| the characterization of nonbacterial prostatitis: search for an etiology. | nonbacterial prostatitis is often difficult to differentiate from other prostatic complaints and remains a vaguely characterized syndrome. prostatic fluid inflammatory cells and elevated immunoglobulins raise the suspicion that this syndrome is caused by some undetected infection. prostatic fluid antibodies against chlamydia trachomatis, ureaplasma urealyticum, staphylococcus, staphylococcus faecalis, bacteroides fragilis and clostridium perfringens were measured in men with nonbacterial and bac ... | 1992 | 1279213 |
| sialyl ligands facilitate lymphocyte accumulation during inflammation of the central nervous system. | combined models of cytokine-induced inflammation in the skin and spinal cord of the rat were utilised to demonstrate in vivo that circulating lymphocytes depend upon sialylated adhesion molecules on their surface for maximal recruitment into inflammatory sites in both tissues. when radiolabelled normal spleen cells were incubated with sialidase from vibrio cholerae or clostridium perfringens, or with the specific sialic acid-binding lectin from limax flavus, prior to being washed and injected in ... | 1992 | 1281823 |
| in vitro evaluation of bay y3118, a new full-spectrum fluoroquinolone. | bay y3118, 1-cyclopropyl-7-(2,8-diazabicyclo[4.3.0]non-8-yl)-6-fluoro-8- chloro-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid hydrochloride, is a new fluoroquinolone with antibacterial activity against an expanded spectrum of species including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, streptococcus pneumoniae, streptococcus pyogenes, and also anaerobes such as bacteriodes fragilis and clostridium perfringens. mic90s for s. aureus, s. epidermid ... | 1992 | 1288961 |
| clostridial sepsis with massive intravascular hemolysis: rapid diagnosis and successful treatment. | a 61-year-old man developed a pyrescia accompanied by a massive intravascular hemolysis after abdominal surgery (whipple's operation) of a pancreatic adenocarcinoma. abdominal ultrasound and the abdominal ct-scan showed marked aerobilia and multiple liver abscesses. laboratory tests demonstrated the presence of the thomsen-friedenreich cryptantigen (tca) on the membranes of the patient's erythrocytes. the enzymatic cleavage of n-acetyl-neuraminic acid usually covering the tca may lead to a life ... | 1992 | 1289375 |
| effect of hyperbaric oxygen and surgery on experimental gas gangrene. | an experimental model of clostridial gas gangrene was developed in rats and the therapeutic value of surgical debridement alone versus a combination of surgery and hyperbaric oxygen (hbo) was assessed. the infection was produced by an intramuscular injection of clostridium perfringens microorganisms. the mortality of untreated rats was 100%. the mortality of the rats treated only with surgery was 37.5% compared to 12.5% when hbo was added to the treatment protocol (p < 0.01). in the group treate ... | 1992 | 1292939 |
| resistance of anaerobic bacteria to antimicrobial agents in spain. | as a consequence of antibiotic consumption, the entire microbial ecosystem attached to man is evolving towards resistance. in spain, penicillin resistance (mic > 0.5 mg/l) is found in about 10% of peptostreptococcus, clostridium perfringens and eubacterium, and in 50% of veillonella. cefoxitin resistance ( > 2 mg/l) is present in 10 to 20% of peptostreptococcus and clostridium, and in 50% of eubacterium; 21% of bacteroides (fragilis group) strains are resistant to 16 mg/l. a very low rate of imi ... | 1992 | 1295754 |
| antibacterial in vitro-activity of meropenem against 200 clinical isolates in comparison to 11 selected antibiotics. | the antimicrobial activity of meropenem, a new parenteral carbapenem, was tested in vitro by an agar dilution method against 200 clinical isolates (gram-negative/positive aerobes and anaerobes). meropenem was compared with imipenem, ceftazidime, cefotaxime, piperacillin, ciprofloxacin, gentamicin; and metronidazole, cefoxitin, chloramphenicol, clindamycin, vancomycin when appropriate. meropenem and imipenem exhibited an extended spectrum of activity with low minimal inhibitory concentrations (mi ... | 1992 | 1303691 |
| activation of pge2-secretion from gastric mucosa by a type i phospholipase c is mediated by a direct release of arachidonic acid. | we investigated the effects of an exogenous type i phospholipase c (plc) from clostridium perfringens on arachidonic acid release and prostaglandin synthesis from gastric mucosa by determining pge2 release from organ cultured rabbit mucosal biopsies as well as pge2 synthesis and substrate-dependent inactivation of the prostaglandin cyclooxygenase from endogenously released arachidonic acid in mucosal homogenate. plc dose dependently stimulated pge2 secretion from organ cultured mucosa to 145% an ... | 1992 | 1304434 |
| [isolation of clostridium perfringens from a wound in a case of post-traumatic gas gangrene. diagnostics remarks]. | the authors described a case of post traumatic gas gangrene in a young man. rapid clinical and microbiological diagnosis with isolation of clostridium strain, and immediate therapy, including hyperbaric chamber, brought complete recovery without incapacitation. | 1992 | 1305783 |
| [upper respiratory tract infections as a hypothetical source of sudden infant death syndrome (sids)]. | a hypothesis that the common bacterial toxins are the possible cause of the sudden infant death syndrome (sids) was discussed. recently, even botulinum toxin and clostridium perfringens type a enterotoxin are mentioned. | 1992 | 1305788 |
| [production of a vaccine against enterotoxemia from clostridium perfringens strains isolated in the field]. | we have isolated eight strains of c. perfringens from cases of enterotoxaemia. five of these strains have revealed themselves toxic with respective types (type "a":2, type "c":2, type "d":1). in order to produce anti-enterotoxaemia vaccine, we have proceeded at the cultivation in fermenter of isolated strains and reference strains cwa 35, cwc and cwd af. at the end of fermentation, we have evaluated the two following parameters: obtained biomass, and toxin titers. with the two classes of strains ... | 1992 | 1309137 |
| nucleotide sequence of the lecithinase operon of listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. | the lecithinase gene of the intracellular pathogen listeria monocytogenes, plcb, was identified in a 5,648-bp dna fragment which expressed lecithinase activity when cloned into escherichia coli. this fragment is located immediately downstream of the previously identified gene mpl (prta). it contains five open reading frames, named acta, plcb, and orfx, -y, and -z, which, together with mpl, form an operon, since a 5.7-kb-long transcript originates from a promoter located upstream of mpl (j. menga ... | 1992 | 1309513 |
| adp-ribosylation of gelsolin-actin complexes by clostridial toxins. | adp-ribosylation of the 1:1 (g-a) and 1:2 (g-a-a) gelsolin-actin complexes by clostridium perfringens iota toxin and clostridium botulinum c2 toxin was studied. iota toxin adp-ribosylated actin in the g-a complex from human platelets as effectively as skeletal muscle actin. the km for nad (4 microm) was identical for both substrates. c2 toxin adp-ribosylated actin in the g-a complex with lower efficacy than nonmuscle actin from platelet cytosol. in the g-a-a complex both actin molecules were adp ... | 1992 | 1309748 |
| effect of phospholipase treatment on insulin receptor signal transduction. | to study the role of membrane lipids in signal transduction by the insulin receptor, we have studied the effect of phospholipase c (clostridium perfringens) and a phosphatidylinositol-specific phospholipase (staphylococcus aureus) on insulin binding, a function of the alpha-subunit, and tyrosine kinase activity, a function of the beta-subunit in im-9 lymphocytes and nih 3t3 fibroblasts transfected with the human insulin receptor. treatment of the cells with phospholipase c at concentrations up t ... | 1992 | 1312492 |
| susceptibility of anaerobic bacteria to pd 131628. | the in vitro activity of pd 131628 against anaerobic cocci, propionibacterium acnes, clostridium perfringens, clostridium difficile, bacteroides fragilis, bacteroides spp. and fusobacteria was determined by the agar dilution method. this activity was compared with that of ciprofloxacin, piperacillin, cefoxitin, imipenem, clindamycin, metronidazole and chloramphenicol. pd 131628, imipenem, clindamycin, metronidazole and chloramphenicol were the most active agents tested. | 1992 | 1314176 |
| enteric clostridium perfringens infection associated with parvoviral enteritis in dogs: 74 cases (1987-1990). | parvovirus infection was confirmed by fluorescent antibody staining of or viral isolation from specimens of small intestine in 181 (17%) of 1,110 dogs necropsied between july 1, 1987 and dec 31, 1990. clostridium perfringens was isolated from 74 (69%) of 108 dogs with parvovirus infection from which specimens of jejunum also had been obtained for culture of anaerobic bacteria. gram-positive bacilli in association with focal to diffuse necrosis of the superficial portions of the villi were observ ... | 1992 | 1315727 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| inhibition of neuromuscular transmission in isolated mouse phrenic nerve-diaphragm by the enterotoxin of clostridium perfringens type a. | the enterotoxin of clostridium perfringens type a, a channel forming protein toxin, inhibited neuromuscular transmission under conditions of low calcium. twitch tension of isolated phrenic nerve-diaphragm preparations elicited by electrical stimulations to the phrenic nerve was recorded isometrically, and the preparations were exposed to the purified enterotoxin. in krebs solution containing 0.5 mm calcium, the enterotoxin (20 micrograms/ml) reduced within 10 min the amplitude of the twitch tens ... | 1992 | 1326137 |
| mobilization of diacylglycerol in intact hela cells by exogenous phospholipase c from cl. perfringens is accompanied by release of fatty acids including arachidonic acid. | the second messenger diacylglycerol (dag), chiefly derived from phosphatidylcholine (pc) or from phosphatidylinositol (pi), through the activation of specific phospholipases c (plc), plays a key role in cellular stimulation. the activation of a particular plc was simulated in intact hela cells by treatment with exogenous pc-plc (cl. perfringens) or with pi-plc (b. cereus). both enzymes rapidly mobilized dag. however, only pc-plc led, in hela cells, to morphological changes (which were reversible ... | 1992 | 1327153 |
| isolation of inclusion bodies from vegetative clostridium perfringens: partial purification of a 47 kda inclusion protein. | a refractile inclusion body produced by vegetative cells of clostridium perfringens at temperatures above 40 degrees c was isolated and partially characterized. the inclusion was composed of protein and could be solubilized by sodium dodecyl sulphate plus either dithiothreitol or beta-mercaptoethanol. the solubilized inclusion showed no antigenic relationship with cl. perfringens enterotoxin. one major band with an apparent mw of 47 kda was demonstrated after polyacrylamide gel electrophoresis o ... | 1992 | 1328131 |
| the in-vitro activity of new streptogramins, rp 59500, rp 57669 and rp 54476, alone and in combination. | rp 59500 is a 30:70 mixture of rp 57669 and rp 54476. the activity of rp 59500 and its two components against gram-positive and gram-negative organisms was compared with that of clarithromycin, roxithromycin, azithromycin and rokitamycin. rp 59500 inhibited 90% of erythromycin-susceptible and resistant staphylococcus aureus and coagulase-negative staphylococci at less than or equal to 1 mg/l (range 0.06-2 mg/l). both inducibly and constitutively-resistant strains of s. aureus, as well as strains ... | 1992 | 1328138 |
| mutations adjacent to the dimple of the canine parvovirus capsid structure affect sialic acid binding. | the erythrocyte receptor on rhesus macaque erythrocytes used by canine parvovirus (cpv) for binding in hemagglutination (ha) was examined. erythrocyte membrane proteins were electrophoresed and blotted to nitrocellulose and probed with [125i]-labeled cpv capsids, showing seven virus-binding proteins. treatment of erythrocytes or isolated membranes with clostridium perfringens neuraminidase virtually abolished virus binding. binding was also affected by treatment with potassium periodate and inhi ... | 1992 | 1329321 |
| severe heart muscle degeneration caused by clostridium perfringens type a in camel calves (camelus dromedarius). | clostridium perfringens type a was isolated from different organs and intestines from three to five weeks old camel calves which have died from heart muscle necrosis. no other bacterial pathogens were isolated. virus isolation on two different cell lines including a fetal camel skin were also negative. mice which were injected with bacteria free filtrates prepared from intestinal contents of necropsied camel calves died after one to six hours demonstrating the presence of clostridial toxins. our ... | 1992 | 1339990 |
| [bacteria associated with diarrheic episodes in young camels in niger]. | during diarrhoeic episodes of young camels in niger, bacteria such as salmonella enteritidis, e. coli, staphylococcus sp., clostridium perfringens and other bacteria were isolated from stool samples. bacteria roll in this pathology is discussed, and a treatment is proposed, compared with antibiogram results. | 1992 | 1345079 |
| cloning of hsp60 (groel) operon from clostridium perfringens using a polymerase chain reaction based approach. | using degenerate oligonucleotide primers for conserved regions of hsp60, a 0.6 kilobase fragment of clostridium perfringens dna was amplified by the polymerase chain reaction. the amplified fragment was used as a probe to isolate a genomic clone containing the c. perfringens hsp60 operon. the clone contained two open reading frames homologous to the groes and groel (or hsp60) family of bacterial and eukaryotic proteins as well as other upstream and downstream sequences. the approach described he ... | 1992 | 1347462 |
| fatal postoperative gastric necrosis caused by clostridium perfringens. | | 1992 | 1356487 |
| a highly sensitive enzyme-linked immunosorbent assay for clostridium perfringens enterotoxin. | a highly sensitive enzyme-linked immunosorbent assay (elisa) for quantitation of clostridium perfringens enterotoxin (cpe) by a sandwich method with polystyrene beads was elaborated. the elisa was very sensitive with a detection limit of 1 pg/ml of cpe. clostridium perfringens culture fluid did not interfere with the assay. this elisa may be useful for the mass screening for cl. perfringens producing small amounts of cpe. | 1992 | 1368348 |
| stimulation of the onset of sporulation of clostridium perfringens type a by netropsin and distamycin. | the basic peptide antibiotics, netropsin and distamycin, previously shown to inhibit sporulation of bacillus subtilis, stimulated low levels of sporulation of clostridium perfringens strain nctc 8798 at concentrations of 1.0 and 0.1 microgram/ml respectively. most sporulating cells produced in the presence of the antibiotics were defective. these were blocked at stage iii of sporulation, and many possessed forespores exterior to the sporangium. the same antibiotics could also inhibit the caffein ... | 1992 | 1369407 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of clostridium perfringens type a. | enterotoxin-positive (ent+) and enterotoxin-negative (ent-) strains of clostridium perfringens were cultured in duncan-strong sporulation medium containing starch at 37 and 46 degrees c. at 37 degrees c, all strains degraded starch and sporulated well. however, only ent- strains could hydrolyze starch, grow extensively, and sporulate at 46 degrees c. growth, sporulation, and starch hydrolysis by ent+ strains at 46 degrees c were equivalent to those obtained at 37 degrees c when alpha-amylase was ... | 1992 | 1371660 |
| mapping of functional regions of clostridium perfringens type a enterotoxin. | studies were conducted to allow construction of an initial map of the structure-versus-function relationship of the clostridium perfringens type a enterotoxin (cpe). removal of the n-terminal 25 amino acids of cpe increased the primary cytotoxic effect of cpe but did not affect binding. cpe sequences required for at least four epitopes were also identified. | 1992 | 1373406 |
| phylogenetic analysis of the pathogenic anaerobe clostridium perfringens using the 16s rrna nucleotide sequence. | clostridium perfringens, the first pathogenic clostridium examined, was placed in the nonmycoplasma subgroup of the low-dg+dc-content gram-positive cluster on the basis of the results of a phylogenetic analysis in which we used 16s rrna comparisons. the closest relative that has been identified to date is clostridium pasteurianum. | 1992 | 1374625 |
| the influence of neuraminidase treatment on tracheal smooth muscle contraction. | to examine the role of sialic acid in the respiratory tract, the influence of neuraminidase from clostridium perfringens was investigated on contractions of isolated guinea-pig and rat trachea and on histamine release from guinea-pig lung tissue. treatment with 2.0 units/ml of neuraminidase at 37 degrees c and ph 7.4 for 30 min caused an approximately 60% removal of total n-acetylneuraminic acid, a representative sialic acid, from muscle from guinea-pig and rat trachea. neuraminidase concentrati ... | 1992 | 1385181 |
| physical map of the listeria monocytogenes chromosome. | the circular physical map of the pathogenic bacterium listeria monocytogenes lo28 (serovar 1/2c) was established by using pulsed-field gel electrophoresis. the l. monocytogenes chromosome contains eight noti fragments of 1,100, 940, 400, 335, 280, 45, 30, and 20 kb in size and eight sse8387i fragments of 860, 680, 680, 370, 335, 130, 70, and 25 kb. therefore, the total length of the genome is 3,150 kb. to order the noti fragments on the chromosome, we used a strategy which can be of general use. ... | 1992 | 1385384 |
| metastatic endophthalmitis caused by clostridium perfringens. | a case of metastatic endophthalmitis due to clostridium perfringens originating from the biliary tract is reported. the grave visual prognosis and the importance of early detection and treatment of the primary source of infection are emphasised. | 1992 | 1390500 |
| impact of the advances in age on the gastrointestinal microflora of beagle dogs. | the gastrointestinal microflora of male beagle dogs in two different age groups; i) less than month 12 of age, and ii) more than year 11 of age, was compared. no detectable difference occurred on the microflora of stomach, duodenum, jejunum, and ileum in both dogs. large bowel (cecum, colon and rectum) microflora in both dogs yielded the different microbial populations. in all regions of large bowel, the levels of bacteroides, eubacteria, peptostreptococci, bifidobacteria, lactobacilli, and stap ... | 1992 | 1391181 |
| cloning of tetracycline-resistance genes from various strains of clostridium perfringens and expression in escherichia coli. | one hundred strains of clostridium perfringens and 52 strains of other clostridia of human and animal origins were screened for tetracycline resistance. fifty-six strains were resistant to tetracycline in the c. perfringens group. ten strains were selected for their high level of resistance. in all of them, the tetracycline-resistance genes were found to be residing in large plasmids of about 50 kb, all showing homologies. several tetracycline-resistance genes from plasmids of various strains of ... | 1992 | 1393823 |
| effect of diolein on hydrolysis of phosphatidylcholine by phospholipase c from clostridium perfringens. | the activity of phospholipase c from clostridium perfringens on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (popc) as a monolayer at an air/water interface was examined. with a pure popc monolayer, sharp cut-off of the enzyme activity was observed on increase in surface pressure. however, this cut-off disappeared on addition of a 0.3 molar fraction of 1,2-dioleoylglycerol (1,2-do) to the monolayer. an abrupt change in the enzyme activity was observed with molar fractions of between 0.2 and ... | 1992 | 1400264 |
| an outbreak of enterotoxaemia in cats. | eighteen local breed cats suffered from enterotoxaemia after scavenging on chicken-remnants. they suffered from vomitus and diarrhoea and collapsed dead after a few hours. an untypable strain of clostridium perfringens was isolated in pure form and was suspected to be the cause of the outbreak. | 1992 | 1414094 |
| the influence of diet on necrotic enteritis in broiler chickens. | necrotic enteritis was reproduced in broiler chickens by mixing cultures of clostridium perfringens in the feed. mortality due to necrotic enteritis was higher among chickens fed rations based on wheat, rye, barley, and oat groats than among chickens fed corn-based rations. addition of pentosanase to a wheat-based diet did not affect the level of mortality due to necrotic enteritis. addition of pectin and guar gum to different rations severely reduced growth rate and eliminated necrotic enteriti ... | 1992 | 1417581 |
| necrotic enteritis in turkeys. | three flocks of turkey hens (16,000 each) between 7 and 12 weeks of age experienced outbreaks of necrotic enteritis. necropsy revealed a dilated duodenum and jejunum with mucosal surfaces covered with a diphtheritic membrane. intestinal scrapings showed very few oocysts of eimeria sp. histopathological findings were compatible with necrotic enteritis but with deeper, more severe lesions than in broiler chickens. clostridium perfringens was isolated by anaerobic culture from the intestinal conten ... | 1992 | 1417616 |
| the prevalence of salmonella infections in camels (camelus dromedarius) in the united arab emirates. | one hundred and eighty-seven salmonella strains were isolated from 4006 samples collected from camels in the united arab emirates between 1987 and 1991. one hundred and sixty-five (4.3%) strains were isolated from 3801 faecal and 22 from 205 organ samples of 62 autopsied camels. in total, 28 different serotypes were identified with s. saintpaul being the most frequent (69), followed by s. frintrop (31) and s. hindmarsh (15). salmonella typhimurium was isolated from only two faecal specimens. all ... | 1992 | 1422785 |
| use of bacitracin in the prevention and treatment of experimentally-induced idiopathic colitis in horses. | ten healthy ponies from a single herd were found by repeated fecal culture to be free of salmonella species and clostridium cadaveris. in a preliminary study, four ponies administered a single oral dose of 10 mg/kg lincomycin did not develop idiopathic colitis when the drug was administered alone. four other ponies were administered 10 mg/kg lincomycin by stomach tube together with 0.45 l of colonic content from a horse with idiopathic colitis induced earlier by lincomycin alone. two of the four ... | 1992 | 1423060 |
| emphysematous cystitis due to clostridium perfringens and candida albicans in two patients with hematologic malignant conditions. | fever, abdominal pain, and hematuria developed in two patients with hematologic malignant conditions (multiple myeloma and agnogenic myeloid metaplasia). each patient was found to have emphysematous cystitis (ec), secondary to clostridium perfringens and candida albicans, respectively. both patients had debilitated general medical conditions, compromised immune function, prior treatment with broad-spectrum antibiotics and corticosteroids, bladder outlet obstruction, and indwelling foley catheter ... | 1992 | 1423196 |
| in vitro antimicrobial activity of the new antibiotic vermisporin. | the antimicrobial activity of vermisporin, a new antibiotic produced by fermentation of the fungus ophiobolus vermisporis, was tested in vitro. vermisporin inhibited 90% of bacteroides fragilis and other bacteroides spp. at 1 microgram/ml (range 0.25-1 micrograms/ml). clostridium perfringens were inhibited by 1 microgram/ml (range 0.25-2 micrograms/ml). vermisporin inhibited 90% of staphylococcus aureus, including methicillin-resistant staphylococcus aureus, at 0.5 micrograms/ml (range 0.12-0.5 ... | 1992 | 1425739 |
| in vitro activity of l-627 against anaerobic bacteria. | the in vitro activity of l-627 against 370 anaerobic bacterial strains including anaerobic cocci, propionibacterium acnes, clostridium perfringens, clostridium difficile, bacteroides fragilis, other bacteroides spp. and fusobacteria was determined by the agar dilution method. this activity was compared with that of piperacillin, cefoxitin, imipenem, meropenem, clindamycin, metronidazole and chloramphenicol. l-627, imipenem, meropenem, clindamycin, metronidazole and chloramphenicol were the most ... | 1992 | 1425740 |
| spontaneous bacterial peritonitis due to clostridium perfringens in a patient with liver cirrhosis and pure red cell aplasia. | a 63-year-old man with decompensated liver cirrhosis and pure red cell aplasia complained of pyrexia, abdominal distention and abdominal pain. a diagnosis of spontaneous bacterial peritonitis (sbp), conn's syndrome, was made upon the isolation of an anaerobe clostridium perfringens from both ascitic fluid and peripheral blood. the bacteria were found to be susceptible to piperacillin, and administration of the antimicrobial agent markedly improved his sbp. the anaerobes should be kept in mind as ... | 1992 | 1426858 |
| comparison of the nucleotide sequence and development of a pcr test for the epsilon toxin gene of clostridium perfringens type b and type d. | the sequence of the epsilon toxin gene of clostridium perfringens type d was determined and compared with that of the previously reported type b sequence. it showed two nucleotide changes in the open reading frame, giving rise to one amino acid substitution. the promoter sequences were not homologous, and different putative -35 and -10 regions have been identified in each. the sequence information was used to develop pcr primers which were specific for the epsilon toxin gene. the utility of this ... | 1992 | 1427007 |
| desferrioxamine potentiated sod antiinflammatory activity in rat adjuvant arthritis: role of iron and bacterial toxins. | in this paper we studied the modulating inflammatory activity of iron in the adjuvant arthritis, taking indomethacin as a standard antiinflammatory drug and a superoxide dismutase derivative (mpeg-sod) as a scavenger of free radicals. moreover, we evaluated the changes in potential intestinal pathogens requiring iron for growth, in order to study the role of bacteria in the altered gastrointestinal functions observed during arthritis. we observed a 50% arthritis inhibition on the 14th day with m ... | 1992 | 1442328 |
| an immunohistochemical study of plasma albumin extravasation in the brain of mice after the administration of clostridium perfringens type d epsilon toxin. | | 1992 | 1445075 |
| [specific immunity in patients with inflammatory diseases of the abdominal cavity]. | in most patients with pyoinflammatory diseases of the abdominal cavity caused by cl. perfringens microbial associations accumulation of agglutinins occurred during the disease. in purulent abdominal processes caused by cl. perfringens microbial associations a connection exists between the increased antibody level and wound suppuration. the rise of the antibody level was most marked, whatever the character of the bacterial associations, in patients with generalized peritonitis, abdominal abscess, ... | 1992 | 1447884 |
| clostridium perfringens septicemia during the course of leukemia. | clostridium perfringens (cp) is an infrequent cause of septicemia in neutropenic patients. we report the case of a 17 year old man treated for acute lymphoblastic leukaemia in whom a strain of cp was isolated from blood cultures. the clinical spectrum and evolution of cp septicemia in neutropenic patients are discussed. | 1992 | 1448356 |
| antibiotic susceptibility of anaerobic bacteria isolated in johannesburg. | in vitro susceptibilities of 198 anaerobic bacteria to seven antibiotics were evaluated by the agar dilution method. in addition to testing amoxycillin/clavulanic acid in a 2:1 ratio against the bacteria, the combination was also tested against 63 isolates using fixed concentrations of clavulanic acid and serial dilutions of amoxycillin. penicillin and cefoxitin were not effective against beta-lactamase-producing bacteroides isolates and only 50% of isolates were susceptible to the 2:1 amoxycill ... | 1992 | 1448710 |
| high-level production and purification of escherichia coli n-acetylneuraminic acid aldolase (ec 4.1.3.3). | the escherichia coli gene which encodes n-acetylneuraminic acid aldolase was isolated by the polymerase chain reaction, cloned into the inducible expression vector pttq18, and overexpressed in e. coli. the high yield of aldolase was achieved through both optimum growth of cells and efficient expression of the aldolase gene (20-30% soluble cellular protein). the recombinant enzyme was purified to homogeneity with an activity of 1.2-2.2 u/mg, which compared favorably with that of commercial prepar ... | 1992 | 1458058 |
| bacterial vaccines: old and new, veterinary and medical. | developments in veterinary and medical bacterial vaccines are outlined. in the former case, economic considerations are paramount, and cruder, less purified products of proven efficacy continue to be employed. for human use, however, safety and absence of side effects are increasingly demanded. various examples of human and veterinary vaccines are discussed, and interaction between the two fields is illustrated by reference to the pig-bel disease in new guinea and the possible aetiology of sudde ... | 1992 | 1471428 |
| experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems. | the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ... | 1992 | 1472363 |
| the nucleotide sequence of a putative membrane transport gene from clostridium perfringens. | a gene from clostridium perfringens encoding a highly hydrophobic polypeptide of m(r) = 30,698 was cloned and sequenced. the polypeptide showed similarities with the inner membrane proteins of binding protein-dependent transport systems of the gram negative enterobacteria. the sequence homology to the ugpe and malg proteins of escherichia coli was 27.6%, or 60.4 and 52.2%, respectively, taking into account the conservative amino acid changes. the polypeptide contained the eaa---g---------(i/v)-l ... | 1992 | 1472712 |
| syntheses and activities of n-substituted derivatives of siastatin b. | n-substituted derivatives of siastatin b have been obtained by a chemical modification. some derivatives showed potent inhibitory activities against streptococcus sp. and clostridium perfringens neuraminidases. | 1992 | 1473994 |
| necrotizing lesions in the intestine, gizzard, and liver in captive capercaillies (tetrao urogallus) associated with clostridium perfringens. | during the period from 1982 to 1991, 863 captive and 32 wild capercaillies (tetrao urogallus) were necropsied. the most common cause of death in captive capercaillies was necrotizing enteritis, diagnosed in 110 (13%) birds. of these, 31 (28%) birds also had necrotizing lesions in the liver. necrotizing gastritis occurred in 29 birds, two of which had concurrent necrotizing enteritis. in the capercaillies with necrotizing enteritis, clostridium perfringens type a was isolated more frequently and ... | 1992 | 1474658 |
| clostridium perfringens outbreak associated with minestrone soup. | clostridium perfringens food poisoning usually occurs after animal protein foods such as meat or poultry are consumed. in november 1990, an outbreak of gastrointestinal illness occurred among attendees at a conference of cake decorators held in michigan. all 42 conference attendees were surveyed. thirty-two of 42 (76%) attendees reported gastrointestinal symptoms. the median incubation period was 6.5 hours (range, 1.5-15 hours). ill persons were almost five times more likely to have consumed min ... | 1992 | 1476151 |
| detection of clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure. | the polymerase chain reaction (pcr) procedure was evaluated to see if it is a simple and rapid method to detect clostridium perfringens enterotoxin gene. the method, involving the use of two synthesised primers and gene amplification by the pcr procedure, detects a dna fragment of 364 base pairs of c. perfringens enterotoxin gene by gel electrophoresis. the enterotoxin gene of strains was detected by use of purified chromosomal dna. the supernatant of sporulating cultures in a sporulating medium ... | 1992 | 1476867 |
| application of pyrolysis mass spectrometry to the investigation of outbreaks of food poisoning and non-gastrointestinal infection associated with bacillus species and clostridium perfringens. | eighteen isolates of bacillus species and 15 of clostridium perfringens, all of which had been associated with outbreaks of either food poisoning or non-gastrointestinal infection (ngi), were examined for relatedness by pyrolysis mass spectrometry (pyms). the pyms-analysis correctly clustered all the groups of epidemiologically related isolates of both genera, and distinguished all the single, epidemiologically unrelated isolates of the same species. pyms is a simple, rapid and inexpensive techn ... | 1992 | 1476868 |
| [bacterial translocation in crohn disease]. | bacterial translocation is the passage of viable endogenous bacteria from the gastrointestinal tract to mesenteric lymph nodes and other internal organs. the aim of this work was to study bacterial translocation in patients operated on for crohn's disease. twenty-eight patients, mean age 29 years, not having received any antibiotics since at least 8 days, presenting with ileal (n = 12), ileo-colonic (n = 14) or colonic (n = 2) crohn's disease were studied. in 25 out of 28 cases (89%) indication ... | 1992 | 1478405 |
| the natural course of clostridium perfringens--induced pneumatosis cystoides intestinalis. | primary pneumatosis cystoides intestinalis (pci) is an uncommon, usually benign condition whose natural course is poorly understood and which can sometimes produce significant changes in a patient's cecum and sigmoid colon. in this study, pci was produced by monocontaminating the peritoneal cavities of adult germfree rats with clostridium perfringens. these animals were then observed for up to 26 weeks. pci took up to two weeks to develop, lasted at least ten weeks in most animals, and presumabl ... | 1992 | 1479305 |
| mechanisms of the cytopathic action of actin-adp-ribosylating toxins. | clostridium botulinum c2 toxin, clostridium perfringens iota toxin, and clostridium spiroforme toxin adp-ribosylate actin monomers. toxin-induced adp-ribosylation disturbs the cellular equilibrium between monomeric and polymeric actin and traps monomeric actin in its unpolymerized form, thereby depolymerizing actin filaments and destroying the microfilament network. furthermore, the toxins adp-ribosylate gelsolin actin complexes. these modifications may contribute to the cytopathic action of the ... | 1992 | 1479882 |
| toxin production by clostridium perfringens isolated from broiler chickens and capercaillies (tetrao urogallus) with and without necrotizing enteritis. | a total of 192 isolates of clostridium perfringens were isolated from 99 broiler chickens and 93 capercaillies (tetrao urogallus). fifty of the isolates from broilers and 44 of the isolates from capercaillies were from birds with necrotizing enteritis, and the remainder were from birds without this disease. the isolates were tested for the production of three major toxins (alpha, beta, and epsilon) and four minor toxins (theta, gelatinase, mu, and nu). all isolates were found to be c. perfringen ... | 1992 | 1485870 |
| comparative in vitro activity of ro 40-6890, ro 41-3399, and other antimicrobial agents against anaerobic bacteria. | the in vitro activity of the ester ro 41-3399 and its free active acid ro 40-6890 was tested against 189 strains of anaerobic bacteria in comparison to other oral cephalosporins and to antimicrobial agents established in the treatment of anaerobic infections. prevotella, porphyromonas, peptostreptococcus, fusobacterium and clostridium spp. were susceptible to ro 40-6890, with few exceptions. due to its lack of activity against the major pathogens of the bacteriodes fragilis group, ro 40-6890 doe ... | 1992 | 1486894 |
| a novel membrane-acting immunotoxin, the immunolysin, with therapeutic potential. | | 1992 | 1486988 |
| factors affecting the incidence of necrotic enteritis, caecal carriage of clostridium perfringens and bird performance in broiler chicks. | two trials were conducted to study the effects of a competitive exclusion (ce) product broilact and the anticoccidial narasin on the incidence of necrotic enteritis (ne), the numbers of clostridium perfringens (cp) in the caeca of broiler chicks and the performance of the birds. in trial 1 the effects of type of protein and partial replacement of a narasin containing diet with whole wheat were also studied. all groups of chicks were studied up to the point of slaughter at 43 days of age and afte ... | 1992 | 1488953 |
| comparative sequence analysis of the catb gene from clostridium butyricum. | sequence analysis of the clostridium butyricum chloramphenicol acetyltransferase (cat) gene, catb, showed that it encoded a cat monomer of 219 amino acids with a molecular weight of 26,114. comparison of the deduced amino acid sequence of the catb monomer to those of sixteen other cats showed that it was most closely related to the catq monomer from clostridium perfringens. | 1992 | 1489203 |
| effects of site-specific mutations on the enzymatic properties of a sialidase from clostridium perfringens. | three site-specific mutations were performed in two regions of a sialidase gene from clostridium perfringens which are known to be conserved in bacterial sialidases. the mutant enzymes were expressed in escherichia coli and, when measured with mu-neu5ac as substrate, exhibited variations in enzymatic properties compared with the wild-type enzyme. the conservative substitution of arg 37 by lys, located in a short conserved region upstream from the four repeated sequences common in bacterial siali ... | 1992 | 1490102 |
| antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin. | a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ... | 1992 | 1490908 |
| evaluation of enzyme-linked immunosorbent assay for diagnosis of clostridium perfringens enterotoxemias. | two double sandwich enzyme-linked immunosorbent assays (elisa) for clostridium perfringens beta and epsilon toxins were assessed for routine diagnosis of enterotoxemias on intestinal contents of 151 sheep that died suddenly. conventional tests (mouse assay and culture of organism) showed that 21 specimens were positive for clostridium perfringens type c (beta toxin) and 39 were positive for clostridium perfringens type d (epsilon toxin) enterotoxemias. comparison of the elisa results with conven ... | 1992 | 1496812 |
| a conjugated synthetic peptide corresponding to the c-terminal region of clostridium perfringens type a enterotoxin elicits an enterotoxin-neutralizing antibody response in mice. | a synthetic peptide homolog corresponding to the c-terminal 30 amino acids of clostridium perfringens type a enterotoxin (cpe) was conjugated to a thyroglobulin carrier and used to immunize mice. conjugate-immunized mice produced antibodies which neutralized native cpe cytotoxicity, at least in part, by blocking enterotoxin binding. this peptide may be useful for the development of a vaccine to protect against cpe-mediated disease. | 1992 | 1500207 |
| effect of lipidic factors on membrane cholesterol topology--mode of binding of theta-toxin to cholesterol in liposomes. | we have previously suggested the existence of two distinct states for cholesterol in cell membranes as revealed by high- and low-affinity binding sites for theta-toxin of clostridium perfringens. in liposomes, phospholipid and cholesterol compositions, but not membrane protein composition, have been shown to be major determinants for the topology of membrane cholesterol. the effects of lipidic factors on cholesterol topology were investigated in detail by analyzing toxin binding to large unilame ... | 1992 | 1504083 |
| bacteriology of ophthalmic infections with special reference to anaerobes. | a total of 138 eye specimens were processed for aerobic and anaerobic cultures. clinical data were obtained from 50 patients with unilateral ophthalmic infection. cultures from the uninfected eye of 38 of these 50 patients were also processed for comparison. in addition, 50 cultures were obtained from one or both eyes of 30 healthy controls who had no eye infection. anaerobes and aerobes were isolated from infected eyes of 6 (12%) and 37 (74%) patients respectively. no growth was observed in inf ... | 1992 | 1506066 |
| pathogens in neonatalomphalitis. | during a 3-year study period, 33 neonates with omphalitis (with proven cultures) were encountered; aerobic and anaerobic cultures were obtained. an incidence of 2/1000 live births was recorded, with a high prevalence rate of 15.6/1000 admissions noted. aerobes were isolated from 23 (70 per cent) specimens, whilst anaerobes were recovered alone in five (15 per cent) cases. there were 40 (1.2 per specimen) and 31 (0.9 per specimen) aerobic and anaerobic isolates, respectively. there was a signific ... | 1992 | 1507308 |
| antibacterial activity of n-[2-(dodecanoylmethylamine)ethyl]-alkyl dimethylammonium bromides. | the study contains the results of determination of antibacterial activity of the newly synthesized series of n-[2-(dodecanoylmethylamine)ethyl]-alkyldimethylammonium bromides. the efficiency of the compounds has been characterized towards three species of the genus clostridium such as clostridium perfringens, type a, clostridium bifermentans, clostridium sporogenes and one strain of the genus lactobacillus such as lactobacillus yamanashiensis. the antibacterial activity of the studied compounds ... | 1992 | 1509829 |
| nitroimidazoles, part xxiii--activity of satranidazole series against anaerobic infections. | a large number of nitroimidazoles have been examined for in vitro activity against three anaerobes - bacteroides fragilis (bf), a strain of bf resistant to metronidazole (16a) and clostridium perfringens and many found to be active. among these may be mentioned 1-methyl-5-nitroimidazoles carrying n - bound hetetocycles at position 2, such as satranidazole 1a, 1b, 1c, 1k, 1n and 1v which are at least twice as active as metronidazole (16a), ornidazole (16b) and tinidazole (16c). even more active a ... | 1992 | 1512026 |
| biosynthesis of a nonphysiological sialic acid in different rat organs, using n-propanoyl-d-hexosamines as precursors. | in this study it could be shown that in rat the normally occurring n-acetyl neuraminic acid can be modified in its n-acyl moiety by in vivo administration of the chemically synthesized n-propanoyl precursors, n-propanoyl-d-glucosamine or n-propanoyl-d-mannosamine. it could be shown that each of these nonphysiological amino sugar analogues was incorporated into both membrane and serum glycoproteins. after treatment of rats with radiolabeled n-[acyl-1-14c]d-mannosamine, radioactivity could be remo ... | 1992 | 1512235 |
| clostridium perfringens enterotoxin is a superantigen reactive with human t cell receptors v beta 6.9 and v beta 22. | candidate superantigens were screened for their ability to induce lysis of human histocompatibility leukocyte antigen class ii-positive targets by human cd8+ influenza-specific cytotoxic t cell (ctl) lines. clostridium perfringens enterotoxin (cpet) induced major histocompatibility complex unrestricted killing by some but not all ctl lines. using "anchored" polymerase chain reactions, cpet was shown to selectively stimulate peripheral blood lymphocytes bearing t cell receptor v beta 6.9 and v be ... | 1992 | 1512551 |
| immunological homology among azoreductases from clostridium and eubacterium strains isolated from human intestinal microflora. | azoreductases from several anaerobic intestinal bacteria have been shown to reduce azo dyes to carcinogenic aromatic amines. to evaluate the structural similarities of azoreductases from four species of clostridium and one species of eubacterium, a polyclonal antibody against purified clostridium perfringens azoreductase was generated in rabbits. this antibody inhibited the azoreductase activity of all five bacteria tested. elisa showed different degrees of binding of the antibody to various spe ... | 1992 | 1512704 |
| an activity which restores theta toxin activity in some theta toxin-deficient mutants of clostridium perfringens. | group a mutants of clostridium perfringens are deficient in theta toxin but release a dialyzable substance ("substance a"), which restores theta toxin activity to group b mutants, into a culture supernatant; group b mutants are defective in "substance a" release. "substance a" activity appeared in the exponentially growing phase of group a mutants and disappeared in the stationary phase. "substance a" activity was most stable at ph 5.0 and 0 c and even increased threefold in the first 5 hr, but ... | 1992 | 1513265 |
| construction of a sequenced clostridium perfringens-escherichia coli shuttle plasmid. | a new clostridium perfringens-escherichia coli shuttle plasmid has been constructed and its complete dna sequence compiled. the vector, pjir418, contains the replication regions from the c. perfringens replicon pip404 and the e. coli vector puc18. the multiple cloning site and lacz' gene from puc18 are also present, which means that x-gal screening can be used to select recombinants in e. coli. both chloramphenicol and erythromycin resistance can be selected in c. perfringens and e. coli since p ... | 1992 | 1513878 |
| multiple typing techniques applied to a clostridium perfringens food poisoning outbreak. | twenty-one stool specimens obtained from persons implicated in two food poisoning outbreaks at the same institution in smith falls, ontario, were examined for clostridium perfringens. ninety-two colonies of cl. perfringens (3-5 per stool specimen) were typed with antisera, bacteriocins and by plasmid analysis. they were also tested for the in vitro production of bacteriocin and enterotoxin. sixteen of the 21 stool specimens were tested directly for enterotoxin. this was detected in 13, five of w ... | 1992 | 1517172 |
| detection of clostridium perfringens and its enterotoxin in cases of sporadic diarrhoea. | to determine the incidence of sporadic and apparently non-food related diarrhoea associated with clostridium perfringens enterotoxin. | 1992 | 1517462 |
| influence of radiotherapy on intestinal microflora in cancer patients. | we investigated in 15 patients with carcinoma of the uterine cervix or endometrium, who were undergoing postoperative radiation therapy, the effects of different fractionated radiation exposures on counts of fecal bacteria, on the growth of clostridium difficile and clostridium perfringens enterotoxin production. we observed a generally significant decrease in intestinal microflora after the first radiation exposure, whereas at the end of radiotherapy all bacteria increased and reached basal val ... | 1992 | 1517812 |
| a 41.7 kda serine protease from clostridium perfringens type a: degradation of purified human serum proteins. | two clinical isolates of clostridium perfringens type a produced a novel caseinolytic serine protease. both enzymes had a molecular weight of 41.7 kilodaltons and an isoelectric point of 9.1. the two enzymes were immunogenic for rabbits and closely related serologically. both enzymes partially degraded the heavy chains of human immunoglobulins (ig) g and igm, but not iga. purified human complement (c) components c3, c5, c8, and c9 were attacked; c1q was refractory. both enzymes were active again ... | 1992 | 1520973 |
| role of the upstream region containing an intrinsic dna curvature in the negative regulation of the phospholipase c gene of clostridium perfringens. | the phospholipase c (alpha-toxin) gene (plc) of clostridium perfringens was cloned into puc19 and the effects of the upstream regions on expression of the plc gene were examined in escherichia coli jm109. when the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase c increased approximately 10-fold. northern blot hybridization analysis of the plc transcript showed that the upstream region inhibited transcription from the plc promoter. nucleot ... | 1992 | 1522810 |
| effect of p-chloromercuribenzoate on clostridium perfringens beta toxin. | p-chloromercuribenzoate (pcmb) was shown to bind to clostridium perfringens beta toxin. treatment of the toxin with n-ethylmaleimide (nem), 5,5'-dithio-bis(2-nitro-benzoic acid) (dtnb), o-iodosobenzoate (oiba) and metal ions such as cu2+ and ag+ decreased the lethal activity, but pcmb did not affect the lethal activity. on the other hand, the binding of pcmb to the toxin was inhibited by dtnb and nem in a dose-dependent manner. furthermore, the lethal activity of beta toxin pretreated with pcmb ... | 1992 | 1529464 |
| clostridial myositis in dogs. | | 1992 | 1529510 |
| sphingosine enhances platelet aggregation through an increase in phospholipase c activity by a protein kinase c-independent mechanism. | sphingosine (a potent inhibitor of protein kinase c) at 5-10 microm, which are concentrations lower than those that inhibit this enzyme activity, enhanced the aggregation of rabbit platelets induced by low concentrations of u46619, platelet-activating factor, thrombin and arachidonic acid, whereas h-7 and staurosporine, other protein kinase c inhibitors, failed to do so. of the sphingosine analogues which also inhibit protein kinase c, psychosine and lyso-gm3 did not show such an enhancing effec ... | 1992 | 1540140 |
| high-affinity binding of clostridium perfringens epsilon-toxin to rat brain. | 125i-epsilon-toxin showed high affinity to rat brain homogenates and synaptosomal membrane fractions, having single binding phases with dissociation constants (kds) of 2.5 and 3.3 nm, respectively. treatment of synaptosomal membrane fractions with pronase and neuraminidase lowered the binding of the labeled toxin, whereas treatment with trypsin and phospholipase c did not. heating of the fractions resulted in a decrease in the binding of the toxin. these data suggest that interaction of epsilon- ... | 1992 | 1541539 |
| purification and characterization of the lethal toxin (alpha-toxin) of clostridium septicum. | clostridium septicum lethal (alpha-toxin) was purified and found to be a basic protein (pi 8.4) of approximately 48 kda that is both lethal and hemolytic. the alpha-toxin had a hemolytic activity of approximately 2 x 10(7) hemolytic units per mg and a 50% lethal dose of approximately 10 micrograms/kg of body weight for mice. the alpha-toxin formed concentration-dependent, sodium dodecyl sulfate-resistant aggregates of approximately 230 kda. mice immunized with alpha-toxin showed a significant in ... | 1992 | 1541552 |
| elucidation of the topological parameters of n-acetylneuraminic acid and some analogues involved in their interaction with the n-acetylneuraminate lyase from clostridium perfringens. | a series of neuraminic acid derivatives modified in the side chain or at c-3, c-4 or c-5 were tested as substrates of inhibitors of n-acetylneuraminate lyase (ec 4.1.3.3) from clostridium perfringens. the results, together with km and ki values reported previously, indicate that the region most important for the binding of sialic acids is an equatorial zone reaching from c-8 via the ring oxygen atom to c-4 of the sugar molecule, whereas the substituents at c-9 and c-5 may be varied to a higher e ... | 1992 | 1546967 |
| regulation of the bacterial intestinal implantation in infant born by caesarean section. | studies were undertaken to determine the regulation of the bacterial intestinal implantation in 19 newborns delivered by caesarean section. correlation was made with the infant feeding mode. the effect of human milk seemed to be the result of b. bifidum proliferation, in contrast to artificial alimentation that seemed to favour c. perfringens implantation. the question was raised by us as to whether this opposition was only related to alimentation. in fact, b. bifidum itself also had an effect a ... | 1992 | 1547622 |
| physical and genetic mapping of the genomes of five mycoplasma hominis strains by pulsed-field gel electrophoresis. | we present the complete maps of five mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. the restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed-field gel electrophoresis. all the apai, smai, bamhi, xhoi, and sali restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. ... | 1992 | 1551841 |
| clostridial septicemia in an urban hospital. | the records of 56 patients at an urban hospital who had positive blood cultures for clostridia were reviewed. each patient was classified as immunologically normal or immunosuppressed. data were collected on clinical history, type of clostridial bacteremia, physical and laboratory determinants of infection, therapeutic intervention, clinical course and outcome. of the 56 patients, 22 were determined to be immunosuppressed. among all 56 patients, 28 had a malignancy, usually gastrointestinal or h ... | 1992 | 1553607 |
| emphysematous gastritis secondary to gastric infarction. | we report a 72-yr-old female hospitalized for an upper gastrointestinal hemorrhage who developed emphysematous gastritis and gas in the portal vein. endoscopy of the stomach revealed severe circumferential erythema, erosions, exudates, and friability of irregularly thickened proximal gastric folds. the patient became septic on the third day of hospitalization and deteriorated rapidly. computerized tomographic scan of the abdomen revealed extensive collections of gas within the gastric wall and i ... | 1992 | 1553943 |
| clostridial myonecrosis resulting from subcutaneous epinephrine suspension injection. | a 32-year-old asthmatic man developed a life-threatening clostridium perfringens infection after subcutaneous epinephrine suspension injection. extensive surgical debridement, including forequarter amputation, hyperbaric oxygen therapy, and appropriate antibiotics were used as life-saving measures. the patient made a fair functional recovery. we could find no previously reported cases of gas gangrene following subcutaneous injection in otherwise healthy individuals. the pathogenesis and treatmen ... | 1992 | 1554186 |