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[preliminary study on genotypic diversity of streptococcus mutans in xinjiang uygur preschool children].to investigate the relationship between genotypic diversity of streptococcus mutans (sm) and deciduous teeth caries in xinjiang uygur autonomous region.201121733377
presence of cariogenic streptococci on various bracket materials detected by polymerase chain reaction.to determine the in vivo presence of streptococcus mutans and streptococcus sobrinus on different bracket materials and to correlate the prevalence with the visible plaque index (vpi) and the gingival bleeding index (gbi).201121696114
genotypic diversity of streptococcus mutans and streptococcus sobrinus in 3-4-year-old children with severe caries or without caries.international journal of paediatric dentistry 2011; 00: 000-000 background.ôçé the genotypic diversity of both streptococcus mutans and streptococcus sobrinus in children with different caries experience remains unclear. aim.ôçé to investigate the genotypic diversity of s.-ámutans and s.-ásobrinus in children with severe early childhood caries (secc) and in caries-free (cf) children. methods.ôçé stimulated saliva of 87 secc and 91 cf children aged 3-4ôçâyears was collected and submitted to culti ...201121689176
hydrophobicity test in mutans streptococci.kinetic hydrophobic measurements were performed by confronting 40 mutans streptococci from thirty 10- to 20-year-old patients with 200 ml hexadecane (sigma). fourteen patients had high dental caries risk (group a), dmft + dmft > 5 with 3 or more active caries, and 16 had low dental caries risk (group b), dmft + dmft < 3 without active caries. twenty bacteria from group a and 20 bacteria from group b were typed using de la higuera's procedure and confirmed by api strip (bio-merieux). from the 14 ...201021638961
Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification.Phytochemical investigation of a supercritical fluid extract of Glycyrrhiza uralensis has led to the isolation of 20 known isoflavonoids and coumarins, and glycycarpan (7), a new pterocarpan. The presence of two isoflavan-quinones, licoriquinone A (8) and licoriquinone B (9), in a fraction subjected to gel filtration on Sephadex LH-20 is due to suspected metal-catalyzed oxidative degradation of licoricidin (1) and licorisoflavan A (2). The major compounds in the extract, as well as 8, were evalu ...201122074222
Clinically acceptable restorations may be a hotbed for cariogenic microbes.Gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00571.x Clinically acceptable restorations may be a hotbed for cariogenic microbes Objective: The aim of this study was to investigate the cross-sectional association of dental restorations with salivary cariogenic pathogens among the elderly to establish effective parameters of caries risk for this population. Materials and methods: Stimulated whole saliva was collected from 289 community-dwelling older adults (66.2 ± 3.9 years old) who had 20 ...201122004112
The inhibition of eugenol on glucan is essential for the biofilm eradication effect on caries-related biofilm in an artificial mouth model.This study aimed to evaluate the inhibitory effects of herbal extracts on caries-related bacteria, glucan and biofilm in vitro. Sensitive tests of bacteria were carried out by broth dilution method on a 96-microwell plate. Glucan inhibition tests were carried out using the phenol sulphate method. A minimum biofilm inhibitory concentration (MBIC) test was performed in an artificial mouth model. The results of the MBIC of agents were 8, 16 and 32?mg?mL(-1) for eugenol, tannic acid and magnolol, re ...201121950685
The in vitro antimicrobial activity of natural infant fluoride-free toothpastes on oral micro-organisms.The objective of this study was to evaluate the antimicrobial activity of six toothpastes for infants: 3 fluoride-free experimental toothpastes--cashew-based, mango-based and without plant extract and fluoride compared with 2 commercially fluoride-free toothpastes and 1 fluoridated toothpastes.201122041001
Body weight maintenance and levels of mutans streptococci and lactobacilli in a group of Swedish women seven years after completion of a Weight Watchers' diet.The long-term effect of the WW programme on weight and oral cariogenic bacteria was evaluated after 7 yr. All WW who completed the 8-wk dietary regimen in an earlier study (n=33) and the persons in the reference group (REF) (n=27) were invited to participate. The salivary secretion rate, numbers of mutans streptococci (MS) and lactobacilli (lbc) were determined. The WW were weighed. Sustaining a 5% weight loss from the initial weight was regarded as successful weight maintenance. An interview ac ...201122135941
Comparative evaluation of two structurally related flavonoids, isoliquiritigenin and liquiritigenin, for their oral infection therapeutic potential.Isoliquiritigenin (1) and liquiritigenin (2) are structurally related flavonoids found in a variety of plants. The purpose of this study was to perform a comparative analysis of biological properties of these compounds in regard to their therapeutic potential for oral infections. Compound 1 demonstrated significant antibacterial activity against three major periodontopathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. In contrast, 2 exerted less pronounced ef ...201121866899
spap gene of streptococcus mutans in dental plaque and its relationship with early childhood caries.streptococcus mutans and streptococcus sobrinus are the main pathogens associated with the development of dental caries in humans. recently, the real-time polymerase chain reaction (qpcr-tr) has been used for fast and exact quantification of these bacteria species. this molecular biology method has made the detection of these bacteria in saliva and dental plaque possible; additionally, it aids the development of illness risk prediction. the purpose of this prospective, analytic, transversal, ...201122185244
Rapid detection and identification of Streptococcus ratti by a species-specific PCR method.To establish a rapid and species-specific detection and identification method of Streptococcus ratti by polymerase chain reaction, two PCR primer pairs specific to S. ratti were designed on the basis of the nucleotide sequence of the dextranase gene (dex) of S. ratti ATCC19645(T). The primer pairs specifically detected S. ratti, but none of the other mutans streptococci (16 strains of 6 species). The PCR procedure was capable of detecting 1 pg of genomic DNA purified from S. ratti ATCC19645. We ...201121924372
enterococcus faecium wb2000 inhibits biofilm formation by oral cariogenic streptococci.this study investigated the inhibitory effect of probiotic enterococcus faecium wb2000 on biofilm formation by cariogenic streptococci. the ability of e. faecium wb2000 and jcm5804 and enterococcus faecalis jcm5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. the enterococcal strains inhibited biofilm formation in dual cultures with the mutans streptococcal strains streptococcus mutans xc and streptococcus s ...201122114599
differentiation of banding patterns between streptococcus mutans and streptococcus sobrinus isolates in rep-pcr using eric primer.streptococcus mutans and streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. therefore, the establishment of a reliable genotyping method to distinguish s. mutans from s. sobrinus is of central importance.201122145074
molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization.knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique.201222196182
effects of two fluoride varnishes and one fluoride/chlorhexidine varnish on streptococcus mutans and streptococcus sobrinus biofilm formation in vitro.aims: the aim of this study was to evaluate and to compare the effect of two fluoride varnishes and one fluoride/chlorhexidine varnish on streptococcus mutans and streptococcus sobrinus biofilm formation, in vitro.study design: standard acrylic discs were prepared and divided into groups based on the varnish applied to the disc surface: fluor protector, bifluoride 12, and fluor protector + cervitec (1:1). untreated discs served as controls. in the study groups, biofilms of s. mutans and s. sobri ...201222253559
prevalence of cariogenic and periodontopathic bacteria in japanese children in the primary and mixed dentitions.the purpose of the present study was to evaluate the relationship between infection with cariogenic bacteria or periodontal pathogens and the oral condition of children in the primary and mixed dentition stages.201122900441
effects of chlorhexidine, listerine and fluoride listerine mouthrinses on four putative root-caries pathogens in the biofilm.to investigate the effects of chlorhexidine (chx), listerine and fluoride listerine on putative root-caries pathogens in the biofilm in the artificial mouth model.201122319755
lab-test(®) 4: dental caries and bacteriological analysis.dental caries is one of the most common infectious ultifactorial diseases worldwide, characterized by the progressive demineralization of the tooth, following the action of bacterial acid metabolism. the main factors predisposing the onset of the carious process are: 1) the presence of bacterial species able to lower the ph until critical values of 5.5, 2) the absence of adequate oral hygiene, 3) an inefficient immune response anti-caries, 4) the type of alimentary diet and 5) the structure of t ...201223814571
[effect of lemon essential oil on caries factors of streptococcus sobrinus].to investigate the mechanism of lemon peel essential oil (lpe) on the cariogenicity of streptococcus sobrinus (ss).201223328100
enantiomeric hplc resolution and absolute stereochemistry assignment of a new poligamain derivative.a new aryltetralin lignan derivative, 1, was obtained by reacting dimethyl succinate and piperonal, furnishing the lactone 4-(3',4'-methylenedioxybenzyl)-4,5-dihydro-2(3h)-furanone, which was reacted once again with piperonal and lda to give the dibenzylbutirolactone 7-hydroxyhinokinin. the cyclization of 7-hydroxyhinokinin into polygamain occurred in the presence of trifluoroacetic acid. the reduction of the furanic ring of polygamain was done by its reaction with dibal in thf, furnishing the d ...201223312389
microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization.to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects.201222335194
monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qpcr combined with propidium monoazide.streptococcus mutans and streptococcus sobrinus are associated with the development of dental caries in humans. however, previous diagnostic systems are unsuitable for monitoring viable cell numbers in oral specimens. assessing the relationship between the numbers of viable and dead bacterial cells and oral status is important for understanding oral infectious diseases. propidium monoazide (pma) has been reported to penetrate dead cells following membrane damage and to cross-link dna, thereby in ...201323848601
effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial.the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis.201425568808
maturation of oral microbiota in children with or without dental caries.the aim of this longitudinal study was to evaluate the oral microbiota in children from age 3 months to 3 years, and to determine the association of the presence of caries at 3 years of age.201526020247
the microbiome in populations with a low and high prevalence of caries.the oral microbiota was compared between romanian adolescents with a high prevalence of caries and no dental care and swedish caries-active and caries-free adolescents in caries prevention programs and with a low prevalence of caries. biofilm samples were analyzed by flx+ pyrosequencing of the v1 to v4 hypervariable regions of the 16s rrna gene and polymerase chain reaction (pcr)/quantitative pcr (qpcr) for streptococcus mutans and streptococcus sobrinus. sequences obtained blasted to 9 phyla, 6 ...201626442950
differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva.although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ...201627316966
comparative in vitro investigation of the cariogenic potential of bifidobacteria.this study aimed to assess the in vitro cariogenic potential of some bifidobacterium species in comparison with caries-associated bacteria.201627475723
the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study.this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ...201626949701
the effect of seasons on brazilian red propolis and its botanical source: chemical composition and antibacterial activity.the aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of brazilian red propolis (brp) and its plant source. brp was collected from maceio, alagoas state, north-east of brazil, during one year. chemical composition was determined by physicochemical analyses and hplc while antimicrobial activity was assessed against streptococcus mutans, streptococcus sobrinus, staphylococcus aureus and actinomyces naeslundii by determining the minimal i ...201727701899
[effects of yili dark bee propolis on oral cariogenic biofilm in vitro].to evaluate the effects of yili dark bee propolis on the main cariogenic biofilm and mechanisms.201526595940
[antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria].this study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. methods in this study, we investigated the antimicrobial activity of decapeptide against streptococcus mutans (s. mutans), streptococcus sobrinus, lactobacillus acidophilus, streptococcus sanguis, streptococcus gordonii, actinomyces viscosus, actinomyces naeslundii, porphyromonas gingivalis, prevotella intermedia, fusob ...201425665430
systematic screening of plant extracts from the brazilian pantanal with antimicrobial activity against bacteria with cariogenic relevance.this study proposes a bioprospection methodology regarding the antimicrobial potential of plant extracts against bacteria with cariogenic relevance. sixty extracts were obtained from ten plants--(1) jatropha weddelliana, (2) attalea phalerata, (3) buchenavia tomentosa, (4) croton doctoris, (5) mouriri elliptica, (6) mascagnia benthamiana, (7) senna aculeata, (8) unonopsis guatterioides, (9) allagoptera leucocalyx and (10) bactris glaucescens--using different extraction methods - (a) 70° ethanol ...201424603299
characterization and in vitro properties of oral lactobacilli in breastfed infants.lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ...201323945215
anti-inflammatory and antimicrobial evaluation of neovestitol and vestitol isolated from brazilian red propolis.the objective of this study was to evaluate anti-inflammatory and antimicrobial activities of neovestitol and vestitol isolated from brazilian red propolis (brp). brp ethanolic extract (eep), neovestitol, and vestitol were evaluated by anti-inflammatory properties using a neutrophil migration assay. the antimicrobial activity was evaluated by minimal inhibitory and bactericidal concentrations (mic and mbc) against streptococcus mutans , streptococcus sobrinus , staphylococcus aureus , and actino ...201323607483
antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries.silver diamine fluoride (sdf) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action.201323442825
inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria.to evaluate the antimicrobial efficacy of clearfil se protect (cp) and clearfil se bond (cb) after curing and rinsed against five individual oral microorganisms as well as a mixture of bacterial culture prepared from the selected test organisms.201424880721
screening and scoring of antimicrobial and biological activities of italian vulnerary plants against major oral pathogenic bacteria.this study aims to evaluate the activity of italian vulnerary plants against the most important oral pathogenic bacteria. this estimate was accomplished through a fivefold process: (a) a review of ethnobotanical and microbiological data concerning the italian vulnerary plants; (b) the development of a scoring system to rank the plants; (c) the comparative assessment of microbiological properties; (d) the assessment of potential cytotoxic effects on keratinocyte-like cells and gingival fibroblast ...201324302963
zoocin a and lauricidin in combination reduce streptococcus mutans growth in a multispecies biofilm.dental caries is the most prevalent human infection. it is a multifactorial disease in which the microbial composition of dental plaque plays a major role in the development of clinical symptoms. the bacteria most often implicated in the development of caries are that group of streptococci referred to as the mutans streptococci, in particular streptococcus mutans and streptococcus sobrinus. one approach to the prevention of caries is to reduce the numbers of mutans streptococci in plaque to a le ...201222508519
compositional differences in multi-species biofilms formed on various orthodontic adhesives.to investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics.201728339597
nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms.chlorhexidine (chx) is a widely used antimicrobial agent in dentistry. herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure chx (nano-chx), and its mechanical profile and antimicrobial properties against oral biofilms.201425170958
antibacterial activities of extracts from ugandan medicinal plants used for oral care.medicinal plants are widely used for treatment of oral/dental diseases in uganda.201424945400
antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms.the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ...201324077540
randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin.the aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (rmgic)], ledtb [photodynamic antimicrobial chemotherapy (pact) with light-emitting diode associated with toluidine blue solution and rmgic], and lmb ...201526502235
microbial ecosystem analysis in root canal infections refractory to endodontic treatment.the purpose of this study was to combine multiple displacement amplification and checkerboard dna-dna hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment.201627377440
streptococcus mutans, streptococcus sobrinus and candida albicans in oral samples from caries-free and caries-active children.this was to examine the occurrence of s. mutans, s. sobrinus and c. albicans in dental plaque and saliva from caries-free and caries-active greek children.201627357362
in vitro growth-inhibitory effect of ethanol gras plant and supercritical co₂ hop extracts on planktonic cultures of oral pathogenic microorganisms.conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. plants are considered as perspective sources of novel natural antiseptics. however, little is still known about their inhibitory properties against oral pathogens. the objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (gras) species against planktonic cultures of cariogenic, periodontal and candidal microorgani ...201526232134
investigations of kanuka and manuka essential oils for in vitro treatment of disease and cellular inflammation caused by infectious microorganisms.diseases caused by infectious and inflammatory microorganisms are among the most common and most severe nosocomial diseases worldwide. therefore, developing effective agents for treating these illnesses is critical. in this study, essential oils from two tea tree species, kanuka (kunzea ericoides) and manuka (leptospermum scoparium), were evaluated for use in treating diseases and inflammation caused by microorganism infection.201624582465
chitosan-thioglycolic acid as a versatile antimicrobial agent.as functionalized chitosans hold great potential for the development of effective and broad-spectrum antibiotics, representative chitosan derivatives were tested for antimicrobial activity in neutral media: trimethyl chitosan (tmc), carboxy-methyl chitosan (cmc), and chitosan-thioglycolic acid (tga; medium molecular weight: mmw-tga; low molecular weight: lmw-tga). colony forming assays indicated that lmw-tga displayed superior antimicrobial activity over the other derivatives tested: a 30 min in ...201323470196
synthesis and antimicrobial activity of geranyloxy- and farnesyloxy-acetophenone derivatives against oral pathogens.the compounds 2',6'-dihydroxy-4'-geranyloxyacetophenone (1) and 2',6'-dihydroxy-4'-farnesyloxy-acetophenone (2) are oxyprenylated secondary metabolites extracted from plants belonging to the rutaceae family. in this study, 1 and 2 were synthesized and tested for their antimicrobial activity toward major oral pathogens. compounds 1 and 2 were synthesized by selective prenylation of 2,4,6-trihydroxyacetophenone at the 4' position with geranyl and farnesyl bromide, respectively. compound 1 showed s ...201222698715
antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms.objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ...201626884763
antimicrobial activity of the essential oil of plectranthus neochilus against cariogenic bacteria.this work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves of plectranthus neochilus (pn-eo) against a representative panel of oral pathogens. we assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (mic). pn-eo displayed moderate activity against enterococcus faecalis (mic = 250 μg/ml) and streptococcus salivarus (mic = 250 μg/ml), significant activity against streptococcus sob ...201526161115
sol-gel-derived bioactive glasses demonstrate antimicrobial effects on common oral bacteria.to determine the antibacterial effect of nano-structured, sol-gel processed bioactive glasses that may be used for implants, coatings, and as adjuncts to dental restorative materials.201526087578
an agar diffusion study comparing the antimicrobial activity of nanoseal with some other endodontic sealers.the aim of this study was to evaluate the antimicrobial activity of a new experimental nano-hydroxyapatite epoxy resin-based sealer (nanoseal) with several other commercially available sealers; ah26, tubliseal, sealapex and roekoseal against enterococcus faecalis, pseudomonas aeruginosa, streptococcus mutans, streptococcus sobrinus and escherichia coli for up to 7 days. agar diffusion was used in this study. fifty muller-hinton agar plates were prepared and divided into five experimental groups ...201222827817
application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation.water-insoluble glucan (wig) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze wig. the purposes of this study were to construct bi-functional chimeric glucanase, composed of mutanase and dextranase, and to examine the effects of this chimeric glucanase on the formation ...201525411090
functional ginger extracts from supercritical fluid carbon dioxide extraction via in vitro and in vivo assays: antioxidation, antimicroorganism, and mice xenografts models.supercritical fluid carbon dioxide extraction technology was developed to gain the active components from a taiwan native plant, zingiber officinale (ginger). we studied the biological effects of ginger extracts via multiple assays and demonstrated the biofunctions in each platform. investigations of ginger extracts indicated antioxidative properties in dose-dependant manners on radical scavenging activities, reducing powers and metal chelating powers. we found that ginger extracts processed mod ...201323983624
inhibition of initial adhesion of oral bacteria through a lectin from bauhinia variegata l. var. variegata expressed in escherichia coli.the aim of the present work was to study the in vitro effect of native and recombinant bauhinia variegata var. variegata lectins in inhibiting early adhesion of streptococcus mutans, streptococcus sanguis and streptococcus sobrinus to experimentally acquired pellicle.201323910219
comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care.probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ...201627999400
inhibitory effects of arabitol on caries-associated microbiologic parameters of oral streptococci and lactobacilli.the aim of this study was to compare arabitol with its better studied isomer xylitol for their inhibitory effects on cell growth and acid production of oral bacteria. streptococcus mutans, streptococcus salivarius and streptococcus sobrinus were used as representatives of oral streptococci and lactobacillus acidophilus and lactobacillus fermentum were used for oral lactobacilli. growth was followed by measuring the absorbance at 660nm, acid production by ph change. sensitivity of these oral bact ...201526433188
effects of baseplates of orthodontic appliances with in situ generated silver nanoparticles on cariogenic bacteria: a randomized, double-blind cross-over clinical trial.polymethyl-methacrylate (pmma) is commonly used primarily for baseplates of orthodontic appliances (boa). the activities of cariogenic bacteria in biofilm on these surfaces may contribute to dental caries, gingival inflammation and periodontal disease. the pmma incorporated with nanoparticles of silver (nanoag-i-pmma) and nanoag in situ in pmma (nanoag-is-pmma) have been shown to control the growth of cariogenic bacteria, but clinical trial of anti-cariogenic application of these novel materials ...201526067732
development of novel formulations containing lysozyme and lactoferrin and evaluation of antibacterial effects on mutans streptococci and lactobacilli.the aim of this study was to determine the antibacterial effect of different formulations containing lysozyme and lactoferrin and drug delivery system as well as poloxamer 407 with the trade name of pluronic f-127 and/or freeze dried liposome containing dotap [freeze dried liposomal dotap] on streptococcus sobrinus, streptococcus mutans and lactobacillus acidophilus in comparison with 0.2% chlorhexidine.201525757148
antimicrobial activity of fractions and subfractions of elaeagia utilis against microorganisms of importance in dental caries.dental caries is a multifactorial infectious disease that leads to the destruction of dental hard tissue. the main goal of research into medicinal plants is to seek compounds with antimicrobial activity for subsequent use in prevention strategies and control of infectious diseases. the aim of this study was to evaluate the antimicrobial activity of fractions and subfractions obtained from elaeagia utilis against streptococcus mutans, streptococcus sobrinus and lactobacillus acidophilus. the plan ...201324303734
analysis of cariogenic bacteria in saliva of cancer patients.this study examined salivary flow and salivary ph and the prevalence and levels of cariogenic bacteria in the saliva of oncological patients and healthy controls. quantitative real-time polymerase chain reaction was used to assess the levels of microbes including streptococcus mutans, streptococcus sobrinus, lactobacillus salivarius, and lactobacillus acidophilus in the saliva of 41 patients with a solid tumor (so), 30 patients with a hematologic malignancy (he), and 40 healthy controls. salivar ...201324010070
effects of 2 bracket and ligation types on plaque retention: a quantitative microbiologic analysis with real-time polymerase chain reaction.the aim of this study was to evaluate the effects of self-ligating brackets and conventional brackets ligated with stainless steel ligatures on dental plaque retention and microbial flora.201323910207
composition and activity against oral pathogens of the essential oil of melampodium divaricatum (rich.) dc.the chemical composition of the essential oil isolated from the aerial parts of melampodium divaricatum (rich.) dc. (asteraceae) was characterized by gc-fid and gc/ms analyses. (e)-caryophyllene (56.0%), germacrene d (12.7%), and bicyclogermacrene (9.2%) were identified as the major oil components. the antimicrobial activity of the oil against seven standard strains of oral pathogens from the american type culture collection (atcc) was evaluated by determining minimum inhibitory concentrations ( ...201424634073
salivary iga response to probiotic bacteria and mutans streptococci after the use of chewing gum containing lactobacillus reuteri.we investigated whether ingestion of probiotic bacteria could influence salivary iga levels, specific anti-mutans streptococci iga levels and specific antibodies towards the ingested probiotic bacterium. the study was a randomised, double-blind, placebo-controlled trial, where the test group (n = 11) received twice daily chewing of gum containing lactobacillus reuteri (2 × 10(8)  cfu per dose) and the control group (n = 12) received placebo. resting saliva was collected before and after 12 weeks ...201323737255
synthesis and screening of n-acyl thiolated chitosans for antibacterial applications.low-molecular weight chitosan-thioglycolic acid has shown significant antibacterial properties against different microorganisms. in order to explore the potential and structure-activity relationships of newly synthesized alkyl thiomers, chitosan has been functionalized with a series of thio-acids with increasing alkyl chain length. all thiomers were characterized with special emphasis on the determination of their degree of deacetylation and substitution, as well as on their molecular weight and ...201627474669
pcr-dipstick dna chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets.the onset of plaque-mediated disease, including dental caries and periodontal diseases, is highly associated with compositional change of the resident microflora from the ecological perspective. as specific bacterial profiles have been linked to different disease stages, microbial compositional measurements might therefore have great value for clinical diagnosis. previously we have reported a dry-reagent strip biosensor-pcr-dipstick dna chromatography, which utilized molecular recognition of oli ...201626912138
rapid and sensitive pcr-dipstick dna chromatography for multiplex analysis of the oral microbiota.a complex of species has been associated with dental caries under the ecological hypothesis. this study aimed to develop a rapid, sensitive pcr-dipstick dna chromatography assay that could be read by eye for multiplex and semiquantitative analysis of plaque bacteria. parallel oligonucleotides were immobilized on a dipstick strip for multiplex analysis of target dna sequences of the caries-associated bacteria, streptococcus mutans, streptococcus sobrinus, scardovia wiggsiae, actinomyces species, ...201425485279
distribution of streptococcus troglodytae and streptococcus dentirousetti in chimpanzee oral cavities.the aim of this study was to analyze the distribution and phenotypic properties of the indigenous streptococci in chimpanzee (pan troglodytes) oral cavities. eleven chimpanzees (aged from 9 to 44 years, mean ± sd, 26.9 ± 12.6 years) in the primate research institute of kyoto university were enrolled in this research and brushing bacterial samples collected from them. streptococci were isolated from the oral cavities of all chimpanzees. the isolates (n = 46) were identified as thirteen species by ...201323668608
gbpc gene repertoire variation among mutans streptococci.the human dental caries pathogen streptococcus mutans harbors one glucan-binding wall-anchored protein gene, gbpc, and another human pathogen, streptococcus sobrinus, has 4 gbpc homologues. as no 4 gbpc homologues have so far been detected in other mutans streptococci, however, we cannot say whether those in s. sobrinus are paralogous or orthologous. therefore, the purpose of this study was to identify and sequence the gbpc/dbl genes in streptococcus downei and streptococcus criceti. the finding ...201222790333
factors related to maternal transmission of mutans streptococci in high-risk children-pilot study.the purpose of this study was to investigate virulence factors associated with maternal transmission of mutans streptococci (ms).201223014079
comparison of oral streptococci biofilm in caries-free and caries-affected preschool mexican children.interaction of oral streptococci biofilm is the main etiological factor for dental caries. the aim of the study was to compare oral streptococci (os) distribution in the biofilm of primary dentition from caries-free and caries-affected preschool mexican children. this cross-sectional study involved 40 caries-free and 40 caries-affected children with primary dentition. each child was examined using the dmfs index, dna was extracted from saliva and presence of os was determined by pcr. data obtain ...201222928378
[influence of surface roughness on oral streptococcal adhesion forces to dental filling materials].this study is to determine the common oral streptococcal adhesion forces by using composite resin and glass ionomer cement (gic) with different degrees of surface roughness via atomic force microscopy (afm) analysis. the influence of surface roughness on bacterial adhesion force is also discussed.201628326699
effect of enamel morphology on nanoscale adhesion forces of streptococcal bacteria : an afm study.we explore the influence of enamel surface morphology on nanoscale bacterial adhesion forces. three dimensional morphology characteristics of enamel slices, which were treated with phosphoric acid (for 0 s, 5 s, 10 s, 20 s, and 30 s), were acquired. adhesion forces of three initial colonizers (streptococcus oralis, streptococcus sanguinis, and streptococcus mitis) and two cariogenic bacterial strains (streptococcus mutans and streptococcus sobrinus) with etched enamel surfaces were determined. c ...201526482011
evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of aloysia citriodora (lemon verbena) on streptococcus mutans and streptococcus sobrinus.the aloysia citriodora plant from the family of verbenaceae has many uses in traditional medicine. the aim of the current study was to determine the effects of the aqueous and ethanolic extracts of a. citriodora on streptococcus mutans and streptococcus sobrinus, which cause tooth decay.201628163849
distribution of streptococcus mutans and streptococcus sobrinus in dental plaque of indian pre-school children using pcr and sb-20m agar medium.dental caries is one of the most common infectious diseases affecting the oral cavity. among the oral bacteria, mutans streptococci have been implicated as major cariogenic bacteria as they can produce high levels of dental caries causing substances such as lactic acid and extracellular polysaccharides.201628050506
five-year longitudinal study of dental caries risk associated with streptococcus mutans and streptococcus sobrinus in individuals with intellectual disabilities.streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) are important etiologic agents in human dental caries. using quantitative real-time polymerase chain reaction assays for the presence of those strains, we examined 145 outpatients with intellectual disability (id), calculated the proportion of each of these strains to total bacteria, and compared dental caries incidence over 5 years. plaque samples were collected from all erupted tooth sites, and dental examinations were p ...201728049966
no evidence for the growth-stimulating effect of monomers on cariogenic streptococci.in spite of contradicting results, the high susceptibility of composites for secondary caries is still often associated with the bacterial growth-stimulating effect of released methacrylate monomers. however, most studies that showed this effect were performed with techniques having inherent limitations (spectrophotometry).201627766489
streptococcusdentiloxodontae sp. nov., isolated from the oral cavity of elephants.a gram-stain-positive, catalase-negative, coccus-shaped organism was isolated from oral cavity samples collected from healthy elephants. the isolated strain, num 2404t, was tentatively identified as a streptococcal species based on the results of biochemical tests. although a comparative 16s rrna gene sequence analysis suggested the classification of this organism into the genus streptococcus, it did not correspond to any recognized species of the genus. strain num 2404t was related most closely ...201627381035
pleurocidin congeners demonstrate activity against streptococcus and low toxicity on gingival fibroblasts.fish epidermal antimicrobial peptides, such as pleurocidin, are cathelicidins with broad-spectrum antimicrobial activity against gram negative and gram-positive bacteria, as well as fungi. in the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities.201627341459
influence of sucrose and xylitol on an early streptococcus mutans biofilm in a dental simulator.in vitro methods to study dental biofilms are useful in finding ways to support a healthy microbial balance in the oral cavity. the effects of sucrose, xylitol, and their combination on three strains of streptococcus mutans and one strain of streptococcus sobrinus were studied using a dental simulator.201627318453
evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: an in vitro study.the most prevalent and worldwide oral disease is dental caries that affects a significant proportion of the world population. there are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. therefore new methods are needed for better management of this important challenge. chitosan is a natural and non-toxic polysaccharide with many biological applications, particularly as an antimicrobial agent. ch ...201627307974
frequency of dental caries in active and inactive systemic lupus erythematous patients: salivary and bacterial factors.the objective of this study was to determine dental caries frequency and to analyze salivary and bacterial factors associated with active and inactive systemic lupus erythematous (sle) patients. also, a proposal to identify dental caries by a surface, teeth, and the patient was developed.201627053402
cariogram outcome after 90 days of oral treatment with streptococcus salivarius m18 in children at high risk for dental caries: results of a randomized, controlled study.dental caries is the most common chronic disease of childhood. cariogram is a well-recognized algorithm-based software program based on different caries-related risk factors and intended to aid clinicians in performing more objective and consistent dental caries risk assessments. this type of approach precedes the diagnosis of caries and allows the dentist to identify at-risk patients and then take appropriate preventive measures before caries develop further. one of the etiological factors favo ...201526491371
longitudinal study of dental caries incidence associated with streptococcus mutans and streptococcus sobrinus in patients with intellectual disabilities.mutans streptococci (streptococcus mutans and s. sobrinus) are considered to be major etiologic agents of dental caries. using a polymerase chain reaction method, we detected those bacteria from 145 outpatients (6-30 years old) with intellectual disabilities (id) and their presence was compared with the incidence of dental caries.201526328921
in vitro antibacterial activity of nano silver ion substituted poly acrylic acid films on titanium by plasma polymerization.antibacterial activity of oral pathogens such as streptococcus mutans, streptococcus sobrinus when silver ion immobilized on commercially pure (cp) titanium (ti) surface was investigated in this study. plasma-polymerized acrylic acid to have carboxyl group was deposited on cp-ti surface and then ion-exchanged with ag+ ions in 0.1 n agno3. in anti-adherent experiment, antibacterial activity was tested using broth culture methods. the biofilm formation assay was performed using semi-defined biofil ...201526328382
oral streptococci biofilm formation on different implant surface topographies.the establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. this study evaluated the biofilm formation capacity of five oral streptococci species on two titanium surface topographies. in vitro biofilm formation was induced on 30 titanium discs divided in two groups: sandblasted acid-etched (sae- n = ...201526273590
streptococcus mutans and streptococcus sobrinus colonization and caries experience in 3- and 5-year-old thai children.the aim of this study was to examine the colonization of streptococcus mutans and streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ecc) in thai children.201525753978
the corrosion resistance of wiron(®)88 in the presence of s. mutans and s. sobrinus bacteria.the corrosion resistance of wiron(®)88, a ni-cr-mo alloy, was evaluated in liquid growth media in the absence and presence of the streptococcus sobrinus and streptococcus mutans strains. open circuit potential measurements, cyclic voltammetry, linear sweep voltammetry, as well as electronic microscopy coupled to electron diffraction spectroscopy (sem/eds), were the main techniques used in this study. it was concluded that the presence of s. sobrinus and s. mutans have only a slight effect on the ...201525578707
comparing the cariogenic species streptococcus sobrinus and s. mutans on whole genome level.two closely related species of mutans streptococci, namely streptococcus mutans and streptococcus sobrinus, are associated with dental caries in humans. their acidogenic and aciduric capacity is directly associated with the cariogenic potential of these bacteria. to survive acidic and temporarily harsh conditions in the human oral cavity with hundreds of other microbial co-colonizers as competitors, both species have developed numerous mechanisms for adaptation.201425475081
synergistic inhibition of streptococcal biofilm by ribose and xylitol.streptococcus mutans and streptococcus sobrinus are the major causative agents of human dental caries. therefore, the removal or inhibition of these streptococcal biofilms is essential for dental caries prevention. in the present study, we evaluated the effects of ribose treatment alone or in combination with xylitol on streptococcal biofilm formation for both species. furthermore, we examined the expression of genes responsible for dextran-dependent aggregation (ddag). in addition, we investiga ...201525463908
synergistic effect of xylitol and ursolic acid combination on oral biofilms.this study was designed to evaluate the synergistic antibacterial effect of xylitol and ursolic acid (ua) against oral biofilms in vitro.201425383348
reducing dental plaque formation and caries development. a review of current methods and implications for novel pharmaceuticals.dental caries is an oral disease, which has a high worldwide prevalence despite the availability of various prophylactic means, including the daily use of fluoride toothpastes, water fluoridation, dental sealants, oral health educational programs and various antiseptic mouth-rinses. one important reason for this is uncontrolled increase in consumption of foods containing considerable sucrose concentration, especially among children. sucrose is easily metabolized by oral bacteria (mostly streptoc ...201425209226
characterization and bacterial anti-adherent effect on modified pmma denture acrylic resin containing platinum nanoparticles.this study characterized the synthesis of a modified pmma (polymethyl methacrylate) denture acrylic loading platinum nanoparticles (ptn) and assessed its bacterial inhibitory efficacy to produce novel antimicrobial denture base material.201425006385
[analysis of caries susceptibility factors during transition from caries-free to caries in three-year-old children].to investigate the changes of caries susceptibility factors during the transition from caries-free to caries in three-year-old children.201424969591
mutans streptococci enumeration and genotype selection using different bacitracin-containing media.the primary etiological agents associated with dental caries include the mutans streptococci (ms) comprised of streptococcus mutans and streptococcus sobrinus. the effective cultivation and isolation of ms are necessary for the study of ms, including their proper clinical assessment in the epidemiological study of dental caries. several selective media have been developed for the isolation, enumeration, and characterization of ms. however, inhibition of ms may occur, reducing counts and perhaps ...201424878141
inhibitory effect of ti-ag alloy on artificial biofilm formation.titanium-silver (ti-ag) alloy has been improved for machinability and mechanical properties, but its anti-biofilm properties have not been elucidated yet. thus, this study aimed to evaluate the effects of ti-ag alloy on biofilm formation and bacterial viability in comparison with pure ti, pure ag and silver-palladium (ag-pd) alloy. biofilm formation on the metal plates was evaluated by growing streptococcus mutans and streptococcus sobrinus in the presence of metal plates. bactericidal activity ...201424786344
quantitative analysis of changes in salivary mutans streptococci after orthodontic treatment.the purpose of this study was to analyze the initial changes in salivary mutans streptococci levels after orthodontic treatment with fixed appliances.201424785924
comparative short-term in vitro analysis of mutans streptococci adhesion on esthetic, nickel-titanium, and stainless-steel arch wires.to test the hypothesis that there are no differences in mutans streptococci (ms) adhesion between esthetic and metallic orthodontic arch wires based on their surface characteristics.201424308530
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