| optimization of bioethanol production during simultaneous saccharification and fermentation in very high-gravity cassava mash. | hydrolysis and fermentation conditions for production of ethanol from very high-gravity cassava mash by saccharomyces cerevisiae during simultaneous saccharification and fermentation (ssf) processing were optimized using a statistical methodology. during the first part of the study, placket-burman design (pbd) was used to study 19 factors that could potentially influence ethanol production. gravity, particle size, initial ph, and fermentation temperature were identified as key factors that signi ... | 2011 | 20803106 |
| brain glutathione as a target for aetiological factors in neurolathyrism and konzo. | both neurolathyrism and konzo are associated with the nutritional dependence of human populations on a single plant food. these diseases express themselves as chronic disorders of upper motor neurones, leading to signs and symptoms that characterise amyotrophic lateral sclerosis (motor neurone disease). the plant food associated with neurolathyrism is grass pea, which contains the neurotoxic β-n-oxalyl-α,β-diaminopropionic acid (β-odap). the plant food associated with konzo is cassava, which may ... | 2011 | 20816718 |
| cryopreservation of sweetpotato (ipomoea batatas) and its pathogen eradication by cryotherapy. | sweetpotato (ipomoea batatas) ranks as the seventh most important staple crop in the world and the fifth in developing countries after rice, wheat, maize and cassava. sweetpotato is mainly grown in developing countries, which account for more than 95% of total production of the whole world. genetic resources, including cultivated varieties and wild species, are a prerequisite for novel sweetpotato breeding in both conventional and genetic engineering programs. various cryopreservation protocols ... | 2011 | 20851757 |
| fungi immobilization for landfill leachate treatment. | this paper investigated treatment of landfill leachate collected from nonthaburi landfill site, thailand, by using immobilized white rot fungi, namely, trametes versicolor bcc 8725 and flavodon flavus bcc 17421. effects of ph and co-substrates were investigated at different contact times. three types of co-substrates as carbon source used in this study are glucose, corn starch and cassava. treatment efficiency was evaluated based on color, bod, and cod removal. initial bod and cod were found to ... | 2010 | 20861536 |
| provitamin a accumulation in cassava (manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene. | cassava (manihot esculenta) is an important staple crop, especially in the arid tropics. because roots of commercial cassava cultivars contain a limited amount of provitamin a carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin a deficiency disorders. we show here that an allelic polymorphism in one of the two expressed phytoene synthase (psy) genes is capable of enhancing the flux of carbon through car ... | 2010 | 20889914 |
| prodigiosin production by serratia marcescens ucp 1549 using renewable-resources as a low cost substrate. | a new strain of serratia marcescens ucp1459 isolated from a semi-arid soil produced the natural red pigment prodigiosin, characterized by an uncommon pyrrolylpyrromethane skeleton. prodigiosin is a promising drug due to its reported antifungal, immunosuppressive and anti-proliferative activities. the objective of this work was to indentify a suitable medium to simultaneously enhance s. marcescens growth and pigment production using renewable resources obtained from industrial wastes. s. marcesce ... | 2010 | 20938403 |
| digestibility and metabolizable energy values of processed cassava chips for growing and finishing pigs. | determinations of digestibility of dry matter (dm), digestible energy (de), and metabolizable energy (me) in cassava chips with different levels of crude fiber (cf) were measured in growing pigs (20 kg) and finishing pigs (60 kg). the treatments were (1) cassava starch (0% cf), (2) peeled cassava chips (2.5% cf), (3) non-peeled washed cassava chips (3.9% cf), and (4) non-peeled and non-washed cassava chips (5.2% cf). in the growing pigs, peeled cassava chips, non-peeled washed cassava chips, and ... | 2010 | 20938741 |
| soluble fiber dextrins and pullulans vary in extent of hydrolytic digestion in vitro and in energy value and attenuate glycemic and insulinemic responses in dogs. | the objective of this research was to measure in vitro hydrolytic digestion characteristics, glycemic and insulinemic responses, and true metabolizable energy (tme(n)) content of select soluble fiber dextrins (sfds) and pullulans. the sfds were derived either from tapioca starch or from corn starch. the pullulans were of low, intermediate, and high molecular weight. soluble fiber dextrins varied in digestibility, with all substrates resulting in low to intermediate in vitro monosaccharide digest ... | 2010 | 20939499 |
| urine--a valuable fertilizer with low risk after storage in the tropics. | urine contains a considerable amount of nutrients and potentially could be used as supplement commercial mineral fertilizer; however, it carries risk of contamination. the effect of urine storage as a simple treatment method was examined under tropical conditions. initial concentrations of total coliforms (2.3 x 10(5) cfu/100 ml), e. coli (<1.0 x 10(4) cfu/100 ml), and salmonella sp. were less than detection limit after four weeks. open storage led to nitrogen losses of 90%, whereas closed stora ... | 2010 | 20942339 |
| co-culturing of lactobacillus paracasei subsp. paracasei with a lactobacillus delbrueckii subsp. delbrueckii mutant to make high cell density for increased lactate productivity from cassava bagasse hydrolysate. | to increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1:1 ratio of lactobacillus paracasei subsp. paracasei and a fast growing l. delbrueckii subsp. delbrueckii mutant. the culture was embedded on to polyurethane foam (puf) cubes as a biofilm and used for fermentation. in order to prevent the cell leakage, the puf cubes were further entrapped in calcium cross-linked alginate. the maximum lactic acid production using a high cell density free culture was >38 ... | 2010 | 20972788 |
| biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of cyp71e7, the oxime-metabolizing cytochrome p450 enzyme. | cassava (manihot esculenta) is a eudicotyledonous plant that produces the valine- and isoleucine-derived cyanogenic glucosides linamarin and lotaustralin with the corresponding oximes and cyanohydrins as key intermediates. cyp79 enzymes catalyzing amino acid-to-oxime conversion in cyanogenic glucoside biosynthesis are known from several plants including cassava. the enzyme system converting oxime into cyanohydrin has previously only been identified in the monocotyledonous plant great millet (sor ... | 2010 | 21045121 |
| growth performance, carcass characteristics and meat sensory evaluation of west african dwarf sheep fed varying levels of maize and cassava hay. | a study was conducted to determine the growth performance and meat yield and quality of west african dwarf sheep. twenty rams weighing an average of 15.3 ± 0.79 kg live weight and with an average age of 18 months were allotted at random to five dietary treatments of 0%, 25%, 50%, 75% and 100% maize hay (mh) for a period of 105 days. dry matter (dm) intake and growth rate of the rams were improved as the level of cassava hay (ch) increased in the diets. live weight gain varied significantly (p < ... | 2010 | 21082248 |
| effects of streblus asper lour foliage on digestibility, rumen fermentation, and nitrogen balance of growing goats. | eighteen crossbred (thai native x anglo-nubian) goats were chosen from a suranaree university of technology's farm on the basis of similar bodyweight (15.5 ± 3.5 kg). the goats were randomly allocated to three treatments in randomized complete block design. each goat was given urea-treated rice straw as roughage plus the respective treatment diets. the diets were iso-nitrogenous and iso-energetic containing cassava pulp, molasses, urea, and commercial mineral and vitamin mix. the experimental tr ... | 2010 | 21088895 |
| molecular characterization of two distinct monopartite begomoviruses infecting tomato in india. | tomato leaf curl viruses, which are the members of the genus begomovirus, have emerged as devastating pathogens worldwide causing huge economic losses and threatening production of crops like cassava, cotton, grain legumes and vegetables. even though the tolcv isolates from northern india have been shown to possess bipartite genome (designated as dna a and dna b), those from australia, taiwan and southern india have a single genomic component (dna a). we describe here the genetic diversity of tw ... | 2010 | 21092241 |
| production of raw cassava starch-degrading enzyme by penicillium and its use in conversion of raw cassava flour to ethanol. | a newly isolated strain penicillium sp. gxu20 produced a raw starch-degrading enzyme which showed optimum activity towards raw cassava starch at ph 4.5 and 50°c. maximum raw cassava starch-degrading enzyme (rcsde) activity of 20 u/ml was achieved when gxu20 was cultivated under optimized conditions using wheat bran (3.0% w/v) and soybean meal (2.5% w/v) as carbon and nitrogen sources at ph 5.0 and 28°c. this represented about a sixfold increment as compared with the activity obtained under basal ... | 2010 | 21120680 |
| metabolic and transcriptomic responses of weaned pigs induced by different dietary amylose and amylopectin ratio. | starch is one of the major dietary energy sources for mammals. however, the nutritional value of starch largely depends on its amylose and amylopectin ratio. in this study, the overall metabolic and transcriptomic responses of weaned pigs fed with different dietary starches were assessed. sixteen weaned pigs were randomly allotted to two experimental diets containing either of pure cassava starch (cs) or maize starch (ms) as the sole energy source (the amylose-amylopectin ratio were 0.25 and 0.4 ... | 2010 | 21152049 |
| [consumption to feed of resident adult population in rural area of the city of ibatiba (es, brazil)]. | it is a transverse study where a questionnaire of alimentary frequency was applied (qaf) in 150 adults resident of the rural area of the city of ibatiba (es, brazil). qaf classified the alimentary consumption as: habitual (>4 times in the week), not habitual (<4 times in the week) and rarely (1 time a month), with objective of correlating the alimentary consumption with the chronic-degenerative diseases. the results evidenced a habitual consumption of rice, breads, stalk, bean, cow milk, animal ... | 2011 | 21180826 |
| comparative expression of wild-type and highly soluble mutant his103leu of hydroxynitrile lyase from manihot esculenta in prokaryotic and eukaryotic expression systems. | low protein solubility and inclusion body formation represent big challenges in production of recombinant proteins in escherichia coli. we have recently reported functional expression of hydroxynitrile lyase from manihot esculenta, mehnl, in e. coli with high in vivo solubility and activity using directed evolution. as a part of attempts to clarify the mechanism of this phenomenon, we have described the possibility of expression of the highly active and soluble mutant mehnl-his103leu as well as ... | 2010 | 21185385 |
| transient expression of βc1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions. | geminiviruses are emerging plant pathogens that infect a wide variety of crops including cotton, cassava, vegetables, ornamental plants and cereals. the geminivirus disease complex consists of monopartite begomoviruses that require betasatellites for the expression of disease symptoms. these complexes are widespread throughout the old world and cause economically important diseases on several crops. a single protein encoded by betasatellites, termed βc1, is a suppressor of gene silencing, induce ... | 2010 | 21192819 |
| molecular cloning and biochemical characterization of a novel cystatin from hevea rubber latex. | a novel cdna encoding a cysteine proteinase inhibitor or phytocystatin was isolated from hevea brasiliensis rrim600 rubber latex cdna library. the full-length hbcpi obtained from rapid amplification of cdna ends contains 588 bp. an open reading frame of 306 bp encodes for a protein of 101 amino acids with the typical inhibitory motifs of phytocystatin superfamily, namely the central signature motif qxvxg, a gg doublet and larfav-like motifs in the n-terminal part, and conserved a/pw residues in ... | 2010 | 21247772 |
| direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and ß-glucosidase. | in order to develop a method for producing fuel ethanol from cassava pulp using cell surface engineering (arming) technology, an arming yeast co-displaying a-amylase (a-am), glucoamylase, endoglucanase, cellobiohydrase, and ß-glucosidase on the surface of the yeast cells was constructed. the novel yeast strain, possessing the activities of all enzymes, was able to produce ethanol directly from soluble starch, barley ß-glucan, and acid-treated avicel. cassava is a major crop in southeast asia and ... | 2011 | 21327413 |
| genetic variability of east african cassava mosaic cameroon virus under field and controlled environment conditions. | cassava geminiviruses occur in all cassava growing areas of africa and are considered to be the most damaging vector-borne plant pathogens. at least seven species of these viruses have been identified. we investigated genetic variation in east african cassava mosaic cassava cameroon virus (eacmcv) from naturally infected cassava and from experimentally infected nicotiana benthamiana. results showed that the populations of eacmcv in cassava and in n. benthamiana were genetically heterogeneous. mu ... | 2011 | 21429548 |
| a common set of developmental mirnas are upregulated in nicotiana benthamiana by diverse begomoviruses. | begomoviruses are single-stranded dna viruses that cause economically important diseases of many crops throughout the world and induce symptoms in plants, including enations, leaf curling and stunting, that resemble developmental abnormalities. micrornas (mirnas) are small endogenous rnas that are involved in a variety of activities, including plant development, signal transduction and protein degradation, as well as response to environmental stress, and pathogen invasion. | 2011 | 21447165 |
| effects of various plant protein sources in high-quality feed block on feed intake, rumen fermentation, and microbial population in swamp buffalo. | this study was designed to determine effect of various plant protein sources in high-quality feed block (hqfb) on feed intake, rumen fermentation, and microbial population in swamp buffalo. four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly assigned according to a 4 × 4 latin square design. four kinds of plant protein sources (coarse rice bran (crb), cassava hay (ch), phaseolus calcaratus hay, and mulberry hay (mh)) were mixed in the hqfb. hqfbs were allowed to be licked at fr ... | 2011 | 21479843 |
| effect of cassava starch coating on quality and shelf life of fresh-cut pineapple (ananas comosus l. merril cv "pérola"). | this research studied the influence of treatment with ascorbic acid, citric acid, and calcium lactate dipping and cassava starch edible coatings on quality parameters and shelf life of fresh-cut pineapple in slices during 12 d at 5 °c. after previous tests, the treatments selected for this study were samples dipped into antibrowning solution with 0.5% of ascorbic acid and 1% of citric acid, with and without 2% of calcium lactate and coated with 2% of cassava starch suspensions. changes in weight ... | 2011 | 21535677 |
| comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in nicotiana benthamiana and their correlation with the levels of developmental mirnas. | abstract: background: whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. begomoviruses differ in the number of components that make up their genomes and association with satellites, and yet they cause strikingly similar phenotypes, such as leaf curling, chlorosis and stunted plant growth. micrornas (mirnas) are small endogenous rnas that regulate plant growth and development. the study described ... | 2011 | 21592402 |
| frozen chicken for wild fish: nutritional transition in the brazilian amazon region determined by carbon and nitrogen stable isotope ratios in fingernails. | objectives: amazonian populations are experiencing dietary changes characteristic of the nutrition transition. however, the degree of change appears to vary between urban and rural settings. to investigate this process, we determined carbon and nitrogen stable isotope ratios in fingernails and dietary intake of amazonian populations living along a rural to urban continuum along the solimões river in brazil. methods: carbon and nitrogen stable isotope ratios were analyzed from the fingernails of ... | 2011 | 21630371 |
| genetic characterization of cassava (manihot esculenta) landraces in brazil assessed with simple sequence repeats. | based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava (manihot esculenta) landraces from selected regions in brazil, and examine how this variety is distributed according to origin in several municipalities in the states of minas gerais, são paulo, mato grosso do sul, amazonas and mato grosso. high diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic lo ... | 2009 | 21637653 |
| uterine contraction induced by tanzanian plants used to induce abortion. | ethnopharmacological relevance: women in tanzania use plants to induce abortion. it is not known whether the plants have an effect. aims of study: collect data on plant use in relation to induced abortion and test the effect of plant extracts on uterine contraction. methods: during interviews with traditional birth attendants and nurses, plants were identified. cumulative doses of plant extracts were added to rat uterine tissue in an organ bath, and the force and frequency of contractions record ... | 2011 | 21645605 |
| [microbial oil production by trichosporon cutaneum b3 using cassava starch]. | microbial oil, as raw material for biodiesel, can be produced by trichosporon cutaneum b3 using cassava starch hydrolysate. batch cultures demonstrated that there was little inhibitory effect with the concentration of cassava starch hydrolysate up to 90 g/l. the favorable initial ph, c/n molar ratio, nitrogen source and its concentration were 6.0, 116, yeast extract and 3.0 g/l, respectively. under the optimized conditions, dry biomass reached 15.2 g/l and lipid content reached 40.9% after cultu ... | 2011 | 21650027 |
| effects of different starch sources on bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets. | the study was conducted to evaluate the effects of different starch sources on bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets. twenty-eight pic male piglets were divided into four homogeneous groups according to initial body weight (similar birth and parity, weaned at 21 ± 1.5 days). diets for the four treatments consisted of corn starch, wheat starch, tapioca starch and pea starch with the determined ratio for amylose to amylopec ... | 2011 | 21660473 |
| folate analysis in foods by uplc-ms/ms: development and validation of a novel, high throughput quantitative assay; folate levels determined in australian fortified breads. | an ultra-performance liquid chromatography-tandem mass spectrometry method was developed, optimised and validated for the quantification of synthetic folic acid (fa), also called pteroyl-l: -glutamic acid or vitamin b9 and naturally occurring 5-methyltetrahydrofolate (5-mthf) found in folate-fortified breads. optimised sample preparation prior to analysis involved addition of (13)c(5) labelled internal standards, treatments with +¦-amylase and rat serum, solid-phase extraction using aromatic-sel ... | 2011 | 21667347 |
| biotechnological approaches to determine the impact of viruses in the energy crop plant jatropha curcas. | abstract: background: geminiviruses infect a wide range of plant species including jatropha and cassava both belonging to family euphorbiaceae. cassava is traditionally an important food crop in sub - saharan countries, while jatropha is considered as valuable biofuel plant with great perspectives in the future. results: a total of 127 jatropha samples from ethiopia and kenya and 124 cassava samples from kenya were tested by enzyme-linked immunosorbent assay (elisa) for rna virus and polymerase ... | 2011 | 21812981 |
| influence of urea-calcium mixtures as rumen slow-release feed on in vitro fermentation using a gas production technique. | in this experiment the effects of different urea products (urea [u] and urea-calcium mixtures [ucm]) on rumen fermentation were investigated in dependence of different energy sources by using in vitro techniques. the 7 x 2 factorial arrangement followed a completely randomised design using seven urea products (u100, u40cacl2, u50cacl2, u60cacl2, u40caso4, u50caso4 and u60caso4) in combination with cassava chips (cc) or corn meal (cm). compared with other treatments, the cumulative gas production ... | 2011 | 21776840 |
| haematological and serum biochemical parameters of west african dwarf goats fed dried cassava leaves-based concentrate diets. | the study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of west african dwarf (wad) goats. the study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. at the start of the experiment, packed cell volume (pcv ... | 2011 | 21744028 |
| antifungal activity of 2 lactic acid bacteria of the weissella genus isolated from food. | ôçé in the present study, a total of 116 lactic acid bacteria (lab) strains isolated from mill flour and fermented cassava were screened for their antifungal activity. three strains among 116 were selected for their strongest inhibitory activity against food molds. these 3 strains were lactobacillus plantarum ve56, weissella cibaria fmf4b16, and w. paramesenteroides lc11. the compounds responsible for the antifungal activity were investigated. the strains displayed an inhibitory activity against ... | 2011 | 21729073 |
| characterization and expression profile of two udp-glucosyltransferases, ugt85k4 and ugt85k5, catalyzing the last step in cyanogenic glucoside biosynthesis in cassava. | manihot esculenta (cassava) contains two cyanogenic glucosides, linamarin and lotaustralin, biosynthesized from l-valine and l-isoleucine, respectively. in this study, cdnas encoding two uridine diphosphate glycosyltransferase (ugt) paralogs, assigned the names ugt85k4 and ugt85k5, have been isolated from cassava. the paralogs display 96% amino acid identity, and belong to a family containing cyanogenic glucoside-specific ugts from sorghum bicolor and prunus dulcis. recombinant ugt85k4 and ugt85 ... | 2011 | 21736650 |
| enzymatic degradation of granular potato starch by microbacterium aurum strain b8.a. | microbacterium aurum strain b8.a was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. extracellular enzymes hydrolyzing granular starch were detected in the growth medium of m. aurum b8.a, while the type strain m. aurum dsmz 8600 produced very little amylase activity, and hence was unable to degrade granular starch. the strain b8.a extracellular enzyme fraction degraded wheat, tapioca and potato starch ... | 2011 | 21732245 |
| potential fungal inhibition by immobilized hydrolytic enzymes from trichoderma asperellum. | the use of cell wall degrading enzymes from trichoderma asperellum immobilized on biodegradable support is an alternative for food packaging. in this study, hydrolytic enzymes produced by t. asperellum were tested as a fungal growth inhibitor, in free form or immobilized on a biodegradable film composed of cassava starch and poly(butylene adipate-co-terephtalate) (pbat). the inhibitory activity was tested against aspergillus niger , penicillium sp., and sclerotinia sclerotiorum , microorganisms ... | 2011 | 21726085 |
| biofortification: a new tool to reduce micronutrient malnutrition. | the density of minerals and vitamins in food staples eaten widely by the poor may be increased either through conventional plant breeding or through the use of transgenic techniques, a process known as biofortification. | 2011 | 21717916 |
| simultaneous saccharification and continuous fermentation of sludge-containing mash for bioethanol production by saccharomyces cerevisiae chfy0321. | a continuous process was employed to improve the volumetric productivity of bioethanol production from cassava mash containing sludge and to simplify the process of ethanol production from cassava. after raw cassava powder was liquefied, it was used directly in a continuous process without sludge filtration or saccharification. a fermentor consisting of four linked stirrer tanks was used for simultaneous saccharification and continuous fermentation (sscf). although the mash contained sludge, con ... | 2011 | 21723335 |
| effects of propionic acid and ph on ethanol fermentation by saccharomyces cerevisiae in cassava mash. | the effects of propionic acid on ethanol and glycerol production by saccharomyces cerevisiae in cassava mash were examined along with the influence of ph (4.0, 5.0, and 6.0) and of dissolved solids content (22%, 25%, and 27%). inhibition by propionic acid increased as solids content increased and medium ph declined. complete inhibition of ethanol fermentation was observed in mashes at ph-á4.0 (60-ámm propionic acid for 22% solids and 45-ámm for 25% and 27%). glycerol production linearly decrease ... | 2011 | 21701869 |
| a high-amylopectin diet caused hepatic steatosis associated with more lipogenic enzymes and increased serum insulin concentration. | starch is the major energy source for monogastric mammals and humans. the present study was conducted to evaluate the liver metabolic responses of weaned pigs fed with different dietary starches. a total of sixteen weaned pigs were fed with two experimental diets containing either cassava starch (cs, 80 % amylopectin and 20 % amylose) or maize starch (70 % amylopectin and 30 % amylose). the present results showed that the growth performance was not affected by different dietary starches (p>0·05) ... | 2011 | 21736804 |
| adequate wound care and use of bed nets as protective factors against buruli ulcer: results from a case control study in cameroon. | buruli ulcer is an infectious disease involving the skin, caused by mycobacterium ulcerans. its exact transmission mechanism remains unknown. several arguments indicate a possible role for insects in its transmission. a previous case-control study in the nyong valley region in central cameroon showed an unexpected association between bed net use and protection against buruli ulcer. we investigated whether this association persisted in a newly discovered endemic buruli ulcer focus in bankim, nort ... | 2011 | 22087346 |
| Anaerobic biological treatment of high strength cassava starch wastewater in a new type up-flow multistage anaerobic reactor. | Anaerobic treatment of cassava starch wastewater using an up-flow multistage anaerobic reactor was investigated. The results showed that startup was successfully accomplished in 22d. The maximum 87.9% chemical oxygen demand (COD) was removed at hydraulic retention time (HRT) of 6.0h at fixed concentration 4000mg/L. In addition, 77.5-92.0% COD were removed as organic loading rates at 10.2-40.0kgCOD/(m(3)d) at fixed HRT of 6.0h. The Grau second-order kinetic model and modified Stover-Kincannon mod ... | 2012 | 22169215 |
| genome sequence of lactobacillus rhamnosus strain casl, an efficient l-lactic acid producer from cheap substrate cassava. | lactobacillus rhamnosus is a type of probiotic bacteria with industrial potential for l-lactic acid production. we announce the draft genome sequence of l. rhamnosus casl (2,855,156 bp with a g+c content of 46.6%), which is an efficient producer of l-lactic acid from cheap, nonfood substrate cassava with a high production titer. | 2011 | 22123765 |
| Low genetic diversity of Squash vein yellowing virus in wild and cultivated cucurbits in the U.S. suggests a recent introduction. | Squash vein yellowing virus (SqVYV) isolates were collected from cultivated and weedy cucurbits representing major hosts and locations in the U.S. and analyzed to better understand the diversity and population structure. No differences in symptoms were observed in field-collected isolate source plants or subsequently inoculated greenhouse plants, and the complete genome of an SqVYV isolate from a wild cucurbit host (smellmelon, Cucumis melo var. dudaim) was highly similar (99.4% nucleotide ident ... | 2011 | 22142477 |
| Butanol and ethanol production from tapioca starch wastewater by Clostridium spp. | Total (TWW) and tapioca starch wash wastewater (TSWW) from a cassava processing plant in Thailand were analyzed for their composition with a view to evaluate their potential as substrates for solvent production by ABE fermentation with Clostridium spp. Starch was detected at a 1.63-fold higher level in the TWW than that in the TSWW (24.4% and 15.0% (w/w), respectively). The chemical oxygen demand (COD) was broadly similar (20,093 and 20,433 mg/L), but the biological oxygen demand (BOD) was 1.84- ... | 2011 | 22020468 |
| household utilization of manioc (manihot esculenta crantz) in northern mozambique. | mozambique is ranked ninth of top manioc (manihot esculenta crantz) producing countries in the world. manioc roots are a staple to people living in the northern part of the country. despite this, information on production, utilization, postharvest handling, and marketing is scarce. the purpose of this baseline study was to document selected information on manioc, from the production to marketing stage. to gather this information, 70 focus groups consisting of 1,724 participants purposely sampled ... | 2010 | 21888575 |
| cryptococcus thermophilus sp. nov., isolated from cassava sourdough. | a novel anamorphic yeast, strain lth 6662t, was isolated from cassava sourdough. the isolate supposedly originated from cassava flour or was a contaminant thereof. sequencing of the d1/d2 domain of the 26s rrna gene indicated that strain lth 6662t represents a new species. its closest relatives were the species from the cryptococcus humicola complex. the novel strain had several physiological characteristics that differed from those of related species: the ability to assimilate raffinose and cad ... | 2011 | 22021579 |
| Quantification of total iodine in intact granular starches of different botanical origin exposed to iodine vapor at various water activities. | Iodine has been used as an effective tool for studying both the structure and composition of dispersed starch and starch granules. In addition to being employed to assess relative amylose contents for starch samples, it has been used to look at the molecular mobility of the glucose polymers within intact starch granules based on exposure to iodine vapor equilibrated at different water activities. Starches of different botanical origin including corn, high amylose corn, waxy corn, potato, waxy po ... | 2011 | 21962455 |
| multiplex rt-pcr assays for the simultaneous detection of both rna and dna viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in east africa. | uniplex and multiplex reverse transcription-polymerase chain reaction (rt-pcr) protocols were developed for the detection of cassava brown streak viruses (cbsvs) in single and mixed infections with cassava mosaic begomoviruses (cmbs) in a tropical crop plant, cassava (manihot esculenta). cmbs contain ssdna as their genome (genus begomovirus, family geminiviridae) while cbsvs are made up of positive sense ssrna (genus ipomovirus, family potyviridae), and they cause the economically important cass ... | 2012 | 22080852 |
| automatic segmentation on multiple starch granules from microscopic images. | starch is the most important carbohydrate in the human diet and contained in many staple foods such as rice, wheat, corn, potatoes and cassava, etc. currently, microscopic imaging technology is one of the most effective ways to investigate and analyze the structure of starches. segmentation of starch granules is a necessary step for starch granule structure analysis such as starch granules detection, shape recognition, and size computation. this article investigates a new method based on gvf (gr ... | 2011 | 21997964 |
| influence of processing cassava peels on the hydrogen cyanide concentration, nutritive value and performance of growing rabbits. | forty-eight rabbits used to investigate the response of growing rabbits to diets containing differently processed cassava peel meals were divided into four groups balanced for sex and weight. these groups were then assigned at random to the experimental treatments. four diets were formulated such that the diets contained 200 g/kg of the experimental feedstuffs, sun-dried cassava peel meal (s), ensiled cassava peel meal (e) and retted cassava peel meal (r), and the control did not contain any cas ... | 2012 | 22086411 |
| molecular interaction between two cassava geminiviruses exhibiting cross-protection. | there are increasing reports of geminivirus mixed infections of field plant hosts. these mixed infections have been suggested to result in recombinations, emergence of new viruses and new disease epidemics. we previously reported the occurrence of mixed infection between african cassava mosaic virus (acmv) and east african cassava mosaic cameroon virus (eacmcv) resulting in severe symptoms in cassava fields in cameroon. here, we show that reassortment of dna-a and dna-b components of acmv and ea ... | 2011 | 21925553 |
| Effect of carbohydrate sources and cotton seed meal in the concentrate: II. Feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in beef cattle. | Four, rumen fistulated crossbred (Brahman × native) beef cattle steers were randomly assigned to receive four dietary treatments according to a 2?×?2 factorial arrangement in a 4?×?4 Latin square design. Factor A was carbohydrate source; cassava chip (CC) or CC + rice bran at a ratio 3:1 (CR3:1) and Factor B was cotton seed meal level (CM); and 109 g CP/kg (LCM) and 328 g CP/kg (HCM) at similar overall CP levels (490 g CP/kg). The animals were fed 5 g concentrate/kg BW, and urea-treated rice str ... | 2012 | 22072076 |
| top 10 plant viruses in molecular plant pathology. | many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. however, to our knowledge, no such list exists. the aim of this review was to survey all plant virologists with an association with molecular plant pathology and ask them to nominate which plant viruses they would place in a 'top 10' based on scientific/economic importance. the survey generated more than 250 votes from the international community, and allowed the gen ... | 2011 | 22017770 |
| influence of retinoblastoma-related gene silencing on the initiation of dna replication by african cassava mosaic virus rep in cells of mature leaves in nicotiana benthamiana plants. | abstract: background: geminiviruses mainly infect terminally differentiated tissues and cells in plants. they need to re-programme host cellular machinery for dna replication. this process is thought to be mediated by inactivation of cell-cycle repressor proteins and by induction of host dna synthesis protein expression through actions of the geminviral replication initiator protein (rep). findings: exploiting a nicotiana benthamiana pori2 line, which is transformed with a transgene consisting ... | 2011 | 22204717 |
| effect of dietary vitamin e supplementation on pork quality. | the purpose of this experiment was to examine the effect of dietary vitamin e supplementation on pork quality, and in particular on colour stability. crossbred pigs (n = 72) at a mean weight of 44 kg were assigned to one of two treatments. one group received, during a period of 84 days prior to slaughter, a tapioca based diet, which contained 8 mg vitamin e per kg feed. the other group received during this period the same diet, except it was supplemented with 200 mg vitamin e per kg feed. muscle ... | 1998 | 22063309 |
| prebiotic effects of cassava fibre as an ingredient in cracker-like products. | cassava fibre, a waste product formed in starch production, was incorporated into wheat to give composite flours at ratios of 80/20, 70/30, 60/40 and 50/50, respectively. a cracker-like biscuit was produced from the preferred ratios (60/40 and 50/50). the effects of these on diets as a prebiotic were evaluated in a rat assay. determinations of the proximate composition and haematological parameters were made as well as microbiological analysis. the protein content of the cracker-like product bas ... | 2011 | 22105619 |
| A tribute to Dr. Frank I Tovey on his 90th birthday. | This paper pays a tribute to Dr. Frank I Tovey on his 90th birthday which happens on September 1, 2011, and briefly describes the major findings in his research career and contributions as follows. The geographical prevalence of duodenal ulceration is related to staple diets. Unrefined wheat and maize, soya, certain pulses and millets are associated with a low prevalence while refined wheat, maize and rice, yams, cassava and green banana with a high prevalence. Predominant foodstuffs from low pr ... | 2011 | 21987600 |
| Conversion of Sterically Demanding a,a-Disubstituted Phenylacetonitriles by the Arylacetonitrilase from Pseudomonas fluorescens EBC191. | The nitrilase from Pseudomonas fluorescens EBC191 converted 2-methyl-2-phenylpropionitrile, which contains a quaternary carbon atom in the a-position toward the nitrile group, and also similar sterically demanding substrates, such as 2-hydroxy-2-phenylpropionitrile (acetophenone cyanohydrin) or 2-acetyloxy-2-methylphenylacetonitrile. 2-Methyl-2-phenylpropionitrile was hydrolyzed to almost stoichiometric amounts of the corresponding acid. Acetophenone cyanohydrin was transformed to the correspond ... | 2012 | 22020513 |
| Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour. | Cassava is a starch-containing root crop that is widely used as a raw material in a variety of industrial applications, most recently in the production of fuel ethanol. In the present study, ethanol production from raw (uncooked) cassava flour by simultaneous saccharification and fermentation (SSF) using a preparation consisting of multiple enzyme activities from Aspergillus kawachii FS005 was investigated. The multi-activity preparation was obtained from a novel submerged fermentation broth of ... | 2011 | 22072435 |
| a consolidated bio-processing of ethanol from cassava pulp accompanied by hydrogen production. | a biphasic fermentation approach was undertaken for the production of ethanol and hydrogen from cassava pulp. the glucose generated by co-culture of clostridium thermocellum and thermoanaerobacterium aotearoense was 13.65±0.45 g l(-1), which was 1.75 and 1.17-fold greater than that produced by mono-cultures of c. thermocellum and t. aotearoense, respectively. the accumulated glucose could be utilised rapidly by subsequently inoculated saccharomyces cerevisiae. an inoculum ratio of 1:1, a thermop ... | 2011 | 21962537 |
| Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping. | Concentrated cassava bagasse hydrolysate (CBH) containing 584.4g/L glucose was studied for acetone-butanol-ethanol (ABE) fermentation with a hyper-butanol-producing Clostridiumacetobutylicum strain in a fibrous bed bioreactor with gas stripping for continuous butanol recovery. With periodical nutrient supplementation, stable production of n-butanol from glucose in the CBH was maintained in the fed-batch fermentation over 263h with an average sugar consumption rate of 1.28g/Lh and butanol product ... | 2012 | 22101071 |
| Pig performance increases with the addition of DL-methionine and L-lysine to ensiled cassava leaf protein diets. | Two studies were conducted to determine the impact of supplementation of diets containing ensiled cassava leaves as the main protein source with synthetic amino acids, DL-methionine alone or with L-lysine. In study 1, a total of 40 pigs in five units, all cross-breds between Large White and Mong Cai, with an average initial body weight of 20.5 kg were randomly assigned to four treatments consisting of a basal diet containing 45% of dry matter (DM) from ensiled cassava leaves (ECL) and ensiled ca ... | 2012 | 21947959 |
| Effect of adaptation strategies when feeding fresh cassava foliage on intake and physiological responses of lambs. | The objective of the experiment was to study different adaptation strategies to avoid HCN intoxication when feeding fresh cassava foliage to sheep. Twenty-four Phan Rang lambs (initial weight?=?19.6 kg at 5.5 months of age) were used in the study. The four experimental diets contained guinea grass (Panicum maximum) supplemented with concentrate at 1.5% of body weight (BW) as dry matter (DM) (control) or supplemented with fresh cassava foliage (FCF) that was introduced into the diet with an adapt ... | 2012 | 22081316 |
| The effect of sulfide and ammonia on cassava fermentation for ethanol production in an ethanol-methane coupled system. | An ethanol-methane coupled system was proposed to resolve wastewater pollution in cassava ethanol production. The wastewater originated from ethanol distillation is treated with two-stage anaerobic digestion and then recycled for medium preparation for the next batch ethanol fermentation, thus eliminating wastewater discharge and saving fresh water. The constituents of the two-stage anaerobic digestion effluent were complex which influenced the ethanol fermentation performance. This paper aimed ... | 2011 | 22105130 |
| protein improvement in gari by the use of pure cultures of microorganisms involved in the natural fermentation process. | the ability of microorganisms involved in cassava mash fermentation to produce and improve protein value by these microorganisms during fermentation was studied. standard microbiological procedures were used to isolate, identify and determine the numbers of the organisms. alcaligenes faecalis, lactobacillus plantarum, bacillus subtilis, leuconostoc cremoris, aspergillus niger, a. tamari, geotrichum candidum and penicillium expansum were isolated and identified from cassava waste water while stan ... | 2011 | 22514894 |
| study of the effects of hydrogen cyanide exposure in cassava workers. | hydrogen cyanide is the chemical responsible for tissue hypoxia. chronic exposure to hcn may cause neurological, respiratory, cardiovascular and thyroid defects. onset of symptoms depends on dose and duration of exposure. large scale of cassava processing could be disastrous due to discharge of hydrocyanic acid into the air. cassava processing is the major industrial work in and around salem. hence the present study is taken to assess the effects of hcn exposure in cassava workers. | 2011 | 22412292 |
| a geminivirus-satellite complex is associated with leaf deformity of mentha (mint) plants in punjab. | a widespread leaf deformity disease of mentha (mint), accompanied by whiteflies, the vectors of begomoviruses, was observed in punjab in the last few years. the presence of begomovirus was indicated by dna dot-blot analysis using the conserved coat protein and replication-associated protein genes of another begomovirus, sri lankan cassava mosaic virus (slcmv). a dna fragment (2.0 kb), representing a partial genomic dna of a begomovirus, amplified from the symptomatic mentha leaves was used to de ... | 2011 | 23637488 |
| potential of using multiscale kenaf fibers as reinforcing filler in cassava starch-kenaf biocomposites. | biodegradable materials made from cassava starch and kenaf fibers were prepared using a solution casting method. kenaf fibers were treated with naoh, bleached with sodium chlorite and acetic buffer solution, and subsequently acid hydrolyzed to obtain cellulose nanocrystals (cncs). biocomposites in the form of films were prepared by mixing starch and glycerol/sorbitol with various filler compositions (0-10 wt%). x-ray diffraction revealed that fiber crystallinity increased after each stage of tre ... | 2012 | 23399291 |
| prediction of gross energy and digestible energy in copra meal, palm kernel meal, and cassava root fed to pigs. | many of the available prediction equations for feed energy value may not be applicable for ingredients such as copra (cocos nucifera) meal (cm), palm kernel meal (pkm), and cassava (manihot esculenta) root (cr). therefore, we developed novel equations for estimating ge and de concentrations in cm, pkm, cr, and diets containing these ingredients. data for ge and de concentrations were obtained from previous experiments in which the chemical composition in the ingredients and diets were determined ... | 2012 | 23365336 |
| digestible and metabolizable energy concentrations in copra meal, palm kernel meal, and cassava root fed to growing pigs. | an experiment was conducted to measure de and me in copra (cocos nucifera) meal (cm), palm kernel meal (pkm), and cassava (manihot esculenta) root (cr) in growing pigs. eight boars with an initial bw of 67.3 ± 5.8 kg were individually housed in metabolism crates that were equipped with a feeder and a nipple drinker. a replicated 4 × 4 latin square design was used with 4 dietary treatments, 4 periods, and 8 animals. a basal diet mainly contained corn (zea mays) and soybean (glycine max) meal. thr ... | 2012 | 23365309 |
| quality evaluation of stiff porridges prepared from irish potato (solanum tuberosum) and pigeon pea (cajanus cajan) starch blends. | quality attributes of stiff porridges prepared from irish potato and pigeon pea starch blends were studied. starches were extracted from irish potato and pigeon pea using a wet extraction method. various ratios of the starches were mixed and analyzed for chemical, functional and pasting properties. the starch blends were then prepared into stiff porridges for sensory evaluation using a 20-man sensory panel. substitution of irish potato starch with pigeon pea starch led to increases in protein (0 ... | 2011 | 23729855 |
| genetic diversity analysis of cyanogenic potential (cnp) of root among improved genotypes of cassava using simple sequence repeat markers. | cyanogenic potential (cnp) of cassava constitutes a serious problem for over 500 million people who rely on the crop as their main source of calories. genetic diversity is a key to successful crop improvement for breeding new improved variability for target traits. forty-three improved genotypes of cassava developed by international institute of tropical agriculture (ita), ibadan, were characterized for cnp trait using 35 simple sequence.repeat (ssr) markers. essential colorimetry picric test wa ... | 2012 | 23678653 |
| [determination of deoxynivalenol in grain and its products by solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry]. | a method was established for the determination of deoxynivalenol (vomitoxin) in grain and its products based on solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms). the sample was firstly extracted by acetonitrile-water (84:16, v/v). the extract was then cleaned-up by an hlb solid phase extraction cartridge. the separation was carried out on a phenomenex kinetex c18 column (100 mm x4. 6 mm, 2.6 microm) with a gradient elution using 0.3 ... | 2012 | 23451526 |
| the nigeria high glycemic index starchy foods, obesity, and the environment. | glycemic index has generated a lot of debate for nearly 30 years, especially as it relates to the control for the treatment of diabetes. | 2011 | 23175893 |
| comparative continuous-indirect-calorimetry study of two carbohydrates with different glycemic indices. | six healthy young men were studied by indirect calorimetry for 6 h after eating a meal composed of glucose or manioc starch (equivalent to 50 g dextrose). blood was drawn every 30 min for 6 h to measure plasma glucose, free fatty acid (ffa), and insulin concentrations. the glycemic index of the starch was 57%. plasma insulin and glucose concentrations were significantly higher from 150 to 210 min and ffa concentrations remained significantly lower from 210 to 360 min after starch than after gluc ... | 2007 | 1951156 |
| temperature effects on the binding efficiency of starch pastes. | the effect of temperature on the binding efficiency of yam starch and cassava starch pastes has been investigated. starch pastes prepared at different temperatures were used to granulate lactose powder and the resulting granules and tablet properties were evaluated. increased average granule size and decreased percentage fines were observed as the pasting temperature was increased upto a certain level and then there was a decrease in average size and increase in amount of fines as the pasting te ... | 2014 | 4023034 |
| effects of tapioca obtained from cassava (manihot utilissima) on the disintegration and dissolution rates of paracetamol tablets. | the properties of tapioca obtained from cassava (manihot utilissima) have been evaluated. its binding effect in tablets of paracetamol on the disintegration and dissolution rates was compared with tablets prepared with polyvinylpyrrolidone and gelatin. the nature and amount of the binders were found to alter the disintegration and dissolution rates of the tablets by reducing their wettability as measured by the adhesion tension of water. a linear relationship has been found to exist between the ... | 2008 | 2565972 |
| more lingual surface attrition of the maxillary anterior teeth in american indians: prehistoric panamanians. | lingual surface attrition of the maxillary anterior teeth (lsa-mat) has been found in additional prehistoric latin american indian skeletons. lsamat was first observed in crania from an archaic brazilian site. this second finding occurs in teeth from venado beach, a late prehistoric site in panama. lsamat is also present in some fragmentary specimens from the archaic cerro mangote site in panama. lsamat at venado beach is present in 57% of 28 adult crania. as in the brazilian study, lsamat is as ... | 2012 | 3303956 |
| [alcohol from mandioca by continuous fermentation]. | | 2015 | 4524330 |
| acute cassava poisoning in children in sarawak. | | 1989 | 209589 |
| association of hyperglycemia with dietary cyanogen and socio-economic level. the study of rural communities in south-east nigeria. | an association of asymptomatic hyperglycemia with dietary cyanogens and socio-economic level was ascertained in 2,000 volunteers in rural communities in south-east nigeria. the staples of the poor consisted of one or two bulky carbohydrate meals (derivatives of different species of cocoyam, cassava, yam and maize) eaten with vegetable soup in palm oil, melon seeds, snail, occasional meat and fish. the diet of the high income class included also beans, rice and more animal proteins. alcohol (main ... | 1981 | 1862689 |
| an enzymatic assay for the total cyanide content of cassava (manihot esculenta crantz). | | 1999 | 96301 |
| cyanide content of cassava mash and gari flour and influence of water activity (aw) during storage. | | 2009 | 2713530 |
| diet, pancreatic function, and chronic pancreatitis in south india and france. | the usual consumption of calories, fat, protein, and carbohydrate, and the exocrine pancreatic function estimated in duodenal juice after an intravenous injection of secretin and cholecystokinin (cck), have been studied with the same method and by the same team in kerala (south india) and in marseille (france) in apparently normal children (7 indians, 21 french), in normal adults (23 indians, 17 french), and in patients presenting with chronic calcifying pancreatitis (8 indian children, 28 india ... | 2012 | 3362842 |
| anti-sickling effect of dietary thiocyanate in prophylactic control of sickle cell anemia. | as a clinical entity, sickle cell anemia (sca) is known to be relatively rarer in africans than in the african-american population of the united states. paradoxically, sickle cell trait (sct), the non-anemic, heterozygous condition, is about three times more common among indigenous africans than in african-americans. the ratio of sca to sct is 1:50 for african-americans, and less than 1:1,000 for tropical africans. this etiological disparity is attributed to an anti-sickling agent, thiocyanate, ... | 2013 | 3795284 |
| biochemical studies in tanzanian patients with ataxic tropical neuropathy. | data on thiocyanate and vitamin b(12) concentrations in plasma from tanzanian patients with ataxic tropical neuropathy are presented and support the hypothesis that, as in nigeria, the condition may result from chronic exposure to cyanide or cyanogens from a diet including large amounts of cassava. | 2015 | 5026008 |
| cassava - a valuable food but a possible poison. | | 1989 | 209588 |
| [insulin secretion in nondiabetic manioc consumers]. | the study of pancreatic beta cell function during an intravenous glucose tolerance test performed on 28 healthy cassava eaters showed 3 types of response: normal insulin secretion in 17 subjects, a significantly elevated peak at 30 min, compared to the control group (6 subjects p less than 0.0125), with preservation of insulin secretion dynamics, and a late peak at 120 min. (5 subjects p less than 0.0125). among the different factors capable of stimulating insulin secretion, in absence of a sign ... | 1982 | 1915997 |
| cranioschisis aperta with encephaloschisis in cephalothoracopagus hamster twins. | the results of gross and histopathological study of a near-term male hamster exencephalic lateral cephalothoracopagus are presented. there was minimal duplication of the internal organs to the point of division at the abdomen. the appendicular skeleton was relatively unaffected by the severe malformations of the axial skeleton. the studies suggested that the lateral relationship of the skull to the spinal columns was a consequence of the presence of two embryonic neural tubes; the chordomesoderm ... | 2013 | 4016585 |
| the effects of interacting variables on the tensile strength, disintegration and dissolution of paracetamol tablets. | a factorially designed scheme has been used to analyse the separate and combined effects of packing fraction (p), nature of binder (n) and concentration of binder (c) on the tensile strength, disintegration and dissolution (t50%) times of paracetamol tablets. in general, p has the greatest effect on tensile strength, disintegration and dissolution times followed by c then n. for the variables in combination, the ranking of the effects on tensile strength, for the pvp/gelatin formulations, are p ... | 2010 | 2900309 |
| cyanide, cassava, and diabetes. | | 2001 | 90295 |
| letter: manioc unsuitable for turkeys. | | 2016 | 4770601 |
| effects of cassava variety and processing on energy and protein digestibility and utilization by young children. | two varieties of cassava, processed as nigerian fermented flour (gari) or as brazilian flour (farinha), were fed to two groups of eight infants and young children, each group receiving both forms of one variety, with preceding, intervening and following casein control diets. the flours provided 50% of diet energy, with casein added to make 8% energy as protein, vegetable oils to make 20% as fat and corn syrup solids and sugar to make 72% carbohydrate (cho) energy. fecal wet weight increased (p l ... | 2012 | 3392598 |
| molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at são paulo state. | fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. ability to fix nitrogen was verified by the acetylene reduction assay. all potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifh probe derived from azospirillum brasilense. the strains were characterized by rapd, ardra and 16s rdna sequence analysis. rapd analyse ... | 2008 | 24031239 |
| linamarase expression in cassava cultivars with roots of low- and high-cyanide content. | this paper reports the expression and localization of linamarase in roots of two cassava (manihot esculenta crantz) cultivars of low and high cyanide. two different patterns of linamarase activity were observed. in the low-cyanide type, young leaves displayed very high enzyme activity during the early plant growing stage (3 months), whereas in root peel, the activity increased progressively to reach a peak in 11-month-old plants. conversely, in the high-cyanide cultivar (hcv), root peel linamara ... | 2002 | 12177481 |
| the comparatively proteomic analysis in response to cold stress in cassava plantlets. | cassava (manihot esculenta crantz) is a tropical root crop and sensitive to low temperature. however, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. an investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was carried out with the approaches of physiology and proteomics in the present study. the principal component analysis of seven physiological characteristics showed that electrolyte leakage (el), chlorophy ... | 2016 | 27881899 |