| improved performance and quantitative detection of copro-antigens by a monoclonal antibody based elisa to diagnose human opisthorchiasis. | copro-antigen detection has been advocated as a promising method for diagnosis of opisthorchiasis, particularly in people that harbored light infection or have had recent drug treatment. this study aimed to improve performance of a monoclonal antibody-based enzyme-linked immunosorbent assay (mab-elisa) for detection of opisthorchis viverrini copro-antigen and assess the correlation between copro-antigen and intensity of infection. four different treatment methods of 71 samples from the lawa ende ... | 2013 | 24055716 |
| combined effects of polymorphisms of dna-repair protein genes and metabolic enzyme genes on the risk of cholangiocarcinoma. | although opisthorchis viverrini is a risk factor for cholangiocarcinoma, not all the infected individuals develop cholangiocarcinoma. we investigated whether the base excision repair enzyme gene polymorphisms with differentiated repair capacities of inflammation-related deoxyribonucleic acid damage may play a key role and such possible effects from those genes may be increased or diminished in co-existence of polymorphisms of metabolic enzymes, including glutathione-s-transferases mu 1 and gluta ... | 2013 | 24049014 |
| increase of exostosin 1 in plasma as a potential biomarker for opisthorchiasis-associated cholangiocarcinoma. | a proteomic-based approach was used to search for potential markers in the plasma of hamsters in which cholangiocarcinoma (cca) was induced by opisthorchis viverrini infection and n-nitrosodimethylamine treatment. the plasma proteins of cca-induced hamsters were resolved by 1-d page, digested by trypsin, and analyzed by lc-ms/ms. from the criteria of protein id scores >15 and an overexpression of at least three times across all time points, 37 proteins were selected. these overexpressed proteins ... | 2014 | 24018821 |
| anti-apoptotic phenotypes of cholestan-3β,5α,6β-triol-resistant human cholangiocytes: characteristics contributing to the genesis of cholangiocarcinoma. | the oxysterols cholestan-3β,5α,6β-triol (triol) and 3-keto-cholest-4-ene (3k4) are increased in opisthorchis viverrini-associated hamster cholangiocarcinoma and induce dna damage and apoptosis via a mitochondria-dependent mechanism in mmnk-1 human cholangiocytes. based on these observations, we hypothesized that chronic exposure of cholangiocytes to these pathogenic oxysterols may allow a growth advantage to a subset of these cells through selection for resistance to apoptosis, thereby contribut ... | 2013 | 23959098 |
| mri and (1)h mrs evaluation for the serial bile duct changes in hamsters after infection with opisthorchis viverrini. | a 3t mr scanner was used to investigate the relationship between the alteration of bile duct lesions and the hepatic metabolic changes in hamsters infected with opisthorchis viverrini by using 3t mri and (1)h mr spectroscopy. animals were divided into control and infected groups. five normal hamsters were used as control; fifty-five hamsters were infected with o. viverrini to induce bile duct lesions and hepatic metabolic changes. t2-weighted image sequence in three orthogonal planes were conduc ... | 2013 | 23891436 |
| does a combination of opisthorchiasis and ethyl alcohol consumption enhance early cholangiofibrosis, the risk of cholangiocarcinoma? | combination of opisthorchis viverrini infection and other factors could drive cholangiocarcinoma (cca) development in southeast asia. however, other cca factors are obscure. alcohol consumption is well known in the risk for several cancers, but there is no report in cca development. therefore, the present study was to clarify whether drinking alcohol increases the liver pathology of opisthorchis viverrini (ov) infection which may be the cca risk. experimental syrian hamsters were divided into tw ... | 2013 | 23807659 |
| a follow-up study of opisthorchis viverrini infection after the implementation of control program in a rural community, central thailand. | opisthorchis viverrini infection is still one of the public health problems in thailand. our recent cohort study conducted in a rural community in central thailand showed that the incidence rate of o. viverrini infection in 2002-2004 was 21.6/100 person-years. conventional control activities including case diagnosis and treatment, hygienic defecation promotion and health education focusing on avoiding raw fish consumption was implemented. this study aimed to re-assess the status of infection aft ... | 2013 | 23786863 |
| annexin a1: a new immunohistological marker of cholangiocarcinoma. | to evaluate a new immunohistological marker, annexin a1 (anxa1), in cholangiocarcinoma (cca) and hepatocellular carcinoma (hcc). | 2013 | 23674846 |
| tumor-related gene changes in immunosuppressive syrian hamster cholangiocarcinoma. | the results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed syrian hamsters using prednisolone (p); normal syrian hamsters administered n-nitrosodimethylamine (nd); immunosuppressed syrian hamsters administered n-nitrosodimethylamine (ndis); normal syrian hamsters infected with opisthorchis ... | 2013 | 23645518 |
| [opisthorchiasis: tradition and innovation]. | the purpose of the review: present new data on the causative agent, pathogenesis, diagnosis and treatment of opisthorchiasis. | 2012 | 23402155 |
| proteomic analysis to identify plasma orosomucoid 2 and kinesin 18a as potential biomarkers of cholangiocarcinoma. | proteomic analysis was performed to search for the diagnostic biomarkers of the early stage of cholangiocarcinoma (cca). for this purpose, cca was experimentally induced in hamsters by the combination of n-nitrosodimethylamine (ndma) treatment and opisthorchis viverrini (ov) infection. pooled plasma of normal control, ndma-treated, ov-infected and ov+ndma (on) treated group was separated by 1-d page, and the trypsin-digested bands were analyzed with lc-ms/ms. among 82 overexpressed proteins, the ... | 2012 | 23396253 |
| human contact influences the foraging behaviour and parasite community in long-tailed macaques. | human–wildlife interactions have reached unprecedented levels, and humans are influencing the earth’s ecosystems more rapidly and extensively than ever before. this situation is cause for serious concern, especially since disease interactions between wildlife and humans have been recognized as major conservation threats. in this study, long-tailed macaques, macaca fascicularis, from 2 forest parks located in north-eastern thailand were investigated to determine the influence of habitat modificat ... | 2013 | 23363557 |
| oxidized alpha-1 antitrypsin as a predictive risk marker of opisthorchiasis-associated cholangiocarcinoma. | the oxidized alpha-1 antitrypsin (ox-a1at) is one modified form of a1at, generated via oxidation at its active site by free radicals released from inflammatory cells which subsequently are unable to inhibit protease enzymes. the presence of ox-a1at in human serum has been used as oxidative stress indicator in many diseases. as oxidative/nitrative damage is one major contributor in opisthorchiasis-driven cholangiocarcinogenesis, we determined a1at and ox-a1at expression in human cholangiocarcinom ... | 2013 | 23188705 |
| α-tocopherol and lipid profiles in plasma and the expression of α-tocopherol-related molecules in the liver of opisthorchis viverrini-infected hamsters. | opisthorchis viverrini infection induces inflammation-mediated oxidative stress and liver injury, which may alter α-tocopherol and lipid metabolism. we investigated plasma α-tocopherol and lipid profiles in hamsters infected with o. viverrini. levels of α-tocopherol, cholesterol, and low-density lipoprotein increased in the acute phase of infection. in the chronic phase, α-tocopherol decreased, while triglyceride and very low-density lipoprotein increased. notably, high-density lipoprotein decre ... | 2013 | 23178928 |
| population genetic structuring in opisthorchis viverrini over various spatial scales in thailand and lao pdr. | khon kaen province in northeast thailand is known as a hot spot for opisthorchiasis in southeast asia. preliminary allozyme and mitochondrial dna haplotype data from within one endemic district in this province (ban phai), indicated substantial genetic variability within opisthorchis viverrini. here, we used microsatellite dna analyses to examine the genetic diversity and population structure of o. viverrini from four geographically close localities in khon kaen province. genotyping based on 12 ... | 2012 | 23166853 |
| risk factors for opisthorchis viverrini infection among schoolchildren in lao pdr. | a matched-pair case-control study was conducted to identify factors associated with opisthorchis viverrini infection among primary schoolchildren to develop preventive education. a house-to-house interview of the guardians of 118 children (59 cases and 59 controls) was conducted to collect information about theirs and their children's fish eating habits of 10 locally available fish species. the guardians' knowledge and attitudes about opisthorchis viverrini infection and socio-economic status we ... | 2012 | 23077836 |
| viable metacercariae of opisthorchis viverrini in northeastern thai cyprinid fish dishes--as part of a rational program for control of o. viverrini-associated cholangiocarcinoma. | among the various types of chronic helminthic infections, only schistosomiasis hematobium, opisthorchiasis, and clonorchiasis in a long-standing inflammation are found to be causative agents of cancer. opisthorchiasis and opisthorchis viverrini-associated bile duct cancer are prevalent among the residents in northeastern thailand, who prefer consuming raw or improperly cooked cyprinid fish. although numerous studies have reported on the presence of o. viverrini metacercariae in freshwater cyprin ... | 2013 | 23052784 |
| diagnosis of early infection and post chemotherapeutic treatment by copro-dna detection in experimental opisthorchiasis. | opisthorchis viverrini is considered as a carcinogenic parasite which is responsible for cholangiocarcinoma in southeast asia. effective treatment and control of the parasite to reduce the risk of cancer requires efficient diagnostic methods. because of the limitations involved in human studies, the present work is aimed at comparing diagnostic performance of copro-dna detection by pcr and fecal examination by formalin-ethyl acetate concentration technique (fect) during the course of o. viverrin ... | 2013 | 23052766 |
| prevalence of opisthorchis viverrini infection in humans and fish in kratie province, cambodia. | opisthorchis viverrini is a medically important foodborne parasite in the indochina peninsula. in cambodia, the prevalence of this trematode has been reported in takeo province, but not in other areas. in this study, we investigated the prevalence of o. viverrini infection among people in seven riparian villages along the mekong river, kratie province. we also examined the status of metacercarial infection in fish hosts. fecal specimens were collected from 2101 residents and schoolchildren, and ... | 2012 | 22935318 |
| efficacy of praziquantel against schistosoma mekongi and opisthorchis viverrini: a randomized, single-blinded dose-comparison trial. | schistosomiasis and opisthorchiasis are of public health importance in southeast asia. praziquantel (pzq) is the drug of choice for morbidity control but few dose comparisons have been made. | 2012 | 22848766 |
| severe morbidity due to opisthorchis viverrini and schistosoma mekongi infection in lao people's democratic republic. | we assessed morbidity due to opisthorchis viverrini and schistosoma mekongi infections in 243 individuals in lao people's democratic republic. morbidity was associated with o. viverrini infection intensity. coinfection with s. mekongi resulted in excess risk of liver fibrosis and left liver lobe enlargement. the high public health impact of opisthorchiasis warrants control. | 2012 | 22670046 |
| elevated plasma il-6 associates with increased risk of advanced fibrosis and cholangiocarcinoma in individuals infected by opisthorchis viverrini. | opisthorchis viverrini is considered among the most important of the food-borne trematodes due to its strong association with advanced periductal fibrosis and bile duct cancer (cholangiocarcinoma). we investigated the relationship between plasma levels of interleukin (il)-6 and the risk of developing advanced fibrosis and bile duct cancer from chronic opisthorchis infection. we show that il-6 circulates in plasma at concentrations 58 times higher in individuals with advanced fibrosis than age, s ... | 2012 | 22629477 |
| ultrasonography as a tool for monitoring the development and progression of cholangiocarcinoma in opisthorchis viverrini/ dimethylnitrosamine-induced hamsters. | cholangiocarcinoma (cca) is the most common cancer in northeastern thailand. at present, effective diagnosis of cca either in humans or animals is not available. monitoring the development and progression of cca in animal models is essential for research and development of new promising chemotherapeutics. ultrasonography has been widely used for screening of bile duct obstruction in cca patients. in this study, we preliminarily investigated the applicability of ultrasonography to monitor the dev ... | 2012 | 22502719 |
| alteration of galectin-1 during tumorigenesis of opisthorchis viverrini infection-induced cholangiocarcinoma and its correlation with clinicopathology. | galectin-1 is a beta-galactoside-binding lectin to function in cell adhesion, proliferation, differentiation, and might be involved in tumor progression and metastasis. in the present study, the expression kinetics of galectin-1 during the tumorigenesis of a parasite opisthorchis viverrini infection-induced cholangiocarcinoma (cca) was investigated in model animal hamsters, and the expression was confirmed in human cca cases. it was found that galectin-1 was overexpressed at mrna and protein lev ... | 2012 | 22373585 |
| spatial distribution of, and risk factors for, opisthorchis viverrini infection in southern lao pdr. | opisthorchis viverrini is a food-borne trematode species that might give rise to biliary diseases and the fatal cholangiocarcinoma. in lao pdr, an estimated 2.5 million individuals are infected with o. viverrini, but epidemiological studies are scarce and the spatial distribution of infection remains to be determined. our aim was to map the distribution of o. viverrini in southern lao pdr, identify underlying risk factors, and predict the prevalence of o. viverrini at non-surveyed locations. | 2012 | 22348157 |
| comparison of novel and standard diagnostic tools for the detection of schistosoma mekongi infection in lao people's democratic republic and cambodia. | given the restricted distribution of schistosoma mekongi in one province in lao people's democratic republic (lao pdr) and two provinces in cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. however, sensitive diagnostic tools will be required to determine whether elimination has been achieved. we compared several standard and novel diagnostic tools in s. mekongi-ende ... | 2017 | 28793922 |
| lineage-specific expansion and loss of tyrosinase genes across platyhelminths and their induction profiles in the carcinogenic oriental liver fluke, clonorchis sinensis. | tyrosinase provides an essential activity during egg production in diverse platyhelminths by mediating sclerotization of eggshells. in this study, we investigated the genomic and evolutionary features of tyrosinases in parasitic platyhelminths whose genomic information is available. a pair of paralogous tyrosinases was detected in most trematodes, whereas they were lost in cyclophyllidean cestodes. a pseudophyllidean cestode displaying egg biology similar to that of trematodes possessed an ortho ... | 2017 | 28583212 |
| codon usage patterns of tyrosinase genes in clonorchis sinensis. | codon usage bias (cub) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. in this study, genetic indices associated with cub, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. the ... | 2017 | 28506040 |
| first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar. | opisthorchis viverrini is endemic in the south east asian region, especially in cambodia, lao people's democratic republic, vietnam and thailand, but there have been no previous records from myanmar. during stool surveys of rural populations in three regions of lower myanmar, opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. dna was extracted from these positive stool samples and a portion o ... | 2017 | 28472153 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis, and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsis ... | 2015 | 28741473 |
| opisthorchiasis and cholangiocarcinoma in southeast asia: an unresolved problem. | the prevalence of cholangiocarcinoma (cca) in southeast asia is much higher than other areas of the world. eating raw, fermented, or undercooked cyprinid fish, infected with the liver fluke, opisthorchis viverrini sensu lato (sl), results in chronic biliary inflammation, periductal fibrosis, and increased cancer risk. there may be associated glomerulonephritis. the process of infection is difficult to disrupt because eating practices have proven extremely difficult to change, and the life cycle ... | 2017 | 28848361 |
| the battle of worldviews: a case study of liver fluke infection in khon kaen, thailand. | control efforts to reduce infection from the parasitic flatworm opisthorchis viverrini have progressed through understanding the epidemiology of opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. yet the problem persists. the case study method was used to examine the fundamental cause of the liver fluke infection problem. evidence shows that the liver fluke-infected population does not ... | 2017 | 28795594 |
| prospects and challenges towards sustainable liver fluke control. | the liver fluke opisthorchis viverrini (ov) is endemic in southeast asia where more than 10 million people are estimated to be infected. the infection is associated with several hepatobiliary diseases, including cholangiocarcinoma (cca). northeast thailand is a hotspot for ov transmission, and, despite extensive public health prevention campaigns led by the government, the prevalence of ov infection is still high. high infection rates result from cultural and ecological complexities where wet-ri ... | 2017 | 28754415 |
| ultrastructural characters of the spermatozoon of the liver fluke opisthorchis viverrini (poirier, 1886) (opisthorchiidae). | the present study records the ultrastructural organization of the mature spermatozoon of opisthorchis viverrini by means of transmission electron microscopy. the spermatozoon of o. viverrini is a filiform cell, tapered at both extremities. it exhibits the characteristics of type iv spermatozoon of digeneans, namely with two axonemes of the 9+'1' trepaxonematan pattern, external ornamentation of the plasma membrane associated with cortical microtubules that are in the posterior part of the anteri ... | 2017 | 28725936 |
| subsets of inflammatory cytokine gene polymorphisms are associated with risk of carcinogenic liver fluke opisthorchis viverrini-associated advanced periductal fibrosis and cholangiocarcinoma. | opisthorchis viverrini infection induces chronic inflammation, and a minor proportion of infected individuals develop advanced periductal fibrosis (apf) and cholangiocarcinoma (cca). inflammatory cytokines and/or their gene polymorphisms may link to these biliary pathologies. we therefore investigated associations among cytokine gene polymorphisms and cytokine production in 510 thai cases infected with o. viverrini who presented with apf+ or apf-, as established by abdominal ultrasonography as w ... | 2017 | 28719954 |
| simplified techniques for killing the carcinogenic, opisthorchis viverrini metacercariae in cyprinid fish | consumption of fluke-free fish is the most important factor in controlling opisthorchis viverrini (ov) infection in endemic areas such as northeast thailand and thereby reducing the risk of cholangiocarcinoma. cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. we investigated the food preparation process, using freezing, heating and fermentation to kill ov metacercariae in fish. uncooked cyprinid fish infected w ... | 2017 | 28669159 |
| present situation of opisthorchiasis in vientiane capital, lao peoples' democratic republic. | opisthorchiasis is the commonest liver disease caused by opisthorchis viverrini in thailand, lao people's democratic republic (pdr) and cambodia, which will be a major cause of future human cholangiocarcinoma in these regions. in this short review, we describe the epidemic aspects of this parasite infection and the outline of results of our recent surveys of the fluke in humans and its intermediate hosts in vientiane capital, where information on the opisthorchiasis epidemic is insufficient. we ... | 2017 | 28552889 |
| development of a potent wound healing agent based on the liver fluke granulin structural fold. | granulins are a family of protein growth factors that are involved in cell proliferation. an orthologue of granulin from the human parasitic liver fluke opisthorchis viverrini, known as ov-grn-1, induces angiogenesis and accelerates wound repair. recombinant ov-grn-1 production is complex and poses an obstacle for clinical development. to identify the bioactive region(s) of ov-grn-1, four truncated n-terminal analogues were synthesized and characterized structurally using nmr spectroscopy. pepti ... | 2017 | 28425707 |
| protein kinases as targets for opisthorchis viverrini- associated cholangiocarcinoma therapy. | protein kinases are enzymes that catalyze the transfer of phosphate from atp to the serine/threonine or tyrosine residues of target molecules. these are key important mediators in a signaling cascade involved in several biological processes. dysregulation of their activity has been found in various tumors. from our increased understanding of kinase structure and activation mechanisms there emerged new strategies for targeting kinase in cancer treatment. nowadays, kinase specific inhibitors are d ... | 2017 | 28699535 |
| nimotuzumab inhibits cholangiocarcinoma cell metastasis via suppression of the epithelial-mesenchymal transition process. | changes in epidermal growth factor receptor (egfr) are commonly found in cancer progression, signaling a poor outcome in patients. in the present study, we aimed to investigate whether nimotuzumab could be of benefit for cholangiocarcinoma (cca) treatment. | 2017 | 28668850 |
| the role of domestic dogs in the transmission of zoonotic helminthes in a rural area of mekong river basin. | dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. some canine helminth parasites can cause significant clinical diseases in humans as opisthorchis viverrini causing cholangiocarcinoma in southeast asian countries. in this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in savannakhet, lao-pdr, a typical mekong basin area. dog ow ... | 2017 | 28426425 |