| [structural and hormonal alterations in the pancreas after hepatectona in dogs]. | humoral factors in the portal blood have often been suggested to play an important role in hepatic regeneration after hepatectomy. however, the correlation among insulin, glucagon and somatostatin, and islet cells of langerhans has not been studied. forty percent hepatectomy was carried out in dogs and intravenous glucose administration (0.5 g/kg) was the function of the pancreas to release one week after and one month after surgery to estimate administered intravenously before surgery, the plas ... | 1986 | 2870427 |
| [structural and hormonal alterations in the pancreas after hepatectona in dogs]. | humoral factors in the portal blood have often been suggested to play an important role in hepatic regeneration after hepatectomy. however, the correlation among insulin, glucagon and somatostatin, and islet cells of langerhans has not been studied. forty percent hepatectomy was carried out in dogs and intravenous glucose administration (0.5 g/kg) was the function of the pancreas to release one week after and one month after surgery to estimate administered intravenously before surgery, the plas ... | 1986 | 2870427 |
| the influence of beta-agonists on normal and clonidine-decelerated noradrenaline turnover in the rat brain. | the effect of some beta-adrenoceptor agonists, such as clenbuterol, salbutamol, isoprenaline and formoterol on the na turnover (estimated on the basis of na disappearance after inhibition of its synthesis by fla-63) in various regions of the rat brain was examined. all the used beta-agonists (given ip) accelerated the fla-63 induced disappearance of na in the cortex, limbic system and brain stem. that effect of the beta-agonists was antagonized by propranolol. the obtained results indicate that ... | 1985 | 2870484 |
| [experimental determination of the time-dependent extent of after-burning with reference to possibilities of the plastic surgery reconstruction of 3d degree burns]. | the present experiments try to answer the question as to the time-dependent extent of the after-burning process after full-thickness burn (third degree). for an early plastic surgical treatment it was of interest to determine the most early time of escharotomy. the time-dependent spreading of the after-burning area reached its maximum five days after the burn injury. the after-burning area was marked by intravenous injections of patentblau which caused distinct intravital colouring. subsequently ... | 1986 | 2870620 |
| [experimental determination of the time-dependent extent of after-burning with reference to possibilities of the plastic surgery reconstruction of 3d degree burns]. | the present experiments try to answer the question as to the time-dependent extent of the after-burning process after full-thickness burn (third degree). for an early plastic surgical treatment it was of interest to determine the most early time of escharotomy. the time-dependent spreading of the after-burning area reached its maximum five days after the burn injury. the after-burning area was marked by intravenous injections of patentblau which caused distinct intravital colouring. subsequently ... | 1986 | 2870620 |
| effect of sympathetic blockade on central prostaglandin e2-induced hyperthermia. | the mechanisms by which intracerebroventricular (i.v.t.) prostaglandin e2 (pge2) produce hyperthermia in the rat were investigated. i.v.t. pge2 produced dose-related increases in blood pressure, heart rate and rectal temperature which were significant with a dose of 0.5 ng. oxygen consumption also increased and remained above baseline over an hour with 50 and 500 ng pge2 doses. ganglionic blockade with hexamethonium (20 mg/kg) attenuated the blood pressure and heart rate response to pge2 but met ... | 1986 | 2870771 |
| effect of sympathetic blockade on central prostaglandin e2-induced hyperthermia. | the mechanisms by which intracerebroventricular (i.v.t.) prostaglandin e2 (pge2) produce hyperthermia in the rat were investigated. i.v.t. pge2 produced dose-related increases in blood pressure, heart rate and rectal temperature which were significant with a dose of 0.5 ng. oxygen consumption also increased and remained above baseline over an hour with 50 and 500 ng pge2 doses. ganglionic blockade with hexamethonium (20 mg/kg) attenuated the blood pressure and heart rate response to pge2 but met ... | 1986 | 2870771 |
| enkephalin-containing polypeptides in human cerebrospinal fluid. | the chemical nature of peptides in human csf with the enkephalin core sequence from proenkephalin a and proenkephalin b, was investigated. direct measurements with radioimmunoassay (ria) were run on enkephalin, enkephalin hexapeptides, dynorphin a, dynorphin a1-8 and dynorphin b. the hexapeptides occurred in about 3 times higher concentration than the corresponding enkephalins. the only dynorphin ria which gave positive results was the one for dynorphin a. however, most dynorphin a immunoactive ... | 1986 | 2870778 |
| steady state kinetics of 14c-sulfamethazine [4-amino-n-(4,6-dimethyl-2-pyrimidinyl)benzene[u-14c]sulfonamide] metabolism in swine. | swine were dosed orally with 14c-sulfamethazine [4-amino-n-(4, 6-dimethyl-2-pyrimidinyl)benzene[u-14c]sulfonamide] for 3, 5, or 7 days (two 165-mg doses/day; 0.46 muci/mg) and killed 8 hr after the last dose. the concentration of carbon-14 in the tissues increased by an average of 21% from day 3 to day 5 of dosing. however, there was no further increase from day 5 to day 7, indicating that a steady state level of carbon-14 in the tissues was attained by dosing on 5 consecutive days. liver, kidne ... | 1986 | 2870888 |
| steady state kinetics of 14c-sulfamethazine [4-amino-n-(4,6-dimethyl-2-pyrimidinyl)benzene[u-14c]sulfonamide] metabolism in swine. | swine were dosed orally with 14c-sulfamethazine [4-amino-n-(4, 6-dimethyl-2-pyrimidinyl)benzene[u-14c]sulfonamide] for 3, 5, or 7 days (two 165-mg doses/day; 0.46 muci/mg) and killed 8 hr after the last dose. the concentration of carbon-14 in the tissues increased by an average of 21% from day 3 to day 5 of dosing. however, there was no further increase from day 5 to day 7, indicating that a steady state level of carbon-14 in the tissues was attained by dosing on 5 consecutive days. liver, kidne ... | 1986 | 2870888 |
| metabolism of 4,4'-methylenebis(2-chloroaniline) by canine liver and kidney slices. | 4,4'-methylenebis(2-chloroaniline) (mboca) metabolism in canine liver and kidney slices was investigated using hplc to separate the metabolites. liver slices metabolized 5-10% of the 14c-mboca in 60 min and produced seven metabolites resolved by hplc. the major metabolite, representing approximately 80% of the metabolism, was 2-amino-5-[(4-amino-3-chlorophenyl)methyl]-3-chlorophenyl hydrogen sulfate, previously identified as the major urinary metabolite in dogs. an mboca-glucoside was identified ... | 1986 | 2870890 |
| metabolism of 4,4'-methylenebis(2-chloroaniline) by canine liver and kidney slices. | 4,4'-methylenebis(2-chloroaniline) (mboca) metabolism in canine liver and kidney slices was investigated using hplc to separate the metabolites. liver slices metabolized 5-10% of the 14c-mboca in 60 min and produced seven metabolites resolved by hplc. the major metabolite, representing approximately 80% of the metabolism, was 2-amino-5-[(4-amino-3-chlorophenyl)methyl]-3-chlorophenyl hydrogen sulfate, previously identified as the major urinary metabolite in dogs. an mboca-glucoside was identified ... | 1986 | 2870890 |
| hepatic and muscle clearance of physostigmine in the rat. | physostigmine (phy) was rapidly metabolized by rat liver after iv (100 micrograms/kg) or im (650 micrograms/kg) administration. the half-life of phy in liver after iv and im administration was found to be 24 and 26 min, respectively, indicating that phy does not show dose-dependent kinetics. the half-life of phy in muscle after iv administration was 20 min. the extraction ratio and the clearance from the liver and muscle were calculated. hepatic clearance ws found to be 23.08 ml/min/kg (34.90% o ... | 1986 | 2870892 |
| hepatic and muscle clearance of physostigmine in the rat. | physostigmine (phy) was rapidly metabolized by rat liver after iv (100 micrograms/kg) or im (650 micrograms/kg) administration. the half-life of phy in liver after iv and im administration was found to be 24 and 26 min, respectively, indicating that phy does not show dose-dependent kinetics. the half-life of phy in muscle after iv administration was 20 min. the extraction ratio and the clearance from the liver and muscle were calculated. hepatic clearance ws found to be 23.08 ml/min/kg (34.90% o ... | 1986 | 2870892 |
| transovarial transmission of tahyna virus by aedes aegypti mosquitoes (strain bangkok) | | 1986 | 2873736 |
| the neurobiologic consequences of down syndrome. | trisomy of the whole or distal part of human chromosome 21 (hsa 21) (ts21) results in down syndrome (ds), which is characterized in part by mental retardation and associated neurological abnormalities. structural abnormalities observed frequently include reduced brain weight, decreased number and depth of sulci in the cerebral cortices, neuronal heterotopias, and reduced numbers of specific populations of neurons, such as granule cells, in the cerebral cortices. abnormalities in the structure of ... | 1986 | 2875770 |
| the fine structure of atypical ciliated cells in the human gastric epithelium. | gastric mucosa obtained from the body and pyloric portions of the human stomach were observed by light and transmission electron microscopy. ciliated cells were found in two of 18 subjects examined, one patient with gastric ulcer and the other one with gastric adenocarcinoma. the ciliated cells were found in epithelia at sites away from the main lesions. the tissues containing ciliated cells showed intestinal metaplasia combined with mild chronic gastritis in both cases. the epithelial layer fac ... | 1986 | 2876543 |
| [human pathogenic papillomaviruses and cancer]. | | 1986 | 2877377 |
| human papillomavirus dna in bronchial squamous cell carcinomas. | | 1987 | 2880009 |
| onset and duration of immunity in guinea pigs and mice induced with different q fever vaccines. | protective effects of different types of q fever vaccines, namely untreated coxiella burnetii phase i cells (cb i) or cb i cells treated with chloroform-methanol (cm) mixture (cb i-cm) and of a q fever chemovaccine obtained by trichloroacetic acid extraction (tcae) from intact cb i cells, were compared in mice and guinea pigs at different intervals after intraperitoneal (i.p.) or subcutaneous (s.c.) immunizations. the highest degree of protection at all intervals studied was achieved with cb i c ... | 1986 | 2881470 |
| experimental double infection with coxiella burnetii and tick-borne encephalitis virus in dermacentor reticulatus ticks. | experimental parenteral inoculation of dermacentor reticulatus ticks with the rickettsia coxiella burnetii (c.b.) and tick-borne encephalitis (tbe) virus resulted in a generalized rickettsial and viral infection, irrespective of whether the agents were given simultaneously or by a 7 days interval apart. both agents multiplied intensively in ticks, c.b. being detectable predominantly in cytoplasmic vacuoles and tbe virus mostly in the endoplasmic reticulum. | 1987 | 2883860 |
| antigenicity of chloroform-methanol-treated coxiella burnetii preparations. | phase i coxiella burnetii (c.b.) cells untreated (cb i) or treated with chloroform-methanol (cm) mixture (cb i-cm) were compared as to their capacity to induce antibodies in laboratory animals and cattle, their ability to elicit delayed type hypersensitivity (dth) reaction in mice and rabbits and protective effect in mice. in all animal species (mice, guinea pigs, rabbits, cattle) tested, the same doses of cb i-cm cells induced lower levels of both phase i and phase ii microagglutinating (ma) an ... | 1987 | 2886025 |
| the formation of glutathione conjugate derived from propranolol. | mixed function oxidases in liver microsomes from 3-methylcholanthrene-pretreated guinea pigs converted s-propranolol to 4-hydroxypropranolol, 5-hydroxypropranolol, and desisopropyl-propranolol. the addition of glutathione and cytosol from rat liver to the system resulted in the formation of a water-soluble metabolite. evidence that the metabolite was a glutathione adduct was obtained by showing that treatment with gamma-glutamyltranspeptidase changed its hplc retention time. the formation of the ... | 1987 | 2886310 |
| composite effects of actinonin when inhibiting enkephalin-degrading enzymes. | actinonin which has been found to be an inhibitor of aminopeptidase m (ec 3.4.11.2) also inhibited enkephalinase a (ec 3.4.24.11) and enkephalin aminopeptidase which were partially purified from the corpus striatum membrane of guinea-pig brain. the ic50 values were 5.6 microm for enkephalinase a and 0.39 microm for enkephalin aminopeptidase. actinonin also inhibited with an ic50 value of 1.1 microm dipeptidyl aminopeptidase tested on whole brain homogenate of rats in the presence of thiorphan an ... | 1987 | 2886348 |
| coxiella burnetii antigens for detection of q fever antibodies by elisa in human sera. | different concentrations of chemically-treated (by potassium periodate oxidation or mild acid hydrolysis) purified phase i coxiella burnetii (c.b.) corpuscles and natural (untreated) purified phase i and phase ii c.b. corpuscles were compared by elisa for detection of both phase i (directed to antigen 1) and phase ii (directed to antigen 2) antibodies in human q fever convalescent sera. as to the absorbance values the most sensitive was the antigen obtained by mild acid hydrolysis (0.1 mol/l hcl ... | 1987 | 2888290 |
| antibiotic dependence in a strain of neisseria pharyngis. | a strain of neisseria pharyngis was isolated in pure culture from a specimen of sputum. growth was markedly enhanced by sub-lethal concentrations of rifampicin, spectinomycin, erythromycin and clindamycin. the effect was temporary with tetracycline. the possible mechanisms of this phenomenon are discussed. | 1987 | 2888818 |
| prevalence of antibodies to human t-cell leukemia/lymphoma virus type i and human immunodeficiency virus in japanese immigrant colonies in bolivia and bolivian natives. | this study was performed to estimate human t-cell leukemia-lymphoma virus type-i (htlv-i) infection and human immunodeficiency virus (hiv) infection in japanese immigrant colonies in bolivia, where no seroepidemiological study of htlv-i or hiv has ever been reported, among 647 healthy adults and children of japanese descent and bolivian natives living in the same colonies. the overall prevalence of htlv-i antibody was 12.6% (59/469) among japanese immigrant populations, but increased with age, b ... | 1987 | 2892821 |
| stimulation of muscarinic receptor in hippocampal neuron induces characteristic increase in cytosolic free ca2+ concentration. | changes in cytosolic free ca2+ concentration ([ca2+]i) in response to acetylcholine (ach) were examined by fura-2 fluorometry in cultured rat hippocampal neurons. ach (greater than or equal to 10(-5) m) induced an increase in [ca2+]i composed of fast transient and slow long-lasting phases. atropine (10(-8) m) abolished the fast component and greatly reduced the slow component. the slow component was selectively blocked by pirenzepine (10(-6) m). the effect of ach remained partially in a ca2+-def ... | 1988 | 2896321 |
| regional levels of neurotransmitter markers in the pigeon telencephalon: a comparison with possibly homologous areas of the rat telencephalon. | the levels of cholinergic, gamma-aminobutyric acidergic (gabaergic), and excitatory amino acid neurotransmitter markers have been measured in 18 regions of the pigeon telencephalon as well as in supposedly homologous areas of the rat telencephalon. among the basal telencephalic areas, some similar patterns of regional distribution were observed, with the noticeable exception of the ratio of levels of cholinergic markers between the striatum and globus pallidus, which was much larger in the rat t ... | 1988 | 2897425 |
| effect of sodium valproate on midazolam distribution. | the displacement of midazolam, a new water-soluble, short acting benzodiazepine, from its plasma binding sites by sodium valproate, has been studied in man. an increase of its free fraction (ranging from 2.71 to 5.35%) in plasma from epileptic patients receiving sodium valproate was observed. a similar situation was created in rabbits by pretreatment with sodium valproate (600 mg kg-1 day-1) and posterior hypnosis with midazolam. due to the interaction, sodium valproate-pretreated rabbits showed ... | 1988 | 2897455 |
| repeated treatment with imipramine and amitriptyline reduced the immobility of rats in the swimming test by enhancing dopamine mechanisms in the nucleus accumbens. | bilateral injections of 1 microgram sulpiride in the rat nucleus accumbens antagonized the effect of a seven-day treatment with 20 mg kg-1 day-1 imipramine or amitriptyline in the swimming test. the data suggest that dopamine mechanisms in the limbic regions of the rat brain are involved in the effect of repeated treatment with imipramine and amitriptyline in that test. | 1988 | 2897457 |
| diagnosis of endocarditis in acute q-fever by immunofluorescence serology. | the authors compared two groups of 20 patients suffering from q fever using microimmunofluorescence (micro if) serology. one group had endocarditis and the other conventional symptoms of acute q fever but no endocarditis. determination of the levels of antibodies against the two phases of rickettsiae in each of the three immunoglobulin classes (igg, igm and iga), allowed to determine the type of infection using a single serum sample. patients having iga class antiphase i antibodies at a level eq ... | 1988 | 2897775 |
| striatal grafts in rats with unilateral neostriatal lesions--i. ultrastructural evidence of afferent synaptic inputs from the host nigrostriatal pathway. | tyrosine hydroxylase immunocytochemistry, in combination with golgi impregnation, has been used to study the dopaminergic afferent input to striatal suspension grafts implanted into the previously ibotenic acid-lesioned striatum in adult recipient rats. the rats were perfused for combined light- and electron microscopy at 10-11 months after transplantation, at the end of a series of behavioural experiments and a study of in vivo gaba release, reported in the two accompanying papers. a tyrosine h ... | 1988 | 2898109 |
| isospora belli enteric infection in patients with human t-cell leukemia virus type i-associated adult t-cell leukemia. | | 1988 | 2901228 |
| a major antigen from mycobacterium tuberculosis which is homologous to the heat shock proteins groes from e. coli and the htpa gene product of coxiella burneti. | | 1988 | 2902558 |
| immunity in q fever. | post-infection and post-vaccination immune mechanisms in q fever are summarized. whereas cell-mediated immunity has been found to play a crucial role in developing resistance to coxiella burnetii infection, data on the role of specific antibodies in q fever immunity are controversial. the functional state of immunocompetent cells and professional phagocytes seems to be decisive for the persistence of c. burnetii within phagocytic cells and for the control of q fever at the host level. defects of ... | 1988 | 2903642 |
| kinetics of phospholipid extraction from coxiella burnetii. | kinetics of phospholipid extraction from purified suspensions of coxiella burnetii in phase i by various chloroform--methanol mixtures at various temperatures were evaluated based on the amount of phosphorus extracted. extraction by a boiling (53 degrees c) 2:1 chloroform:methanol mixture proved to be the most efficient. | 1988 | 2904741 |
| ornithine decarboxylase activity and the accumulation of its mrna during early stages of liver regeneration. | the marked and well documented stimulation of hepatic ornithine decarboxylase (odc; ec 4.1.1.17) in response to partial hepatectomy is at least to some extent attributable to an enhanced accumulation of the enzyme's mrna. the stimulation of odc activity was associated with an increased accumulation of two odc-related mrna species (2.1 and 2.6 kilobases; kb) as revealed by northern blot hybridization analyses. the levels of the above-mentioned messages remained elevated for 6 h after partial hepa ... | 1989 | 2909238 |
| investigation of the active center of rat pancreatic elastase. | we have isolated rat pancreatic elastase i (ec 3.4.21.36) using a fast two-step procedure and we have investigated its active center with p-nitroanilide substrates and trifluoroacetylated inhibitors. these ligands were also used to probe porcine pancreatic elastase i whose amino acid sequence is 84% homologous to rat pancreatic elastase i as reported by macdonald, et al. (biochemistry 21, (1982) 1453-1463). both proteinases exhibited non-michaelian kinetics for substrates composed of three or fo ... | 1989 | 2909256 |
| amiloride effect on diurnal cyclic na and k excretion in rats. | amiloride was administered to rats during the peak and minimum of na and k diurnal rhythmic excretion (i.e., during early dark phase and early light phase). in rats receiving a normal-k diet (2.34 meq/day) amiloride decreased k excretion from 186 to 37 mueq/h (dark phase) and from 31 to 4 mueq/h (light phase). amiloride increased na excretion from 91 to 344 mueq/h (dark phase) and from 35 to 164 mueq/h (light phase). rats receiving a high-k diet (10.4 meq/day) showed a higher diurnal peak and mi ... | 1989 | 2916703 |
| truckin' pneumonia--an outbreak of q fever in a truck repair plant probably due to aerosols from clothing contaminated by contact with newborn kittens. | we describe an outbreak of q fever affecting 16 of 32 employees at a truck repair plant. none of the cases were exposed to cattle, sheep or goats, the traditional reservoirs of q fever. the cases did not work, live on, or visit farms or attend livestock auctions. one of the employees had a cat which gave birth to kittens 2 weeks prior to the first case of q fever in the plant. the cat owner fed the kittens every day before coming to work as the cat would not let the kittens suckle. serum from th ... | 1989 | 2917613 |
| effects of human fetal liver extract on the growth of hl-60 cells. | two kinds of hl-60 cell growth suppressor present in human fetal liver were studied. one is the already known arginase, which showed nonspecific suppression on the growth of hl-60 cells and human granulocyte-macrophage progenitors (cfu-gm), and the other, a new species of suppressor with the characteristics of lower molecular weight (less than 10,000 daltons), shows preferential suppression on hl-60 cell growth, but less effect on the growth of cfu-gm. | 1989 | 2917625 |
| glucocorticoid receptor activation in isolated perfused rat hearts. | the formation of unactivated and activated glucocorticoid receptor complexes was studied in intact, isolated, perfused rat hearts in the presence of [3h]triamcinolone acetonide. receptor activation, as quantified by the dna-cellulose-binding assay, began to increase within 30 s of perfusion and reached a final steady-state level (t 1/2 = 4.6 min) with 46% of the steroid-receptor complexes bound to dna-cellulose. with the use of a linear potassium phosphate (kp) gradient (5-400 mm), unactivated r ... | 1989 | 2919654 |
| differences in cyclophosphamide-induced suppression of cricket predation in selectively bred strains of taste-aversion prone and resistant rats. | cyclophosphamide-induced conditioned suppression of cricket predation was observed in taste-aversion-prone (tap) but not in taste-aversion-resistant (tar) rats. these tap and tar strains had been selectively bred for efficient or inefficient acquisition of cyclophosphamide-induced saccharin taste aversions (tas). equivalent preconditioning cricket predation was practiced by nonfasted subjects of both strains. tar rats that ate crickets before a cyclophosphamide injection were thereafter voraciou ... | 1989 | 2923664 |
| differential ontogeny of working memory and reference memory in the rat. | in two experiments we examined the ability of 15-, 21-, and 27-day-old rats to perform two spatial working memory problems (delayed alternation and discrete-trials delayed alternation) and a reference memory problem (position habit) in a t-maze. in the delayed alternation problem, each animal was presented with a series of free-choice trials and was rewarded for regularly alternating responses to the left and right arms of the t-maze. in the discrete-trials delayed alternation problem, each anim ... | 1989 | 2923681 |
| coxiella burnetii infection (q fever). | | 1989 | 2925495 |
| carbohydrate content of the endolymphatic sac. a histochemical and lectin-labelling study in the mongolian gerbil. | a secretory activity has recently been attributed to the endolymphatic sac (es), as a possible way to contribute to the fluid balance of the entire endolymphatic compartment. previous histochemical studies have indicated the existence of carbohydrate complexes in the secretory product, both neutral and acidic in nature. a thorough analysis of these compounds in the gerbilline es was carried out using both transmission electron microscopic (tem) histochemistry, using dialyzed iron and periodic ac ... | 1989 | 2926257 |
| age-related changes in regional patterns of blood-brain barrier breakdown during epileptiform seizures induced by pentylenetetrazol. | age related changes in blood-brain barrier (bbb) permeability to macromolecules were investigated during seizures induced by pentylenetetrazol in rats aged from 6 to 120 days. evans blue was used as a visual indicator of bbb integrity. bbb leakage due to seizures was present only in animals in which the mean arterial blood pressure (bp) rose with the seizure onset. although considerable bbb damage was found partly in similar areas in young and adult rat brains, in adults the leakage of evans blu ... | 1989 | 2927722 |
| [unusual manifestations of q fever disclosing hairy cell leukemia]. | an unusual case of q fever in a 62-year-old female is described. the patient presented with severe pneumonia and developed renal failure, disseminated intravascular coagulation and pancytopenia which recurred after antibiotics were discontinued. subsequently hairy cell leukemia was diagnosed and evolved favorably under treatment with doxycycline and alpha-interferon. a review of the literature confirms that renal failure and disseminated intravascular coagulation do not appear to be associated w ... | 1989 | 2928742 |
| chronic q fever with mixed cryoglobulinaemia. | a 47 year old woman presented with a six month history of vasculitic rash, splenomegaly, and cardiac murmurs. investigations showed the presence of mixed cryoglobulinaemia and raised titres to coxiella burnetii consistent with chronic q fever infection. the patient was treated with tetracycline (1 g four times a day). | 1989 | 2930282 |
| primary structure of single-chain pro-urokinase. | single-chain pro-urokinase is an inactive proenzyme form of human urokinase with a single-chain structure and a mr of 50,000 and converted to the active two-chain form by catalytic amounts of plasmin. it was isolated from culture fluid of human kidney cells and subjected to chemical (cnbr) and proteolytic (lysyl endopeptidase) degradation. the resulting peptides were separated by reverse-phase high performance liquid chromatography and subjected to automated sequence analysis. amino acid sequenc ... | 1985 | 2931434 |
| [present and future value of monoclonal antibodies in nephrology]. | many monoclonal antibodies directed against human t-lymphocytes have been produced in mice. some of these, such as okt 3, recognize all t-cells which can thus be counted in the blood; they have been used for the prevention of renal transplant rejection. despite unexpected problems, such as antigenic modulation and anti-mouse sensitization, the first results obtained are promising. other antibodies produced are directed against t-lymphocyte subsets, notably t-helpers (okt4+) and t-suppressors or ... | 1985 | 2933669 |
| demonstration in hungary of q fever associated with abortions in cattle and sheep. | | 1985 | 2940838 |
| dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility. | in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ... | 1986 | 2942039 |
| human t helper cells specific for antigens of typhus group rickettsiae enhance natural killer cell activity in vitro. | the peripheral blood mononuclear cells (pbmc) from 5 individuals immune to typhus group rickettsiae and from 13 nonimmune individuals were stimulated in vitro for 7 days with typhus group rickettsial antigen (tgra). at the end of day 7, lysis of the natural killer (nk)-susceptible target k562 by these pbmc was determined. as controls, pbmc from both groups of donors were cultured in vitro for 7 days without antigen or were freshly isolated, and lysis of the k562 target was determined. there was ... | 1986 | 2945787 |
| [experimental q fever glomerulonephritis in guinea pigs]. | | 1986 | 2946441 |
| [effects of my-5116 on experimental animal models of type i-type iv allergic reactions and the formation of ige antibody]. | the effects of a new anti-allergic agent, my-5116: isoamyl 5,6-dihydro-7,8-dimethyl-4,5-dioxo-4h-pyrano [3,2-c] quinoline-2-carboxylate, on experimental animal models of type i approximately type iv allergic reactions and the formation of ige antibody were investigated. my-5116 administered intraperitoneally at doses of 30 and 100 mg/kg suppressed significantly the homologous pca in guinea pigs. my-5116 administered intraperitoneally at the dose of 100 mg/kg also suppressed significantly the het ... | 1986 | 2947841 |
| isolation and analysis of escherichia coli mutants that allow increased replication of bacteriophage lambda. | escherichia coli mutants were isolated that supported the growth of a lambda ots and, in at least one case, a lambda bts phage at the normally nonpermissive temperature of 39 degrees c. in one such strain, ots and bts suppression ability appeared to be a function of the guab gene. ots suppression by the mutant guab strain was prevented if high levels of guanine or xanthine were present in the medium. no other base had any effect on ots suppression in this strain. other strains carrying spontaneo ... | 1987 | 2951367 |
| [interferon production in vitro]. | interferon production in vitro was induced by poly i: c and pha in leukocytes obtained from patients with cancer, benign diseases and control subjects. the interferon response per lymphocyte was relatively constant in all groups. however, interferon production was slightly inhibited in patients receiving cancer chemotherapy. the peak response of interferon occurred at 48 hours after the initiation of the combined culture in each specimen. from our observations, it was suggested that interferon p ... | 1987 | 2952067 |
| yeast-recombinant hepatitis b vaccine. efficacy with hepatitis b immune globulin in prevention of perinatal hepatitis b virus transmission. | a yeast-recombinant hepatitis b vaccine was licensed recently by the food and drug administration and is now available. to assess the efficacy of the yeast-recombinant vaccine, we administered the vaccine in combination with hepatitis b immune globulin to high-risk newborns. if infants whose mothers were positive for both hepatitis b surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. among infants in ... | 1987 | 2952812 |
| yeast-recombinant hepatitis b vaccine. efficacy with hepatitis b immune globulin in prevention of perinatal hepatitis b virus transmission. | a yeast-recombinant hepatitis b vaccine was licensed recently by the food and drug administration and is now available. to assess the efficacy of the yeast-recombinant vaccine, we administered the vaccine in combination with hepatitis b immune globulin to high-risk newborns. if infants whose mothers were positive for both hepatitis b surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. among infants in ... | 1987 | 2952812 |
| [listeria rhomboencephalitis without meningeal involvement]. | | 1987 | 2953019 |
| [q fever infectious endocarditis. 3 cases]. | an acute infectious disease with predominant pulmonary symptoms, q fever, may become chronic as hepatitis or, more frequently, endocarditis. we report 3 cases of q fever endocarditis. in 2 of these patients endocarditis developed on cardiac valve prosthesis. the 3 patients have been under doxycycline for more than a year, and their condition is satisfactory. a review of the literature provides additional data on the epidemiological, aetiological, clinical, biological and therapeutic aspects of t ... | 1987 | 2954120 |
| multiple low-dose streptozotocin-induced diabetes in the mouse: further evidence for involvement of an anti-b cell cytotoxic cellular auto-immune response. | anti-b cell auto-immunity may play a role in the pathogenesis of diabetes in mice resulting from multiple subdiabetogenic doses of the pancreatic b cell toxin, streptozotocin. in the present study we have investigated the cytotoxic anti-b cell response in these mice. a major role for b lymphocytes, macrophages, or their products in the cytotoxic response originally detected in vitro was eliminated by passing splenocytes from the mice treated with multiple subdiabetogenic doses of streptozotocin ... | 1987 | 2954873 |
| [a case of q fever in moravia]. | | 1987 | 2959380 |
| studies on some aspects of glucose metabolism in guinea pigs infected with coxiella burnetii. | | 1987 | 2961686 |
| transcriptional mapping and nucleotide sequence of the escherichia coli fepa-fes enterobactin region. identification of a unique iron-regulated bidirectional promoter. | the iron-controlled fepa and fes-entf transcripts from the escherichia coli enterobactin gene complex are expressed divergently from a limited genetic region, thereby suggesting the existence of a single, possibly overlapping promoter junction for these genes. the nucleotide sequence of a 1,997-base pair hpai fragment specific for this genetic region allowed for the identification of an 1,122-base pair open reading frame as the previously uncharacterized fes gene. its product, fes (approximately ... | 1988 | 2974033 |
| pulmonary macrophage function during experimental cytomegalovirus interstitial pneumonia. | since cytomegalovirus (cmv) infections may alter host defense against a variety of pathogens, phagocytosis, oxygen uptake, and h2o2 release by pulmonary macrophages obtained from guinea pigs with acute cmv interstitial pneumonia were evaluated. experimental animals were inoculated subcutaneously on day zero with 10(7.5) 50% tissue culture infective doses of virulent guinea pig cmv. control animals received an uninfected salivary gland suspension. the animals were sacrificed on day 7; the tissues ... | 1985 | 2981196 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| [vaccination of animals and human health]. | prophylactic immunization of animals against obligat and nonobligat pathogenic zoonoses benefit human health in many ways both directly and indirectly. typical examples of a direct protective effect are the vaccinations of dogs, cats and foxes against rabies as well as the vaccinations against respiratory diseases in cows, horses, dogs and cats to which the most varied species of pathogens of noncompulsory zoonoses contribute. a considerable contribution to the protection of human health is made ... | 1985 | 2986381 |
| human monoclonal antibodies against mycobacterium leprae. | human hybridomas were constructed which produce antibodies against three different extracts of mycobacterium leprae. a thioguanine-resistant (thgr), ouabain-resistant (ouar), human lymphoblastoid cell line, kr-4, was hybridized with epstein-barr virus-transformed cell lines from lepromatous leprosy patients with fusion frequencies of greater than 10(-5). non-epstein-barr virus-transformed donor cells fused at much lower rates (less than 2 x 10(-7]. hybrids were selected in medium containing hypo ... | 1985 | 2989177 |
| insulin-sensitive phosphodiesterase and insulin receptor binding in fat cells from spontaneously obese rats. | the effects of insulin on insulin-sensitive phosphodiesterase were investigated in fat cells from rats aged 4, 8 and 16 weeks. the enzyme activities in rats aged 4 and 8 weeks higher at 0.1-30 nmol/l insulin concentrations than in rats aged 16 weeks, and half-maximum stimulations were obtained at 0.08 nmol/l in rats aged 4 weeks, at 0.15 nmol/l in rats aged 8 weeks and at 0.22 nmol/l in rats aged 16 weeks. specific binding of insulin in fat cells from rats aged 4, 8 and 16 weeks was 3.3%, 5.0% a ... | 1985 | 2991053 |
| sphingomyelin synthesis in hymenolepis diminuta (cestoda). | adult hymenolepis diminuta incorporated label from l[u-14c]serine, [1-14c]palmitic acid, [1-14c]palmitoyl-coa and cytidine-5'-diphospho[methyl-14c]choline into the various intermediates of sphingomyelin synthesis (ketosphingosine, dihydrosphingosine, sphingosine, ceramide and sphingomyelin). from the results it was concluded that h. diminuta possessed the five enzymes involved in sphingomyelin synthesis, namely serine palmitoyl-transferase, 3-oxosphinganine reductase, flavoprotein dihydrosphingo ... | 1985 | 2993879 |
| primary cultures from defined brain areas; effects of seeding time on the development of beta-adrenergic- and dopamine-stimulated camp-activity during cultivation. | the influence of seeding time on the expression of beta-adrenergic- and dopamine-stimulated camp activity was studied in primary astroglial cultures from various brain areas. in all cultures isoproterenol (10(-7)-10(-5) m) caused an increased camp accumulation with time in culture. in brainstem cultures from 17-day-old embryos there was a decreased intracellular camp accumulation between 3 and 4 weeks. in all newborn rats and in striatal cultures from 7-day-postnatal rats there was a similar dec ... | 1985 | 2994849 |
| genomic analysis of phase i and ii coxiella burnetii with restriction endonucleases. | restriction endonuclease-digested dnas from several isolates of phase i and phase ii coxiella burnetii were compared using agarose gel electrophoresis and soft-laser scanning densitometry. our results demonstrate that the two phases are, as previously assumed, alternative phases of the same organism. although the restriction endonuclease digestion revealed genetic differences between clonal isolates of phase i and phase ii c. burnetii nine mile strain, these differences do not appear to be relat ... | 1985 | 2995547 |
| activity of the cytomegalovirus genome in the presence of ppi analogs. | ppi analogs and esters of these were studied for their effect on cytomegalovirus (cmv) multiplication. five aromatic monoesters of phosphonoformate esterified either in the phosphono or the carboxylic group and two diesters were demonstrated to inhibit cmv dna synthesis and late viral protein synthesis. in a direct assay, the monoesters but not the diesters inhibited cmv dna polymerase activity. the production of early cmv antigens was not inhibited by any of the compounds. after incubation with ... | 1985 | 2999452 |
| in vitro binding of platinum compounds to human transferrin. | the binding of the antineoplastic drug cis-dichlorodiammineplatinum ii (cddp) and a related complex, potassium tetrachloroplatinite (k2ptcl4), to human transferrin in buffered anion solutions was studied over a period of two weeks. separation of bound and free platinum was accomplished by gel chromatography with platinum analysis by atomic absorption spectrophotometry. the results demonstrated that k2ptcl4 was bound to a greater degree than cddp in this system with 3-5 and 1-2 platinum atoms res ... | 1985 | 3001886 |
| nucleotide sequence and in vivo expression of the ilvy and ilvc genes in escherichia coli k12. transcription from divergent overlapping promoters. | the ilvc gene of escherichia coli k12 encodes acetohydroxy acid isomeroreductase, the second enzyme in the parallel isoleucine-valine biosynthetic pathway. previous data have shown that transcription of the ilvc gene is induced by the acetohydroxy acid isomeroreductase substrates, acetohydroxybutyrate or acetolactate, and that this substrate induction of ilvc expression is mediated by a positive activator encoded by the ilvy gene. we report here the isolation and complete nucleotide sequence of ... | 1986 | 3003115 |
| alpha 2 adrenoceptors modulate the nociceptive jaw-opening reflex in rats. | the jaw-opening reflex in lightly anesthetized rats induced by intrapulpal (left maxillary) electrical tooth pulp stimulation and quantified by the electromyograms (threshold values) recorded from the ipsilateral digastric muscle was used as the experimental pain index. the threshold for the jaw-opening reflex was significantly elevated by clonidine (12.5 to 50 micrograms/kg, i.v.) and was inversely correlated with the frequency of stimulation. the analgesia elicited by clonidine was antagonized ... | 1986 | 3005019 |
| alpha 2 adrenoceptors modulate the nociceptive jaw-opening reflex in rats. | the jaw-opening reflex in lightly anesthetized rats induced by intrapulpal (left maxillary) electrical tooth pulp stimulation and quantified by the electromyograms (threshold values) recorded from the ipsilateral digastric muscle was used as the experimental pain index. the threshold for the jaw-opening reflex was significantly elevated by clonidine (12.5 to 50 micrograms/kg, i.v.) and was inversely correlated with the frequency of stimulation. the analgesia elicited by clonidine was antagonized ... | 1986 | 3005019 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. | the 3' end of the 20-kb genome of the purdue strain of porcine transmissible gastroenteritis coronavirus (tgev) was copied into cdna after priming with oligo(dt) and the double-stranded product was cloned into the psti site of the puc9 vector. one clone of 2.0-kb contained part of the poly(a) tail and was sequenced in its entirety using the chemical method of maxam and gilbert. another clone of 0.7 kb also contained part of the poly(a) tail and was sequenced in part to confirm the primary struct ... | 1986 | 3008432 |
| multinucleation versus "cell-in-a-cell" pattern. | | 1986 | 3008473 |
| genetic heterogeneity among isolates of coxiella burnetii. | chromosomal and plasmid dna have been extracted from six isolates of coxiella burnetii, the aetiological agent of q fever. restriction fragment length polymorphisms detected after haeiii digestions of chromosomal dna revealed four different patterns that distinguished the american from the european isolates, and the nine mile phase i prototype strain from a spontaneously derived, isogenic phase ii nonrevertant variant. at least one of the haeiii fragments visible in the pattern from nine mile ph ... | 1986 | 3011963 |
| activation of h-ras-1 oncogenes by chemical carcinogens. | | 1986 | 3017289 |
| cellular oncogenes and the pathogenesis of human cancer. | | 1986 | 3019863 |
| cellular oncogenes and the pathogenesis of human cancer. | | 1986 | 3019863 |
| stlv-i antibodies in feral populations of east african vervet monkeys (cercopithecus aethiops). | serum samples from feral populations of african green monkeys (cercopithecus aethiops) were screened for antibodies to the simian t-lymphotropic virus, type i (stlv-i). blood samples had been collected from 336 monkeys in 4 regions of central and southern kenya in 1978 and 1979, from 114 monkeys in central ethiopia in 1973, and from 85 monkeys from the kampala region of uganda in 1966. a total of 178/535 monkeys (33%) were seropositive (stlv-i+). only 4/114 monkeys (4%) from ethiopia were seropo ... | 1986 | 3019900 |
| stlv-i antibodies in feral populations of east african vervet monkeys (cercopithecus aethiops). | serum samples from feral populations of african green monkeys (cercopithecus aethiops) were screened for antibodies to the simian t-lymphotropic virus, type i (stlv-i). blood samples had been collected from 336 monkeys in 4 regions of central and southern kenya in 1978 and 1979, from 114 monkeys in central ethiopia in 1973, and from 85 monkeys from the kampala region of uganda in 1966. a total of 178/535 monkeys (33%) were seropositive (stlv-i+). only 4/114 monkeys (4%) from ethiopia were seropo ... | 1986 | 3019900 |
| structure-activity relationships of opioid peptides. | | 1986 | 3020417 |
| structure-activity relationships of opioid peptides. | | 1986 | 3020417 |
| [pathogenesis and etiologic treatment of herpetic stomatitis]. | | 1985 | 3020482 |
| [pathogenesis and etiologic treatment of herpetic stomatitis]. | | 1985 | 3020482 |
| salicylate effects on proton gradient dissipation by isolated gastric mucosal surface cells. | the effects of salicylate were examined on na+/h+ exchange by isolated gastric mucosal surface cells loaded with h+ and resuspended in a buffered medium. choline salicylate (ph 7.4) increases the dissipation of an intracellular proton gradient which was measured using acridine orange. the exchange of extracellular na+ with intracellular h+ by surface cells not only remains intact but also is enhanced upon exposure to salicylate. this was confirmed by cellular uptake of 22na and titration of cell ... | 1986 | 3020564 |
| salicylate effects on proton gradient dissipation by isolated gastric mucosal surface cells. | the effects of salicylate were examined on na+/h+ exchange by isolated gastric mucosal surface cells loaded with h+ and resuspended in a buffered medium. choline salicylate (ph 7.4) increases the dissipation of an intracellular proton gradient which was measured using acridine orange. the exchange of extracellular na+ with intracellular h+ by surface cells not only remains intact but also is enhanced upon exposure to salicylate. this was confirmed by cellular uptake of 22na and titration of cell ... | 1986 | 3020564 |
| [cattle calcinosis in the lower austria alpine foothills area]. | it is reported on cases of calcinosis during winter 1979/80 occurring on 42 farms and leading to 34 forced slaughters and on the control of this disease in a localized region of the alpine foot-hills. instruction of the farmers, ploughing up of the meadows with following new sowing of grass without golden oat (trisetum flavescens) allowed to push back calcinosis in high situated especially endangered farms. the different calcinogenic activity of golden oat in the different stages of growing, pus ... | 1986 | 3020735 |
| [cattle calcinosis in the lower austria alpine foothills area]. | it is reported on cases of calcinosis during winter 1979/80 occurring on 42 farms and leading to 34 forced slaughters and on the control of this disease in a localized region of the alpine foot-hills. instruction of the farmers, ploughing up of the meadows with following new sowing of grass without golden oat (trisetum flavescens) allowed to push back calcinosis in high situated especially endangered farms. the different calcinogenic activity of golden oat in the different stages of growing, pus ... | 1986 | 3020735 |
| a distinct glucocorticoid hormone response regulates phosphoprotein maturation in rat hepatoma cells. | glucocorticoid hormone-dependent maturation of the mouse mammary tumor virus (mmtv) phosphorylated polyprotein (pr74) allows experimental access to certain posttranslational regulatory circuits under steroid control in m1.54 cells, an mmtv-infected rat hepatoma cell line. pulse-chase experiments revealed that [35s]methionine-labeled pr74 synthesized in uninduced cells could be converted posttranslationally into p24, a stable phosphorylated maturation product, only after 4 h of exposure to 1 micr ... | 1986 | 3023857 |
| a distinct glucocorticoid hormone response regulates phosphoprotein maturation in rat hepatoma cells. | glucocorticoid hormone-dependent maturation of the mouse mammary tumor virus (mmtv) phosphorylated polyprotein (pr74) allows experimental access to certain posttranslational regulatory circuits under steroid control in m1.54 cells, an mmtv-infected rat hepatoma cell line. pulse-chase experiments revealed that [35s]methionine-labeled pr74 synthesized in uninduced cells could be converted posttranslationally into p24, a stable phosphorylated maturation product, only after 4 h of exposure to 1 micr ... | 1986 | 3023857 |