| zymodemes of entamoeba histolytica in dhaka, bangladesh. | stool samples containing entamoeba histolytica were obtained from two sources: an urban hospital and an urban slum. using the enzyme patterns of three enzymes (e.c.2.7.1.1 hexokinase, e.c.5.3.1.9 glucose phosphate isomerase and e.c.2.7.5.1 phosphoglucomutase) stained after cellulose acetate electrophoresis, four zymodemes (i, ii, xiv and xvi) were identified in 71 isolates. zymodemes considered to be pathogenic (ii and xiv) were identified from 31 of 34 isolates from the urban hospital, and thes ... | 1990 | 2150165 |
| [cloning and expression in e coli of dna from entamoeba histolytica: detection using antibodies purified with fixed trophozoites]. | | 1990 | 2152102 |
| role of prostaglandins and calcium in the effects of entamoeba histolytica on colonic electrolyte transport. | we have previously shown that entamoeba histolytica lysates contain the neurohormones serotonin, neurotensin, immunoreactive substance p, and probably acetylcholine, and that amebic lysates inhibit sodium and chloride absorption and stimulate chloride secretion in the rat descending colon as measured by the ussing chamber-voltage clamp technique. we now demonstrate that these transport effects have both calcium-dependent and calcium-independent components. in addition, arachidonic acid metabolit ... | 1990 | 2155844 |
| specific features of phosphomonoesterase of entamoeba histolytica (nih-200). | axenically grown e. histolytica possess significant acid phosphatase activity. the km of the enzyme was found to be 7.1 x 10(-3) and was maximally active at ph 4.5. acid phosphatase activity was significantly inhibited by cu2+, cysteine and was activated by tartrate and fluoride. it was found that e. histolytica acid phosphatase differs in some properties as compared to the enzyme reported from other sources. | 1990 | 2164494 |
| antigens in electron-dense granules from entamoeba histolytica as possible markers for pathogenicity. | in vitro interaction of entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (edg) and stimulation of collagenolytic activity. purified edg contain 1.66 u of collagenase per mg of protein. thus, edg may participate in tissue destruction during invasive amebiasis. monoclonal antibodies (mabs) l1.1 and l7.1 reacted specifically with edg in enzyme-linked immunosorbent assay (elisa) and immunofluorescence and immunoelectron microscopy. mab l7.1 i ... | 1990 | 2174899 |
| characterisation of a repetitive dna family from entamoeba histolytica containing saccharomyces cerevisiae ars consensus sequences. | several repetitive dna families were identified in entamoeba histolytica dna digested with sau3ai. characterisation of one of these repetitive dna families showed the presence of multiple copies of saccharomyces cerevisiae autonomously replicating sequence (ars) core consensus sequences. the e. histolytica ars consensus sequences allowed a yeast-integrating plasmid, yip5, to replicate autonomously in s. cerevisiae. a 'bent dna' fragment was located in one member of this e. histolytica repetitive ... | 1990 | 2176638 |
| [diagnosis and therapy of amebic liver abscess]. | | 1990 | 2178901 |
| identification of a pathogenic isolate-specific 30,000-mr antigen of entamoeba histolytica by using a monoclonal antibody. | a monoclonal antibody (mab) produced against trophozoites of entamoeba histolytica strain hm-1:imss, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (z) patterns, i.e., z-ii, z-ii alpha-, z-ii (glucose phosphate isomerase: gamma +), z-vii, z-vii (glucose phosphate isomerase: alpha lack, gamma +), z-xi, z-xiv, and z-xix, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. in contrast, the mab failed to react ... | 1990 | 2180826 |
| appendiceal infection by entamoeba histolytica and strongyloides stercoralis presenting like acute appendicitis. | a 58-year-old male from puerto rico who was taking orally administered cortisone analogs for chronic obstructive pulmonary disease presented with fever, absolute eosinophilia, right lower quadrant pain, and rebound tenderness associated with strongyloides stercoralis infection of the appendix. a 37-year-old alcoholic male developed fever, right lower quadrant abdominal pain, and rebound tenderness because of infection of the appendix with entamoeba histolytica. these are the seventh reported cas ... | 1990 | 2185002 |
| entamoeba histolytica: pathogenic mechanisms, human immune response, and vaccine development. | | 1990 | 2186887 |
| [serodiagnosis of entamoebiasis]. | | 1990 | 2192131 |
| treatment of asymptomatic amebiasis in homosexual men. clinical trials with metronidazole, tinidazole, and diloxanide furoate. | in a randomized trial, the authors treated 42 asymptomatic homosexual men who had entamoeba histolytica in fecal specimens with either metronidazole or tinidazole (study 1). they treated both groups initially with 2,000 mg in a single dose, and in case of failure with 2,000 mg daily for 5 days. in cases of repeated failure, the authors prescribed a dosage of 500 mg of diloxanide furoate three times per day (tid) for 10 days. in a subsequent study (study 2) the authors treated 49 asymptomatic hom ... | 1990 | 2193407 |
| evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis. | a new bicolored latex agglutination amoeba test (bla) for detection of antibodies against entamoeba histolytica was evaluated for its practicability and diagnostic sensitivity and specificity. bla is rapid (5 min) and simple to perform. it requires only 20 microliters of a 1/3-diluted serum, 17 microliters of reagent, and a glass slide. reading of the test is easy because a positive result shows a green spot with a red surrounding edge. this bicolored pattern is easily distinguishable from the n ... | 1990 | 2199503 |
| [multiple amebic liver abscesses: ultrasound diagnosis and ultrasound-controlled puncture]. | amebic colitis and amebic liver abscess were diagnosed in a 45-year-old patient who had returned from thailand. ultrasound scans performed after starting treatment with metronidazole showed an abscess located above the right kidney that had enlarged to a maximum diameter of 9.7 cm. since rupture of this lesion was felt to be imminent, ultrasound-guided needle biopsy was performed and 300 ml of abscess fluid evacuated. the patient experienced rapid relief of his symptoms. the role of ultrasound i ... | 1990 | 2200116 |
| cdna sequence analysis of a 29-kda cysteine-rich surface antigen of pathogenic entamoeba histolytica. | a gamma gt11 cdna library was constructed from poly(u)-sepharose-selected entamoeba histolytica trophozoite rna in order to clone and identify surface antigens. the library was screened with rabbit polyclonal anti-e. histolytica serum. a 700-base-pair cdna insert was isolated and the nucleotide sequence was determined. the deduced amino acid sequence of the cdna revealed a cysteine-rich protein. dna hybridizations showed that the gene was specific to e. histolytica since the cdna probe reacted w ... | 1990 | 2201027 |
| missing infections in aids. | in north america and europe, the opportunistic infections from which patients with acquired immune deficiency syndrome (aids) frequently suffer are pneumocystis carinii pneumonia and mycobacterium avium-intracellulare: in central africa these infections are uncommon or non-existent. serious infections with entamoeba histolytica and strongyloides stercoralis would be expected to occur in aids patients: they do not. falciparum malaria might be expected to interact with hiv infection: it does not. ... | 1990 | 2201111 |
| binding of proteolytically-degraded human colonic mucin glycoproteins to the gal/galnac adherence lectin of entamoeba histolytica. | rat and human colonic mucin glycoproteins bind to the gal/galnac adherence lectin on the surface of entamoeba histolytica in vitro, thus inhibiting the organism from adhering to and lysing the target cells. human colonic mucin glycoproteins were isolated by sepharose 4b gel filtration chromatography, they were proteolytically degraded with trypsin, pronase, and papain, and the glycoprotein fractions were reisolated by sephacryl s-200 gel filtration chromatography. binding of the mucin glycoprote ... | 1990 | 2201583 |
| sexually transmitted diseases and enteric infections in the male homosexual population. | there are certain special considerations in the management of sexually transmitted diseases (stds) in homosexual men, with the impact of human immunodeficiency virus (hiv) infection on the presentation, diagnosis, and management of certain stds just becoming apparent recently. rectal and pharyngeal gonorrhea are usually asymptomatic and also more difficult to treat. the serological diagnosis of syphillis may be unreliable in acquired immunodeficiency syndrome (aids) patients, and hiv-seropositiv ... | 1990 | 2202414 |
| hepatopulmonary amoebiasis. efficacy of various treatment regimens containing dehydroemetine and/or metronidazole. | thirty three patients with hepatopulmonary amoebiasis were studied. pulmonary involvement was observed in 55%, pleuro-pulmonary lesions in 30%. typical chocolate coloured sputum was obtained in 24% and pleural fluid in 21% of patients. entamoeba histolytica were demonstrable in the sputum in only 6% and in the pleural fluid in 15%. the best therapeutic results were obtained with a combination of dehydroemetine and metronidazole. however, metronidazole was found to be nearly as effective as dehyd ... | 1990 | 2202709 |
| an immunogenic 30-kda surface antigen of pathogenic clinical isolates of entamoeba histolytica. | a 30-kda surface antigen was identified by western blots with human immune sera in all 15 isolates of e. histolytica from patients with invasive amebiasis (pathogenic) but not in 15 strains from asymptomatic patients (nonpathogenic). this antigen is highly immunogenic in naturally infected humans and was recognized by sera from 22 patients with invasive disease but not by sera from 13 patients harboring nonpathogenic strains. its surface location is supported by its differential extraction in th ... | 1990 | 2205653 |
| autonomous replication sequences in an extrachromosomal element of a pathogenic entamoeba histolytica. | entamoeba histolytica possesses a 24.5 kilobase plasmid-like molecule which encodes for the organism's ribosomal rnas. sequence analysis of this extrachromosomal element revealed the presence of at rich sequences which show homology to the origin of replication of other lower eucaryotes. an 802 bp fragment containing these sequences was cloned into a yeast shuttle vector lacking the origin of replication and the construct tested for its ability to replicate autonomously in yeast. mitotic stabili ... | 1990 | 2216723 |
| differences in circannual characteristics of the incidences of amebiasis and giardiasis. | entamoeba histolytica is the most common pathogenic intestinal parasite in méxico. prevalence rates of am vary widely from place to place, being higher in areas with poor sanitation, households and institutions, and among male homosexuals. early recognition and treatment may be necessary to prevent its spread in the community. intervention by preventive health education is indicated. the timing of such educational endeavours may be adjusted to any rhythmic and to that extent predictable changes ... | 1990 | 2217363 |
| [saf--an alternative fixation solution for parasitological stool specimens]. | a comparative parasitological study in 3 collectives using 2 different fixatives for stool samples (saf and mif) was carried out. the saf fixative consistently showed a significantly higher sensitivity for parasitic protozoa than the mif fixative (entamoeba histolytica 90.0% vs. 64.4%, giardia lamblia 97.7% vs. 64.4%), which was due to an increased detection of cysts of both species. no difference in sensitivity for helminths was found. the cheap, simple formula and, due to the absence of mercur ... | 1990 | 2218469 |
| [effects of saccharomyces boulardii yeast on trophozoites of entamoeba histolytica in vitro and in cecal amebiasis in young rats]. | lyophilized and rehydrated saccharomyces boulardii yeasts were administered to young rats previously inoculated into the cecum with entamoeba histolytica. the numbers of diseased young rats and the severity of the infection, assessed from the appearance of the cecum and its content of mucus and amebae, were significantly reduced by the treatment. the lesions observed resembled those seen in the untreated controls, although healing was faster in the treated animals. saccharomyces boulardii had no ... | 1990 | 2221756 |
| complete resolution of generalized eczema craquelé after treatment with metronidazole of occult amebiasis. | | 1990 | 2229511 |
| the zymodemes of entamoeba histolytica in new delhi, india. | the prevalence of various pathogenic and non-pathogenic zymodemes of entamoeba histolytica in new delhi, india was studied by characterising its isoenzyme patterns. non-pathogenic zymodeme i was found to be most common and was detected in 39 of 49 infections. the other non-pathogenic zymodemes detected were xvii and xviii in two cases each. the pathogenic zymodemes detected were ii (four cases), ii alpha- (alpha) (one case) and xiv (one case). approximately 72% of those harbouring non-pathogenic ... | 1990 | 2229988 |
| action of metronidazole on entamoeba histolytica: an ultrastructural study. | metronidazole (19 micrograms/ml) caused progressive increase in vacuolization in entamoeba histolytica cells with disintegration of its plasma membrane leading to almost complete disappearance of the latter within 3 hours. the drug also induced formation of helical ribosomal aggregates in the cytoplasm and disappearance of button like structures inside the nucleus. | 1990 | 2230021 |
| diagnosis of intestinal amebiasis using salivary iga antibody detection. | this investigation sought to determine whether detection of salivary iga antibodies to entamoeba histolytica could identify intestinal amebic infections among 223 school children. four groups of children were identified through coproparasitoscopic examination: e. histolytica as other parasites only (20%); and parasite-free (25%). the diagnostic accuracy of salivary iga antibodies to an e. histolytica membrane extract was 91.5% (sensitivity, 85%; specificity, 98%), maintaining high predictive val ... | 1990 | 2230266 |
| circulating e. histolytica antigens clear with successful treatment of amoebic liver abscess. | an antigen capture elisa was used to measure serum e. histolytica antigen and antibody levels in 23 patients with amebic liver abscess (ala), 15 patients with intestinal amebic infections (iai), and 20 normal controls. 16 of the 23 (70%) with ala had antigenemia. none of those with iai or the normal controls had e. histolytica antigen titers of greater than 1:4. all 23 of the patients with ala had positive antibody titers. three (20%) with iai and none of the normal controls had e. histolytica a ... | 1990 | 2230307 |
| elisa in detection of entamoeba histolytica antigens in stools. | the modified double antibody sandwich elisa was applied to detect e. histolytica antigens in the stool samples of 20 patients have amoebiasis (symptomatic or asymptomatic), 10 with intestinal parasites other than e. histolytica and 10 healthy individuals. the method was found to be sensitive (90%) and specific (85%). it is concluded that elisa could be applied for a large scale epidemiological study and immunodiagnosis of amoebiasis. | 1990 | 2230325 |
| health status of children living in a squatter area of manila, philippines, with particular emphasis on intestinal parasitoses. | in 1988 a study was performed in a squatter area of manila, philippines. three substudies were carried out: (1) an analysis of the reported health situation and the available health services for the population of smokey mountain, (2) a vaccination coverage survey and (3) a cross-sectional stool sample survey. respiratory infections, diarrhea, measles and malnutrition were the most frequently reported causes of ill-health and death. not only governmental but also various non-governmental health s ... | 1990 | 2237597 |
| [amebic liver abscess: 2 imported cases]. | two imported cases of amebic liver abscess are discussed with regard to laboratory, diagnostic and therapeutic peculiarities. the diagnostic role of ultrasonography and especially of specific serology is stressed, whereas in therapeutic terms, the efficacy of medical approach alone is emphasized even when complications until yesterday surgically treated occur. | 1990 | 2247696 |
| lysis of complement-sensitive entamoeba histolytica by activated terminal complement components. initiation of complement activation by an extracellular neutral cysteine proteinase. | activation of complement by entamoeba histolytica may be initiated by the extracellular 56-kd neutral cysteine proteinase which cleaves the alpha chain of c3. to determine the relationship between the fluid-phase activation of complement and our observation that only strains isolated from patients with invasive disease are resistant to complement-mediated lysis, we investigated the fate of c3 with recent amebic isolates. when 125i-c3 was incubated with trophozoites in serum, c3 in the fluid phas ... | 1990 | 2254446 |
| recognition of 29 kda surface-associated adhesive molecule of entamoeba histolytica by monoclonal antibodies. | monoclonal antibodies have been developed and used as specific probe to locate and identify a 29-kda molecule of axenic entamoeba histolytica trophozoites. monoclonal antibody produced by clone c8 (moab c8) strongly agglutinated the amoebic trophozoites. the immunofluorescence of live e. histolytica trophozoites and surface fluorescence of acetone-fixed trophozoites by moab c8 indicated existence of a 29-kda molecule on surface-associated plasma membrane of e. histolytica. the monoclonal antibod ... | 1990 | 2257172 |
| [acute, non-lithiasic, amebic cholecystitis]. | the estimated percentage of non calculous cholecystitis is 2-14%. in most cases it is difficult to identify the cause and in very few cases it is possible to establish the etiology. few cases are secondary to bacterial infections and the cause is very rarely a parasitic infection. we present a case of acute cholecystitis secondary to entamoeba histolytica infection; the frequency, etiopathogenic mechanism and treatment are discussed. | 1990 | 2257193 |
| intestinal amoebiasis and giardiasis in southern indian infants and children. | the role of entamoeba histolytica and giardia lamblia as causative agents of paediatric diarrhoea was studied in a southern indian population. relationship between infant feeding practices, co-existing malnutrition and the occurrence of intestinal amoebiasis and giardiasis was also examined. the subjects were 361 paediatric patients with acute diarrhoea and 70 hospitalized control children without diarrhoea. faecal samples from cases and controls were examined for the protozoal pathogens using f ... | 1990 | 2260173 |
| zymodemes of entamoeba histolytica isolated in the amazon and the north-east of brazil. | | 1990 | 2260174 |
| non-specific reactions in enzyme linked immunosorbent assays for serum antibody to entamoeba histolytica and giardia lamblia in non-endemic areas. | serum samples from 20 indian children with diarrhoea were compared with those from 20 children resident in the united kingdom who had been diagnosed as having ulcerative colitis, or crohn's disease, or indeterminate colitis using enzyme linked immunosorbent assays specific for entamoeba histolytica and giardia lamblia. more than 50% of the united kingdom patients had high igg responses in elisas for e histolytica and g lamblia. a confirmatory elisa showed that the british sera reacted specifical ... | 1990 | 2262568 |
| gnotobiotic, athymic mice; a possible system for the study of the role of bacteria in human amoebiasis. | two groups of 12 + 14 gnotobiotic, athymic mice were intracaecally injected with entamoeba histolytica strain hk9 and nih:200, respectively. two groups of 16 and 15 mice were given amoebae together with a pure strain of escherichia coli and a further two groups of 16 and 27 were given amoebae with a pure strain of clostridium perfringens. batches of 3-7 mice from each group were killed at intervals of 1-4 weeks. all the mice given nih:200 alone were found to be infected with trophozoites. of tho ... | 1990 | 2270046 |
| [nodular lymphoid hyperplasia of the intestine. clinico-pathologic characteristics in 11 cases]. | the clinical-pathological characteristics of 11 patients with intestinal nodular lymphoid hyperplasia (inlh) are described. five fulfilled the criteria for herman's syndrome and presented all or several of the following alterations: dysgammaglobulinemia, recurrent respiratory tract infections, sinusitis, pneumonia and giardiasis; of the remaining six cases, in five gammaglobulin levels were not quantified and in one they were normal. all the patients in this group suffered from recurrent pharyng ... | 1990 | 2270366 |
| status of intestinal parasite infections in inhabitants of palmar, guayas province, ecuador. | status of intestinal protozoan and helminthic infections was surveyed in mestizo population living in rural parish of palmar and its nearby recintos(villages). three of the surveyed villages were at the pacific coast and 5 villages were in inner pasture land, located about 100 km west of guayaquil, the second largest city in ecuador. one stool sample was examined by one cellophane thick smear for helminth ova and one direct smear stained with lugol's iodine solution for protozoan cysts. of 325 p ... | 1990 | 2271503 |
| development of simple and stable elisa for detection of copro antigen in intestinal amoebiasis. | a simple, sensitive and stable elisa (enzyme linked immunosorbent assay) was developed using rabbit antibody to fractionated entamoeba histolytica antigen for the detection of copro antigen in the faeces of individuals with intestinal amoebiasis. in this test none of the healthy individuals, all trophozoite positive, 40% cyst passers and 6% individuals living in endemic area showed the presence of copro antigen. elisa using polyclonal rabbit antibody could detect 1-5 trophozoites/well and 20-50 ... | 1990 | 2272654 |
| purification and partial characterization of the major cysteine protease from entamoeba invadens. | the purification and partial characterization of a major protease from the parasitic protozoon of reptiles, entamoeba invadens, is described. the enzyme has a molecular mass of 28 kda, and three distinct isoelectric points at ph 4.7, 5.7 and 6.3, respectively. as an endopeptidase the enzyme digests denatured protein substrates, such as azocasein with an optimal turnover rate at ph 4.8 with a temperature optimum of 48 degrees c. the protease exhibits exopeptidase activity towards arginine contain ... | 1990 | 2275719 |
| amebiasis in institutions for the mentally retarded in kanagawa prefecture, japan. | a parasitologic survey of 620 mentally retarded patients, institutionalized in five different facilities in kanagawa prefecture, revealed a high incidence (12.6%) of infection with entamoeba histolytica. a concomitant serologic survey, by the indirect fluorescent antibody test, gave a much higher incidence (26.5%). moreover, most zymodeme patterns of the amebae isolated from infected individuals were of a pathogenic type (zymodeme ii). our findings demonstrate that the mentally retarded in japan ... | 1990 | 2277448 |
| large-scale production of entamoeba histolytica trophozoites in polyxenic culture. | | 1990 | 2278073 |
| sexually transmitted diseases in leprosy patients in north and northeastern india. a futile search for human immunodeficiency virus antibody. | three-hundred-eighty-four leprosy patients were clinically examined for sexually transmitted diseases (std) in north and northeastern india, revealing a high incidence (5.2%) of std among them. eighteen males, one female, and one eunuch were found to have chancroid ulcer, gonococcal urethritis, lymphogranuloma inguinale, and primary chancre. of these patients, only 100, selected randomly, could be screened serologically for std due to treponema pallidum, herpes simplex (type 1 and 2), entamoeba ... | 1990 | 2280116 |
| magainin analogs effective against pathogenic protozoa. | the in vitro activities of magainin analogs against blastocystis hominis, entamoeba histolytica, and trypanosoma cruzi were assessed by protozoan morphological integrity and motility. the antiprotozoan activities in descending order were magainin b greater than g greater than h, the same order as the alpha-helix contents of the analogs. magainin b and g were effective against b. hominis, t. cruzi, and e. histolytica. | 1990 | 2285300 |
| [fundamental studies on serological diagnosis of amoebiasis. 2. application of antigen of entamoeba histolytica for complement fixation test to counterimmunoelectrophoresis; and clinical relevance of serological tests]. | antigen of entamoeba histolytica for complement fixation (cf) test was applied to counterimmunoelectrophoresis (cie), and the result was compared with that of cf test and enzyme-linked immunosorbent assay (elisa) in regard to sensitivity and specificity. besides, we studied antibody titers to e. histolytica in sera from acute and convalescent patients, and from patients with amoebic colitis and amoebic liver abscess. sera used were from patients with amoebic colitis, amoebic liver abscess and un ... | 1990 | 2286781 |
| specificity of glycosphingolipid recognition by entamoeba histolytica trophozoites. | the ability of purified glycosphingolipids to enhance liposome-stimulated entamoeba histolytica actin polymerization was assessed as a means of defining the specificity of mammalian cell membrane lipid glycan recognition by this parasite. synthetic liposomes containing a variety of individual glycosphingolipids bearing neutral, straight-chain oligomeric glycans with galactose or n-acetylgalactosamine termini stimulated rapid (90-s) polymerization of amoeba actin. glycans with terminal n-acetylgl ... | 1990 | 2294057 |
| gastric malignant lymphoma with superimposed amebiasis. | a case of primary b, large cell lymphoma of the stomach with superimposed amebiasis due to entamoeba histolytica is reported. this represents the second recorded case of such an association. a review of the subject and possible mechanisms of infection are discussed. | 1990 | 2296967 |
| comparison of stool microscopy and serology (enzyme linked immunosorbent assay) in epidemiology of amebiasis. | stools from 634 individuals from varanasi were examined for entamoeba histolytica (eh). serology was done in these subjects by enzyme linked immunosorbent assay (elisa) employing filter paper technique. stools were positive for eh in 16.9%, and serology in 15.9%. both the tests were positive in only 5.2%. in 72.4% both the tests were negative. in 11.7% of stool positive cases, serology was negative, and in 10.7% with positive serology stool examination did not reveal eh. a majority (92.5%) of st ... | 1990 | 2307498 |
| the 96-kilodalton antigen as an integral membrane protein in pathogenic entamoeba histolytica: potential differences in pathogenic and nonpathogenic isolates. | a surface antigen (eh-96) of entamoeba histolytica was demonstrated to be a plasma membrane antigen by immunoprecipitation of metabolically 35s-labeled antigen from live trophozoites, triton x-114 detergent extracts, and plasma membrane-enriched fractions prepared by concanavalin a membrane stabilization and differential centrifugation. in addition, the antigen was localized to the plasma membrane by electron microscopy with colloidal gold. antigen from e. histolytica strains immunoprecipitated ... | 1990 | 2307518 |
| metabolic labeling of entamoeba histolytica antigens: characterization of a 28-kda major intracellular antigen. | the in vivo incorporation of radiolabeled amino acids into antigens of entamoeba histolytica, hm-1:imss, is reported. immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kda antigen present in whole cell lysates of e. histolytica. this antigen was of cytoplasmic origin, as indicated by cell fractionation and triton x-114 detergent-phase separation. immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kda ... | 1990 | 2311709 |
| evaluation of the elisa test for detection of entamoeba histolytica in feces. | the clinical utility of an elisa test with monoclonal antibodies to detect antigen of entamoeba histolytica in feces was evaluated in 150 patients with gastrointestinal symptoms. each subject was examined by rectosigmoidoscopy with rectal smear and/or a triple stool search for ova-bacteria-parasite (obp); in addition, one stool sample was collected for the elisa test. all the tests were independent and double blind. e. histolytica was detected by obp and/or rectosigmoidoscopy in 66 patients; 61 ... | 1990 | 2313465 |
| specific labeling of cysteine proteinases in pathogenic and nonpathogenic entamoeba histolytica. | growth of entamoeba histolytica trophozoites was inhibited by 50% at low concentrations (2.0 micrograms/ml) of the diazopeptidyl inhibitor benzyloxycarbonyl-leucyl-l-tyrosyldiazomethane (z-l-leu-l-tyr-chn2). iodination of the tyrosine residue lowered the growth inhibitory efficacy of the diazopeptidyl inhibitor (50% inhibition, approximately 10 micrograms/ml). however, even at this concentration, practically all of the cysteine proteinase activity of the cells was irreversibly inactivated as sho ... | 1990 | 2323821 |
| prevalence and clinical importance of entamoeba histolytica in two high-risk groups: travelers returning from the tropics and male homosexuals. | this study was undertaken to learn the prevalence of amebiasis and to assess the clinical importance of entamoeba histolytica in two high-risk groups: 2700 travelers returning from the tropics and 320 male homosexuals. some 16.3% of the homosexual men and 4.0% of the travelers had e. histolytica infections. only five travelers showed a pathogenic zymodeme of e. histolytica, which correlated closely with invasive amebiasis with positive amebic serology and clinical symptoms with diarrhea. the hom ... | 1990 | 2324531 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 18-1990. a 58-year-old man with hematochezia and a lesion of the cecum. | | 1990 | 2325723 |
| [ulcerative colitis or amebic colitis--a case report]. | the large intestine reacts relatively monomorphically to different stimuli. from this differential-diagnostic problems may result. the history of a patient is described which could be pursued retrospectively and prospectively over 14 years and during the course of which the correction of the diagnosis ulcerous colitis into chronic relapsing amoebic colitis was necessary. as a conclusion is emphasized that in every symptomatology of colitis primarily bacterial and parasitologic examinations of th ... | 1990 | 2330735 |
| differentiation of clinical isolates of entamoeba histolytica by using specific dna probes. | most individuals infected with entamoeba histolytica are reported to be clinically asymptomatic. on the basis of the electrophoretic migration of hexokinase and phosphoglucomutase isoenzymes, two groups of e. histolytica isolates have been classified. those derived from symptomatic cases were found to have fast-migrating hexokinase bands and were labeled pathogenic. the others, isolated from cyst passers, had (in most cases) slow-migrating bands and were called nonpathogenic. differences between ... | 1990 | 2332466 |
| parasitic infections and haemoglobin level among school children of different socioeconomic classes in abha, saudi arabia. | a total of 717 school children from abha region, from upper, middle and lower social classes (182, 405 & 130 respectively), were examined for parasitic infections and haemoglobin level. giardia lamblia was found to be the most common intestinal parasite among children of all classes, entamoeba histolytica followed among children of middle class, and hymenolepis nana among those of lower one. anaemia was more prevalent among parasitically infected children particularly the multi-infected ones of ... | 1990 | 2332665 |
| repetitive dna elements characteristic of pathogenic entamoeba histolytica strains can also be detected after polymerase chain reaction in a cloned nonpathogenic strain. | strains of entamoeba histolytica which were isolated from symptomatic patients and which possess a characteristic pathogenic isoenzyme pattern (zymodeme) have extrachromosomal circular dna molecules containing rna genes and clusters of tandemly reiterated pvui elements. the nucleotide sequence of comparable reiterated bamhi elements present in amebae with nonpathogenic zymodemes differs from that found in pathogenic ones. by using the polymerase chain reaction, it was demonstrated that the clone ... | 1990 | 2341171 |
| interactions of macrophages from in vivo stimulated guineapigs & the trophozoites of entamoeba histolytica. | the interactions between peritoneal macrophages obtained from guineapigs immunized with a sephadex chromatographed fraction (fi) of crude amoebic extract proteins and trophozoites of axenic e. histolytica (nih:200) were studied in vitro in the presence or absence of anti fi antiamoebic antibodies. unstimulated macrophages killed trophozoites to some extent. incorporation of anti fi antiamoebic antibodies or igg of fi antibodies in the amoebae macrophages mixture significantly enhanced the cytoto ... | 1990 | 2345027 |
| [necrotizing amebic dysentery. a rare cause of massive colorectal hemorrhage]. | | 1990 | 2346057 |
| mechanism of tissue damage through free oxygen radicals during hepatic amoebiasis in guinea pigs. | the capacity of kupffer cells and blood monocytes to release free oxygen radicals was studied by chemiluminescence (cl) response during hepatic amebiasis in guinea pigs. guinea pigs infected with entamoeba histolytica intramesenterically were sacrificed on days 0, 2, 5 and 8 post-infection. hepatic lesions were graded i-iv. a significant increase in the cl response was observed from day 2 post-infection and it increased with the progress of infection. maximum increase was observed on the 8th pos ... | 1990 | 2347478 |
| ecological studies on intestinal microbial flora of kenyan children with diarrhoea. | the intestinal microflora was analysed together with short-chain fatty acids (scfa) and bile acids in faeces from nine children with acute diarrhoeal disease in lari, kenya. enteric pathogens such as enteroinvasive e. coli, enteropathogenic e. coli, yersinia enterocolitica, rotavirus, giardia lamblia and entamoeba histolytica were isolated either singly or in combination from diarrhoeal faecal specimens. the most striking finding in these patients was a marked reduction of anaerobes. analysis of ... | 1990 | 2348500 |
| enhancement of virulence of entamoeba histolytica by histamine in vitro. | three isolates of e. histolytica isolated and maintained in modified boeck and drbohlav's medium and the axenic nonpathogenic strain nih-200 maintained in diamond's tps-1 medium were used to assess the effect of histamine added to the cultures on their pathogenicity in just weaned rats of charles foster strain. initially when the three polyxenic isolates were examined for their pathogenicity after growing them with graded concentrations of histamine viz. m-2 through m-6 (log dilutions of the mol ... | 1990 | 2351422 |
| intestinal parasitoses in czechoslovak citizens working abroad. | this paper presents results of coprologic examinations of czechoslovak citizens in connection with their working sojourns in 50 tropical and subtropical countries in asia, africa and latin america. among 525 persons examined, pathologic findings in stool were registered in 74 persons (14.1%) during their stay abroad and after return home. the incidence of particular infections during the period under study was as follows: non-pathogenic protozoa were found in 8.2% of cases, entamoeba histolytica ... | 1990 | 2351820 |
| increase of dna content in tissue stages of entamoeba histolytica strain sfl 3. | the dna content of culture forms and tissue stages of pathogenic e. histolytica strain sfl 3 were measured photometrically after the nuclei had been stained with the fluorochrome bao. as a control, the dna guartity of e. histolytica strain hk 9 and e. invadens were determined by the same method and compared with reference values. tissue stages were obtained from hamsters experimentally infected by intrahepatic injection of sfl 3 amoebae. further studies concerning possible changes in the dna con ... | 1990 | 2352913 |
| uses and limitations in the demonstration of specific circulating immune complexes in patients with amoebiasis. | a micro-enzyme linked immunosorbent assay (micro-elisa) has been evaluated as a diagnostic test to detect amoebic antigen in polyethylene glycol (peg) precipitated circulating immune complexes (cic) in sera from patients with amoebiasis. the immune complexes were captured on rabbit anti-amoebic igg-coated wells of microtitration plates and the complexed antigen was detected by enzyme linked antihuman immunoglobulins. a titre of greater than 160 for the immune complexes was considered to be of cl ... | 1990 | 2355392 |
| immune response in patients with amoebiasis: evaluation of igg-subclasses. | in order to evaluate the immune response with respect to igg-subclasses (igg1-igg4) in patients with extraintestinal amoebiasis, an elisa technique was established. it was the aim of this pilot study to quantify the igg subclass response and to compare the resulting pattern with other systemic protozoal infections. our results give evidence that igg4 contributes to more than one third of the total immune response, followed by igg2, igg3 and igg1. regarding the igg4 response in patients with plas ... | 1990 | 2360971 |
| parasitic infections in hiv patients in austria: first results of a long-term study. | in the course of a long-term study of parasitic infections among hiv-infected persons in austria during the period from november 1985 until may 1989, 618 persons infected with hiv (including 270 hospitalized patients, most of them with severe symptoms of aids) were examined. 58% of all persons had antibodies against toxoplasma gondii. the incidence of clinically overt toxoplasmosis was about 20% in the 167 hospitalized persons infected with the parasite. in 29% of 68 patients with suspected pneu ... | 1990 | 2360972 |
| [taeniasis, amebiasis and other intestinal parasitosis in school age children from michoacan state, mexico]. | 92.3% schoolchildren aged 6-13 years of a mexican rural village, suspected foci of taenia solium cysticercosis were screened for intestinal parasites with the main purpose to know the infection rate by taeniasis. an stool sample was collected to schoolchildren of the village and 95.4% of a urban private school as comparative group. laboratory examinations were performed with the most accurate technics, included microscopies with an ocular micrometer. the general parasitation rate was 4 times hig ... | 1990 | 2360984 |
| intestinal parasites in refugee claimants: a case study for selective screening? | the medical profiles of 1,967 refugee claimants to montreal, quebec, canada from january 1987 to july 1987 were reviewed to evaluate the importance of imported intestinal parasite infection in this group and to re-examine the screening policy governing these infections. an overall infection rate of 29.3% was obtained for pathogenic parasites, where helminths were four times more frequently found than the protozoa entamoeba histolytica and giardia lamblia. age, sex, years of schooling, country of ... | 1990 | 2361204 |
| non-pathogenic entamoeba histolytica: functional and biochemical characterization of a monoxenic strain. | we have cultured under monoxenic conditions and characterized an entamoeba histolytica clone, mav-i cinvestav (mav-i), obtained from feces from an asymptomatic carrier. the clone shows the non-pathogenic e. histolytica zymodeme type i, which did not change through the process of monoxenization. clone mav-i was non-pathogenic in both in vivo and in vitro tests, and it did not have a functional 112-kda adhesin. as far as we know, this is the first non-pathogenic monoxenic strain reported. clone a ... | 1990 | 2362603 |
| amoebiasis in foreign students. | a total of 2,883 foreign students at the age of 18-30 years were examined for amoebiasis after their arrival to czechoslovakia. stool examinations revealed the presence of entamoeba histolytica in 112 of them (3.9%). students from 38 countries were found to be infected with this parasite. in a set of 2,064 students from these countries e. histolytica prevalence in stool was 5.4%. there were greater differences in the prevalence between individual countries inside a geographical region than betwe ... | 1990 | 2370032 |
| entamoeba histolytica: physiology of multidrug resistance. | cross-resistance to unrelated drugs has been previously observed in multidrug-resistant carcinoma cells and the goal of this work was to determine whether a similar mechanism existed in entamoeba histolytica. an emetine and a colchicine-resistant clone, c2(90) (ic50 = 62 microm, and 1.5 mm, respectively), and the parental clone, a (ic50 = 5 microm and 1 mm, respectively), were analyzed for resistance to other drugs and for the effect of verapamil. both clones, c2(90) and a, exhibited similar res ... | 1990 | 2373187 |
| entamoeba histolytica: correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase. | work from several laboratories suggests a correlation between expression of cysteine proteinase activity and the cytopathic effect of virulent hm1 strain entamoeba histolytica trophozoites on cultured cell monolayers. consistent with this relationship, we find that l-6 trophozoites, mutants cloned from the hm1 parent strain, are deficient in both proteinase expression and cytopathic effect. three other clones, with proteinase expression equal to or greater than that of the hm1 strain, express th ... | 1990 | 2373188 |
| [diarrhea cause by parasites]. | parasites, once an exclusively tropical problem, are rare causes of diarrhea in our countries. increasing tourism and immune deficiencies due to treatment or to aids lead to an increase of intestinal parasites in western countries. the most frequent pathogenic parasites are giardia lamblia and entamoeba histolytica but other organisms like cryptosporidia seem to be of increasing importance and have to be considered in diarrhea of unknown origin. | 1990 | 2374858 |
| [a fatal case of amebic dysentery]. | the paper describes a case of complicated amebic dysentery imported from india in a 39-year old man. even in microscopic examination of feces, amebiasis diagnosis presented difficulties for clinicists. | 1990 | 2377143 |
| parasite detection efficiencies of five stool concentration systems. | fresh fecal material that was free of ova and parasites was pooled with 10% formalin in a 1:4 ratio to prepare a standard specimen. portions of 100 ml of this specimen were individually seeded with cryptosporidium oocysts, entamoeba coli, entamoeba histolytica, and giardia lamblia cysts; ova of necator americanus; and strongyloides larvae. appropriate volumes of each parasite suspension were used to evaluate the fecal concentrator kit (remel, lenexa, kans.), fecal parasite concentrator (evergree ... | 1990 | 2380347 |
| investigation of seemingly pathogen-negative diarrhoea in patients infected with hiv1. | thirty three consecutive patients infected by human immunodeficiency virus type 1 (hiv1) with persistent diarrhoea which remained undiagnosed after microbiological examination of six stool samples and rectal histology were investigated for malabsorption. all had xylose and schilling tests, distal duodenal biopsy, comprehensive barium studies, microbiological examination of six further stool samples, and repeat rectal histology. a microbiological or histological diagnosis of infection was made in ... | 1990 | 2387512 |
| association of serum antibodies to adherence lectin with invasive amebiasis and asymptomatic infection with pathogenic entamoeba histolytica. | the recognition of the entamoeba histolytica galactose-inhibitable adherence lectin by antibodies was studied using sera obtained from subjects in south africa with an amebic liver abscess or asymptomatically pathogenic or nonpathogenic e. histolytica infection and from uninfected regional controls. in addition, sera from healthy american controls or americans known to be infected with other parasites were studied. of the 95 sera containing antibodies to total parasite protein, 95% demonstrated ... | 1990 | 2388003 |
| high seropositivity for entamoeba histolytica infection in japanese homosexual men: further evidence for the occurrence of pathogenic strains. | | 1990 | 2389318 |
| in vitro effect of human recombinant tumor necrosis factor on entamoeba histolytica trophozoites. | the effect of tumor necrosis factor (tnf) on e. histolytica trophozoites was examined by using three virulent (ip: 0682:1, hm-1: imss, 200: nih) and one nonvirulent (dkb) strain of e. histolytica. various concentrations of recombinant tnf were added to e. histolytica trophozoites and the total parasite numbers and their viability were periodically assessed by microscopic observation and trypan blue staining after incubation at 37 degrees c in a nonhumidified chamber. in this study, concentration ... | 1990 | 2397931 |
| amplification and sequencing of genomic dna fragments encoding cysteine proteases from protozoan parasites. | cysteine protease gene fragments from three protozoan parasites trypanosoma cruzi, trypanosoma brucei, and entamoeba histolytica were amplified by the polymerase chain reaction (pcr) from genomic dna using degenerate oligonucleotide primers. the primers used for the amplification were designed based upon amino acid sequences flanking the active site cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteases analyzed to date. the amplified dna fragments, representing ... | 1990 | 2406590 |
| epidemiology of travelers' diarrhea and relative importance of various pathogens. | each year 12 million persons travel from an industrialized country to a developing country in the tropics or subtropics. these travelers experience a high rate of diarrhea caused by a wide variety of enteric pathogens acquired by ingestion of contaminated food or water. one or more pathogens can be found in the stool of a majority of ill individuals. enterotoxigenic escherichia coli generally are the most frequently identified pathogens, having been found in a median of 42% of travelers' diarrhe ... | 1990 | 2406860 |
| the biochemistry and functional morphology of the entamoeba. | | 1985 | 2409275 |
| routine procedures for examination of stool and blood for parasites. | the diagnosis of a parasitic infection depends upon demonstration of the organism in stool, blood, or tissue specimens, and a variety of relatively simple procedures is available for this purpose. in this article, the author describes those procedures that are most frequently used on a routine basis. | 1985 | 2410850 |
| [translation of rna obtained from entamoeba histolytica and its characterization of a specific monoclonal antibody]. | | 1985 | 2413554 |
| histopathological diagnostics of liver amoebiasis in cambodia. | results of histopathological examination of a solitary chronical amoebic abscess in the liver of a patient from cambodia are described. the abscess was at a proliferously reparative phase and entamoeba histolytica was detected at the border between the necrosis and inner wall of abscess, as well as in the proper non-specific granulation tissue of the inner layers of abscess capsule. a differential diagnosis of the amoebic abscess from similar parasitary or pseudoparasitary liver lesions and diff ... | 1985 | 2417921 |
| antibody response of patients harbouring different zymodemes of entamoeba histolytica to homologous and heterologous antigens. | entamoeba histolytica isolates characterized into four zymodemes were used as antigens in the ifat; with 17 sera obtained from patients harbouring different amoebic isoenzyme groups. the method of cultivation used was found to influence the ease of reading the ifat. reactivity of serum samples, obtained from the selected patients (i.e. with varied forms of amoebiasis), against homologous and heterologous antigens did not reveal any antigenic differences among amoebic zymodemes. the present resul ... | 1985 | 2418210 |
| immune consequences of amebiasis. | | 1986 | 2418425 |
| lethal recognition between entamoeba histolytica and the host tissues. | lethal recognition between entamoeba histolytica and the tissues and defence systems of the host results in a continuous interplay that determines the development of pathological lesions: (i) we have identified several of the steps and mediators utilized by the trophozoites to destroy host cells by contact-mediated cytolysis; (ii) we have established that the alternative complement system represents the main defence available to the host against the invading parasite. the amoebae recognize targe ... | 1985 | 2420045 |
| studies on the substrate specificity of alpha- and beta-amylase of entamoeba histolytica. | from the supernatant fraction of cell homogenates of entamoeba histolytica alpha-amylase (1,4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1) and beta-amylase (1,4-alpha-d-glucan maltohydrolase, ec 3.2.1.2) were separated and purified by gel filtration and isoelectric focusing, followed by deae- and cm-chromatography, respectively. both enzymes catalyzed the degradation of amylose, amylopectin and glycogen. hydrolysis of polysaccharides by alpha-amylase yielded as reaction products maltose, maltotr ... | 1986 | 2421162 |
| separation and characterization of four different amylases of entamoeba histolytica. i. purification and properties. | cell homogenate of entamoeba histolytica trophozoites was investigated for amylolytic activity against various biogenic and synthetic substrates. after gel filtration of the cell homogenate on sephadex g-150, six partly separated amylases (i to vi) differing in their substrate specificities were detected using maltose, amylose, amylopectin, 4-nitrophenyl alpha-glucoside and 4-nitrophenyl alpha-maltotetraoside. all enzymes are able to degrade amylose, amylopectin, glycogen and biogenic malto-olig ... | 1986 | 2423097 |
| separation and characterization of four different amylases of entamoeba histolytica. ii. characterization of amylases. | purified e. histolytica amylases iii to vi were characterized by their hydrolytic behaviour towards 4-nitrophenyl alpha-malto-oligosaccharides, malto-oligosaccharides, amylose, amylopectin, glycogen and y-cyclodextrin. the influence of specific inhibitors on the amylase activity of e. histolytica was examined and compared with typical alpha- and beta-amylases. amylases iii and iv showed alpha-glucosidase and glucosyltransferase activity by cleaving terminal non-reducing glucose from pnpg1 (iii, ... | 1986 | 2423098 |
| entamoeba histolytica: specific antigen recognized by a monoclonal antibody. | specific antigenic determinants on the membrane surface of entamoeba histolytica that distinguish it from other entamoeba species were demonstrated. evidence for these antigenic determinants was obtained with a monoclonal antibody to e. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of e. histolytica trop ... | 1986 | 2423362 |
| assessment of some preservatives and stains for entamoeba histolytica. | | 1986 | 2431077 |
| acid intracellular vesicles and the cytolysis of mammalian target cells by entamoeba histolytica trophozoites. | entamoeba histolytica kills mammalian target cells in a multi-step sequential process with separate adherence, cytolytic, and phagocytic events. in the studies reported here, we used fluorescein isothiocyanate linked to dextran to label the endocytic vesicles of the hm1 strain of e. histolytica and measure vesicle ph (5.1 +/- 0.2 by spectrofluorimetry). concentrations of nh4cl (1.0-10.0 mm) sufficient to increase vesicle ph to greater than or equal to 5.7 inhibited amebic killing of target chine ... | 1986 | 2432267 |
| the interaction of human t-lymphocytes and entamoeba histolytica: killing of virulent amoebae by lectin-dependent lymphocytes. | clinical and experimental studies indicate that following invasive disease due to entamoeba histolytica, development of human cell-mediated immune mechanisms may provide protective immunity. activated, human monocyte-derived macrophages in vitro can kill virulent axaenic amoebic trophozoites. this study describes the interaction of lectin-stimulated t-lymphocytes and e. histolytica trophozoites (virulent strain hm1-imss). amoebae progressively killed unstimulated nonimmune t-lymphocytes over 18 ... | 1987 | 2437517 |