| modification of the t, k and nk cell population in experimental infection of mice with aspergillus niger. | a study has been made of the cellular response by t, k and nk cells in mice experimentally infected with aspergillus niger. the tests were made on mice treated with 5 x 10(5) conidia. the lymphocyte populations in the blood, spleen and pulmonary lymphnodes have studied to identify possible modifications in immune response in time. the results obtained show that in experimentally infected mice with respect to uninfected mice there is an increase in t-lymphocytes, particularly in the spleen, a per ... | 1986 | 3490617 |
| high hbsag and anti-delta carrier rate among asymptomatic africans living on the campus of the university of niamey, niger. | in 1984 and 1985, a non-random survey was conducted among healthy africans living on the campus of the university of niamey, niger. of 238 asymptomatic subjects, 17.6% carried hepatitis b surface antigen (hbsag) in their serum, while 50.8% demonstrated protective anti-hbs antibodies. antibodies to delta virus were detected in 29.3% of hbsag-positive sera. this suggests that hepatitis b virus (hbv) and hepatitis delta virus (hdv) are common in niger, and the factors that increase the likelihood o ... | 1987 | 3503422 |
| anthropometric estimation of basal metabolic rate in nigeria children and adolescents of niger delta region of nigeria. | most data on basal metabolic rates (bmr) obtained are from developed countries of the world and a few from asian countries. no literature is available on bmr in nigerian children, hence this investigation was carried out in a total of 801 randomly sampled human subjects 606 of whom are children (ages 6 to 17 years) and 195 adolescents' (ages 14 to 19 years). among the children, 264 were boys while 342 were girls. in adolescents, 119 were boys and 76 were girls. it was found that the bmr in niger ... | 1986 | 3508635 |
| an assessment of the fungicidal activity of antimicrobial agents used for hard-surface and skin disinfection. | trichophyton mentagrophytes and aspergillus niger conidia and candida albicans blastospores were employed to assess the fungicidal activity of a range of antimicrobial agents used for hard-surface and skin disinfection in hospitals in the u.k. the antimicrobials were tested at in-use concentrations. the time taken to give greater than 99.99% kill was determined by estimating the number of survivors (cfu/ml) at a variety of time intervals after exposure of washed suspensions of spores to the disi ... | 1986 | 3528229 |
| studies on the evaluation of preservative efficacy--ii. the determination of antimicrobial characteristics of benzylalcohol. | a successful kinetic approach to the screening of preservation efficacy of benzylalcohol--a neutral-type preservative--is described. the d-value, activation energy (ea), temperature coefficient (q10) and concentration exponent (n) were used as parameters in determining the influence of different factors on the efficacy of benzylalcohol. factors, such as the ph of the solution, temperature and concentration of the preservative were investigated. the study was carried out using five micro-organism ... | 1986 | 3531242 |
| inhibition of complement by culture supernatants of aspergillus fumigatus. | aspergillus fumigatus produced a water-soluble extracellular material that inhibited the opsonization of fungal cells by normal human serum. blastospores of cryptococcus neoformans and conidia from a. fumigatus or aspergillus niger were used as fungal targets for ingestion by human monocytes. the opsonic inhibitor from a. fumigatus was found to decrease binding of complement component c3b to fungal surfaces by selectively interfering with activation of the alternative complement pathway. inhibit ... | 1986 | 3537152 |
| direct fermentation of potato starch to ethanol by cocultures of aspergillus niger and saccharomyces cerevisiae. | direct fermentation of unhydrolyzed potato starch to ethanol by monocultures of an amylolytic fungus, aspergillus niger, and cocultures of a. niger and saccharomyces cerevisiae was investigated. amylolytic activity, rate and amount of starch utilization, and ethanol yields increased several-fold in coculture versus the monoculture due to the synergistic metabolic interactions between the species. optimal ethanol yields were obtained in the ph range 5 to 6 and amylolytic activity was obtained in ... | 1986 | 3539016 |
| bacteria and helminth isolates from bile and faeces of zebu cattle slaughtered for human consumption in the niger delta areas of nigeria. | random bacteriological examination of 300 bile samples aseptically drawn from the gallbladders of adult cattle at slaughter revealed the presence of escherichia coli in 30%, enterobacter aerogenes in 14% and klebsiella aerogenes in 56%. bacteriological examination of another 116 faecal samples revealed esch. coli in 60% and ent. aerogenes in 40%, while parasitological examination revealed 50-100 strongyle ova per gram from pre-rain samples of faeces, 100-850 strongyle eggs per gram for faeces co ... | 1986 | 3539044 |
| the status of human filariasis in relation to clinical signs in endemic areas of the niger delta. | a study was carried out in selected parts of the niger delta basin on human filariasis and its relationship to clinical signs. one hundred and seventy-six (13.0%) of 1351 individuals examined were infected with microfilaria. forty-six percent of microfilaria positive cases had m. perstans; 25.6% had w. bancrofti, 19.3% had l. loa, and 9.0% had o. volvulus. the prevalence of filariasis increased with age. microfilarial density was generally low, 28.4% of infected individuals had counts of 50-100 ... | 1986 | 3539045 |
| protease production with sweet potato residue by solid state fermentation. | the sweet potato residue being at the initial moisture content 50-58%, initial ph 3.5-4.3, supplemented with rice bran, and minerals, and incubated at 20-26 degrees c for 5 days was the optimal conditions for protease production with aspergillus niger ntu-am-1 by solid state fermentation. protease could be recovered by shaking at room temperature for one hour and extracted with five times volume of 0.1% nacl solution. the yield of protease was 814 units per gram dry weight of substrate. partiall ... | 1986 | 3545704 |
| synthesis and biocidal activity of zn(ii) complexes of some 2,2'-substituted diphenylamines. | binary as well as ternary complexes of zn(ii) with diphenylamine-2,2'-dicarboxylic acid (dpdc), diphenylamine-2-amino-2'-carboxylic acid (dpac), diphenylamine-2-hydroxy-2'-carboxylic acid (dphc), diphenylamine-2-mercapto-2'-carboxylic acid (dpmc), and n-(2-pyridino) anthranilic acid (npa) have been synthesized and characterized by their elemental analysis, ir spectral data, and molar conductance measurements. antimicrobial activity of these ligands and their respective zn(ii) complexes have been ... | 1987 | 3553430 |
| antifungal activity of ajoene derived from garlic. | the antifungal activity of six fractions derived from garlic was investigated in an in vitro system. ajoene had the strongest activity in these fractions. the growth of both aspergillus niger and candida albicans was inhibited by ajoene at less than 20 micrograms/ml. | 1987 | 3555334 |
| anaplasmosis of small ruminants in nigeria: incidence and parasite identification through blood smear and latex agglutination test (lat). | a survey was carried out on the incidence of anaplasmosis in small ruminants at bodija abattoir, ibadan. 100 sheep and 200 goats were examined using and comparing two methods-the giemsa stained blood smear and serodiagnosis by latex agglutination technique. the sheep and goats examined were among those brought from various parts of northern states and some neighbouring countries like chad, benin, niger and burkina faso. 27% of the sheep and 17.5% of goats were positive for anaplasma bodies with ... | 1986 | 3557833 |
| role of selective pressure by screening organisms in the development of producer mutant isolates of different antibiotic markers. | the phenotypes of producer mutant isolates when obtained after mutagenesis of the spontaneous degenerate aspergillus versicolor n5 by screening on trichophyton rubrum and those on a. niger g3br were found to be markedly different. in respect of phenotypic character anti-t. rubrum producer mutant isolates resembled the parental type more closely (which is specifically active against t. rubrum and generally against dermatophytes) than anti-a. niger mutant isolates. this clearly indicates not only ... | 1986 | 3558163 |
| structural studies of sialylated oligosaccharides of human midcycle cervical mucin. | it was previously shown that reductive alkali treatment of purified human cervical mucin releases a heterogeneous population of reduced neutral, sialylated, and sulfated oligosaccharides (yurewicz, e. c., and moghissi, k. s. (1981) j. biol. chem. 256, 11895-11904). four major sialylated oligosaccharide fractions were isolated with approximate compositions of fuc:glcnac:gal:neuac:n-acetylgalactosaminitol (galnacol) = 0:0:0:1:1 (b1a), 0:0:1:1:1 (b2b), 0:1:2:1:1 (b3a), and 1:1:2:1:1 (b4a), where fu ... | 1987 | 3558366 |
| presence and regulation of atp:citrate lyase from the citric acid producing fungus aspergillus niger. | atp:citrate lyase (ec 4.1.3.8) has been identified in cell-free extracts from the filamentous fungus aspergillus niger. the enzyme was located in the cytosol. it exhibits an activity at least ten times that of acetate-coa-kinase (ec 6.2.1.1) during growth on carbohydrates as carbon sources, and is thus considered responsible for acetyl-coa formation under these conditions. it is formed constitutively and its biosynthesis does not appear to be controlled by changes in the nitrogen or carbon sourc ... | 1987 | 3579463 |
| mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. 1--sugar fungi and natural occurrence of mycotoxins. | sixty-four species and 2 varieties which belong to nineteen genera of fungi were identified from 40 peanut seed samples collected from different places in egypt by using a dilution-plate method on glucose-czapek's medium. the most frequent genera were aspergillus (21 species & 2 varieties), penicillium (16 species) and fusarium (6 species). a. flavus, a. fumigatus, a. niger, p. chrysogenum and f. oxysporum were the most common fungal species. forty seven percent of the samples proved to be toxic ... | 1987 | 3587340 |
| preparation, characterization, and potential application of an immobilized glucose oxidase. | a simple, one-step process, using 0.25 m p-benzoquinone dissolved in 20% dioxane at 50 degrees c for 24 h was applied to the activation of polyacrylamide beads. the activated beads were reacted with glucose oxidase isolated from aspergillus niger. the coupling reaction was performed in 0.1 m potassium phosphate at ph 8.5 and 0-4 degrees c for 24 h. the protein concentration was 50 mg/ml. in such conditions, the highest activity achieved was about 100 u/g solid. the optimum ph for the catalytic a ... | 1987 | 3592653 |
| enzyme-catalysed non-oxidative decarboxylation of aromatic acids: i. purification and spectroscopic properties of 2,3 dihydroxybenzoic acid decarboxylase from aspergillus niger. | in order to understand the molecular mechanism of non-oxidative decarboxylation of aromatic acids observed in microbial systems, 2,3 dihydroxybenzoic acid (dhba) decarboxylase from aspergillus niger was purified to homogeneity by affinity chromatography. the enzyme (mr 120 kda) had four identical subunits (28 kda each) and was specific for dhba. it had a ph optimum of 5.2 and km was 0.34 mm. the decarboxylation did not require any cofactors, nor did the enzyme had any pyruvoyl group at the activ ... | 1987 | 3593354 |
| the activation of aspergillus niger catalase by sodium n-dodecyl-sulphate. | in marked contrast to most enzymes it is found that at ph 6.4 the activity of the fungal catalase from aspergillus niger is increased on binding of sodium n-dodecyl sulphate (sds). activation of the enzyme by up to 180% is found under optimum conditions when approx. 150 sds molecules are bound. activation does not occur under acid (ph 3.2) or alkaline (ph 10.0) conditions. sedimentation analysis confirms that the enzyme does not dissociate into subunits at ph 6.4 (or ph 10.0). these observations ... | 1987 | 3593744 |
| [immunomodulation with natural products. i. effect of an aqueous extract of raphanus sativus niger on experimental influenza infection in mice]. | a raphanus sativus niger water extract was administered by intranasal instillations to mice before inoculation of the influenza virus a/pr 8/34 (h1n1) strain by the same route. the extract ensured some protection against the experimental influenza infection. a significant decrease of the hemagglutinin titre of the mouse lung homogenate was noted, as well as a decrease of the mortality rate and a significant increase of the rate of survival as compared to the untreated controls. | 1987 | 3617495 |
| physico-chemical interaction of mycobacillin with aspergillus niger protoplast membrane, the site of its action. | mycobacillin partially quenched the strong fluorescence when 1-anilino naphthalene 8-sulfonate (ans) was added to protoplast or plasma membrane but is without any effect on weak fluorescence when added to cell-free extract. there are two classes of ans binding sites on protoplast or plasma membrane of which one class is sensitive to mycobacillin, being competitively abolished by it. mycobacillin also non-competitively inhibits the binding of pyrene, a lipid specific probe. thus it follows from t ... | 1987 | 3624066 |
| mansonella perstans microfilaraemia among the bori community in the niger delta area of nigeria. | night and day blood surveys for filariasis were conducted among inhabitants of bori local government area in the niger delta area of nigeria between october 1983 and july 1984. of the 1674 persons examined 47.0% had microfilaraemia. the infections were due to mansonella perstans only. the prevalence of the infection was higher among males (51.8%) than among females (42.4%), and increased with age in both sexes. eight persons were found with elephantoid scrotums and all had microfilariae of m. pe ... | 1986 | 3632101 |
| biotyping of micelial fungus cultures by the killer system. | based on the occurrence of the yeast killer phenomenon in hyphomycetes, the toxic effect of 37 selected killer yeasts was studied on eleven strains of pseudallescheria boydii, six strains of aspergillus niger, 18 strains of penicillium camemberti and nine strains of sporothrix schenckii. the demonstration of different biotypes within the species of p. boydii and p. camemberti proves that the killer system also is a practical and effective method for epidemiological studies among hyphomycetes. ba ... | 1987 | 3653352 |
| lipid composition and amino acid transport in a nystatin-resistant mutant of aspergillus niger. | a nystatin-resistant mutant of aspergillus niger has been isolated and used as a model system to study the effect of altered sterol levels on lipid composition, transport behavior and physical properties of membrane lipids. there is a decrease in the sterol to phospholipid ratio in the mutant compared to the wild type. although there is no qualitative change in phospholipid composition, the mutant contains a higher amount of phosphatidylcholine and a lower amount of phosphatidylethanolamine comp ... | 1987 | 3669923 |
| mycoflora and trichothecene toxins of paddy grains from egypt. | 120 species and 38 genera were collected from 64 samples of paddy grains on glucose- and cellulose-czapek's agar at 28 degrees c. the total count of glycophilic and cellulose-decomposing fungi fluctuated between 216-29760; and 124-11320 colonies/g paddy grains on the two media, respectively. on glucose agar, the most common species were aspergillus niger, a. flavus, a. sydowi, a. terreus, a. fumigatus, a. ochraceus, penicillium chrysogenum, p. corylophilum, fusarium oxysporum, alternaria alterna ... | 1987 | 3696188 |
| intestinal parasitic infections in pre-school children in the niger delta. | stool specimens from 1,062 pre-school children in the niger delta were examined for the presence of intestinal parasites. of that number, 77.6% harboured one or more than one species of intestinal parasites. ascaris lumbricoides (51.9%) and trichuris trichiura (41.7%) were the most prevalent species recorded. other parasites harboured by these children included hymenolepis sp. (18.3%), giardia lamblia (9.2%), entamoeba histolytica (4.6%), strongyloides stercoralis (1.4%). hookworm, taenia sp., i ... | 1986 | 3701056 |
| fungitoxic effects of nonprotein imino acids on growth of saprophytic fungi isolated from the leaf surface of calliandra haematocephala. | four saprophytic and pathogenic fungi were isolated from the leaf surface of calliandra haematocephala, a tropical legume known to contain large amounts of rare nonprotein imino acids in its leaves and seeds. the fungi aspergillus niger, aspergillus sp., curvularia sp., and penicillium sp. were cultured in the laboratory and tested for susceptibility to leaf extracts of the host plant and to proline, pipecolic acid, cis-5-hydroxypipecolic acid, and 2,4-trans-4,5-cis-4,5-dihydroxypipecolic acid. ... | 1986 | 3707119 |
| separation of mycotoxin-containing sources in grain dust and determination of their mycotoxin potential. | two distinct reservoirs of mycotoxins exist in fungal-infected cereal grains--the fungal spores and the spore-free mycelium-substrate matrix. many fungal spores are of respirable size and the mycelium-substrate matrix can be pulverized to form particles of respirable size during routine handling of grain. in order to determine the contribution of each source to the level of mycotoxin contamination of dust, we developed techniques to harvest and separate mycelium-substrate matrices from spores of ... | 1986 | 3709472 |
| selective action of mycobacillin on the cellular permeability of aspergillus niger. | cells of aspergillus niger normally release not all but of some specific cell constituents viz., lysine, proline, atp, pi, na+, k+ and ca2+ in the absence of mycobacillin. mycobacillin enhances the release of these materials without causing lysis. the time and concentration of mycobacillin for optimum release depends on the nature of the materials involved. | 1986 | 3710916 |
| aspergillus sinusitis in cancer patients. | paranasal sinusitis occurred in 52 immunosuppressed cancer patients treated over 5 years at the university of maryland cancer center. twenty-one patients had aspergillus sinusitis; aspergillus sp, including flavus and niger were directly recovered from sinus in 19 of the 21 infections. two other patients with sinus involvement and positive nose cultures for aspergillus flavus or fumigatus and microbiologically documented pulmonary aspergillosis were considered clinically, although not microbiolo ... | 1986 | 3719529 |
| [disseminated cryptococcosis in 2 aids patients. a contribution to cryptococcosis diagnosis in aids]. | in two aids patients (homosexual men) microscopical demonstration of cryptococcus neoformans in samples obtained by puncture of the liver (n = 1) and additionally of the spleen (n = 1) led to the diagnosis of systemic cryptococcosis. using the india ink method capsulated cryptococcus neoformans cells could also be detected in cerebrospinal fluid (csf) and urine. concomitant culture of the fungus from tracheal secretion, csf, urine and faeces confirmed the diagnosis of a disseminated infection; t ... | 1986 | 3720562 |
| prevalence of toxoplasma gondii among west african rodent, thryonomys swinderianus from the niger delta. | the prevalence of toxoplasma gondii among west african rodent, thryonomys swinderianus, was assessed by the sabin-feldman dye-test. all of the 104 animals examined in the study were seropositive for toxoplasma dye-test antibody. this indicates that there exists among these rodents a considerable reservoir of toxoplasma from which infection can be transmitted to man and other animal species in that area. | 1986 | 3722813 |
| the kinetics of the autolytic phase of growth in cultures of aspergillus niger. | the kinetics of the autolytic phase of growth in cultures of aspergillus niger has been studied. two different autolytic periods could be distinguished. one, consisting of a rapid (exponential) loss (62%) of mycelial weight, occurred between 36 and 117 hours of incubation. a second, consisting of a slow autolysis, occurred between the 117th and the 190th hour of incubation; the mycelial loss here being 5%. based on the degree of autolysis (alpha = 67.0%), 92.5% and 7.5% are lost during the first ... | 1986 | 3724837 |
| inhibitory activity of the genus chrysosporium in relation to the composition of the culture media used. | the effect of nutrients on the formation and accumulation of secondary metabolites in species of the genus chrysosporium was examined. the study was performed according to the method of campbell (1960), and among the results obtained was the fact that the only significant increase in antimicrobial capacity was observed when the strains were grown on a 2% malt extract agar and when micro-organisms were assayed using aspergillus niger. | 1986 | 3736442 |
| degradation of orcinol by aspergillus niger. | aspergillus niger could utilize orcinol (5-methyl-resorcinol or 3,5-dihydroxytoluene) as the sole source of carbon and energy. in the first step of catabolism a. niger hydroxylates orcinol to form 2,3,5-trihydroxytoluene. its oxidized form, 2-hydroxy-6-methyl-1,4-benzoquinone, was also formed in the culture medium during growth of this organism. orcinol-grown cells showed a net increase in the intracellular acetate pool, compared with glucose-grown cells. cell-free extracts of orcinol-grown cell ... | 1986 | 3742332 |
| production and characterization of protein fractions isolated from biomass of aspergillus niger a-3. | | 1986 | 3748121 |
| nadp-specific isocitrate dehydrogenase from the citric acid-accumulating fungus aspergillus niger. | nadp-specific isocitrate dehydrogenase [threo-ds-isocitrate: nadp+ oxidoreductase (decarboxylating), ec 1.1.1.42] was purified 200-300-fold from the citric acid-accumulating fungus aspergillus niger. the enzyme consists of a single polypeptide chain with a molecular mass of 60 +/- 4 kda and has a pi of 5.9 +/- 0.2. only a single enzyme protein was found, although the enzyme appears to occur both in the mitochondrion and in the cytoplasm. growth on organic acids as carbon sources or on no3- as ni ... | 1986 | 3753466 |
| glyceride synthesis by a cell-bound lipase from aspergillus niger. | a cell-bound lipase from the mould aspergillus niger was isolated and purified to homogeneity as judged by disc electrophoresis on a sephadex g-75 column. molecular weight determination and electrophoretic mobility (rf value) indicated that the enzyme differed from that secreted extracellularly. the cell-bound lipase showed a higher capacity than the extracellular enzyme for glyceride synthesis, and reacted in an opposite manner in the synthetic reaction compared with the extracellular enzyme. | 1986 | 3762422 |
| pulmonary aspergillosis and the importance of oxalate crystal recognition in cytology specimens. | a 62-year-old man, previously healthy but alcoholic, and who was clinically thought to have bacterial pneumonia, presented with a pulmonary infiltrate in the right apex, and suddenly died of exsanguinating hemoptysis. sputum cultures yielded aspergillus niger and candida krusei while sputum cytology revealed numerous birefringent crystals in a background of acute inflammatory exudate. autopsy findings showed invasive aspergillosis with a large mycetoma-containing cavity in the lung that was asso ... | 1986 | 3778147 |
| construction of glucose oxidase-loaded human erythrocytes: a model of oxidative cytotoxicity. | human red blood cells were loaded with glucose oxidase from aspergillus niger by a standardized procedure of encapsulation involving transient hypotonic hemolysis followed by isotonic resealing. the amount of loaded enzyme activity, as evaluated by o2 consumption at 5 mm glucose, ranged from 40 to 75 mumoles o2/hr/ml of packed red cells at 37 degrees c. the red cells loaded with glucose oxidase were found to behave as efficient glucose-consuming bioreactors. moreover, at 5 mm glucose, no clear m ... | 1986 | 3804702 |
| purification and some properties of three serine carboxypeptidases from aspergillus niger. | three enzymes exhibiting peptidyl-l-amino acid hydrolase and esterase activities have been purified by immobilized metal-ion affinity chromatography and ion-exchange chromatography. the three enzymes were entirely free of the acid protease activity that normally exists along with them in the crude culture filtrates of aspergillus niger. although all three exo-peptidases possessed nearly identical molecular weights (ca. 140,000), isoelectric points (ca. 5.0) and other properties, their affinities ... | 1986 | 3805224 |
| [complications of measles in adult africans. apropos of 95 cases]. | 85 cases of measles with complications have been reported in agades (niger) from september 1983 to march 1985. the patients were all adults more than 15 years of age. such complications are more frequent during winter season. the disease is superposable to the one observed in children: same course, same types of complications (superinfection, undernutrition, dehydration, broken compensation of a parasitosis, encephalitis). mortality rate is next to the one observed in child (18.2%). death occurs ... | 1986 | 3807754 |
| [a case of hydatid cyst of the mediastinum in the republic of niger]. | the authors report on an observation made at the niamey hospital, because of the combined infrequency of both human hydatidosis in western africa and mediastinal localization of the observed cases. | 1986 | 3807759 |
| [effectiveness of an amphoteric surface agent against schistosoma mansoni miracidia in field-like conditions in niger]. | the laurylamidopropylbétaïne, a surface active substance, has been tested in field conditions, in niger, against schistosoma mansoni miracidia. experiments were carried out in artificial ponds of 20 m3, in which conditions were similar, as far as possible, to conditions found in transmission sites. the product showed a satisfactory remanence and the percentage of infected molluscs dropped down to zero with a low (2 ppm) concentration. it is suggested that laurylamidopropylbetaïne could be used i ... | 1986 | 3813413 |
| isolation and characterization of an unsaturated fatty acid requiring mutant (ufa4) of aspergillus niger. | | 1986 | 3817878 |
| [changes in the activity of krebs cycle enzymes in an aspergillus niger strain during the biosynthesis of citric acid]. | | 1985 | 3834769 |
| [intracellular appearance, morphological feature and properties of aspergillus niger virus]. | | 1985 | 3837544 |
| antibacterial and antifungal activity of avarone and avarol. | the sesquiterpenoid hydroquinone and quinone, avarol and avarone, were previously found to be potent antitumor agents (müller et al., 1984). in the present study it is reported that in aqueous solution (ph 7.2), in the presence of dimethylsulfoxide, avarol is converted to avarone. avarone and to a smaller extent also avarol were active against a variety of grampositive bacterial species. the highest activity was determined for streptococcus pneumoniae and erysipelothrix rhusiopathiae (mic 0.781 ... | 1985 | 3841441 |
| primary cutaneous aspergillosis in six leukemic children. | we report a cluster of primary cutaneous aspergillosis in six children with hematologic malignancy. when first seen, they had hemorrhagic bullae caused by aspergillus flavus, aspergillus fumigatus, and aspergillus niger at the sites of insertion of intravenous cannulas or where arm boards had been taped to the extremities. rapid diagnosis of cutaneous aspergillosis was made by direct examination of the blister roof with potassium hydroxide before it progressed to a necrotic ulcer. intravenous am ... | 1985 | 3855880 |
| crystals occurring in pulmonary cytology specimens. association with aspergillus infection. | birefringent needlelike crystals in rosette or wheat-sheaf-like arrangements were found in pulmonary cytology specimens from 11 of 65 patients who had either sputum cultures positive for aspergillus or histologically confirmed pulmonary aspergilloma. no crystals were found in specimens from 60 control patients with and without known fungal disease. the crystals were most often associated with a. niger infection (45.4%), followed by a. flavus (16%). crystals were also observed in one case of a. f ... | 1985 | 3863422 |
| the epidemiology of echinococcus infection in the niger delta. | | 1985 | 3873083 |
| dual-parameter scatter-flow immunofluorescence analysis of bacillus spores. | using a commercial flow cytometer (cyto-fluorograf), narrow-forward-angle (nfa) light-scatter signals were detected for spore preparations of bacillus anthracis vollum, b. anthracis sterne, b. cereus nctc 8035, and b. subtilis var niger. in the flow immunofluorescence (fif) analysis of spores stained with fluorescein-conjugated hyperimmune antibody to b. anthracis vollum spores, fluorescence histograms could be acquired by selecting on nfa scatter. fluorescence data selected on ninety degree sca ... | 1985 | 3884295 |
| transformation of aspergillus niger by the amds gene of aspergillus nidulans. | aspergillus niger grows poorly on acetamide as a nitrogen or carbon source and lacks sequences detectably homologous to the amds gene encoding the acetamidase of aspergillus nidulans. we have taken advantage of these observations to develop a transformation system for a. niger using the amds gene as a dominant heterologous marker for selecting transformants on the basis of acetamide utilization. transformants varied in their ability to grow on amide media and the number of integrated copies of t ... | 1985 | 3894007 |
| purification of an acid protease and a serine carboxypeptidase from aspergillus niger using metal-chelate affinity chromatography. | | 1985 | 3894397 |
| sampling and isolation of cryptococcus neoformans from indoor air with the aid of the reuter centrifugal sampler (rcs) and guizotia abyssinica creatinine agar. a contribution to the mycological-epidemiological control of cr. neoformans in the fecal matter of caged birds. | in february and march 1984, cryptococcus neoformans was detected in the manure and ambient air of a volery in the berlin zoo in which a palm cockatoo (probosciger aterrimus) was kept. both times, 5 colony-forming units of cr. neoformans could be isolated on the differential medium, guizotia abyssinica creatinine agar, from 40 1 of air aspirated by the reuter centrifugal sampler (rcs). the absence of a concurrent growth of moulds was found to be of special importance for the optimal isolation of ... | 1985 | 3895777 |
| transformation of aspergillus niger using the argb gene of aspergillus nidulans. | a mutant of aspergillus niger defective in ornithine transcarbamylase function was transformed with plasmids carrying a functional copy of the argb gene of aspergillus nidulans after treatment of spheroplasts in the presence of polyethylene glycol and calcium ions. the plasmid pdg3 gave stable transformants at a frequency of 4 per microgram of input dna. southern blot analysis of dna from transformants showed that pdg3 dna had integrated into the a. niger chromosomes at a variety of locations. t ... | 1985 | 3902571 |
| [farmer's lung with antibodies against thermoactinomyces vulgaris and aspergillus fumigatus. clinical course and treatment]. | we present a case of farmer's lung with antibodies to thermoactinomyces vulgaris and aspergillus fumigatus. a 56-year-old male patient with an atopic family medical history came to our hospital complaining of cough, dyspnea, fever, asthenia and anorexia. his condition worsened after being exposed to cereal powder, becoming symptomatic after 20 minutes or on occasion after 6 hours. physical examination showed basal crepitant rales in lung auscultation. the radiograph of the thorax showed a bilate ... | 1985 | 3909794 |
| a study of some species in the aspergillus niger group. | | 1985 | 3914613 |
| a cryptococcus neoformans strain from the brain of a wildlife fox (vulpes vulpes) suspected of rabies: mycological observations and comments. | a cryptococcus neoformans strain which in 1983 caused an infection of the central nervous system (cns) in a wildlife fox with rabies-like symptoms was tested for its strain-specific brown colour effect (bce) on guizotia abyssinica creatinine agar and for its ability to assimilate creatinine. both reactions were found to be positive. these results were found to be largely identical with those of 2 out of 3 strains having caused fatal cryptococcosis in 1970, 1979 and 1983 in persons living in the ... | 1985 | 3914796 |
| effective in vivo hydrolysis of milk lactose by beta-galactosidases in the presence of solid foods. | the feasibility of enzyme replacement therapy with exogenous, food-grade, microbial enzymes at mealtime to effect intragastrointestinal hydrolysis of the lactose from 360 ml of cow's milk consumed with a solid food meal (breakfast cereals) was investigated in adult guatemalan lactose-malabsorbers using a hydrogen breath-analysis procedure to quantify the completeness of postprandial carbohydrate absorption. adding 2 g of a commercial preparation of beta-galactosidase from kluyveromyces lactis at ... | 1985 | 3918431 |
| antimicrobial activities of n-chloramines and diazolidinyl urea. | a combination of mics of an n-chloramine, a simple chlorinated amino acid, and diazolidinyl urea gave synergistic activity against bacteria, but not fungi. the two compounds at a higher concentration, 0.1 and 0.3%, respectively, gave synergistic inhibition of fungi; kill times were 1 h for trichophyton tonsurans, 3 h for aspergillus niger and fusarium moniliforme, and 6 h for aspergillus fumigatus. | 1985 | 3920962 |
| antigens of aspergillus fumigatus. iii. comparative immunochemical analyses of clinically relevant aspergilli and related fungal taxa. | cell sap (cs) of the pathogenic fungus aspergillus fumigatus strain ag-507 was fractionated by sephadex g-200 column chromatography. a protein fraction designated cs3 was partially characterized by two dimensional electrophoresis (2-de) and analytical ultracentrifugation. cs3 consisted mainly of low molecular weight components (14 k-43 k) of the whole cs, and produced one peak in analytical ultracentrifugation with an sapp of 4.25. cs3 was demonstrated to be different from a previously character ... | 1985 | 3921301 |
| [isolation of a highly purified glucoamylase from aspergillus niger 16/132]. | | 1985 | 3929568 |
| [biosynthesis of glucoamylase and the possibilities of using it for the conversion of starch into sugar. i. the effect of the type and concentration of the carbon source on glucoamylase biosynthesis by aspergillus niger strain b 77]. | | 1985 | 3929569 |
| [effect of the homogeneity of the culture, age and amount of the inoculation matter on glucoamylase biosynthesis by aspergillus niger strain b 77]. | | 1985 | 3929570 |
| isolation and properties of beta-glucosidase from aspergillus niger. | | 1985 | 3934065 |
| variation of a 470 000 daltons antigen complex and catalase antigen in clinical isolates of aspergillus fumigatus. | antigens in ruptured mycelium of 18 aspergillus strains including 14 clinical isolates of a. fumigatus were studied by immunoelectrophoresis. one antigenic component of molecular weight 470 000 previously characterized by hydrophobic interaction chromatography and gel filtration and a second component with catalase activity were detected in all a. fumigatus isolates but in varying quantities. the 470 000 antigen complex cross-reacted with antigens in a. flavus and a. nidulans but not in a. niger ... | 1985 | 3934773 |
| evidence that the mechanisms of fungitoxicity of 8-quinolinol and its bischelate with copper(ii) are different. | the copper(ii) contents of the growth media, sabouraud dextrose and czapek-dox broths, and of the spore inocula of aspergillus niger (atcc 1004), aspergillus oryzae (atcc 1011), trichoderma viride (atcc 8678), and myrothecium verrucaria (atcc 9095) were determined by atomic absorption spectrophotometry in a graphite furnace. the test systems composed of sabouraud dextrose broth and spore inocula of the four fungi contained only a little over 3% of the copper(ii) required to form a minimal inhibi ... | 1985 | 3935305 |
| mycotoxins producing fungi and mycoflora of air-dust from taif, saudi arabia. | using the dilution plate method, 70 species and 31 genera were collected from 20 dust samples on glucose (28 genera and 64 species) and cellulose czapek's agar (22 genera and 46 species) at 28 degrees c. the most common fungi were aspergillus niger, a. flavus, a. flavus var. columnaris, phoma glomerata, fusarium oxysporum, penicillium chrysogenum and mucor racemosus; and a. nidulans, phoma humicola, drechslera spicifera and stachybotrys chartarum on the two media, respectively. toxicity test sho ... | 1985 | 3935928 |
| [urinary bilharziasis and sideropenic anemia. study of their relation in 2 villages in niger]. | in order to assess schistosoma haematobium as an aetiologic factor in sideropenic anaemias, we compared a village with high prevalence of urinary schistosomiasis and a village without urinary schistosomiasis as a control. in the infested village, anaemias (haemoglobin value lower than 12 g/dl) especially affects children between 5 to 14 years old (71%:45/68). boys being the most infestated group with s. haematobium (prevalence:100%; mean egg output: 496 eggs/10 ml of urine) are also the most ana ... | 1985 | 3936632 |
| purification and properties of an alpha-d-xylosidase from aspergillus niger. | two components of alpha-d-xylosidase (alpha-d-xylosidase i and ii) were detected in the culture filtrate of aspergillus nigher grown in a medium containing sanzyme 1000-treated glyloid 2a. the major component (alpha-d-xylosidase i) was purified to an electrophoretically pure state. the purified enzyme showed approximately 540-fold increase in specific activity over the original culture filtrate. the purified enzyme was shown to be an oligomeric protein consisting of four subunits, each of which ... | 1985 | 3936844 |
| spore heat resistance and specific mineralization. | spores of bacillus megaterium atcc 19213, bacillus subtilis niger and bacillus stearothermophilus atcc 7953 were converted to fully demineralized, but viable, h forms by controlled acid titration. h forms were more heat sensitive than were native forms, but z values were greater for killing of h spores than those for native spores. therefore, the differences in heat sensitivity between native and h forms decreased with increasing killing temperature. the increase in heat sensitivity associated w ... | 1985 | 3937495 |
| investigations on the sporicidal and fungicidal activity of disinfectants. | the sporicidal and fungicidal activity of disinfectants was studied in a suspension test. glutaraldehyde 4%, sodium-dichloroisocyanurate-dihydrate (2400 ppm active chlorine) and peracetic acid 0.25% demonstrated after 30 min of exposure at 20 degrees c in the presence of 4% horse serum a clear activity against spores of bacillus cereus. under the same conditions formaldehyde 4% and glutaraldehyde 2% were also found to be sporicidal, but only after a longer time of exposure. spores of bacillus an ... | 1985 | 3938146 |
| [prevalence of penicillinase-producing neisseria gonorrhoeae and treatment of gonorrhea in niger]. | | 1985 | 3938201 |
| occupational asthma caused by cellulase. | inhalation of enzyme dusts can cause occupational asthma, and the list of enzymes able to induce asthma is increasing. we report two patients with asthma induced by occupational exposure to cellulase powder derived from aspergillus niger. a type i hypersensitivity to this enzyme was demonstrated by means of skin test reactivity, positive passive transfer test, positive reverse enzyme immunoassay for specific ige, and immediate bronchial provocation test response to cellulase dust. skin tests wit ... | 1986 | 3958392 |
| [seasonal variation of allergy-causing fungi in the southern part of mexico city]. | the presence of mycotic flora in atmospheric medium has always been an interesting field for allergologists and ecologists interested in the problems of environmental contamination. in 1904 saito in japan demonstrated that certain spores of fungus when inhaled by susceptible individuals can produce severe respiratory processes. since then many studies have been performed to determine the generic and species of causative fungus responsible for respiratory allergy, as well to understand its growth ... | 1986 | 3962816 |
| hydration of cellobial by exo- and endo-type cellulases: evidence for catalytic flexibility of glycosylases. | new insight has been obtained into the catalytic capabilities of cellulase. essentially homogeneous preparations of exo- (or avicelase-) type and endo- (or cmcase-) type cellulases from irpex lacteus and aspergillus niger, respectively, were shown to hydrate the enolic bond of cellobial to form 2-deoxycellobiose. the a. niger enzyme also synthesized a small amount of a 2-deoxycellobiosyl-transfer product from cellobial. by use of digests conducted in deuterated buffer and 1h nmr spectra for prod ... | 1986 | 3964662 |
| presence and regulation of the alpha-ketoglutarate dehydrogenase multienzyme complex in the filamentous fungus aspergillus niger. | alpha-ketoglutarate dehydrogenase has been demonstrated for the first time in cell extracts from the filamentous fungus aspergillus niger. a minimum protein concentration of 5 mg/ml is necessary for detecting enzyme activity, but a maximum of ca. 0.060 mumol/min per mg of protein is observed only when the protein concentration is above 9 mg/ml. alpha-ketoglutarate can partly stabilize the enzyme against dilution in the assay system. neither bovine serum albumin nor a variety of substrates or eff ... | 1985 | 3968029 |
| mineralization and heat resistance of bacterial spores. | the heat resistances of the fully demineralized h-form spores of bacillus megaterium atcc 19213, b. subtilis var. niger, and b. stearothermophilus atcc 7953 were compared with those of vegetative cells and native spores to assess the components of resistance due to the mineral-free spore state, presumably mainly from dehydration of the spore core, and to mineralization. mineralization greatly increased heat resistance at lower killing temperatures but appeared to have much less effect at higher ... | 1985 | 3968042 |
| antimicrobial activity of methyl esters and nitriles of 2-cyano-3-(5'-r-2'-furyl)propenic acid. | derivatives of 2-cyano-3-(2'-furyl)propenic acid with a markedly polarized double bond inhibit the growth of chlorella pyrenoidosa, saccharomyces cerevisiae, candida albicans and aspergillus niger at concentrations above 40 mumol/l. their antibacterial activity (escherichia coli b, bacillus subtilis) is low. the biological effect increases with an increasing electron acceptor effect and decreasing hydrophobicity of the substituent on the furan ring. substitution of methoxycarbony] group with cya ... | 1985 | 3979924 |
| fungal flora of egyptian baladi bread with special reference to the mutagenic effects of their toxic metabolites. | the fungal flora of wheat flour and baladi bread in upper egypt were investigated. most of the isolated fungal species belong to the genus aspergillus. the presence of non heat resistant fungi of the both flat surfaces of baladi bread, came from contamination after baking and from improper handling at homes. among the heat resistant fungi, a. fumigatus and a. niger, were recorded to inhabit the spongy crumb although the high temperature of baking process which reached approximately 100 degrees c ... | 1985 | 3982490 |
| clinical and immunological reactions to aspergillus niger among workers at a biotechnology plant. | the workforce at a biotechnology plant producing citric acid by fermentation of molasses with a strain of aspergillus niger was studied. a combination of a respiratory questionnaire and clinical assessment identified 18 subjects (4.9% of the workforce) with work related bronchospasm. in nine of these evidence of sensitisation to a niger was obtained by skin prick tests and radioallergosorbent test (rast) using as an antigen an extract of the a niger culture fluid from the process. of the 325 sub ... | 1985 | 3986142 |
| [schistosoma haematobium bilharziosis and urinary infections. study of their relationship in 2 villages of niger]. | in order to assess the relation between urinary schistosomiasis and urinary tract infections (u.t.t.), a study of two villages in the surroundings of niamey (rep. of niger) was undertaken. in the first village located near a rice plantation irrigation scheme, the global prevalence of s. haematobium infection was 77%. boys between 5 to 14 years of age were all infected and their mean egg output was 500 eggs/10 ml of urine. in the second village, where water is supplied from wells, schistosomiasis ... | 1985 | 3986954 |
| the effect of ketoconazole on amphotericin b in a model of disseminated aspergillosis. | the potential of ketoconazole prophylaxis to antagonize the activity of amphotericin b against aspergilli was investigated in vitro and in neutropenic mice. exposure of aspergillus fumigatus (six strains) and of aspergillus flavus or aspergillus niger to ketoconazole resulted in a uniform increase of the minimal fungicidal activity of amphotericin b, from 0.15-0.63 mg/liter to greater than 2.5 mg/liter in a microwell assay. to test the relevance of this antagonism in vivo, we challenged neutrope ... | 1985 | 3989324 |
| [the distribution of free and esterified carboxyl groups within the pectin molecule after the action of pectin esterase from aspergillus niger and oranges]. | by reaction of pectin esterase (pe) from aspergillus niger and oranges as well as lye, with 95% esterified citrus and apple pectin we prepared series of preparations with degrees of esterification between 35 and 77%. in these partial deesterified pectins the form of distribution of the free and esterified carboxyl groups has been determined from the activity coefficient gamma ca2+ of the calcium counterions in the solutions of the corresponding calcium pectinates, from the electrostatic free ent ... | 1985 | 3990779 |
| prevalence of hydatid cysts in domestic livestock in the niger delta. | the prevalence of infection with hydatid cysts in pigs, cattle, sheep and goats in the niger delta area of nigeria studied over a 10-month period was generally high; 56% in pigs, 42% in goats, 32% in cattle and 24% in sheep. this high prevalence rate is probably related to the socio-economic factors prevalent in the area. the high prevalence and viability of the cysts in pigs and cattle suggests that the strain of echinococcus present in this area differs from those reported elsewhere. | 1985 | 3992669 |
| liberation of electrolytes during the induced autolysis of aspergillus niger. | the induced autolysis of aspergillus niger was studied by suspending mycelia in water; after 120 hours there was a 50% reduction in dry weight. the kinetics of the liberation of electrolytes during autolysis was studied, and a positive correlation was observed between the electrical conductivity of the autolizate and autolysis loss of mycelium dry weight. | 1985 | 3993252 |
| bacterial and fungal cometabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) and its breakdown products. | resting cells of bacteria grown in the presence of diphenylmethane oxidized substituted analogs such as 4-hydroxydiphenylmethane, bis(4-hydroxyphenyl)methane, bis(4-chlorophenyl)methane (ddm), benzhydrol, and 4,4'-dichlorobenzhydrol. resting cells of bacteria grown with benzhydrol as the sole carbon source oxidized substituted benzhydrols such as 4-chlorobenzhydrol, 4,4'-dichlorobenzhydrol, and other metabolites of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt), such as ddm and bis(4-chloro ... | 1985 | 3994362 |
| biological activity of 4-hydroxyisophthalic acid derivatives. iii. variously substituted anilides with antimicrobial activity. | a series of 1,3-bis-anilides of 4-hydroxyisophthalic acid was prepared and investigated for antibacterial and antifungal activities. the prepared compounds (i-xiv), of the general formula (a), where xn = 2-no2 (i); 2,4-(no2)2 (ii); 2,4-no2, cl (iii); 2,4-no2,cf3 (iv); 3,4-no2,cl (v); 2,4-cl,no2 (vi); 2,5-cl,no2 (vii); 2,4,6-cl,no2,cl (viii); 2,4-br, no2 (ix); 2-cf3 (x); 3-cf3 (xi); 2,5-cl,cf3 (xii); 2,5-ch3,cl (xiii); 3,4-cl,ch3 (xiv), were obtained in satisfactory yield by reacting 4-hydroxyiso ... | 1985 | 3994839 |
| [effect of ozone on the growth and development of lower fungi (phytophora infestans, aspergillus niger)]. | | 1985 | 3996196 |
| pseudomembranous necrotizing bronchial aspergillosis. a variant of invasive aspergillosis in a patient with hemophilia and acquired immune deficiency syndrome. | a 15-yr-old male hemophiliac developed the acquired immune deficiency syndrome (aids). his terminal illness was characterized by rapidly progressive respiratory failure with intermittent wheezing, nonresponsive to bronchodilator and steroid therapy. postmortem examination revealed a pseudomembrane covering the mucosa of the lower trachea and bronchi of both lungs. this pseudomembrane was composed predominantly of fungal hyphae speciated as aspergillus niger. there was widespread transmural necro ... | 1985 | 4003949 |
| metabolic effects of manganese deficiency in aspergillus niger: evidence for increased protein degradation. | the effect of manganese deficiency on macromolecule synthesis has been studied in a citric acid producing strain of aspergillus niger: pulse labelling experiments showed that the synthesis of both protein and rna was not influenced by the presence of manganese; however, increased protein degradation occurred under manganese deficiency. this was also reflected by the increased activity of an intracellular proteinase activity under these conditions. in replacement cultures addition of inhibitors o ... | 1985 | 4004449 |
| an unsaturated fatty acid mutant of aspergillus niger with partially defective delta 9-desaturase. | the wild-type aspergillus niger (v35) does not require fatty acids for growth. four unsaturated fatty acid auxotrophs designated as ufa1, ufa2, ufa3, and ufa4 have been produced from this organism by treating the conidia of the wild-type strain with a mutagen, n-methyl-n'-nitro-n-nitrosoguanidine, followed by isolation on media containing monounsaturated fatty acids and the nonionic detergent, brij 58. optimal growth of the mutants comparable with that of the wild type was achieved with medium s ... | 1985 | 4005715 |
| lipid profiles of aspergillus niger and its unsaturated fatty acid auxotroph, ufa2. | a comparative study of the mycelial lipid composition of a wild strain (v35) and one unsaturated fatty acid auxotroph (ufa2) of aspergillus niger has been performed. the lipid composition of both strains are qualitatively the same but quantitatively different. all the strains contain the following phospholipids: cardiolipin, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylcholine, and phosphatidylserine; and triglycerides, diglycerides, monoglycerides ... | 1985 | 4005716 |
| zn2+-induced cooperativity of mannitol-1-phosphate dehydrogenase from aspergillus parasiticus. | mannitol-1-phosphate dehydrogenase (ec 1.1.1.17) has been purified from aspergillus parasiticus, a filamentous fungus which produces the polyketide mycotoxin, versicolorin a. its kinetic properties have been compared with those of mannitol-1-phosphate dehydrogenase from the related non-toxin-producing fungus, a. niger. both enzymes are inhibited by divalent transition metals, especially zn2+ and cd2+, but only the enzyme from a. parasiticus exhibits inhibitor-induced cooperative binding of the s ... | 1985 | 4019499 |
| antifungal properties of 3-n-alkyn-1-ols and synergism with 2-n-alkyn-1-ols and ketoconazole. | twelve 3-n-alkyn-1-ols (c4-c12, c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by ph of the medium, and significantly ... | 1985 | 4020633 |
| hydroxylation of benzoate and its chlorinated derivatives in aspergillus niger. | comparison of the properties of a purified benzoate-4-hydroxylase, using benzoic acid and its chlorinated derivatives as substrate, suggests that the same enzyme operates during the initial hydroxylation of benzoate, 2-chlorobenzoate (2-cba), and 3-chlorobenzoate (3-cba) in a.niger. | 1985 | 4026866 |
| [2 cases of hydatidosis in the republic of niger]. | two cases of hydatidosis have been observed at the hospital of niamey, one involving the tibia and the other one the liver and the spleen. for unknown epidemiological factors, this helminthiasis is rare in man in western africa. | 1985 | 4028311 |
| a note on crossing experiments with aspergillus niger for the production of calcium gluconate. | a strong calcium gluconate-producing strain of aspergillus niger (mn181) was obtained by way of mutagenic treatment. its growth was very slow with moderate sporulation. the strain was treated with n-methyl-n'nitro-n-nitrosoguanidine (mnng) and some auxotrophic mutants were obtained. all were less productive than the parent strain in producing calcium gluconate. the reduced yield was corrected in the heterokaryons and diploids derived by crossing sister strains. one diploid strain (d4), heterozyg ... | 1985 | 4030528 |