| nitrogen partitioning and isotopic fractionation in dairy cows consuming diets based on a range of contrasting forages. | nine multiparous holstein-friesian cows (initially 97 d in milk), were used in a 3×3 lattice square design experiment with 4-wk periods. all cows received 4 kg/d concentrates and dietary treatments were based on silages offered ad libitum: perennial ryegrass (prg); timothy (tim); tall fescue (tf); red clover (rc); red clover/corn silage mixture [40/60 on a dry matter (dm) basis; rcc]; red clover/whole-crop oat silage mixture (40/60 on a dm basis; rco); or red clover/whole-crop oat silage mixture ... | 2011 | 21426994 |
| proteomics reveals potential biomarkers of seed vigor in sugarbeet. | to unravel biomarkers of seed vigor, an important trait conditioning crop yield, a comparative proteomic study was conducted with sugarbeet seed samples of varying vigor as generated by an invigoration treatment called hydropriming and an aging treatment called controlled deterioration. comparative proteomics revealed proteins exhibiting contrasting behavior between seed samples. thus, 18 proteins were up-regulated during priming and down-regulated during aging and further displayed an up-regula ... | 2011 | 21432998 |
| [effect of acholeplasma laidlawii var. granulum on lectin activity of sugar beet calluses]. | the dynamics of lectin activity of sugar beet calluses infected by mollicute acholeplasma laidlawii var granulum str. 118 was studied. activity of acid-soluble lectins of sugar beet cell cultures increased 4.5 times during some first hours after infecting by acholeplasma. at early stages of infecting by mollicute qualitative changes were also revealed in the protein spectra of acid-soluble lectins of sugar beet calluses. | 2011 | 21442949 |
| selection of bacteria capable of dissimilatory reduction of fe(iii) from a long-term continuous culture on molasses and their use in a microbial fuel cell. | ferric ion-respiring microorganisms (frms) are a group of prokaryotes that use fe(iii) as well as other metals as terminal electron acceptors in the process of anaerobic respiration. special attention is paid to a biotechnological significance of frms because of their potential role in electricity production in microbial fuel cells (mfcs) where the terminal acceptor of the electrons during anaerobic respiration is not a ferric ion but the anode. one of the best known frms is the shewanellaceae f ... | 2011 | 21464603 |
| anaerobic-fed and sequencing-batch treatment of sugar-beet processing wastes: a comparative study. | the aim of this study was to compare a batch-fed continuously mixed anaerobic reactor (fcmr) with an anaerobic sequencing batch reactor (asbr), in terms of waste stabilization and methane production treating sugar-beet processing wastewater and beet-pulp simultaneously. a reactor was operated as fcmr, which then was operated as an asbr by changing operational conditions after the steady-state was reached. although the hydraulic retention time value of the asbr configuration was lower (8 days) th ... | 2011 | 21466072 |
| application of plant-based antimicrobials for the growth inhibition of clostridia in pressed beet pulp silage. | background: in this study the inhibition of hop beta acids on the growth of clostridia in soil-contaminated pressed sugar beet pulp silages was investigated. hop beta acids are natural substances which display their effect at low concentrations. fresh pressed beet pulp material was mixed with soil to artificially contaminate it with clostridia. laboratory silos were filled with the substrate, stored at 25 °c and opened for sampling at 0, 2, 8, 15, 30, 60, and 90 days. the impact on clostridial g ... | 2011 | 21495041 |
| expression and substrate specificity of betaine/proline transporters suggest a novel choline transport mechanism in sugar beet. | proline transporters (prots) originally described as highly selective transporters for proline, have been shown to also transport glycinebetaine (betaine). here we examined and compared the transport properties of bet/prots from betaine accumulating (sugar beet, amaranthus, and atriplex,) and non-accumulating (arabidopsis) plants. using a yeast mutant deficient for uptake of proline and betaine, it was shown that all these transporters exhibited higher affinity for betaine than proline. the upta ... | 2011 | 21511362 |
| anaerobic co-digestion of by-products from sugar production with cow manure. | sugar beet leaves (sbl), sugar beet top (sbt), sugar beet pulp (sbp) and desugared molasses (dm) are by-products from the sugar production. in the present study we investigated the potential of sbl, sbt and sbp as feedstock for biogas production. the maximum methane potential of sbl, sbt and sbp determined by batch assays was found to be 490, 500 and 240 ml-ch(4)/gvs-added respectively. three reactor experiments were carried out to investigate the effect of co-digestion of sbp, dm and manure at ... | 2011 | 21530994 |
| distribution of airborne mycosphaerella graminicola inoculum at the field scale. | a network of 10 burkard 7-day spore-recording traps was set up in the walloon region in belgium to monitor the airborne inoculum of wheat pathogens. three spore traps were used to analyse the distribution of mycosphaerella graminicola inoculum at the field scale, at 1 m above ground level. two traps were set up in a wheat field 100 m apart. the third trap was placed 70 m away in a sugar beet field adjacent to the wheat field. total dna from each fragment of spore trap tape corresponding to 1 day ... | 2010 | 21534470 |
| arabidopsis thaliana, a new tool to investigate polymyxa betae-host interactions. | little is known about the genome of polymyxa betae and its interactions with sugar beet, due partly to the obligate nature of the protist and the patents on beta vulgaris sequences. the identification of an ecotype of arabidopsis thaliana compatible with the protist would help to improve this knowledge. the infection and development of p. betae in 14 worldwide ecotypes of a. thaliana were studied. the detection of plasmodia and resting spores and the production of zoospores in the roots of a. th ... | 2010 | 21542467 |
| phytostabilization of semiarid soils residually contaminated with trace elements using by-products: sustainability and risks. | we investigated the efficiency of various by-products (sugarbeet lime, biosolid compost and leonardite), based on single or repeated applications to field plots, on the establishment of a vegetation cover compatible with a stabilization strategy on a multi-element (as, cd, cu, pb and zn) contaminated soil 4-6 years after initial amendment applications. results indicate that the need for re-treatment is amendment- and element-dependent; in some cases, a single application may reduce trace element ... | 2011 | 21561696 |
| feed preference in pigs: effect of selected protein, fat, and fiber sources at different inclusion rates. | three double choice feeding experiments were conducted to study the effect of different feedstuffs on feed preference in pigs. fifteen protein sources, 6 fat sources, and 3 fiber sources were evaluated in exp. 1, 2, and 3, respectively. pigs were offered a series of double choices between a common reference diet and the diet with the ingredient under evaluation. the reference diet contained a soybean meal product with 56% cp (sbm-56), sunflower oil, and wheat bran, which were considered as the f ... | 2011 | 21571898 |
| feruloylated and nonferuloylated arabino-oligosaccharides from sugar beet pectin selectively stimulate the growth of bifidobacterium spp. in human fecal in vitro fermentations. | the side chains of the rhamnogalacturonan i fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. in this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by amberlite xad hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (dp 2-10) and long-chain (dp 7-14) feruloylated and nonf ... | 2011 | 21574556 |
| [activity of plant cells oxidative enzymes in conditions of experimental mycoplasmosis]. | changes in activity of oxidative enzymes was shown when infecting the callus culture of sugar beet cells by phytopathogenic mollicute acholeplasma laidlawii var granulum 118. maximum indices of the enzyme activity were fixed 3 hours after infecting with regard to control: it was 49% for peroxidase, 38% for catalase and 45% for polyfenoloxidase. the decrease of enzymatic activity to 22%, 12% and 19%, respectively, was registered 5 hours after infecting, and after that all these indices became sta ... | 2011 | 21598658 |
| prospects for using nonconventional feeds in diets for awassi dairy sheep in syria. | high feed costs are major obstacles for resource-poor dairy sheep farmers in west asia, along with large fluctuation in grain and straw prices. farmers need low-cost diets using locally available feeds that can provide sufficient milk of good quality. two experimental trials were conducted on awassi milking ewes to evaluate nonconventional and balanced low-cost diets against the traditional unbalanced diet used by farmers (control) on the total yields (milk, fat, protein, and total solids) and m ... | 2011 | 21605771 |
| pathogenicity, symptom development, and mycotoxin formation in wheat by fusarium species frequently isolated from sugar beet. | crop rotations with putative non-host crops like sugar beet are often recommended to reduce fusarium head blight (fhb) in cereals. however, recent observations have shown pathogenic, endophytic, and saprotrophic colonization of sugar beet with various fusarium species. therefore, strains of seven species frequently isolated from sugar beet were tested for pathogenicity on wheat. species-specific symptoms on heads and kernels were evaluated and the grains were analyzed for 17 mycotoxins with lc-m ... | 2011 | 21635142 |
| [experimental food-induced fatty liver and its adjuvant therapy with natural bioactive substances]. | changes of redox-homeostasis generate cytokines, and free radicals influence many intracellular signaling pathways in different liver diseases. liophylised table beet and carrot powder (gps powder kft. 1361/004/2003bfáééá) containing bioactive components such as betaine, betanins, betaxanthins, flavonoids, polyphenols, glutamine, beta carotene, vitamins and folic acid may induce changes in various cellular pathways. | 2011 | 21652297 |
| addition of genes for cellobiase and pectinolytic activity in escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass. | ethanologenic escherichia coli strain ko11 was sequentially engineered to contain the klebsiella oxytoca cellobiose phosphotransferase genes (casab) as well as a pectate lyase (pele) from erwinia chrysanthemi, yielding strains ly40a (casab) and jp07 (casab pele), respectively. to obtain an effective secretion of pele, the sec-dependent pathway out genes from e. chrysanthemi were provided on a cosmid to strain jp07 to construct strain jp07c. finally, oligogalacturonide lyase (ogl) from e. chrysan ... | 2011 | 21666025 |
| identification of a sugar beet bvm14-mads box gene through differential gene expression analysis of monosomic addition line m14. | monosomic addition line m14 carrying an additional chromosome 9 from beta corolliflora zosimovic ex buttler was obtained through hybridization between the wild species b. corolliflora and a cultivated species beta vulgaris l. var saccharifera alef. the m14 line showed diplosporic reproduction and stress tolerance. to identify differentially expressed genes in m14, a subtractive cdna library was prepared by suppression subtractive hybridization (ssh) between m14 (2n=18+1) and b. vulgaris (2n=18). ... | 2011 | 21807438 |
| transgenic sugar beet tolerant to imidazolinone obtained by agrobacterium-mediated transformation. | sugar beet is highly sensitive to imidazolinone herbicides thus rotational restrictions exist. in order to develop imidazolinone tolerant sugar beets als gene from arabidopsis thaliana encoding acetolactate synthase with s653n mutation was used for genetic transformation. transgenic sugar beet plants were obtained by agrobacterium-mediated transformation of aseptic seedlings using vacuum-infiltration. the efficiency of genetic transformation was 5.8%. rt-pcr analysis of obtained plants revealed ... | 2011 | 21774399 |
| detection and characterization of spontaneous internal deletion mutants of beet necrotic yellow vein virus rna3 from systemic host nicotiana benthamiana. | beet necrotic yellow vein virus (bnyvv) is a member of the genus benyvirus causing a worldwide sugar beet disease rhizomania. bnyvv contains four or five plus-sense single stranded rnas. in altered selective conditions, multipartite rna viruses of plant are prone to undergoing internal deletions, thus turning into defective rnas (d rnas). although several d rnas have been reported in bnyvv infection, the spontaneous internal deletion mutants responsible for severe symptom in systemic host nicoti ... | 2011 | 21718549 |
| amelioration of d-galactosamine-induced acute liver injury in rats by dietary supplementation with betaine derived from sugar beet molasses. | the effects of betaine supplementation on d-galactosamine-induced liver injury were examined in terms of hepatic and serum enzyme activities and of the levels of glutathione and betaine-derived intermediates. the rats induced with liver injury showed marked increases in serum enzyme activity, but those receiving dietary supplementation of 1% betaine showed enzyme activity levels similar to a control group without liver injury. administration of betaine also increased both hepatic and serum gluta ... | 2011 | 21737928 |
| in vitro dual culture of polymyxa betae in agrobacterium rhizogenes transformed sugar beet hairy roots in liquid media. | abstract. polymyxa betae is a soil-borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus. sugar beet hairy roots, transformed by agrobacterium rhizogenes, were inoculated with surface-sterilized root fragments infected by p. betae. after 10ôçâwk in a liquid medium, typical structures of p. betae were observed in this in vitro system. this first in vitro culture of p. betae in liquid medium will contribute to a better understanding of this protist ... | 2011 | 21699623 |
| Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: Substrate specificity, polymerizing properties and usage of different acceptors for fructosylation. | Levansucrases of Pseudomonas syringae pv. tomato DC3000 (Lsc3) and Pseudomonas chlororaphis subsp. aurantiaca (also Pseudomonas aurantiaca) (LscA) have 73% identity of protein sequences, similar substrate specificity and kinetic properties. Both enzymes produce levan and fructooligosaccharides (FOS) of varied length from sucrose, raffinose and sugar beet molasses. A novel high-throughput chip-based nanoelectrospray mass spectrometric method was applied to screen alternative fructosyl acceptors f ... | 2011 | 21820018 |
| Comparative analysis of hydrogen-producing bacterial biofilms and granular sludge formed in continuous cultures of fermentative bacteria. | A system for biohydrogen production was developed based on long-term continuous cultures grown on sugar beet molasses in packed bed reactors. In two separate cultures, consortia of fermentative bacteria developed as biofilms on granitic stones. In one of the cultures, a granular sludge was also formed. Metagenomic analysis of the microbial communities by 454-pyrosequencing of amplified 16S rDNA fragments revealed that the overall biodiversity of the hydrogen-producing cultures was quite small. T ... | 2011 | 21908188 |
| The effect of acetylated xylan and sugar beet pulp on the expression and secretion of enzymes by Penicillium purpurogenum. | Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus and to purify different enzymes secreted to the medium. The enzymes involved in the complex process of degradation of these carbon sources by the fungus have been explored previous ... | 2011 | 22159660 |
| characterization of cbcyp51 from field isolates of cercospora beticola. | the hemi-biotrophic fungus cercospora beticola causes leaf spot of sugarbeet. leaf spot control measures include the application of sterol demethylation inhibitor (dmi) fungicides. however, reduced sensitivity to dmis has been reported recently in the red river valley sugarbeet growing region of north dakota and minnesota. here, we report the cloning and molecular characterization of cbcyp51, which encodes the dmi target enzyme sterol p450 14α-demethylase in c. beticola. cbcyp51 is a 1,632 b ... | 2011 | 22085297 |
| Dietary supplementation with nonconventional feeds from the Middle East: Assessing the effects on physicochemical and organoleptic properties of Awassi sheep milk and yogurt. | Increased feed costs affect the livelihoods of dairy sheep farmers in the Middle East. Farmers endure high risks with large fluctuations in the price of grain used as animal feed, which is further affected by drought and declining range productivity. Using agricultural by-products and treated straw or vetch grazing for supplementing sheep diets would provide resource-poor dairy farmers with increased options to reduce feed costs, but the effects of such feeds on the quality of yogurt (the main p ... | 2011 | 22118064 |
| extracellular virb5 enhances t-dna transfer from agrobacterium to the host plant. | virb5 is a type 4 secretion system protein of agrobacterium located on the surface of the bacterial cell. this localization pattern suggests a function for virb5 which is beyond its known role in biogenesis and/or stabilization of the t-pilus and which may involve early interactions between agrobacterium and the host cell. here, we identify virb5 as the first agrobacterium virulence protein that can enhance infectivity extracellularly. specifically, we show that elevating the amounts of the extr ... | 2011 | 22028781 |
| effects of the inclusion of sodium bicarbonate and sugar beet pulp in the concentrate for fattening lambs on acid-base status and meat characteristics. | thirty-six young merino lambs (15.3kg live weight) were allocated into four equal groups to study the effects of the inclusion of sodium bicarbonate ('bic', 0 vs. 2%) and sugar beet pulp ('sbp', 0 vs. 12%) in the concentrate on meat characteristics. lambs were fed barley straw and concentrate ad libitum. when they reached 25kg a blood sample was taken and the animal slaughtered. after 24h meat characteristics (ph, colour, water holding capacity, warner-bratzler shear force, chemical and fatty ac ... | 2007 | 22061960 |
| the ferulic acid esterases of chrysosporium lucknowense c1: purification, characterization and their potential application in biorefinery. | three ferulic acid esterases from the filamentous fungus chrysosporium lucknowense c1 were purified and characterized. the enzymes were most active at neutral ph and temperatures up to 45 °c. all enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. ferulic acid esterases faea1 and faea2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oli ... | 2012 | 22133444 |
| Fungicide resistance assays for fungal plant pathogens. | Fungicide resistance assays are useful to determine if a fungal pathogen has developed resistance to a fungicide used to manage the disease it causes. Laboratory assays are used to determine loss of sensitivity, or resistance, to a fungicide and can explain fungicide failures and for developing successful fungicide recommendations in the field. Laboratory assays for fungicide resistance are conducted by measuring reductions in growth or spore germination of fungi in the presence of fungicide, or ... | 2012 | 22183666 |
| beet soil-borne mosaic virus rna-4 encodes a 32kda protein involved in symptom expression and in virus transmission through polymyxa betae. | beet soil-borne mosaic virus (bsbmv), like beet necrotic yellow vein virus (bnyvv), is a member of the benyvirus genus and both are transmitted by polymyxa betae. both viruses possess a similar genomic organization: rna-1 and -2 are essential for infection and replication while rna-3 and -4 play important roles in disease development and vector-mediated infection in sugar beet roots. we characterized a new species of bsbmv rna-4 that encodes a 32kda protein and a chimeric form of bsbmv rna-3 and ... | 2011 | 22209119 |
| independent origins of vectored plant pathogenic bacteria from arthropod-associated arsenophonus endosymbionts. | the genus arsenophonus (gammaproteobacteria) is comprised of intracellular symbiotic bacteria that are widespread across the arthropods. these bacteria can significantly influence the ecology and life history of their hosts. for instance, arsenophonus nasoniae causes an excess of females in the progeny of parasitoid wasps by selectively killing the male embryos. other arsenophonus bacteria have been suspected to protect insect hosts from parasitoid wasps or to expand the host plant range of phyt ... | 2011 | 21892672 |
| sugar-beet pulp as an alternative ingredient of barley in rabbit diets and its effect on rabbit meat. | the effects of rabbit diets with four different levels of barley and sugar-beet pulp on the dressing percentage, chemical and fatty acid composition of meat from rabbits slaughtered at a liveweight of 2000 and 2500 g have been studied. results revealed detectable (p < 0·05) effects of the total substitution of barley by sugar-beet pulp for carcass weight, dressing percentage and dry matter from animals slaughtered at a liveweight of 2000 g. no significant differences (p > 0·05) among batches wer ... | 1995 | 22059768 |
| relationships between skatole and androstenone accumulation, and cytochrome p4502e1 expression in meishan×large white pigs. | the effects of age, castration and diet on accumulation of skatole and androstenone in the backfat of 50% meishan male pigs, was studied in relation to boar taint. skatole and androstenone deposition in backfat of entire males was increased with age (114, 144 and 174 days). castration significantly decreased skatole and androstenone levels in all age groups. the level of cytochrome p4502e1, the initial enzyme involved in hepatic skatole metabolism, was increased by castration at all ages. these ... | 2004 | 22061806 |
| Comparative digestibility of energy and nutrients in fibrous feed ingredients fed to Meishan and Yorkshire pigs. | The objective of this experiment was to test the hypothesis that differences in the digestibility of total dietary fiber (TDF) among breeds of pigs is influenced by the type of fiber fed and also by the age of the pig. Five Meishan pigs (BW: 77.2 ± 15.2 kg; 5 mo old), 5 light Yorkshire pigs (BW: 80.1 ± 11.2 kg; 4 mo old), and 5 heavy Yorkshire pigs (BW: 102.1 ± 3.5 kg, 5 mo old) were surgically prepared with a T-cannula in the distal ileum. A corn-soybean meal diet (control) was formulated with ... | 2011 | 21984712 |
| Ethanol production from sugar beet molasses by S. cerevisiae entrapped in an alginate-maize stem ground tissue matrix. | A new alginate-maize stem ground tissue matrix was developed as a Saccharomyces cerevisiae carrier for ethanol fermentation from sugar beet molasses. There were several fermentation procedures in the present study: using free cells and alginate-entrapped cells with and without maize stem ground tissue supplementation (F; F+C; AB; AB+C), and using a new combined alginate-maize stem ground tissue carrier (ABC). It was found that addition of maize stem ground tissue meal (C), with honeycomb configu ... | 2011 | 22112953 |
| Molecular identification of two strains of Cercospora rodmanii isolated from water hyacinth present in Yuriria lagoon, Guanajuato, Mexico and identification of new hosts for several other strains. | Water hyacinth is a beautiful monocotyledon plant that has been dispersed all over the world by humans. The plant has been present in Mexico since 1907, and many water bodies have become infested with it since then. In 2001, we initiated a survey in Yuriria lagoon in southern Guanajuato state to isolate fungi able to biocontrol the plant. We isolated 25 morphologically distinct fungal cultures, of which two were identified as members of the genus Cercospora. Cercospora species are among the most ... | 2011 | 22036293 |
| carcass quality in male fallow deer (dama dama): effects of age and supplementary feeding. | thirty-two male fallow deer, half 18- and half 30-month-old, were slaughtered after a 4-month feeding trial on pasture alone or with daily supplement of 500 g (dry matter) concentrate/head (eight each 18- and 30-month-old). the concentrate was composed of 40% maize, 25% sugar beet pulp, 20% alfalfa, 13% soy flakes, 2% minerals and vitamins. supplemented deer were heavier (49.9 vs 45.0 kg) and their carcasses produced higher dressing proportions (61.5 vs 57.2%) and bigger amounts of first quality ... | 2002 | 22063647 |
| l-Arabinose production from sugar beet arabinan by immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus in a packed-bed reactor. | The immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus produced continuously an average of 16.5gl(-1)l-arabinose from 20gl(-1) sugar beet arabinan at pH 5.0 and 75°C for 216h, with a productivity of 9.9gl(-1)h(-1) and a conversion yield of 83%. | 2011 | 22099373 |
| [construction of a bivalent plant expression vector carrying vvsuc11 and vvsuc12 genes and its genetic transformation in sugar beet]. | we have recombined genes vvsuc11, vvsuc12 from vitis vinifera l., and root-specific promoters of sweet potato storage protein gene from ipomoea batatas l. lam., named as sp1 and sp2. we have constructed a vector pcambia2301-sp1- vvsuc11-sp2-vvsuc12 using pcambia2301 as an original vector. vvsuc11 and vvsuc12 were under the control of root-specific promoters of sweet potato storage protein gene. we transformed the vector into kws-9103 breeding line of beta vulgaris l. with agrobacterium-mediated ... | 2011 | 22097805 |
| adaptation of rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics. | abstract: background: the rhizosphere is the microbe-rich zone around plant roots and is a key determinant of the biosphere's productivity. comparative transcriptomics was used to investigate general and plant-specific adaptations during rhizosphere colonization. rhizobium leguminosarum biovar viciae was grown in the rhizospheres of pea (its legume nodulation host), alfalfa (a non-host legume) and sugar beet (non-legume). gene expression data were compared to metabolic and transportome maps to ... | 2011 | 22018401 |
| In vitro fermentation of sugar beet arabino-oligosaccharides by fecal microbiota obtained from patients with ulcerative colitis to selectively stimulate the growth of Bifidobacterium spp. and Lactobacillus spp. | The potential prebiotic properties of arabino-oligosaccharides (AOS) derived from sugar beet pulp was studied using mixed cultures of human fecal bacteria from patients with ulcerative colitis (UC), in remission or with active disease, and in healthy controls. These results were compared to those for fructo-oligosaccharides (FOS), which are known to have a prebiotic effect. Fermentation studies were carried out using a small-scale static batch system, and changes in the fecal microbial communiti ... | 2011 | 21984234 |
| growth, carcass and meat characteristics of karayaka growing rams fed sugar beet pulp, partially substituting for grass hay as forage. | twenty-one growing male karayaka sheep were divided into three groups of seven at 12 months of age. all groups received a commercial feed (60%) and roughage (40%). one group received grass hay as roughage (control, c), the other two groups received a mixture in which 40 (0.40sbp) or 60% (0.60sbp) sugar beet pulp (sbp) was used in partial replacement of grass hay in the c group during 56 days. dry matter (dm) intake and dm conversion index (kg dm per kg weight gain) of the c group were higher (p< ... | 2005 | 22063275 |
| metabolic effects of dietary sugar beet pulp or wheat bran in growing female pigs. | an experiment was conducted to determine the effects of feeding a moderate level of 2 different fiber sources on energy metabolites; mitochondrial biogenesis in the intestine, liver, and muscle; and the expression of some genes that regulate energy metabolism in intestine, liver, muscle, and adipose tissue. female pigs (n = 36; bw = 15.0 ± 0.7 kg) were fed diets containing no added fiber, 12.5% sugar beet pulp (sbp), or 12.5% wheat bran (wb) for 24 d. blood samples were collected on d 7 and 24 f ... | 2011 | 21926324 |
| process model and economic analysis of ethanol production from sugar beet raw juice as part of the cleaner production concept. | the batch fermentation process of sugar beet processing intermediates by free yeast cells is the most widely used method in the autonomous province of vojvodina for producing ethanol as fuel. in this study a process and cost model was developed for producing ethanol from raw juice. the model can be used to calculate capital investment costs, unit production costs and operating costs for a plant producing 44millionl of 99.6% pure ethanol annually. in the sensitivity analysis the influence of suga ... | 2012 | 22104094 |
| the prevalence of 'candidatus arsenophonus phytopathogenicus' infecting the planthopper pentastiridius leporinus (hemiptera: cixiidae) increase nonlinearly with the population abundance in sugar beet fields. | the planthopper pentastiridius leporinus (l.) (hemiptera: cixiidae) has been identified as the main vector of 'candidatus arsenophonus phytopathogenicus', a plant pathogenic bacterium associated to a sugar beet disease in eastern france called syndrome 'basses richesses'. in a 2-yr survey (2006-07), we quantified the abundance of p. leporinus populations migrating into 29 sugar beet fields in eastern france. sticky traps posted in these fields were monitored on a twice-weekly (2006) or weekly ( ... | 2011 | 22217748 |
| lime pretreatment of sugar beet pulp and evaluation of synergy between arfa, mana and xyna from clostridium cellulovorans on the pretreated substrate. | sugar beet pulp (sbp) is a waste product from the sugar beet industry and could be used as a potential biomass feedstock for second generation biofuel technology. pretreatment of sbp with 'slake lime' (calcium hydroxide) was investigated using a 2(3) factorial design and the factors examined included lime loading, temperature and time. the pretreatment was evaluated for its ability to enhance enzymatic degradation using a combination of three hemicellulases, namely arfa (an arabinofuranosidase), ... | 2011 | 22611526 |
| identification and precise mapping of resistant qtls of cercospora leaf spot resistance in sugar beet (beta vulgaris l.). | the complex inheritance of resistance to cercospora leaf spot (cls), the most severe fungal foliar disease in sugar beet, was investigated by means of quantitative trait loci (qtl) analysis. over a three year period, recombinant inbred lines (rils) of sugar beet (beta vulgaris l.), generated through a cross between lines resistant ('nk-310mm-o') and susceptible ('nk-184mm-o') to cls, were field-tested for their resistance to the pathogen. composite interval mapping (cim) showed four qtl involved ... | 2011 | 22384339 |
| identification of beet necrotic yellow vein virus in lakes district: a major beet growing area in turkey. | this study was conducted to determine the prevalence of beet necrotic yellow vein virus (bnyvv) causal agent of rhizomania, in sugar beet cultivation areas in the lakes district of turkey, in 2006-2007. during surveys bnyvv suspected leaf, root, and soil samples were collected. total of 203 soil samples were collected from different locations in the region. sugar beet cultivar kasandra plants were grown in these soil samples using bait plant techniques. in order to detect the structures of the v ... | 2011 | 23637514 |
| cloning, expression and medium optimization of validamycin glycosyltransferase from streptomyces hygroscopicus var. jinggangensis for the biotransformation of validoxylamine a to produce validamycin a using free resting cells. | validamycin a is widely used to control basidiomycetes, which causes sheath blight disease in rice, potatoes, vegetables, and other crops as well as dumping-off disease in vegetable seedlings, cotton, sugar beets, and other plants. in order to improve the content of validamycin a in the commercial products, valg from streptomyces hygroscopicus was successfully cloned into escherichia coli bl21(de3) and was directly employed as the biocatalyst in the biotransformation from validoxylamine a to val ... | 2012 | 23340099 |
| effects of dietary supplementation with betaine on a nonalcoholic steatohepatitis (nash) mouse model. | the effects of betaine supplementation on non-alcoholic steatohepatitis (nash) model mice were examined by measuring the accumulation of fat in the livers of nash model mice compared to a control. betaine from sugar beets was provided to the model mice as a dietary supplement. after 3 wk of dietary supplementation, there were no significant differences in body weight or liver weight between the groups. however, the liver to body weight ratio in the high-fat diet with betaine (hfb) group was sign ... | 2012 | 23327974 |
| high level resistance against rhizomania disease by simultaneously integrating two distinct defense mechanisms. | with the aim of achieving durable resistance against rhizomania disease of sugar beet, the employment of different sources of resistance to beet necrotic yellow vein virus was pursued. to this purpose, nicotiana benthamiana transgenic plants that simultaneously produce dsrna originating from a conserved region of the bnyvv replicase gene and the hrpz(psph) protein in a secreted form (sp/hrpz(psph)) were produced. the integration and expression of both transgenes as well as proper production of t ... | 2012 | 23284692 |
| fusarium culmorum: causal agent of foot and root rot and head blight on wheat. | fusarium culmorum is a ubiquitous soil-borne fungus able to cause foot and root rot and fusarium head blight on different small-grain cereals, in particular wheat and barley. it causes significant yield and quality losses and results in contamination of the grain with mycotoxins. this review summarizes recent research activities related to f. culmorum, including studies into its population diversity, mycotoxin biosynthesis, mechanisms of pathogenesis and resistance, the development of diagnostic ... | 2012 | 23279114 |
| systemic resistance induced by bacillus lipopeptides in beta vulgaris reduces infection by the rhizomania disease vector polymyxa betae. | the control of rhizomania, one of the most important diseases of sugar beet caused by the beet necrotic yellow vein virus, remains limited to varietal resistance. in this study, we investigated the putative action of bacillus amylolequifaciens lipopeptides in achieving rhizomania biocontrol through the control of the virus vector polymyxa betae. some lipopeptides that are produced by bacteria, especially by plant growth-promoting rhizobacteria, have been found to induce systemic resistance in pl ... | 2012 | 23279057 |
| effects of replacing dietary starch with neutral detergent-soluble fibre on ruminal fermentation, microbial synthesis and populations of ruminal cellulolytic bacteria using the rumen simulation technique (rusitec). | a rumen simulation technique (rusitec) apparatus with eight 800 ml fermenters was used to investigate the effects of replacing dietary starch with neutral detergent-soluble fibre (ndsf) by inclusion of sugar beet pulp in diets on ruminal fermentation, microbial synthesis and populations of ruminal cellulolytic bacteria. experimental diets contained 12.7, 16.4, 20.1 or 23.8% ndsf substituted for starch on a dry matter basis. the experiment was conducted over two independent 15-day incubation peri ... | 2012 | 23278844 |
| a study on cations and color removal from thin sugar juice by modified sugar beet pulp. | this article describes the use of citric acid modified sugar beet pulp as new ion-exchanger sorbent for the removal of metal cations and colorants from thin juice. the results of batch adsorption runs concerning the effects of contact time, material dosage, temperature and ph drop were presented and discussed. experimental data on the removal of metal cations showed that the sorption process was rapid and reached equilibrium in 60 min. modified material in acidic form caused to a significant ph ... | 2011 | 23729851 |
| the molecular basis of vernalization in different plant groups. | timing of flowering is key to the reproductive success of many plants. in temperate climates, flowering is often coordinated with seasonal environmental cues such as temperature and photoperiod. vernalization, the process by which a prolonged exposure to the cold of winter results in competence to flower during the following spring, is an example of the influence of temperature on the timing of flowering. in different groups of plants, there are distinct genes involved in vernalization, indicati ... | 2012 | 23619014 |
| response of plant parasitic and free living soil nematodes to composted animal manure soil amendments. | in an outside pot experiment, dry pig manure processed on pine sawdust litter and fermented for seven days by house fly larvae (fermented manure), and pine sawdust applied alone, and in combination with a spring application of inorganic nitrogen fertilizer were used to determine their effects on plant parasitic and free-living soil nematodes on sugar beets (cv. antek). non amended soil was used as a control. all treatments with fermented pig manure and sawdust with nitrogen fertilizer decreased ... | 2012 | 23482503 |
| population dynamics of dactylella oviparasitica and heterodera schachtii: toward a decision model for sugar beet planting. | a series of experiments were performed to examine the population dynamics of the sugarbeet cyst nematode, heterodera schachtii, and the nematophagus fungus dactylella oviparasitica. after two nematode generations, the population densities of h. schachtii were measured in relation to various initial infestation densities of both d. oviparasitica and h. schachtii. in general, higher initial population densities of d. oviparasitica were associated with lower final population densities of h. schacht ... | 2012 | 23481664 |
| tailor-made fructan synthesis in plants: a review. | fructan, a fructose polymer, is produced by many bacteria and plants. fructan is used as carbohydrate reserve, and in bacteria also as protective outside layer. chicory is a commercial fructan producing crop. the disadvantage of this crop is its fructan breakdown before harvest. studies using genetically modification showed that fructan biosynthesis is difficult to steer in chicory. alternatives for production of tailor-made fructan, fructan with a desired polymer length and linkage type, are or ... | 2012 | 23465900 |
| fate of chloroalkylene-9-14c in carrots, sugar beets, and soil under outdoor conditions. | immediately after application of chloroalkylene-9-14c to soil (1.32 ppm, based on dry weight of soil in the upper layer of 0 to 10 cm) under outdoor conditions, carrots were sown; in the following year, sugar beets were grown. about 80% of the radioactivity applied volatilized within one vegetation period. most of the remaining radioactivity was still in the upper soil layer; 0.8% had dispersed to a depth of 40 cm, and 3.3% was taken up by the carrot plants. in the second year, no more decreases ... | 2002 | 120137 |
| [sensitization to the pollen of sugar beet]. | | 2015 | 4849687 |
| nadp dependent oxidation of tdp-glucose by an enzyme system from sugar beets. | | 2015 | 4381717 |
| the use of ba(oh) 2 in methanol for demonstration of the distribution of sucrose in sugarbeet roots. | | 2016 | 5019002 |
| asymmetric somatic cell hybridization in plants. ii. electrophoretic analysis of radiation-induced dna damage and repair following the exposure of sugarbeet (beta vulgaris l.) protoplasts to uv and gamma rays. | as part of an investigation into whether it would be possible to use uv radiation as a suitable pretreatment of the donor cells in asymmetric hybridization experiments, the effects of this treatment on sugarbeet (beta vulgaris l.) protoplast dna have been determined and compared with those of gamma radiation. both nuclear and mitochondrial dnas have been examined. the dose ranges chosen had previously been determined to be potentially applicable for fusion experiments. pulsed field gel electroph ... | 2006 | 1508156 |
| soil populations of rhizoctonia solani from areas of health and diseased beets within four sugarbeet fields differing in soil texture. | both debris isolation and beet seed colonization methods were used to ascertain rhizoctonia solani populations in areas of healthy (ah) and rhizoctonia crown rot diseased (da) sugarbeets within four fields differing in soil texture over a 1-year period (august 1976 to july 1977). inoculum densities were initially (august-october) higher in da than in ah, but declined over the winter to levels similar to ah by june. as ascertained by the debris isolation method, ah populations remained low (mostl ... | 1990 | 526886 |
| microbial aggregates in anaerobic wastewater treatment. | the phenomenon aggregation of anaerobic bacteria gives an opportunity to speed up the digestion rate during methanogenesis. the aggregates are mainly composed of methanogenic bacteria which convert acetate and h2/co2 into methane. other bacteria are also included in the aggregates but their concentration is rather small. the aggregates may also be formed during acetogenesis or even hydrolysis but such aggregates are not stable and disrupt quickly when not fed. a two stage process seems to be sui ... | 2008 | 2291438 |
| heterogeneity of circular mitochondrial dna molecules from sugar beet with normal and male sterile cytoplasms. | mitochondrial (mt) dnas from normal (n) and male sterile (s) cytoplasms of sugar beet have been isolated and investigated by electron microscopy. the results showed that mtdna was composed of a heterogeneous population of circular molecules. their contour lengths varied from 0.28 to 51 micron, but unlike in the case of maize, a large difference was not observed in the distribution of molecular classes greater than 1.0 micron between n and s cytoplasms of sugar beet. on the other hand, n and s cy ... | 2012 | 3447745 |
| fate of aldrin-14c in sugar beets and soil under outdoor conditions. | | 2015 | 4733378 |
| inhibition of plant calcium-dependent protein kinases by basic polypeptides. | wheat embryo ca2+-dependent protein kinase (cdpk) is inhibited by a variety of polypeptides including actin, gramicidin s, melittin, protamine, various histone preparations, histone h4 and by basic amino-acid homopolymers. melittin (ki 9 microm) is a non-competitive inhibitor of wheat germ cdpk and also inhibits wheat leaf cdpk and silver beet leaf cdpks. protamine inhibits wheat germ cdpk in an apparently competitive fashion (ki 0.2 microm) and is also a potent, albeit less effective, inhibitor ... | 1982 | 2306477 |
| comparative evaluation of the enzyme multiplicity in a diploid, a triploid and a tetraploid sugar beet variety. | | 1991 | 1263460 |
| genome sequence reveals that pseudomonas fluorescens f113 possesses a large and diverse array of systems for rhizosphere function and host interaction. | pseudomonas fluorescens f113 is a plant growth-promoting rhizobacterium (pgpr) isolated from the sugar-beet rhizosphere. this bacterium has been extensively studied as a model strain for genetic regulation of secondary metabolite production in p. fluorescens, as a candidate biocontrol agent against phytopathogens, and as a heterologous host for expression of genes with biotechnological application. the f113 genome sequence and annotation has been recently reported. | 2013 | 23350846 |
| the complete nucleotide sequence of the mitochondrial genome of sugar beet (beta vulgaris l.) reveals a novel gene for trna(cys)(gca). | we determined the complete nucleotide sequence of the mitochondrial genome of an angiosperm, sugar beet (beta vulgaris cv tk81-o). the 368 799 bp genome contains 29 protein, five rrna and 25 trna genes, most of which are also shared by the mitochondrial genome of arabidopsis thaliana, the only other completely sequenced angiosperm mitochondrial genome. however, four genes identified here (namely rps13, trnf-gaa, ccb577 and trnc2-gca) are missing in arabidopsis mitochondria. in addition, four gen ... | 2000 | 10871408 |
| diversity and activity of lysobacter species from disease suppressive soils. | the genus lysobacter includes several species that produce a range of extracellular enzymes and other metabolites with activity against bacteria, fungi, oomycetes, and nematodes. lysobacter species were found to be more abundant in soil suppressive against the fungal root pathogen rhizoctonia solani, but their actual role in disease suppression is still unclear. here, the antifungal and plant growth-promoting activities of 18 lysobacter strains, including 11 strains from rhizoctonia-suppressive ... | 2015 | 26635735 |
| protozoa drive the dynamics of culturable biocontrol bacterial communities. | some soil bacteria protect plants against soil-borne diseases by producing toxic secondary metabolites. such beneficial biocontrol bacteria can be used in agricultural systems as alternative to agrochemicals. the broad spectrum toxins responsible for plant protection also inhibit predation by protozoa and nematodes, the main consumers of bacteria in soil. therefore, predation pressure may favour biocontrol bacteria and contribute to plant health. we analyzed the effect of acanthamoeba castellani ... | 2013 | 23840423 |
| genome mining and metabolic profiling of the rhizosphere bacterium pseudomonas sp. sh-c52 for antimicrobial compounds. | the plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. previously, we isolated pseudomonas sp. sh-c52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (mendes et al., 2011). to get more insight into its biosynthetic repertoire, the genome o ... | 2015 | 26217324 |
| metagenomic analysis of the bacterial community associated with the taproot of sugar beet. | we analyzed a metagenome of the bacterial community associated with the taproot of sugar beet (beta vulgaris l.) in order to investigate the genes involved in plant growth-promoting traits (pgpts), namely 1-aminocyclopropane-1-carboxylic acid (acc) deaminase, indole acetic acid (iaa), n2 fixation, phosphate solubilization, pyrroloquinoline quinone, siderophores, and plant disease suppression as well as methanol, sucrose, and betaine utilization. the most frequently detected gene among the pgpt c ... | 2015 | 25740621 |
| osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth. | choline monooxygenase (cmo) catalyzes the committing step in the synthesis of glycine betaine, an osmoprotectant accumulated by many plants in response to salinity and drought. to investigate how these stresses affect cmo expression, a spinach (spinacia oleracea l., chenopodiaceae) probe was used to isolate cmo cdnas from sugar beet (beta vulgaris l., chenopodiaceae), a salt- and drought-tolerant crop. the deduced beet cmo amino acid sequence comprised a transit peptide and a 381-residue mature ... | 1998 | 9489025 |
| differences between the rhizosphere microbiome of beta vulgaris ssp. maritima-ancestor of all beet crops-and modern sugar beets. | the structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. therefore, we studied bacterial rhizosphere communities associated with the wild beet b. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (cs) and modern sugar beets ... | 2014 | 25206350 |
| the cyst nematode effector protein 10a07 targets and recruits host posttranslational machinery to mediate its nuclear trafficking and to promote parasitism in arabidopsis. | plant-parasitic cyst nematodes synthesize and secrete effector proteins that are essential for parasitism. one such protein is the 10a07 effector from the sugar beet cyst nematode, heterodera schachtii, which is exclusively expressed in the nematode dorsal gland cell during all nematode parasitic stages. overexpression of h. schachtii 10a07 in arabidopsis thaliana produced a hypersusceptible phenotype in response to h. schachtii infection along with developmental changes reminiscent of auxin eff ... | 2015 | 25715285 |
| the transcriptome of syncytia induced by the cyst nematode heterodera schachtii in arabidopsis roots. | arabidopsis thaliana is a host for the sugar beet cyst nematode heterodera schachtii. juvenile nematodes invade the roots and induce the development of a syncytium, which functions as a feeding site for the nematode. here, we report on the transcriptome of syncytia induced in the roots of arabidopsis. microaspiration was employed to harvest pure syncytium material, which was then used to prepare rna for hybridization to affymetrix genechips. initial data analysis showed that the gene expression ... | 2008 | 18980640 |
| hspro controls early nicotiana attenuata seedling growth during interaction with the fungus piriformospora indica. | in a previous study aimed at identifying regulators of nicotiana attenuata responses against chewing insects, a 26-nucleotide tag matching the hspro (ortholog of sugar beet hs1(pro)(-)(1)) gene was found to be strongly induced after simulated herbivory (gilardoni et al., 2010). here we characterized the function of hspro during biotic interactions in transgenic n. attenuata plants silenced in its expression (ir-hspro). in wild-type plants, hspro expression was not only induced during simulated h ... | 2012 | 22892352 |
| a sugar beet chlorophyll a/b binding protein promoter void of g-box like elements confers strong and leaf specific reporter gene expression in transgenic sugar beet. | modification of leaf traits in sugar beet requires a strong leaf specific promoter. with such a promoter, expression in taproots can be avoided which may otherwise take away available energy resources for sugar accumulation. | 2004 | 15579211 |
| alternative splicing of the maize ac transposase transcript in transgenic sugar beet (beta vulgaris l.). | the maize activator/dissociation (ac/ds) transposable element system was introduced into sugar beet. the autonomous ac and non-autonomous ds element excise from the t-dna vector and integrate at novel positions in the sugar beet genome. ac and ds excisions generate footprints in the donor t-dna that support the hairpin model for transposon excision. two complete integration events into genomic sugar beet dna were obtained by ipcr. integration of ac leads to an eight bp duplication, while integra ... | 2010 | 20512402 |
| pectin-rich biomass as feedstock for fuel ethanol production. | the usa has proposed that 30 % of liquid transportation fuel be produced from renewable resources by 2030 (perlack and stokes 2011). it will be impossible to reach this goal using corn kernel-based ethanol alone. pectin-rich biomass, an under-utilized waste product of the sugar and juice industry, can augment us ethanol supplies by capitalizing on this already established feedstock. currently, pectin-rich biomass is sold (at low value) as animal feed. this review focuses on the three most studie ... | 2012 | 22695801 |
| effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production. | this study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. the chemical composition of these feedstocks was determined. conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. in single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the con ... | 2012 | 24031984 |
| bioethanol production from fermentable sugar juice. | bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. this paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarca ... | 2014 | 24715820 |
| simultaneous saccharification and fermentation of sugar beet pulp for efficient bioethanol production. | sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. the objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (ssf) of sugar beet pulp-based worts. pressure-thermal pretreatment was applied to sugar beet pulp suspen ... | 2016 | 27722169 |
| characterization of the rumen lipidome and microbiome of steers fed a diet supplemented with flax and echium oil. | developing novel strategies for improving the fatty acid composition of ruminant products relies upon increasing our understanding of rumen bacterial lipid metabolism. this study investigated whether flax or echium oil supplementation of steer diets could alter the rumen fatty acids and change the microbiome. six hereford × friesian steers were offered grass silage/sugar beet pulp only (gs), or gs supplemented either with flax oil (gsf) or echium oil (gse) at 3% kg(-1) silage dry matter in a 3 × ... | 2014 | 25223749 |
| [effect of mycoplasma infection to fatty acid composition of callus culture sugar beet]. | it was studied the effect of acholeplasma laidlawii var. granulum str. 118 to fatty acid composition of sugar beet calluses. it was established that acting of acholeplasma results to changes in the quantitative content of the individual fatty acids and in the qualitative composition of fatty acids in the lipids of calluses. the changing of the fatty acid composition of calluses lipids of sugar beet infected by a. laidlawii vargranulum str. 118 is observed as nonspecific response to biotic stress ... | 2016 | 26829840 |
| a protocol for sonication-assisted agrobacterium rhizogenes-mediated transformation of haploid and diploid sugar beet (beta vulgaris l.) explants. | hairy root cultures obtained after agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. so far no efficient protocol of hairy root development in sugar beet has been publically released. in this work, two a. rhizogenes strains (a4t and lba1334) carrying a binary vector pbin-m-gfp5-er or pcambia1301 possessing gfp and uida reporter genes were used to transf ... | 2014 | 24660169 |
| cloning of the coat protein gene from beet necrotic yellow vein virus and its expression in sugar beet hairy roots. | expression of the beet necrotic yellow vein virus (bnyvv) coat protein (cp) gene in transgenic sugar beet hairy roots was accomplished as a step towards cp-mediated virus resistance. a cdna for the cp gene and its 5' terminal untranslated leader sequence was prepared from bnyvv rna, using two oligodeoxynucleotides to prime the synthesis of both strands. second-strand synthesis and amplification of the cdna were done by taq dna polymerase chain reactions. run-off transcripts of the cloned cdna se ... | 1991 | 24221440 |
| co-expression of xerophyte zygophyllum xanthoxylum zxnhx and zxvp1-1 confers enhanced salinity tolerance in chimeric sugar beet (beta vulgaris l.). | salinity is one of the major abiotic stresses that limit the growth and productivity of sugar beet (beta vulgaris l.). to improve sugar beet's salinity tolerance, the zxnhx and zxvp1-1 genes encoding tonoplast na(+)/h(+) antiporter and h(+)-ppase from xerophyte zygophyllum xanthoxylum were co-expressed by agrobacterium tumefaciens-mediated transformation. it is showed here that co-expression of zxnhx and zxvp1-1 confers enhanced salinity tolerance to the transformed sugar beet plants compared wi ... | 2015 | 26284097 |
| biological activities of the antiviral protein be27 from sugar beet (beta vulgaris l.). | the ribosome inactivating protein be27 displays several biological activities in vitro that could result in a broad action against several types of pathogens. beetin 27 (be27), a ribosome-inactivating protein (rip) from sugar beet (beta vulgaris l.) leaves, is an antiviral protein induced by virus and signaling compounds such as hydrogen peroxide and salicylic acid. its role as a defense protein has been attributed to its rna polynucleotide:adenosine glycosidase activity. here we tested other pu ... | 2015 | 25326773 |
| agroinoculation of beet necrotic yellow vein virus cdna clones results in plant systemic infection and efficient polymyxa betae transmission. | agroinoculation is a quick and easy method for the infection of plants with viruses. this method involves the infiltration of tissue with a suspension of agrobacterium tumefaciens carrying binary plasmids harbouring full-length cdna copies of viral genome components. when transferred into host cells, transcription of the cdna produces rna copies of the viral genome that initiate infection. we produced full-length cdna corresponding to beet necrotic yellow vein virus (bnyvv) rnas and derived repl ... | 2013 | 23384276 |
| the p25 pathogenicity factor of beet necrotic yellow vein virus targets the sugar beet 26s proteasome involved in the induction of a hypersensitive resistance response via interaction with an f-box protein. | p25, a beet necrotic yellow vein virus (bnyvv) pathogenicity factor, interacts with a sugar beet protein with high homology to arabidopsis thaliana kelch repeat containing f-box family proteins (fbk) of unknown function in yeast. fbk are members of the skp1-cullin-f-box (scf) complex that mediate protein degradation. here, we confirm this sugar beet fbk-p25 interaction in vivo and in vitro and provide evidence for in planta interaction and similar subcellular distribution in nicotiana tabacum le ... | 2012 | 22512382 |
| beet necrotic yellow vein virus coat protein-mediated protection in sugarbeet (beta vulgaris l.) protoplasts. | transformed beta vulgaris l. suspension cultures were obtained after cocultivation of sugarbeet cells with agrobacterium tumefaciens harbouring a binary vector containing the coat protein gene of beet necrotic yellow vein virus inserted between the kanamycin resistance gene and a ß-glucuronidase reporter gene. protoplasts were isolated both from untransformed cells, and from transformed cells expressing the viral coat protein, and both were then infected with beet necrotic yellow vein virus. com ... | 1990 | 24226708 |
| interspecific and host-related gene expression patterns in nematode-trapping fungi. | nematode-trapping fungi are soil-living fungi that capture and kill nematodes using special hyphal structures called traps. they display a large diversity of trapping mechanisms and differ in their host preferences. to provide insights into the genetic basis for this variation, we compared the transcriptome expressed by three species of nematode-trapping fungi (arthrobotrys oligospora, monacrosporium cionopagum and arthrobotrys dactyloides, which use adhesive nets, adhesive branches or constrict ... | 2014 | 25384908 |