effect of waterlogging on carbohydrate metabolism and the quality of fiber in cotton (gossypium hirsutum l.). | transient waterlogging occurs frequently in the yangtze river and adversely affects cotton fiber quality. however, the carbohydrate metabolic mechanism that affects fiber quality after waterlogging remains undescribed. here, the effects of five waterlogging levels (0, 3, 6, 9, and 12 days) were assessed during flowering and boll formation to characterize the carbohydrates, enzymes and genes that affect the fiber quality of cotton after waterlogging. the cellulose and sucrose contents of cotton f ... | 2016 | 27446110 |
stability of spatial distributions of stink bugs, boll injury, and ndvi in cotton. | a 3-yr study was conducted to determine the degree of aggregation of stink bugs and boll injury in cotton, gossypium hirsutum l., and their spatial association with a multispectral vegetation index (normalized difference vegetation index [ndvi]). using the spatial analysis by distance indices analyses, stink bugs were less frequently aggregated (17% for adults and 4% for nymphs) than boll injury (36%). ndvi values were also significantly aggregated within fields in 19 of 48 analyses (40%), with ... | 2016 | 27436843 |
overexpression of ghsarp1 encoding a e3 ligase from cotton reduce the tolerance to salt in transgenic arabidopsis. | ubiquitination plays a very important role in the response to abiotic stresses of plant. to identify key regulators of salt stress, a gene ghsarp1(salt-associated ring finger protein)encoding c3h2c3-type e3 ligase, was cloned from cotton. transcription level of ghsarp1 was high in leaf, flower and fiber of 24,27 and 27dpa (days post-anthesis), but low in root and stem. except peg6000 treatment, the expression of ghsarp1 was down-regulated by nacl, cold and aba after being treated for 1 h. ghsarp ... | 2016 | 27402266 |
mining and analysis of snp in response to salinity stress in upland cotton (gossypium hirsutum l.). | salinity stress is a major abiotic factor that affects crop output, and as a pioneer crop in saline and alkaline land, salt tolerance study of cotton is particularly important. in our experiment, four salt-tolerance varieties with different salt tolerance indexes including cri35 (65.04%), kanghuanwei164 (56.19%), zhong9807 (55.20%) and cri44 (50.50%), as well as four salt-sensitive cotton varieties including hengmian3 (48.21%), gk50 (40.20%), xinyan96-48 (34.90%), zhongs9612 (24.80%) were used a ... | 2016 | 27355327 |
the cotton mitogen-activated protein kinase kinase 3 functions in drought tolerance by regulating stomatal responses and root growth. | mitogen-activated protein kinase (mapk) cascades play critical roles in signal transduction processes in eukaryotes. the mapk kinases (mapkks) that link mapkk kinases (mapkkks) and mapks are key components of mapk cascades. however, the intricate regulatory mechanisms that control mapkks under drought stress conditions are not fully understood, especially in cotton (gossypium hirsutum) here, we isolated and characterized the cotton group b mapkk gene ghmkk3 overexpressing ghmkk3 in nicotiana ben ... | 2016 | 27335349 |
optimal cotton insecticide application termination timing: a meta-analysis. | the concept of insecticide termination timing is generally accepted among cotton (gossypium hirsutum) researchers; however, exact timings are often disputed. specifically, there is uncertainty regarding the last economic insecticide application to control fruit-feeding pests including tarnished plant bug (lygus lineolaris (palisot de beauvois)), boll weevil (anthonomus grandis), bollworm (helicoverpa zea), tobacco budworm (heliothis virescens), and cotton fleahopper (pseudatomoscelis seriatus). ... | 2016 | 27329633 |
ssr-based association mapping of salt tolerance in cotton (gossypium hirsutum l.). | the identification of simple sequence repeat (ssr) markers associated with salt tolerance in cotton contributes to molecular assisted selection (mas), which can improve the efficiency of traditional breeding. in this study, 134 samples of upland cotton cultivars were selected. the seedling emergence rates were tested under 0.3% nacl stress. a total of 74 ssr markers were used to scan the genomes of these samples. to identify ssr markers associated with salt tolerance, an association analysis was ... | 2016 | 27323090 |
trichomes control flower bud shape by linking together young petals. | trichomes are widespread in plants and develop from surface cells on different tissues(1). they have many forms and functions, from defensive spines to physical barriers that trap layers of air to insulate against desiccation, but there is growing evidence that trichomes can also have developmental roles in regulating flower structure(2,3). we report here that the trichomes on petals of cotton, gossypium hirsutum l., are essential for correct flower bud shape through a mechanical entanglement of ... | 2016 | 27322517 |
cotton cytosolic pyruvate kinase ghpk6 participates in fast fiber elongation regulation in a ros-mediated manner. | cotton cytosolic pyruvate kinase ghpk6 is preferentially expressed in the late stage of fiber elongation process, transgenic experiments indicated that its expression level was negatively correlated to cell expansion rate. pyruvate kinase (pk) plays vital regulatory roles in rapid cell growth in mammals. however, the function of pk in plant cell growth remains unclear. in allotetraploid upland cotton (gossypium hirsutum l.), a total of 33 pk genes are encoded by the genome. analysis of the trans ... | 2016 | 27316434 |
leaf ontogeny strongly influences photosynthetic tolerance to drought and high temperature in gossypium hirsutum. | temperature and drought are major abiotic limitations to crop productivity worldwide. while abiotic stress physiology research has focused primarily on fully expanded leaves, no studies have investigated photosynthetic tolerance to concurrent drought and high temperature during leaf ontogeny. to address this, gossypium hirsutum plants were exposed to five irrigation treatments, and two different leaf stages were sampled on three dates during an abnormally dry summer. early in the growing season, ... | 2016 | 27302003 |
cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis. | cotton fibers arise through highly anisotropic expansion of a single seed epidermal cell. we obtained evidence that apical cell wall synthesis occurs through examining the tips of young elongating gossypium hirsutum (gh) and g. barbadense (gb) fibers. we characterized two tip types in gh fiber (hemisphere and tapered), each with distinct apical diameter, central vacuole location, and distribution of cell wall components. the apex of gh hemisphere tips was enriched in homogalacturonan epitopes, i ... | 2016 | 27301434 |
genome-wide analysis of long noncoding rnas and their responses to drought stress in cotton (gossypium hirsutum l.). | recent researches on long noncoding rnas (lncrnas) have expanded our horizon of gene regulation and the cellular complexity. however, the number, characteristics and expression patterns of lncrnas remain poorly characterized and how these lncrnas biogenesis are regulated in response to drought stress in cotton are still largely unclear. in the study, using a reproducibility-based rna-sequencing and bioinformatics strategy to analyze the lncrnas of 9 samples under three different environment stre ... | 2016 | 27294517 |
a cotton annexin affects fiber elongation and secondary cell wall biosynthesis associated with ca2+ influx, ros homeostasis, and actin filament reorganization. | annexins play pivotal roles in a variety of cellular processes as well as in fiber development; however, the functional mechanisms of their activities are unclear. here, an annexin gene that is preferentially expressed in fibers, ghfannxa, was found to be significantly associated with various cotton (gossypium hirsutum) fiber traits. transgenic analysis demonstrated that ghfannxa affected cotton fiber elongation and was involved in secondary cell wall (scw) biosynthesis. functional studies demon ... | 2016 | 27255486 |
effects of soil salinity on sucrose metabolism in cotton leaves. | this study investigated sucrose metabolism of the youngest fully expanded main-stem leaf (msl) and the subtending leaf of cotton (gossypium hirsutum l.) boll (lscb) of salt-tolerant (ccri-79) and salt-sensitive (simian 3) cultivars and its relationship to boll weight under low, medium and high soil salinity stress in dafeng, china, in 2013 and 2014. the results showed that with increased soil salinity, 1) both the chlorophyll content and net photosynthetic rate (pn) decreased, while the internal ... | 2016 | 27228029 |
genome-wide comparative analysis of the phospholipase d gene families among allotetraploid cotton and its diploid progenitors. | in this study, 40 phospholipase d (pld) genes were identified from allotetraploid cotton gossypium hirsutum, and 20 pld genes were examined in diploid cotton gossypium raimondii. combining with 19 previously identified gossypium arboreum pld genes, a comparative analysis was performed among the pld gene families among allotetraploid and two diploid cottons. based on the orthologous relationships, we found that almost each g. hirsutum pld had a corresponding homolog in the g. arboreum and g. raim ... | 2016 | 27213891 |
development and validation of a fluorescent multiplexed immunoassay for measurement of transgenic proteins in cotton (gossypium hirsutum). | in order to provide farmers with better and more customized alternatives to improve yields, combining multiple genetically modified (gm) traits into a single product (called stacked trait crops) is becoming prevalent. trait protein expression levels are used to characterize new gm products and establish exposure limits, two important components of safety assessment. developing a multiplexed immunoassay capable of measuring all trait proteins in the same sample allows for higher sample throughput ... | 2016 | 27177195 |
a genetic map between gossypium hirsutum and the brazilian endemic g. mustelinum and its application to qtl mapping. | among the seven tetraploid cotton species, little is known about transmission genetics and genome organization in gossypium mustelinum, the species most distant from the source of most cultivated cotton, g. hirsutum in this research, an f2 population was developed from an interspecific cross between g. hirsutum and g. mustelinum (hm). a genetic linkage map was constructed mainly using simple sequence repeat (ssrs) and restriction fragment length polymorphism (rflp) dna markers. the arrangements ... | 2016 | 27172208 |
the immature fiber mutant phenotype of cotton (gossypium hirsutum) is linked to a 22-bp frame-shift deletion in a mitochondria targeted pentatricopeptide repeat gene. | cotton seed trichomes are the most important source of natural fibers globally. the major fiber thickness properties influence the price of the raw material, and the quality of the finished product. the recessive immature fiber (im) gene reduces the degree of fiber cell wall thickening by a process that was previously shown to involve mitochondrial function in allotetraploid gossypium hirsutum here, we present the fine genetic mapping of the im locus, gene expression analysis of annotated protei ... | 2016 | 27172184 |
genome wide ssr high density genetic map construction from an interspecific cross of gossypium hirsutum × gossypium tomentosum. | a high density genetic map was constructed using f2 population derived from an interspecific cross of g. hirsutum × g. tomentosum. the map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cm of cotton genome with an average inter-marker distance of 1.48 cm. the maximum length of chromosome was 218.38 cm and the minimum was 122.09 cm with an average length of 167.90 cm. a sub-genome covers more genetic distance (2189.01 cm) with an average inter loci dist ... | 2016 | 27148280 |
epistasis together with partial dominance, over-dominance and qtl by environment interactions contribute to yield heterosis in upland cotton. | qtl mapping based on backcross and ril populations suggests that epistasis together with partial dominance, over-dominance and their environmental interactions of qtls play an important role in yield heterosis in upland cotton. a backcross population (bc) was constructed to explore the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). for yield and yield components, recombinant inbred line (ril) and bc populations were evaluated simultaneously at three different locations. a t ... | 2016 | 27138784 |
fine mapping and rna-seq unravels candidate genes for a major qtl controlling multiple fiber quality traits at the t1 region in upland cotton. | improving fiber quality is a major challenge in cotton breeding, since the molecular basis of fiber quality traits is poorly understood. fine mapping and candidate gene prediction of quantitative trait loci (qtl) controlling cotton fiber quality traits can help to elucidate the molecular basis of fiber quality. in our previous studies, one major qtl controlling multiple fiber quality traits was identified near the t1 locus on chromosome 6 in upland cotton. | 2016 | 27094760 |
fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (gossypium hirsutum). | high-quality cotton fibre equates to a more comfortable textile. fibre length is an important index of fibre quality. hydrogen peroxide (h2o2) acts as a signalling molecule in the regulation of fibre elongation. results from in vitro ovule culture suggest that the alteration of fibre cell h2o2 levels affects fibre development. ascorbate peroxidase (apx) is an important reactive oxygen species (ros) scavenging enzyme, and we found that ghapx1at/dt encoded one member of the previously unrealized g ... | 2016 | 27091877 |
genomic heterozygosity and hybrid breakdown in cotton (gossypium): different traits, different effects. | hybrid breakdown has been well documented in various species. relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. but correlations between genomic heterozygosity and vegetative and reproductive traits in cotton interspecific populations have not been studied. in the current study, two reciprocal f2 populations were developed using gossypium hirsutum cv. emian 22 and g. barbadense acc. 3-79 as parents to study hybri ... | 2016 | 27072350 |
construction of a high-density genetic map by specific locus amplified fragment sequencing (slaf-seq) and its application to quantitative trait loci (qtl) analysis for boll weight in upland cotton (gossypium hirsutum.). | upland cotton (gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for the industrial production and everyday use. next-generation sequencing is a powerful method to identify single nucleotide polymorphism markers on a large scale for the construction of a high-density genetic map for quantitative trait loci mapping. | 2016 | 27067834 |
comparative transmission genetics of introgressed chromatin in gossypium (cotton) polyploids. | introgression is widely acknowledged as a potential source of valuable genetic variation, and growing effort is being invested in analysis of interspecific crosses conferring transgressive variation. experimental backcross populations provide an opportunity to study transmission genetics following interspecific hybridization, identifying opportunities and constraints to introgressive crop improvement. the evolutionary consequences of introgression have been addressed at the theoretical level, ho ... | 2016 | 27056931 |
generation of transcriptome profiling and gene functional analysis in gossypium hirsutum upon verticillium dahliae infection. | verticillium wilt caused by the soil-borne fungus verticillium dahliae, is a devastating disease in cotton. to get more candidate genes related to wilt resistance, a normalized, full-length cdna library was used to generate the transcriptome profile from a wilt-resistant gossypium hirsutum variety (zhongzhimian kv3) upon v. dahliae strain v991 infection. total 3486 high-quality ests were focused from randomly selected 4000 clones, which included 3065 singletons and 421 contigs. to categorize the ... | 2016 | 27038549 |
insights into the ecology and evolution of polyploid plants through network analysis. | polyploidy is a widespread phenomenon throughout eukaryotes, with important ecological and evolutionary consequences. although genes operate as components of complex pathways and networks, polyploid changes in genes and gene expression have typically been evaluated as either individual genes or as a part of broad-scale analyses. network analysis has been fruitful in associating genomic and other 'omic'-based changes with phenotype for many systems. in polyploid species, network analysis has the ... | 2016 | 27027619 |
mapping of genes for flower-related traits and qtls for flowering time in an interspecific population of gossypium hirsutum × g. darwinii. | | 2016 | 27019451 |
genome-wide identification and analysis of the stress-resistance function of the tps (trehalose-6-phosphate synthase) gene family in cotton. | trehalose (a-d-glucopyranosyl a-d-glucopyranoside) is a nonreducing disaccharide and is widely distributed in bacteria, fungi, algae, plants and invertebrates. in the study, the identification of trehalose-6-phosphate synthase (tps) genes stress-related in cotton, and the genetic structure analysis and molecular evolution analysis of tpss were conducted with bioinformatics methods, which could lay a foundation for further research of tps functions in cotton. | 2016 | 26993467 |
simultaneous overexpression of the hherf2 and pedreb2a genes enhanced tolerances to salt and drought in transgenic cotton. | a desert-grown halimodendron halodendron ethylene-responsive element binding factor gene (hherf2), which encodes a 245 amino acids protein containing a conserved ap2/erebp domain, was isolated through the rapid amplification cdna end (race) method. sequence and phylogenetic analysis indicated that hherf2 was classified into the b-2 group of erf subfamily. semiquantitative rt-pcr showed that hherf2 was greatly induced by treatments with high-salt, drought and low temperature in h. halodendron see ... | 2016 | 26972971 |
critical roles of vacuolar invertase in floral organ development and male and female fertilities are revealed through characterization of ghvin1-rnai cotton plants. | seed number and quality are key traits determining plant fitness and crop yield and rely on combined competence in male and female fertilities. sucrose metabolism is central to reproductive success. it remains elusive, though, how individual sucrose metabolic enzymes may regulate the complex reproductive processes. here, by silencing vacuolar invertase (vin) genes in cotton (gossypium hirsutum) reproductive organs, we revealed diverse roles that vin plays in multiple reproductive processes. a se ... | 2016 | 26969720 |
structural analysis of gossypium hirsutum fibers grown under greenhouse and hydroponic conditions. | cotton is the one of the world's most important crops. like any other crop, cotton growth/development and fiber quality is highly dependent on environmental factors. increasing global weather instability has been negatively impacting its economy. cotton is a crop that exerts an intensive pressure over natural resources (land and water) and demands an overuse of pesticides. thus, the search for alternative cotton culture methods that are pesticide-free (biocotton) and enable customized standard f ... | 2016 | 26965558 |
data for proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.). | cotton is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to study the male sterile mechanisms of ccri9106, histological and itraq-facilitated proteomic analyses of anthers were performed. this data article contains data related to the research article titled itraq-facilitated proteomic profiling of ... | 2015 | 26958592 |
identification of stable quantitative trait loci (qtls) for fiber quality traits across multiple environments in gossypium hirsutum recombinant inbred line population. | the identification of quantitative trait loci (qtls) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (mas). in the present study, we used 28,861 simple sequence repeat (ssr) markers, which included 12,560 gossypium raimondii (d genome) sequence-based ssr markers to identify polymorphism between two upland cotton strains 0-153 and sgk9708. a total of 851 polymorphic primers were finally selected and used to genotype ... | 2016 | 26951621 |
anthocyanin accumulation enhanced in lc-transgenic cotton under light and increased resistance to bollworm. | breeding of naturally colored cotton fiber has been hampered by the limited germplasm, an alternative way is to use transgenic approach to create more germplasm for breeding. here, we report our effort to engineer anthocyanin production in cotton. the maize lc gene, under the control of the constitutive 35s promoter, was introduced into cotton through genetic transformation. our data showed that the expression of the lc gene alone is sufficient to trigger the accumulation of anthocyanin in a var ... | 2017 | 26941851 |
proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress. | drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. to determine the effects of drought stress on cotton seedlings, we used tw ... | 2016 | 26941218 |
transgenic cotton plants expressing cry1ia12 toxin confer resistance to fall armyworm (spodoptera frugiperda) and cotton boll weevil (anthonomus grandis). | gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including cry toxins, proteinase inhibitors, and toxic peptides, among others. in the present study, we developed transgenic cotton plants by fertilized floral buds injection (through ... | 2016 | 26925081 |
global analysis of the developmental dynamics of gossypium hirsutum based on strand-specific transcriptome. | cotton is an economically important crop that provides both natural fiber and by-products such as oil and protein. its global gene expression could provide insight into the biological processes underlying growth and development, which involve suites of genes expressed with temporal and spatial control by regulatory networks. generally, the goal for cotton breeding is improvement of the fiber; thus, most previous research has focused on identifying genes specific to the fiber. however, seeds may ... | 2016 | 26892265 |
diversity in betasatellites associated with cotton leaf curl disease during source-to-sink movement through a resistant host. | cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. the disease symptoms are induced by dna satellite known as cotton leaf curl multan betasatellite (clcumub), dominant betasatellite in cotton but another betasatellite known as chili leaf curl betasatellite (chlcb) is also found associated with the disease. grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cot ... | 2016 | 26889114 |
mapping by sequencing in cotton (gossypium hirsutum) line md52ne identified candidate genes for fiber strength and its related quality attributes. | three qtl regions controlling three fiber quality traits were validated and further fine-mapped with 27 new single nucleotide polymorphism (snp) markers. transcriptome analysis suggests that receptor-like kinases found within the validated qtls are potential candidate genes responsible for superior fiber strength in cotton line md52ne. fiber strength, length, maturity and fineness determine the market value of cotton fibers and the quality of spun yarn. cotton fiber strength has been recognized ... | 2016 | 26883043 |
relationship between potassium fertilization and nitrogen metabolism in the leaf subtending the cotton (gossypium hirsutum l.) boll during the boll development stage. | the nitrogen (n) metabolism of the leaf subtending the cotton boll (lscb) was studied with two cotton (gossypium hirsutum l.) cultivars (simian 3, low-k tolerant; siza 3, low-k sensitive) under three levels of potassium (k) fertilization (k0: 0 g k2o plant(-1), k1: 4.5 k2o plant(-1) and k2: 9.0 g k2o plant(-1)). the results showed that total dry matter increased by 13.1-27.4% and 11.2-18.5% under k supply for simian 3 and siza 3. boll biomass and boll weight also increased significantly in k1 an ... | 2016 | 26874296 |
h2o2 and aba signaling are responsible for the increased na+ efflux and water uptake in gossypium hirsutum l. roots in the non-saline side under non-uniform root zone salinity. | non-uniform root salinity increases the na(+)efflux, water use, and growth of the root in non-saline side, which may be regulated by some form of signaling induced by the high-salinity side. however, the signaling and its specific function have remained unknown. using a split-root system to simulate a non-uniform root zone salinity in gossypium hirsutum l., we showed that the up-regulated expression of sodium efflux-related genes (sos1, sos2, pma1, and pma2) and water uptake-related genes (pip1 ... | 2016 | 26862153 |
fruiting branch k(+) level affects cotton fiber elongation through osmoregulation. | potassium (k) deficiency in cotton plants results in reduced fiber length. as one of the primary osmotica, k(+) contributes to an increase in cell turgor pressure during fiber elongation. therefore, it is hypothesized that fiber length is affected by k deficiency through an osmotic pathway, so in 2012 and 2013, an experiment was conducted to test this hypothesis by imposing three potassium supply regimes (0, 125, 250 kg k ha(-1)) on a low-k-sensitive cultivar, siza 3, and a low-k-tolerant cultiv ... | 2016 | 26834777 |
comparative fiber property and transcriptome analyses reveal key genes potentially related to high fiber strength in cotton (gossypium hirsutum l.) line md52ne. | individual fiber strength is an important quality attribute that greatly influences the strength of the yarn spun from cotton fibers. fiber strength is usually measured from bundles of fibers due to the difficulty of reliably measuring strength from individual cotton fibers. however, bundle fiber strength (bfs) is not always correlated with yarn strength since it is affected by multiple fiber properties involved in fiber-to-fiber interactions within a bundle in addition to the individual fiber s ... | 2016 | 26833213 |
small interfering rnas from bidirectional transcripts of ghmml3_a12 regulate cotton fiber development. | natural antisense transcripts (nats) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. in this research, we investigated the function of small rna derived from a nat in fiber cell development. using a map-based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene (n1 ) encoding a mybmixta-like transcription factor 3 (mml3)/ghmyb25-like in chromosome a12, ... | 2016 | 26832840 |
functional characterization of a dihydroflavanol 4-reductase from the fiber of upland cotton (gossypium hirsutum). | dihydroflavanol 4-reductase (dfr) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (pas)) biosynthesis, however it is not characterized in cotton yet. in present reports, a dfr cdna homolog (designated as ghdfr1) was cloned from developing fibers of upland cotton. silencing ghdfr1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and pas. more interestingly, based on lc-ms analysis, two pa monomers, (-)-epi ... | 2016 | 26821011 |
field-based high-throughput plant phenotyping reveals the temporal patterns of quantitative trait loci associated with stress-responsive traits in cotton. | the application of high-throughput plant phenotyping (htpp) to continuously study plant populations under relevant growing conditions creates the possibility to more efficiently dissect the genetic basis of dynamic adaptive traits. toward this end, we employed a field-based htpp system that deployed sets of sensors to simultaneously measure canopy temperature, reflectance, and height on a cotton (gossypium hirsutum l.) recombinant inbred line mapping population. the evaluation trials were conduc ... | 2016 | 26818078 |
a cotton mirna is involved in regulation of plant response to salt stress. | the present study functionally identified a new microrna (microrna ovual line 5, mirnvl5) with its target gene ghchr from cotton (gossypium hirsutum). the sequence of mirnvl5 precursor is 104 nt long, with a well developed secondary structure. ghchr contains two dc1 and three phd cys/his-rich domains, suggesting that ghchr encodes a zinc-finger domain-containing transcription factor. mirnvl5 and ghchr express at various developmental stages of cotton. under salt stress (50-400 mm nacl), mirnvl5 ... | 2016 | 26813144 |
influence of dual-bt protein corn on bollworm, helicoverpa zea (boddie), survivorship on bollgard ii cotton. | similar cry proteins are expressed in both bt corn, zea mays l., and cotton, gossypium hirsutum (l.), commercial production systems. at least one generation of corn earworm, helicoverpa zea (boddie), completes development on field corn in the mid-south before dispersing across the landscape into other crop hosts like cotton. a concern is that bt corn hybrids may result in selection for h. zea populations with a higher probability of causing damage to bt cotton. the objective of this study was to ... | 2016 | 26809264 |
two-dimensional gel electrophoresis-based analysis provides global insights into the cotton ovule and fiber proteomes. | proteomic analysis of upland cotton was performed to profile the global detectable proteomes of ovules and fibers using two-dimensional electrophoresis (2de). a total of 1,203 independent protein spots were collected from representative 2de gels, which were digested with trypsin and identified by matrix-assisted laser desorption and ionization-time-offlight/ time-of-flight (maldi-tof/tof) mass spectrometry. the mass spectrometry or tandem mass spectrometry (ms or ms/ms) data were then searched a ... | 2016 | 26803300 |
cotton ghmyb7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic arabidopsis. | the secondary cell wall in mature cotton fibers contains over 90% cellulose with low quantities of xylan and lignin. however, little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers. in this study, we characterized an r2r3-myb transcription factor, ghmyb7, in cotton. ghmyb7 is expressed at a high level in developing fibers and encodes a myb protein that is targeted to the cell nucleus and has transcriptional activation activity. ectopic expression of ghmyb7 ... | 2016 | 26803299 |
genome-wide transcriptomic and proteomic analyses of bollworm-infested developing cotton bolls revealed the genes and pathways involved in the insect pest defence mechanism. | cotton bollworm, helicoverpa armigera, is a major insect pest that feeds on cotton bolls causing extensive damage leading to crop and productivity loss. in spite of such a major impact, cotton plant response to bollworm infection is yet to be witnessed. in this context, we have studied the genome-wide response of cotton bolls infested with bollworm using transcriptomic and proteomic approaches. further, we have validated this data using semi-quantitative real-time pcr. comparative analyses have ... | 2016 | 26799171 |
online co2 and h2 o oxygen isotope fractionation allows estimation of mesophyll conductance in c4 plants, and reveals that mesophyll conductance decreases as leaves age in both c4 and c3 plants. | mesophyll conductance significantly, and variably, limits photosynthesis but we currently have no reliable method of measurement for c4 plants. an online oxygen isotope technique was developed to allow quantification of mesophyll conductance in c4 plants and to provide an alternative estimate in c3 plants. the technique is compared to an established carbon isotope method in three c3 species. mesophyll conductance of c4 species was similar to that in the c3 species measured, and declined in both ... | 2016 | 26778088 |
confamiliar transferability of simple sequence repeat (ssr) markers from cotton (gossypium hirsutum l.) and jute (corchorus olitorius l.) to twenty two malvaceous species. | cross-species transferability is a quick and economic method to enrich ssr database, particularly for minor crops where little genomic information is available. however, transferability of ssr markers varies greatly between species, genera and families of plant species. we assessed confamiliar transferability of ssr markers from cotton (gossypium hirsutum) and jute (corchorus olitorius) to 22 species distributed in different taxonomic groups of malvaceae. all the species selected were potential ... | 2016 | 28330135 |
partial dominance, overdominance, epistasis and qtl by environment interactions contribute to heterosis in two upland cotton hybrids. | based on two recombinant inbred line (ril) populations, two corresponding backcross (bc) populations were constructed to elucidate the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). the yield, and yield components, of these populations were evaluated in three environments. at the single-locus level, 78 and 66 quantitative trait loci (qtl) were detected using composite interval mapping in ril and bc populations, respectively, and 29 qtl were identified based on mid-parental ... | 2015 | 26715091 |
atrav1 and atrav2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering. | there is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. to better understand drought stress signaling and adaptation in cotton (gossypium hirsutum) fiber development, we expressed the arabidopsis transcription factors related_to_aba-insensitive3/viviparous1/(rav1) and atrav2, which encode apetala2-basic3 domain proteins shown to repress transcription of flowering_locus_t (ft) and to promote stomatal opening cell-autonomously. in three years o ... | 2015 | 26706061 |
polyamine and its metabolite h2o2 play a key role in the conversion of embryogenic callus into somatic embryos in upland cotton (gossypium hirsutum l.). | the objective of this study was to increase understanding about the mechanism by which polyamines (pas) promote the conversion of embryogenic calli (ec) into somatic embryos in cotton (gossypium hirsutum l.). we measured the levels of endogenous pas and h2o2, quantified the expression levels of genes involved in the pas pathway at various stages of cotton somatic embryogenesis (se), and investigated the effects of exogenous pas and h2o2 on differentiation and development of ec. putrescine (put), ... | 2015 | 26697030 |
delineating the glycoproteome of elongating cotton fiber cells. | the data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and n-linked glycosylation site identification (kumar et al., 2013) [1]. utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. in this context we have reanalyzed our glycoproteome dataset with the gossypium arbor ... | 2015 | 26693171 |
microarray-based large scale detection of single feature polymorphism in gossypium hirsutum l. | microarrays offer an opportunity to explore the functional sequence polymorphism among different cultivars of many crop plants. the affymetrix microarray expression data of five genotypes of gossypium hirsutum l. at six different fibre developmental stages was used to identify single feature polymorphisms (sfps). the background corrected and quantile-normalized log2 intensity values of all probes of triplicate data of each cotton variety were subjected to sfps call by using sam procedure in r la ... | 2015 | 26690522 |
partial dominance, overdominance and epistasis as the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). | determination of genetic basis of heterosis may promote hybrid production in upland cotton (gossypium hirsutum l.). this study was designed to explore the genetic mechanism of heterosis for yield and yield components in f2: 3 and f2: 4 populations derived from a hybrid 'xinza no. 1'. replicated yield field trials of the progenies were conducted in 2008 and 2009. phenotypic data analyses indicated overdominance in f1 for yield and yield components. additive and dominance effects at single-locus l ... | 2015 | 26618635 |
susceptibility to insecticides and activities of glutathione s-transferase and esterase in populations of lygus lineolaris (hemiptera: miridae) in mississippi. | lygus lineolaris (palisot de beauvois) is a serious pest of cotton (gossypium hirsutum l.) in mississippi, particularly in the delta region. this may be due to decreased insecticide susceptibility in that region. research has revealed populations of l. lineolaris in the delta region with high levels of insecticide resistance; however, comparisons with populations in the remainder of the state are limited. | 2016 | 26598042 |
stable transformation and expression of ghexpa8 fiber expansin gene to improve fiber length and micronaire value in cotton. | cotton fiber is multigenic trait controlled by number of genes. previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. in the present study, the gossypium hirsutum ghexpa8 fiber expansin gene was introduced into local cotton variety niab 846 by using an agrobacterium-mediated gene transformation. the neomycin phosphotransferase (nptii) gene was used as a selection marker for scre ... | 2015 | 26583018 |
a and d genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant. | chromosomal dispositions were analyzed on the metaphase plate of tetraploid cotton (aadd). at metaphase, the two subgenomes, a and d, were separated in a radial pattern in which the small d subgenome chromosomes tended to concentrate at the center and the large a subgenome chromosomes were scattered about the periphery on the metaphase plate. although the ordered chromosome arrangement was disturbed in an artificial hexaploid (aaddgg), the separation pattern could be recovered after the majority ... | 2015 | 26568399 |
integrated mapping and characterization of the gene underlying the okra leaf trait in gossypium hirsutum l. | diverse leaf morphology has been observed among accessions of gossypium hirsutum, including okra leaf, which has advantages and disadvantages in cotton production. the okra leaf locus has been mapped to chromosome 15 of the dt subgenome, but the underlying gene has yet to be identified. in this study, we used a combination of targeted association analysis, f2 population-based fine mapping, and comparative sequencing of orthologues to identify a candidate gene underlying the okra leaf trait in g. ... | 2016 | 26567355 |
the cotton wrky gene ghwrky41 positively regulates salt and drought stress tolerance in transgenic nicotiana benthamiana. | wrky transcription factors constitute a very large family of proteins in plants and participate in modulating plant biological processes, such as growth, development and stress responses. however, the exact roles of wrky proteins are unclear, particularly in non-model plants. in this study, gossypium hirsutum wrky41 (ghwrky41) was isolated and transformed into nicotiana benthamiana. our results showed that overexpression of ghwrky41 enhanced the drought and salt stress tolerance of transgenic ni ... | 2015 | 26562293 |
stable integration and expression of a cry1ia gene conferring resistance to fall armyworm and boll weevil in cotton plants. | boll weevil is a serious pest of cotton crop. effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. the current genetically modified bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. this work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools. | 2016 | 26558603 |
molecular cloning, expression analysis and subcellular localization of a transparent testa 12 ortholog in brown cotton (gossypium hirsutum l.). | transparent testa 12 (tt12) is a kind of transmembrane transporter of proanthocyanidins (pas), which belongs to a membrane-localized multidrug and toxin efflux (mate) family, but the molecular basis of pas transport is still poorly understood. here, we cloned a full-length tt12 cdna from the fiber of brown cotton (gossypium hirsutum), named ghtt12 (genbank accession no. kf240564), which comprised 1733 bp with an open reading frame (orf) of 1503 bp and encoded a putative protein containing 500 am ... | 2016 | 26548815 |
analysis of upland cotton (gossypium hirsutum) response to verticillium dahliae inoculation by transcriptome sequencing. | verticillium wilt is one of the main diseases in cotton (gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. at present, the molecular mechanism that confers resistance to this disease is unclear. transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. in this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with verticillium da ... | 2015 | 26535625 |
microrna and mrna expression profiling analysis revealed the regulation of plant height in gossypium hirsutum. | dwarf cottons are more resistant to damage from wind and rain and associated with stable, increased yields, and also desirable source for breeding the machine harvest varieties. in an effort to uncover the transcripts and mirna networks involved in plant height, the transcriptome and small rna sequencing were performed based on dwarf mutant ari1327 (a1), tall-culm mutant ari3697 (a3) and wild type ari971 (a9) in gossypium hirsutum. | 2015 | 26517985 |
a bil population derived from g. hirsutum and g. barbadense provides a resource for cotton genetics and breeding. | to provide a resource for cotton genetics and breeding, an interspecific hybridization between gossypium hirsutum cv. emian22 and g. barbadense acc. 3-79 was made. a population of 54 bils (backcross inbred lines, bc1f8) was developed with the aim of transferring g. barbadense genes into g. hirsutum in order to genetically analyze these genes' function in a g. hirsutum background and create new germplasms for breeding. preliminary investigation of the morphological traits showed that the bils had ... | 2015 | 26517274 |
characterization and development of chloroplast microsatellite markers for gossypium hirsutum, and cross-species amplification in other gossypium species. | cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. sixty chloroplast microsatellites were identified and characterized from the complete sequence of the gossypium hirsutum chloroplast genome using a bioinformatic approach. twenty chloroplast microsatellite loci were polymorphic in the 66 gossypium germplasm accessions. a total of 85 alleles were detected, with allele numbers varying from 2-7 per locus. po ... | 2015 | 26505340 |
ghnac12, a neutral candidate gene, leads to early aging in cotton (gossypium hirsutum l). | nac (nam, ataf, and cuc) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. currently, 77 nac genes have been reported in cotton (gossypium hirsutum l.). and ghnac12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. in the present study, a preliminary function analysis of ghnac12 was performed during leaf senescence. qrt-pcr a ... | 2016 | 26496006 |
leafy cotyledon1-casein kinase i-tcp15-phytochrome interacting factor4 network regulates somatic embryogenesis by regulating auxin homeostasis. | somatic embryogenesis (se) is an efficient tool for the propagation of plant species and also, a useful model for studying the regulatory networks in embryo development. however, the regulatory networks underlying the transition from nonembryogenic callus to somatic embryos during se remain poorly understood. here, we describe an upland cotton (gossypium hirsutum) casein kinase i gene, ghcki, which is a unique key regulatory factor that strongly affects se. overexpressing ghcki halted the format ... | 2015 | 26491146 |
overexpression of ghwrky27a reduces tolerance to drought stress and resistance to rhizoctonia solani infection in transgenic nicotiana benthamiana. | wrky proteins constitute transcriptional regulators involved in various biological processes, especially in coping with diverse biotic and abiotic stresses. however, in contrast to other well-characterized wrky groups, the functions of group iii wrky transcription factors are poorly understood in the economically important crop cotton (gossypium hirsutum). in this study, a group iii wrky gene from cotton, ghwrky27a, was isolated and characterized. our data indicated that ghwrky27a localized to t ... | 2015 | 26483697 |
cotton square morphology offers new insights into host plant resistance to cotton fleahopper (hemiptera: miridae) in upland cotton. | cotton fleahopper, pseudatomoscelis seriatus (reuter) (hemiptera: miridae), is a piercing-sucking pest of cotton (gossypium hirsutum l.) that feeds preferentially on developing flower buds, called squares. heavy infestations cause yield reductions that result from abscission of squares damaged by the cotton fleahopper feeding. antixenosis, or nonpreference, has been reported as a mechanism of host plant resistance in cotton to cotton fleahopper. square structure, particularly the placement of th ... | 2016 | 26475922 |
evaluating pilose, a cultigen of gossypium hirsutum, as a source of resistance to cotton fleahopper (hemiptera: miridae). | cotton fleahopper (pseudatomoscelis seriatus reuter) (hemiptera: miridae) is a piercing-sucking insect that has emerged as a major pest of cotton (gossypium hirsutum l.) in texas. cotton fleahoppers feed on floral buds, commonly referred to as squares, causing damage and abscission, and subsequent yield loss. previous studies indicate that plant resistance to cotton fleahopper is present in upland cotton, but the mechanism of resistance remains undetermined. in this study, pilose, a cultigen of ... | 2015 | 26470352 |
in silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers. | diversity of colors in flowers and fruits is largely due to anthocyanin pigments. the flavonoid/anthocyanin pathway has been most extensively studied. dihydroflavonol 4-reductase (dfr) is a vital enzyme of the flavonoid pathway which displays major impact on the formation of anthocyanins, flavan 3-ols and flavonols. the substrate specificity of the dfr was found to play a crucial role in determination of type of anthocyanidins. altering the flavonoid/anthocyanin pathway through genetic engineeri ... | 2015 | 26442064 |
photosynthetic characteristics of the subtending leaf of cotton boll at different fruiting branch nodes and their relationships with lint yield and fiber quality. | to investigate photosynthetic characteristics of the subtending leaf at the 2-3rd and 10-11th fruiting branch (fbn, fb2-3, and fb10-11), and their relationship with cotton yield and quality, field experiments were conducted using two cotton cultivars, kemian 1 and sumian 15. the results showed that with fbn increasing, chlorophyll (chl) components, pn and non-photochemical quenching (npq) in the subtending leaf significantly declined, while soluble sugar, amino acid and their ratio (c ss/c aa) a ... | 2015 | 26442060 |
identification of early salt stress responsive proteins in seedling roots of upland cotton (gossypium hirsutum l.) employing itraq-based proteomic technique. | soil salinity is a major abiotic stress that limits plant growth and agricultural productivity. upland cotton (gossypium hirsutum l.) is highly tolerant to salinity; however, large-scale proteomic data of cotton in response to salt stress are still scant. here, an isobaric tag for relative and absolute quantitation (itraq)-based proteomic technique was employed to identify the early differentially expressed proteins (deps) from salt-treated cotton roots. one hundred and twenty-eight deps were id ... | 2015 | 26442045 |
cadmium (cd) localization in tissues of cotton (gossypium hirsutum l.), and its phytoremediation potential for cd-contaminated soils. | phytoremediation using economically valuable, large biomass, non-edible plants is a promising method for metal-contaminated soils. this study investigated cotton's tolerance for cd and remediation potential through analyzing cd bioaccumulation and localization in plant organs under different soil cd levels. results showed cotton presents good tolerance when soil cd concentration ≤20.26 mg kg(-1). cotton had good cd accumulation ability under low soil cd levels (<1.26 mg kg(-1)), with a tf value ... | 2015 | 26419249 |
herbicide-resistant cotton (gossypium hirsutum) plants: an alternative way of manual weed removal. | cotton yield has been badly affected by different insects and weed competition. in past application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. the control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. to utilise the transgenic approach, cotton plants exp ... | 2015 | 26383095 |
overexpression of a cotton (gossypium hirsutum) wrky gene, ghwrky34, in arabidopsis enhances salt-tolerance of the transgenic plants. | soil salinity is one of the most serious threats in world agriculture, and often influences cotton growth and development, resulting in a significant loss in cotton crop yield. wrky transcription factors are involved in plant response to high salinity stress, but little is known about the role of wrky transcription factors in cotton so far. in this study, a member (ghwrky34) of cotton wrky family was functionally characterized. this protein containing a wrky domain and a zinc-finger motif belong ... | 2015 | 26332661 |
comparisons of lygus lineolaris (hemiptera: miridae) populations from two distinct geographical regions of mississippi. | the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), is a major pest of cotton (gossypium hirsutum l.) in the state of mississippi. economic data indicate that l. lineolaris is a more serious pest of cotton in the delta region of mississippi than in the hills region; however, little data exist comparing the two populations. two experiments were undertaken to compare l. lineolaris from these two geographically distinct regions. in the first experiment, colonies of ... | 2015 | 26313997 |
influence of crop management and environmental factors on wolf spider assemblages (araneae: lycosidae) in an australian cotton cropping system. | wolf spiders (lycosidae) are the most abundant ground-hunting spiders in the australian cotton (gossypium hirsutum l.) agroecosystems. these spiders have potential in controlling pest bollworms, helicoverpa spp. (lepidoptera: noctuidae) in minimum-tilled fields. a study was carried out during a wet growing season (2011-2012) in narrabri, new south wales, australia, to determine how different crop rotations and tillage affect wolf spider assemblages in cotton fields. spider abundance and species ... | 2015 | 26308820 |
a high-density ssr genetic map constructed from a f2 population of gossypium hirsutum and gossypium darwinii. | the cultivated allotetraploid species gossypium hirsutum, accounts for 90% of the world cotton production, has narrow genetic basis that's why its yield, quality or stress resistance breeding is stagnant. it is therefore, essential to explore desirable genes from gossypium darwinii which has enviable traits such as high fiber fineness, drought tolerance, fusarium and verticillium resistance. we used g. darwinii as primary plant materials in this study not only to enrich the genetic diversity of ... | 2015 | 26275937 |
high resolution consensus mapping of quantitative trait loci for fiber strength, length and micronaire on chromosome 25 of the upland cotton (gossypium hirsutum l.). | cotton (gossypium hirsutum l.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. technological developments in the textile industry and improvements in human living standards have increased the requirement for supplies and better quality cotton. upland cotton 0-153 is an elite cultivar harboring strong fiber strength genes. to conduct quantitative trait locus (qtl) mapping for fiber quality in 0-153, we developed a population of 19 ... | 2015 | 26262992 |
transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress. | salinity stress is one of the most devastating abiotic stresses in crop plants. as a moderately salt-tolerant crop, upland cotton (gossypium hirsutum l.) is a major cash crop in saline areas and a suitable model for salt stress tolerance research. in this study, we compared the transcriptome changes between the salt-tolerant upland cotton cultivar zhong 07 and salt-sensitive cultivar zhong g5 in response to nacl treatments. transcriptional regulation, signal transduction and secondary metabolism ... | 2015 | 26259172 |
construction of bac contig maps of homoeologous chromosomes a12 and d12 of gossypium hirsutum l. acc. tm-1. | the gossypium hirsutum homoeologous chromosome 12 encodes important genes that contribute to fiber fuzz, lethality, gland development and male sterility. in this study a physical map of the cotton tm-1 chromosome 12 was constructed. a number of large-insert cotton genome libraries are available, and genome-wide physical mapping using large insert segments combined with bacterial cloning is a thriving area of genome research. however, sequencing of the cotton genome is difficult due to sequence r ... | 2015 | 26221184 |
the mitochondrial malate dehydrogenase 1 gene ghmmdh1 is involved in plant and root growth under phosphorus deficiency conditions in cotton. | cotton, an important commercial crop, is cultivated for its natural fibers, and requires an adequate supply of soil nutrients, including phosphorus, for its growth. soil phosporus exists primarily in insoluble forms. we isolated a mitochondrial malate dehydrogenase (mdh) gene, designated as ghmmdh1, from gossypium hirsutum l. to assess its effect in enhancing p availability and absorption. an enzyme kinetic assay showed that the recombinant ghmmdh1 possesses the capacity to catalyze the intercon ... | 2015 | 26179843 |
effect of the postfeeding interval on olfactory responses of thrips to herbivore-induced cotton plants. | we investigated the responses of 3 thrips species, frankliniella schultzei trybom, f. occidentalis pergrande, and thrips tabaci lindeman (thysanoptera: thripidae) to herbivore-damaged and undamaged cotton seedlings (gossypium hirsutum l. [malvales: malvaceae]) at a range of time intervals following damage by adult tetranychus urticae (koch), adult t. ludeni (zacher) (acari: tetranychidae) or helicoverpa armigera (hübner) (lepidoptera: noctuidae) larvae in olfactometer assays. the intensity/frequ ... | 2016 | 26172119 |
dynamic transcriptome analysis and volatile profiling of gossypium hirsutum in response to the cotton bollworm helicoverpa armigera. | in response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. however, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (cbw; helicoverpa armigera) larv ... | 2015 | 26148847 |
promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton flowering locus t (ft)-like gene ghft1. | flowering locus t (ft) encodes a mobile signal protein, recognized as major component of florigen, which has a central position in regulating flowering, and also plays important roles in various physiological aspects. a mode is recently emerging for the balance of indeterminate and determinate growth, which is controlled by the ratio of ft-like and terminal flower 1 (tfl1)-like gene activities, and has a strong influence on the floral transition and plant architecture. orthologs of ghft1 was pre ... | 2015 | 26136765 |
predawn respiration rates during flowering are highly predictive of yield response in gossypium hirsutum when yield variability is water-induced. | respiratory carbon evolution by leaves under abiotic stress is implicated as a major limitation to crop productivity; however, respiration rates of fully expanded leaves are positively associated with plant growth rates. given the substantial sensitivity of plant growth to drought, it was hypothesized that predawn respiration rates (rpd) would be (1) more sensitive to drought than photosynthetic processes and (2) highly predictive of water-induced yield variability in gossypium hirsutum. two stu ... | 2015 | 26125121 |
development of molecular markers for genetic male sterility in gossypium hirsutum. | genetic male sterility (gms) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. sequence analysis on ms5 and ms6 loci in gossypium hirsutum was conducted to reveal genomic variation at these two loci between gms and wild-type g. hirsutum inbred lines, and sequence polymorphism linked to ms5 on a12 and ms6 on d12 was revealed. a haplotype marker set that consisted of four snps targeting both ms5 and ms6 gene r ... | 2017 | 26074724 |
the complexity of posttranscriptional small rna regulatory networks revealed by in silico analysis of gossypium arboreum l. leaf, flower and boll small regulatory rnas. | micrornas (mirnas) and secondary small interfering rnas (principally phased sirnas or trans-acting sirnas) are two distinct subfamilies of small rnas (srnas) that are emerging as key regulators of posttranscriptional gene expression in plants. both mirnas and secondary-sirnas (sec-sirnas) are processed from longer rna precursors by dicer-like proteins (dcls). gossypium arboreum l., also known as tree cotton or asian cotton, is a diploid, possibly ancestral relative of tetraploid gossypium hirsut ... | 2015 | 26070200 |
a new synthetic amphiploid (aaddaa) between gossypium hirsutum and g. arboreum lays the foundation for transferring resistances to verticillium and drought. | gossypium arboreum, a cultivated cotton species (2n = 26, aa) native to asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. however, it is quite difficult to transfer favorable traits into upland cotton through conventional methods due to the cross-incompatibility of g. hirsutum (2n = 52, aadd) and g. arboreum. here, we improved an embryo rescue technique to overcome the ... | 2015 | 26061996 |
itraq-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.). | male sterility is a common phenomenon in flowering plants, and it has been successfully developed in several crops by taking advantage of heterosis. cotton (gossypium hirsutum l.) is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to use ccri9106 in cotton hybrid breeding, it is of great importance t ... | 2015 | 26047712 |
genetic fine mapping and candidate gene analysis of the gossypium hirsutum ligon lintless-1 (li1) mutant on chromosome 22(d). | ligon lintless-1 (li1) is a gossypium hirsutum mutant that is controlled by a dominant gene that arrests the development of cotton fiber after anthesis. two f2 mapping populations were developed from mutant (li1 × h7124) f1 plants in 2012 and 2013; each was composed of 142 and 1024 plants, respectively. using these populations, li1 was mapped to a 0.3-cm region in which nine single-strand conformation polymorphism markers co-segregated with the li1 locus. in the published g. raimondii genome, th ... | 2015 | 26037218 |
mapping-by-sequencing of ligon-lintless-1 (li 1 ) reveals a cluster of neighboring genes with correlated expression in developing fibers of upland cotton (gossypium hirsutum l.). | mapping-by-sequencing and snp marker analysis were used to fine map the ligon-lintless-1 ( li 1 ) short fiber mutation in tetraploid cotton to a 255-kb region that contains 16 annotated proteins. the ligon-lintless-1 (li 1 ) mutant of cotton (gossypium hirsutum l.) has been studied as a model for cotton fiber development since its identification in 1929; however, the causative mutation has not been identified yet. here we report the fine genetic mapping of the mutation to a 255-kb region that co ... | 2015 | 26021293 |
inheritance and qtl mapping of leaf nutrient concentration in a cotton inter-specific derived ril population. | developing and deploying cotton cultivars with high nutrient uptake, use efficiency and tolerance to nutrient related soil stresses is desirable to assist sustainable soil management. genetic variation, heritability, selection response and quantitative trait loci (qtls) were investigated for five macronutrients (p, k, ca, mg, s) and five micronutrients (fe, mn, b, zn, and cu) in a recombinant inbred line (ril) population from an inter-specific cross between gossypium hirsutum cv. guazuncho 2, an ... | 2015 | 26020945 |