| evaluation of galactose adapted yeasts for bioethanol fermentation from kappaphycus alvarezii hydrolyzates. | bioethanol was produced from kappaphycus alvarezii seaweed biomass using separate hydrolysis and fermentation (shf). pretreatment was evaluated for 60 min at 121°c using 12% (w/v) biomass slurry with 364 mm h2so4. enzymatic saccharification was then carried out at 45°c for 48 h using celluclast 1.5 l. ethanol fermentation with 12% (w/v) k. alvarezii hydrolyzate was performed using the yeasts saccharomyces cerevisiae kctc1126, kluyveromyces marxianus kctc7150, and candida lusitaniae atcc42720 wit ... | 2016 | 27056472 |
| indigenous georgian wine-associated yeasts and grape cultivars to edit the wine quality in a precision oenology perspective. | in georgia, one of the most ancient vine-growing environment, the homemade production of wine is still very popular in every rural family and spontaneous fermentation of must, without addition of chemical preservatives, is the norm. the present work investigated the yeast biodiversity in five georgian areas (guria, imereti, kakheti, kartli, ratcha-lechkhumi) sampling grapes and wines from 22 different native cultivars, in 26 vineyards and 19 family cellars. one hundred and eighty-two isolates we ... | 2016 | 27047468 |
| efficient conversion of xylose to ethanol by stress-tolerant kluyveromyces marxianus bunl-21. | the fermentation ability of thermotolerant kluyveromyces marxianus bunl-21 isolated in laos was investigated. comparison with thermotolerant k. marxianus dmku3-1042 as one of the most thermotolerant yeasts isolated previously revealed that the strain possesses stronger ability for conversion of xylose to ethanol, resistance to 2-deoxyglucose in the case of pentose, and tolerance to various stresses including high temperature and hydrogen peroxide. k. marxianus bunl-21 was found to have ethanol f ... | 2016 | 27026881 |
| effects of glucose, ethanol and acetic acid on regulation of adh2 gene from lachancea fermentati. | background. not all yeast alcohol dehydrogenase 2 (adh2) are repressed by glucose, as reported in saccharomyces cerevisiae. pichia stipitis adh2 is regulated by oxygen instead of glucose, whereas kluyveromyces marxianus adh2 is regulated by neither glucose nor ethanol. for this reason, adh2 regulation of yeasts may be species dependent, leading to a different type of expression and fermentation efficiency. lachancea fermentati is a highly efficient ethanol producer, fast-growing cells and adapte ... | 2016 | 26989608 |
| evaluation of hyper thermal acid hydrolysis of kappaphycus alvarezii for enhanced bioethanol production. | hyper thermal (ht) acid hydrolysis of kappaphycus alvarezii, a red seaweed, was optimized to 12% (w/v) seaweed slurry content, 180mm h2so4 at 140°c for 5min. the maximum monosaccharide concentration of 38.3g/l and 66.7% conversion from total fermentable monosaccharides of 57.6g/l with 120gdw/l k. alvarezii slurry were obtained from ht acid hydrolysis and enzymatic saccharification. ht acid hydrolysis at a severity factor of 0.78 efficiently converted the carbohydrates of seaweed to monosaccharid ... | 2016 | 26950757 |
| functional elucidation of the non-coding rnas of kluyveromyces marxianus in the exponential growth phase. | non-coding rnas (ncrnas), which perform diverse regulatory roles, have been found in organisms from all superkingdoms of life. however, there have been limited numbers of studies on the functions of ncrnas, especially in nonmodel organisms such as kluyveromyces marxianus that is widely used in the field of industrial biotechnology. | 2016 | 26923790 |
| effects of acetate on kluyveromyces marxianus dsm 5422 growth and metabolism. | metabolically active cells produce a wide array of metabolites that can inhibit their growth. acetate is a widely known preservative, and it is also produced by yeast cells during their growth. kluyveromyces marxianus dsm 5422 is a promising yeast strain that could be employed in biotechnological processes, but the knowledge of its stress physiology is scarce. here, we investigate the effects of acetate on growth and changes in cell population structure during adaptation to elevated concentratio ... | 2016 | 26910042 |
| enhancement in xylose utilization using kluyveromyces marxianus nire-k1 through evolutionary adaptation approach. | the evolutionary adaptation was carried out on the thermotolerant yeast kluyveromyces marxianus nire-k1 at 45 °c up to 60 batches to enhance its xylose utilization capability. the adapted strain showed higher specific growth rate and 3-fold xylose uptake rate and short lag phase as compared to the native strain. during aerobic growth adapted yeast showed 2.81-fold higher xylose utilization than that of native. in anaerobic batch fermentation, adapted yeast utilized about 91% of xylose in 72 h an ... | 2016 | 26886223 |
| the bioconversion of pretreated cashew apple bagasse into ethanol by shf and ssf processes. | ethanol production from acidic-alkaline pretreated cashew apple bagasse (cab-oh) was investigated using separated hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes. first, a screening of kluyveromyces strains was conducted by shf and a maximum ethanol concentration of 24.1 g l(-1) was obtained using kluyveromyces marxianus atcc36907, which presented similar profiles when compared to results obtained by a saccharomyces strain. the effect of tempe ... | 2016 | 26634842 |
| bioethanol production from taro waste using thermo-tolerant yeast kluyveromyces marxianus k21. | in the present study, evaluation and optimization of taro waste (tw), which was mainly composed of taro peels that contain many starch residues, as the main carbon source in medium were studied. the flask studies showed the optimal medium was using 170g/l of tw which is about 100g/l of glucose and 9g/l of cgm as alternative nitrogen source. simultaneous saccharification and fermentation (ssf) exhibited higher bioethanol productivity toward separation hydrolysis and fermentation (shf). the optima ... | 2016 | 26615498 |
| [research of uv-induced changes in the structural and functional properties of inulinases]. | uv-induced changes in the catalytic activity and radiuses of inulinases molecules from various producers (plants, fungy, yeast) are studied. it is established that specific enzymes activity and the sizes of inulinases molecules from helianthus tuberosus and kluyveromyces marxianus under the influence of uv-light in the ranges of doses 4530-6040 and 755-6040 j/m2, respectively, are subjected to changes more than structural and functional characteristics of inulinase fromaspergillus niger. it is p ... | 2016 | 26601544 |
| [heterologous expression, purification and characterization of exo-inulinase from kluyveromyces marxianus yx01]. | to improve the inulinase application in biotechnology, the characteristic of inulinase from kluyveromyces marxianus yx01 was investigated. the inu gene of k. marxianus yx01 was transformed into pichiapastoris gs115 host cells with molecular biology techniques. then we achieved the heterologous expression of exo-inulinase whose molecular mass was about 86.0 kda by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). furthermore, six his-tag was added to the inulinase and a two-st ... | 2015 | 26571688 |
| probiotic yeast kluyveromyces marxianus cidca 8154 shows anti-inflammatory and anti-oxidative stress properties in in vivo models. | inflammatory bowel diseases (ibds) are complex affections with increasing incidence worldwide. multiple factors are involved in the development and maintenance of the symptoms including enhanced oxidative stress in intestinal mucosa. the conventional therapeutic approaches for ibds are based on the use anti-inflammatory drugs with important collateral effects and partial efficacy. in the present work we tested the anti-inflammatory capacity of kluyveromyces marxianus cidca 8154 in different mode ... | 2016 | 26565081 |
| survival of the functional yeast kluyveromyces marxianus b0399 in fermented milk with added sorbic acid. | in this study, the survival of the functional yeast kluyveromyces marxianus b0399 in an industrially produced fermented milk was evaluated. in particular, the yeast viability was assessed throughout the entire shelf-life of the product (30 d) to ensure the presence of the effective yeast dose (20 million viable cells for each serving of 125 g) while avoiding, by sorbic acid addition, yeast growth, which could affect product quality and stability. to find the best combination of yeast and sorbic ... | 2016 | 26547644 |
| data for rapid ethanol production at elevated temperatures by engineered thermotolerant kluyveromyces marxianus via the nadp(h)-preferring xylose reductase-xylitol dehydrogenase pathway. | a thermo-tolerant nadp(h)-preferring xylose pathway was constructed in kluyveromyces marxianus for ethanol production with xylose at elevated temperatures (zhang et al., 2015 [25]). ethanol production yield and efficiency was enhanced by pathway engineering in the engineered strains. the constructed strain, yzj088, has the ability to co-ferment glucose and xylose for ethanol and xylitol production, which is a critical step toward enabling economic biofuel production from lignocellulosic biomass. ... | 2015 | 26543879 |
| dynamics of yeast immobilized-cell fluidized-bed bioreactors systems in ethanol fermentation from lactose-hydrolyzed whey and whey permeate. | we studied the dynamics of ethanol production on lactose-hydrolyzed whey (lhw) and lactose-hydrolyzed whey permeate (lhwp) in batch fluidized-bed bioreactors using single and co-cultures of immobilized cells of industrial strains of saccharomyces cerevisiae and non-industrial strains of kluyveromyces marxianus. although the co-culture of s. cerevisiae cat-1 and k. marxianus cct 4086 produced two- to fourfold the ethanol productivity of single cultures of s. cerevisiae, the single cultures of the ... | 2016 | 26527573 |
| kefir-isolated bacteria and yeasts inhibit shigella flexneri invasion and modulate pro-inflammatory response on intestinal epithelial cells. | the aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (mm) to protect intestinal epithelial cells against shigella flexneri invasion, as well as to analyse the effect on pro-inflammatory response elicited by this pathogen. a significant decrease in s. flexneri strain 72 invasion was observed on both ht-29 and caco-2 cells pre-incubated with mm. pre-incubation with the individual strains saccharomyces cerevisiae cid ... | 2016 | 26503738 |
| cloning, expression, characterization, and mutagenesis of a thermostable exoinulinase from kluyveromyces cicerisporus. | inulinase is an enzyme that belongs to glycoside hydrolase family 32. it converts inulin into high-fructose syrups and fructoligosaccharides, both of which are widely used in pharmaceutical and food industries. in this study, the kcinu1 gene (genbank accession number af178979) encoding an exoinulinase was cloned from kluyveromyces cicerisporus cbs4857 and expressed in pichia pastoris x-33, yielding a maximum of 45.2 ± 0.6 u ml(-1) of inulinase activity of culture supernatant. the expressed inuli ... | 2016 | 26446826 |
| chromosome arrangement, differentiation of growth kinetics and volatile molecule profiles in kluyveromyces marxianus strains from italian cheeses. | thirty-nine strains of kluyveromyces marxianus from pecorino di farindola cheese in comparison with 3 strains from parmigiano reggiano cheese, 1 from fermented milk, 3 from cow whey and two type strains k. marxianus cbs 834(t) and kluyveromyces lactis cbs 683(t) were tested for genetic and metabolic characteristics. intraspecific diversity of chromosome arrangements was evaluated by pulsed field gel electrophoresis (pfge) analysis. among k. marxianus strains chromosome polymorphisms were evident ... | 2015 | 26310129 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| transcriptional analysis of kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology. | ethanol production from non-crop materials, such as jerusalem artichokes, would make a great contribution to the energy industry. the non-conventional yeast, kluyveromyces marxianus, is able to carry out ethanol fermentation of sugar molecules obtained from inulin-containing materials by consolidated bioprocessing. lower inulin concentrations and micro-aeration can lead to a relatively fast and ideal fermentation process; however, it is unclear what causes the inhibition of higher concentrations ... | 2015 | 26273319 |
| hypocholesterolemic effects of kluyveromyces marxianus m3 isolated from tibetan mushrooms on diet-induced hypercholesterolemia in rat. | to investigate the effects of kluyveromyces marxianus m3 isolated from tibetan mushrooms on diet-induced hypercholesterolemia in rats, female wistar rats were fed a high-cholesterol diet (hcd) for 28 d to generate hyperlipidemic models. hyperlipidemic rats were assigned to four groups, which were individually treated with three different dosages of k. marxianus m3+hcd or physiological saline+hcd via oral gavage for 28 d. the total cholesterol (tc), triglycerides (tg), high-density lipoprotein ch ... | 2015 | 26273253 |
| yeast kluyveromyces lactis as host for expression of the bacterial lipase: cloning and adaptation of the new lipase gene from serratia sp. | many microbial lipases have been successfully expressed in yeasts, but not in industrially attractive kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey--cheap and easily available industrial waste. a new bacterial lipase from serratia sp. was isolated and for the first time expressed into the yeast kluyveromyces lactis by heterologous protein expression system based on a strong promoter of kluyveromyces marxianus triosephosphate isomerase gene ... | 2015 | 26254038 |
| rapid ethanol production at elevated temperatures by engineered thermotolerant kluyveromyces marxianus via the nadp(h)-preferring xylose reductase-xylitol dehydrogenase pathway. | conversion of xylose to ethanol by yeasts is a challenge because of the redox imbalances under oxygen-limited conditions. the thermotolerant yeast kluyveromyces marxianus grows well with xylose as a carbon source at elevated temperatures, but its xylose fermentation ability is weak. in this study, a combination of the nadph-preferring xylose reductase (xr) from neurospora crassa and the nadp(+)-preferring xylitol dehydrogenase (xdh) mutant from scheffersomyces stipitis (pichia stipitis) was cons ... | 2015 | 26253204 |
| the modeling of ethanol production by kluyveromyces marxianus using whey as substrate in continuous a-stat bioreactors. | we investigated the kinetics of whey bioconversion into ethanol by kluyveromyces marxianus in continuous bioreactors using the "accelerostat technique" (a-stat). cultivations using free and ca-alginate immobilized cells were evaluated using two different acceleration rates (a). the kinetic profiles of these systems were modeled using four different unstructured models, differing in the expressions for the specific growth (μ) and substrate consumption rates (r s), taking into account substrate li ... | 2015 | 26233317 |
| characterizing yeast promoters used in kluyveromyces marxianus. | fermentation at higher temperatures can potentially reduce the cooling cost in large-scale fermentation and reduce the contamination risk. thus, the thermotolerant yeast, kluyveromyces marxianus, which can grow and ferment at elevated temperatures, is a promising biotechnological tool for future applications. however, the promoters used in k. marxianus are not well characterized, especially at elevated temperatures, which is important in efficient metabolic pathway construction. in this study, s ... | 2015 | 26164057 |
| gene expression analysis using strains constructed by nhej-mediated one-step promoter cloning in the yeast kluyveromyces marxianus. | gene expression analysis provides valuable information to evaluate cellular state. unlike quantitative mrna analysis techniques like reverse-transcription pcr and microarray, expression analysis using a reporter gene has not been commonly used for multiple-gene analysis, probably due to the difficulty in preparing multiple reporter-gene constructs. to circumvent this problem, we developed a novel one-step reporter-gene construction system mediated by non-homologous end joining (nhej) in the yeas ... | 2015 | 26136515 |
| novel transporters from kluyveromyces marxianus and pichia guilliermondii expressed in saccharomyces cerevisiae enable growth on l-arabinose and d-xylose. | genes encoding l-arabinose transporters in kluyveromyces marxianus and pichia guilliermondii were identified by functional complementation of saccharomyces cerevisiae whose growth on l-arabinose was dependent on a functioning l-arabinose transporter, or by screening a differential display library, respectively. these transporters also transport d-xylose and were designated kmaxt1 (arabinose-xylose transporter) and pgaxt1, respectively. transport assays using l-arabinose showed that kmaxt1p has k ... | 2015 | 26129747 |
| looking beyond saccharomyces: the potential of non-conventional yeast species for desirable traits in bioethanol fermentation. | saccharomyces cerevisiae has been used for millennia in the production of food and beverages and is by far the most studied yeast species. currently, it is also the most used microorganism in the production of first-generation bioethanol from sugar or starch crops. second-generation bioethanol, on the other hand, is produced from lignocellulosic feedstocks that are pretreated and hydrolyzed to obtain monomeric sugars, mainly d-glucose, d-xylose and l-arabinose. recently, s. cerevisiae recombinan ... | 2015 | 26126524 |
| cell lysis induced by membrane-damaging detergent saponins from quillaja saponaria. | this paper presents the results of a study to determine the effect of quillaja saponaria saponins on the lysis of industrial yeast strains. cell lysis induced by saponin from q. saponaria combined with the plasmolysing effect of 5% nacl for saccharomyces cerevisiae, kluyveromyces marxianus yeasts biomass was conducted at 50 °c for 24-48 h. membrane permeability and integrity of the yeast cells were monitored using fluorescent techniques and concentrations of proteins, free amino nitrogen (fan) a ... | 2015 | 26047915 |
| enhanced xylitol production by mutant kluyveromyces marxianus 36907-fmel1 due to improved xylose reductase activity. | a directed evolution and random mutagenesis were carried out with thermotolerant yeast kluyveromyces marxianus atcc 36907 for efficient xylitol production. the final selected strain, k. marxianus 36907-fmel1, exhibited 120 and 39 % improvements of xylitol concentration and xylitol yield, respectively, as compared to the parental strain, k. marxianus atcc 36907. according to enzymatic assays for xylose reductase (xr) activities, xr activity from k. marxianus 36907-fmel1 was around twofold higher ... | 2015 | 26043853 |
| neuro-fuzzy based model of batch fermentation of kluyveromyces marxianus var. lactis mc5. | in this work a neuro-fuzzy based model of a whey batch fermentation process by a strain kluyveromyces marxianus var. lactis mc5 is presented. a three-layered neuro-fuzzy network is realized. the simulation results are compared with conventional models (based on mass balance and differential equations). the neuro-fuzzy model provides a better fitness and allows inclusion of linguistic variables (such as colour, smell, taste, morphophysiology, etc.). the accuracy is approximately equal to this ach ... | 2014 | 26019585 |
| production of lactulose oligosaccharides by isomerisation of transgalactosylated cheese whey permeate obtained by β-galactosidases from dairy kluyveromyces. | β-galactosidases from kluyveromyces lactis and kluyveromyces marxianus isolated from artisanal ewes' milk cheeses, were used to transgalactosylate lactose from cheese whey permeate (wp). the content of galactooligosaccharides (gos) obtained by transgalactosylation was comparable with that formed using pure lactose as substrate. in order to obtain a mixture with higher prebiotic oligosaccharide content, isomerisation of the transgalactosylated wp was carried out using sodium aluminate as catalyst ... | 2015 | 26004434 |
| ethanol production from jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast kluyveromyces marxianus using consolidated bioprocessing. | thermotolerant inulin-utilizing yeast strains were successfully isolated in this study. among the isolated strains, kluyveromyces marxianus dbkku y-102 was found to be the most effective strain for direct ethanol fermentation at high temperature from fresh jerusalem artichoke (ja) tubers without inulin hydrolysis under consolidated bioprocessing (cbp). the maximum ethanol concentrations produced by this strain under the optimum culture conditions were 104.83 and 97.46 g l(-1) at 37 and 40 °c, re ... | 2015 | 25980834 |
| transgalactosylation of lactose for synthesis of galacto-oligosaccharides using kluyveromyces marxianus ncim 3551. | among a number of yeast strains screened for whole cell transgalactosylating activity, kluyveromyces marxianus ncim 3551 was found to be most suitable biocatalyst for production of galacto-oligosaccharides (gos). cell permeabilization lead to an efficient bioconversion by β-galactosidase resulting in synthesis of gos. a maximum gos yield of 36% (w/w) of total sugars was achieved and the products consisted of tri- and tetra-galacto-oligosaccharides. a lactose conversion rate of 80% and productivi ... | 2015 | 25976627 |
| [expression of inulinase genes in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus]. | expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus was studied. the exon-intron structure of the relevant genes was identified and the primers for quantitative pcr were optimized. expression of the genes was found to depend on the carbon source. glucose was shown to exhibit a repressive effect on inulinase synthesis by k. marxianus, while in s. cerevisiae glucose and sucrose were inul ... | 2015 | 25916145 |
| complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast. | we determined the genome sequence of the thermotolerant yeast kluyveromyces marxianus strain nbrc1777. the genome of strain nbrc1777 is composed of 4,912 open reading frames (orfs) on 8 chromosomes, with a total size of 10,895,581 bp, including mitochondrial dna. | 2015 | 25908152 |
| synthetic signal sequences that enable efficient secretory protein production in the yeast kluyveromyces marxianus. | targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the n-terminus of a secretory protein. these signal sequences usually contain an n-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signal sequence has been identified. in this study, simple modeling of signal sequences was attempted using gaussia princeps secretory luciferase (gluc) in the yeast kluyveromyces marxianus, which ... | 2015 | 25889890 |
| genetic basis of the highly efficient yeast kluyveromyces marxianus: complete genome sequence and transcriptome analyses. | high-temperature fermentation technology with thermotolerant microbes has been expected to reduce the cost of bioconversion of cellulosic biomass to fuels or chemicals. thermotolerant kluyveromyces marxianus possesses intrinsic abilities to ferment and assimilate a wide variety of substrates including xylose and to efficiently produce proteins. these capabilities have been found to exceed those of the traditional ethanol producer saccharomyces cerevisiae or lignocellulose-bioconvertible ethanolo ... | 2015 | 25834639 |
| comparison of a pectinolytic extract of kluyveromyces marxianus and a commercial enzyme preparation in the production of ives (vitis labrusca) grape juice. | this study analyses the effect of the crude enzymatic extract produced by kluyveromyces marxianus (eeb) in the maceration and clarification of juice produced from ives (vitis labrusca) grapes compared to the commercial enzyme preparation pectinex(®)ultra color (pec). treatments were conducted with a total pectinolytic activity of 1 u/ml of fruit juice, at 40 °c, for 60 min. after the enzymatic treatment, the juices were evaluated with respect to yield, viscosity, and degree of clarification, as ... | 2015 | 25721421 |
| [investigation of mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of ion-exchange resins and fiber]. | it is established that ion exchange resins av-17-2p, ku-2, av-16-gs, am 21a, imac-hp, purolite and fiber vion kn-1 can be applied as carriers for inulinase immobilization. the analysis of ir spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through electrostatic interactions. it is assumed that the mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of cation- ... | 2015 | 25702479 |
| enhanced enzymatic hydrolysis and ethanol production from cashew apple bagasse pretreated with alkaline hydrogen peroxide. | the effect of combinations and ratios between different enzymes has been investigated in order to assess the optimal conditions for hydrolysis of cashew apple bagasse pretreated with alkaline hydrogen peroxide (the solids named cab-ahp). the separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes were evaluated in the ethanol production. the enzymatic hydrolysis conducted with cellulase complex and β-glucosidase in a ratio of 0.61:0.39, enzym ... | 2015 | 25545094 |
| studies on the mechanism of synthesis of ethyl acetate in kluyveromyces marxianus dsm 5422. | kluyveromyces marxianus converts whey-borne sugar into ethyl acetate, an environmentally friendly solvent with many applications. k. marxianus dsm 5422 presumably synthesizes ethyl acetate from acetyl-scoa. iron limitation as a trigger for this synthesis is explained by a diminished aconitase and succinate dehydrogenase activity (both enzymes depend on iron) causing diversion of acetyl-scoa from the tricarboxic acid cycle to ester synthesis. copper limitation as another trigger for ester synthes ... | 2015 | 25487884 |
| whole cells in enantioselective reduction of benzyl acetoacetate. | the β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (s)-3-hydroxybutanoate by seven microorganism species. the best result using free cells was obtained with the yeast hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. after immobilization in calcium alginate spheres, k.marxianus showed to be more stable after 2 cycles of reaction. | 2014 | 25477927 |
| isotope labeling pattern study of central carbon metabolites using gc/ms. | determination of fluxes by (13)c tracer experiments depends on monitoring the (13)c labeling pattern of metabolites during isotope experiments. in metabolome-based (13)c metabolic flux analysis, liquid chromatography combined with mass spectrometry or tandem mass spectrometry (lc/ms or lc/ms/ms, respectively) has been mainly used as an analytical platform for isotope pattern studies of central carbon metabolites. however, gas chromatography with mass spectrometry (gc/ms) has several advantages o ... | 2015 | 25463204 |
| cell factory applications of the yeast kluyveromyces marxianus for the biotechnological production of natural flavour and fragrance molecules. | kluyveromyces marxianus is emerging as a new platform organism for the production of flavour and fragrance (f&f) compounds. this food-grade yeast has advantageous traits, such as thermotolerance and rapid growth, that make it attractive for cell factory applications. the major impediment to its development has been limited fundamental knowledge of its genetics and physiology, but this is rapidly changing. k. marxianus produces a wide array of volatile molecules and contributes to the flavour of ... | 2015 | 25393382 |
| genomic sequence of the yeast kluyveromyces marxianus cct 7735 (ufv-3), a highly lactose-fermenting yeast isolated from the brazilian dairy industry. | here, we present the draft genome sequence of kluyveromyces marxianus cct 7735 (ufv-3), including the eight chromosomes and the mitochondrial genomic sequences. | 2014 | 25377712 |
| protein enrichment of an opuntia ficus-indica cladode hydrolysate by cultivation of candida utilis and kluyveromyces marxianus. | the cladodes of opuntia ficus-indica (prickly pear cactus) have a low protein content; for use as a balanced feed, supplementation with other protein sources is therefore desirable. we investigated protein enrichment by cultivation of the yeasts candida utilis and kluyveromyces marxianus in an enzymatic hydrolysate of the cladode biomass. | 2015 | 25371280 |
| influence of carbon and nitrogen source on production of volatile fragrance and flavour metabolites by the yeast kluyveromyces marxianus. | the yeast kluyveromyces marxianus produces a range of volatile molecules with applications as fragrances or flavours. the purpose of this study was to establish how nutritional conditions influence the production of these metabolites. four strains were grown on synthetic media, using a variety of carbon and nitrogen sources and volatile metabolites analysed using gas chromatography-mass spectrometry (gc-ms). the nitrogen source had pronounced effects on metabolite production: levels of the fusel ... | 2015 | 25308412 |
| growth and by-product profiles of kluyveromyces marxianus cells immobilized in foamed alginate. | the aim of this research was to study how the yeast cell immobilization technique influences the growth and fermentation profiles of kluyveromyces marxianus cultivated on apple/chokeberry and apple/cranberry pomaces. encapsulation of the cells was performed by droplet formation from a foamed alginate solution. the growth and metabolic profiles were evaluated for both free and immobilized cells. culture media with fruit waste produced good growth of free as well as immobilized yeast cells. the fe ... | 2015 | 25277269 |
| opuntia ficus-indica cladodes as feedstock for ethanol production by kluyveromyces marxianus and saccharomyces cerevisiae. | the feasibility of ethanol production using an enzymatic hydrolysate of pretreated cladodes of opuntia ficus-indica (prickly pear cactus) as carbohydrate feedstock was investigated, including a comprehensive chemical analysis of the cladode biomass and the effects of limited aeration on the fermentation profiles and sugar utilization. the low xylose and negligible mannose content of the cladode biomass used in this study suggested that the hemicellulose structure of the o. ficus-indica cladode w ... | 2014 | 25248867 |
| sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept. | the environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. in coffee-producing countries, improved technology for treat ... | 2014 | 25204861 |
| screening of accurate clones for gene synthesis in yeast. | methods for error-less gene synthesis are desired because synthesized genes often contain mutations. by cloning pcr-assembled oligonucleotide fragments fused to a selection marker in yeast, we developed a novel method to screen accurate clones in gene synthesis. as a model case, the 555-bp luciferase gene from gaussia princeps (gluc) was synthesized to contain yeast-optimized codons (called ygluc hereafter). after standard pcr-mediated oligonucleotide assembly, many clones showed no luciferase a ... | 2015 | 25201012 |
| analysis of the yeast short-term crabtree effect and its origin. | the short-term crabtree effect is defined as the immediate occurrence of aerobic alcoholic fermentation in response to provision of a pulse of excess sugar to sugar-limited yeast cultures. here we have characterized ten yeast species with a clearly defined phylogenetic relationship. yeast species were cultivated under glucose-limited conditions, and we studied their general carbon metabolism in response to a glucose pulse. we generated an extensive collection of data on glucose and oxygen consum ... | 2014 | 25161062 |
| optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant saccharomyces cerevisiae carrying kluyveromyces marxianus inulinase. | inulin is a polyfructan that is abundant in plants such as jerusalem artichoke, chicory and dahlia. inulinase can easily hydrolyze inulin to fructose, which is consumed by microorganisms. generally, saccharomyces cerevisiae, an industrial workhorse strain for bioethanol production, is known for not having inulinase activity. the inulinase gene from kluyveromyces marxianus (kminu), with the ability of converting inulin to fructose, was introduced into s. cerevisiae d452-2. the inulinase gene was ... | 2015 | 25142154 |
| potential application of aqueous two-phase systems and three-phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of kluyveromyces marxianus. | superoxide dismutase (sod; ec 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. in the present work, the partition behavior of sod in aqueous two-phase systems (atps) (using a standard solution and a complex extract from kluyveromyces marxianus as sample) was characterized on different types of atps (polymer-polymer, polymer-salt, alcohol-salt, and ionic liquid (il)- ... | 2016 | 25138773 |
| draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate. | we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes. | 2014 | 25059876 |
| steam explosion treatment for ethanol production from branches pruned from pear trees by simultaneous saccharification and fermentation. | this study investigated the production of ethanol from unutilized branches pruned from pear trees by steam explosion pretreatment. steam pressures of 25, 35, and 45 atm were applied for 5 min, followed by enzymatic saccharification of the extracted residues with cellulase (cellic ctec2). high glucose recoveries, of 93.3, 99.7, and 87.1%, of the total sugar derived from the cellulose were obtained from water- and methanol-extracted residues after steam explosion at 25, 35, and 45 tm, respectively ... | 2014 | 25036499 |
| fine structure of tibetan kefir grains and their yeast distribution, diversity, and shift. | tibetan kefir grains (tkgs), a kind of natural starter for fermented milk in tibet, china, host various microorganisms of lactic acid bacteria, yeasts, and occasionally acetic acid bacteria in a polysaccharide/protein matrix. in the present study, the fine structure of tkgs was studied to shed light on this unusual symbiosis with stereomicroscopy and thin sections. the results reveal that tkgs consist of numerous small grain units, which are characterized by a hollow globular structure with a di ... | 2014 | 24977409 |
| deletion of a ku80 homolog enhances homologous recombination in the thermotolerant yeast kluyveromyces marxianus. | targeted gene replacement in the thermotolerant yeast kluyveromyces marxianus kctc 17555 has been hampered by its propensity to non-homologous end joining (nhej). to enhance homologous recombination (hr) by blocking nhej, we identified and disrupted the k. marxianus ku80 gene. the ku80 deletion mutant strain (kmku80∆) of k. marxianus kctc 17555 did not show apparent growth defects under several conditions with the exception of exposure to tunicamycin. the targeted disruption of the three model g ... | 2014 | 24930110 |
| sulfuric acid hydrolysis and detoxification of red alga pterocladiella capillacea for bioethanol fermentation with thermotolerant yeast kluyveromyces marxianus. | one-step sulfuric acid saccharification of the red alga pterocladiella capillacea was optimized, and various detoxification methods (neutralization, overliming, and electrodialysis) of the acid hydrolysate were evaluated for fermentation with the thermotolerant yeast kluyveromyces marxianus. a proximate composition analysis indicated that p. capillacea was rich in carbohydrates. a significant galactose recovery of 81.1 ± 5% was also achieved under the conditions of a 12% (w/v) biomass load, 5% ( ... | 2014 | 24851812 |
| mixed culture of kluyveromyces marxianus and candida krusei for single-cell protein production and organic load removal from whey. | the study was conducted to evaluate the potential of mixed culture of kluyveromyces marxianus and candida krusei to enhance cod removal efficiency, minimize contamination at extreme conditions (high temperature 40°c and low ph 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (scp) using whey as substrate. the batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher cod removal efficac ... | 2014 | 24844166 |
| whey fermentation by yeast strains kluyveromyces marxianus ucm y-2096 and ucm y-2388. | two kluyveromyces marxianus strains have been tested for their ability to ferment lactose in the medium containing cheese whey powder (cwp) and lactose at different concentrations. the ethanol production by the selected strains was inhibited by the increase in substrate concentration (100 g/l). the strain k. marxianus ucm y-2096 was found to yield the higher ethanol formation and to be more resistant to ethanol under microaerophilic conditions. | 2015 | 24800512 |
| ethanol production from sunflower meal biomass by simultaneous saccharification and fermentation (ssf) with kluyveromyces marxianus atcc 36907. | the lignocellulosic materials are considered promising renewable resources for ethanol production, but improvements in the processes should be studied to reduce operating costs. thus, the appropriate enzyme loading for cellulose saccharification is critical for process economics. this study aimed at evaluating the concentration of cellulase and β-glucosidase in the production of bioethanol by simultaneous saccharification and fermentation (ssf) of sunflower meal biomass. the sunflower biomass wa ... | 2014 | 24794173 |
| perspectives for the biotechnological production of ethyl acetate by yeasts. | ethyl acetate is an environmentally friendly solvent with many industrial applications. the production of ethyl acetate currently proceeds by energy-intensive petrochemical processes which are based on natural gas and crude oil without exception. microbial synthesis of ethyl acetate could become an interesting alternative. the formation of esters as aroma compounds in food has been repeatedly reviewed, but a survey which deals with microbial synthesis of ethyl acetate as a bulk product is missin ... | 2014 | 24788328 |
| development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste. | this work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. cheese whey was used in kluyveromyces marxianus and lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. the production o ... | 2014 | 24785788 |
| growth inhibition of thermotolerant yeast, kluyveromyces marxianus, in hydrolysates from cassava pulp. | in this study, we report the inhibition of kluyveromyces marxianus tistr5925 growth and ethanol fermentation in the presence of furan derivatives and weak acids (acetic acid and lactic acid) at high temperatures. cassava pulp, obtained as the waste from starch processing, was collected from 14 starch factories located in several provinces of thailand. at a high temperature (42 °c), the cassava pulp hydrolysate from some starch factories strongly inhibited growth and ethanol production of both k. ... | 2014 | 24781978 |
| a biosynthetic pathway for hexanoic acid production in kluyveromyces marxianus. | hexanoic acid can be used for diverse industrial applications and is a precursor for fine chemistry. although some natural microorganisms have been screened and evolved to produce hexanoic acid, the construction of an engineered biosynthetic pathway for producing hexanoic acid in yeast has not been reported. here we constructed hexanoic acid pathways in kluyveromyces marxianus by integrating 5 combinations of seven genes (atob, bktb, crt, hbd, mct1, ter, and tes1), by which random chromosomal si ... | 2014 | 24768798 |
| effect of oxygenation and temperature on glucose-xylose fermentation in kluyveromyces marxianus cbs712 strain. | the yeast kluyveromyces marxianus features specific traits that render it attractive for industrial applications. these include production of ethanol which, together with thermotolerance and the ability to grow with a high specific growth rate on a wide range of substrates, could make it an alternative to saccharomyces cerevisiae as an ethanol producer. however, its ability to co-ferment c5 and c6 sugars under oxygen-limited conditions is far from being fully characterized. | 2014 | 24712908 |
| industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass. | the search of robust microorganisms is essential to design sustainable processes of second generation bioethanol. yeast strains isolated from industrial environments are generally recognised to present an increased stress tolerance but no specific information is available on their tolerance towards inhibitors that come from the pretreatment of lignocellulosic materials. in this work, a strategy for the selection of different yeasts using hydrothermal hydrolysate from eucalyptus globulus wood, co ... | 2014 | 24704884 |
| interactions between kluyveromyces marxianus and saccharomyces cerevisiae in tequila must type medium fermentation. | traditional tequila fermentation is a complex microbial process performed by different indigenous yeast species. usually, they are classified in two families: saccharomyces and non-saccharomyces species. using mixed starter cultures of several yeasts genera and species is nowadays considered to be beneficial to enhance the sensorial characteristics of the final products (taste, odor). however, microbial interactions occurring in such fermentations need to be better understood to improve the proc ... | 2014 | 24677041 |
| potential of some yeast strains in the stereoselective synthesis of (r)-(-)-phenylacetylcarbinol and (s)-(+)-phenylacetylcarbinol and their reduced 1,2-dialcohol derivatives. | whole cells of different yeast species have been widely used for a number of asymmetric transformations. in the present study, the screening of several yeast strains revealed the utility of debaryomyces etchellsii in acyloin condensation for (r)-(-)-phenylacetylcarbinol production. some conditions for the efficient biotransformation of benzaldehyde and minimization in the production of by-products were explored: ph of the reaction medium, use of additives (ethanol or acetonitrile), temperature, ... | 2014 | 24671162 |
| modeling the microbial growth and temperature profile in a fixed-bed bioreactor. | aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ... | 2014 | 24658796 |
| biosorption of lead by kluyveromyces marxianus immobilized in alginate beads. | the uptake and recovery of pb (ii) ions were investigated by using sodium alginate beads. biosorption experiments are carried out in batch mode. the experimental results showed that the beads were effective in removing pb (ii) ions from solution. biosorption equilibrium was approached within 16hrs. pseudo first order was applicable to all the sorption data over the entire time range. the sorption data conformed well for the langmuir isotherm model. the maximum adsorption capacity (qmax) onto alg ... | 2013 | 24558794 |
| physiological characterization of thermotolerant yeast for cellulosic ethanol production. | the conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (ssf). in this study, a comparison was performed between the yeast species saccharomyces cerevisiae and kluyveromyces marxianus for their potential use in ssf process. three strains of s. cerevisiae were evaluated: two are widely used in the brazilian etha ... | 2014 | 24535257 |
| hydrolysis of agave fourcroydes lemaire (henequen) leaf juice and fermentation with kluyveromyces marxianus for ethanol production. | carbon sources for biofuel production are wide-ranging and their availability depends on the climate and soil conditions of the land where the production chain is located. henequen (agave fourcroydes lem.) is cultivated in yucatán, mexico to produce natural fibers from the leaves, and a juice containing fructans is produced during this process. fructans can be hydrolyzed to fructose and glucose and metabolized into ethanol by appropriate yeasts. in mexico, different agave species provide the car ... | 2014 | 24529165 |
| a broad-range yeast expression system reveals arxula adeninivorans expressing a fungal self-sufficient cytochrome p450 monooxygenase as an excellent whole-cell biocatalyst. | the feasibility of using a single vector to clone a cytochrome p450 monooxygenase (p450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. a broad-range yeast expression vector using the yltefp to drive expression of the cloned gene and the sctefp to drive the hygromycin resistance marker gene was used to clone the genes encoding two self-sufficient p450s, cyp102a1 and cyp505a1. both genes were cloned into saccharomyces cerevisiae, kluyveromyces marxianus, ya ... | 2014 | 24521080 |
| permeabilization of kluyveromyces marxianus with mild detergent for whey lactose hydrolysis and augmentation of mixed culture. | cheese whey is a by-product of cheese-manufacturing industries, and the utilization of whey is a challenging problem either to use it or dispose it, because only few microorganisms can metabolize the whey lactose. enzymatic hydrolysis of whey lactose to glucose and galactose by β-galactosidase is the approach for biotechnological application. kluyveromyces marxianus cells were permeabilized with non-toxic, biodegradable, anionic detergent n-lauroyl sarcosine (n-ls) for the enzyme activity. the p ... | 2014 | 24500798 |
| novel antihypertensive lactoferrin-derived peptides produced by kluyveromyces marxianus: gastrointestinal stability profile and in vivo angiotensin i-converting enzyme (ace) inhibition. | novel antihypertensive peptides released by kluyveromyces marxianus from bovine lactoferrin (lf) have been identified. k. marxianus lf permeate was fractionated by semipreparative high performance liquid chromatography and 35 peptides contained in the angiotensin i-converting enzyme (ace)-inhibitory fractions were identified by using an ion trap mass spectrometer. on the basis of peptide abundance and common structural features, six peptides were chemically synthesized. four of them (dpyklrp, py ... | 2014 | 24476136 |
| fermentative production of ribonucleotides from whey by kluyveromyces marxianus: effect of temperature and ph. | ribonucleotides have shown many promising applications in food and pharmaceutical industries. the aim of the present study was to produce ribonucleotides (rna) by kluyveromyces marxianus atcc 8,554 utilizing cheese whey, a dairy industry waste, as a main substrate under batch fermentation conditions. the effects of temperature, ph, aeration rate, agitation and initial cellular concentration were studied simultaneously through factorial design for rna, biomass production and lactose consumption. ... | 2013 | 24426003 |
| production and characterization of β-glucanase secreted by the yeast kluyveromyces marxianus. | an extracellular β-glucanase secreted by kluyveromyces marxianus was identified for the first time. the optimal conditions for the production of this enzyme were evaluated by response surface methodology. the optimal conditions to produce β-glucanase were a glucose concentration of 4% (w/v), a ph of 5.5, and an incubation temperature of 35 °c. response surface methodology was also used to determine the ph and temperature required for the optimal enzymatic activity. the highest enzyme activity wa ... | 2014 | 24390854 |
| variation in phenolic compounds, anthocyanins, and color in red wine treated with enzymatic extract of kluyveromyces marxianus. | the effect of the addition of enzymatic extract of kluyveromyces marxianus nrrl-y-7571 during the maceration and fermentation steps of cabernet sauvignon wine production was evaluated. the results obtained in the analytical determinations of the wines showed levels within the limits established by legislation and similar to values found in other studies. the results show that by adding the enzyme to the red wines these showed color characteristics considered to be superior to those of the contro ... | 2014 | 24347085 |
| xylitol production at high temperature by engineered kluyveromyces marxianus. | several recombinant kluyveromyces marxianus strains were constructed through overexpressing the neurospora crassa xylose reductase genes. yzj015, which maintained the original xylitol dehydrogenase gene, produced xylitol with the highest productivity (1.49 g l(-1) h(-1)) from 100 g l(-1) xylose at 42 °c. even at 45 °c, yzj015 was still able to produce 60.03 g l(-1) xylitol from 100 g l(-1) xylose with a productivity of 1.25 g l(-1)h(-1). in addition, for 20 rounds of cell recycling at 42 °c, yzj ... | 2014 | 24291795 |
| optimization, kinetics, and modeling of inulinase production by k. marxianus var. marxianus. | pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (ssf). statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by kluyveromyces marxianus var. marxianus. eighteen various nutrients were selected for preliminary screening of production medium component by plackett-burman design (pbd) technique. five nutrients were found to be ... | 2014 | 24274017 |
| selection of a thermotolerant kluyveromyces marxianus strain with potential application for cellulosic ethanol production by simultaneous saccharification and fermentation. | the development of technologies for cellulosic ethanol production by simultaneous saccharification and fermentation (ssf) depends on the use of microorganisms with high fermentative rates and thermotolerance. in this study, the ability of five kluyveromyces marxianus strains to produce ethanol from glucose at 45 °c was investigated. the highest fermentative parameters were observed with k. marxianus nrrl y-6860, which was then further studied. an initial evaluation of the oxygen supply on ethano ... | 2014 | 24222495 |
| simultaneous single-cell protein production and cod removal with characterization of residual protein and intermediate metabolites during whey fermentation by k. marxianus. | cheese whey fermentation with kluyveromyces marxianus was carried out at 40 °c and ph 3.5 to examine simultaneous single-cell protein production and chemical oxygen demand (cod) removal, determine the fate of soluble whey protein and characterize intermediate metabolites. after 36 h of batch fermentation, the biomass concentration increased from 2.0 to 6.0 g/l with 55 % cod reduction (including protein), whereas soluble whey protein concentration decreased from 5.6 to 4.1 g/l. it was confirmed t ... | 2014 | 24185705 |
| identification and assessment of kefir yeast potential for sugar/ethanol-resistance. | biochemical and molecular analysis was used for identification of different kefir yeasts species from brazil, canada and the united states of america. the sugar/ethanol-resistant activity of the yeasts was evaluated. saccharomyces cerevisiae and kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains. | 2013 | 24159292 |
| identification of auxotrophic mutants of the yeast kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from saccharomyces cerevisiae. | the isolation and application of auxotrophic mutants for gene manipulations, such as genetic transformation, mating selection and tetrad analysis, form the basis of yeast genetics. for the development of these genetic methods in the thermotolerant fermentative yeast kluyveromyces marxianus, we isolated a series of auxotrophic mutants with defects in amino acid or nucleic acid metabolism. to identify the mutated genes, linear dna fragments of nutrient biosynthetic pathway genes were amplified fro ... | 2013 | 24150815 |
| effects of signal sequences and folding accessory proteins on extracellular expression of carboxypeptidase y in recombinant saccharomyces cerevisiae. | carboxypeptidase y (cpy) is a yeast vacuolar protease with useful applications including c-terminal sequencing of peptides and terminal modification of target proteins. to overexpress cpy with the pro-sequence (procpy) encoded by the saccharomyces cerevisiae prc1 gene in recombinant s. cerevisiae, the procpy gene was combined with the gene coding for a signal sequence of s. cerevisiae mating factor α (mfα), invertase (suc2), or kluyveromyces marxianus inulinase (inu1). among the three constructs ... | 2014 | 24146283 |
| solid state fermentation of food waste mixtures for single cell protein, aroma volatiles and fat production. | growth of selected microorganisms of industrial interest (saccharomyces cerevisiae, kluyveromyces marxianus and kefir) by solid state fermentation (ssf) of various food industry waste mixtures was studied. the fermented products were analysed for protein, and nutrient minerals content, as well as for aroma volatile compounds by gc/ms. the substrate fermented by k. marxianus contained the highest sum of fat and protein concentration (59.2% w/w dm) and therefore it could be considered for utilisat ... | 2014 | 24128535 |
| albariño wine aroma enhancement through the use of a recombinant polygalacturonase from kluyveromyces marxianus. | the possible biotechnological application of a recombinant endopolygalacturonase of kluyveromyces marxianus (kmpg) for the aroma enhancement of albariño wine was studied. the addition of this enzyme to the must gives rise to a significant increase of the total compounds responsible for the aroma as opposed to the effect when using a commercial pectic enzyme. this increase also results in a significant rise of the odoriferous aglycones which are direct determinants of the aroma. wines made by usi ... | 2014 | 24128465 |
| growth of kluyveromyces marxianus and formation of ethyl acetate depending on temperature. | conversion of lactose into ethyl acetate by kluyveromyces marxianus allows economic reuse of whey-borne sugar. the high volatility of ethyl acetate enables its process-integrated recovery by stripping. this stripping is governed by both the aeration rate and the partition coefficient, k ea,l/g. cultivation at elevated temperatures should decrease the k ea,l/g value and thus favor stripping. k. marxianus dsm 5422 as a potent producer of ethyl acetate was cultivated aerobically in whey-borne media ... | 2013 | 24104467 |
| comparison of ethanol production from cheese whey permeate by two yeast strains. | the objective of the present laboratory scale experiment was to compare ethanol production by kluyveromyces marxianus strain atcc8554 and candida kefyr atcc 14245 from unconcentrated and concentrated cheese whey permeate. the results indicated that ethanol production was greater when using concentrated whey permeate (9.8% lactose) compared to unconcentrated whey permeate (4.9% lactose) by both the yeasts, especially in presence of growth supplements. the rate and extent of ethanol formation incr ... | 2012 | 24082274 |
| simultaneous saccharification and fermentation of agave tequilana fructans by kluyveromyces marxianus yeasts for bioethanol and tequila production. | agave tequilana fructans (atf) constitute a substrate for bioethanol and tequila industries. as kluyveromyces marxianus produces specific fructanases for atf hydrolysis, as well as ethanol, it can perform simultaneous saccharification and fermentation. in this work, fifteen k. marxianus yeasts were evaluated to develop inoculums with fructanase activity on atf. these inoculums were added to an atf medium for simultaneous saccharification and fermentation. all the yeasts, showed exo-fructanhydrol ... | 2013 | 23941710 |
| impact of high temperature on ethanol fermentation by kluyveromyces marxianus immobilized on banana leaf sheath pieces. | ethanol fermentation was carried out with kluyveromyces marxianus cells at various temperatures (30, 35, 40, and 45 °c). fermentation performance of the immobilized yeast on banana leaf sheath pieces and the free yeast were evaluated and compared. generally, ethanol production of the immobilized and free yeast was stable in a temperature range of 30-40 °c. temperature of 45 °c restricted yeast growth and lengthened the fermentation. the immobilized yeast demonstrated faster sugar assimilation an ... | 2013 | 23900619 |
| ethanol fermentation from xylose by metabolically engineered strains of kluyveromyces marxianus. | we constructed a xylose-fermenting recombinant strain of thermotolerant yeast kluyveromyces marxianus, dmb3-7. both xylose consumption and ethanol production were remarkably increased in dmb3-7 compared to the control strain at 30°c. furthermore, dmb3-7 produced ethanol from xylose at both 42°c and 45°c, above which xylose metabolic activity decreased. | 2013 | 23871200 |
| simultaneous integration of multiple genes into the kluyveromyces marxianus chromosome. | while kluyveromyces marxianus is a promising yeast strain for biotechnological applications, genetic engineering of this strain is still challenging, especially when multiple genes are to be transformed. sequential gene integration, which takes advantage of repetitive insertion/excision of the ura3 gene as a marker, has been the best option until now, because the ura3-deletion mutant is the only precondition for this method. however, we found that the introduced gene is co-excised during the ura ... | 2013 | 23845272 |
| bioethanol production from lignocellulosic biomass by a novel kluyveromyces marxianus strain. | the yeast kluyveromyces marxianus is considered as a potential alternative to saccharomyces cerevisiae in producing ethanol as a biofuel. in this study, we investigated the ethanol fermentation properties of novel k. marxianus strain dmb1, isolated from bagasse hydrolysates. this strain utilized sorbitol as well as various pentoses and hexoses as single carbon sources under aerobic conditions and produced ethanol from glucose in hydrolysates of the japanese cedar at 42 °c. reference strains k. m ... | 2013 | 23832346 |
| yeasts from kefir grains: isolation, identification, and probiotic characterization. | kefir-a traditional beverage whose consumption has been associated with health benefits-is a logical natural product to investigate for new probiotic strains. the aim of the present work was to isolate and identify kefir yeasts and select those with acid and bile tolerance to study their adhesion to epithelial cells and their transit through mouse gut. from 4 milky and 3 sugary kefir grains, 34 yeast strains were isolated and identified by means of classical microbiological and molecular-genetic ... | 2014 | 23824665 |
| [effect of aeration and inulin concentration on ethanol production by kluyveromyces marxinaus yx01]. | consolidated bioprocessing technology can be used for kluyveromyces marxianus yx01 to produce ethanol from jerusalem artichoke, which is one of the potential processes to produce biofuel from non-cereal crops. in this study, we combined the aeration rate with the substrate concentration to conduct cross-over experiments for k. marxinaus yx01, and studied ethanol fermentation and the influence of inulin enzyme activity. the substrate concentration had a little repressive effect on ethanol product ... | 2013 | 23789273 |