| relationships between the use of embden meyerhof pathway (emp) or phosphoketolase pathway (pkp) and lactate production capabilities of diverse lactobacillus reuteri strains. | the aims of this study is to compare the growth and glucose metabolism of three lactobacillus reuteri strains (i.e. dsm 20016, dsm 17938, and atcc 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). however, the physiological diversity inside the species, even for basic metabolisms, like its capacity o ... | 2015 | 26428921 |
| properties of lactobacillus reuteri chitosan-calcium-alginate encapsulation under simulated gastrointestinal conditions. | the protective effects of encapsulation on the survival of lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. viable counts and the remaining probiotic properties of calcium (ca)-alginate encapsulated (a group), chitosan-ca-alginate encapsulated (ca group), and unencapsulated, free l. reuteri (f group) were determined. encapsulation improved the survival of l. reuteri subjected to simulated gastrointestin ... | 2015 | 26415668 |
| diversity of lactobacillus reuteri strains in converting glycerol into 3-hydroxypropionic acid. | the present study aims at comparing the performances of three lactobacillus reuteri strains (dsm 20016, dsm 17938, and atcc 53608) in producing 3-hydroxypropionic acid (3-hp) from glycerol and at exploring inhibition phenomena during this bioconversion. differences were highlighted between the three strains in terms of 3-hp production yield, kinetics of substrate consumption, and metabolite production. with a maximal productivity in non-optimal conditions (free ph) around 2 g.l(-1).h(-1) of 3-hp ... | 2015 | 26319567 |
| a study on enhanced expression of 3-hydroxypropionic
acid pathway genes and impact on its production in lactobacillus reuteri. | 3-hydroxypropionic acid (3-hp) is a novel antimicrobial agent against foodborne pathogens like salmonella and staphylococcus species. lactobacillus reuteri converts glycerol into 3-hp using a coenzyme a-dependent pathway, which is encoded by propanediol utilization operon (pdu) subjected to catabolite repression. in a catabolite-repression-deregulated l. reuteri rprb3007, quantitative pcr revealed a 2.5-fold increase in the transcripts of the genes pdup, pduw and pdul during the mid-log phase of ... | 2015 | 27904365 |
| encapsulation and subsequent freeze-drying of lactobacillus reuteri crl 1324 for its potential inclusion in vaginal probiotic formulations. | probiotic formulations must include a high number of viable and active microorganisms. in this work, the survival of human vaginal lactobacillus reuteri crl 1324 during encapsulation, lyophilization and storage, and the activity of encapsulated and/or freeze-dried bacterial cells were evaluated. extrusion-ionic gelation technique was applied to encapsulate l. reuteri crl 1324, using xanthan and gellan. encapsulated and free bacterial cells were freeze-dried with or without lactose and skim milk ... | 2015 | 26299342 |
| synthesis of oligo- and polysaccharides by lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose. | gtfa, a glucansucrase enzyme of the probiotic bacterium lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1 → 4) and (1 → 6) linkages from sucrose. with respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. it appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharid ... | 2015 | 26281004 |
| physicochemical properties of a water soluble extracellular homopolysaccharide from lactobacillus reuteri sk24.003. | a water soluble exopolysaccharide was obtained from lactobacillus reuteri sk24.003 and its rheological and structural properties were investigated. the polysaccharide yield reached around 40.8g/l for 48h of fermentation. the purified exopolysaccharide was an α-d-glucan with the molecular weight of 2.50×10(7)g/mol. thermal analysis showed the exopolysaccharide broke down above 200°c, the weight dramatically lost (around 53.0%) from 230 to 340°c and the melting point was 147.7°c with the enthalpy ... | 2015 | 26256197 |
| use of phytone peptone to optimize growth and cell density of lactobacillus reuteri. | the objective of this study was to determine the use of phytone peptone to optimize the growth and cell density of lactobacillus reuteri. four strains of l. reuteri (dsm 20016, sd 2112, cf 2-7f, and mf 2-3,) were used in this study. an overnight culture of individual strains was inoculated into fresh basal media with various protein sources (peptone, tryptone, proteose peptone #3, phytone peptone, tryptic soy broth, yeast extract, and beef extract). samples were then mixed well and incubated at ... | 2015 | 28231207 |
| supplementary heat-killed lactobacillus reuteri gmnl-263 ameliorates hyperlipidaemic and cardiac apoptosis in high-fat diet-fed hamsters to maintain cardiovascular function. | obesity and hyperlipidaemia increase the risk of cvd. some strains of probiotics have been suggested to have potential applications in cardiovascular health by lowering serum ldl-cholesterol. in this work, high-fat diet-induced hyperlipidaemia in hamsters was treated with different doses (5×108 and 2·5×109 cells/kg per d) of heat-killed lactobacillus reuteri gmnl-263 (lr263) by oral gavage for 8 weeks. the serum lipid profile analysis showed that ldl-cholesterol and plasma malondialdehyde (p-mda ... | 2015 | 26234728 |
| bio-transformation of glycerol to 3-hydroxypropionic acid using resting cells of lactobacillus reuteri. | lactobacillus reuteri grown in mrs broth containing 20 mm glycerol exhibits 3.7-fold up-regulation of 3-hydroxypropionic acid (3-hp) pathway genes during the stationary phase. concomitantly, the resting cells prepared from stationary phase show enhancement in bio-conversion of glycerol, and the maximum specific productivity (q p) is found to be 0.17 g 3-hp per g cdw per hour. the regulatory elements such as catabolite repression site in the up-stream of 3-hp pathway genes are presumed for the au ... | 2015 | 26204968 |
| effect of probiotic bacteria on oral candida in frail elderly. | the aim of this study was to investigate the effect of a daily intake of probiotic lactobacilli on the prevalence and counts of oral candida in frail elderly patients living in nursing homes. the study had a double-blind randomized placebo-controlled design with 2 parallel arms. the study group consisted of 215 older adults (range, 60 to 102 y) who were enrolled after informed consent. after baseline examination and randomization, the subjects were given 1 lozenge containing 2 strains of the pro ... | 2015 | 26202995 |
| early colonization of lactobacillus reuteri after exposure to probiotics. | the aim of the present in vivo animal study is to investigate the ability of l. reuteri to colonize the oral flora during infancy. | 2015 | 26161603 |
| probiotic lactobacillus rhamnosus gg therapy and microbiological programming in infantile colic: a randomized, controlled trial. | probiotic lactobacillus reuteri and reduced allergen load may lessen the daily crying of colic infants, but the role of lactobacillus rhamnosus gg (lgg) has remained obscure. | 2015 | 26151493 |
| loss of bone and wnt10b expression in male type 1 diabetic mice is blocked by the probiotic lactobacillus reuteri. | type 1 diabetes (t1d)-induced osteoporosis is characterized by a predominant suppression of osteoblast number and activity, as well as increased bone marrow adiposity but no change in osteoclast activity. the fundamental mechanisms and alternative anabolic treatments (with few side effects) for t1d bone loss remain undetermined. recent studies by our laboratory and others indicate that probiotics can benefit bone health. here, we demonstrate that lactobacillus reuteri, a probiotic with anti-infl ... | 2015 | 26135835 |
| use of anaerobic green fluorescent protein versus green fluorescent protein as reporter in lactic acid bacteria. | lactic acid bacteria (lab) are commonly used in the production of fermented and probiotic foods. development of molecular tools to discriminate the strains of interest from the endogenous microbiota in complex environments like food or gut is of high interest. green fluorescent protein (gfp)-like chromophores strictly requires molecular oxygen for maturation of fluorescence, which restrict the study of microorganisms in low-oxygen environments. in this work, we have developed a noninvasive cyan- ... | 2015 | 26129953 |
| [efficacy of racecadotril vs. smectite, probiotics or zinc as an integral part of treatment of acute diarrhea in children under five years: a meta-analysis of multiple treatments]. | despite major advances in treatment, acute diarrhea continues to be a public health problem in children under five years. there is no systematic approach to treatment and most evidence is assembled comparing active treatment vs. placebo. | 2015 | 26089269 |
| efficient mannitol production by wild-type lactobacillus reuteri crl 1101 is attained at constant ph using a simplified culture medium. | mannitol is a natural polyol with multiple industrial applications. in this work, mannitol production by lactobacillus reuteri crl 1101 was studied at free- and controlled-ph (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. the activity of mannitol 2-dehydrogenase (mdh), the enzyme responsible for mannitol synthesis, was determined. the effect of the initial biomass concentration was further studied. mannitol production (41.5 ± ... | 2015 | 26084891 |
| the trpv1 channel in rodents is a major target for antinociceptive effect of the probiotic lactobacillus reuteri dsm 17938. | certain probiotic bacteria have been shown to reduce distension-dependent gut pain, but the mechanisms involved remain obscure. live luminal lactobacillus reuteri (dsm 17938) and its conditioned medium dose dependently reduced jejunal spinal nerve firing evoked by distension or capsaicin, and 80% of this response was blocked by a specific trpv1 channel antagonist or in trpv1 knockout mice. the specificity of dsm action on trpv1 was further confirmed by its inhibition of capsaicin-induced intrace ... | 2015 | 26084409 |
| intake of lactobacillus reuteri improves incretin and insulin secretion in glucose-tolerant humans: a proof of concept. | ingestion of probiotics can modify gut microbiota and alter insulin resistance and diabetes development in rodents. we hypothesized that daily intake of lactobacillus reuteri increases insulin sensitivity by changing cytokine release and insulin secretion via modulation of the release of glucagon-like peptides (glp)-1 and -2. | 2015 | 26084343 |
| lactobacillus reuteri dsm 17938 as a probiotic for preterm neonates: a strain-specific systematic review. | prevention of necrotizing enterocolitis (nec) while optimizing enteral nutrition (en) is a priority in preterm neonates. lactobacillus reuteri dsm 17938 (l reuteri) is known to improve gut motility. previous systematic reviews have not adequately assessed the effects of l reuteri in improving feed tolerance in preterm neonates. | 2016 | 26059900 |
| impact of a yogurt matrix and cell microencapsulation on the survival of lactobacillus reuteri in three in vitro gastric digestion procedures. | the goal of this study was to assess the interaction between microencapsulation and a yogurt food matrix on the survival of lactobacillus reuteri ncimb 30242 in four different in vitro systems that simulate a gastric environment. the four systems were: united states pharmacopeia (usp) solutions, a static two-step (sts) procedure which included simulated food ingredients, a constantly dynamic digestion procedure (ividis), as well a multi-step dynamic digestion scheme (s'ividis). the ph profiles o ... | 2015 | 26036146 |
| hypolipidemic effects and safety of lactobacillus reuteri 263 in a hamster model of hyperlipidemia. | we aimed to verify the beneficial effects of probiotic strain lactobacillus reuteri 263 (lr263) on hypolipidemic action in hamsters with hyperlipidemia induced by a 0.2% cholesterol and 10% lard diet (i.e., high-cholesterol diet (hcd)). male golden syrian hamsters were randomly divided into two groups: normal (n = 8), standard diet (control), and experimental (n = 32), a hcd. after a two-week induction followed by a six-week supplementation with lr263, the 32 hyperlipidemic hamsters were divided ... | 2015 | 25988768 |
| lactobacillus reuteri dsm 17938 shortens acute infectious diarrhea in a pediatric outpatient setting. | two randomized controlled clinical trials have shown that lactobacillus (l) reuteri dsm 17938 reduces the duration of diarrhea in children hospitalized due to acute infectious diarrhea. this was the first trial evaluating the efficacy of l. reuteri dsm 17938 in outpatient children with acute infectious diarrhea. | 2015 | 25986615 |
| divergent signaling pathways regulate il-12 production induced by different species of lactobacilli in human dendritic cells. | recent studies have indicated that different strains of lactobacilli differ in their ability to regulate il-12 production by dendritic cells (dcs), as some strains are stronger inducer of il-12 while other are not and can even inhibit il-12 production stimulated by il-12-inducer lactobacilli. in this report we demonstrate that lactobacillus reuteri 5289, as previously described for other strains of l. reuteri, can inhibit dc production of il-12 induced by lactobacilllus acidophilus ncfm. remarka ... | 2015 | 25977118 |
| analysis of biofilm formation by intestinal lactobacilli. | in this study, the biofilm-forming potential of intestinal lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. moreover, the spatial organization of exogenously applied l. reuteri l2/6 (a pig isolate) at specific locations in gastrointestinal tract of monoassociated mice was investigated by fluorescence in situ hybridization. we did not detect biofilm formation by tested strains in nutrient-rich de man-rogosa-sharpe (mrs) medium. ... | 2015 | 25961850 |
| enhancing vitamin b12 content in soy-yogurt by lactobacillus reuteri. | more attention from the aged and vegetarians has been paid to soy-product due to its taste, easy digestibility, as well as the association with health. however, soy-product has a defect of low vitamin content, mainly the water-soluble vitamin b12. this study was to investigate co-fermentation of glycerol and fructose in soy-yogurt to enhance vitamin b12 production by lactobacillus reuteri. after a serial combination experiments, the co-fermentation was confirmed to enhance the production of vita ... | 2015 | 25955289 |
| study and use of the probiotic lactobacillus reuteri in pigs: a review. | probiotics are living microorganisms that provide a wide variety of health benefits to the host when ingested in adequate amounts. the bacterial strains most frequently used as probiotic agents are lactic acid bacteria, such as lactobacillus reuteri, which is one of the few endogenous lactobacillus species found in the gastrointestinal tract of vertebrates, including humans, rats, pigs and chickens. l. reuteri is one of the most well documented probiotic species and has been widely utilized as a ... | 2015 | 25954504 |
| probiotic supplements and debridement of peri-implant mucositis: a randomized controlled trial. | the aim of this double-blind randomized placebo-controlled trial was to evaluate the effects of probiotic supplements in adjunct to conventional management of peri-implant mucositis. | 2016 | 25953193 |
| lactobacillus reuteri i5007 modulates tight junction protein expression in ipec-j2 cells with lps stimulation and in newborn piglets under normal conditions. | tight junctions (tjs) maintain the intestinal mucosal barrier, dysfunction of which plays a vital role in the pathophysiology of a variety of gastrointestinal disorders. previously, we have shown that l. reuteri i5007 maintained the gut epithelial barrier in newborn piglets. here we aimed to decipher the influence of l. reuteri i5007 on tight junction (tj) protein expression both in vivo and in vitro. | 2015 | 25888437 |
| effectiveness of lactobacillus reuteri in infantile colic and colicky induced maternal depression: a prospective single blind randomized trial. | infant colic, excessive crying of unknown cause, is a major burden to families and effects about 10-30 % of infants. despite decades of research, the exact cause and treatment of infant colic has remained elusive. the use of lactobacillus reuteri (dsm 17938) in infant colic is somewhat controversial and hence, we designed this study to evaluate its efficacy in infantile colic. we recruited predominantly or exclusively breastfed infants, aged less than 4 months in a placebo controlled observation ... | 2015 | 25876529 |
| lactobacillus reuteri glyceraldehyde-3-phosphate dehydrogenase functions in adhesion to intestinal epithelial cells. | this study was aimed to identify key surface proteins mediating the adhesion of lactobacilli to intestinal epithelial cells. by using caco-2 and ipec-j2 cells labeled with sulfo-nhs-biotin in the western blotting, a protein band of an approximately 37 kda was detected on the surface layer of lactobacillus reuteri strains zj616, zj617, zj621, and zj623 and lactobacillus rhamnosus gg. mass spectrometry analysis using the adhesion-related protein from l. reuteri zj617 showed that it was 100% homolo ... | 2015 | 25867279 |
| effect of the probiotic bacterium lactobacillus reuteri on white spot lesion development in orthodontic patients. | the purpose of the study is to evaluate the effect of daily intake of lozenges containing probiotic bacteria on white spot lesion (wsl) formation as well as on salivary lactobacilli (lb) and mutans streptococci (ms) counts, in patients undergoing orthodontic treatment with fixed appliances. | 2016 | 25840585 |
| efficient production of reuterin from glycerol by magnetically immobilized lactobacillus reuteri. | reuterin is a compound that contains the monomeric, hydrated monomeric, and cyclic dimeric forms of 3-hydroxypropionaldehyde (3-hpa). it is widely used as a food preservative due to its antimicrobial properties, and 3-hpa is also an important precursor for several industrial chemicals. in this study, we first developed an efficient immobilization process involving magnetic cell entrapment for production of reuterin from glycerol by lactobacillus reuteri. the cell growth condition was optimized b ... | 2015 | 25805344 |
| preventive effect of lactobacillus reuteri crl1324 on group b streptococcus vaginal colonization in an experimental mouse model. | to assess the preventive effect of different intravaginal (i.va.) doses of lactobacillus reuteri crl1324 against vaginal colonization by group b streptococcus (gbs) in a murine experimental model. | 2015 | 25786121 |
| probiotic interventions in infantile colic. | up until 2014, the use of probiotics in infantile colic has shown promise. however, the past year has seen the publication of controversial results and rigorous debate on whether probiotics are effective in infantile colic. it is time to review the evidence and discuss whether probiotics should be used for infants with colic. | 2015 | 25769063 |
| growth and tolerance of term infants fed formula with probiotic lactobacillus reuteri. | lactobacillus reuteri has been studied for its safety and beneficial effects in infants. this study assessed growth of infants fed a partially hydrolyzed whey formula with l reuteri. | 2015 | 25758426 |
| clinical and biochemical evaluation of lozenges containing lactobacillus reuteri as an adjunct to non-surgical periodontal therapy in chronic periodontitis. | this study evaluates the effects on clinical and biochemical parameters of lactobacillus reuteri-containing probiotic supplementation adjunctive to initial periodontal therapy in patients with chronic periodontitis (cp). | 2015 | 25741580 |
| the effect of orally administered probiotic lactobacillus reuteri-containing tablets in peri-implant mucositis: a double-blind randomized controlled trial. | probiotics create a biofilm and protect the oral tissues against the action of periodontal pathogenic bacteria. the aim of this study was to evaluate the effects of the oral probiotic lactobacillus reuteri prodentis upon the peri-implant health of edentulous patients with dental implants and peri-implant mucositis, establishing comparisons vs implants without peri-implant disease. | 2015 | 25712760 |
| changes in bile acids, fgf-19 and sterol absorption in response to bile salt hydrolase active l. reuteri ncimb 30242. | the size and composition of the circulating bile acid (ba) pool are important factors in regulating the human gut microbiota. disrupted regulation of ba metabolism is implicated in several chronic diseases. bile salt hydrolase (bsh)-active lactobacillus reuteri ncimb 30242, previously shown to decrease ldl-cholesterol and increase circulating ba, was investigated for its dose response effect on ba profile in a pilot clinical study. ten otherwise healthy hypercholesterolemic adults, recruited fro ... | 2015 | 25612224 |
| prevention of functional gastrointestinal disorders in neonates: clinical and socioeconomic impact. | infantile colic, gastro-oesophageal reflux and constipation are the most common functional gastrointestinal disorders (fgids) affecting infants during the first months of life. despite infantile colic, functional constipation and regurgitation had a self-limited pattern, they are considered a risk factor for developing different disorders later in life. the pathophysiology of these functional diseases is still controversial but there is growing evidence that an abnormal gut microbiota colonisati ... | 2015 | 25609653 |
| are probiotics or prebiotics useful in pediatric irritable bowel syndrome or inflammatory bowel disease? | treatment options for irritable bowel syndrome (ibs) and inflammatory bowel disease (ibd) are notoriously either inadequate (ibs) or loaded with potentially serious side effects and risks (ibd). in recent years, a growing interest in effective and safer alternatives has focused on the potential role of probiotics and their metabolic substrates, prebiotics. it is in fact conceivable that the microbiome might be targeted by providing the metabolic fuel needed for the growth and expansion of benefi ... | 2014 | 25593899 |
| improved 1,3-propanediol synthesis from glycerol by the robust lactobacillus reuteri strain dsm 20016. | various lactobacillus reuteri strains were screened for the ability to convert glycerol to 1,3- propanediol (1,3-pdo) in a glycerol-glucose co-fermentation. only l. reuteri dsm 20016, a well-known probiotic, was able to efficiently carry out this bioconversion. several process strategies were employed to improve this process. co(2+) addition to the fermentation medium, led to a high product titer (46 g/l) of 1,3-pdo and to improved biomass synthesis. l. reuteri dsm 20016 produced also ca. 3 μg/g ... | 2015 | 25588555 |
| truncation of domain v of the multidomain glucansucrase gtf180 of lactobacillus reuteri 180 heavily impairs its polysaccharide-synthesizing ability. | glucansucrases are exclusively found in lactic acid bacteria and synthesize a variety of α-glucans from sucrose. they are large multidomain enzymes belonging to the cazy family 70 of glycoside hydrolase enzymes (gh70). the crystal structure of the n-terminal truncated gtf180 of lactobacillus reuteri 180 (gtf180-δn) revealed that the polypeptide chain follows a u shape course to form five domains, including domains a, b, and c, which resemble those of family gh13 enzymes, and two extra and novel ... | 2015 | 25586581 |
| normal growth of infants receiving an infant formula containing lactobacillus reuteri, galacto-oligosaccharides, and fructo-oligosaccharide: a randomized controlled trial. | the safety of an infant formula containing a new mixture of the prebiotics galacto-oligosaccharides (gos) and fructo-oligosaccharide (fos) and the probiotic lactobacillus reuteri needs to be evaluated. | 2015 | 27057326 |
| the effect of lactobacillus reuteri supplementation in adults with chronic functional constipation: a randomized, double-blind, placebo-controlled trial. | there is a growing interest for the use of probiotics for chronic constipation. a recent randomized controlled trial (rct) showed a positive effect of lactobacillus reuteri (l. reuteri) on bowel movement frequency in infants with chronic constipation. the aim of the present study was to evaluate the effects of l. reuteri in adult patients with functional constipation. | 2014 | 25531996 |
| preventive effects of oral probiotic on infantile colic: a prospective, randomised, blinded, controlled trial using lactobacillus reuteri dsm 17938. | infants were recruited in four centres in north-west italy. 138 infants were assessed for eligibility, 113 ones underwent randomisation and 105 completed the study. newborns aged less than 10 days of life, with gestational age between 37 and 42 weeks, birth weight from 2,500 to 4,300 g and normal physical examination were recruitable. premature infants and infants affected by outcomes of perinatal hypoxia or necrotising enterocolitis have been excluded. patients were randomly assigned to receive ... | 2015 | 25488262 |
| lactobacillus reuteri dsm 17938 for managing infant colic: protocol for an individual participant data meta-analysis. | infant colic, or excessive crying of unknown cause in infants less than 3 months old, is common and burdensome. its aetiology is undetermined, and consensus on its management is still lacking. recent studies suggest a possible link between infant colic and gut microbiota, indicating probiotics to be a promising treatment. however, only a few strains have been tested, and results from randomised controlled trials are conflicting. it is important to clarify whether probiotics are effective for tre ... | 2014 | 25475244 |
| semicarbazide-functionalized resin as a new scavenger for in situ recovery of 3-hydroxypropionaldehyde during biotransformation of glycerol by lactobacillus reuteri. | 3-hydroxypropionaldehyde (3hpa), a potential c3-platform chemical for a biobased industry, is produced from glycerol using lactobacillus reuteri through its glycerol dehydratase activity. however, the process is characterized by low yield and productivity due to toxic effects of 3hpa on the biocatalyst activity. in this study, a semicarbazide-functionalized resin was prepared, evaluated for adsorption and in situ recovery of 3hpa during biotransformation of glycerol. adsorption of 3hpa onto the ... | 2014 | 25456063 |
| probiotics for infantile colic: a randomized, double-blind, placebo-controlled trial investigating lactobacillus reuteri dsm 17938. | to investigate the effectiveness of lactobacillus reuteri dsm 17938 for the treatment of infantile colic in breastfed canadian infants, compared with placebo. | 2015 | 25444531 |
| isomalto/malto-polysaccharide, a novel soluble dietary fiber made via enzymatic conversion of starch. | dietary fibers are at the forefront of nutritional research because they positively contribute to human health. much of our processed foods contain, however, only small quantities of dietary fiber, because their addition often negatively affects the taste, texture, and mouth feel. there is thus an urge for novel types of dietary fibers that do not cause unwanted sensory effects when applied as ingredient, while still positively contributing to the health of consumers. here, we report the generat ... | 2014 | 25412115 |
| probiotic supplementation in systemic nickel allergy syndrome patients: study of its effects on lactic acid bacteria population and on clinical symptoms. | the study aimed to evaluate the effects of probiotic lactobacillus reuteri dsm 17938 strain supplementation in patients suffering from systemic nickel allergy syndrome, in terms of modulation of faecal lab population linked to a reduction of gi and cutaneous symptoms and to an increase of patient's quality of life. | 2015 | 25363062 |
| soluble factors from lactobacillus reuteri crl1098 have anti-inflammatory effects in acute lung injury induced by lipopolysaccharide in mice. | we have previously demonstrated that lactobacillus reuteri crl1098 soluble factors were able to reduce tnf-α production by human peripheral blood mononuclear cells. the aims of this study were to determine whether l. reuteri crl1098 soluble factors were able to modulate in vitro the inflammatory response triggered by lps in murine macrophages, to gain insight into the molecular mechanisms involved in the immunoregulatory effect, and to evaluate in vivo its capacity to exert anti-inflammatory act ... | 2014 | 25329163 |
| efficacy of synbiotics to reduce acute radiation proctitis symptoms and improve quality of life: a randomized, double-blind, placebo-controlled pilot trial. | to evaluate whether the daily intake of synbiotics interferes in radiation-induced acute proctitis symptoms and in quality of life in patients with prostate cancer. | 2014 | 25304789 |
| evaluation of biochemical and molecular methods for lactobacillus reuteri strains differentiation. | several biochemical and molecular methods were used for discrimination of four lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. internal transcribed spacer (its)-pcr method and protein analysis by sds-page and maldi-tof showed to be suitable for strain discrimination whereas its-pcr/rflp and enterobacterial repetitive intergenic consensus (eric)-pcr were not strain specific. the used methods differentiated tested strains into distinct groups; however, the location of ... | 2015 | 25300353 |
| residue leu940 has a crucial role in the linkage and reaction specificity of the glucansucrase gtf180 of the probiotic bacterium lactobacillus reuteri 180. | highly conserved glycoside hydrolase family 70 glucansucrases are able to catalyze the synthesis of α-glucans with different structure from sucrose. the structural determinants of glucansucrase specificity have remained unclear. residue leu(940) in domain b of gtf180, the glucansucrase of the probiotic bacterium lactobacillus reuteri 180, was shown to vary in different glucansucrases and is close to the +1 glucosyl unit in the crystal structure of gtf180-δn in complex with maltose. herein, we sh ... | 2014 | 25288798 |
| hypocholesterolemic effects of lactobacillus reuteri lr6 in rats fed on high-cholesterol diet. | the bacterium lactobacillus reuteri lr6, an isolate from breast-fed human infant feces, was tested positive for bile tolerance and bile salt hydrolase activity. it was also evaluated as a potential probiotic with cholesterol-lowering effect in vivo. in this study, 32 male albino rats were divided into four groups consisting of eight mice per group. for 60 d, group i was fed with normal synthetic diet, group ii was fed with cholesterol-enriched diet only, group iii was fed with cholesterol-enrich ... | 2015 | 25265203 |
| comparison of lactobacillus reuteri and nystatin prophylaxis on candida colonization and infection in very low birth weight infants. | the aim of this study was to compare the efficacy of orally administered lactobacillus reuteri (l. reuteri) versus nystatin in prevention of fungal colonization and invasive candidiasis in very low birth weight infants. | 2015 | 25245226 |
| effects of lactobacillus reuteri-derived biosurfactant on the gene expression profile of essential adhesion genes (gtfb, gtfc and ftf) of streptococcus mutans. | streptococci are the main causative agents in plaque formation and mutans streptococci are the principle etiological agent of dental plaque and caries. the process of biofilm formation is a step-wise process, starting with adhesion of planktonic cells to the surfaces. it is now a well known fact that expression of glucosyltransferases (gtfs) and fructosyltransferase (ftf) genes play a critical role in the initial adhesion of streptococcus mutans to the tooth surface, which results in the formati ... | 2014 | 25221772 |
| characterization of two truncated forms of xylanase recombinantly expressed by lactobacillus reuteri with an introduced rumen fungal xylanase gene. | the xylanase r8 gene (xynr8) from uncultured rumen fungi was cloned and successfully expressed in lactobacillus reuteri. a xylanase activity of 132.1 u/ml was found in the broth of l. reuteri r8, the transformant containing pnz3004 vector with xynr8 gene insertion. two distinct forms of recombinant xylanase with different hydrophobicities and molecular weights were found in the broth after purification. according to the results of western blotting, only the t7-tag, fused in the n-terminus of xyn ... | 2014 | 25152410 |
| short communication: combined antimicrobial activity of reuterin and diacetyl against foodborne pathogens. | reuterin (β-hydroxypropionialdehyde) is a broad-spectrum antimicrobial substance produced by some strains of lactobacillus reuteri during anaerobic fermentation of glycerol. some of these strains are able to survive and produce reuterin in cheese and yogurt when added as adjuncts to the starter. similarly, in fermented dairy foods, other inhibitory compounds such as lactic acid and diacetyl are produced during fermentation. in this work, we studied the combined effect of reuterin and diacetyl un ... | 2014 | 25087026 |
| an improved method for the electrotransformation of lactic acid bacteria: a comparative survey. | an efficient method for genetic transformation of lactic acid bacteria (lab) by electroporation is presented in this work. a comparative survey with other electrotransformation methods already published showed that the method proposed here yields the higher electrotransformation efficiency in the 12 lab strains tested, which could make the method applicable to other lab species or genera. | 2014 | 25086179 |
| crispr-cas9-assisted recombineering in lactobacillus reuteri. | clustered regularly interspaced palindromic repeats (crisprs) and the crispr-associated (cas) nuclease protect bacteria and archeae from foreign dna by site-specific cleavage of incoming dna. type-ii crispr-cas systems, such as the streptococcus pyogenes crispr-cas9 system, can be adapted such that cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. here we have developed and optimized crispr-cas9 function in the lactic acid bacterium lactobacillus re ... | 2014 | 25074379 |
| lactobacillus reuteri 100-23 modulates urea hydrolysis in the murine stomach. | comparisons of in vivo (mouse stomach) and in vitro (laboratory culture) transcriptomes of lactobacillus reuteri strain 100-23 were made by microarray analysis. these comparisons revealed the upregulation of genes associated with acid tolerance, including urease production, in the mouse stomach. inactivation of the urec gene reduced the acid tolerance of strain 100-23 in vitro, and the mutant was outcompeted by the wild type in the gut of ex-lactobacillus-free mice. urine analysis showed that st ... | 2014 | 25063664 |
| 2-butanol and butanone production in saccharomyces cerevisiae through combination of a b12 dependent dehydratase and a secondary alcohol dehydrogenase using a tev-based expression system. | 2-butanol and its chemical precursor butanone (methyl ethyl ketone--mek) are chemicals with potential uses as biofuels and biocommodity chemicals. in order to produce 2-butanol, we have demonstrated the utility of using a tev-protease based expression system to achieve equimolar expression of the individual subunits of the two protein complexes involved in the b12-dependent dehydratase step (from the pdu-operon of lactobacillus reuteri), which catalyze the conversion of meso-2,3-butanediol to bu ... | 2014 | 25054226 |
| fermentable non-starch polysaccharides increases the abundance of bacteroides-prevotella-porphyromonas in ileal microbial community of growing pigs. | most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (nsp). the knowledge about effects of these sources of nsp on the gut microbiota and its fermentation products is still scarce. the aim of this study was to investigate effects of feeding diets with native sources of nsp on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (scfa) and lactic acid. the experimen ... | 2014 | 25046106 |
| development of a non-dairy probiotic fermented product based on almond milk and inulin. | a new fermented almond "milk" that combined the properties of both almonds and probiotics was considered to cover the current versatile health-promoting foods' demand. almond milk fermentation with probiotic lactobacillus reuteri and streptococcus thermophilus was studied by using a central composite design with response surface methodology, and different factors (glucose, fructose, inulin and starters) were optimised to assure high probiotic survivals in the final product. the optimal formulati ... | 2015 | 25028153 |
| high-level expression and characterization of recombinant acid urease for enzymatic degradation of urea in rice wine. | ethylcarbamate, a carcinogenic compound, is formed from urea and ethanol in rice wine, and enzymatic elimination of urea is always attractive. in the present work, we amplified the acid urease gene cluster ureabcefgd from lactobacillus reuteri cicc6124 and constructed robust lactococcus lactis cell factories for the production of acid urease. the titer of the recombinant acid urease was increased from 1,550 to 11,560 u/l by optimization of the cultivation process. meanwhile, the enzyme showed sa ... | 2015 | 25027572 |
| evaluation of lactobacilli from human sources for uptake and accumulation of selenium. | the trace mineral selenium (se) is currently in demand for health supplements for human and animal nutrition. in the present study, 25 isolates of lactobacillus species of human origin from indian population were screened for their ability to uptake and accumulate se in a more bioavailable form. total se accumulated by cells was measured by inductively coupled plasma emission spectrometry (icpes) after wet digestion of se-enriched cultures. ten out of 25 isolates grew luxuriantly, as red pigment ... | 2014 | 25022245 |
| effect of lineage-specific metabolic traits of lactobacillus reuteri on sourdough microbial ecology. | this study determined the effects of specific metabolic traits of lactobacillus reuteri on its competitiveness in sourdoughs. the competitiveness of lactobacilli in sourdough generally depends on their growth rate; acid resistance additionally contributes to competitiveness in sourdoughs with long fermentation times. glycerol metabolism via glycerol dehydratase (gupcde) accelerates growth by the regeneration of reduced cofactors; glutamate metabolism via glutamate decarboxylase (gadb) increases ... | 2014 | 25015888 |
| lactobacillus reuteri is an effective option for the prevention of diarrhoea in preschool children but may not be cost-effective in all settings. | | 2014 | 25015434 |
| pre- and postnatal administration of lactobacillus reuteri decreases tlr2 responses in infants. | mice models indicate that intact toll like receptor (tlr) signaling may be essential for the allergy protective effects of diverse bacterial exposure observed in clinical trials and epidemiological studies. probiotic supplementation with lactobacillus reuteri from pregnancy week 36 and to the infant through the first year of life decreased the prevalence of ige-associated eczema at two years (clinicaltrials.gov nct01285830). the effect of this supplementation on innate immune responses to bacter ... | 2014 | 25002964 |
| biopreservation of fior di latte cheese. | in this study a new biopreservation system consisting of an active sodium alginate coating containing lactobacillus reuteri applied to fior di latte cheese was studied. the final aim was to extend cheese shelf life by the in situ production of reuterin. experimental trials were carried out with and without glycerol. how the fermentation time could improve the production of reuterin, enabling fior di latte shelf life, was also assessed. to this aim, the experimental analyses were conducted in 2 d ... | 2014 | 24952776 |
| from prediction to function using evolutionary genomics: human-specific ecotypes of lactobacillus reuteri have diverse probiotic functions. | the vertebrate gut symbiont lactobacillus reuteri has diversified into separate clades reflecting host origin. strains show evidence of host adaptation, but how host-microbe coevolution influences microbial-derived effects on hosts is poorly understood. emphasizing human-derived strains of l. reuteri, we combined comparative genomic analyses with functional assays to examine variations in host interaction among genetically distinct ecotypes. within clade ii or vi, the genomes of human-derived l. ... | 2014 | 24951561 |
| glutamine, glutamate, and arginine-based acid resistance in lactobacillus reuteri. | this study aimed to determine whether glutamine deamidation improves acid resistance of lactobacillus reuteri, and to assess whether arginine, glutamine, and glutamate-mediated acid resistance are redundant or complementary mechanisms of acid resistance. three putative glutaminase genes, gls1, gls2, and gls3, were identified in l. reuteri 100-23. all three genes were expressed during growth in mmrs and wheat sourdough. l. reuteri consistently over-expressed gls3 and the glutamate decarboxylase g ... | 2014 | 24929734 |
| prevention of late blowing defect by reuterin produced in cheese by a lactobacillus reuteri adjunct. | in this study, reuterin-producing lactobacillus reuteri inia p572 was added to cheese as an adjunct culture together with 50 or 100 mm glycerol (required for reuterin production), with the aim of controlling clostridium tyrobutyricum cect 4011 growth and preventing the late blowing defect (lbd) of cheese caused by this strain. l. reuteri survived cheese manufacture and produced reuterin in situ, detected at 6 and 24 h. however, the produced reuterin was enough to inhibit the growth of clostridiu ... | 2014 | 24929721 |
| effect of tablets containing probiotic bacteria (lactobacillus reuteri) on early caries lesions in adolescents: a pilot study. | the objective of the study was to investigate the effect of tablets containing probiotic lactobacilli on early caries lesions in adolescents with quantitative light-induced fluorescence (qlf). 36 healthy adolescents of both sexes (12-17 years of age) were enrolled and randomly allocated to a placebo-controlled trial with two parallel groups. the test group received two tablets daily containing two strains of lactobacillus reuteri (dsm 17938 and atcc pta 5289) for a period of three months, while ... | 2014 | 24889893 |
| fermentation conditions influence the fatty acid composition of the membranes of lactobacillus reuteri i5007 and its survival following freeze-drying. | lactobacillus reuteri i5007 has well-documented adhesion properties and health benefits. future industrial use of lact. reuteri i5007 will require the development of effective fermentation procedures and high bacterial survival following drying. therefore, this study was conducted to determine the impact of altering fermentation ph and temperature on the fatty acid composition of the bacterial membranes and subsequent survival of lact. reuteri i5007 following freeze-drying. initially, a response ... | 2014 | 24888635 |
| flux analysis of the lactobacillus reuteri propanediol-utilization pathway for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol. | lactobacillus reuteri converts glycerol to 3-hydroxypropionic acid (3hp) and 1,3-propanediol (1,3pdo) via 3-hydroxypropionaldehyde (3hpa) as an intermediate using enzymes encoded in its propanediol-utilization (pdu) operon. since 3hp, 1,3pdo and 3hpa are important building blocks for the bio-based chemical industry, l. reuteri can be an attractive candidate for their production. however, little is known about the kinetics of glycerol utilization in the pdu pathway in l. reuteri. in this study, t ... | 2014 | 24886501 |
| lactobacillus reuteri dsm 17938 differentially modulates effector memory t cells and foxp3+ regulatory t cells in a mouse model of necrotizing enterocolitis. | necrotizing enterocolitis (nec) is an inflammatory disease with evidence of increased production of proinflammatory cytokines in the intestinal mucosa. lactobacillus reuteri dsm 17938 (lr17938) has been shown to have anti-inflammatory activities in an experimental model of nec. activated effector lymphocyte recruitment to sites of inflammation requires the sequential engagement of adhesion molecules such as cd44. the phenotype of cd44(+)cd45rb(lo) separates t effector/memory (tem) cells from nai ... | 2014 | 24852566 |
| characterization of the most abundant lactobacillus species in chicken gastrointestinal tract and potential use as probiotics for genetic engineering. | the count and diffusion of lactobacilli species in the different gastrointestinal tract (gi) regions of broilers were investigated by quantitative real-time polymerase chain reaction, and the probiotic characteristics of six l. reuteri species isolated from broilers' gi tract were also investigated to obtain the potential target for genetic engineering. lactobacilli had the highest diversity in the crop and the lowest one in the cecum. compared with the lower gi tract, more lactobacilli were fou ... | 2014 | 24850302 |
| oral lactobacillus reuteri gmn-32 treatment reduces blood glucose concentrations and promotes cardiac function in rats with streptozotocin-induced diabetes mellitus -- retraction. | | 2014 | 24829954 |
| probiotic vs. placebo in irritable bowel syndrome:a randomized controlled trial. | this study assesses the potential effect of lactobacillus reuteri as a single strain probiotic preparation (biogaia®) on irritable bowel syndrome (ibs). | 2013 | 24829677 |
| the efficacy of lactobacillus reuteri dsm 17938 in infants and children: a review of the current evidence. | we aimed to systematically evaluate evidence on the effectiveness of lactobacillus reuteri dsm 17938 (l. reuteri) for treating and preventing diseases in infants and children. medline and the cochrane library were searched in december 2013, with no language restrictions, for relevant randomized controlled trials (rcts) and meta-analyses. the search was updated in april 2014. one systematic review and 14 rcts met the inclusion criteria. the use of l. reuteri may be considered in the management of ... | 2014 | 24819885 |
| a randomized double blind controlled safety trial evaluating d-lactic acid production in healthy infants fed a lactobacillus reuteri-containing formula. | d-lactic acidosis in infants fed lactic acid bacteria-containing products is a concern. | 2014 | 24812520 |
| structural investigation of a neutral extracellular glucan from lactobacillus reuteri sk24.003. | the structural features of a neutral extracellular glucan derived from lactobacillus reuteri sk24.003 were investigated. colonies of the strain sk24.003 exhibited a creamy and slimy morphological appearance on mrs solid medium and were identified as l. reuteri via 16s rdna sequence analysis. the exopolysaccharide produced from sucrose was composed exclusively of glucose, and the weight-average molecular weight was 4.31 × 10(7)g/mol. the polysaccharide exhibited an α-(1→4) backbone with an α-(1→6 ... | 2014 | 24721093 |
| enhancement of lactobacillus reuteri kub-ac5 survival in broiler gastrointestinal tract by microencapsulation with alginate-chitosan semi-interpenetrating polymer networks. | to evaluate an alginate-chitosan microcapsule for an intestinal probiotic delivery system for broilers. | 2014 | 24712513 |
| diarrhoea. lactobacillus reuteri reduces episodes of diarrhoea in healthy children. | | 2014 | 24709814 |
| complete genome sequence of lactobacillus reuteri i5007, a probiotic strain isolated from healthy piglet. | lactobacillus reuteri i5007 is a well-characterized probiotic strain isolated from the colonic mucosa of healthy weaning piglets. here, we present the complete genome sequence of this strain, which consists of a circular chromosome and six distinct plasmids. | 2014 | 24685642 |
| probiotic l. reuteri treatment prevents bone loss in a menopausal ovariectomized mouse model. | estrogen deficiency is a major risk factor for osteoporosis that is associated with bone inflammation and resorption. half of women over the age of 50 will experience an osteoporosis related fracture in their lifetime, thus novel therapies are needed to combat post-menopausal bone loss. recent studies suggest an important role for gut-bone signaling pathways and the microbiota in regulating bone health. given that the bacterium lactobacillus reuteri atcc pta 6475 (l. reuteri) secretes beneficial ... | 2014 | 24677054 |
| probiotic 'glow of health': it's more than skin deep. | radiant skin and hair are universal indicators of good health. it was recently shown that feeding of probiotic bacteria to aged mice rapidly induced youthful vitality characterised by thick lustrous skin and hair, and enhanced reproductive fitness, not seen in untreated controls. probiotic-treated animals displayed integrated immune and hypothalamic-pituitary outputs that were isolated mechanistically to microbe-induced anti-inflammatory interleukin-10 and neuropeptide hormone oxytocin. in this ... | 2014 | 24675231 |
| the growth of streptococcus mutans in different milks for infant feeding. | after birth various bacterial species, mainly streptococci, colonize the oral cavity and are frequently isolated from carious lesions in children. previous studies suggest that an earlier colonization of a child's mouth by cariogenic organisms might be related to a higher risk of caries. the aim of this study is to assess the influence of different milks for infant feeding on the growth of streptococcus mutans (sm) colonies. three human milk samples from three different mothers and five infant f ... | 2017 | 24674690 |
| a functional analysis of the formyl-coenzyme a (frc) gene from lactobacillus reuteri 100-23c. | to examine the role of the lactobacillus reuteri 100-23c frc gene product in oxalate metabolism, host colonization and the acid stress response. | 2014 | 24655128 |
| diarrhea in preschool children and lactobacillus reuteri: a randomized controlled trial. | to evaluate whether daily administration of lactobacillus reuteri dsm 17938 reduces the frequency and duration of diarrheal episodes and other health outcomes in day school children in mexico. | 2014 | 24639271 |
| improvement of digestive health and reduction in proteobacterial populations in the gut microbiota of cystic fibrosis patients using a lactobacillus reuteri probiotic preparation: a double blind prospective study. | although scientific knowledge about the benefits of probiotic use in cystis fibrosis (cf) is scarce, their expectative is promising. the aim of this work was to analyze the effect of a lactobacillus reuteri probiotic preparation versus placebo in cf patients. | 2014 | 24636808 |
| the early administration of lactobacillus reuteri dsm 17938 controls regurgitation episodes in full-term breastfed infants. | forty breastfed full-term infants were randomly, double blind assigned to receive orally lactobacillus reuteri (l. reuteri) dsm 17938, 5 drops/daily (10(8) colony-forming units), for 4 weeks (n = 20) or an identical placebo (n = 20), starting before third day of life. they underwent basal and final visit to monitor growth parameters and gastrointestinal (gi) disease. parents registered daily: crying minutes, stool frequency and consistency, numbers of regurgitations, adverse events. secretory ig ... | 2014 | 24635827 |
| flexibility of truncated and full-length glucansucrase gtf180 enzymes from lactobacillus reuteri 180. | glucansucrase enzymes synthesize high-molecular-mass extracellular α-glucan polysaccharides from sucrose. previously, the crystal structure of truncated glucansucrase glucosyltransferase (gtf)180-δn from lactobacillus reuteri 180 (lacking the n-terminal domain) revealed an elongated overall structure with two remote domains (iv and v) extending away from the core. by contrast, a new crystal form of the α-1,6/α-1,3 specific glucansucrase gtf180-δn shows an approximate 120(o) rotation of domain v ... | 2014 | 24597929 |
| structural and molecular insights into novel surface-exposed mucus adhesins from lactobacillus reuteri human strains. | the mucus layer covering the gastrointestinal tract is the first point of contact of the intestinal microbiota with the host. cell surface macromolecules are critical for adherence of commensal bacteria to mucus but structural information is scarce. here we report the first molecular and structural characterization of a novel cell-surface protein, lar_0958 from lactobacillus reuteri jcm 1112(t) , mediating adhesion of l. reuteri human strains to mucus. lar_0958 is a modular protein of 133 kda co ... | 2014 | 24593252 |
| development of a strain-specific real-time pcr assay for enumeration of a probiotic lactobacillus reuteri in chicken feed and intestine. | a strain-specific real-time pcr assay was developed for quantification of a probiotic lactobacillus reuteri (dsm 16350) in poultry feed and intestine. the specific primers were designed based on a genomic sequence of the strain derived from suppression subtractive hybridization with the type strain l. reuteri dsm 20016. specificity was tested using a set of non-target strains from several sources. applicability of the real-time pcr assay was evaluated in a controlled broiler feeding trial by usi ... | 2014 | 24587284 |
| lactobacillus reuteri dsm 17938 effectively reduces the duration of acute diarrhoea in hospitalised children. | guidelines consider certain probiotics useful in the management of acute gastroenteritis. this study evaluated the use of lactobacillus (l) reuteri dsm 17938. | 2014 | 24579935 |
| biosynthesis of 1,3-propanediol from glycerol with lactobacillus reuteri: effect of operating variables. | chemical synthesis of 1,3-propanediol (1,3-pd) is environmentally unfriendly and hence its microbial production is preferred, especially for biomedical, cosmetic and textile applications. in this work, production of 1,3-pd by co-fermentation of glucose and glycerol by lactobacillus reuteri was investigated under different cultivation conditions such as aeration, acetate concentration and different molar ratios of glucose/glycerol. the final concentration of 1,3-pd and yield attained under unaera ... | 2014 | 24525111 |