| mycoplasmas and 'diphtheroids' in rheumatoid arthritis. | the failure to isolate conventional mycoplasmas from 88 synovial membranes and 119 synovial fluids from patients with proven rheumatoid arthritis using a variety of culture media and techniques agrees with the results of recent workers and suggests that these organisms are unlikely to play a role in the aetiology of the disease. in contrast, 'diphtheroid' organisms were isolated from 27 to 30% of rheumatoid specimens, but not from cultures of non-rheumatoid material. a significantly higher incid ... | 1975 | 1202604 |
| haematological changes and iron metabolism in rats after administration of corynebacterium parvum. | administration of 1-4 mg of c. parvum to rats caused a transient and mild normochromic and normocytic anaemia with reticulocytosis. there was also an immediate and marked fall of plasma iron concentration and a moderate increase of total iron binding capacity of plasma. despite increased clearance of 59fe from the plasma there was no significant change of the plasma iron turnover rate. at later intervals after administration of c. parvum an increased incorporation of iron into red blood cells an ... | 1975 | 1203175 |
| activation of the classical and alternate pathways of complement by corynebacterium parvum. | the immunological adjuvant corynebacterium parvum has been to activate the alternate pathway of complement in human and guinea-pig serum. human serum in addition contains anti-c. parvum antibodies leading to activation of the classical complement pathway. the possible role of a c. parvum derived polysaccharide in this activation is considered in relation to the biological effects of the micro-organism. | 1975 | 1204238 |
| [biosynthesis and properties of a cyclic tetrapyrrole precursor of vitamin b 12]. | | 1975 | 1204463 |
| further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. i. variation in administration of both agents. | studies from this laboratory have indicated that the administration of cyclophosphamide (cy) and corynebacterium parvum (cp) over a prolonged time to c3h mice with established measurable tumors resulted in complete arrest of tumor growth as well as partial and complete regressions in many instances. the present investigations on optimal dosage, route, frequency, and sequence of administration of cy and cp in the model system were performed to obtain information that could be useful in the design ... | 1975 | 1206740 |
| mchanisms of antitumor action of corynebacterium parvum: nonspecific tumor cell destruction at site of immunologically mediated sensitivity reaction to c. parvum. | mice immunized in the footpad with corynebacterium parvum developed a systemic mechanism with a potential for destroying syngeneic tumor cells. the development of this mechanism of antitumor action depended on the generation of a state of systemic sensitivity to c. parvum antigens, which allowed an immunologically mediated inflammatory response to be focused at the site of a tumor cell challenge or in the bed of an established tumor. this resulted in complete inhibition of growth of the former r ... | 1975 | 1206760 |
| [electrophoretic studies of proteins of propionic bacteria under different cultural conditions]. | | 1975 | 1207514 |
| [effect of nucleotide containing corrinoids on vitamin b 12 synthesis in propionibacterium shermanii]. | the influence of b12-cn, b12-oh, coenzyme b12, factor iii and factor b on the synthesis of vitamin b12 and porphyrins by different strains of p. shermanii was investigated. neither compound inhibited the development of propionic bacteria or suppressed porphyrin formation. all nucleotide containing analogues of vitamin b12 produced a strong repressive effect on the synthesis of corrinoid compounds regardless of the modifications in the upper and lower cobalt ligands. factor b containing no nucleo ... | 1975 | 1208371 |
| proceedings: therapy of methyl cholanthrene induced cba mouse tumours with corynebacterium parvum and irradiated tumour cells. | | 1975 | 1212356 |
| anaerobic and aerobic skin bacteria before and after skin-disinfection with chlorhexidine: an experimental study in volunteers. | the amount, composition, and localization of anaerobic and aerobic bacteria in the normal skin before and after disinfection were the subject of a volunteer study. the superficial bacterial flora were sampled by velvet pad imprints, and the deep flora were determined from whole skin biopsies. only one anaerobic species, propionebacterium acnes, was encountered even though other and more strict anaerobic bacteria could have been grown with the anaerobic technique employed. staphylococcus albus do ... | 1975 | 1214012 |
| effect of prolonged corynebacterium parvum and cyclophosphamide administration on spontaneously occurring metastases. | | 1975 | 1216089 |
| effect of lysozyme on in vitro digestibility of protein from the biomass of propionibacterium shermanii. | | 1975 | 1218390 |
| optimal conditions for in vitro enzymatic hydrolysis of protein from the biomass of propionibacterium shermanii by means of pronase. | | 1975 | 1218391 |
| 1h and 31p fourier transform magnetic resonance studies of the conformation of enzyme-bound propionyl coenzyme a on the transcarboxylase. | the relaxation rates of the carbon-bound protons and of the three assigned phosphorus resonances of propionyl-coa were measured in solutions of free propionyl-coa and of the transcarboxylase-propionyl-coa complex. in free propionyl-coa, analysis of the 1/t1 values of 15 protons at 100 and 220 mhz and of 1/t1 and 1/t2 of the three phosphorus atoms at 40.5 mhz indicated free rotation of the propionyl region (taur approximately 3 x 10(-11) sec) but hindered motion of the remainder of the molecule w ... | 1976 | 1247514 |
| responsiveness of senescent mice to the antitumor properties of corynebacterium parvum. | the antitumor properties of corynebacterium parvum have been studied in young (3- to 8-month-old) and aged 18 or more months old) balb/c mice given s.c., i.m., i.p., or i.v. transplants of the highly malignant, weakly immunogenic line 1 lung carcinoma, and in aged (25- to 33-month-old) balb/c mice bearing primary mammary tumors. these aged balb/c mice were shown to be less immunoresponsive than their younger counterparts, and this, in combination with nonimmunological factors, made them more sen ... | 1976 | 1247995 |
| intermolecular tritium transfer in the transcarboxylase reaction. | transfer of tritium from [3-3h]pyruvate into propionyl-coa is found during the reaction of transcarboxylase: methylmalonyl-coa + pyruvate leads to oxalacetate + propionyl-coa. about 5% of the tritium counts that are labilized in the reaction are found in a position of the propionate that exchanges rapidly with water in the presence of transcarboxylase. transfer from [2-3h]propionate of propionyl-coa to pyruvate is real but only about one-tenth as great. the tritium transfers between reactants on ... | 1976 | 1249062 |
| suppression of the incidence of death with spontaneous tumours in dba/2 mice after corynebacterium parvum-mediated rejection of syngeneic tumours. | | 1976 | 1250378 |
| studies on the fc receptor bearing cells in a transplanted methylcholanthrene induced mouse fibrosarcoma. | the presence of fc receptors on the surface of cell suspensions obtained from a transplanted isogeneic methylcholanthrene induced murine fibrosarcoma has been investigated by determining the capacity of such cells to form rosettes with antibody coated srbc. these studies indicate that a large percentage of cells in the tumour had fc receptors on their surface. the proportion of such cells was increased by reducing the number of cells transplanted, by administering cyclophosphamide to the host, a ... | 1976 | 1252329 |
| proceedings: immunological mechanisms involved in the inhibition of tumour metastases by corynebacterium parvum. | | 1976 | 1252731 |
| [on uroporphyrinogen formation: studies with 1-aminomethyl-3, 8, 13, 18-tetra(2-carboxyethyl)-2, 7, 12, 17-tetracarboxymethylbilinogen (author's transl)]. | the preparation of the aminomethyl-bilinogen which results from formal "head to tail" condensation of porphobilinogen is described. the chemical cyclocondensation of this compound at ph 7.4 yields uroporphyrinogen i. enzymatic studies with enzyme preparations from propionibacterium shermanii, which synthesize uroporphyrinogens from porphobilinogen, show that the rate of cyclisation is increased by these enzymes and indicate that the bilinogen also might be used for uroporphyrinogen iii formation ... | 1976 | 1254244 |
| antitumor activity of corynebacterium parvum extracts. | extracts of corynebacterium parvum produced by mild hydrolysis of the whole organisms had antitumor activity if given iv 1 day before iv administration of fibrosarcoma cells or if given ip or sc in admixture with these cells. a lipid component seemed responsible for these effects. unlike whole bacteria, they had little immunotherapeutic activity if given 3 days after sc tumor implantation unless absorbed onto latex. however, organisms treated with acid did not have any immunotherapeutic effect i ... | 1976 | 1255777 |
| further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. ii. effect of cortisone acetate. | studies from this laboratory have demonstrated that the administration of cyclophosphamide (cy) and corynebacterium parvum (cp) over a prolonged time to c3h mice with established measurable tumors resulted in complete arrest of tumor growth as well as partial and complete regressions in many instances. a study of the effect of two different doses of cortisone acetate (ca), administered two or five times weekly, on the tumor inhibitory properties of this chemoimmunotherapeutic regimen indicated t ... | 1976 | 1255788 |
| anaerobic infections in children: a prospective study. | the prevalence and significance of anaerobic infections in patients at st. louis children's hospital were studied prospectively for a one-year period. blood, selected body fluids, and aspirates obtained from abscesses or areas of cellulitis were cultured using special anaerobic collection and processing techniques. infected peritoneal fluid from patients with gastrointestinal disease yielded a large proportion of all anaerobic isolates. the types of organisms isolated were common inhabitants of ... | 1976 | 1256941 |
| immunotherapy and human tumor immunology. | present knowledge about the immune response to tumors in man is briefly reviewed, and the effects of cancer on immune-system functions are noted. the concepts that human tumors elicit cell-mediated immune responses to tumor antigens are re-examined and modified; for example, cell-mediated immunity may be detectable only at certain stages of tumor growth. introduction of more sensitive methods for detecting antibodies is providing additional evidence for humoral immune responses to tumor-associat ... | 1976 | 1259292 |
| effect of corynebacterium parvum on the response to irradiation of a c3h fibrosarcoma. | a methylcholanthrene-induced fibrosarcoma of c3h mice has been used as 5th- to 6th-generation syngeneic transplant in an investigation of the role of corynebacterium parvum as an adjuvant to the therapeutic effect of local irradiation of established tumors. the most effective route for administration of c. parvum in this tumor system was i.v., and the greatest effect of the c. parvum-mediated tumor graft rejection was observed for tumor growing intracutaneously or s.c. an intermediate level of e ... | 1976 | 1260756 |
| cerebrospinal fluid shunt infections with anaerobic diphtheroids (propionibacterium species). | the clinical and laboratory findings in six cases of anaerobic diphtheroid infection of cerebrospinal fluid shunts are described. these organisms have been infrequently reported as a cause of shunt infections but our data indicate that such infections may be more common than currently appreciated. propionibacterium species are common contaminants of cerebrospinal fluid specimens, but when isolated from the spinal fluid of a patient with a shunt who has symptoms and signs compatible with infecti ... | 1976 | 1262917 |
| antitumour effect of corynebacterium parvum. possible mode of action. | | 1976 | 1268035 |
| bioassay for quantitating circulating tumor cells in a syngeneic mouse tumor system. | a bioassay is described for the quantitation of tumor cells in blood specimens in a syngeneic mouse tumor system (sarcoma 1 in a/j mice). the procedure involved i.m. injection of blood containing tumor cells into each thigh of normal recipient mice and, 14 days later, examination of the sites of injection for evidence of tumor growth. for each specimen, a tumor index was calculated based on the number of tumor takes and the size of the tumors. the number of tumor cells was determined by comparis ... | 1976 | 1268858 |
| corynebacterium parvum. | | 1976 | 1269143 |
| importance of actinomyces and certain gram-negative anaerobic organisms in the transformation of lymphocytes from patients with periodontal disease. | dental plaque deposits are known to be potent stimulants of lymphocyte transformation in patients with periodontal disease but not in normal subjects. since plaque deposits consist mainly of whole bacteria, the cell walls of the most commonly found organisms in plaque were tested for their capacity to induce lymphocyte transformation. there was a direct correlation between the severity of peridontal disease and the amount of transformation induced by the cell walls of oral bacteria and by solubi ... | 1976 | 1270144 |
| modification of the effect of c. parvum on macrophage activity and tumour growth by x-irradiation. | the radiosensitivity of three forms of response to injection of c. parvum in mice has been investigated. the increase in phagocytin index evoked by ip or iv injection of 0.7 mg c. parvum (but not that evoked by 1.4 mg) was reduced but not abolished in mice given 350-500 rad whole-body irradiation 4 days before c. parvum injection. irradiation (500-1,000 rad) 4 days after c. parvum injection had no such effect. the antitumour cytotoxicity in vitro of pe and spleen cells from c. parvum-treated mic ... | 1976 | 1270180 |
| phosphorus pentoxide as a drying agent for bacterial culture extracts analyzed by gas-liquid chromatography. | the procedure for gas chromatographic analysis of metabolic products of microbial fermentation includes solvent extraction of the aqueous growth media, drying of the extract, and direct chromatographic analysis of the solvent. in this study, 2 drying agents, magnesium sulfate and phosphorus pentoxide, were compared. both were effective in removing water; however, phosphorus pentoxide removed water more completely and at a faster rate than magnesium sulfate. when a thermal conductivity detector i ... | 1976 | 1270388 |
| anaerobic infections in children: a prospective survey. | over an 18-month period, cultures from 95 infants and children yielded 146 anaerobic organisms in 110 clinical specimens. bacteroides was the most frequently isolated anaerobe, followed by propionibacterium and clostridium species. intra-abdominal sources, soft tissues, and blood were the three major sources (82%) of isolation of anaerobes. whereas most patients (58%) were over 5 years of age and only 11% were newborns, anaerobic infections constituted a rather uniform proportion of all infectio ... | 1976 | 1270594 |
| [antibacterial and bactericidal activity of tinidazole against anaerobic bacteria comparing with metronidazole (author's transl)]. | antibacterial activity of tinidazole (1-2-(ethylsulfonyl)-2-methyl-5-nitroimidazole) against anaerobic bacteria including peptococcus, peptostreptococcus, eubacterium, propionibacterium, bacteroides and fusobacterium was studied by agar dilution method comparing with metronidazole. in addition to this work, bactericidal effect of tinidazole and metronidazole against p. prevotii, b. fragilis ss. fragilis and f. varium was examined by quantitative culture method after incubation in gam broth conta ... | 1976 | 1271586 |
| anaerobic coryneforms isolated from human bone marrow and skin. chemical, biochemical and serological studies and some of their biological activities. | eighteen isolates if anaerobic coryneforms from human bone marrow and skin and four type strains of propionibacterium were studied chemically, biochemically and antigenically. all of the isolates were identified as propionibacterium acnes; of the 18 isolates,16 belonged to sterotype i and two to serotype ii. by means of gas liquid chromatography and mass spectral analysis, a large amount of iso-type fatty acids, such as iso-pentadecanoic and iso-heptadecanoic acids were detected in whole cells o ... | 1976 | 1271588 |
| treatment of cancer with immunomodulators. | | 1976 | 1271891 |
| the effects of corynebacterium parvum and surgery on the lewis lung carcinoma and its metastases. | the effects of corynebacterium parvum on the mouse primary lewis lung carcinoma and its metastases were studied. c. parvum was given at the same time as subcutaneous inoculation of tumour or in combination with surgical excision of the primary after 10 days' growth. when intravenous c. parvum was given at the same time as tumour there was a reduction in the primary tumour growth rate. there was a similar reduction in growth if the drug was given intravenously 7 days after tumour inoculation. int ... | 1976 | 1276661 |
| the effect of corynebacterium parvum in combination with 5-fluorouracil, l-phenylalanine mustard, or methotrexate on the inhibition of tumor growth. | previous reports from this laboratory have demonstrated conclusively that cyclophosphamide administered asynchronously with corynebacterium parvum (cp) results in greater c3h mammary tumor inhibition than that observed with either agent alone. an analysis of this combination has revelaed that the chemotherapeutic component contributes more significantly to tumor inhibition than does the immunotherapeutic one. this study was conducted to investigate the inhibition of c3h mammary tumors by other c ... | 1976 | 1277181 |
| nitric oxide-induced s-nitrosylation of glyceraldehyde-3-phosphate dehydrogenase inhibits enzymatic activity and increases endogenous adp-ribosylation. | using conditions that produced chronic inflammation in rat liver, we were able to find a correlation between induction of nitric oxide production and inhibition of glyceraldehyde-3-phosphate dehydrogenase (gapdh; ec 1.2.1.12). this enzyme is a tetramer composed of identical m(r) 37,000 subunits. the tetramer contains 16 thiol groups, four of which are essential for enzymatic activity. our information indicates that four thiol groups are s-nitrosylated by exposure to authentic nitric oxide (no) g ... | 1992 | 1281150 |
| identification of inducible calmodulin-dependent nitric oxide synthase in the liver of rats. | a calmodulin-dependent nitric oxide synthase was significantly induced in the liver of rats treated intravenously with heat-killed propionibacterium acnes and 5 days later with escherichia coli lipopolysaccharide. the apparent calmodulin-dependent and -independent isozymes were separated by mono q column chromatography after their partial purification by 2',5'-adp-agarose affinity chromatography. both enzymes had a molecular weight of 125,000 as determined by sds-polyacrylamide gel electrophores ... | 1992 | 1281157 |
| suppressive effects of e3330, a novel quinone derivative, on tumor necrosis factor-alpha generation from monocytes and macrophages. | e3330 [(2e)-3-[5-(2,3-dimethoxy-6-methyl-1,4-benzoquinoyl)]-2-nonyl-2- propenoic acid], a novel synthesized hepatoprotective compound, has suppressive effects on tumor necrosis factor-alpha (tnf-alpha) generation from monocytes/macrophages in vitro. e3330 (1-100 microm) reduced lipopolysaccharide (lps, 10 mg/ml or 1 microgram/ml)-induced tnf-alpha generation from rat resident and propionibacterium acnes (p. acnes)-elicited peritoneal macrophages, rat and human monocytes, rat kupffer cells, and s ... | 1992 | 1284192 |
| [solitary infected renal cyst: a case report]. | a 48-year-old man was admitted to our hospital because of high fever and left flank pain. laboratory findings revealed a high white blood cell count, high c-reactive protein level, and severe pyuria. sonographic examination revealed an enlargement of the cyst at the upper pole of the left kidney that had already been detected. percutaneous drainage was performed for the cyst and 60 ml of purulent fluid was obtained. bacterial culture of the fluid was positive for propionibacterium acnes and gamm ... | 1992 | 1288227 |
| effect of crude bacterial lipids on the course of listeria infection in mice. | crude lipids from 37 strains belonging to 32 bacterial species were isolated. by injecting mice with lipids 5 d prior to challenge with a virulent strain of listeria monocytogenes, immunostimulatory activity in 19 preparations was found. in general, lipids of gram-negative bacteria appeared to be more effective. as to bacilli, an extraordinary activity was found in the lipids of bacillus firmus. lipids of various species of the genus listeria were found to be active in approximately one-half of ... | 1992 | 1296929 |
| profiling of propionibacterium acnes recovered from root canal and blood during and after endodontic treatment. | this report describes the first results of an ongoing study of bacteremia after endodontic treatment of teeth with asymptomatic apical periodontitis. after access cavity preparation, microbiological samples were taken from the root canal under aseptic conditions in 4 single-rooted teeth in 4 patients. in treatment of 2 of the patients, the first 3 reamers (sizes 15-25) were deliberately used to a level 2 mm beyond the apical foramen. in 2 patients the instrumentation ended inside the root canal ... | 1992 | 1302689 |
| adjuvant properties of propionibacterium avidum kp-40 in vaccination against endemic viral and bacterial infections. ii. swine immunized with inactivated haemophilus pleuropneumoniae vaccine and experimentally infected with different virulent serotypes of h. pleuropneumoniae. | forty 3-month old swine were treated with immunomodulating propionibacterium avidum kp-40 (pa) and/or vaccinated with a formalin-inactivated mixture of serotypes 1, 3, 5 and 9 of haemophilus pleuropneumoniae (pleurovac). three weeks after revaccination all animals were inoculated with viable single serotypes of haemophilus pleuropneumoniae. the igg antibodies induced by vaccination agglutinated all serotypes of haemophilus pleuropneumoniae, except for serotype 5. antibody titers were not influen ... | 1992 | 1303696 |
| adjuvant properties of propionibacterium avidum kp-40 in vaccination against endemic viral and bacterial infections. iii. swine immunized with live attenuated erysipelothrix rhusiopathiae vaccine and experimentally infected with virulent strains r203 and r270b of e. rhusiopathiae. | fifty 4-month old piglets were treated with immunomodulating propionibacterium avidum kp-40 (pa) and/or immunized with live attenuated erysipelothrix rhusiopathiae vaccine (orvac). four weeks after vaccination all animals were inoculated with viable erysipelothrix rhusipathiae. the vaccine induced the appearance of high titers of specific igg antibodies with peak values (1:115-1:200) three weeks after immunization. administration of pa together with the vaccine did not influence antibody titers. ... | 1992 | 1303697 |
| [the efficacy of a combined therapy against herpes virus genital infections]. | a complex immuno-chemical therapy (inhibition of viral replication, stimulation of cell mediated immunity, interferon induction) was applied to 75 patients with herpes virus genital infections. treatment was followed by complete recovery in 66.7% of the subjects and by considerable reduction of relapse frequency and of pain in the others. | 1992 | 1308432 |
| susceptibility of anaerobic bacteria to pd 131628. | the in vitro activity of pd 131628 against anaerobic cocci, propionibacterium acnes, clostridium perfringens, clostridium difficile, bacteroides fragilis, bacteroides spp. and fusobacteria was determined by the agar dilution method. this activity was compared with that of ciprofloxacin, piperacillin, cefoxitin, imipenem, clindamycin, metronidazole and chloramphenicol. pd 131628, imipenem, clindamycin, metronidazole and chloramphenicol were the most active agents tested. | 1992 | 1314176 |
| association between synthesis and release of cgmp and nitric oxide biosynthesis by hepatocytes. | hepatocytes are known to synthesize nitric oxide (no) from l-arginine via an inducible no synthase. studies were performed to determine the relationship between hepatocyte no production and the stimulation of hepatocyte soluble guanylate cyclase. a combination of lipopolysaccharide (lps), interferon-gamma, tumor necrosis factor, and interleukin-1 stimulates the biosynthesis of large quantities of nitrite and nitrate (no2- + no3-). hepatocyte no2- + no3- production was associated with only small ... | 1992 | 1314486 |
| effect of 1,1-dimethylhydrazine (udmh) on corynebacterium parvum-associated immunosuppression in mice. | these studies further investigate the immunoenhancement properties of udmh by utilizing corynebacterium parvum-induced immunosuppressed mice as well as evaluating activated macrophage production of reactive oxygen intermediates or their effects. forty-eight hour con a-induced lymphoblastogenic responses from splenocytes isolated from c. parvum and udmh-treated balb/c mice were significantly increased compared with c. parvum alone, although less than normal control mice (no treatment). in vitro b ... | 1992 | 1316318 |
| identification of critical lysyl residues in the pyrophosphate-dependent phosphofructo-1-kinase of propionibacterium freudenreichii. | pyrophosphate-dependent 6-phosphofructo-1-kinase (ppi-pfk) from propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (plp) after sodium borohydride reduction. the substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactivation whereas inorganic pyrophosphate had little effect. an hplc profile of a tryptic digest of ppi-pfk modified at low concentrations of plp showed a single major peak with only a ... | 1992 | 1317210 |
| increased 5-lipoxygenase activity in massive hepatic cell necrosis in the rat correlates with neutrophil infiltration. | rats were treated with heat-killed propionibacterium acnes and subsequent injection of a small amount of lipopolysaccharide after 7 days. after 24 hr most of the rats died of massive liver cell necrosis. nonparenchymal liver cells were isolated from this liver injury model and incubated with arachidonic acid. reverse-phase high-pressure liquid chromatography detected the 5-lipoxygenase metabolites (leukotriene b4 and 5-hydroxy-arachidonic acid), whereas these compounds were produced in negligibl ... | 1992 | 1322350 |
| invasion of streptococcus mutans, streptococcus intermedius and propionibacterium acnes into the teeth of gnotobiotic rats. | germ-free rats, fed a modified cariogenic diet 2000 with a reduced sucrose content of 25%, were inoculated with a strain of streptococcus mutans, streptococcus intermedius or propionibacterium acnes, respectively. after 100 days on the diet, the rats were sacrificed. microscopic examination of stained decalcified tooth sections revealed marked destruction of the outer zones of the dentin infected with s. mutans. the destruction was closely associated with the formation of glucan-mediated large a ... | 1992 | 1325873 |
| inhibition of nitric oxide synthesis during endotoxemia promotes intrahepatic thrombosis and an oxygen radical-mediated hepatic injury. | corynebacterium parvum-treated mice produce large amounts of circulating nitrogen oxides and develop a severe liver injury in response to lipopolysaccharide (lps). concurrent administration of ng-monomethyl-l-arginine not only suppresses nitric oxide synthesis in these animals but also profoundly increases the hepatic damage following lps. in this report, we present evidence that the increased hepatic damage from inhibition of nitric oxide synthesis is mediated in part by superoxide and hydroxyl ... | 1992 | 1328440 |
| comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms. | the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ... | 1992 | 1331019 |
| inhibitory effect of bis(2-aminohexyl) disulfide and bis(2-amino-3-phenylpropyl) disulfide on several mouse inflammations. | the anti-inflammatory profile of the analogues of bis(2-aminopropyl) disulfide dihydrochloride with butyl (compd. ii) and phenyl (compd. iii) instead of the methyl group was studied in several mouse models related to phagocyte functions. the test samples were administered 2-3 h before the inflammatory stimulation or the peak of inflammation. subcutaneously administered, compds. ii and iii significantly inhibited serotonin-induced paw edema in a dose-dependent manner (50% inhibitory dose values: ... | 1992 | 1335844 |
| adjuvant properties of propionibacterium avidum kp-40 in vaccination against endemic viral and bacterial infections. i. swine immunized with live attenuated aujeszky's disease virus vaccine and experimentally infected with virulent viruses. | forty 5-month old swine were treated with immunomodulating propionibacterium avidum kp-40 (pa) and/or immunized with live attenuated aujeszky's disease (ad) virus vaccine (suivaca); 8 weeks later all animals were infected with virulent ad viruses (nia-3). seven of 10 swine vaccinated without pa developed mild/moderate symptoms of infection with 3- to 5-day fever and a temporary halt in weight gain. application of pa together with the vaccine lowered the morbidity rate, shortened the period of fe ... | 1992 | 1338942 |
| [recurrent herpetic gingivostomatitis treated with moroxydine, antiherpin and the immunostimulant sre]. | | 1992 | 1340264 |
| experimental nonspecific immunostimulation by the propionibacterium acnes vaccine. | the immunostimulation effect of the selected propionibacterium acnes strain 16073 was described. the optimal conditions for the preparation of the p. acnes vaccine and the nonspecific immunostimulation effects of the vaccine on various experimental infections were evaluated. | 1992 | 1344592 |
| alpha-d-galactosylation of surface fucoglycoconjugate(s) upon stimulation/activation of murine peritoneal macrophages. | murine resident macrophages express, on their surface, carbohydrate epitopes which undergo changes during their stimulation/activation as monitored by binding of 125i labelled evonymus europaea and griffonia simplicifolia i-b4 lectins. treatment of the stimulated macrophages with coffee bean alpha-galactosidase abolished binding of the gs i-b4 isolectin and changed the binding pattern of the evonymus lectin. the affinity (ka) of evonymus lectin for alpha-galactosidase-treated macrophages decreas ... | 1992 | 1344714 |
| immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium. | immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ... | 1992 | 1354674 |
| lymphocyte proliferation and t-lymphocyte subsets during experimental immunomodulation of balb/c mice by olimunostim. | to objectivize possible immunomodulatory effects of polybacterial lysate olimunostim (p. acnes, k. pneumoniae, s. aureus), splenic lymphocyte proliferation and t-lymphocyte subsets were followed-up in balb/c mice administered perorally the lysate or saline (controls). the enhanced spontaneous lymphocyte proliferation observed at the beginning of olimunostim application preceded a gradual increase of lymphocyte reactivity to t-mitogens reaching the maximum five days after the administration of th ... | 1992 | 1364967 |
| study of modulatory effects of olimunostim on cellular immunity in healthy humans. | to objectivize potential modulatory effects of polybacterial lysate olimunostim on cellular immunity, the t-lymphocyte subpopulations and lymphocyte reactivity to mitogens were followed up in 10 healthy volunteers subjected to olimunostim application and parallelly in 6 healthy individuals receiving placebo. the preparation, which was applied perorally in seven consecutive daily doses, had no effects on t-lymphocytes and their subpopulations and exerted only a limited influence on lymphocyte rea ... | 1992 | 1364968 |
| in vivo biotinylation of fusion proteins expressed in escherichia coli with a sequence of propionibacterium freudenreichii transcarboxylase 1.3s biotin subunit. | biotinylation of fusion proteins in e. coli was studied using a sequence of propionibacterium freudenreichii transcarboxylase 1.3s biotin subunit. as the biotinylation sequence, we examined two sequences: one was of amino acid residues [84-123] of 1.3s, a partial sequence containing a region from a conserved tetrapeptide (ala-met-bct-met) around the biotinyl lysine (bct) to the carboxyl terminal; the other was of an almost entire sequence [18-123]. we constructed recombinant plasmids for fusion ... | 1992 | 1368826 |
| induction of ca2+/calmodulin-dependent no synthase in various organs of rats by propionibacterium acnes and lipopolysaccharide treatment. | ca2+/calmodulin-dependent nitric oxide synthase was found to be induced during rat liver necrosis caused by administration of propionibacterium acnes and e. coli lipopolysaccharide to rats. examination of the specific induction of ca2+/calmodulin-dependent no synthase showed that the enzyme was induced in the lung, spleen and colon as well as the liver. northern blot analysis revealed that the induction occurred at the transcriptional level. | 1992 | 1379549 |
| polyclonal antibody against an inducible form of nitric oxide synthase purified from the liver of rats treated with propionibacterium acnes and lipopolysaccharide. | a polyclonal antibody was raised in the rabbit against an inducible form of nitric oxide (no) synthase (ec 1.14.23) purified from the liver of rats with acute liver necrosis induced by i.v. administration of propionibacterium acnes and lipopolysaccharide. the antibody immunoprecipitated no synthase activities in the soluble extract of the liver from treated rats. western blot analysis showed that the cytosols of the liver, lung and spleen from the treated rats but not from non-treated rats, and ... | 1992 | 1384469 |
| inhibition of neutrophil chemotactic factor production in comedonal bacteria by subminimal inhibitory concentrations of erythromycin. | the effect of 1/10 minimal inhibitory concentrations (sub-mic) of erythromycin (em) on human neutrophil chemotactic factor production in comedonal bacteria, propionibacterium acnes strains, propionibacterium granulosum strains and coagulase-negative staphylococcus (cns) strains was assayed using the boyden chamber method. sub-mic of em significantly suppressed neutrophil chemotactic factor production in all strains of p. acnes, p. granulosum and cns. our results suggest that sub-mic of em may ha ... | 1992 | 1386264 |
| propionibacterium acnes causing an aortic root abscess. | a case of endocarditis caused by propionibacterium acnes associated with an aortic root abscess is presented. this supports the current opinion that aortic root abscesses are not necessarily associated with microorganisms of high virulence. | 1992 | 1389743 |
| a case of propionibacterium acnes spinal osteomyelitis. | | 1992 | 1396737 |
| bacterial invasion of non-exposed dental pulp. | anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ... | 1992 | 1399049 |
| anaerobic bacterial infections causing osteomyelitis/arthritis in a dog. | a 3-year-old german shepherd dog was examined for lameness, signs of pain, swelling, a draining fistulous tract, and osteolysis after a dog bite on the left carpus. after failure of the lesion to respond to several antibiotics, peptostreptococcus sp and propionibacterium sp were isolated from swab specimens and then from surgically collected bone and soft tissue specimens. the bone fragments had mild purulent osteomyelitis associated with numerous gram-positive rods and cocci. the dog was succes ... | 1992 | 1399798 |
| [bacteriology of infectious disease in otorhinolaryngology (1). bacteriological study of paranasal cyst]. | the bacteriology of chronic sinusitis has been reported in several studies, but there have been few bacteriological studies on paranasal cyst. also, details of the techniques of transportation and processing of clinical specimens have only rarely been described. therefore, this study was undertaken with attention to the above, and to obtain information on appropriate antimicrobial therapy, we conducted a dilution antimicrobial susceptibility test of isolates. fifteen patients with paranasal cyst ... | 1992 | 1403316 |
| [hair preparation caused fever and clouding of consciousness]. | | 1992 | 1405847 |
| pathogenicity of actinomyces israelii and arachnia propionica: experimental infection in guinea pigs and phagocytosis and intracellular killing by human polymorphonuclear leukocytes in vitro. | strains of actinomyces israelii and arachnia propionica, isolated from clinical cases of failed endodontic therapy, were examined for: (i) their ability to survive and establish themselves in the soft connective tissue that grew into subcutaneously implanted tissue cages in guinea pigs; (ii) cell-surface hydrophobicity; and (iii) phagocytosis and killing by human polymorphonuclear leukocytes (pmns) under aerobic and anaerobic conditions. bacteria were inoculated into the tissue cages in guinea p ... | 1992 | 1408347 |
| [tibial hyperostosis and propionibacterium acnes]. | in a 39-year-old patient with tibial productive osteitis, propionibacterium acnes was identified in a surgical specimen of the bone lesion. similar cases have been reported by others. the possibility that p. acnes may play in a pathogenic role in the sapho syndrome by causing inflammatory or infectious changes is discussed. | 1992 | 1411197 |
| systemic il-1 and adjuvant treatment of an experimental tumor. i. immune status following tumor rechallenge. | local adjuvant therapy of weakly immunogenic tumors protects against primary tumor challenge. however, this form of therapy does not produce long-lasting immunity to the tumor. in this study, local adjuvant therapy combined with systemic il-1 administration produced not only primary tumor protection, but also long lasting immunity to the tumor. il-1 and adjuvant protected animals resisted rechallenge with tumor as much as 180 days after initial tumor administration. resistance to tumor rechallen ... | 1992 | 1419470 |
| in vitro activity of l-627 against anaerobic bacteria. | the in vitro activity of l-627 against 370 anaerobic bacterial strains including anaerobic cocci, propionibacterium acnes, clostridium perfringens, clostridium difficile, bacteroides fragilis, other bacteroides spp. and fusobacteria was determined by the agar dilution method. this activity was compared with that of piperacillin, cefoxitin, imipenem, meropenem, clindamycin, metronidazole and chloramphenicol. l-627, imipenem, meropenem, clindamycin, metronidazole and chloramphenicol were the most ... | 1992 | 1425740 |
| immunotherapy of chronic active viral hepatitis b with propionibacterium granulosum kp-45 (a 5-year follow-up report). | in the period 1982 to 1984, 14 hbs-ag-positive patients with chronic active hepatitis b were treated monthly with a cell wall preparation (5 or 10 mg) of propionibacterium granulosum kp-45 (pg), intravenously administered for a period of 6 to 10 months. all 14 patients were monitored by serological and biochemical tests as well as liver biopsy two, three and five years after completing immunotherapy with pg. during this period the patients received neither a specific antiviral, corticosteroid or ... | 1992 | 1427577 |
| comparisons of the bacterial flora in genital regions at non-pregnancy. | in the development of infectious diseases at non-pregnancy and at pregnancy, correlations between bacterial flora in the vagina and portio vaginalis and the ascending infections of those bacteria have recently been discussed. to clarify the cause of those infectious diseases, we studied the localization of microorganisms in genital regions. patients undergone abdominal total hysterectomy (n = 172) were employed as subjects, and microorganisms isolated from 4 genital regions were studied. in addi ... | 1992 | 1433896 |
| inhibitory effect of (4r)-hexahydro-7,7-dimethyl-6-oxo-1,2,5-dithiazocine-4-carboxylic acid (sa3443), a novel cyclic disulfide, on the production of tnf-like factor from propionibacterium acnes-primed rat liver macrophages/kupffer cells. | the effect of (4r)-hexahydro-7,7-dimethyl-6-oxo-1,2,5-dithiazocine-4-carboxylic acid (sa3443), a novel cyclic disulfide, on tumor necrosis factor (tnf)-like factor production from propionibacterium acnes (p. acnes)-primed rat liver macrophages/kupffer cells was investigated. a remarkable increase in tnf-like activity was detected in the culture supernatants of the liver macrophages/kupffer cells from p. acnes-treated rats. at concentrations of 1 x 10(-6) to 1 x 10(-4) m, sa3443 significantly inh ... | 1992 | 1434146 |
| [propionibacterium acnes in the etiology of endocarditis]. | propionibacterium acnes is the gram positive anaerobic bacteria belongs to the normal skin and oral microbial flora. the participation of this microorganism in the infective endocarditis is still controversial. the aim of the study was to perform the diagnostic and therapeutic difficulties in 5 patients with infective endocarditis caused by propionibacterium acnes. in 3 out of 5 patients the infective endocarditis developed after prosthesis valve replacement, in 2 others on the native valves. th ... | 1992 | 1437777 |
| beneficial effects of sk&f 105809, a novel cytokine-suppressive agent, in murine models of endotoxin shock. | sk&f 105809 is a structurally novel dual inhibitor of lipoxygenase and cyclooxygenase-mediated arachidonic acid (aa) metabolism, which has demonstrated antiinflammatory activity in rodent models of inflammation. in addition, the active metabolite of this compound, sk&f 105561, has been shown in vitro to inhibit the production of the inflammatory cytokine interleukin-1 (il-1) in human monocytes stimulated with lipopolysaccharide (lps). we report here that in vitro sk&f 105561 also blocks the prod ... | 1992 | 1446388 |
| the arginine-dependent cytolytic mechanism plays a role in destruction of naegleria fowleri amoebae by activated macrophages. | mouse peritoneal macrophages activated by different immunomodulators (mycobacterium bovis bacillus calmette-guérin or propionibacterium acnes) destroy naegleria fowleri amoebae by a contact-dependent process and by soluble cytolytic molecules secreted by macrophages in response to lipopolysaccharide. the goal of this study was to determine whether the arginine-dependent cytolytic mechanism which results in the production of nitric oxide from arginine by activated macrophages destroys the amoebae ... | 1992 | 1452346 |
| antibacterial activity of totarol and its potentiation. | antimicrobial activity of six diterpenoids isolated from the bark of podocarpus nagi (podocarpaceae) has been tested against twelve selected microorganisms. totarol [1], the most abundant compound among the six, exhibited potent bactericidal activity only against gram-positive bacteria, among which propionibacterium acnes was the most sensitive bacterium. totarol also showed strong activity against four other gram-positive bacteria tested: streptococcus mutans, bacillus subtilis, brevibacterium ... | 1992 | 1453180 |
| effects of povidone-iodine chemical preparation and saline irrigation on the perilimbal flora. | to analyze the effects of 5% povidone-iodine preparation and saline irrigation on the species composition of perilimbal flora. | 1992 | 1454324 |
| prevention of 5-fluorouracil-induced infection with indigenous escherichia coli in tumor-bearing mice by nonspecific immunostimulation. | we have previously reported that the lethal toxicity of 5-fluorouracil (5-fu) in specific-pathogen-free mice is due to an indigenous infection with escherichia coli (k. nomoto, t. yokokura, y. yoshikai, et al. can. j. microbiol. 37:244-247, 1991). in the present study, we demonstrate that nonspecific immunostimulation augments host resistance against the lethal toxicity of 5-fu in tumor-bearing mice. intravenous administration of a preparation of heat-killed lactobacillus casei (lc 9018), a nons ... | 1992 | 1458370 |
| the incidence of bacteraemia following laryngeal mask insertion. | the incidence of bacteraemia following insertion of the laryngeal mask airway (lma) was investigated in one hundred fit patients. four cultures were positive: three represented contamination with skin flora; the other was a microaerophilic streptococcus grown from an anaerobic culture bottle. although this organism can be pathogenic, it may also represent contamination. our findings suggest that significant bacteraemia on insertion of the lma is uncommon and is probably no more than with oral in ... | 1992 | 1463178 |
| 1,25-dihydroxyvitamin d3 inhibits thromboxane release from activated macrophages. | 1,25-dihydroxyvitamin d3 (1,25-(oh)2d3) in concentrations from 0.1 to 10 nm suppressed immunoreactive thromboxane b2 (itxb2) release from propionibacterium acnes (p. acnes)-elicited liver adherent cells stimulated with lipopolysaccharide (lps, 1 microgram/ml). these suppressive effects of 1,25-(oh)2d3 were also observed in oyster glycogen-elicited peritoneal macrophages. on the contrary, it did not inhibit itxb2 release from both resident kupffer cells and peritoneal macrophages stimulated with ... | 1992 | 1475528 |
| sterility of refrigerated injectable collagen syringes after injection of patient. | injectable collagen has been used for more than 15 years to correct soft tissue cosmetic defects. after injection, the collagen remaining in the syringe is often refrigerated for later use in the same patient, despite manufacturer and food and drug administration recommendations to discard the unused collagen. | 1992 | 1479101 |
| propionibacterium endophthalmitis. | | 1992 | 1480388 |
| importance of direct hepatocytolysis by liver macrophages in experimental fulminant hepatitis. | when lipopolysaccharide was administered to mice that had been injected with heat-killed propionibacterium acnes, most of them died of massive liver necrosis. previously, we demonstrated that a soluble hepatocytotoxic factor released by liver adherent cells fully activated by both p. acnes and lipopolysaccharide was attributable to the late stage of this severe liver injury. in this report, we focused on the hepatocytolysis by these liver adherent cells in a cell-cell interaction manner. shortly ... | 1992 | 1483670 |
| culture media for non-sporulating gram-positive food spoilage bacteria. | the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ... | 1992 | 1486021 |
| propionibacterium acnes-metabolites inhibit experimental lung metastasis of murine sarcoma l-1 in balb/c-mice. | adhesive interactions between tumor cells and host tissue occur at several stages of metastasis. such interactions might be inhibited by microbial metabolites resembling the binding regions of matrix molecules. certain metabolite sequences including gly, asp, arg, and ser (gaas) proved to be critical for cell interactions, e.g. with fibronectin. in vitro, the rosette formation of murine pulmonary cells and sarcoma l-1 cells decreased significantly in the presence of propionibacterium acnes-metab ... | 1992 | 1486236 |
| comparative activity of the topical quinolone opc-7251 against bacteria associated with acne vulgaris. | the antibacterial activity of the topical quinolone opc-7251 against bacteria commonly found in acne vulgaris was tested in vitro by an agar dilution method. the mic50 was 0.25 mg/l for propionibacterium acnes, 0.125 mg/l for propionibacterium granulosum, 0.03 mg/l for staphylococcus aureus and 0.06 mg/l for coagulase-negative staphylococci. compared with seven other antibiotics tested (ciprofloxacin, penicillin, erythromycin, tetracycline, clindamycin, fusidic acid and gentamicin), opc-7251 had ... | 1992 | 1486893 |
| study on the mechanism of an experimental immunological intrahepatic cholestasis model. | tuberculin-sensitized guinea pigs were intravenously injected with heat-killed propionibacterium acnes followed by an intravenous injection of purified protein derivatives 7 day later, resulting in the induction of intrahepatic cholestasis. using this experimental model, the following results were obtained: (1) both uptake and release of bile acid were inhibited in the hepatocytes prepared from the cholestasis guinea pigs. (2) the results of the erythritol clearance method indicated that the dec ... | 1992 | 1488254 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| preliminary development of a live drug-controlled vaccine against bovine babesiosis using the mongolian gerbil, meriones unguiculatus. | this study investigated the practicality and potential of the gerbil, meriones unguiculatus, as a source of live babesia divergens vaccine and also as a model for the use of the vaccine in cattle. a series of experiments with gerbils concerning vaccine infectivity, immunogenicity and safety were carried out. it was concluded that the use of rpmi medium/40% foetal calf serum as a diluent improved vaccine infectivity, but that the parasitaemia of the blood obtained from donor gerbils had little or ... | 1992 | 1496778 |
| chronic postoperative endophthalmitis caused by propionibacterium acnes. | persistent intraocular inflammation after cataract surgery with intraocular lens implantation is acquiring importance. frequently, chronic uveitis or the "toxic lens syndrome" have to be differentiated from bacterial infection. this report describes five cases with chronic postoperative endophthalmitis where the anaerobic bacterium propionibacterium acnes was found to be the causative organism. adequate anaerobic culture media and proper sampling from the area around the lens haptics are the mos ... | 1992 | 1498574 |
| biosynthesis of vitamin b12. transformation of riboflavin 2h-labeled in the 1'r position of 1's position into 5,6-dimethylbenzimidazole. | the 5,6-dimethylbenzimidazole moiety of vitamin b12 is formed from riboflavin in aerobic and some aerotolerant bacteria. thereby c1' of riboflavin is transformed into c2 of the vitamin b12 base. in the present publication a study on this transformation with riboflavin 2h-labeled in the 1'r or 1's position is described. this study was undertaken in order to find out if one of the two hydrogens at c1' is transferred to c2 of 5,6-dimethylbenzimidazole. the 2h-labeled riboflavin samples were synthes ... | 1992 | 1499570 |
| enhancement of prostaglandin e2 production by liver macrophages (kupffer cells) after stimulation with biological response modifiers. | pge2 production by liver macrophages (kupffer cells) activated by biological response modifiers was examined. kupffer cells obtained from a normal rat liver possessed cyclooxygenase activity and produced txb2, pgd2, and pge2 from (1-14c)arachidonic acid. the major product was pgd2. when kupffer cells were incubated in the presence of lipo-polysaccharide (lps), ok-432, or heat-killed propionibacterium acnes for 24 h, the amount of arachidonate cyclooxygenase products increased and the major produ ... | 1992 | 1502248 |