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aspertoxin, a hydroxy derivative of o-methylsterigmatocystin from aflatoxin-producing cultures of aspergillus flavus. 19685651008
investigation of reported aflatoxin production by fungi outside the aspergillus flavus group.a screening study of 121 fungus isolates, representing 29 species, for aflatoxin synthesis demonstrated this property only in aspergillus flavus and a. parasiticus. eight of the organisms found negative were isolates reported by other investigators to produce aflatoxin. since similar negative reports have come from several other workers, it is concluded that only the a. flavus group of aspergillus can presently be certified as sources of these toxins. reasons for possible false-positive findings ...19685664105
[urate oxidase of aspergillus flavus. i. isolation, purification, properties]. 19685671592
[urate oxidase of aspergillus flavus. ii. metabolism, inhibition, specificity]. 19685671593
the autolysis of aspergillus flavus in an alkaline medium. 19685677976
afltoxin formation by aspergillus flavus and aspergillus parasiticus in a casein substrate at different ph values. 19685685588
[study of the biological properties of aflatoxin of aspergillus flavus fungi isolated from peanut chaff and shell]. 19685690767
luminescent study of aspergillus flavus link strains which release beta-indolylacetic acid. 19685725797
enrichment of auxotrophic mutants of aspergillus flavus by tritium suicide.a method based on tritium suicide was developed to enrich auxotrophic mutants of aspergillus flavus. n-methyl-n'-nitro-n-nitrosoguanidine (ng) was chosen as a mutagen, since a wide variety of mutations were induced by the action of 0.1% ng on a. flavus conidia suspended in phosphate buffer (ph 7.0). the decimal reduction time under these conditions was about 30 min, and the surviving population contained 4 to 6% auxotrophs after 1 hr of mutagenesis. this proportion was then increased by tritium ...19685726297
detecting farmers' stock peanuts containing aflatoxin by examination for visible growth of aspergillus flavus. 19695768610
isolation and characterization of alkaline proteinase of aspergillus flavus. 19695773446
aflatoxin produced by 1,626 isolates of aspergillus flavus from groundnut kernels and soils in israel. 19695784436
influence of refrigeration on aflatoxin production by strains of aspergillus flavus. 19695798520
coconut as a medium for the experimental production of aflatoxin.fresh, grated coconut has been found to be an excellent medium for aflatoxin production by aspergillus flavus. under optimal conditions, yields of 8 mg of total aflatoxin per g of substrate were obtained. continuous agitation of the growth medium under moist conditions at 24 c produced highest yields. aflatoxin was assayed both biologically and chromatographically. the aflatoxin content of cultures varied biphasically with the duration of incubation. it is suggested that this pattern could resul ...19695803632
[chemical and physiological changes in filamentous fungi during autolysis. vi. variation in the amounts of ammonia evolved during induced autolysis in aspergillus flavus]. 19695811082
pathogenicity of aspergillus flavus link to musca domestica nebulo fabricius. 19655848798
field observations on conditions conducive to the contamination of groundnuts with the mould. aspergillus flavus. link ex fries. 19655853117
variations inth the cultural characteristics of rhizoctonia solani, and its antagonists: aspergillus terreus and aspergillus flavus occurring in the rhizosphere of cotton. 19655877017
role of 3-nitropropanoic acid in nitrate formation by aspergillus flavus.doxtader, k. g. (cornell university, ithaca, n.y.), and m. alexander. role of 3-nitropropanoic acid in nitrate formation by aspergillus flavus. j. bacteriol. 91:1186-1191. 1966.-aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-npa), kojic acid, and a substance tentatively identified as n-formyl-n-hydroxy-glycine during growth in a medium with ammonium as sole nitrogen source. the concentration of the nitro compound reached a maximum prior to the appearance of nitrate; the 3-npa level ...19665929750
spicaria rileyi (farlow) charles and aspergillus flavus link, entomogenous fungi of the cabbage looper, trichoplusia ni (hübner), in indiana and wisconsin. 19665948338
toxic factors of mould origin.the chemistry and effects of mycotoxins associated with human and animal foodstuffs are reviewed. the aflatoxins, metabolites of aspergillus flavus, have been implicated in fatal diseases of farm stock fed on infected peanut cake. muscarine and the phalloidins are the causative agents in mushroom poisoning. lysergic acid alkaloids are involved in ergotism. "yellow rice" toxicity arises from infection with penicillium islandicum, the active principles being islanditoxin and luteoskyrin. various s ...19665948367
biotin and fatty acid biogenesis in aspergillus flavus. 19665964093
[the toxicity of malt seeds containing aspergillus flavus]. 19665966800
aflatoxin production and degradation by aspergillus flavus in 20-liter fermentors.yields of from 200 to 300 mg per liter of aflatoxins b(1) and g(1) were produced by two strains of aspergillus flavus in 20-liter fermentors under proper conditions of inoculum (well-dispersed growth) and aeration (0.5 volume per volume per min of air, 300 rev/min, 30 psi back pressure, baffles). peak yields were usually attained in 72 hr, after which the aflatoxin concentration declined rapidly. degradation of aflatoxin depended primarily on mycelial lysis and high-aeration conditions. cultures ...19665970470
production of aflatoxins b1 and g1 by aspergillus flavus in a semisynthetic medium.isolates of aspergillus flavus produced 0.2 to 63 mg of aflatoxins b(1) and g(1) per 100 ml in a nutrient solution consisting of 20% sucrose and 2% yeast extract. various factors influencing the fermentation were studied. the maximal amount of toxin was produced by atcc culture 15548 in 1-liter flasks containing 100 ml of medium incubated as stationary cultures for 6 days at 25 c.19665970823
production of aflatoxin on rice.a method has been developed for the production of aflatoxin by growing aspergillus flavus strain nrrl 2999 on the solid substrate rice. optimal yields, more than 1 mg of aflatoxin b(1) per g of starting material, were obtained in 5 days at 28 c. a crude product containing aflatoxins was isolated by chloroform extraction and precipitation with hexane from concentrated solutions. the crude product consisted of 50% aflatoxin in the following ratio: b(1)-b(2)-g(1)-g(2), 100:0.15:0.22:0.02. aflatoxin ...19665970829
aflatoxin production by isolates of aspergillus flavus. 19665980345
effect of glucose on the oxidation of beta-nitropropionic acid by aspergillus flavus.the rate of oxidation of beta-nitropropionic acid (bnp) to nitrate by replacement cultures of aspergillus flavus was linear. for each micromole of bnp assimilated, 1 mumole of nitrate was detected. bnp was readily oxidized at ph 3.8, but not at ph 6.8. in the presence of glucose, nitrate was not detected until after all of the glucose had been utilized. the effect of glucose is attributed to its inhibition of bnp uptake and also to its role as an electron donor for a nitrate reductase.19676027999
aflatoxin-producing potential of isolates of the aspergillus flavus-oryzae group from peanuts (arachis hypogaea).seventy-eight samples of farmer stock peanuts, representing peanuts grown in nine different geographical areas during 1964, were assayed for aflatoxin and examined for associated microflora. only two samples contained more than 50 ppb of aflatoxin. infestation by members of the aspergillus flavus-oryzae group varied from 35 to 100% of the kernels per area and from 1 to 100% of the kernels per sample. aflatoxin production by individual isolates ranged from 0 to 349,143 ppb under the test conditio ...19676031427
production of aflatoxin on wheat and oats: measurement with a recording densitometer.a method has been developed for the production of aflatoxin by growing aspergillus flavus nrrl 3145 on solid substrate wheat. optimal yields of 900 mug of aflatoxin g(1) and 900 mug of aflatoxin b(1) per g of substrate were obtained in 4 to 5 days at 28 c. a study of aflatoxin production on hulls and groats of oats and on whole oats by a. flavus strains nrrl 2999, nrrl 3000, and nrrl 3145 revealed that aflatoxin was produced on all three substrates, although production was very slight on hulls. ...19676031432
microflora of black and red pepper.dilution cultures of 30 samples of ground black pepper yielded an average of 39,000 colonies of fungi per g, with a range of 1,700 to 310,000 per g. total numbers of colonies of bacteria from 11 samples averaged 194,000,000 per g, with a range from 8,300,000 to 704,000,000 per g. a variety of fungi grew from nearly all surface-disinfected whole peppercorns that were cultured. thirteen samples of ground red pepper from the united states yielded an average of 1,600 colonies of storage fungi per g ...19676035055
limiting temperature and relative humidity for growth and production of aflatoxin and free fatty acids by aspergillus flavus in sterile peanuts. 19676041385
experimental study of the pathogenicity of aspergilli for mice.the relative virulence was determined for 14 species of aspergilli, by inoculating normal mice intravenously with graded doses of spores. eleven were found to possess some degree of virulence, whereas three others were avirulent. members of the aspergillus flavus group were the only species that consistently killed mice with doses as low as 10(4) viable spores. when the in vivo fate of spores was compared for a virulent and an avirulent strain of aspergillus, spores of the latter were cleared ra ...19676051365
formation of aflatoxin in cheddar cheese by aspergillus flavus and aspergillus parasiticus. 19676059382
influence of atmospheric gases on aflatoxin production by aspergillus flavus in peanuts. 19676075677
[studies on the pathogenic effects of aspergillus flavus on geese]. 19676076546
in vitro susceptibility studies with oxiconazole (ro 13-8996).oxiconazole (ro 13-8996) is a recently described imidazole derivative intended for topical use. 128 isolates of pathogenic fungi were tested in vitro against oxiconazole, miconazole, and ketoconazole using an agar dilution method. results indicated that miconazole was markedly more active than either oxiconazole or ketoconazole against candida albicans while ketoconazole was the more active compound against candida parapsilosis. a species specific difference in the susceptibilities of isolates o ...19846086246
in vitro studies with r 51,211 (itraconazole).the in vitro activity of r 51,211 (itraconazole, accepted generic name; janssen pharmaceutica, beerse, belgium), a new orally active triazole, was compared with those of two existing orally active azoles, ketoconazole and bay n 7133, and a topical agent, ro 14-4767/002. an agar dilution procedure (kimmig agar) was performed with 148 isolates of pathogenic fungi. incubation was at 30 degrees c from 48 h to 7 days. r 51,211 was dissolved in 0.2 n hcl in absolute ethanol, ketoconazole was dissolved ...19846089654
the preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several aspergillus species.extracts with immunological activity were prepared from aspergillus fumigatus, a. flavus, a. terreus, a. niger and a. nidulans. in each case crude mycelial wall was extracted with an aqueous solution of triton x-100 giving detergent-soluble material. further fractionation was achieved by removing the detergent from this solution; the resultant precipitate was removed by centrifugation, and the aqueous supernatant was used as a source of soluble antigens. the sensitivity of these preparations was ...19806157778
immunoperoxidase staining in the recognition of aspergillus infections.this study concerns the use of the indirect immunoperoxidase technique for labelling aspergillus species in infected human and animal tissues in smears taken from cultures. specific labelling without serious cross-reactivity with candida species or zygomycete fungi was demonstrated using antiserum to aspergillus. however, it was not possible to distinguish between two aspergillus species. a fumigatus and a. flavus with this technique. pretreatment of sections with sodium azide and normal swine s ...19816168556
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...19836195179
aspergillus infection of a permanent ventricular pacing lead.fungal infections of intracardiac pacemakers are extremely rare. we describe what appears to be the first reported case of aspergillus infection of a permanent pacing lead and review the three reported cases of other fungal infections of pacemaker leads which we could find in the literature. these infections may be encountered more frequently as the number of permanent pacemakers implanted in elderly patients with underlying medical illnesses continues to increase.19846204287
specificity of antigens from pathogenic aspergillus species. ii. studies with line immunoelectrophoresis.somatic (mycelial) and metabolic (culture filtrate) antigens of aspergillus flavus, a. fumigatus, a. nidulans, a. niger and a. terreus were compared by line immunoelectrophoresis with sera from patients with allergic bronchopulmonary aspergillosis (abpa) or aspergilloma, or from immunized animals. number of lines observed when tested with human sera were similar for somatic and metabolic preparations of a. fumigatus, but up to 33 lines were present when both types of antigens were tested simulta ...19846209814
nickel toxicity to fungi: influence of environmental factors.the toxicity of nickel (ni) to the mycelial growth rates of filamentous fungi was influenced by environmental abiotic factors. increasing the ph from acidic to alkaline levels completely eliminated the toxicity of ni to achyla sp. and saprolegnia sp. magnesium or zinc, but not potassium, sodium, calcium, or ferric, ions reduced the toxicity of ni to achyla sp. an antagonistic interaction between a combination of ni + pb was noted toward growth of achyla sp. and saprolegnia sp.; the interactions ...19826220888
[contamination of various food products with aflatoxins in the dzhambul district of the kazakh s.s.r]. 19836222540
[aflatoxin content of vegetable foods in the georgian s.s.r]. 19836222541
avian pox infection, aspergillosis and renal trematodiasis in a royal tern.poxvirus infection and aspergillosis were diagnosed in a royal tern (thalasseus maximus) based on gross and microscopic lesions. this represents the first known report of avian pox in a tern. renal trematodiasis, caused by a species of renicola also is described.19806257938
ultrastructural changes in gamma-irradiated aspergillus flavus spores.ultrastructural changes in gamma-irradiated aspergillus flavus were investigated. electron microscopic studies revealed lipid-like inclusions, aggregated in the spore lumen near the cell membrane in the radiation-induced mutants. their absence in the parent strain may indicate their direct significance in aflatoxin production.19816269807
ketoconazole: a new imidazole antifungal agent has both prophylactic potential and therapeutic efficacy in keratomycosis of rabbits.a highly reproducible quantitative model of fungal infection of the rabbit's corneal stromal was produced using multiple corneal microtrephination. aspergillus flavus (k4/77), at a concentration of 10(6) spores per ml was systematically implanted into the trephine sites in the cornea, and the degree of corneal infection determined. by pre-inoculation and post-inoculation challenge of these cornea with 1% ketoconazole in arachis oil, the prophylactic potential and the therapeutic usefulness of ke ...19826295973
rapid, economical qualitative method for separation of aflatoxins b-1, b-2 & g-1, g-2 by dry column chromatography.a good correlation of four components of aflatoxins was accomplished by using the dry column chromatography method. the decolorization process of interfering substances, by 0.01 n koh and defatting the extract with petroleum ether yields a clean residue for dcc separation. it is clear that the dry column chromatography is a very simple and time-saving procedure for separation of aflatoxins. dcc columns are more economical than precoated 'thick layer' preparative plates and, in dcc, no large deve ...19836323985
in vitro activities of amphotericin b in combination with four antifungal agents and rifampin against aspergillus spp.strains of aspergillus fumigatus, aspergillus flavus, and aspergillus niger were tested for in vitro susceptibility with a microtiter plate system in buffered yeast-nitrogen base and in buffered minimal essential medium. isolates were tested against amphotericin b, flucytosine, rifampin, ketoconazole, ici 153,066, and bay n 7133 and against combinations of amphotericin b with each of the other five drugs. combinations of amphotericin b and rifampin were the most active against all three species ...19846329088
aspergillosis of the cerebral ventricles in a heroin abuser. case report and review of the literature.aspergillosis of the cerebral ventricles developed in a 36-year-old heroin abuser who died during the sixth week of illness. the diagnosis was not made during life. a review of our case and of four previous cases from the literature indicates that aspergillosis should be considered in parenteral drug abusers with the syndrome of chronic meningitis or ventriculitis. the diagnosis is difficult to establish, but serologic tests may be helpful. this form of cns aspergillosis can be cured with antifu ...19836401419
aspergillus epiglottitis.a 21-year-old woman with acute lymphocytic leukemia developed clinical and radiographic signs of epiglottitis. premortem and postmortem histologic studies showed invasive aspergillosis; aspergillus flavus was grown in culture. the necrotizing nature of this infection is explained by the predilection of aspergillus sp. for invasion of blood vessels. the usual causative agents of epiglottitis are bacteria; the association with fungal infection has not been previously described.19836401587
primary aspergillosis. 19836402611
effects of soil moisture and temperature on preharvest invasion of peanuts by the aspergillus flavus group and subsequent aflatoxin development.four soil temperature and moisture treatment regimens were imposed on florunner peanuts 94 days after planting in experimental plots in 1980. at harvest (145 days after planting), the incidence of the aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. extensive colonization of sound mature kernels (smk) by the a. flavus group occurred with the drought stress treatment (56% kernels colonized); colonization was less in the irrigated plot (7%) and the drought ...19836402980
intrafungal distribution of aflatoxins among conidia and sclerotia of aspergillus flavus and aspergillus parasiticus.this research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of aspergillus flavus link and aspergillus parasiticus speare cultured on czapek agar (21 days, 28 degrees c). total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among strains. aspergillus flavus nrrl 6554 accumulated the highest levels of aflatoxin (conidia: b1, 84000 ppb; g1, 566000 ppb; sclerotia: b1, 135000 ppb; g1, 968000 ppb). substantial afl ...19836403210
toxicity of fungal metabolites to albino rats.the oral intubation of culture filtrates produced nephrotoxic damages in the form of marked capillaritis, shrinkage and early fibrosis of glomeruli. hypertrophy and degeneration of tubular epithelium were also evident. the spleen of treated animals revealed subacute splenitis in the form of congestion of the splenic sinuses.19836404008
aspergillomycosis in intensively cultured tilapia from kenya. 19836404044
influence of benzoflavone on aflatoxin b1-induced cytotoxicity, mutation, and transformation of c3h/10t1/2 cells.aflatoxin b1 (aflb1), a metabolite of the fungus aspergillus flavus, is hepatotoxic and hepatocarcinogenic in several animal species and is thought to play an etiological role in human liver cancer. c3h/10t1/2 clone 8 mouse embryo fibroblasts are killed, mutated, and morphologically transformed byaflb1. 7,8-benzoflavone, a known inhibitor of aryl hydrocarbon hydroxylase, inhibits this enzymatic activity in c3h/10t1/2 cells. furthermore, benzoflavone inhibits the binding of aflb1, to the dna of c ...19836406051
an improved medium for the detection of aspergillus flavus and a. parasiticus.an effective selective medium for the enumeration of aspergillus flavus and aspergillus parasiticus has been developed by modification of bothast and fennell's aspergillus differential medium. results can be obtained with the new medium, aspergillus flavus and parasiticus agar (afpa), after 42 h incubation at 30 degrees c. the medium is thus suitable for use in quality control as a guide to the presence of a. flavus and, potentially, of aflatoxins. afpa has been extensively tested on peanuts and ...19836406419
effect of dietary aflatoxins on protein and energy utilization by two indonesian breeds of ducklings (alabio and tegal). 19836408629
aspergillosis of the paranasal sinuses. 19836409580
[aflatoxin m1 - isolation of a toxin-producing strain of the mold aspergillus flavus].a strain of aspergillus flavus producing aflatoxins b1, g1 and m1 was obtained. aflatoxin m1 constitutes about 5% of the total amount of aflatoxins produced. aflatoxin m1 was isolated from a crude chloroform extract and was identified by thin silica-gel layer chromatography (tlc), by high-performance liquid chromatography (hplc) and spectral analysis.19836412419
mycologic identification of emericella nidulans and aspergillus flavus causing pulmonary infection. 19836413149
effect of contamination of diets with aflatoxins on growing ducks and chickens.growing alabio ducks and white leghorn chickens were used in a growth study in which diets containing either soybean meal (sbm), peanut meal (pnm) or fish meal (fm) as protein sources were contaminated with the fungus aspergillus flavus providing the following aflatoxin levels: 0, 50, 100 and 200 micrograms aflatoxin b1 equivalent per kg ration. there were no differences in responses of growing ducks and chickens (at age of 28 days) to the various protein sources at the zero aflatoxin level. how ...19836414136
production of aflatoxin by an aspergillus flavus isolate cultured under a limited oxygen supply.in a previous experiment on the preservation of hay of high moisture content with formic acid, among other agents, aflatoxin was formed in the hay, and aflatoxin-forming strains of aspergillus flavus were isolated from this hay after incubation in air as well as in an anaerobic jar. one isolate from the anaerobic jar was cultivated in a chemostat (bioflo model c 30; new brunswick scientific co.) in a defined medium with added b vitamins, yeast extract, or formic acid, with or without gas flow (a ...19836414371
effect of pyridazinone herbicides on growth and aflatoxin release by aspergillus flavus and aspergillus parasiticus.the influence of pyridazinone herbicides on aflatoxin production by aspergillus flavus and a. parasiticus was studied in liquid media. mycelia production was not affected by 20, 40, or 60 micrograms of herbicide per ml; however, aflatoxin production by a. parasiticus was higher in media with herbicide, whereas a. flavus produced lower aflatoxin levels.19836414373
[aspergillus infection of severe burns].aspergillus, which has a saprophytic life, is an opportunistic fungus and manifests his pathogenicity when a weakened state of the host facilitates its development. the conditions are particularly favorable in severely burned patients like the 54-year-old woman presented here. the criteria of pathogenicity of this opportunistic fungus, the incidence of epidemiology and prognosis of this type of infection and the clinical signs that allow an early diagnosis are discussed here.19836414854
stimulation of aflatoxin biosynthesis by lipophilic epoxides.epoxy fatty acids added to the culture media either with the inoculum or at the end of exponential growth phase stimulated aflatoxin production by toxigenic strains of aspergillus flavus and aspergillus parasiticus. this effect did not appear when the unsaturated fatty acids used for the synthesis of the epoxides and the polyhydroxyacids (which can be considered to be derived from the opening of the oxirane ring) replaced the epoxides in the culture media. no significant differences were detecte ...19836415222
osmophilism in foodgrain storage fungi.a comparative study of different species of storage fungi isolated from rice showed them to be truly osmophilic rather than osmotolerant. their osmophilism was of a high order, their optimum sucrose requirement being in the range of 50% to 75% w/v in the medium. however, only aspergillus glaucus was obligately osmophilic. a sucrose concentration of 50% w/v in czapek-dox agar was concluded to be the most suitable for the isolation of these different fungi from foodgrains.19836415482
cyclopiazonic acid production by aspergillus flavus and its effects on broiler chickens.cyclopiazonic acid (cpa) was purified from cultures of aspergillus flavus, and ca. 14 g of the toxin was collected for use in feeding studies. chicken rations were artificially contaminated with purified cpa at concentrations of 10, 50, and 100 ppm (microgram/g) and fed ad libitum to eight groups of chickens for 7 weeks. chickens receiving feed with 100 ppm of cpa had high mortality, decreased weight gain, and poor feed conversion when compared with birds receiving other doses. postmortem examin ...19836416167
effect of specific amino acids on growth and aflatoxin production by aspergillus parasiticus and aspergillus flavus in defined media.four amino acids were used as sole nitrogen sources or as supplements to ammonium sulfate, and casein and ammonium sulfate were used as sole nitrogen sources to examine their effects on aflatoxin production by aspergillus parasiticus nrrl 2999 and aspergillus flavus 3357 grown on synthetic liquid media. in general, when proline, asparagine, casein, and ammonium sulfate were used as sole nitrogen sources, they supported more growth and toxin production than tryptophan or methionine. however, prol ...19836416168
aspergillosis in a red-crowned crane. 19836417091
an atypical aspergillus flavus from a case of bronchopulmonary aspergilloma. a contribution to the cultural and serological diagnosis of a. flavus infections.a slow-growing, non-pigmented hyphomycete was isolated from sputum and a fungus ball removed at autopsy from the lung. the patient, an alcoholic, also suffered from cirrhosis and primary carcinoma of the liver. the atypical and otherwise non-recognizable isolate showed a growth typical of aspergillus flavus when cultured on sterilized peanuts. this observation indicates the diagnostic significance of such strains which have become atypical, as etiologic agents and sources of antigen. serological ...19836417947
aspergillus endophthalmitis in an intravenous drug user.a 27-year-old male drug user administered methamphetamine hydrochloride solution intravenously (iv) from a storage vial. within two weeks he was admitted with a fungal endophthalmitis of the right eye. a diagnostic and therapeutic vitrectomy was performed. aspergillus fumigatus was cultured from the drug storage vial and the vitreous biopsy specimen. the endophthalmitis was not controlled by iv and intravitreal amphotericin b. pathological examination revealed fungal abscesses in the vitreous an ...19836418052
rapid hematogenous dissemination of aspergillus fumigatus and a. flavus spores in turkey poults following aerosol exposure.cultures of blood samples taken from 3-week-old turkey poults exposed to aerosolized spores of either aspergillus fumigatus or a. flavus for 15 min yielded organisms. aspergillus fumigatus was also isolated from brain and liver tissue samples taken immediately after exposure. exfoliated cells from the lungs of 10 poults exposed to aerosolized spores of a. fumigatus were allowed to attach to glass slides in tissue culture. several types of the fixed and stained cells had attached or ingested spor ...19836418131
factors influencing the inhibition of aflatoxin production in corn by aspergillus niger.aspergillus niger, a mold commonly associated with aspergillus flavus in damaged corn, interferes with the production of aflatoxin when grown with a. flavus on autoclaved corn. the ph of corn-meal disks was adjusted using naoh-hcl, citric acid-sodium citrate, or a water extract of a. niger fermented corn. aflatoxin formation was completely inhibited below ph 2.8-3.0, irrespective of the system used for ph adjustment. when grown in association with a. flavus nrrl 6432 on autoclaved corn kernels, ...19836418365
nature of hepatic damage induced by culture filtrates of seed-borne fungi in the laboratory rat.oral intubation of culture filtrates of aspergillus flavus and a. terreus produced hepatocellular damage in albino rats in the form of perilobular hypertrophy of the parenchymal cells and vacuolar degeneration of cytoplasm. focal necrosis and early periportal fibrosis were also evident.19836419399
[ecology of aflatoxin b1-producing aspergillus flavus strains]. 19836419486
a case of aflatoxicosis in fattening calves caused by aflatoxin formation in inadequate formic acid treated grain. 19836419504
development of aspergillus sinusitis in a patient receiving amphotericin b. treatment with granulocyte transfusions.fulminant aspergillus sinusitis is a disease of immunocompromised hosts strongly associated with neutropenia. a case of sinusitis due to aspergillus flavus that developed in a patient with acute leukemia during the third week of treatment with amphotericin b is described. indium 111-labeled white blood cell scanning demonstrated uptake of granulocytes into the involved sinuses. thereafter, use of granulocyte transfusions was associated with stabilization of the patient's clinical course.19846419603
aspergillus infection and aflatoxin production in some cowpea (vigna unguiculata (l.) walp) lines in tanzania.seeds of twenty-two cowpea (vigna unguiculata (l.) walp) lines/cultivars were inoculated with aspergillus parasiticus (link) (nrrl 3145) and aflatoxin production was evaluated. all the cowpea samples were susceptible to aspergillus infection and subsequent aflatoxin production. the amount of aflatoxin produced ranged between 466.6 micrograms/kg to 1 806 micrograms/kg in the case of b (b1 + b2) and 20.8 micrograms/kg to 82.7 micrograms/kg of seed in the case of g (g1 + g2). on the basis of the am ...19836422301
production of aflatoxins on soybean and cottonseed meals.p6e production of aflatoxins by aspergillus flavus srrc-1000 growing on soybean (forrest) and glandless cottonseed (deltapine 16) meals was examined with respect to effects of zinc and phytate. aflatoxins were not produced on unautoclaved soybean meal. addition of zinc (as zinc sulfate) to autoclaved meal inhibited aflatoxin production and supplementation with sodium phytate relieved this inhibition. addition of sodium phytate alone promoted production. when cottonseed meal was treated to releas ...19836422302
disseminated aspergillosis presenting with endophthalmitis. a case report and a review of the literature.a patient with idiopathic thrombocytosis developed sudden loss of vision in his left eye secondary to endophthalmitis caused by aspergillus flavus. he subsequently manifested other symptoms and signs of disseminated infection, and eventually died despite prompt initiation of appropriate parenteral antifungal therapy. a period of profound, iatrogenic neutropenia preceded the development of his terminal infection. previously reported cases of hematogenously acquired aspergillus endophthalmitis are ...19846423266
effect of contamination of foods by aspergillus flavus on the nutritive value of protein. 19846423892
stimulation of aflatoxin b1 and t-2 toxin production by sorbic acid.aspergillus flavus grown on yeast extract-sucrose medium produced higher amounts of aflatoxin b1 in the presence of 0.025% sorbic acid than without this chemical with a maximum at 17 days of incubation. addition of 0.05 to 0.0125% sorbic acid stimulated t-2 toxin production of fusarium acuminatum cultures grown on maize meal. the highest amounts of the mycotoxin were detected in 14-day-old cultures containing 0.025% sorbic acid. it is assumed that certain amounts of sorbic acid near the minimal ...19846424567
genetics of aspergillus flavus: linkage of aflatoxin mutants.eight aflatoxin (afl) mutants of aspergillus flavus were induced with n-methyl-n'-nitro-n-nitrosoguanidine. heterozygous diploids formed between afl mutants and tester strains revealed that each afl mutant was recessive. haploids selected from these heterozygous diploids indicated the linkage of all eight afl mutants to markers on group vii. these include previously mapped arg-7 (arginine), leu (leucine), dominant afl-1, and nor which accumulates norsolorinic acid that is visible as an orange-re ...19846424919
primary mycotic infection of paranasal sinuses.eight cases of primary mycotic infection of paranasal sinuses with aspergillus and candida species encountered during july, 1979 to december 1981 are being presented. their clinical, mycological and serological responses have been discussed. aspergillus flavus was isolated from all the seven cases with aspergillosis and candida albicans, along with c. tropicalis, was isolated from one case.19846425695
antemortem diagnosis of systemic aspergillosis: ten-year review and report of a case.a review of the medical literature over the last ten years indicates the rarity of antemortem diagnosis and treatment of systemic aspergillosis despite its rapid increase in incidence in the united states. we report a case of systemic aspergillosis caused by aspergillus flavus which was isolated from the blood of an immunosuppressed patient with chronic obstructive pulmonary disease. the fungus grew rapidly on brain-heart infusion medium and sabouraud dextrose agar cultures. autopsy examination ...19846426063
toxic effects of aflatoxin b1 in chickens given feed contaminated with aspergillus flavus and reduction of the toxicity by activated charcoal and some chemical agents.aspergillus flavus nrrl 3357 was grown on enriched long-grain rice for 7-10 days to produce aflatoxin b1 (afb1). the quantity of afb1 in moldy rice was determined by thin-layer chromatography using ultraviolet light. when the dried moldy rice powder was fed to day-old hubbard x hubbard broiler chicks in unmedicated feed (afb1 level 10 ppm) for 8 weeks, there was a profound reduction in weight gain and feed consumption. chickens fed afb1 developed severe liver damage, as determined by the concent ...19846426455
fungus growth on soft contact lenses with different water contents. 19846426818
effect of aluminium and nickel on aflatoxin production by aspergillus flavus.effect of nickel and aluminium was studied on aflatoxin and lipid production by two strains of aspergillus flavus in a sucrose-asparagine-salts medium. inclusion of aluminium in the medium established an inverse relationship between aflatoxin and lipid production. at lower concentrations aluminium stimulated aflatoxin production, whereas at higher concentrations it stimulated total lipid production. nickel at higher concentrations resulted in an increase in total aflatoxin production. however, n ...19846427072
aspergillic acids produced by mixed cultures of aspergillus flavus and aspergillus nidulans.a mixed culture of aspergillus nidulans (gh79) and aspergillus flavus (cmi 91019b) produced two antibiotics, designated vi and vii, which were not elaborated when either fungus was grown alone. chemical and spectroscopic analysis of vi, the major component, indicated that this compound was identical to hydroxyaspergillic acid. the minor component, vii, was produced in too low a yield for its identity to be established. however, partial characterization suggests that this antibiotic also belongs ...19846427396
effect of geocarposphere temperature on pre-harvest colonization of drought-stressed peanuts by aspergillus flavus and subsequent aflatoxin contamination.florunner peanuts grown in research plots were subjected to 5 soil temperature and moisture treatment regimes resulting in a. flavus infestation and subsequent aflatoxin contamination in drought-stressed peanuts. treatments imposed beginning 85 days after planting were drought, drought with heated soil and 3 drought treatments with cooled soil. the incidence of a. flavus in drought-stressed, unshelled , sound mature kernels ( smk ) decreased with decreases in the mean 5 cm deep soil temperature. ...19846427616
indium 111 wbc scan in local and systemic fungal infections.we describe two patients-one with a systemic fungal infection and one with a localized form-who had strikingly abnormal indium 111 leukocyte (wbc) scans. the patient with systemic disease had an abnormal wbc scan before lesions became clinically apparent.19846428343
serum ige as an important aid in management of allergic bronchopulmonary aspergillosis.differentiating patients with allergic bronchopulmonary aspergillosis (abpa) from patients with asthma who have immediate skin reactivity to aspergillus fumigatus may be difficult when the characteristic clinical and laboratory findings of abpa are absent. this article describes the response of abpa patients to corticosteroid therapy. after treatment of the acute stage of the disease with corticosteroid therapy, all abpa patients had greater than or equal to 35% reduction of the total serum ige ...19846429230
effect of soil temperature and drought on peanut pod and stem temperatures relative to aspergillus flavus invasion and aflatoxin contamination.peanut stem and pod temperatures of plants growing in irrigated, drought, drought-heated soil, and drought-cooled soil treatments were determined near the end of the growing season. mean soil temperatures of the treatments during this period were 21.5 degrees, 25.5 degrees, 30 degrees and 20 degrees c, respectively. peanut stem temperatures in all drought treatments reached a maximum of ca. 40 degrees c and for 6-7 h each day were as much as 10 degrees c warmer than irrigated peanut stems. pod t ...19846429541
the nucleotide sequences of the 5 s rrnas of seven molds and a yeast and their use in studying ascomycete phylogeny.the sequences of the 5 s rrnas isolated from 8 ascomycete species belonging to the genera aspergillus, penicillium, acremonium and candida are reported. two of the examined strains each yielded a mixture of 3 slightly different 5 s rnas, which were individually sequenced after fractionation. a previously published sequence for aspergillus nidulans 5 s rna was found to contain errors. reconstruction of an evolutionary tree based on 5 s rna sequences showed that the 16 presently examined ascomycet ...19846429642
[hospital aspergillosis]. 19846431721
invasive aspergillosis demonstrating the air crescent sign: occurrence in a previously healthy alcoholic. 19846433875
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