| detection of yeasts and filamentous fungi in blood cultures during a 10-year period (1972 to 1981). | during a 10-year period (january 1972 to december 1981), 69,066 fungal blood cultures were performed by using a biphasic brain heart infusion medium. a total of 838 fungi were recovered from 302 patients. candida species represented 71% (595) of all positive cultures, cryptococcus neoformans 12.6% (106), and histoplasma capsulatum 13.1% (110). | 1982 | 7153348 |
| isolation of histoplasma capsulatum from an armadillo (dasypus novemcinctus) in the eastern amazon of brazil. | | 1982 | 7179429 |
| visualization of histoplasma capsulatum in bone marrow with prussian blue iron stain. | bone marrow specimens from patients with disseminated histoplasmosis revealed yeast cells that stained positively with prussian blue. | 1982 | 7186903 |
| the mycelial status and reversibility in histoplasma capsulatum. | the highly variable mycelial phase cultures of histoplasma capsulatum are generally described as brown to albino colonies with the albino type overtaking the brown in a unidirectional and mainly irreversible process. in this study it was found that cultures could be reversed from albino to brown type by manipulation of substrates. as the cultural morphology and stability were shown to be essentially substrate-dependent, the mycelial status of this organism was reassessed. it is proposed that alb ... | 1980 | 7192886 |
| observations on the chemical compositions of some pathogenic fungi for humans determined by x-ray microanalysis. | the electron microprobe analyzer was used to study the chemical compositions of some pathogenic fungi for humans. elementary analysis was based on the characteristic x-ray spectra generated by the electrons passing through the specimens. this is a non-destructive method allowing to perform multiple elementary analysis in the same tissue. in order to increase our understanding of pathogenic fungi, small pieces of fungi tissue were analyzed. the species examined were histoplasma capsulatum, blasto ... | 1980 | 7203265 |
| histoplasmosis in the eastern caribbean: a preliminary survey of the incidence of the infection. | a preliminary survey of the incidence of histoplasmosis in the eastern caribbean was carried out. the percentages of positive histoplasmin skin test reactors in the populations studied were as follows: barbados (4%), trinidad (42%) and guyana (29%). in trinidad 69% of individuals under 60 had positive test. however a lower proportion (4%) had serological evidence of past infection with histoplasma capsulatum. the organism was not isolated from the soil although it had been found previously in tr ... | 1981 | 7206022 |
| histoplasma capsulatum epididymitis. | we report 2 cases of epididymal histoplasmosis. in 1 patient an epididymal abscess was the sole manifestation of histoplasmosis and in the other an epididymal abscess occurred with paratracheal lymphadenopathy. although uncommon histoplasmosis can cause symptomatic genitourinary tract disease and must be differentiated from tuberculosis, tumors and other fungal and bacterial infections. | 1981 | 7206103 |
| histoplasmosis of bone. | a case of histoplasmosis of bone manifested by a single punched-out lesion in the humerus is reported in a man with asymptomatic mild chronic disseminated histoplasmosis. roentgenologically evident lesions of bone due to histoplasma capsulatum are rare, and this is the only report of a large punched-out lytic lesion. such lesions are common in disseminated histoplasmosis due to the african strain h. duboisii. although h. duboisii has been accorded species differentiation, there are only two clea ... | 1981 | 7211920 |
| selenocystine-resistant mutants of histoplasma capsulatum. | cysteine metabolism has been thought to be important to the phenomenon of dimorphism in histoplasma capsulatum. we sought mutants with genetic blocks in the metabolism of cysteine by selection of colonies resistant to the toxic analogue, selenocystine. the 22 resistant strains thus obtained were all deficient in uptake of cystine from the surrounding medium but were normally able to convert from mycelium to yeast and back again. furthermore, they had normal quantities of nadh-dependent cystine r ... | 1981 | 7219517 |
| histoplasma capsulatum endocarditis. | | 1981 | 7226950 |
| acute pulmonary histoplasmosis presenting as adult respiratory distress syndrome: effect of therapy on clinical and laboratory features. | three patients with acute pulmonary histoplasmosis presented with extensive, diffuse bilateral infiltrates on chest roentgenograms. fungal elements were seen in the bronchial secretions of two patients; histoplasma capsulatum was grown from the third patient and from soil from the patients' workplace. two patients were severely hypoxemic and required short courses of amphotericin b therapy; in one of these two, progressive deterioration dictated corticosteroid therapy as well, with a dramatic cl ... | 1981 | 7244706 |
| human lymphocyte blastogenesis induced by living and dead histoplasma capsulatum yeasts and soluble yeast autolysate. | human peripheral blood mononuclear cells were cultured with either replicating yeast phase histoplasma capsulatum, intact dead yeasts of soluble yeast phase autolysate antigen for induction of lymphocyte blastogenesis. h. capsulatum replicated to the same extent in cultures of lymphocytes from histoplasmin skin test-positive and negative cell donors, but stimulated far greater blastogenesis in the former. dead h. capsulatum yeasts, which were more easily quantified, caused nearly as much specifi ... | 1981 | 7256491 |
| pathogenesis of experimental histoplasmosis in the bat, artibeus lituratus. | the pathogenesis of histoplasmosis was studied following intraperitoneal or intranasal infection of the neotropical bat, artibeus lituratus. groups of bats received either 10(4) or 10 viable mycelial fragments of histoplasma capsulatum by intraperitoneal injection, or 10(6) or 10(4) viable mycelial particles by intranasal instillation. intraperitoneal infection with the high dose resulted in rapid dissemination of the fungus to spleen, liver, lung and intestine, culminating in the death of some ... | 1981 | 7258485 |
| [infections in the child with acute leukemia]. | one hundred and twenty-five febrile episodes in 82 children with acute leukemia were studied; 46% of the patients were from urban and 54% from rural areas. the origin of the fever was identified in 91% of the episodes, prevailing pneumonia, septicemia, chickenpox and herpes zoster. the etiological agent was identified in 46% of the cases. a viral predominance was evident, and among them varicela-zoster, following in importance gram-negative bacteria. histoplasma capsulatum and pneumocystis carin ... | 1981 | 7259849 |
| hitchcock's birds, or the increased rate of exposure to histoplasma from blackbird roost sites. | a statewide survey of populations in proximity to blackbird roost sites to determine exposure to histoplasmosis from such sites has demonstrated that a site harboring histoplasma capsulatum, even though undisturbed, adds significantly to the exposure rate of proximal populations. disturbance of such a site increases the exposure rate dramatically with or without concurrent clinical cases of histoplasmosis. | 1981 | 7273893 |
| primary acute histoplasmosis in guinea pigs exposed to aerosolized histoplasma capsulatum. | guinea pigs were examined as a possible in vivo model for human histoplasmosis. guinea pigs were exposed to an aerosol of viable microconidia and mycelial fragments of histoplasma capsulatum generated in a henderson apparatus. colonization and infection of the lungs occurred, with subsequent involvement of the regional lymph nodes and reticuloendothelial organs. cultural recovery of the fungus from the nasopharynx and bronchoalveoli was initially high, but decreased with time. the mean number of ... | 1981 | 7275317 |
| prosthetic valve endocarditis caused by histoplasma capsulatum. | previously reported cases of histoplasma capsulatum infection of prosthetic heart valves have been first diagnosed at autopsy. a patient had an h capsulatum infection that was diagnosed by serologic means 1 1/2 years before involvement of the patient's prosthetic mitral valve was found at surgery. numerous previous attempts to demonstrate organisms by culture and histologic study of bone marrow and liver biopsy specimens were unsuccessful. various serologic tests were used in the diagnosis and e ... | 1981 | 7283568 |
| hydroxamic acid from histoplasma capsulatum that displays growth factor activity. | growth factor(s) present in a spent liquid medium after culture of the yeast form of histoplasma capsulatum enhanced both yeast and mycelial growth of nine isolates tested. hydroxamic acid extracted from the culture fluid displayed growth factor activity. | 1981 | 7294789 |
| interactions between histoplasma capsulatum and macrophages from normal and treated mice: comparison of the mycelial and yeast phases in alveolar and peritoneal macrophages. | interactions between macrophages (alveolar and peritoneal) from normal, vaccinated (with heat-killed yeast cells), and mycobacterium bovis bcg-treated mice and the mycelial and yeast phases of histoplasma capsulatum were observed. phagocytosis of microconidia, small hyphal fragments, and yeast cells occurred 4 to 6 h after the infection of macrophage cultures. conversion to the yeast phase began at 6 to 7 h and was complete after a 72-h incubation at 37 degrees c. macrophages surrounded and adhe ... | 1981 | 7298193 |
| electron cytochemical determinations of periodic acid-reactive sites in histoplasma capsulatum. | electron cytochemical determinations of periodic acid-thiocarbohydrazide-silver proteinate (patag)-reactive sites in the vegetative and reproductive cells of the pathogenic dimorphic fungus histoplasma capsulatum, are described an illustrated by electron micrographs. ultrastructural sites of patag-reactive substances were detected at the plasma membrane, along various cytomembranes of the cytoplasm, and in aggregates of storage material believed to be partly composed of glycogen or a glycogen-li ... | 1981 | 7300693 |
| outbreak of acute pulmonary histoplasmosis in members of a wagon train. | in august 1980, an outbreak of acute pulmonary histoplasmosis occurred among participants in a wagon train as it traveled through eastern tennessee. of the 85 people on the train 69 (81 percent) had evidence of infection with histoplasma capsulatum. fifty-four people had symptomatic disease. the source of infection was traced to the site of a former winter blackbird roost in charleston, tennessee, that had been partially cleared five years earlier to make a park. fourteen of 25 soil samples from ... | 1981 | 7304646 |
| ultrastructural studies on the dimorphism of paracoccidioides brasiliensis, blastomyces dermatitidis and histoplasma capsulatum. | whole cells or cell walls of the yeastlike and mycelial forms of paracoccidioides brasiliensis, blastomyces dermatitidis and histoplasma capsulatum were treated successively with sodium hydroxide, beta-1,3-glucanase and pronase. the microfibrils in the insoluble residues, probably composed of chitin, were examined in the electron microscope. in the yeastlike form, tightly interwoven, randomly oriented microfibrils were seen. on the other hand, in the mycelial form, a large portion of microfibril ... | 1981 | 7323909 |
| [multiple brain histoplasmomas: autopsy study of a case]. | report of the case of a 28-years-old black woman who developed changes in behavior followed by neurological alterations. the ct scans of the brain revealed multiple calcified granulomas. the patient died and the autopsy showed granulomatous lesions with central caseation in the brain. microscopical examination disclosed numerous histoplasma capsulatum. this is the first case of histoplasmoma of the brain described in brazil. | 1981 | 7325875 |
| acute histoplasmosis. description of an outbreak in northern louisiana. | an outbreak of acute histoplasmosis occurred in northern louisiana in september 1977. all six men who cleared a field of bamboo cane known to be a blackbird roosting site were affected. all had clinical and roentgenographic findings of acute pulmonary histoplasmosis, and all recovered. seroconversion or fourfold rise in antibody to histoplasma capsulatum yeast--or mycelial-form antigens were demonstrated in five. immunodiffusion testing showed that all six patients developed h capsulatum m bands ... | 1980 | 7351143 |
| histoplasma meningitis: diagnostic value of cerebrospinal fluid serology. | two patients with culture-negative chronic meningitis were diagnosed as having histoplasma capsulatum meningitis based on serial serologic studies; both had antibody in the cerebrospinal fluid as well as the serum. the patients were treated successfully with amphotericin b and had favorable clinical responses. three control patients with active histoplasmosis and positive serum serologic tests, but without meningeal involvement, did not have antibody in the cerebrospinal fluid. patients with chr ... | 1980 | 7352724 |
| amphotericin b-induced changes in k+ content, viability, and ultrastructure of yeast-phase histoplasma capsulatum. | yeast-phase cells of histoplasma capsulatum were challenged with amphotericin b, and membrane perturbation was monitored by k+ efflux. suspensions of washed cells readily absorbed about 1.12 microgram of amphotericin b per mg (dry weight) and further nonspecific sites were also apparent. the dose-response curve for initial rate of k+ efflux was sigmoidal within the range 0.1 to 1.0 microgram of amphotericin b per ml. a fungistatic concentration of amphotericin b (0.3 microgram/ml) evoked an effl ... | 1980 | 7354001 |
| cysteine transport and sulfite reductase activity in a germination-defective mutant of histoplasma capsulatum. | a mutant of the dimorphic, zoopathogenic fungus histoplasma capsulatum, defective in the ability of its blastospores to germinate, was used to show that the nutritional requirement for cysteine and the expression of sulfite reductase activity are temperature dependent and not related to the growth form the fungus, whereas the kinetics of cysteine transport depend on both temperature and the form of the fungus. | 1980 | 7354005 |
| association of hla-drw2 antigen with presumed ocular histoplasmosis. | patients with presumed ocular histoplasmosis syndrome were typed for hla-a, b, c, and dr antigens. it was found that 21 (81%) of 26 of the presumed ocular histoplasmosis syndrome patients with disciform macular scarring and 8 (62%) of 13 of the presumed ocular histoplasmosis syndrome patients with peripheral atrophic spots typed for drw2. the frequency of drw2 in normal blood donors was 28%. the 77% (20 patients) frequency of b7 in 26 of the presumed ocular histoplasmosis syndrome patients with ... | 1980 | 7356790 |
| disseminated canine histoplasmosis: a clinical survey of 24 cases in texas. | the clinical features of 24 cases of disseminated canine histoplasmosis are presented. the enteric form predominated and the age at presentation was from five months to ten years. the principal clinical findings were chronic diarrhea, weight loss, pyrexia and anemia.a premortem diagnosis was reached in 20 cases, by demonstrating histoplasma capsulatum organisms in peripheral blood smears, rectal scrapings or surgical biopsies. five of seven dogs treated with amphotericin b were released in asymp ... | 1980 | 7363273 |
| balanced growth and morphogenesis of histoplasma capsulatum in a defined synthetic medium. | we have characterized balanced growth of yeast and hyphal cells of histoplasma capsulatum and cells during transition from yeast to hyphae in a newly developed synthetic medium, r3b3. homogeneous populations of yeast at 37 degrees c and hyphae at 25 degrees c grew in this medium with generation times of 10h and 19h, respectively. the growth rates were exponential, as demonstrated by the kinetics of net increase in dry weight. identical rates of net increase were observed for ribonucleic acid (rn ... | 1980 | 7368043 |
| comparison of the ribonucleic acid polymerases from both phases of histoplasma capsulatum. | the three ribonucleic acid (rna) polymerases (ribonucleoside triphosphate rna nucleotidyltransferases, ec 2.7.7.6) of the two phases (yeast and mycelial) of the dimorphic fungus histoplasma capsulatum have been purified and characterized. the corresponding enzymes from the two phases differ in sensitivity to alpha-amanitin, ion and salt requirements, temperature sensitivity, and subunit structure. this is the first case in which such qualitative differences in rna polymerases have been demonstra ... | 1980 | 7370225 |
| multifocal ocular histoplasmosis in a dog and cat. | in two cases of naturally occurring disseminated histoplasmosis, multifocal inflammatory lesions were seen in the posterior segment of the eyes. histologic examination of both animals revealed lesions of active choroiditis (cat) and retinitis (dog) in association with numerous histoplasma capsulatum. | 1980 | 7372539 |
| histoplasma capsulatum endocarditis requiring aortic valve replacement for aortic insufficiency. | a patient with valvular heart disease was found to have disseminated histoplasmosis and histoplasma endocarditis. refractory congestive heart failure secondary to severe progressive aortic insufficiency and the presence of a large valvular vegetation necessitated aortic valve replacement, which was timed to coincide with the midpoint of a course of therapy with 4 gm of amphotericin b. the patient has done well since the completion of treatment. | 1980 | 7375995 |
| histoplasma capsulatum endocarditis. | endocarditis is a rare manifestation of disseminated histoplasma capsulatum infection. a 22-year-old man presented with a seven month history of fever, weight loss, and progressive aortic insufficiency. the diagnosis of h. capsulatum was suggested by a diagnostic rise in complement fixation titers and positive echocardiographic findings. the diagnosis was confirmed prior to surgery by positive bone marrow culture. progressive congestive heart failure necessitated replacement of the aortic valve ... | 1980 | 7377100 |
| mating types and convertibility among soil isolates of histoplasma capsulatum. | | 1980 | 7383051 |
| susceptibility of zoopathogenic fungi to phytoalexins. | phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. they are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. employing a broth dilution technique, we have found the zoopathogens petriellidium boydii, aspergillus flavus, a. fumigatus, candida albicans, coccidioides immitis, crypto ... | 1980 | 7387136 |
| [a case of disseminated histoplasmosis due to histoplasma capsulatum (author's transl)]. | a case is reported of american histoplasmosis due to histoplasma capsulatum presenting as a hepatic granulomatosis. the patient was a young adult from haiti and investigations revealed the presence of an ulcerous enterocolitic suggesting tuberculosis or crohn's disease. the condition became worse after antituberculous therapy and corticoids, and operation was necessary. extemporaneous histological examination, of the colonic ulcerations showed histoplasma capsultaum yeasts. the patient, who had ... | 1980 | 7396328 |
| interaction of histoplasma capsulatum with human platelets. | thrombocytopenia is a common accompaniment of disseminated histoplasmosis. the yeast form of histoplasma capsulatum does not directly injure human platelets freed of plasma. preincubation of h. capsulatum with plasma enabled it to induce prompt platelet aggregation and selective release of [3h]serotonin without release of lysosomal beta-glucuronidase and the cytoplasmic marker, lactate dehydrogenase. platelet aggregation was mediated by adenosine diphosphate, as shown by the blocking of the reac ... | 1980 | 7400626 |
| transferrin-dependent growth inhibition of yeast-phase histoplasma capsulatum by human serum and lymph. | nonspecific host defense mechanisms that may limit growth of yeast-phase histoplasma capsulatum in vivo were examined using an in vitro system of cell-free liquid culture. native human transferrin in serum and lymph, or purified transferrin added to serum-free medium, inhibited yeast replication 10- to 50-fold. supplementation of serum with iron to complete or almost complete saturation of total iron-binding capacity neutralized inhibition. substitution of zn++, mn++, or cu++ for fe++ did not af ... | 1980 | 7410898 |
| immunology of histoplasmosis: humoral and cellular activity from a polysaccharide-protein complex and its deproteinized fraction in experimentally immunized mice. | in a previous publication it was reported that a polysaccharide-protein complex (ppc), sensitive to beta-glucosidase, was isolated from histoplasma capsulatum. this complex was strongly reactive in an agar gel diffusion assay with sera from patients with histoplasmosis, but was unreactive with sera from patients with coccidioidomycosis. here, the studies with human sera have been expanded and attempts were made to determine the response of mice immunized with nonviable h. capsulatum or coccidioi ... | 1980 | 7412854 |
| histoplasma capsulatum endocarditis cured by amphotericin b combined with surgery. | a patient is reported who had histoplasma capsulatum endocarditis, a rare form of disseminated histoplasmosis. he also had disseminated intravascular coagulopathy and defects in cell-mediated immune responses to histoplasma antigens. prompt etiologic diagnosis was made by stain of tissue from a mouth ulcer and confirmed by culture of the organism from blood. an echocardiogram compared to a previous normal echocardiogram indicated changes consistent with a vegetation. histologic examination of th ... | 1980 | 7428470 |
| activation of the alternative complement pathway by histoplasma capsulatum. | the present study was performed to determine whether histoplasma capsulatum (yeast phase) is able to activate the alternative complement pathway. h. capsulatum yeasts were shown to consume c3 in c4-deficient guinea pig serum. immunoelectrophoretic conversion of alternative pathway component factor b confirmed that c3 consumption was mediated by activation of the alternative pathway. these results demonstrate that h. capsulatum is able to activate the alternative complement pathway. | 1980 | 7439970 |
| experimental inflammatory serous detachment of the retina. | rhesus monkey eyes were studied after the development of inflammatory serous retinal detachments following internal carotid artery injection of live, yeast-phase histoplasma capsulatum organisms. horseradish peroxidase (hrp) was used as a tracer to examine the inner and outer blood-retinal barriers. the hrp was present in a decreasing gradient from the choroid into the subretinal space and was not found to have leaked from retinal vessels. the source of subretinal fluid in this model of inflamma ... | 1980 | 7447779 |
| radioimmunoassays that use 125i-labeled protein a for determination of histoplasma capsulatum-specific immunoglobulin g, a, and m class antibodies in histoplasmosis. | a radioimmunoassay for determination of human immunoglobulin g, a, and m class antibodies to histoplasma capsulatum is reported. yeast-phase antigen from h. capsulatum was adsorbed onto polyvinyl chloride microtiter plates as the solid phase. human antibodies were allowed to react with this antigen, followed by addition of rabbit anti-human alpha- or mu-heavy chain sera. 125i-labeled staphylococcal protein a was then added and allowed to bind the human and rabbit immunoglobulin g. with this tech ... | 1981 | 7462420 |
| non-specific incorporation of nucleic acid precursors in blastomyces dermatitidis and histoplasma capsulatum. | incorporation of thymidine, thymidine monophosphate (tmp), thymidine triphosphate (ttp), uridine and orotic acid into dna, rna and protein in blastomyces dermatitidis and histoplasma capsulatum was studied utilizing a specific acid hydrolysis technique developed for these fungi. thymidine was incorporated to the greatest extent (approximately 0.5% of added label) followed by uridine, orotic acid, tmp and ttp. in blastomyces, uridine and orotic acid labeled primarily rna. tmp and ttp labeled rna, ... | 1980 | 7464897 |
| bronchoalveolar lavage for diagnosis of fungal disease. five years' experience in a southern united states rural area with many blastomycosis cases. | to determine the relative frequency of blastomyces dermatitidis among other microorganisms in bronchoalveolar lavage (bal) specimens. | 1995 | 7483983 |
| inhibition of growth of histoplasma capsulatum yeast cells in human macrophages by the iron chelator vuf 8514 and comparison of vuf 8514 with deferoxamine. | histoplasma capsulatum requires intracellular iron to survive and multiply within human and murine macrophages (m phi). thus, iron chelators may be useful compounds in the treatment of histoplasmosis. in the present study we compared the efficacies of five different iron chelators with deferoxamine (def) for their capacity to inhibit the growth of h. capsulatum yeast cells in culture medium and within human m phi. of the agents tested, only one, vuf 8514, a 2,2'-bipyridyl analog, was found to be ... | 1995 | 7486926 |
| cutaneous histoplasmosis in a child with hyper-igm. | immunodeficiency with hyperimmunoglobulinemia m is a rare disease characterized by very low levels of igg and iga and normal or high levels of serum igm and igd. recurrent and severe systemic infections with pathogenic bacteria are frequent if immunoglobulin replacement therapy is not given. histoplasmosis is a systemic granulomatous mycosis due to histoplasma capsulatum and characterized by a particular affinity for the reticuloendothelial system. glabrous skin involvement in histoplasmosis is ... | 1995 | 7501554 |
| expression of inducible nitric oxide synthase by stimulated macrophages correlates with their antihistoplasma activity. | the antihistoplasma activity of recombinant murine gamma interferon (rmuifn-gamma)-treated macrophages of the raw 264.7 cell line depends on the generation of nitric oxide (no.) from l-arginine. macrophages of the p388d1 cell line treated with rmuifn-gamma do not produce no. or inhibit the intracellular growth of histoplasma capsulatum. no. is generated by the inducible enzyme nitric oxide synthase (inos) formed by stimulated macrophages. northern (rna) blot analysis of raw 264.7 cells revealed ... | 1994 | 7510670 |
| cytologic and differential diagnosis of rhinosporidiosis. | rhinosporidiosis is a mycotic infection caused by rhinosporidium seeberi. the fungus occurs in tissues as spherules measuring 0.25-3 mm. the spherules contain endospores. diagnosis is usually made histologically on biopsy specimens from polypoid lesions on the mucous membranes of the nasopharynx, larynx, trachea, bronchus and conjunctiva. in our experience two cases of rhinosporidiosis were diagnosed by cytology. the cytologic features are typical. on direct examination the spherules are well-ci ... | 1994 | 7514831 |
| cryptococcus neoformans fails to induce nitric oxide synthase in primed murine macrophage-like cells. | nitric oxide (no) is a microbiostatic gas generated by activated murine macrophages. cytokine signals, gamma interferon (ifn-gamma) and tumor necrosis factor alpha (tnf-alpha) act synergistically to induce production of a macrophage nitric oxide synthase (nos). a variety of intracellular pathogens, when recognized by macrophages primed with ifn-gamma, induce nos by eliciting tnf-alpha secretion, which then functions as a positive autocrine signal. in cell culture assays, a murine macrophage cell ... | 1995 | 7534274 |
| antifungal mechanisms of activated murine bronchoalveolar or peritoneal macrophages for histoplasma capsulatum. | the first line of defence against natural infection by histoplasma capsulatum (hc) consists of bronchoalveolar macrophages (bam) and an early inflammatory response in the lungs. little is known about the interaction of bam and hc, consequently we studied murine bam in vitro to assess their role in the pulmonary defence in histoplasmosis. a short-term 3-h assay was used to measure fungicidal activity of control bam and interferon-gamma (ifn-gamma) plus lipopolysaccharide (lps)-activated bam. fung ... | 1995 | 7554402 |
| development of specific fluorescent-antibody test for tissue form of penicillium marneffei. | the diagnosis of penicilliosis marneffei can be difficult because the clinical manifestations mimic those of tuberculosis, histoplasmosis, and other mycotic infections. furthermore, the tissue form of penicillium marneffei can be confused with those of histoplasma capsulatum and cryptococcus neoformans. to facilitate the rapid detection and identification of p. marneffei in clinical materials, we sought to develop a specific indirect fluorescent-antibody (ifa) reagent for this dimorphic pathogen ... | 1995 | 7559962 |
| genetic diversity in clinical isolates of the dimorphic fungus blastomyces dermatitidis detected by a pcr-based random amplified polymorphic dna assay. | blastomyces dermatitidis is a dimorphic fungus causing localized or systemic infection in areas where the organism is endemic in the central and southeastern united states. in this study, 19 independent isolates of b. dermatitidis from little rock, ark., were grouped into three classes based on restriction fragment length polymorphism patterns in mitochondrial dna with a heterologous probe from histoplasma capsulatum. one large class of 15 isolates and two smaller classes (classes 2 and 3), each ... | 1995 | 7559971 |
| [disseminated histoplasma capsulatum histoplasmosis in african aids patients (3 cases)]. | the authors describe three cases of histoplasma capsulatum histoplasmosis that occurred in black aids patients living in france but originally from guinea and ivory coast. in all three cases histoplasmosis was disseminated with fever. in two cases there were cutaneous manifestations. one patient had renal insufficiency and nephrotic syndrome and another presented ulcerative colitis with histoplasma in the chorion. the outcome was favorable in two patients. these three cases are in addition to th ... | 1995 | 7564997 |
| unraveling the secrets of histoplasma capsulatum. a model to study morphogenic adaptation during parasite host/host interaction. | early in the developmental period of microbiology, pasteur first observed the phenomenon of dimorphism in fungi when he noticed that the bread mold mucor grew as a filamentous mold aerobically on the surface of broth cultures but at the bottom of the flask where the environment was anaerobic it reproduced as budding yeast cells. several infectious fungal pathogens of humans, namely histoplasma capsulatum, blastomyces dermatitidis, paracoccidioides brasiliensis, sporothrix schenkii, and coccidioi ... | 1995 | 7571855 |
| [colonic histoplasma capsulatum pseudotumor in aids. morphological and immunohistochemical diagnosis of an isolated lesion]. | the authors report a case of a 35-year-old man with acquired immunodeficiency syndrome (aids) and a left colonic mass with histoplasma capsulatum (h. capsulatum). the look-up performed looking for disseminated infection was negative. in the absence of positive cultures, the diagnosis was determined morphologically based on the presence of yeast observed by light and electron microscopy. the diagnosis was also verified by positive immunofluorescence using specific anti-histoplasma antibodies. gas ... | 1995 | 7574912 |
| [african histoplasmosis due to histoplasma capsulatum var. duboisii: relationship with aids in recent congolese cases]. | six new cases are described for african histoplasmosis, histoplasma capsulatum var. duboisii, from congo. the first was an hiv sero-negative child who has been monitored for the last three years. while under treatment with ketoconazole, amphotericin b, and finally itraconazole, the development of the infection was accompanied by purulent lesions, mainly cutaneous, but also superficial and deep lymphadenopathies. as a last option, itraconazole gave very satisfactory results both during the acute ... | 1995 | 7582643 |
| il-12 prevents mortality in mice infected with histoplasma capsulatum through induction of ifn-gamma. | histoplasma capsulatum is a pathogenic fungus found in discrete geographic locations throughout the world. the fungus invades the reticuloendothelial organs such as the spleen and liver of immunocompetent hosts where it is usually controlled. however, in individuals with immune deficiency, histoplasmosis is a severe and potentially fatal disease. resistance to this infection is due primarily to a cellular immune response mediated by t cells and macrophages. moreover, ifn-gamma is critical in act ... | 1995 | 7608555 |
| histopathologic spectrum of aids-associated lesions in maharaj nakorn chiang mai hospital. | the histopathological alterations in various organs and the presence of aids-associated lesions were studied in 86 biopsy and 29 necropsy specimens of aids patients. the most common cancer seen in this study were malignant lymphomas (4% of cases) with development of extensive extranodal lymphomatous involvement from the outset. although a preponderance of high grade b-cell pathologic subtypes is found in aids-associated lymphoma, we also report the first case of t-lymphoblastic lymphoma with a p ... | 1994 | 7612115 |
| the endemic mycoses: surgical considerations. | surgical consultation is regularly requested for diagnosis and treatment of pulmonary complications of the endemic mycosis, histoplasma capsulatum, blastomyces dermatitidis and coccidioidomycosis immitis, and the yeast cryptococcus neoformans. all resemble pulmonary malignancies. histoplasmosis causes pericarditis, mediastinal fibrosis and mediastinal granuloma, which can cause entrapment of vascular structures, the esophagus, and the trachea. coccidioidomycosis can cause spontaneous pneumothora ... | 1995 | 7612761 |
| histoplasmosis in northwestern argentina. ii: prevalence of histoplasmosis capsulati and paracoccidioidomycosis in the population south of chuscha, gonzalo and potrero in the province of tucuman. | the present work was undertaken to obtain epidemiological data on the extent and distribution of histoplasma capsulatum var. capsulatum and paracoccidiodes brasiliensis infections south of the chuscha, gonzalo and potrero areas of argentina. skin tests surveys of the human population with histoplasmin and paracoccidioidin were carried out in the permanent population of those localities. the infection index of the population showed that the area south of chuscha has a high-prevalence of histoplas ... | 1995 | 7617013 |
| study of fc gamma murine macrophage receptors (fc gamma r) in the internalization of histoplasma capsulatum yeasts. | reports about fc gamma r involvement in the phagocytosis of histoplasma capsulatum yeast are contradictory. fungal cells were incubated with anti-histoplasma murine igg or normal igg as control. the opsonized yeasts were adjusted and spread on a j774.2 macrophage cell line monolayer. yeast cells were allowed to attach to the monolayers at 4 degrees c for 1 h prior to initiating the internalization step of the phagocytic process, by shifting the cells to 37 degrees c. the participation of fc gamm ... | 1995 | 7620285 |
| oral histoplasmosis in hiv-infected patients. a report of two cases. | histoplasmosis is a fungal infection caused by the organism histoplasma capsulatum. disseminated disease usually occurs in immunosuppressed patients or in patients with chronic illnesses. although relatively uncommon, histoplasmosis has been reported in patients with aids, and oral lesions have been noted on multiple sites and in various clinical presentations. we present two hiv-positive cases with oral lesions as the initial signs of histoplasmosis. both patients responded well to iv amphoteri ... | 1995 | 7621028 |
| immunobiological activity of recombinant h antigen from histoplasma capsulatum. | the h antigen from histoplasma capsulatum is one of the known principal antigens of this fungus, but information regarding its identity and its participation in cellular immunity is largely unavailable. therefore, we sought to determine both the nature of this antigen and the nature of its involvement in the cell-mediated immune response. the antigen was isolated from histoplasmin and digested with selected proteinases, and the cleavage products were subjected to reverse-phase high-performance l ... | 1995 | 7622243 |
| penicillium marneffei infection in patients infected with the human immunodeficiency virus. a report of two cases. | we present the cytologic and histologic features of two cases of penicillium marneffei (pm) infection occurring in human immunodeficiency virus-positive patients following a journey to endemic areas. pm closely resembles histoplasma capsulatum, in both its morphology and tissue reactions. distinctive histologic features and culture provide the definitive diagnosis. the presence of a pm infection in a human immunodeficiency virus-positive patient returning from an endemic area should be considere ... | 1995 | 7631559 |
| murine laminin binds to histoplasma capsulatum. a possible mechanism of dissemination. | histoplasmosis, an increasingly important opportunistic infection in immunosuppressed subjects, is characterized by hematogenous dissemination of the yeast from the lung. the mechanism of this dissemination is not fully understood. laminin, the major glycoprotein of the extracellular matrix, is known to mediate the attachment of various invasive pathogens to host tissues. in the current study, laminin is demonstrated to bind to histoplasma capsulatum in a rapid, specific, and saturable manner. s ... | 1995 | 7635937 |
| parasitic infections associated with hiv/aids in the caribbean. | this review article seeks to highlight the significance for the caribbean of major parasitic infections associated with aids, encourage awareness of these opportunistic parasites, and promote familiarity with appropriate diagnostic techniques and their clinical relevance. specific agents considered include pneumocystis carinii; toxoplasma gondii; the enteric coccidians cryptosporidium spp., isospora belli, and cyclospora cayetanensis; the hemoflagellates leishmania spp. and trypanosoma cruzi; th ... | 1995 | 7640691 |
| cloning and analysis of cdna encoding an elongation factor 1 alpha from the dimorphic fungus histoplasma capsulatum. | the cdna encoding translation elongation factor 1 alpha (ef-1 alpha) was isolated from the dimorphic fungus, histoplasma capsulatum (hc), an important pathogen of man. a cdna library was probed with the tef1 gene from the fungus mucor racemosus. ten independent clones were isolated, all with similar restriction patterns. the longest clone (1.96 kb) was sequenced. southern blot analysis revealed that the hc tef1 gene was present as a single copy. a single transcript of approx. 2300 nucleotides wa ... | 1995 | 7642125 |
| cloning and nucleotide sequence of a specific dna fragment from paracoccidioides brasiliensis. | we cloned and sequenced a species-specific 110-bp dna fragment from paracoccidioides brasiliensis. the dna fragment was generated by pcr with primers complementary to the rat beta-actin gene under a low annealing temperature. comparison of the nucleotide sequence, after excluding the primers, with those in the genbank database identified approximately 60% homology with an exon of a major surface glycoprotein gene from pneumocystis carinii and a fragment of unknown function in saccharomyces cerev ... | 1995 | 7650207 |
| killing of histoplasma capsulatum by macrophage colony stimulating factor-treated human monocyte-derived macrophages: role for reactive oxygen intermediates. | the interaction of human macrophages with the yeast-form of histoplasma capsulatum was studied. the use of culture and a short-term assay period instead of microscopy gave direct evidence of the fungicidal activity of human macrophages. the present study reports the novel finding of fungicidal activity of macrophages derived from monocytes in the presence of macrophage colony-stimulating-factor (mcsf). the induction of fungicidal activity by this cytokine was dose dependent. mcsf at 10,000 u/ml ... | 1995 | 7650732 |
| [histoplasmosis, caused by histoplasma capsulatum, and aids]. | histoplasma capsulatum histoplasmosis occurs frequently in endemic areas and with the aids outbreak, it appears as an opportunistic fungus involved in disseminated disease. we report the clinical, biological and treatment features of seven cases diagnosed in the cisih of the eastern part of paris. clinically, four patients were suffering from pulmonary symptoms, in three cases digestive disorders and in three cutaneous lesions. in all cases, the mycologic diagnosis was necessary. amphotericin b ... | 1995 | 7652222 |
| induction of novel protein synthesis by opsonized histoplasma capsulatum ingested by murine peritoneal macrophages. | it is known that histoplasma capsulatum can resist the intraphagolysosomal environment and multiply inside macrophages. this resistance can be closely related to its pathogenicity. the mechanism of this resistance has been investigated, but it has not been clarified as yet. to learn about the metabolic condition of the yeast-form of h. capsulatum (isolates g217b and cdc 105) when ingested by macrophages, we investigated protein synthesis by ingested h. capsulatum with [35s]-methionine labeling. ... | 1995 | 7659141 |
| histoplasmosis: the otolaryngologist's perspective. | within the upper aerodigestive tract, histoplasmosis often mimics carcinoma, making prompt and accurate diagnosis imperative. more severe and potentially lethal infections with histoplasma capsulatum are now being seen as the numbers of patients at the extremes of age, as well as those with compromised immune systems, increase. we reviewed the cases of 115 hospitalized patients with disseminated histoplasmosis. of these, 9 patients were identified with otolaryngologic manifestations: 4 were infe ... | 1995 | 7666725 |
| histoplasmosis capsulati and duboisii in europe: the impact of the hiv pandemic, travel and immigration. | the present report describes a fatal case of imported aids-related disseminated histoplasmosis capsulati infection associated with multiple coexisting infections, diagnosed with cultural recovery of histoplasma capsulatum var. capsulatum with a commercial radiometric mycobacterium medium. the epidemiological and clinical features of histoplasmosis capsulati and duboisii in europe are reviewed by examining also 69 documented cases of histoplasma capsulatum var. capsulatum infection (25 in aids pa ... | 1994 | 7672046 |
| histoplasmosis--kentucky, 1995. | histoplasmosis is an infection resulting from inhalation of spores from the dimorphic fungus histoplasma capsulatum; the condition primarily affects the lungs. during august 1995, the department for health services, kentucky cabinet for human resources (kdhs), and local public health officials investigated two unrelated outbreaks of acute histoplasmosis in eastern kentucky. this report summarizes preliminary findings of the investigations of these outbreaks. | 1995 | 7675016 |
| responses of t and b lymphocytes to a paracoccidioides brasiliensis cell wall extract in healthy sensitized and nonsensitized subjects. | antigen-specific cellular immunity in paracoccidioidomycosis (pcm) has been poorly studied due to lack of standard in vitro lymphocyte proliferation assays. to standardize such an assay, we studied t and b cell responses to a paracoccidioides brasiliensis cell wall extract (pbag) in healthy subjects sensitized to either p. brasiliensis [pb(+)hc(-)] or to histoplasma capsulatum [hc(+)pb(-)], and in nonsensitized persons. all subjects showed, as expected, a vigorous proliferative response to a con ... | 1995 | 7677223 |
| combined histochemical stains in the differential diagnosis of cryptococcus neoformans. | three combinations of histochemical stains were used to study 69 routinely processed tissues containing various "yeast-like" fungal organisms (cryptococcus neoformans, blastomyces dermatitidis, coccidioides immitis, histoplasma capsulatum, paracoccidioides brasiliensis, and candida albicans). fontana-masson stain combined either with mucicarmine or alcian blue produced distinctive staining of c. neoformans (fontana-masson-positive wall and mucin-positive capsule) that was not identified in other ... | 1993 | 7678937 |
| production of a murine monoclonal antibody that recognizes an epitope specific to coccidioides immitis antigen 2. | antigen 2 (ag2) has been implicated as a t-cell-reactive component of the pathogenic fungus coccidioides immitis. we report the production of a murine monoclonal antibody (mab) of the immunoglobulin g2a isotype that recognizes an epitope specific to c. immitis ag2. this specificity was evidenced by the finding that the mab did not recognize other antigens present in coccidioidin or spherulin and did not show reactivity with antigenic extracts from histoplasma capsulatum or blastomyces dermatitid ... | 1993 | 7682998 |
| phylogeny and pcr identification of the human pathogenic fungus penicillium marneffei. | the phylogenetic position of the human pathogenic fungus penicillium marneffei was assessed from the nucleotide sequences of the nuclear and mitochondrial ribosomal dna regions. phylogenetic analysis determined that p. marneffei is closely related to species of penicillium subgenus biverticillium and sexual talaromyces species with asexual biverticillate penicillium states. knowledge of the phylogenetic position of p. marneffei facilitated the design of unique oligonucleotide primers, from the n ... | 1995 | 7699073 |
| itraconazole treatment of disseminated histoplasmosis in patients with the acquired immunodeficiency syndrome. aids clinical trial group. | amphotericin b has been the treatment of choice for disseminated histoplasmosis in patients with acquired immunodeficiency syndrome (aids). oral antifungal agents would be welcome alternatives to standard treatment of disseminated histoplasmosis in less severe cases. the purpose of this study was to assess the efficacy and safety of itraconazole therapy in patients with aids and disseminated histoplasmosis. | 1995 | 7709945 |
| loss of renal allograft function caused by histoplasma capsulatum. | | 1995 | 7725517 |
| disseminated histoplasmosis presenting as myositis and fasciitis in a patient with dermatomyositis. | a 54-year-old man with dermatomyositis initially responsive to corticosteroids and methotrexate developed severe myalgias, increasing weakness, and fevers. laboratory studies were suggestive of disseminated histoplasmosis, and muscle biopsy revealed myositis, fasciitis, and yeast in the perimysial connective tissue. histoplasma capsulatum was cultured from skeletal muscle. despite antifungal therapy, necrotizing fasciitis progressed to gluteal abscess formation. disseminated histoplasmosis may p ... | 1995 | 7739641 |
| comparison of the bact/alert and isolator blood culture systems for recovery of fungi. | the recovery of fungi by the bact/alert (organon teknika corporation, durham, nc) and isolator (wampole laboratories, cranbury, nj) blood culture systems was compared by retrospective review of 2,174 matched cultures from 715 patients collected from april 1, 1993 to february 28, 1994. one hundred five isolates were recovered from 44 patients by one or both culture systems. all patients were fungemic with one organism. in all cases of fungemia, collection times for both culture systems were withi ... | 1995 | 7741115 |
| comparative evaluation of the premier enzyme immunoassay, micro-immunodiffusion and complement fixation tests for the detection of histoplasma capsulatum var. capsulatum antibodies. | a total of 178 sera, including 68 from proven cases of histoplasmosis (65 positive for the presence of histoplasma capsulatum var. capsulatum antibodies and three positive for antigen), 93 from patients with suspected histoplasmosis but with no laboratory evidence of h. capsulatum var. capsulatum infection, 14 from humans with heterologous fungal and non-fungal infections and three from normal individuals, were tested for igg h. capsulatum antibodies and m or m and h precipitins by enzyme immuno ... | 1994 | 7746288 |
| [an outbreak of acute pulmonary histoplasmosis among travelers to a bat-inhabited cave in brazil]. | histoplasmosis is known to be endemic in various parts of the world, especially in north and latin america. in japan, histoplasma capsulatum has rarely been isolated from the natural environment. to date, only seven cases of histoplasmosis have been reported in japan including some that were contracted in foreign countries. herein, we report the occurrence of acute histoplasmosis among japanese travelers who were exposed to bat guano in a cave near manaus, brazil. a group of 8 japanese travelers ... | 1995 | 7751754 |
| clinical evidence of spinal and cerebral histoplasmosis twenty years after renal transplantation. | disseminated infection with histoplasma capsulatum frequently involves the nervous system, but the cns process is generally not clinically apparent. we report an unusual case of a renal transplant recipient with long-standing immunosuppression who presented with clinical evidence of mass lesions in both his cerebral cortex and his spinal cord. findings of csf examination were normal, but stereotaxic biopsies of his cortical lesions demonstrated yeast forms and cultures of biopsy specimens yielde ... | 1995 | 7756497 |
| thoracic mycoses from endemic fungi: radiologic-pathologic correlation. | the endemic fungi histoplasma capsulatum, blastomyces dermatitidis, and coccidioides immitis are primary human pathogens whose major portal of entry is the respiratory tract. their clinical manifestations are categorized as acute, chronic or chronic progressive, or disseminated fungal disease. most acute pulmonary infections are self-limited, and many are asymptomatic. chronic, progressive, or disseminated disease is much less common and most often occurs in immunocompromised patients. the radio ... | 1995 | 7761632 |
| in vivo and in vitro effects of macrophage colony-stimulating factor (m-csf) on bronchoalveolar macrophages for antihistoplasmal activity. | the in vivo and in vitro effects of m-csf on bronchoalveolar macrophages (bam) activity against the intracellular fungal pathogen histoplasma capsulatum (hc) were studied. three days after a single subcutaneous (s.c.) dose of m-csf (2.5 mg/kg), enhanced ex vivo antifungal activity of bam was measured. bam from m-csf-treated cd-1 mice significantly (p < 0.01) inhibited the intracellular multiplication of hc yeast cells in 20 h assays compared to bam from control mice. this effect was not observed ... | 1995 | 7782153 |
| a chronic cavitary pulmonary histoplasmosis case from turkey. | histoplasmosis, a disease endemic to north america, is caused by histoplasma capsulatum. to the best of our knowledge, the chronic cavitary pulmonary histoplasmosis patient described here presents the first case in which the fungus was isolated from sputum culture in turkey. | 1995 | 7783278 |
| histoplasma capsulatum infection in three aids patients living in africa. | three aids patients are reported, 2 european and 1 african. all 3 developed histoplasma capsulatum infection while living in africa. two developed a pulmonary infiltrate, and 2 had skin lesions. one patient was successfully treated with itraconazole, 400 mg daily; in a second patient, itraconazole was partially successful; the third patient was lost to follow-up before adequate treatment could be started. histoplasma infection is probably an often undiagnosed complication in african aids patient ... | 1995 | 7784825 |
| vaccination with recombinant heat shock protein 60 from histoplasma capsulatum protects mice against pulmonary histoplasmosis. | his-62 is a glycoprotein that has been isolated from the cell wall and cell membrane fraction of the pathogenic fungus histoplasma capsulatum. it is a target of the cellular immune response to this fungus, and it protects mice against a lethal intravenous inoculum of h. capsulatum yeast cells. in this study, we cloned the gene encoding this antigen to reveal its biological nature and studied the immunological activity of recombinant antigen. the amino acid sequences of the nh2 terminus and inter ... | 1995 | 7790073 |
| efficacy of the triazole d0870 in a murine model of systemic histoplasmosis. | the efficacy of d0870 was studied in a murine model of systemic histoplasmosis and was compared with that of fluconazole. all regimens of d0870 (1 or 10 mg/kg of body weight given daily or 1, 10, or 100 mg/kg given every other day) and 100 mg of fluconazole per kg given daily provided complete protection from lethality. comparison of the number of viable histoplasma capsulatum yeasts remaining in the spleen or liver indicated that d0870 was superior to fluconazole in clearing and curing infectio ... | 1995 | 7793893 |
| involvement of anterior chamber angle structures in disseminated histoplasmosis: report of three cases. | this study describes the involvement of anterior chamber (ac) angle structures in patients with disseminated histoplasmosis. the postmortem eyes from three patients (aged 33, 41, and 42 years, respectively) with disseminated histoplasmosis, two of whom had acquired immunodeficiency syndrome, were examined by light microscopy using hematoxylin-eosin, periodic acid-schiff (pas), and gomori's methenamide silver (gms) stains. electron microscopy studies of the choroid were performed in one eye. sign ... | 1995 | 7795509 |
| a natural focus of histoplasma capsulatum var. duboisii is a bat cave. | the natural reservoir of histoplasma capsulatum var. duboisii, the etiological agent of histoplasmosis duboisii (african histoplasmosis) is not yet known. we report the isolation of h. capsulatum var. duboisii from soil admixed with bat guano and from the intestinal contents of a bat in a sandstone cave in a rural area, ogbunike in anambra state of nigeria. eight of 45 samples of soil admixed with bat guano yielded h. capsulatum var. duboisii. of the 35 bats belonging to the species nycteris his ... | 1994 | 7808510 |
| the histoplasma capsulatum antigen assay in disseminated histoplasmosis in children. | progressive disseminated histoplasmosis is often fatal without treatment and requires rapid and accurate laboratory diagnosis. radioimmunoassay for histoplasma capsulatum var. capsulatum antigen has been established as a sensitive and accurate diagnostic technique for disseminated histoplasmosis in adults; this study examines the radioimmunoassay in children. the clinical and laboratory records of 26 patients 18 years old or younger in whom h. capsulatum antigen was detected in urine by radioimm ... | 1994 | 7808850 |
| rapid method for detection of aspergillus 5s ribosomal rna using a genus-specific oligonucleotide probe. | ribosomal rna (rrna) is present in all prokaryotic and eukaryotic cells. although sequences are conserved, variations in nucleic acid composition are known to be species specific. formalin or bouin's fixed, paraffin-embedded tissue specimens from 17 cases of culture proven aspergillus sp infections were studied by a rapid (< 30 minutes) in situ hybridization procedure using a biotinylated oligonucleotide dna probe complementary to nucleic acids 1-22 of aspergillus sp 5s rrna sequence. positivity ... | 1995 | 7817944 |
| macrophages from human immunodeficiency virus-positive persons are defective in host defense against histoplasma capsulatum. | the phagocytic and fungistatic activity of monocyte-derived macrophages from human immunodeficiency virus (hiv)-positive persons against histoplasma capsulatum yeasts was determined. macrophages from hiv-positive patients were profoundly deficient in their capacity to recognize and bind h. capsulatum, but ingestion of bound yeasts was normal. the binding of h. capsulatum by patient macrophages tended to decrease with a decrease in cd4+ t lymphocyte counts. another major defect was that patient m ... | 1995 | 7844367 |
| phylogeny of dermatophytes and dimorphic fungi based on large subunit ribosomal rna sequence comparisons. | the phylogeny of dermatophytes and dimorphic fungi was considered using the large-subunit of ribosomal rna (25s rrna). aligned sequences of 595 nucleotides covering the two most divergent domains d1 and d2, permitted a comparison of phylogenetic relationships at different levels. the dimorphic species (onygenaceae) were significantly separated from dermatophytes (arthrodermataceae) and from a third group including geophilic or very weakly pathogenic species (onygenaceae and gymnoascaceae). on a ... | 1994 | 7844699 |
| cutaneous cryptococcosis with molluscum contagiosum coinfection in a patient with acquired immunodeficiency syndrome. | we report the first patient with acquired immunodeficiency syndrome in whom molluscum contagiosum and cryptococcus neoformans were documented in the same cutaneous lesion. while cases of the co-occurrence of two pathologic entities in patients with acquired immunodeficiency syndrome have been reported, namely cytomegalovirus with herpes simplex virus and kaposi's sarcoma with histoplasma capsulatum, the findings in this patient are unique. the clinical presentation of cutaneous lesions caused by ... | 1994 | 7851129 |