| isomaltulose is actively metabolized in plant cells. | isomaltulose is a structural isomer of sucrose (suc). it has been widely used as a nonmetabolized sugar in physiological studies aimed at better understanding the regulatory roles and transport of sugars in plants. it is increasingly used as a nutritional human food, with some beneficial properties including low glycemic index and acariogenicity. cloning of genes for suc isomerases opened the way for direct commercial production in plants. the understanding that plants lack catabolic capabilitie ... | 2011 | 22010106 |
| Recombinant expression, localization and in vitro inhibition of midgut cysteine peptidase (Sl-CathL) from sugarcane weevil, Sphenophorus levis. | A cDNA coding for a digestive cathepsin L, denominated Sl-CathL, was isolated from a cDNA library of Sphenophorus levis larvae, representing the most abundant EST (10.49%) responsible for proteolysis in the midgut. The open reading frame of 972bp encodes a preproenzyme similar to midgut cathepsin L-like enzymes in other coleopterans. Recombinant Sl-CathL was expressed in Pichia pastoris, with molecular mass of about 42kDa. The recombinant protein was catalytically activated at low pH and the mat ... | 2012 | 22100428 |
| isolation of a gastroprotective arabinoxylan from sugarcane bagasse. | after industrial processing, one-third of sugarcane culms is converted into residual bagasse. the xylan-rich hemicellulose components of the bagasse were extracted with hot aqueous alkali (ax-crude). approximately 82% of the extracted hemicelluloses was precipitated with ethanol (ax-pet). both ax-crude and ax-pet contained an arabinoxylan as confirmed by 13c nmr and methylation analysis. fraction ax-pet was fed to female wistar rats with ethanol-induced gastric lesions. oral administrations of 3 ... | 2011 | 21945160 |
| characterization of chloroplast dna microsatellites from saccharum spp and related species. | microsatellites, or simple sequence repeats (ssrs), and their flanking regions in chloroplast genomes (plastomes) of some species of the family poaceae were analyzed in silico to look for dna sequence variations. comparison of the complete chloroplast dna sequences (cpdnas) of sugarcane (saccharum hybrid cv. sp-80-3280 and s. officinarum cv. nco310) and related species, agrostis stolonifera, brachypodium distachyon, hordeum vulgare subsp vulgare, lolium perenne, oryza nivara, o. sativa sub ... | 2011 | 21948764 |
| microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil sphenophorus levis (coleoptera, curculionidae). | the sugarcane weevil, sphenophorus levis, is a wide-spread sugarcane pest in brazil. sphenophorus levis may depend on microorganisms that inhabit its intestinal tract. we examined the diversity of the gut microbiota of s. levis, which was characterized using culture-dependent and culture-independent methods. analysis of 16s rrna amplified directly from the gut community revealed the presence of 14 genera, one group from the candidatus category, one uncultured group assigned to the family f ... | 2011 | 22057964 |
| a comparative study of ph modulation and trace elements of various fruit juices on enamel erosion: an in vitro study. | fruit juices are popular worldwide with children of all ages as they are sweet and perceived to be healthful. this in vitro study was sought to measure ph of 10 different fruit juices, to find out possible erosive effects on human dental enamel of 40 extracted sound premolars and also to measure fluoride and trace elements of these juices. the estimation of ph of fruit juices was done by using systronic uph 362 ph meter. the erosive effects of fruit juices were tested by using polarized light mi ... | 2011 | 21985876 |
| Conceptual design of cost-effective and environmentally-friendly configurations for fuel ethanol production from sugarcane by knowledge-based process synthesis. | In this work, the hierarchical decomposition methodology was used to conceptually design the production of fuel ethanol from sugarcane. The decomposition of the process into six levels of analysis was carried out. Several options of technological configurations were assessed in each level considering economic and environmental criteria. The most promising alternatives were chosen rejecting the ones with a least favorable performance. Aspen Plus was employed for simulation of each one of the tech ... | 2012 | 22137752 |
| holocellulase activity from schizophyllum commune grown on bamboo: a comparison with different substrates. | the natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, environmental technology, and bioconversion. in order to find new lignocellulolytic enzymes and evaluate bamboo fibers as substrate, schizophyllum commune a fungus with broad distribution ... | 2011 | 21964940 |
| quantitative proteomic analysis of lignocellulolytic enzymes by phanerochaete chrysosporium on different lignocellulosic biomass. | lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. the current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. to understand the enzymatic hydrolysis of complex biomasses, in this report, lignocellulolytic enzymes secretion by phanerochaete chrysosporium cultivated in different natural lignocellulosic biomass such as corn stover, hay, sawdust, sugarcane baggas ... | 2011 | 22146477 |
| efficient conversion of sugarcane stalks into ethanol employing low temperature alkali pretreatment method. | an alternative route for bio-ethanol production from sugarcane stalks (juice and bagasse) featuring a previously reported low temperature alkali pretreatment method was evaluated. test-tube scale pretreatment-saccharification experiments were carried out to determine optimal lta pretreatment conditions for sugarcane bagasse with regard to the efficiency of enzymatic hydrolysis of the cellulose. free fermentable sugars and bagasse recovered from 2 kg of sugarcane stalks were jointly converted int ... | 2011 | 22000967 |
| transcriptome analysis of aspergillus niger grown on sugarcane bagasse. | abstract: | 2011 | 22008461 |
| poly(3-hydroxybutyrate) production by bacillus cereus spv using sugarcane molasses as the main carbon source. | the main hindrance in the use of polyhydroxyalkanoates (phas) as a replacement for existing petroleum-based plastics is their high production cost. the carbon source accounts for 50% of the cost for pha production. thus, increasing the yield and productivity of phas on cheap substrates is an important challenge for biotechnologists to support the commercialization and further applications of these polymers. in this study, we have investigated the use of an agricultural raw material, sugarcane mo ... | 2011 | 22147642 |
| Ethanol production by continuous fermentation of d-(+)-cellobiose, d-(+)-xylose and sugarcane bagasse hydrolysate using the thermoanaerobe Caloramator boliviensis. | The recently isolated anaerobic bacterium Caloramator boliviensis with an optimum growth temperature of 60°C can efficiently convert hexoses and pentoses into ethanol. When fermentations of pure sugars and a pentose-rich sugarcane bagasse hydrolysate were carried out in a packed bed reactor with immobilized cells of C. boliviensis, more than 98% of substrates were converted. Ethanol yields of 0.40-0.46g/g of sugar were obtained when sugarcane bagasse hydrolysate was fermented. These features rev ... | 2012 | 22055102 |
| use of sugarcane molasses "b" as an alternative for ethanol production with wild-type yeast saccharomyces cerevisiae itv-01 at high sugar concentrations. | molasses "b" is a rich co-product of the sugarcane process. it is obtained from the second step of crystallization and is richer in fermentable sugars (50-65%) than the final molasses, with a lower non-sugar solid content (18-33%); this co-product also contains good vitamin and mineral levels. the use of molasses "b" for ethanol production could be a good option for the sugarcane industry when cane sugar prices diminish in the market. in a complex medium like molasses, osmotolerance is a desirab ... | 2011 | 21971607 |
| Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil. | Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire ba ... | 2011 | 22042267 |
| Developing a sustainable phytomanagement strategy for excessive selenium in western United States and India. | Phytomanagement technology is recognized as an inexpensive and environmental friendly strategy for managing natural-occurring selenium (Se) in soils and in poor quality waters. Multi-year field and greenhouse studies were conducted with different plant species in California, USA and Punjab, India under high Se growing conditions. Some of the plant species included; canola (Brassica napus), mustard (B. juncea), broccoli (B. oleracea), spearmint (Mentha viridis), sugarcane (Saccharum officcinarum) ... | 2011 | 22046761 |
| Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene codes for 130-kDa toxin protein. | Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene from Bacillus thuringiensis var. kurstaki borne on pKT230, shuttle vector, was generated. PCR amplification of Cry1Ac gene present in recombinant G. diazotrophicus yielded a 278-bp DNA product. The nitrogenase assay has revealed that the recombinant G. diazotrophicus in sugarcane stem produced similar levels of nitrogenase compared to wild-type G. diazotrophicus. The presence of 130-kDa protein in apoplastic fluid from sugarcane ... | 2011 | 21934327 |
| down regulation of a gene for cadherin, but not alkaline phosphatase, associated with cry1ab resistance in the sugarcane borer diatraea saccharalis. | the sugarcane borer, diatraea saccharalis, is a major target pest of transgenic corn expressing bacillus thuringiensis (bt) proteins (i.e., cry1ab) in south america and the mid-southern region of the united states. evolution of insecticide resistance in such target pests is a major threat to the durability of transgenic bt crops. understanding the pests' resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. alterations in expression of c ... | 2011 | 21991350 |
| Enzymatic hydrolysis and simultaneous saccharification and fermentation of alkali/peracetic acid-pretreated sugarcane bagasse for ethanol and 2,3-butanediol production. | The enzymatic digestibility of alkali/peracetic acid (PAA)-pretreated bagasse was systematically investigated. The effects of initial solid consistency, cellulase loading and addition of supplemental ß-glucosidase on the enzymatic conversion of glycan were studied. It was found the alkali-PAA pulp showed excellent enzymatic digestibility. The enzymatic glycan conversion could reach about 80% after 24 h incubation when enzyme loading was 10 FPU/g solid. Simultaneous saccharification and fermentat ... | 2011 | 22112569 |
| molecular characterization of a new strain of sugarcane streak mosaic virus (scsmv). | the complete nucleotide sequences of three sugarcane streak mosaic virus (scsmv) isolates found in sugarcane plants imported from japan (jp1, jp2) and indonesia (id) into china were determined. sequence analysis showed that, based on the whole genome sequence and the nucleotide sequences encoding the ten mature proteins, the three scsmv isolates shared identities of 81.62%-81.67% and 76.39%-85.93%, respectively, with a pakistan (pak) isolate of scsmv. phylogenetic analysis indicated that scsmv-j ... | 2011 | 21927898 |
| functional characterization of sugarcane myb transcription factor gene promoter (pscmybas1) in response to abiotic stresses and hormones. | the sugarcane (saccharum officinarum) stress-related myb transcription factor gene, scmybas1, demonstrated induced response to water deficit and salt stress in our previous study. to elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (pscmybas1, 1,033 bp) flanking the 5' scmybas1 coding region from the sugarcane genome. a series of pscmybas1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -8 ... | 2011 | 22083650 |
| sugarcane hsp101 is a hexameric chaperone that binds nucleotides. | the clp/hsp100 aaa+ chaperone family is involved in recovering aggregated proteins and little is known about other orthologs of the well studied clpb from escherichia coli and hsp104 from saccharomyces cerevisiae. plant hsp101 is a good model for understanding the relationship between the structure and function of hsp100 proteins and to investigate the role of these chaperones in disaggregation processes. here, we present the cloning and purification of a sugarcane ortholog, shsp101, which is ex ... | 2011 | 21903129 |
| acquisition and transmission of peanut clump virus by polymyxa graminis on cereal species. | the objective of this study was to investigate the specificity of the interactions between polymyxa graminis, peanut clump virus (pcv), and cereals, particularly the acquisition and the transmission of the virus by three p. graminis formae speciales. a new strategy has been developed: it involves using sugarcane as the common host for both the virus and its vector in order to produce the viruliferous zoospores of p. graminis f. sp. subtropicalis, temperata, and tropicalis that were then inoculat ... | 2011 | 21916623 |
| the actinobacterium microbacterium sp. 16sh accepts pbbr1-based pprobe vectors, forms biofilms, invades roots, and fixes n(2) associated with micropropagated sugarcane plants. | members of the genus microbacterium lineage of gram-positive actinobacteria are increasingly being reported to display significant traits associated with environmental biotechnology and bioengineering. 16sh is a nitrogen-fixing bacterial strain isolated from a surface-sterilized stem of sugarcane grown in guangxi, china. analysis of 16s rrna gene sequences revealed that 16sh belonged to the genus microbacterium. pprobe-ptet(r) plasmids were constructed by cloning the promoter region of the tet ... | 2011 | 22002067 |
| influence of nutritional and environmental factors on ethanol and endopolygalacturonase co-production by kluyveromyces marxianus ccebi 2011. | ethanol and endopolygalacturonase (endopg) are simultaneously produced by the yeast kluyveromyces marxianus ccebi 2011. the aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. simplex sequential optimization showed that after 15 runs the optimal conditions were: ph, 4.6; temperature, 31 ºc; total redu ... | 2011 | 22015701 |
| sugarcane biopolymer patch in femoral vein angioplasty on dogs. | to evaluate the use of the sugarcane biopolymer membrane in femoral vein patch angioplasty on dogs. | 2011 | 21890306 |
| Cellulase production from agricultural residues by recombinant fusant strain of a fungal endophyte of the marine sponge Latrunculia corticata for production of ethanol. | Several fungal endophytes of the Egyptian marine sponge Latrunculia corticata were isolated, including strains Trichoderma sp. Merv6, Penicillium sp. Merv2 and Aspergillus sp. Merv70. These fungi exhibited high cellulase activity using different lignocellulosic substrates in solid state fermentations (SSF). By applying mutagenesis and intergeneric protoplast fusion, we have obtained a recombinant strain (Tahrir-25) that overproduced cellulases (exo-ß-1,4-glucanase, endo-ß-1,4-glucanase and ß-1,4 ... | 2011 | 21898149 |
| hydrothermal pretreatment of sugarcane bagasse using response surface methodology improves digestibility and ethanol production by ssf. | sugarcane bagasse was characterized as a feedstock for the production of ethanol using hydrothermal pretreatment. reaction temperature and time were varied between 160 and 200°c and 5-20 min, respectively, using a response surface experimental design. the liquid fraction was analyzed for soluble carbohydrates and furan aldehydes. the solid fraction was analyzed for structural carbohydrates and klason lignin. pretreatment conditions were evaluated based on enzymatic extraction of glucose and xylo ... | 2011 | 22080307 |
| Establishment of regeneration and transformation system of sugarcane cultivar GT54-9 (C9). | Plant regeneration protocols for sugarcane GT54-9(C9) cultivar were developed for direct organogenesis and indirect somatic embryogenesis, using young leaf segments as explants by studying the influence of different concentrations and types of cytokinin and auxin hormones. For the callus formation from young leaves, a medium containing 4mg/l 2,4-D was found very effective. For embryo formation, MS medium supplemented with 1mg/l Kin and 0.5 mg/l 2,4-D was used. While in the case of direct organog ... | 2011 | 21912218 |
| Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control. | Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ... | 2011 | 21850475 |
| Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis. | Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180°C for 20 min. Fluorescent-labeled carbohydrate-binding modules which recognize crystalline cellulose (CjCBM3-GFP), non-crystalline cellulose (CjCBM28-GFP) and xylan (CtCBM22-GFP) were app ... | 2011 | 21903385 |
| Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2. | A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen consecutive fermentation cycles, attaining ethanol at 17.1 ± 0.2% (v/v) with a batch productivity of 3.5 ± 0.04 g l(-1) h(-1). Moreover, this innovative operational strategy (biomass ... | 2012 | 21898130 |
| Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone. | Methylotrophic bacteria were isolated from the phyllosphere of different crop plants such as sugarcane, pigeonpea, mustard, potato and radish. The methylotrophic isolates were differentiated based on growth characteristics and colony morphology on methanol supplemented ammonium mineral salts medium. Amplification of the mxaF gene helped in the identification of the methylotrophic isolates as belonging to the genus Methylobacterium. Cell-free culture filtrates of these strains enhanced seed germi ... | 2011 | 22200783 |
| Continuous two stage acetone-butanol-ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313. | The objective of this study was to optimize continuous acetone-butanol-ethanol (ABE) fermentation using a two stage chemostat system integrated with liquid-liquid extraction of solvents produced in the first stage. This minimized end product inhibition by butanol and subsequently enhanced glucose utilization and solvent production in continuous cultures of Clostridium acetobutylicum B 5313. During continuous two-stage ABE fermentation, sugarcane bagasse was used as the cell holding material for ... | 2011 | 22197332 |
| sugarcane genes differentially expressed in response to puccinia melanocephala infection: identification and transcript profiling. | brown rust caused by the fungus puccinia melanocephala is a major disease of sugarcane (saccharum spp.). a sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety b4362, showed a post-haustorial hypersensitive response (hr)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to p. melanocephala via suppression subtractive hybridization (ssh). tester cdna was derived from the brown rust-resistant mutant after inoculation wi ... | 2012 | 22212461 |
| production of xylanase under solid-state fermentation by aspergillus tubingensis jp-1 and its application. | the production of extracellular xylanase by a locally isolated strain of aspergillus tubingensis jp-1 was studied under solid-state fermentation. among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. the influence of various parameters such as initial ph, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. the production of the xylanase reached a peak ... | 2012 | 22271252 |
| fiber fermentability effects on energy and macronutrient digestibility, fecal parameters, postprandial metabolite responses, and colon histology of overweight cats. | considering the different potential benefits of divergent fiber ingredients, the effect of 3 fiber sources on the energy and macronutrient digestibility, fermentation product formation, postprandial metabolite responses, and colon histology of overweight cats fed kibble diets was compared. twenty-four healthy adult cats were assigned in a complete randomized block design to 2 groups of 12 animals, and 3 animals from each group were fed 1 of 4 of the following kibble diets: control (co; 11.5% die ... | 2012 | 22247109 |
| predominant nifh transcript phylotypes related to rhizobium rosettiformans in field-grown sugarcane plants and in norway spruce. | although some sugarcane cultivars may benefit substantially from biological nitrogen fixation (bnf), the responsible bacteria have been not identified yet. here, we examined the active diazotrophic bacterial community in sugarcane roots from africa and america by reverse transcription (rt)-pcr using broad-range nifh-specific primers. denaturing gradient gel electrophoresis (dgge) profiles obtained from sugarcane showed a low diversity at all sample locations with one phylotype amounting up to 10 ... | 2011 | 23761284 |
| in vivo interactions of entomopathogenic fungi, beauveria spp. and metarhizium anisopliae with selected opportunistic soil fungi of sugarcane ecosystem. | in the present study, the interactions of entomopathogenic fungi viz., beauveria bassiana, beauveria brongniartii and metarhizium anisopliae among themselves and three other opportunistic soil fungi from the sugarcane ecosystem namely, fusarium saachari, aspergillus sp. and penecillium sp. were assayed in vivo against galleria mellonella larvae. the tested fungi were co-applied on iv instar g. mellonella @ 1 x 10(7) ml(-1), in combinations of two, at the interval of 24 hrs either preceding or su ... | 2012 | 23359998 |
| chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes. | the plant pathogenic fungus chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84ug(-1)), fpase (2.52ug(-1)), β-glucosidase (21.55ug(-1)) and xylanase (362.38ug(-1)) activities were obtained using wheat bran as carbon source. cellulases and xylanase produced by c. cubensis showed maximal hydrolysis rate at ph 4.0 and in a temperature range of 50-60°c. all enzymatic activities were highly stable at 40 and 50°c through 48h of pre ... | 2012 | 23313674 |
| solvent extraction of antioxidants from steam exploded sugarcane bagasse and enzymatic convertibility of the solid fraction. | solvent extraction of steam exploded lignocellulosic biomass may be a potential way to obtain antioxidative extracts and to enhance the enzymatic convertibility of the solid residue. boiling solvent extraction (bse) showed higher solid and phenolic yields than room temperature extraction. solubilities of phenolics and sugars were higher in anhydrous ethanol (ae) and deionized water (dw) than in ethyl acetate under each individual extraction condition. the antioxidant activities of the ae and dw ... | 2012 | 23280180 |
| rnai-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane. | to engineer trehalose metabolism in sugarcane (saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (tpsp), to increase trehalose biosynthesis and an rnai transgene specific for trehalase, to abrogate trehalose catabolism. in rnai-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. in tpsp lines trehalase activity was significantly higher. no events of co-integratio ... | 2012 | 23539210 |
| [assessment of health-related quality of life of sugarcane cutters in the pre-harvest and harvest periods]. | to assess health-related quality of life in sugarcane cutters. | 2012 | 23503542 |
| description of a unique, complex feeding socket caused by the putative primitive root-knot nematode, meloidogyne kikuyensis. | meloidogyne kikuyensis produces unique galls that form on one side of the root resembling nitrogen-fixing nodules that are produced on legumes in response to infection by rhizobium and related bacteria. the gall caused by this root-knot nematode is made up of a complex feeding socket composed of several giant cells that are ramified with xylem vessels extending perpendicular from the vascular cylinder. the anterior portion of the second-stage juvenile, which develops into an adult, plugs into th ... | 2012 | 23482409 |
| tailor-made fructan synthesis in plants: a review. | fructan, a fructose polymer, is produced by many bacteria and plants. fructan is used as carbohydrate reserve, and in bacteria also as protective outside layer. chicory is a commercial fructan producing crop. the disadvantage of this crop is its fructan breakdown before harvest. studies using genetically modification showed that fructan biosynthesis is difficult to steer in chicory. alternatives for production of tailor-made fructan, fructan with a desired polymer length and linkage type, are or ... | 2012 | 23465900 |
| on the occurrence of p-type plastids in sieve elements of sugarcane short communication. | | 2003 | 187392 |
| [investigation on an outbreak of allergic pulmonary alveolitis]. | 20 allergic alveolitis cases occurred abruptly within 10 days among teachers at a primary school in shanghai. the cases make up 42.6% (20/47) of total staffs of the school. no patient discovered in pupils (137) though they had enough chance to contact with their teachers suffering the disease. roentgenography and clinical findings were criteria of diagnosis. the findings of epidemiologic survey showed that the outbreak was caused by a single exposure of same agent, probably associated with sugar ... | 2009 | 2776192 |
| inhibition of sucrose phosphatase by sucrose. | 1. partially purified sucrose phosphatase from immature stem tissue of sugarcane is inhibited by sucrose. the enzyme was also inhibited by maltose, melezitose and 6-kestose but not by eight other sugars, including glucose and fructose. 2. the relative effectiveness of sucrose, maltose and melezitose as inhibitors is different for sucrose phosphatase from different plants. 3. the inhibition of the sugar-cane enzyme by sucrose was shown to be partially competitive. the k(i) for sucrose is about 10 ... | 2014 | 4291490 |
| metaproteomic analysis of ratoon sugarcane rhizospheric soil. | the current study was undertaken to elucidate the mechanism of yield decline in ratoon sugarcane using soil metaproteomics combined with community level physiological profiles (clpp) analysis. | 2013 | 23773576 |
| molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at são paulo state. | fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. ability to fix nitrogen was verified by the acetylene reduction assay. all potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifh probe derived from azospirillum brasilense. the strains were characterized by rapd, ardra and 16s rdna sequence analysis. rapd analyse ... | 2008 | 24031239 |
| quantification of natural populations of gluconacetobacter diazotrophicus and herbaspirillum spp. in sugar cane (saccharum spp.) using differente polyclonal antibodies. | the species gluconacetobacter diazotrophicus, herbaspirillum seropedicae and h. rubrisubalbicans are endophytic n2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. however, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in n free semi-solid media which are only semi-selective for the species/genera [the most probable number (mpn) techniqu ... | 2009 | 24031435 |
| drought tolerance conferred to sugarcane by association with gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways. | sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. here we aim to study the effects of the association between the diazotroph gluconacetobacter diazotrophicus pal5 and sugarcane cv. sp70-1143 during water depletion by characterizing differential transcriptome pr ... | 2014 | 25489849 |
| the determination of assay for laccase of bacillus subtilis wpi with two classes of chemical compounds as substrates. | ligninolytic enzyme complexes are involved in lignin degradation. among them laccases are outstanding because they use molecular oxygen as a co-substrate instead of hydrogen peroxide as used by peroxidases. bacterial laccase of bacillus genus was first reported in claus and filip (microbiol res 152:209-216, 1997), since then more bacterial laccases have been found. in this research, laccase-producing bacteria were screened from pulp and paper industry wastewater, bagass and sugarcane rhizosphere ... | 2012 | 24293734 |
| structure and function of a novel cellulase 5 from sugarcane soil metagenome. | cellulases play a key role in enzymatic routes for degradation of plant cell-wall polysaccharides into simple and economically-relevant sugars. however, their low performance on complex substrates and reduced stability under industrial conditions remain the main obstacle for the large-scale production of cellulose-derived products and biofuels. thus, in this study a novel cellulase with unusual catalytic properties from sugarcane soil metagenome (cele1) was isolated and characterized. the polype ... | 2013 | 24358302 |
| isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail achatina fulica. | the enzymatic hydrolysis of cellulose by cellulases is one of the major limiting steps in the conversion of lignocellulosic biomass to yield bioethanol. to overcome this hindrance, significant efforts are underway to identify novel cellulases. the snail achatina fulica is a gastropod with high cellulolytic activity, mainly due to the abundance of glycoside hydrolases produced by both the animal and its resident microbiota. in this study, we partially assessed the cellulolytic aerobic bacterial d ... | 2015 | 26347735 |
| assessment of gut bacteria for a paratransgenic approach to control dermolepida albohirtum larvae. | bacteria from the hindguts of dermolepida albohirtum larvae were assessed for their potential to be used in paratransgenic strategies that target scarab pests of sugarcane. bacteria isolated in pure culture from the hindguts of d. albohirtum larvae were from the proteobacteria, firmicutes, and actinobacteria phyla and matched closely with taxa from intestinal and rhizosphere environments. however, these isolates were not the most common gut-associated bacteria identified in denaturing gradient g ... | 2008 | 18456847 |
| genomic insights into strategies used by xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels. | xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. x. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of xanthomonas. for example, this species produces a potent dna gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. a first manuscript on the complete genome sequence of the highly pathogenic x. albilin ... | 2012 | 23171051 |
| insecticide-degrading burkholderia symbionts of the stinkbug naturally occupy various environments of sugarcane fields in a southeast island of japan. | the stinkbug cavelerius saccharivorus, which harbors burkholderia species capable of degrading the organophosphorus insecticide, fenitrothion, has been identified on a japanese island in farmers' sugarcane fields that have been exposed to fenitrothion. a clearer understanding of the ecology of the symbiotic fenitrothion degraders of burkholderia species in a free-living environment is vital for advancing our knowledge on the establishment of degrader-stinkbug symbiosis. in the present study, we ... | 2014 | 25736865 |
| a fine-scale phylogenetic analysis of free-living burkholderia species in sugarcane field soil. | the diversity and abundance of burkholderia species in sugarcane field soils were investigated by a 16s rrna gene-based approach using genus-specific primers. a total of 365,721 sequences generated by the illumina miseq platform were assigned to the genus burkholderia. nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. quantitative pcr analysis revealed a consistent number of 16s rrna gene copies for burkholderia species (10(7) g(-1) soil) ac ... | 2014 | 25410730 |
| evidence of environmental and vertical transmission of burkholderia symbionts in the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae). | the vertical transmission of symbiotic microorganisms is omnipresent in insects, while the evolutionary process remains totally unclear. the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae), is a serious sugarcane pest, in which symbiotic bacteria densely populate the lumen of the numerous tubule-like midgut crypts that the chinch bug develops. cloning and sequence analyses of the 16s rrna genes revealed that the crypts were dominated by a specific group of bacteria belongi ... | 2014 | 25038101 |
| isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of china and optimization of cellulase production by paenibacillus terrae me27-1. | from different natural reserves in the subtropical region of china, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after congo-red staining. molecular identification showed that the 22 strains belonged to 10 different genera, with the burkholderia genus exhibiting the highest strain diversity and accounting for 36.36 ... | 2014 | 25050355 |
| prospection and evaluation of (hemi) cellulolytic enzymes using untreated and pretreated biomasses in two argentinean native termites. | saccharum officinarum bagasse (common name: sugarcane bagasse) and pennisetum purpureum (also known as napier grass) are among the most promising feedstocks for bioethanol production in argentina and brazil. in this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with nasutitermes aquilinus and cortaritermes fulviceps termite gut digestome. the chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellul ... | 2015 | 26313257 |
| crosstalk between sugarcane and a plant-growth promoting burkholderia species. | bacterial species in the plant-beneficial-environmental clade of burkholderia represent a substantial component of rhizosphere microbes in many plant species. to better understand the molecular mechanisms of the interaction, we combined functional studies with high-resolution dual transcriptome analysis of sugarcane and root-associated diazotrophic burkholderia strain q208. we show that burkholderia q208 forms a biofilm at the root surface and suppresses the virulence factors that typically trig ... | 2016 | 27869215 |
| bacterial abilities and adaptation toward the rhizosphere colonization. | the rhizosphere harbors one of the most complex, diverse, and active plant-associated microbial communities. this community can be recruited by the plant host to either supply it with nutrients or to help in the survival under stressful conditions. although selection for the rhizosphere community is evident, the specific bacterial traits that make them able to colonize this environment are still poorly understood. thus, here we used a combination of community level physiological profile (clpp) a ... | 2016 | 27610108 |
| global analysis of mannitol 2-dehydrogenase in lactobacillus reuteri crl 1101 during mannitol production through enzymatic, genetic and proteomic approaches. | several plants, fungi, algae, and certain bacteria produce mannitol, a polyol derived from fructose. mannitol has multiple industrial applications in the food, pharmaceutical, and medical industries, being mainly used as a non-metabolizable sweetener in foods. many heterofermentative lactic acid bacteria synthesize mannitol when an alternative electron acceptor such as fructose is present in the medium. in previous work, we reported the ability of lactobacillus reuteri crl 1101 to efficiently pr ... | 2017 | 28060932 |
| draft genome sequence of pseudomonas sp. strain lfm046, a producer of medium-chain-length polyhydroxyalkanoate. | pseudomonas sp. lfm046 is a medium-chain-length polyhydroxyalkanoate (phamcl) producer capable of using various carbon sources (carbohydrates, organic acids, and vegetable oils) and was first isolated from sugarcane cultivation soil in brazil. the genome sequence was found to be 5.97 mb long with a g+c content of 66%. | 2015 | 26294616 |
| evaluation of monocot and eudicot divergence using the sugarcane transcriptome. | over 40,000 sugarcane (saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and dna sequences from other angiosperms, including the genomes of arabidopsis and rice (oryza sativa). approximately two-thirds of the sugarcane transcriptome have similar sequences in arabidopsis. these sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angi ... | 2004 | 15020759 |
| the type iii secretion system is necessary for the development of a pathogenic and endophytic interaction between herbaspirillum rubrisubalbicans and poaceae. | herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. h. rubrisubalbicans can also associate with various plants of economic interest in a non pathogenic manner. | 2012 | 22672506 |
| isolation and characterization of scglud2, a new sugarcane beta-1,3-glucanase d family gene induced by sporisorium scitamineum, aba, h2o2, nacl, and cdcl2 stresses. | beta-1,3-glucanases (ec 3.2.1.39), commonly known as pathogenesis-related (pr) proteins, play an important role not only in plant defense against fungal pathogens but also in plant physiological and developmental processes. however, only a limited number of sugarcane beta-1,3-glucanase genes have been isolated. in the present study, we identified and characterized a new beta-1,3-glucanase gene scglud2 (genbank acc no. kf664181) from sugarcane. an x8 domain was present at the c terminal region of ... | 2016 | 27642288 |
| photoautotrophic production of d-lactic acid in an engineered cyanobacterium. | the world faces the challenge to develop sustainable technologies to replace thousands of products that have been generated from fossil fuels. microbial cell factories serve as promising alternatives for the production of diverse commodity chemicals and biofuels from renewable resources. for example, polylactic acid (pla) with its biodegradable properties is a sustainable, environmentally friendly alternative to polyethylene. at present, pla microbial production is mainly dependent on food crops ... | 2013 | 24274114 |
| genomic analysis of xanthomonas translucens pathogenic on wheat and barley reveals cross-kingdom gene transfer events and diverse protein delivery systems. | in comparison to dicot-infecting bacteria, only limited numbers of genome sequences are available for monocot-infecting and in particular cereal-infecting bacteria. herein we report the characterisation and genome sequence of xanthomonas translucens isolate dar61454 pathogenic on wheat and barley. based on phylogenetic analysis of the atp synthase beta subunit (atpd) gene, dar61454 is most closely related to other x. translucens strains and the sugarcane- and banana- infecting xanthomonas strain ... | 2014 | 24416331 |
| sugarcane transcriptome analysis in response to infection caused by acidovorax avenae subsp. avenae. | sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. crop productivity is negatively affected by acidovorax avenae subsp avenae (aaa), which causes the red stripe disease. little is known about the molecular mechanisms triggered in response to the infection. we have investigated the molecular mechanism activated in sugarcane using a rna-seq approach. we have produced a de novo transcriptome assembly (tr7) from sugarcane rna-seq libraries submitted to drought an ... | 2016 | 27936012 |
| comparative proteomics reveals that central metabolism changes are associated with resistance against sporisorium scitamineum in sugarcane. | sugarcane smut, which is caused by sporisorium scitamineum, has been threatening global sugarcane production. breeding smut resistant sugarcane varieties has been proven to be the most effective method of controlling this particular disease. however, a lack of genome information of sugarcane has hindered the development of genome-assisted resistance breeding programs. furthermore, the molecular basis of sugarcane response to s. scitamineum infection at the proteome level was incomplete and combi ... | 2016 | 27733120 |
| de novo analysis of transcriptome reveals genes associated with leaf abscission in sugarcane (saccharum officinarum l.). | sugarcane (saccharum officinarum l.) is an important sugar crop which belongs to the grass family and can be used for fuel ethanol production. the growing demands for sugar and biofuel is asking for breeding a sugarcane variety that can shed their leaves during the maturity time due to the increasing cost on sugarcane harvest. | 2016 | 26946183 |
| draft genome sequences of xanthomonas sacchari and two banana-associated xanthomonads reveal insights into the xanthomonas group 1 clade. | we present draft genome sequences for three strains of xanthomonas species, each of which was associated with banana plants (musa species) but is not closely related to the previously sequenced banana-pathogen xanthomonas campestris pathovar musacearum. strain ncppb4393 had been deposited as xanthomonas campestris pathovar musacearum but in fact falls within the species xanthomonas sacchari. strain ncppb1132 is more distantly related to xanthomonas sacchari whilst strain ncppb 1131 grouped in a ... | 2011 | 24710305 |
| genome sequencing of xanthomonas vasicola pathovar vasculorum reveals variation in plasmids and genes encoding lipopolysaccharide synthesis, type-iv pilus and type-iii secretion effectors. | xanthomonas vasicola pathovar vasculorum (xvv) is the bacterial agent causing gumming disease in sugarcane. here, we compare complete genome sequences for five isolates of xvv originating from sugarcane and one from maize. this identified two distinct types of lipopolysaccharide synthesis gene clusters among xvv isolates: one is similar to that of xanthomonas axonopodis pathovar citri (xac) and is probably the ancestral type, while the other is similar to those of the sugarcane-inhabiting specie ... | 2014 | 25437615 |
| full genome sequence analysis of two isolates reveals a novel xanthomonas species close to the sugarcane pathogen xanthomonas albilineans. | xanthomonas albilineans is the bacterium responsible for leaf scald, a lethal disease of sugarcane. within the xanthomonas genus, x. albilineans exhibits distinctive genomic characteristics including the presence of significant genome erosion, a non-ribosomal peptide synthesis (nrps) locus involved in albicidin biosynthesis, and a type 3 secretion system (t3ss) of the salmonella pathogenicity island-1 (spi-1) family. we sequenced two x. albilineans-like strains isolated from unusual environments ... | 2015 | 26213974 |
| multi-analytical approach reveals potential microbial indicators in soil for sugarcane model systems. | this study focused on the effects of organic and inorganic amendments and straw retention on the microbial biomass (mb) and taxonomic groups of bacteria in sugarcane-cultivated soils in a greenhouse mesocosm experiment monitored for gas emissions and chemical factors. the experiment consisted of combinations of synthetic nitrogen (n), vinasse (v; a liquid waste from ethanol production), and sugarcane-straw blankets. increases in co2-c and n2o-n emissions were identified shortly after the additio ... | 2015 | 26057123 |
| what makes xanthomonas albilineans unique amongst xanthomonads? | xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. compared to other species of xanthomonas, x. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy. its genome, which has experienced significant erosion, has unique genomic features. it lacks two loci required for pathogenicity in other plant pathogenic species of xanthomonas: the xanthan gum biosynthesis and the hrp-t3ss (hypersensitive response and pathogenicity-type three secretion system) gene c ... | 2015 | 25964795 |
| rnaseq transcriptional profiling following whip development in sugarcane smut disease. | sugarcane smut disease is caused by the biotrophic fungus sporisorium scitamineum. the disease is characterized by the development of a whip-like structure from the primary meristems, where billions of teliospores are produced. sugarcane smut also causes tillering and low sucrose and high fiber contents, reducing cane productivity. we investigated the biological events contributing to disease symptoms in a smut intermediate-resistant sugarcane genotype by examining the transcriptional profiles ( ... | 2016 | 27583836 |
| plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in guangxi, china. | the current nitrogen fertilization for sugarcane production in guangxi, the major sugarcane-producing area in china, is very high. we aim to reduce nitrogen fertilization and improve sugarcane production in guangxi with the help of indigenous sugarcane-associated nitrogen-fixing bacteria. we initially obtained 196 fast-growing bacterial isolates associated with the main sugarcane cultivar roc22 plants in fields using a nitrogen-deficient minimal medium and screened out 43 nitrogen-fixing isolate ... | 2012 | 22510648 |
| reproducible rna preparation from sugarcane and citrus for functional genomic applications. | high-throughput functional genomic procedures depend on the quality of the rna used. copurifying molecules can negatively impact the functionality of some plant rna preparations employed in these procedures. we present a simplified, rapid, and scalable sds/phenol-based method that provides the high-quantity and -quality rna required by the newly emerging biotechnology applications. the method is applied to isolating rna from tissues of two biotechnologically important crop plants, sugarcane and ... | 2009 | 20148085 |
| symbiotic potential and survival of native rhizobia kept on different carriers. | native rhizobia are ideal for use as commercial legume inoculants. the characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. the objective of this study was to investigate the effects of peat (peat), perlite sugarcane bagasse (psb), carboxymethyl cellulose plus starch (cmcs), and yeast extract mannitol supplemented with mannitol (yemm) on the survival, nodulation potential and n2 fixation capacity of the native strains ... | 2015 | 26413054 |
| optimization of pretreatment and fermentation conditions for production of extracellular cellulase complex using sugarcane bagasse. | sugarcane bagasse (scb), a lignocellulosic byproduct of juice extraction from sugarcane, is rich in cellulose (40-42%). this could be used as a substrate for the production of cellulase complex. fermentation conditions were optimized for production of cellulase complex (cmcase, cellulobiase and fpase) by wild type trichoderma sp. using sugarcane bagasse as sole carbon source. alkaline treatment (2% naoh) of bagasse (alscb) was found suitable for the production of reducing sugar over the acidic p ... | 2014 | 25489168 |
| essential genes for in vitro growth of the endophyte herbaspirillum seropedicae smr1 as revealed by transposon insertion site sequencing. | the interior of plants contains microorganisms (referred to as endophytes) that are distinct from those present at the root surface or in the surrounding soil. herbaspirillum seropedicae strain smr1, belonging to the betaproteobacteria, is an endophyte that colonizes crops, including rice, maize, sugarcane, and sorghum. different approaches have revealed genes and pathways regulated during the interactions of h. seropedicae with its plant hosts. however, functional genomic analysis of transposon ... | 2016 | 27590816 |
| specific plant induced biofilm formation in methylobacterium species. | two endophytic strains of methylobacterium spp. were used to evaluate biofilm formation on sugarcane roots and on inert wooden sticks. results show that biofilm formation is variable and that plant surface and possibly root exudates have a role in methylobacterium spp. host recognition, biofilm formation and successful colonization as endophytes. | 2011 | 24031703 |
| production of tea vinegar by batch and semicontinuous fermentation. | the fermented tea vinegar combines the beneficial properties of tea and vinegar. the complete fermentation takes 4 to 5 weeks in a batch culture and thus can be shortened by semi continuous/ continuous fermentation using immobilized bacterial cells. in the present study, alcoholic fermentation of 1.0 and 1.5% tea infusions using saccharomyces cerevisae g was carried out that resulted in 84.3 and 84.8% fermentation efficiency (fe) respectively. the batch vinegar fermentation of these wines with a ... | 2010 | 23572817 |
| ethanol production in brazil: a bridge between science and industry. | in the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in brazil. these contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. better control of fermentation conditions can ... | 2016 | 27818090 |
| influence of vinasse application in the structure and composition of the bacterial community of the soil under sugarcane cultivation. | although the use of vinasse as a waste helps replenish soil nutrients and improves the quality of the sugarcane crop, it is known that vinasse residues alter the diversity of bacteria naturally present in the soil. the actual impacts of vinasse application on the selection of bacterial taxa are not understood because no studies have addressed this phenomenon directly. analysis of 16s rrna gene clone sequences from four soil types showed that the soil planted with sugarcane and fertilized with vi ... | 2016 | 27528875 |
| isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane. | endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. u-thong 3; ut3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (n)-free sand. eighty nine isolates of endophytic bacteria were obtained on n-free agar. an acetylene reduction assay (ara) detected nitrogenase activity in all 89 isolates. three isolates from the chewing (c2hl2, c7hl1 and c34mr1) sugarcane and one isolate from the industrial sugarcane (ut3r1) varieties wer ... | 2015 | 26868592 |
| identification of genes involved in indole-3-acetic acid biosynthesis by gluconacetobacter diazotrophicus pal5 strain using transposon mutagenesis. | gluconacetobacter diazotrophicus is a beneficial nitrogen-fixing endophyte found in association with sugarcane plants and other important crops. beneficial effects of g. diazotrophicus on sugarcane growth and productivity have been attributed to biological nitrogen fixation process and production of phytohormones especially indole-3-acetic acid (iaa); however, information about the biosynthesis and function of iaa in g. diazotrophicus is still scarce. therefore, the aim of this work was to ident ... | 2016 | 27774087 |
| ethanol fermentation of sugarcane molasses by zymomonas mobilis mtcc 92 immobilized in luffa cylindrica l. sponge discs and ca-alginate matrices. | bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, zymomonas mobilis mtcc 92 entrapped in luffa (luffa cylindrica l.) sponge discs and ca-alginate gel beads as the immobilizing matrices. at the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and ca-alginate entrapped z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. there was no statisti ... | 2012 | 24031981 |
| effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production. | this study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. the chemical composition of these feedstocks was determined. conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. in single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the con ... | 2012 | 24031984 |
| production of bioethanol using agricultural waste: banana pseudo stem. | india is amongst the largest banana (musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. two fungal strains aspergillus ellipticus and aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse wer ... | 2014 | 25477922 |
| optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture. | hydrogen is regarded as an attractive future energy carrier for its high energy content and zero co2 emission. currently, the majority of hydrogen is generated from fossil fuels. however, from an environmental perspective, sustainable hydrogen production from low-cost lignocellulosic biomass should be considered. thermophilic hydrogen production is attractive, since it can potentially convert a variety of biomass-based substrates into hydrogen at high yields. | 2014 | 25184001 |
| bioethanol production from fermentable sugar juice. | bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. this paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarca ... | 2014 | 24715820 |
| finished genome of zymomonas mobilis subsp. mobilis strain cp4, an applied ethanol producer. | zymomonas mobilis subsp. mobilis is one of the most rigorous ethanol-producing organisms known to date, considered by many to be the prokaryotic alternative to yeast. the two most applied z. mobilis subsp. mobilis strains, zm4 and cp4, derive from recife, brazil, and have been isolated from sugarcane fermentations. of these, zm4 was the first z. mobilis representative strain to be sequenced and analyzed. here, we report the finishing of the genome sequence of strain cp4, which is highly similar ... | 2014 | 24407627 |
| response surface optimization of bioethanol production from sugarcane molasses by pichia veronae strain hsc-22. | pichia veronae strain hsc-22 (accession number kp012558) showed a good tolerance to relatively high temperature, ethanol and sugar concentrations. response surface optimization based on central composite design of experiments predicted the optimal values of the influencing parameters that affect the production of bioethanol from sugarcane molasses to be as follows: initial ph 5, 25% (w : v) initial molasses concentration, 35°c, 116 rpm, and 60 h. under these optimum operating conditions the maxi ... | 2015 | 26779347 |
| emerging technologies for the production of renewable liquid transport fuels from biomass sources enriched in plant cell walls. | plant cell walls are composed predominantly of cellulose, a range of non-cellulosic polysaccharides and lignin. the walls account for a large proportion not only of crop residues such as wheat straw and sugarcane bagasse, but also of residues of the timber industry and specialist grasses and other plants being grown specifically for biofuel production. the polysaccharide components of plant cell walls have long been recognized as an extraordinarily large source of fermentable sugars that might b ... | 2016 | 28018390 |
| characterization of three plant biomass-degrading microbial consortia by metagenomics- and metasecretomics-based approaches. | the selection of microbes by enrichment on plant biomass has been proposed as an efficient way to develop new strategies for lignocellulose saccharification. here, we report an in-depth analysis of soil-derived microbial consortia that were trained to degrade once-used wheat straw (ws1-m), switchgrass (sg-m) and corn stover (cs-m) under aerobic and mesophilic conditions. molecular fingerprintings, bacterial 16s ribosomal rna (rrna) gene amplicon sequencing and metagenomic analyses showed that th ... | 2016 | 27418359 |