchus labrax). ii. the diencephalon. | the cytoarchitecture of nuclei in the preoptic area, ventral thalamus, dorsal thalamus, epithalamus, hypothalamus, posterior tuberculum, synencephalon, and pretectum and the accessory optic nuclei was analyzed in a perciform teleost, the sea bass dicentrarchus labrax, by using serial sections stained with cresyl-violet. in general, the cytoarchitecture of the preoptic area, ventral and dorsal thalamus, epithalamus, and synencephalon resembles the histological pattern of other teleosts. however, ... | 2001 | 11223930 |
| hepatic steatosis in artificially fed marine teleosts. | in a comparison of livers in fish (sparus auratus and dicentrarchus labrax) feeding on natural sources of food with livers of artificially fed animals, a much higher c18:1/c22:6 ratio was observed in the latter. staining livers with oil red o showed extensive steatosis in artificially fed fish, but not in those naturally fed. juvenile artificially fed fish showed a more extensive steatosis and a higher mortality rate. in steatotic fish fed a natural diet for 2 months, the liver exhibited extensi ... | 1998 | 21361972 |
| histochemistry of carbohydrates, proteins and lipids during swimbladder development in seabream, sparus aurata and seabass, dicentrarchus labrax. | some histochemical aspects of carbohydrates, proteins and lipids were studied in the swimbladder of seabream, sparus aurata and seabass, dicentrarchus labrax. in both species, swimbladder development occurs in two stages: (1) inflation and (2) expansion, respectively. no important differences in histochemical distribution of glucidic, proteic and lipidic macromolecules were observed during the swimbladder development. glycoproteins containing mannose and/or glucose sugar are present in the swimb ... | 1997 | 9491314 |
| nonspecific cell-mediated cytotoxicity in the seawater teleosts (sparus aurata and dicentrarchus labrax): ultrastructural study of target cell death mechanisms. | in higher vertebrates, cytolytic lymphocytes lyse their targets by the mechanisms of cell death called necrosis and apoptosis. however, the mechanisms of target cell death mediated by fish cytotoxic cells have not been established. we report the ultrastructure of target cell death mediated by the seawater teleosts gilthead seabream (sparus aurata) and sea bass (dicentrarchus labrax). | 1996 | 8694285 |
| fish plasma lipoproteins--comparative observations in serranides and sparides. | 1. diet, time from last feeding, temperature, season and sexual stage are some of the factors influencing the lipoprotein pattern. 2. keeping these factors constant species-specific differences observed among lipoprotein patterns of sparus aurata, puntazzo puntazzo, diplodus sargus, diplodus vulgaris and dicentrarchus labrax are discussed. 3. feeding habits and therefore lipid absorption and the rate of lipoprotein maturation process are the factors determining the observed differences. | 1991 | 1764905 |
| molecular evidence for the existence of species complexes within macvicaria gibson & bray, 1982 (digenea: opecoelidae) in the western mediterranean, with descriptions of two new species. | morphological and molecular characterisation of specimens of macvicaria spp. (digenea: opecoelidae) from five species of mediterranean sparids (teleostei: sparidae) sampled in the bizerte lagoon and the bay of bizerte (tunisia) revealed the presence of two species complexes designated as "obovata" and "crassigula" groups. detailed comparative morphological and phylogenetic analyses revealed that two of the specimen sets, one from each complex, represent species new to science. m. maamouriae n. s ... | 2015 | 26063299 |
| host-parasite relationship of ceratomyxa puntazzi n. sp. (myxozoa: myxosporea) and sharpsnout seabream diplodus puntazzo (walbaum, 1792) from the mediterranean with first data on ceratomyxid host specificity in sparids. | sparidae are economically important fishes to both, fisheries and aquaculture in the mediterranean. species diversification is an important strategy for the development of mediterranean aquaculture. one of the species recently introduced is the sharpsnout seabream diplodus puntazzo (walbaum, 1792). during a parasitological study of fish from the gulf of valencia and the mar menor (spain), myxozoan spores belonging to the genus ceratomyxa were found in the gall bladder of d. puntazzo. a morpholog ... | 2011 | 21680098 |
| salinity-dependent copper accumulation in the guppy poecilia vivipara is associated with ctr1 and atp7b transcriptional regulation. | copper (cu) accumulation and regulation of key-genes involved in cu homeostasis were evaluated in freshwater- and saltwater-acclimated guppies poecilia vivipara. fish were exposed (96h) to environmentally relevant concentrations of dissolved cu (0, 5.0, 9.0 and 20.0μg/l). in freshwater guppies, gill and liver cu accumulation was dependent on cu concentration in the exposure medium. in saltwater guppies, this dependence was observed only in the gut. these findings indicate that cu accumulation wa ... | 2014 | 24813262 |
| temperature and the expression of seven muscle-specific protein genes during embryogenesis in the atlantic cod gadus morhua l. | seven cdna clones coding for different muscle-specific proteins (msps) were isolated from the fast muscle tissue of atlantic cod gadus morhua l. in situ hybridization using crna probes was used to characterize the temporal and spatial patterns of gene expression with respect to somite stage in embryos incubated at 4 degrees c, 7 degrees c and 10 degrees c. myod transcripts were first observed in the presomitic mesoderm prior to somite formation, and in the lateral compartment of the forming somi ... | 2003 | 12909700 |
| using a concentrate of phenols obtained from olive vegetation water to preserve chilled food: two case studies. | phenols are plant metabolites characterised by several interesting bioactive properties such as antioxidant and bactericidal activities. in this study the application of a phenols concentrate (pc) from olive vegetation water to two different fresh products - gilt-head seabream (sparus aurata) and chicken breast - was described. products were treated in a bath of pc (22 g/l; chicken breast) or sprayed with two different solutions (l1:0.75 and l2:1.5 mg/ml; seabream) and then stored under refriger ... | 2016 | 27800444 |
| the effect of live feeds bathed with the red seaweed asparagopsis armata on the survival, growth and physiology status of sparus aurata larvae. | larval rearing is affected by a wide range of microorganisms that thrive in larviculture systems. some seaweed species have metabolites capable of reducing the bacterial load. however, no studies have yet tested whether including seaweed metabolites on larval rearing systems has any effects on the larvae development. this work assessed the development of sparus aurata larvae fed preys treated with an asparagopsis armata product. live prey, brachionus spp. and artemia sp., were immersed in a solu ... | 2017 | 28315161 |
| ultrastructure and phylogeny of ceratomyxa sp. (myxosporea), a parasite infecting sparus aurata (teleostei) in a portuguese fish farm. | | 2015 | 26227725 |
| ultrastructure and phylogeny of ceratomyxa auratae n. sp. (myxosporea: ceratomyxidae), a parasite infecting the gilthead seabream sparus aurata (teleostei: sparidae). | a new myxosporean parasite is described from the gall bladder of the gilthead seabream sparus aurata in a southern portuguese fish farm, with basis on light and transmission electron microscopy, as well as in molecular procedures. in the bile, young and mature mono- to disporic plasmodia were elliptical and presented smooth surface membranes. crescent-shaped myxospores measured 6.7±0.7 (5.3-7.6) μm in length and 27.0±3.0 (19.7-31.2) μm in thickness. the myxospore wall was constituted by two symm ... | 2015 | 25892564 |
| ortholinea auratae n. sp. (myxozoa, ortholineidae) infecting the urinary bladder of the gilthead seabream sparus aurata (teleostei, sparidae), in a portuguese fish farm. | a new myxosporean species, ortholinea auratae n. sp., is described from the gilthead seabream, sparus aurata linnaeus, 1758 (teleostei, sparidae) from a fish farm in algarve, portugal. plasmodia and spores were found in the urinary bladder and, less frequently, in the posterior kidney. plasmodia were polymorphic, presenting an irregular cellular membrane due to the presence of several peripheral projections, which in turn were covered by a glycocalyx-like sheet. mature spores were subspherical i ... | 2014 | 24985497 |