| cold active hydrolytic enzymes production by psychrotrophic bacilli isolated from three sub-glacial lakes of nw indian himalayas. | the diversity of culturable, cold-active enzymes producing bacilli was investigated from three sub-glacial lakes of north western indian himalayas. amplified ribosomal dna restriction analysis (ardra) using three restriction enzymes alu i, msp i, and hae iii led to the clustering of 136 bacilli into 26, 23, and 22 clusters at 75% similarity index from chandratal lake, dashair lake, and pangong lake, respectively. phylogenetic analysis based on 16s rrna gene sequencing led to the identification o ... | 2016 | 26933936 |
| induced maize salt tolerance by rhizosphere inoculation of bacillus amyloliquefaciens sqr9. | salt stress reduces plant growth and is now becoming one of the most important factors restricting agricultural productivity. inoculation of plant growth-promoting rhizobacteria (pgpr) has been shown to confer plant tolerance against abiotic stress, but the detailed mechanisms of how this occurs remain unclear. in this study, hydroponic experiments indicated that the pgpr strain bacillus amyloliquefaciens sqr9 could help maize plants tolerate salt stress. after exposure to salt stress for 20 day ... | 2016 | 26932244 |
| a new strategy to express the extracellular α-amylase from pyrococcus furiosus in bacillus amyloliquefaciens. | extracellular α-amylase from pyrococcus furiosus (pfa) shows great starch-processing potential for industrial application due to its thermostability, long half-life and optimal activity at low ph; however, it is difficult to produce in large quantities. in contrast, α-amylase from bacillus amyloliquefaciens (baa) can be produced in larger quantities, but shows lower stability at high temperatures and low ph. here, we describe a baa protein expression pattern-mimicking strategy to express pfa in ... | 2016 | 26916714 |
| the endo-β-1,4-glucanase of bacillus amyloliquefaciens is required for optimum endophytic colonization of plants. | the egls gene in bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. in this study, a disruption mutant of gene egls was constructed to examine its role in bacterial adaptation in plants. the mutant tb2k, egls gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. when inoculated on brassica campestris, the tb2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, ... | 2016 | 26907762 |
| bacillus amyloliquefaciens sqr9 induces dendritic cell maturation and enhances the immune response against inactivated avian influenza virus. | the objective of this study was to evaluate the stimulatory effects of bacillus amyloliquefaciens sqr9 on dendritic cells (dcs) and to verify its ability to enhance the immune response by modulating dc maturation. the results demonstrated that b. amyloliquefaciens sqr9 can adhere to the nasal epithelium and be taken up by dcs in the nasal mucosa, thereby inducing dc maturation and resulting in increased cd80, cd86, cd40 and mhcii expression and cytokine secretion. the frequencies of cd4(+) and c ... | 2016 | 26892720 |
| design, synthesis, and antimicrobial activities of new tanshinone iia esters. | sixteen new ester derivatives with various partition coefficient (clogp) values of tanshinone iia (tsiia), a major lipophilic component of salvia miltiorrhiza, were designed and synthesised, including six aliphatic esters (3a-e, 5a), one phosphate ester (4c) and nine aromatic esters (5b-j). their antimicrobial activities against three gram-positive bacteria strains, staphylococcus aureus, bacillus subtilis, and bacillus amyloliquefaciens, and two gram-negative bacteria strains, pseudomonas aerug ... | 2016 | 26829106 |
| draft genome sequence of bacillus amyloliquefaciens strain rhnk22, isolated from rhizosphere with biosurfactant (surfactin, iturin, and fengycin) and antifungal activity. | bacillus amyloliquefaciens strain rhnk22 isolated from groundnut rhizosphere showed direct and indirect plant growth-promoting traits along with biosurfactant activity and reduction in surface tension of water. biosurfactants were identified as lipopeptides (surfactin, iturin, and fengycin) by molecular and biochemical analysis in our studies. | 2016 | 26823600 |
| constitutive production and efficient secretion of soluble full-length streptavidin by an escherichia coli 'leaky mutant'. | due to its various applications the protein streptavidin is a highly interesting target for heterologous production. this study focuses on different escherichia coli-based constructs targeting a high-level expression and secretion of streptavidin to the medium. the effect of various promoters, variants of the target gene, leader sequences and host strains on expression and secretion into the culture broth was analyzed. constitutive production of full-length streptavidin fused with the leader seq ... | 2016 | 26820322 |
| plant-microbe communication enhances auxin biosynthesis by a root-associated bacterium, bacillus amyloliquefaciens sqr9. | mechanisms by which beneficial rhizobacteria promote plant growth include tryptophan-dependent indole-3-acetic acid (iaa) synthesis. the abundance of tryptophan in the rhizosphere, however, may influence the level of benefit provided by iaa-producing rhizobacteria. this study examined the cucumber-bacillus amyloliquefaciens sqr9 system and found that sqr9, a bacterium previously shown to enhance the growth of cucumber, increased root secretion of tryptophan by three- to fourfold. using a split-r ... | 2016 | 26808445 |
| [screening and antibacterial function of bacillus amyloliquefaciens x030]. | we isolated 339 bacillus strains from 72 soil samples all over the country, then purified their antimicrobial compounds and studied the antibacterial activity, to enrich bacillus resources and explore their second metabolites. | 2015 | 26762026 |
| engineering of a bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions. | the neutral protease has high potential for industrial applications, and attempts to improve enzyme expression level have important application values. in the present study, a neutral protease-encoding gene, banpr, was cloned from bacillus amyloliquefaciens strain k11, and a genetic manipulation method specific for this difficult-to-transform strain was developed for the high-level expression of neutral protease. the recombinant plasmid pub110-banpr was constructed in bacillus subtilis strain wb ... | 2016 | 26752595 |
| uv photolysis for enhanced phenol biodegradation in the presence of 2,4,6-trichlorophenol (tcp). | a bacterial strain isolated from activated sludge and identified as bacillus amyloliquefaciens could biodegrade phenol, but 2,4,6-trichlorophenol (tcp) inhibited phenol biodegradation and biomass growth. uv photolysis converted tcp into dichlorocatechol, monochlorophenol, and dichlorophenol, and this relieved inhibition by tcp. phenol-removal and biomass-growth rates were significantly accelerated after uv photolysis: the monod maximum specific growth rate (μ(max)) increased by 9% after tcp phot ... | 2016 | 26747251 |
| the antibiosis action and rice-induced resistance, mediated by a lipopeptide from bacillus amyloliquefaciens b014, in controlling rice disease caused by xanthomonas oryzae pv. oryzae. | in the present study, a lipopeptide (named axlp14) antagonistic to xanthomonas oryzae pv. oryzae (xoo) was obtained from the culture supernatant of bacillus amyloliquefaciens b014. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that axlp14 consisted of surfactin homologs. the minimum inhibition concentration and minimum bactericidal concentration of axlp14 against xoo were determined to be 1.25 and 2.50 mg/ml, respectively. at a concentration o ... | 2016 | 26718470 |
| immobilization of bacillus amyloliquefaciens sp1 and its alkaline protease in various matrices for effective hydrolysis of casein. | an extracellular alkaline protease producing b. amyloliquefaciens sp1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. b. amyloliquefaciens sp1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. enzyme activity before immobilization (at 60 °c, ph 8.0 for 5 min) was 3580 µg/ml/min. the results of immobilization with various matrices revealed ... | 2016 | 28330279 |
| bacillus amyloliquefaciens biofilm as a novel biosorbent for the removal of crystal violet from solution. | bacillus amyloliquefaciens biofilm shows promise for use in the control of soil-borne pathogens; however, it has never been used to treat dye-polluted wastewaters. here, we propose the novel idea of using b. amyloliquefaciens biofilm for the adsorption of crystal violet (cv) from liquids. the relative contents of three main elements (c1s, o1s, and n1s) in the biofilm were 65.55%, 21.21%, and 13.24%, respectively. the results of fourier transform infrared (ftir) spectra and x-ray photoelectron sp ... | 2016 | 26707697 |
| gene cloning, expression, and characterization of the bacillus amyloliquefaciens ps35 lipase. | lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. in an attempt to characterize lipases at molecular level from novel sources, a lipase gene from bacillus amyloliquefaciens ps35 was cloned, heterologously expressed in escherichia coli dh5α cells and sequenced. it showed up to 98% homology with other lipase sequences in the ncbi database. the recombinant enzyme was then purified from e. coli culture, resulting in a 19.41-fold purification with ... | 2017 | 26691486 |
| antagonistic effects of bacillus subtilis subsp. subtilis and b. amyloliquefaciens against macrophomina phaseolina: sem study of fungal changes and uv-maldi-tof ms analysis of their bioactive compounds. | the antifungal effect of bacillus subtilis subsp. subtilis pgpmori7 and bacillus amyloliquefaciens pgpbacca1 was evaluated against macrophomina phaseolina (tassi) goid. cell suspension (cs), cell-free supernatant (cfs) and the lipopeptide fraction (lf) of pgpmori7 and pgpbacca1 were screened against three different m. phaseolina strains. cs exhibited the highest inhibitory effect (around 50%) when compared to those of cfs and lf, regardless of the fungal strain studied. the synthesis of lipopept ... | 2016 | 26686611 |
| [construction and evaluation of an engineered bacterial strain for producing lipopeptide under anoxic conditions]. | biosurfactant-facilitated oil recovery is one of the most important aspects of microbial enhanced oil recovery (meor). however, the biosurfactant production by biosurfactant-producing microorganisms, most of which are aerobes, is severely suppressed due to the in-situ anoxic conditions within oil reservoirs. in this research, we successfully engineered a strain jd-3, which could grow rapidly and produce lipopeptide under anoxic conditions, by protoplast confusion using a bacillus amyloliquefacie ... | 2015 | 26685621 |
| a consortium of rhizobacterial strains and biochemical growth elicitors improve cold and drought stress tolerance in rice (oryza sativa l.). | in the present study, a consortium of two rhizobacteria bacillus amyloliquefaciens bk7 and brevibacillus laterosporus b4, termed 'bb', biochemical elicitors salicylic acid and β-aminobutyric acid (sb) and their mixture (bbsb) were investigated for cold and drought stress tolerance in rice plants. after withholding water for 16 days, rice plants treated with bbsb showed 100% survival, improved seedling height (35.4 cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting ... | 2016 | 26681628 |
| computational based functional analysis of bacillus phytases. | phytase is an enzyme which catalyzes the total hydrolysis of phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate and digests the undigestable phytate part present in seeds and grains and therefore provides digestible phosphorus, calcium and other mineral nutrients. phytases are frequently added to the feed of monogastric animals so that bioavailability of phytic acid-bound phosphate increases, ultimately enhancing the nutritional value of diets. the bacillus phytase i ... | 2016 | 26672917 |
| molecular and enzymatic characterization of alkaline lipase from bacillus amyloliquefaciens e1pa isolated from lipid-rich food waste. | bacillus amyloliquefaciens e1pa is a lipase-producing strain that was originally isolated from lipid-rich food waste, and the production of its lipase was found to be induced by vegetable oils. the e1pa lipase was successfully expressed and secreted in a heterologous escherichia coli host and was ultimately purified. the conserved pentapeptide motif ala-his-ser-met-gly was observed at positions 108-112. the purified recombinant lipase was stable over a ph range of 4.0-11.0 at 40 °c and exhibited ... | 2016 | 26672445 |
| construction of a rapid feather-degrading bacterium by overexpression of a highly efficient alkaline keratinase in its parent strain bacillus amyloliquefaciens k11. | keratinase is essential to degrade the main feather component, keratin, and is of importance for wide industrial applications. in this study, bacillus amyloliquefaciens strain k11 was found to have significant feather-degrading capacity (completely degraded whole feathers within 24 h). the keratinase encoding gene, kerk, was expressed in bacillus subtilis sck6. the purified recombinant kerk showed optimal activity at 50 °c and ph 11.0 and degraded whole feathers within 0.5 h in the presence of d ... | 2016 | 26671753 |
| selection of bacillus spp. for cellulase and xylanase production as direct-fed microbials to reduce digesta viscosity and clostridium perfringens proliferation using an in vitro digestive model in different poultry diets. | previously, our laboratory has screened and identified bacillus spp. isolates as direct-fed microbials (dfm). the purpose of the present study was to evaluate the cellulase and xylanase production of these isolates and select the most appropriate bacillus spp. candidates for dfm. furthermore, an in vitro digestive model, simulating different compartments of the gastrointestinal tract, was used to determine the effect of these selected candidates on digesta viscosity and clostridium perfringens p ... | 2015 | 26664954 |
| control of agitation and aeration rates in the production of surfactin in foam overflowing fed-batch culture with industrial fermentation. | bacillus amyloliquefaciens fmb50 produces a high yield of surfactin, a lipopeptide-type biosurfactant that has been widely studied and has potential applications in many fields. a foam overflowing culture has been successfully used in the combined production-enrichment fermentation of surfactin. in this study, the agitation and aeration rates were found to have relationships with foam formation and surfactin enrichment. a maximum surfactin concentration of 4.7g/l of foam was obtained after 21h o ... | 2016 | 26655454 |
| genome sequence of the banana plant growth-promoting rhizobacterium bacillus amyloliquefaciens bs006. | bacillus amyloliquefaciens is an important plant growth-promoting rhizobacterium (pgpr). we report the first whole-genome sequence of pgpr bacillus amyloliquefaciens evaluated in colombian banana plants. the genome sequences encode genes involved in plant growth and defense, including bacteriocins, ribosomally synthesized antibacterial peptides, in addition to genes that provide resistance to toxic compounds. | 2015 | 26607897 |
| high-quality draft genome sequence of bacillus amyloliquefaciens strain 629, an endophyte from theobroma cacao. | bacillus amyloliquefaciens strain 629 is an endophyte isolated from theobroma cacao l. here, we report the draft genome sequence (3.9 mb) of b. amyloliquefaciens strain 629 containing 16 contigs (3,903,367 bp), 3,912 coding sequences, and an average 46.5% g+c content. | 2015 | 26586881 |
| expression of essential genes for biosynthesis of antimicrobial peptides of bacillus is modulated by inactivated cells of target microorganisms. | certain bacillus strains are important producers of antimicrobial peptides with great potential for biological control. antimicrobial peptide production by bacillus amyloliquefaciens p11 was investigated in the presence of heat-inactivated cells of bacteria and fungi. b. amyloliquefaciens p11 exhibited higher antimicrobial activity in the presence of inactivated cells of staphylococcus aureus and aspergillus parasiticus compared to other conditions tested. expression of essential genes related t ... | 2016 | 26577655 |
| [degradation of lignocellulose in the corn straw by bacillus amyloliquefaciens mn-8]. | microbial degradation of lignocellulose is one of the key problems that need to be solved urgently in the process of utilizing biomass resource. bacillus amyloliquefaciens mn-8 is our previously isolated bacterium capable of degrading lignin. to determine the capability of strain mn-8 to degrade lignocellulose of corn straw, b. amyloliquefaciens mn-8 was inoculated and fermented with solid-state corn straw powder-msm culture medium. the changes in the enzyme activity and degradation products of ... | 2015 | 26571658 |
| draft genome sequence of bacillus amyloliquefaciens xk-4-1, a plant growth-promoting endophyte with antifungal activity. | here, we report the draft genome sequence of a bacterial plant-growth-promoting endophyte, bacillus amyloliquefaciens xk-4-1, which consists of one circular chromosome of 3,941,805 bp with 3,702 coding sequences (cdss). the data presented highlight multiple sets of functional genes associated with its plant-beneficial characteristics. | 2015 | 26564038 |
| diversity and antimicrobial potential of bacterial isolates associated with the soft coral alcyonium digitatum from the baltic sea. | it is well recognized that microorganisms associated with marine invertebrates, in particular sponges and hard corals, are an excellent source of new natural products. therefore, the diversity of bacteria associated with marine invertebrates and their potential to produce bioactive compounds have received much attention in recent years. we report here for the first time on the biodiversity of bacteria associated with the soft coral alcyonium digitatum, which is abundant in the baltic sea. in ord ... | 2016 | 26558794 |
| development of a model protein interaction pair as a benchmarking tool for the quantitative analysis of 2-site protein-protein interactions. | a significant challenge in the molecular interaction field is to accurately determine the stoichiometry and stepwise binding affinity constants for macromolecules having >1 binding site. the mission of the molecular interactions research group (mirg) of the association of biomolecular resource facilities (abrf) is to show how biophysical technologies are used to quantitatively characterize molecular interactions, and to educate the abrf members and scientific community on the utility and limitat ... | 2015 | 26543437 |
| exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on xiphinema index thorne & allen. | to identify enzymes and metabolites in the rhizobacteria filtrates that have a nematicidal effect on xiphinema index and perform molecular characterization of the strains evaluated. | 2016 | 26541369 |
| kinetic properties and stability of glucose dehydrogenase from bacillus amyloliquefaciens sb5 and its potential for cofactor regeneration. | glucose dehydrogenases (gludh) from bacillus species offer several advantages over other nad(p)h regeneration systems including high stability, inexpensive substrate, thermodynamically favorable reaction and flexibility to regenerate both nadh and nadph. in this research, characteristics of gludh from bacillus amyloliquefaciens sb5 (gludh-ba) was reported for the first time. despite a highly similar amino acid sequence when comparing with gludh from bacillus subtilis (gludh-bs), gludh-ba exhibit ... | 2015 | 26538191 |
| inhibition of bacillus cereus growth and toxin production by bacillus amyloliquefaciens rd7-7 in fermented soybean products. | bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has been isolated from contaminated fermented soybean food products and from the environment. b. cereus produces diarrheal and emetic toxins and has caused many outbreaks of foodborne diseases. in this study, we investigated whether b. amyloliquefaciens rd7-7, isolated from rice doenjang (korean fermented soybean paste), a traditional korean fermented soybean food, shows antimicrobial activity against b. cereus and regu ... | 2016 | 26528531 |
| evaluation of the endophytic nature of bacillus amyloliquefaciens strain gyl4 and its efficacy in the control of anthracnose. | endophytic bacteria are viewed as a potential new source of biofungicides because they have beneficial characteristics as control agents for plant disease. this study was performed to examine the endophytic feature and disease control efficacy of bacillus amyloliquefaciens strain gyl4 and to identify the antifungal compounds produced by this strain. | 2016 | 26518268 |
| antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum. | bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ... | 2015 | 26496638 |
| application of byproducts from food processing for production of 2,3-butanediol using bacillus amyloliquefaciens tul 308. | a nonpathogenic bacterial strain bacillus amyloliquefaciens tul 308 synthesized minor 2,3-butanediol (2,3-bd) amounts from glucose, fructose, sucrose, and glycerol, and efficiently produced the diol from molasses and hydrolysates of food processing residues. batch fermentations yielded 16.53, 10.72, and 5 g/l 2,3-bd from enzymatic hydrolysates of apple pomace, dried sugar beet pulp, and potato pulp (at initial concentrations equivalent to 45, 20, and 30 g/l glucose, respectively), and 25.3 g/l 2 ... | 2016 | 26460787 |
| comparison of recombinant α-hemoglobin from crocodylus siamensis expressed in different cloning vectors and their biological properties. | hemoglobin (hb) is an important component in red blood cells of the vertebrate. it is a major respiratory protein with oxygen or carbon dioxide transport function. hb has been reported to contain bioactive peptides which have antibacterial and antioxidant activities. in this study, the alpha-chain hemoglobin(αhb) gene of crocodylus siamensis was cloned into the three different expression vectors and expressed in escherichia coli bl21 (de3). the recombinant αhb proteins from all constructs could ... | 2016 | 26455814 |
| improved poly-γ-glutamic acid production in bacillus amyloliquefaciens by modular pathway engineering. | a bacillus amyloliquefaciens strain with enhanced γ-pga production was constructed by metabolically engineering its γ-pga synthesis-related metabolic networks: by-products synthesis, γ-pga degradation, glutamate precursor synthesis, γ-pga synthesis and autoinducer synthesis. the genes involved in by-products synthesis were firstly deleted from the starting nk-1 strain. the obtained nk-e7 strain with deletions of the epsa-o (responsible for extracellular polysaccharide synthesis), sac (responsibl ... | 2015 | 26410449 |
| accelerating sample preparation through enzyme-assisted microfiltration of salmonella in chicken extract. | microfiltration of chicken extracts has the potential to significantly decrease the time required to detect salmonella, as long as the extract can be efficiently filtered and the pathogenic microorganisms kept in a viable state during this process. we present conditions that enable microfiltration by adding endopeptidase from bacillus amyloliquefaciens to chicken extracts or chicken rinse, prior to microfiltration with fluid flow on both retentate and permeate sides of 0.2 μm cutoff polysulfone ... | 2016 | 26400739 |
| role of uv photolysis in accelerating the biodegradation of 2,4,6-tcp. | 2,4,6-tcp, a kind of chlorinated aromatic and aliphatic compound, is difficult to be biodegraded by ordinary microorganisms. uv photolysis and biodegradation of 2,4,6-tcp by bacillus amyloliquefaciens intimate coupling is a potential means to accelerate its biotransformation. the initial steps of 2,4,6-tcp biodegradation involve mono-oxygenation reactions that have molecular oxygen and an intracellular electron carrier as cosubstrates. it was demonstrated that b. amyloliquefaciens has the 2,4,6- ... | 2015 | 26385845 |
| purification and characterization of detergent stable alkaline protease from bacillus amyloliquefaciens sp1 isolated from apple rhizosphere. | a thermostable extracellular alkaline protease producing bacillus amyloliquefaciens sp1 was isolated from apple rhizosphere having multifarious plant growth promoting activities. strain sp1 was purified to 6.48-fold using four-step purification protocol and characterized in detail for its robustness and ecofriendly application in leather and detergent industries. structural analysis revealed that the protease was monomeric and had a molecular weight of 43 kda. it exhibited optimum activity at 60 ... | 2016 | 26375163 |
| dna-protection and antioxidant properties of fermentates from bacillus amyloliquefaciens b-1895 and bacillus subtilis katmira1933. | dna protective and antioxidant activity of bacillus amyloliquefaciens b-1895 and bacillus subtilis katmira1933 were evaluated by escherichia coli-based lux biosensors. two biosensor strains of e. coli, mg1655 (pcold-lux) and mg1655 (psoxs-lux), which react on dna damage and superoxide-anion radical activity, were used. sos-response and sox-response were stimulated by addition of dioxidine (2,3-quinoxalinedimethanol,1,4-dioxide) and paraquat (n,n'-dimethyl-4,4'-bipyridinium dichloride) respective ... | 2015 | 26370336 |
| activation of antibiotic production in bacillus spp. by cumulative drug resistance mutations. | bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. mutations in b. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction of the dipeptide antibiotic bacilysin. cumulative drug resistance mutations, such as mutations in the mtha and rpsl genes, which confer low- and high-level resistance, respectively, to streptomycin, ... | 2015 | 26369962 |
| bacillus amyloliquefaciens tsbso 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery. | a screening for biosurfactant-producing bacteria was conducted with 217 strains that were isolated from environmental samples contaminated with crude oil and/or petroleum derivatives. although 19 promising biosurfactant producers were detected, strain tsbso 3.8, which was identified by molecular methods as bacillus amyloliquefaciens, drew attention for its production of a high-activity compound that presented an emulsification activity of 63% and considerably decreased surface (28.5 mn/m) and in ... | 2015 | 26350801 |
| whole transcriptomic analysis of the plant-beneficial rhizobacterium bacillus amyloliquefaciens sqr9 during enhanced biofilm formation regulated by maize root exudates. | bacillus amyloliquefaciens sqr9 is a plant growth-promoting rhizobacteria (pgpr) with outstanding abilities to enhance plant growth and to control soil-borne diseases. root exudates is known to play important roles in plant-microbe interactions. to explore the rhizosphere interactions and plant-beneficial characteristics of sqr9, the complete genome sequence as well as the transcriptome in response to maize root exudates under biofilm-forming conditions were elucidated. | 2015 | 26346121 |
| bacillus amyloliquefaciens levansucrase-catalyzed the synthesis of fructooligosaccharides, oligolevan and levan in maple syrup-based reaction systems. | maple syrups with selected degree brix (°bx) (15, 30, 60) were investigated as reaction systems for levansucrase from bacillus amyloliquefaciens. the enzymatic conversion of sucrose present in the maple syrup and the production of the transfructosylation products were assessed over a time course of 48h. at 30°c, the use of maple syrup 30°bx led to the highest levansucrase activity (427.53μmol/mg protein/min), while maple syrup 66°bx led to the highest converted sucrose concentration (1.53m). in ... | 2015 | 26344273 |
| thermal properties of barley starch and its relation to starch characteristics. | amylopectin fine structure and starch gelatinization and retrogradation were studied in 10 different barley cultivars/breeding lines. clusters and building blocks were isolated from the amylopectin by α-amylase from bacillus amyloliquefaciens and their structure was characterized. gelatinization was studied at a starch:water ratio of 1:3, and retrogradation was studied on gelatinized starch at starch:water ratio of 1:2, by differential scanning calorimetry. three barley cultivars/breeding lines ... | 2015 | 26342462 |
| construction of a highly efficient bacillus subtilis 168 whole-cell biocatalyst and its application in the production of l-ornithine. | l-ornithine, a non-protein amino acid, is usually extracted from hydrolyzed protein as well as produced by microbial fermentation. here, we focus on a highly efficient whole-cell biocatalyst for the production of l-ornithine. the gene argi, encoding arginase, which catalyzes the hydrolysis of l-arginine to l-ornithine and urea, was cloned from bacillus amyloliquefaciens b10-127 and expressed in gras strain bacillus subtilis 168. the recombinant strain exhibited an arginase activity of 21.9 u/mg, ... | 2015 | 26314414 |
| bacillus amyloliquefaciens ag1 biosurfactant: putative receptor diversity and histopathological effects on tuta absoluta midgut. | the use of biosurfactant in pest management has received much attention for the control of plant pathogens, but few studies reported their insecticidal activity. the present study describes the insecticidal activity of biosurfactant extracted from bacillus amyloliquefaciens strain ag1. this strain produces a lipopeptide biosurfactant exhibiting an lc50 of about 180ng/cm(2) against tuta absoluta larvae. accordingly, the histopathologic effect of this biosurfactant on t. absoluta larvae showed ser ... | 2015 | 26299754 |
| complete genome sequence of bacillus amyloliquefaciens l-s60, a plant growth-promoting and antifungal bacterium. | bacillus amyloliquefaciens l-s60, a gram-positive plant-associated bacterium, which could stimulate plant growth and shows strong antifungal function, was isolated from the turfy soil in beijing, china. the genome of b. amyloliquefaciens l-s60 comprises a 3903,017bp long circular chromosome that consists of 3909 protein-coding genes and 117 rna genes. based on genomic analysis, we identified gene clusters responsible for the biosynthesis of numerous bioactive metabolites with well-established in ... | 2015 | 26297906 |
| enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in bacillus amyloliquefaciens. | bacillus amyloliquefaciens b10-127 exhibited an excellent ability for industrial-scale microbial fermentation of 2,3-butanediol (2,3-bd) from biodiesel-derived glycerol. however, the accumulation of by-products (acetoin, acetoin, lactate and succinate) and the 2,3-bd yield remains prohibitively low for commercial production. | 2015 | 26296537 |
| study of biological degradation of new poly(ether-urethane-urea)s containing cyclopeptide moiety and peg by bacillus amyloliquefaciens isolated from soil. | the present work for the first time investigates the effect of bacillus amyloliquefaciens, m3, on a new poly(ether-urethane-urea) (peuu). peuu was synthesized via reaction of 4,4'-methylenebis(4-phenylisocyanate) (mdi), l-leucine anhydride cyclopeptide (lacp) as a degradable monomer and polyethylene glycol with molecular weight of 1000 (peg-1000). biodegradation of the synthesized peuu as the only source for carbon and nitrogen for m3 was studied. the co-metabolism biodegradation of the polymer ... | 2015 | 26242387 |
| ultra high pressure homogenization (uhph) inactivation of bacillus amyloliquefaciens spores in phosphate buffered saline (pbs) and milk. | ultra high pressure homogenization (uhph) opens up new areas for dynamic high pressure assisted thermal sterilization of liquids. bacillus amyloliquefaciens spores are resistant to high isostatic pressure and temperature and were suggested as potential surrogate for high pressure thermal sterilization validation. b. amyloliquefaciens spores suspended in pbs buffer (0.01 m, ph 7.0), low fat milk (1.5%, ph 6.7), and whole milk (3.5%, ph 6.7) at initial concentration of ~10(6) cfu/ml were subjected ... | 2015 | 26236296 |
| impact of different water activities (a w) adjusted by solutes on high pressure high temperature inactivation of bacillus amyloliquefaciens spores. | much research has been conducted to comprehend the mechanisms of high pressure (hp) inactivation of spores in aqueous systems but for food model systems these information are scarce. in these systems spores can interact with ingredients which then could possibly lead to retarded or reduced inactivation, which can cause a problem for the sterilization process. the protective mechanism of a reduced a w-value is still unclear. hp processing might prove valuable to overcome protective effects of sol ... | 2015 | 26217321 |
| induction of systemic resistance in panax ginseng against phytophthora cactorum by native bacillus amyloliquefaciens hk34. | korean ginseng (panax ginseng meyer) is a perennial herb prone to various root diseases, with phytophthora cactorum being considered one of the most dreaded pathogens. p. cactorum causes foliar blight and root rot. although chemical pesticides are available for disease control, attention has been shifted to viable, eco-friendly, and cost-effective biological means such as plant growth-promoting rhizobacteria (pgpr) for control of diseases. | 2015 | 26199552 |
| characterization of an extremely salt-tolerant and thermostable phytase from bacillus amyloliquefaciens us573. | the extracellular phytase produced by the bacillus amyloliquefaciens us573 strain, isolated from geothermal soil located in southern tunisia was purified and characterized. this calcium-dependent and bile-stable enzyme (phy us573) was optimally active at ph 7.5 and 70 °c. it showed a good stability at ph ranging from 4 to 10, and especially, an exceptional thermostability as it recovered 50 and 62% of activity after heating for 10 min at 100 and 90 °c, respectively. in addition, phy us573 was fo ... | 2015 | 26188308 |
| bioactive peptides identified in thornback ray skin's gelatin hydrolysates by proteases from bacillus subtilis and bacillus amyloliquefaciens. | thornback ray skin gelatin has been hydrolyzed with two different proteases in order to obtain peptides with ace inhibitory and antioxidant activity. hydrolysates with protease from bacillus subtilis a26 (trgh-a26) displayed ace inhibitory activity with an ic50 value of 0.94 μg/μl whereas neutrase® hydrolysate from bacillus amyloliquefaciens (trgh-neutrase) showed an ic50 value of 2.07 μg/μl. regarding antioxidant activity, ic50 values of 1.98 and 21.2 μg/μl in trgh-a26 and trgh-neutrase, respec ... | 2015 | 26149667 |
| separation, determination and antifungal activity test of the products from a new bacillus amyloliquefaciens. | a new bacillus amyloliquefaciens named zju-2011 was discovered, and the culture supernatant showed a strong inhibitory effect against candida albicans. in this study, a novel method was developed to purify the antifungal compounds in high purity. the obtained products were analysed by high performance liquid chromatography and proven to be of high purity. mass spectrometry showed that the molecular weights of the two bioactive components were 270 and 288, respectively, and their structures were ... | 2016 | 26140441 |
| the global transcriptional landscape of bacillus amyloliquefaciens xh7 and high-throughput screening of strong promoters based on rna-seq data. | bacillus amyloliquefaciens is an important industrial microbe for the production of many industrial enzymes and primary metabolites. although the complete genome sequence of b. amyloliquefaciens has been now published, transcript structures of b. amyloliquefaciens remain poorly defined. in this study, high-throughput rna sequencing (rna-seq) technology was applied to dissect the transcriptome of b. amyloliquefaciens strain xh7. in total, 3936 out of a total of 4204 b. amyloliquefaciens genes (93 ... | 2015 | 26133043 |
| purification and identification of two antifungal cyclic peptides produced by bacillus amyloliquefaciens l-h15. | bacillus amyloliquefaciens l-h15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress fusarium oxysporum and other soil-borne fungal plant pathogens. two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. the two compounds were identified by tandem q-tof mass spectroscopy as c15 iturin a (1) and a novel cyclic peptide with a molecular weight of 852.4 da (2). both compounds showed good inhibitory activities ... | 2015 | 26123083 |
| cloning, enhanced expression and characterization of an α-amylase gene from a wild strain in b. subtilis wb800. | a bacillus strain with high productivity of α-amylase isolated from a starch farm was identified as bacillus amyloliquefaciens. the α-amylase encoding gene amy1 was cloned into pmd18-t vector and amplified in e. coli dh5α. shuttle vector pp43mnx was reconstructed to obtain vector pp43x for heterologous expression of the α-amylase in b. subtilis wb800. recombinant enzyme was sufficiently purified by precipitation, gel filtration and anion exchange with a specific activity of 5566 u/mg. the α-amyl ... | 2015 | 26092061 |
| comparative genomics within the bacillus genus reveal the singularities of two robust bacillus amyloliquefaciens biocontrol strains. | bacillus amyloliquefaciens cect 8237 and cect 8238, formerly known as bacillus subtilis umaf6639 and umaf6614, respectively, contribute to plant health by facing microbial pathogens or inducing the plant's defense mechanisms. we sequenced their genomes and developed a set of ad hoc scripts that allowed us to search for the features implicated in their beneficial interaction with plants. we define a core set of genes that should ideally be found in any beneficial bacillus strain, including the pr ... | 2015 | 26035127 |
| possibility of biological control of primocane fruiting raspberry disease caused by fusarium sambucinum. | biological control agents are a promising alternative to chemical pesticides for plant disease suppression. the main advantage of the natural biocontrol agents, such as antagonistic bacteria compared with chemicals, includes environmental pollution prevention and a decrease of chemical residues in fruits. this study is aimed to evaluate the impact of three bacillus strains on disease of primocane fruiting raspberry canes caused by fusarium sambucinum under controlled infection load and uncontrol ... | 2015 | 26018288 |
| structure and absolute configuration of kongiidiazadione, a new phytotoxic 3-substituted-5-diazenylcyclopentendione produced by diaporthe kongii. | a new 3-substituted-5-diazenylcyclopentendione named kongiidiazadione was isolated from culture filtrates of diaporthe kongii, associated with stem cankers on sunflower in australia. kongiidiazadione was characterized by spectroscopic (essentially nuclear magnetic resonance [nmr] and high-resolution, electrospray ionization, mass spectrometry [hresims]) methods as (-)-5-diazenyl-3-hydroxymethyl-cyclopent-3-en-1,2-dione. the stereochemistry of the diazenyl group was determined by ir spectroscopy, ... | 2015 | 26011252 |
| bacillus amyloliquefaciens supplementation alleviates immunological stress in lipopolysaccharide-challenged broilers at early age. | this study was conducted to investigate the effect of bacillus amyloliquefaciens ( ba: ) on the immune function of broilers challenged with lipopolysaccharide ( lps: ). 192 one-day-old male arbor acre broiler chickens were randomly distributed into four treatments: 1) broilers fed a basal diet; 2) broilers fed a basal diet supplemented with ba; 3) lps-challenged broilers fed a basal diet; and 4) lps-challenged broilers fed a basal diet supplemented with ba. each treatment consisted of six replic ... | 2015 | 26009750 |
| synergistic effects of [ile⁷]surfactin homologues with bacillomycin d in suppression of gray mold disease by bacillus amyloliquefaciens biocontrol strain sd-32. | we previously reported that bacillus amyloliquefaciens biocontrol strain sd-32 produces powerful antifungal lipopeptides, c17 bacillomycin d homologues. in the course of the investigation we found that the antifungal activity of the culture supernatant of this bacterium was not ascribed exclusively to bacillomycin d. we attempted to identify metabolites other than bacillomycin d to gain insight into the mechanism for the biocontrol by this bacterium. after purifying the fractions of the culture ... | 2015 | 25976169 |
| nano titania aided clustering and adhesion of beneficial bacteria to plant roots to enhance crop growth and stress management. | a novel use of titania nanoparticles as agents in the nano interface interaction between a beneficial plant growth promoting bacterium (bacillus amyloliquefaciens ucmb5113) and oilseed rape plants (brassica napus) for protection against the fungal pathogen alternaria brassicae is presented. two different tio2 nanoparticle material were produced by the sol-gel approach, one using the patented captigel method and the other one applying tibaldh precursor. the particles were characterized by transmi ... | 2015 | 25970693 |
| construction of a bacillus amyloliquefaciens strain for high purity levan production. | bacillus amyloliquefaciens nk-1 has the potential to produce levan and poly-gamma-glutamic acid (γ-pga) simultaneously. however, it is not possible to purify each single product from the same strain because the extraction process is identical. we deleted the pgs cluster (for γ-pga synthesis) from the nk-1 strain and constructed a γ-pga-deficient nk-δlp strain. nuclear magnetic results showed that the nk-δlp strain could produce high purity levan product. however, its levan titer was only 1.96 g ... | 2015 | 25953857 |
| a plasmid-born rap-phr system regulates surfactin production, sporulation and genetic competence in the heterologous host, bacillus subtilis okb105. | according to the change of environment, soil-dwelling bacillus species differentiate into distinct subpopulations, such as spores and competent cells. rap-phr systems have been found to be involved in this differentiation circuit by interacting with major regulatory proteins, such as spo0a, coma, and degu. in this study, we report that the plasmid-born rapq-phrq system found in bacillus amyloliquefaciens b3 affects three regulatory pathways in the heterologous host bacillus subtilis. expression ... | 2015 | 25921807 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| improving the quality of laminaria japonica-based diet for apostichopus japonicus through degradation of its algin content with bacillus amyloliquefaciens wb1. | laminaria japonica feedstuff is used as a substitute for sargassum thunbergii in the small-scale culturing of apostichopus japonicus (sea cucumber) because of its abundant sources and low price in china. however, the difficulty associated with the degradation of algin by a. japonicus and, hence, its utilization have limited the practical value of l. japonica feedstuff in sea cucumber farming. in this study, a. japonicus individuals were fed with l. japonica feedstuff pretreated, via fermentation ... | 2015 | 25895094 |
| draft genome sequence of bacillus amyloliquefaciens ap183 with antibacterial activity against methicillin-resistant staphylococcus aureus. | bacillus amyloliquefaciens ap183 expresses secondary metabolites that inhibit the growth of methicillin-resistant staphylococcus aureus (mrsa). here, we present a ~3.99-mbp draft genome sequence of ap183 with the aims of providing insights into the genomic basis of its antibacterial mechanisms and exploring its potential use in preventing mrsa skin colonization. | 2015 | 25883273 |
| application of a new xylanase activity from bacillus amyloliquefaciens xr44a in brewer's spent grain saccharification. | cellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. western ghat region (india) has been recognized as an active hot spot for the isolation of new microorganisms. the aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification. | 2015 | 25866429 |
| microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation. | the aim of this work was to identify and characterize the microbiota present during fermentation and in the final beverage, tarubá, by culture-dependent and -independent methods. in addition, target chemical compounds (carbohydrates, organic acids, and ethanol) were evaluated. lactic acid bacteria (lab) and mesophilic bacteria were the predominant microorganisms. among them, lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, and bacillus subtilis were frequently isolated a ... | 2015 | 25846929 |
| isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. | in this study, 100 pgpr strains isolated from different varieties of ginger (zingiber officinale rosc.) were first characterized for their morphological, biochemical, and nutrient mobilization traits in vitro. the pgpr were also screened in vitro for inhibition of pythium myriotylum causing soft rot in ginger. results revealed that only five pgpr showed >70% suppression of p. myriotylum. these 5 pgpr viz., grb (ginger rhizobacteria) 25--burkholderia cepacia, grb35--bacillus amyloliquefaciens; gr ... | 2015 | 25801969 |
| bacillus amyloliquefaciens as prophylactic treatment for clostridium difficile-associated disease in a mouse model. | probiotics might offer an attractive alternative for standard antibiotic therapy to treat clostridium difficile infections (cdi). we specifically selected a bacillus amyloliquefaciens strain for its high in vitro antibacterial activity against c. difficile and tested its efficacy to prevent cdi in a mouse model. | 2015 | 25800047 |
| characterization of a novel thermostable oligopeptidase from geobacillus thermoleovorans dsm 15325. | a gene (gt-sm3b) encoding a thermostable secreted oligoendopeptidase (gt-sm3b) was cloned from the thermophile geobacillus thermoleovorans dsm 15325. gt-sm3b is 1,857 bp in length and encodes a single-domain protein of 618 amino acids with a 23-residue signal peptide having a calculated mass of 67.7 kda after signal cleavage. the deduced amino acid sequence of gt-sm3b contains a conservative zinc metallopeptidase motif (his(400)-glu(401)-x-xhis (404)). the described oligopeptidase belongs to the ... | 2015 | 25791847 |
| plant genotype-specific archaeal and bacterial endophytes but similar bacillus antagonists colonize mediterranean olive trees. | endophytes have an intimate and often symbiotic interaction with their hosts. less is known about the composition and function of endophytes in trees. in order to evaluate our hypothesis that plant genotype and origin have a strong impact on both, endophytes of leaves from 10 olea europaea l. cultivars from the mediterranean basin growing at a single agricultural site in spain and from nine wild olive trees located in natural habitats in greece, cyprus, and on madeira island were studied. the co ... | 2015 | 25784898 |
| improvement of immunity and disease resistance in the nile tilapia, oreochromis niloticus, by dietary supplementation with bacillus amyloliquefaciens. | probiotics can be used as immunostimulants in aquaculture. the aim of this study was to evaluate the immune responses of nile tilapia oreochromis niloticus following feeding with bacillus amyloliquefaciens spores at concentrations of 1 × 10(6) (g3) and 1 × 10(4) (g2) colony-forming units per gram (cfu/g) of feed compared with a basal diet with no probiotics (g1). a total of 180 fingerlings (27.7 ± 0.22 g) were divided into three groups (g1-g3 of 20 fish per group) in triplicate. innate immunitie ... | 2015 | 25783002 |
| complete genome sequence of bacillus amyloliquefaciens strain bh072, isolated from honey. | the genome of bacillus amyloliquefaciens strain bh072, isolated from a honey sample and showing strong antimicrobial activity against plant pathogens, is 4.07 mb and harbors 3,785 coding sequences (cds). several gene clusters for nonribosomal synthesis of antimicrobial peptides and a complete gene cluster for biosynthesis of mersacidin were detected. | 2015 | 25767235 |
| bacillusin a, an antibacterial macrodiolide from bacillus amyloliquefaciens ap183. | bioassay-guided fractionation of the organic extracts of a bacillus amyloliquefaciens strain (ap183) led to the discovery of a new macrocyclic polyene antibiotic, bacillusin a (1). its structure was assigned by interpretation of nmr and ms spectroscopic data as a novel macrodiolide composed of dimeric 4-hydroxy-2-methoxy-6-alkenylbenzoic acid lactones with conjugated pentaene-hexahydroxy polyketide chains. compound 1 showed potent antibacterial activities against methicillin-resistant staphyloco ... | 2015 | 25756620 |
| characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark). | the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ... | 2015 | 25740801 |
| inactivated probiotic bacteria stimulate cellular immune responses of catla, catla catla (hamilton) in vitro. | in the present study, we investigated the in vitro immunostimulatory effects of inactivated form of two potential probiotics, bacillus amyloliquefaciens fptb16 and b. subtilis fptb13 in catla (catla catla). catla head kidney leukocytes (hk) were incubated with the bacteria alive or inactivated with heat shock (2 h at 60 °c), uv light (for 2.5 h) and formalin (1.0%, v/v) treatment (for 24 h at 4 °c) at different concentrations (10(7), 10(8) and 10(9) cells ml(-1)). after incubation, different cel ... | 2015 | 25736432 |
| whole-cell method for phenol detection based on the color reaction of phenol with 4-aminoantipyrine catalyzed by cota laccase on endospore surfaces. | a green method for phenol spectrophotometric determination was developed based on the color reaction of phenol with 4-aminoantipyrine catalyzed by addition of bacillus amyloliquefaciens endospores in the presence of o2. the catalytic activity of the endospores may be attributed to the presence of coat protein a on the cell surfaces. this deduction was confirmed by cota gene knock-out from b. amyloliquefaciens using the homologous double-exchange method. under optimal conditions, linear responses ... | 2015 | 25725465 |
| complete genome sequence of bacillus amyloliquefaciens l-h15, a plant growth promoting rhizobacteria isolated from cucumber seedling substrate. | bacillus amyloliquefaciens l-h15 is a plant growth promoting rhizobacteria (pgpr) isolated from the cucumber seedling substrate collected in beijing, china. the complete genome of b. amyloliquefaciens l-h15 consists of one single circular chromosome (3,864,316 bp) without any plasmid. from the genome, we identified clusters responsible for non-ribosomal synthesis of secondary metabolites, and genes related to the plant growth promotion hormone such as indole-3-acetic acid (iaa) and acetoin secre ... | 2015 | 25725457 |
| a novel non-blue laccase from bacillus amyloliquefaciens: secretory expression and characterization. | laccases are copper-containing enzymes which possess a promising potential in many industrial and environmental applications. here we describe the cloning, extracellular expression and characterization of a novel non-blue laccase from bacillus amyloliquefaciens in pichia pastoris. the recombinant enzyme was secreted into the culture supernatant with high activity. it lacks the absorption band at 610 nm typical for blue laccases. however, electron paramagnetic resonance (epr) spectrum proved the ... | 2015 | 25709013 |
| antagonistic mechanism of iturin a and plipastatin a from bacillus amyloliquefaciens s76-3 from wheat spikes against fusarium graminearum. | controlling toxigenic fusarium graminearum (fg) is challenging. a bacterial strain (s76-3, identified as bacillus amyloliquefaciens) that was isolated from diseased wheat spikes in the field displayed strong antifungal activity against fg. reverse-phase high performance liquid chromatography and electrospray ionization mass spectrometry analyses revealed that s76-3 produced three classes of cyclic lipopeptides including iturin, plipastatin and surfactin. each class consisted of several different ... | 2015 | 25689464 |
| the peptide toxin amylosin of bacillus amyloliquefaciens from moisture-damaged buildings is immunotoxic, induces potassium efflux from mammalian cells, and has antimicrobial activity. | amylosin, a heat-stable channel-forming non-ribosomally synthesized peptide toxin produced by strains of bacillus amyloliquefaciens isolated from moisture-damaged buildings, is shown in this paper to have immunotoxic and cytotoxic effects on human cells as well as antagonistic effects on microbes. human macrophages exposed to 50 ng of amylosin ml(-1) secreted high levels of cytokines interleukin-1β (il-1β) and il-18 within 2 h, indicating activation of the nlrp3 inflammasome, an integral part of ... | 2015 | 25681192 |
| the effects of heat activation on bacillus spore germination, with nutrients or under high pressure, with or without various germination proteins. | nutrient germination of spores of bacillus species occurs through germinant receptors (grs) in spores' inner membrane (im) in a process stimulated by sublethal heat activation. bacillus subtilis spores maximum germination rates via different grs required different 75 °c heat activation times: 15 min for l-valine germination via the gera gr and 4 h for germination with the l-asparagine-glucose-fructose-k(+) mixture via the gerb and gerk grs, with gerk requiring the most heat activation. in some c ... | 2015 | 25681191 |
| microbial growth under supercritical co2. | growth of microorganisms in environments containing co2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. thus, supercritical co2 (scco2) is generally regarded as a sterilizing agent. we report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (gcs) that are capable of growth in pressurized bioreactors containing scco2. analysis of 16s rrna genes from scco2 ... | 2015 | 25681188 |
| draft genome sequence of bacillus amyloliquefaciens jjc33m, isolated from sugarcane soils in the papaloapan region, mexico. | bacillus amyloliquefaciens strain jjc33m is a bacterium that produces α-amylase (ec 3.2.1.1) and was isolated from sugarcane soil. its estimated genome size is 3.96 mb, and it harbors 4,048 coding genes (cdss). | 2015 | 25676753 |
| in vitro evaluation of celluloytic bacillus amyloliquefaciens ams1 isolated from traditional fermented soybean (churpi) as an animal probiotic. | a microorganism showing probiotic attributes and hydrolyzing carboxymethylcellulose was isolated from traditional fermented soybean (churpi) and identified as bacillus amyloliquefaciens by analysis of 16s rrna gene sequence and named as b. amyloliquefaciens ams1. the potentiality of this isolate as probiotic was investigated in vitro and it showed gastrointestinal transit tolerance, cell surface hydrophobicity, cell aggregation and antimicrobial activity. the isolate was found to be non-hemolyti ... | 2015 | 25660401 |
| lipopeptides extract from bacillus amyloliquefaciens induce human oral squamous cancer cell death. | a lipopeptide extract of bacillus amyloliquefaciens bacy1 (ble) was found to induce cell death in human oral squamous cell carcinoma (oscc) cell lines, scc4 and scc25, in this study. the results of mtt assay showed that ble inhibited oscc cell proliferation in a dose-dependent manner. ble was also effective in increasing the sub-g1 phases. furthermore, when membrane damage in scc4 cells treated with ble was monitored by ldh assay, release of ldh was significantly increased. the protein and mrna ... | 2015 | 25640398 |
| poultry-beneficial solid-state bacillus amyloliquefaciens b-1895 fermented soybean formulation. | birds were given a new formulation of the bacillus amyloliquefaciens b-1895 solid-state fermented soybean that retained the spores of the aforementioned organism. mass dynamics, feed flow rate and broiler performance were observed to evaluate the efficacy of the formulation. at each time point, the live mass was greater than that of the control group, reaching a difference of 7-8% by day 28. a difference of 5.3-8.8% was observed in feed conversion per kilogram live mass (1.97 kg in the controls ... | 2015 | 25625034 |
| improving the neutral phytase activity from bacillus amyloliquefaciens dsm 1061 by site-directed mutagenesis. | neutral phytase is used as a feed additive for degradation of anti-nutritional phytate in aquatic feed industry. site-directed mutagenesis of bacillus amyloliquefaciens dsm 1061 phytase was performed with an aim to increase its activity. mutation residues were chosen based on multiple sequence alignments and structure analysis of neutral phytsaes from different microorganisms. the mutation sites on surface (d148e, s197e and n156e) and around the active site (d52e) of phytase were selected. analy ... | 2015 | 25613522 |
| isolation and molecular characterization of thermostable phytase from bacillus subtilis (bsphyarrmk33). | the thermostable phytase gene was isolated from bacillus subtilis arrmk33 (bsphyarrmk33). the gene has an orf of 1152 bp and that encodes a protein of 383 amino acids. sequence analysis showed high homology with bacillus sp. phytase proteins, but no similarity was found with other phytases. sds-page analysis exhibited a predicted molecular mass of 42 kda. homology modeling of bsphyarrmk33 protein based on bacillus amyloliquefaciens crystal structure disclosed its β-propeller structure. bsphyarrm ... | 2015 | 25588529 |
| utilization of different waste proteins to create a novel pgpr-containing bio-organic fertilizer. | high-quality bio-organic fertilizers (bios) cannot be produced without the addition of some proteins, while many waste proteins are haphazardly disposed, causing serious environmental pollution. in this study, several waste proteins were used as additives to assist with the reproduction of the functional microbe (bacillus amyloliquefaciens sqr9) inoculated into matured composts to produce bios. an optimized composition of solid-state fermentation (ssf) raw materials was predicted by response sur ... | 2015 | 25586328 |
| screening, gene cloning, and characterizations of an acid-stable α-amylase. | based on its α-amylase activity at ph 5.0 and optimal ph of the crude enzyme, a strain (named b-5) with acid α-amylase production was screened. the b-5 strain was identified as bacillus amyloliquefaciens through morphological, physiological, and biochemical characteristics analysis, as well as 16s rdna phylogenetic analysis. its α-amylase gene of genbank accession no. gu318401 was cloned and expressed in escherichia coli. the purified recombinant α-amylase amy-ba showed the optimal ph of 5.0, an ... | 2015 | 25563420 |
| effect of bacillus amyloliquefaciens-based direct-fed microbial on performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. | the present study was conducted to evaluate the effect of the dietary supplementation of bacillus amyloliquefaciens-based direct-fed microbial (dfm) on growth performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. a total of two hundred and eighty eight 1-d-old arbor acres male broilers were randomly allocated to one of four experimental treatments in a completely randomized design. each treatment was fed to eight replicate cages, with nine birds per c ... | 2015 | 25557820 |