| effect of human immunoglobulin preparations on fc rosette formation between anti-d-coated erythrocytes and lymphocytes. | human immunoglobulin (ig) preparations were tested for their inhibitory effect on fc rosette formation between anti-d-coated human erythrocytes and lymphocytes, as compared to their complement activating capacity. both of the two biological activities ascribed to the sites in the fc portion of the igg molecules were found to be reduced in the pepsin-treated, as well as in the s-sulfonated ig preparations, as compared to the activities of the normal human ig preparation. in the plasmin-treated ig ... | 1978 | 150144 |
| 2'-chloro-1-hydroxy-2-naphthanilide-4'-isothiocyanate--a new cestodicidal agent [1]. | 2'-chloro-1-hydroxy-2-naphthanilide-4'-isothiocyanate (4) has been synthesized as the structural analogue of yomesan (1) and was found to be active against experimental dwarf tapeworm hymenolepis nana infection in rats at an oral dose of 7.5 mg/kg. | 1978 | 150149 |
| [raised prostaglandin e-2 level in focal inflammatory lesions of the human skin with particular reference to rosacea]. | | 1978 | 151301 |
| [studies of the permeability of human dentin part i: basic studies of constant physiological parameters and experimental variables (author's transl)]. | diffusion processes proceeding in the dentin are of fundamental scientific interest and of major importance to numerous therapeutical measures, with the permeability of dentin playing an essential role. their exact quantitative determination is possibly in vitro only and depends upon various factors. relevant investigative results obtained using standardized parameters and the methods employed are subjected to a critical analysis and interpreted with regard to the possibility of using them for s ... | 1978 | 151421 |
| an immunochemical difference between myosins from normal and hypertrophied rat hearts. | | 1978 | 151633 |
| immune response-associated antigens on mouse leukemia cells. ii. anti-ia sera inhibit the mlr reaction between normal gr spleen cells and syngeneic spleen cells of grsl tumor-bearing mice. | mitomycin c-treated ascites cells of ia antigen-positive grsl14 tumor cells and spleen cells from grsl14 tumor-bearing mice stimulated lymphocyte proliferative responses in normal syngeneic gr spleen cells. furthermore, mitomycin c-treated t cells purified from spleen cells of tumor-bearing mice also stimulated normal gr spleen cells. anti-ia sera inhibited the stimulating ability of tumor cells and of spleen cells of tumor-bearing mice. these data suggest a role for i region gene products in im ... | 1978 | 151953 |
| a simple microassay for inorganic phosphate, ii. | | 1978 | 152065 |
| some behavioral effects of serotonin depletion depend on method: a comparison of 5,7-dihydroxytryptamine, p-chlorophenylalanine, p-choloroamphetamine, and electrolytic raphe lesions. | | 1978 | 152081 |
| isolation and localization from chicken gizzard of an inhibitory protein for mg2+-activated skeletal muscle actomyosin atpase. | an inhibitory protein for mg2+-activated actomyosin atpase from rabbit skeletal muscle was prepared from frozen chicken gizzard and purified by deae-sephadex chromatography and gel filtration. 2. the inhibition by this protein was released by the addition of skeletal muscle troponin c and was independent of gizzard tropomyosin. 3. localization of the inhibitory protein in gizzard muscle tissue and gizzard thin filaments was demonstrated by immunohistological techniques and immunodiffusion tests. | 1978 | 152312 |
| the effect of hypervitaminosis a on rat palatal development. | retinoic acid or retinyl acetate was administered to pregnant rats in doses sufficient to induce a 90% incidence of cleft palate. in another study, a delay in the reorientation of the palatal shelves was observed to be longer with the more potent teratogen, retinoic acid. on day 16 of gestation, 24 hours after final dosage with vitamin a, the synthesis of dna and protein was studied in fetal carcass, mandible, and palate, and that of sulfated mucopolysaccharides (s-mps) and glycoproteins (gp) in ... | 1978 | 152485 |
| [biochemistry of myocardium taken at autopsy. preliminary report]. | the findings after biochemical analysis of heart muscle taken at autopsy are given in this preliminary communication. human myosin is made up of two heavy sub-units and two light sub-units: it is similar to cardiac myosin found in other mammals, but is different in certain characteristics, particularly immunological ones. tropomyosin is made up of two different sub-units. the normal human heart contains 1 mg of collagen and 130 microgram of desoxyribonucleic acid (dna) per 100 mg of fresh tissue ... | 1978 | 152617 |
| trypanosoma cruzi: isolation and characterization of membrane and flagellar fractions. | | 1978 | 153234 |
| [the value of various radiological procedures for the investigation of functional disturbances of renal transplants (author's transl)]. | the value of various radiological examinations was analysed with reference to 75 renal transplants which had developed complications. if the renal poles are marked with metallic clips, the size of the transplant can be estimated by abdominal x-rays. abnormal size was found exclusively in severe rejection reactions, but only about 50% of acute rejections showed such a change. contrast examinations were found to be valuable in the diagnosis or exclusion of anatomically localised lesions such as ob ... | 1978 | 153266 |
| studies of the chemo-mechanical conversion in artificially produced streamings. ii. an order--disorder phase transition in the chemo-mechanical conversion. | | 1978 | 153344 |
| lymphocyte chalone. | | 1978 | 153402 |
| biosynthesis of estrogens in pregnant and non-pregnant guinea pigs. | guinea pig ovaries obtained from pregnant and non-pregnant animals, incubated in the presence of a nadph-generating system, converted [3h]-dehydroepiandrosterone and [14c]testosterone to estradiol. under the same conditions, no radioactive estrogen could be identified in adrenal and placental incubations. the rate of formation of estradiol from dehydroepiandrosterone was linear during the 30-min incubation. the initial rates of formation for estradiol were 415 x 10(-13) and 10.4 x 10(-13) mol/mi ... | 1978 | 154398 |
| isolation and characterization of fragments of reduced and s-carbamidomethylated human growth hormone produced by plasmin digestion. i. chemistry. | reduced and s-carbamidomethylated human gh (rcam-hgh) was digested with human plasmin, yielding a mixture of products. these were partially separated by chromatography on deae-cellulose, yielding three major fractions: da and db, which were equipotent with native hgh in the weight gain test; and dc, which was about half as active as hgh. each of these was further purified by gel filtration, yielding a number of subfractions which were characterized as follows: da1 is a very stable noncovalent co ... | 1978 | 154400 |
| changes in somatostatin concentration in pancreas and other tissues of streptozotocin diabetic rats. | changes in immunoreactive somatostatin were examined in islets, whole pancreas, stomach, and hypothalamus of streptozotocin-diabetic rats. there was no change in islet somatostatin content at 2 days after the administration of streptozotocin, but thereafter, somatostatin progressively increased in the diabetic animals by 45% at 2 weeks, 230% at 6 weeks, and 500% by 6 months. by contrast, islet glucagon rose acutely and maintained a constant 2-fold elevation irrespective of the duration of the di ... | 1978 | 154402 |
| [occupational dermatoses in workers of a building combine]. | examinations involved 33 building combine workers. findings were as follows: occupational acne in 94% of workers, toxic dermatitis of epidermis and skin in 42% and allergic dermatitis of epidermis and skin in 9% of workers, resulting from work in exposure to cement, lime and oils. | 1978 | 154604 |
| enhancement by caffeine of neocarzinostatin cytotoxicity in murine leukemia l1210 cells. | posttreatment incubation with nontoxic doses of caffeine resulted in enhancement of cell lethality and inhibition of cell growth in l1210 mouse leukemia cells which had been exposed to a protein antibiotic, neocarzinostatin. in addition, caffeine treatment appeared to inhibit the eventual maturation of newly synthesized dna in l1210 cells following exposure to this antibiotic. these results, indicating the existence of caffeine-sensitive repair in l1210 leukemia cells treated with neocarzinostat ... | 1979 | 154973 |
| arterio-venous shunt with percutaneous leads in calves for long-term in vivo testing of blood-contact materials. | in six calves an extracorporeal arteriovenous shunt in combination with a successful type of percutaneous lead has been implanted. flow chambers with various test materials can be inserted. preliminary results show that this shunt can be used for long term tests for evaluation of blood contact material and studies about thrombus formation in an ex vivo setting as well as for permanent blood access for the purpose of various measurements, drug application and for hemodialysis. | 1979 | 155070 |
| muscle spindle discharge in normal and obstructed movements. | 1. the discharge activity of muscle spindle endings located in tail and hind limb muscles was recorded during voluntary movements in the cat. 2. during active shortening of the receptor-bearing muscles, both primary and secondary endings tended to fall silent. this was more pronounced, the higher the rate of muscle shortening. we suggest that in unobstructed movements in which muscle velocities exceed 0.2 resting lengths per second (lr/sec), the firing patterns of spindle afferents are dominated ... | 1979 | 155160 |
| chemistry and pharmacokinetics of ipratropium bromide. | | 1979 | 155287 |
| early changes in the arterial endothelium under various pathological conditions: an electron histochemical study with visualization of altered permeability using electron microscopic tracers. | the authors have studied the endothelial changes in the aorta and the arteries of heart, kidney and other organs in cholesterol fed rabbits and endotoxin administrated rats through electron histochemical and vascular clearance methods. the vascular changes are variable presumably due to the functional and morphological difference of vessels. the administration of cholesterol and endotoxin may result in increased vascular permeability which is directly related to the qualitative increase and decr ... | 1979 | 155387 |
| [antibiotic sensitivity of strains of nonagglutinating vibrios and el tor vibrios]. | | 1979 | 155421 |
| an ultrastructural study of nerve terminal degeneration in muscle spindles of the tenuissimus muscle of the cat. | muscle spindles in the tenuissimus muscle of the cat were studied between 12 and 168 h after cutting or freezing the nerve to this muscle. degenerative changes in sensory and motor nerve terminals on intrafusal muscle fibres were observed using the electron microscope. comparisons were made with spindles from unoperated or sham-operated cats. the earliest degenerative changes were seen in sensory and motor terminals at 20-24 h after the lesion. no nerve endings were seen by 114 h after denervati ... | 1979 | 155729 |
| modifications of the local immune response to vibrio cholerate attributed to the intestinal microbial flora of the mouse. | oral immunisation studies in germfree, specific pathogen-free (spf) and conventionalised mice illustrated that the autochthonous gut flora can have a suppressive effect on the induction of a local intestinal immune response to vibrio cholerae. temporary colonisation of the small bowel by viable vibrios occurred only in the germfree animal. the lack of colonisation in spf and conventionalised mice was presumably a cause of their lower coproantibody responses. prevention of colonisation was probab ... | 1978 | 156020 |
| the suppression of mitogen- and alloantigen-stimulated peripheral blood lymphocytes by cultured human t lymphocytes. | | 1979 | 156232 |
| circular dichroism and ultraviolet absorbance of calf thymus dna in presence of ch3hgoh. | | 1979 | 156470 |
| a method for cloning t-lymphocytic precursors in agar. | a new technique for the culture of t-lymphocytic colonies is reported. the method may be regarded as a human lymphocyte precursor cell assay, as is the myeloid colony culture for granulocyte-macrophage progenitors. the colonies arise under the simultaneous stimulation of phytohemagglutin and a leukocyte feeder. a linear relationship is found between colony numbers and cell numbers plated. the colonies represent aggregates of lymphoblast-like cells, the majority of which are capable of e-rosette ... | 1979 | 156495 |
| changes in the immunoglobulin levels of the mouse gut and serum during conventionalisation and following administration of salmonella typhimurium. | increasess in all immunoglobulin classes, except igm, were observed in the sera of specific pathogen-free (spf) mice beginning 10 days after their removal from barrier conditions. concentrations of serum immunoglobulins, comparable with those of conventional mice, were obtained in these animals between 21 and 35 days. following the removal of germ-free mice from their sterile isolaters, their intestinal iga levels increased over 35 days to attain levels found in conventional animals. a marked in ... | 1978 | 156538 |
| [inhibitory action of some bacterial exoproteases in colicines of enterobacteria (author's transl)]. | | 1979 | 156592 |
| [intestinal antibodies against lipopolysaccharide antigens of nag vibrio in rabbits immunized by the oral route (author's transl)]. | | 1979 | 159131 |
| [neonatal thrombocyte values in children with down's syndrome and other autosome trisomies]. | platelet counts were determined in 70 neonates with trisomy-21, 10 neonates with trisomy-18 and 6 neonates with trisomy-13 during the first days of life. 60% of all infants with trisomy-aberrations were found to have thrombocytopenia. platelet counts in down's syndrome averaged 104600 (sd 53000; median 90500; 10- and 90-percentile at 45000 and 175000) per microliter. a correlation with other hematological features of trisomy-21 was examined. there was no significant correlation between platelet ... | 1979 | 161560 |
| allometric radial growth in muscle, comparing fibres with strong and with weak adenosine triphosphatase activity. | a large flock of male and female white turkeys (meleagris gallopavo) was reared for 22 weeks after hatching. at two weeks intervals small groups of birds were removed from the flock and killed. samples of sartorius muscles were taken from the bird nearest the mean weight for its group. transverse frozen sections were tested for atpase activity by calcium method at ph 9.4. fibres were separated into two categories (strong-atpase and weak-atpase) and their mean minimum diameters were measured with ... | 1979 | 161561 |
| cytotoxic and proliferative lymphocyte responses to allogeneic and xenogeneic antigens in vitro. | in vitro lymphoproliferative responses to foreign histocompatibility antigens are phylogenetically restricted. responses occur most readily to allogeneic or closely related xenogeneic leucocytes, but not to unrelated xenogeneic cells. specific cytotoxic t cell responses to foreign histocompatibility antigens show the same phylogenetic restriction. this lack of xenoreactivity is not due to a lack of precursor cells for the xenoantigens; guinea-pig lymphocytes, although normally unresponsive to mo ... | 1979 | 161967 |
| competition between extramitochondrial and intramitochondrial atp-consuming processes. | the relationship between intra- and extramitochondrial atp utilization was investigated in liver mitochondria isolated from normally fed, starved and high-protein fed rats. atp export was provoked by adding a hexokinase-glucose-trap and intramitochondrial atp consumption by adding ammonia, bicarbonate and ornithine in order to stimulate citrulline synthesis. both processes compete for atp produced via oxidative phosphorylation; the rate of citrulline formation declines as the extramitochondrial ... | 1979 | 162025 |
| 7-substituted theophyllines. part i. nicotinamide derivatives. | some nicotinamides derived from 7-substituted theophyllines were prepared and pharmacologically screened. they showed a very low coronarodilatory activity, a remarkable antispastic activity and a low toxicity. | 1979 | 162286 |
| classification of enterotoxins on the basis of activity in cell culture. | two cell culture systems were used in a study of the biological properties of several bacterial enterotoxins in vitro. by means of one model, in which hela cell monolayers were used, cytotoxic effects, interms of detachment of cells from a glass surface due to cell death, were assayed. by means of the second model, activation of the adenyl cyclase-cyclic adenosine 3', 5'-monophosphate (amp) system, in terms of increased steroidogenesis by y-1 adrenal cells (an effect which we have termed cytoton ... | 1975 | 162926 |
| stimulation of cyclic adenosine 3':5'-monophosphate and corticosterone formation in isolated rat adrenal cells by cholera enterotoxin. comparison with the effects of acth. | 1. the production of cyclic adenosine 3':5'-monophosphate (cyclic amp) and corticosterone isolated ratadrenal cells was increased by cholera enterotoxin. both responses were accompanied by a lag period which is characteristic of other known actions of enterotoxin. the duration of the lag period in the production of corticosterone depended on the concentration of enterotoxin; with the maximally stimulating amounts it was 30-45 min. 2. maximum rates of cyclic amp and corticosterone synthesis, afte ... | 1975 | 163102 |
| adenosine 3',5'-cyclic monophosphate in vibrio cholerae. | the extracellular concentration of cyclic adenosine 3',5'-monophosphate (amp) of three different strains of vibrio cholerae growing in syncase medium were measured. cyclic amp secreted by v. cholerae 569b varied widely, with different carbon sources. mutant 13, which produced little or no toxin, released half the amount of cyclic amp as the wild type. the release of less cyclic amp into the medium by mutant 13 may be accounted for by the lower activity of adenylate cyclase observed. high glucose ... | 1975 | 163219 |
| modulation of in vivo antibody responses by cholera toxin. | treatment of mice with an exotoxin (0.01 mug to 1.0 mug) purified from vibrio cholerae culture filtrates markedly influenced the immune response to sheep erythrocytes (srbc) and the escherichia coli lipopolysaccharide (lps). simultaneous administration of the toxin (ct) with antigen resulted in a delayed appearance of antibody plaque-forming cells (pfc) during the first few days after immunization, followed by a marked enhancement of both igm and igg pfc. the secondary immune response to srbc wa ... | 1975 | 163279 |
| selection of a variant lymphoma cell deficient in adenylate cyclase. | isoproterenol, a stimulator of adenylate cyclase, was used to select a stable variant clone of mouse lymphosarcoma cells deficient in the enzyme. the inability of four different stimulators to activate cyclic adenosine monophosphate synthesis in the variant, in contrast to its wild-type parent, implies that in normal cells one type of adenylate cyclase molecular can respond to different activators. | 1975 | 163487 |
| inactivation of viruses and bacteria by ozone, with and without sonication. | selected organisms with public health significance were placed in a reaction chamber for treatment by ozonation, by ozonation and sonication, by sonication, or by sonication during oxygenation. vesicular stomatitis virus, encephalomyocarditis virus, gdvii virus, staphylococcus aureus, pseudomonas fluorescens, salmonella typhimurium, enteropathogenic escherichia coli, vibrio cholerae, and shigella flexneri were inactivated by treatment with ozone. when microorganisms were suspended in phosphate-b ... | 1975 | 163616 |
| structural studies on carcinoembryonic antigen. periodate oxidation. | periodate oxidation has been applied to examine the carbohydrate structure of carcinoembryonic antigen (cea) and the possible role of the carbohydrate residues in its antigenic activity. sialic acid (n-acetylneuraminic acid) and fucose were completely destroyed, and galactose and mannose were partially destroyed by a single periodate treatment. serial periodate treatment (smith degradation) destroyed additional amounts of galactose and mannose as well as significant amounts of n-acetylglucosamin ... | 1975 | 163645 |
| antigenic relationship between medullary thymocytes and a subpopulation of peripheral t cells in the rat: description of a masked antigen. | | 1975 | 163704 |
| immunological comparison of various human pregnancy-associated plasma proteins. | direct immunodiffusion comparison with specific antisera demonstrated that all of the four pregnancy-associated plasma proteins (papps) described in our laboratory are distinct from the pregnancy zone protein (von schoultz); alpha2-pregnoglobulin (berne); pregnancy-associated alpha2-glycoprotein (sp3) (bohn); new serum alpha2-macroglobulin (stimson); pag (horne); pregnancy-associated alpha2-globulin (kasukawa); pal (mclaren), and xh protein (dunston). all the latter proved to be immunologically ... | 1975 | 163802 |
| regulation of the in vitro early anamnestic antibody response by exogenous cholera enterotoxin and cyclic amp. | keyhole limpet hemocyanin was injected into the hind foot pads of rabbits. six days later cell suspensions were prepared from the popliteal lymph nodes. various amounts of hemocyanin (1 ng to 100 mug) were added to 1 times 10-7 cells to induce an anamnestic antibody response. various amounts of cholera enterotoxin, which stimulates the enzyme adenylate cyclase, or dibutyryl cyclic adenosine 3'5'-monophosphate (amp), were added to the cultures with or without hemocyanin. de novo synthesis of anti ... | 1975 | 163858 |
| mechanism of action of cholera toxin and the mobile receptor theory of hormone receptor-adenylate cyclase interactions. | rat liver membrane adenylate cyclase (ec 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. choleragen similarly increases the sensitivity of cyclase to other peptide (acth, vasoactive intestinal polypeptide) and nonpeptide (catecholamines) hormones in this and other tissues. the rate of 125i-labeled glucagon-membrane dissociation is decreased about 2-fold in toxin-treated liver membranes. toxin-activated ... | 1975 | 164020 |
| effects of proteases and neuraminidase on rbc surface charge and agglutination. a kinetic study. | electrophoretic mobility, membrane sialic acid content and agglutinability by "incomplete" antisera against rh-o, hr' and k antigens were determined for red blood cells in the course of treatment with trypsin, ficin and neuraminidase. neuraminidase gradually produces a slight to moderate agglutinability as it reduced surface charge density in proportion to the amount of sialic acid removed. proteases acted in two distinct steps. the first stage is characterized by the cells rapidly becoming high ... | 1975 | 164087 |
| in vitro stimulation of steroidogenesis in rat testis by cholera enterotoxin. | | 1975 | 164187 |
| the biogenesis of mitochondrial membranes in the yeast saccharomyces cerevisiae. | membrane lipids of yeast mitochondria have been enriched by growing yeast cells in minimal medium supplemented with specific unsaturated fatty acids as the sole lipid supplement. using the activity of marker enzymes for the outer (kynurenine hydroxylase) and inner (cytochrome c oxidase and oligomycin-sensitive atpase) mitochondrial membranes, arrhenius plots have been constructed using both promitochondria and mitochondria obtained from o2-adapting cells in the presence of a second unsaturated f ... | 1975 | 164216 |
| kinetics of glycerol uptake by the perfused rat liver. membrane transport, phosphorylation and effect on nad redox level. | the kinetics of glycerol uptake by the perfused rat liver were determined according to a model which includes membrane transport, intracellular phosphorylation and competitive inhibition of glycerol phosphorylation by l-glycerol 3-phosphate. the membrane transport obeys first-order kinetics at concentrations below 10 mm in the affluent medium. the k-m of the glycerol phosphorylation was 10 mum and the k-i of the l-glycerol 3-phosphate inhibition was 50 mum. the maximum activity (v) was 3.70 mum ... | 1975 | 164217 |
| cyclic 3',5'-amp phosphodiesterase of rabbit aorta. | cyclic amp and cyclic gmp phosphodiesterase activities (3' : 5'-cyclic amp 5'-nucleotidohydrolase, ec 3.1.4.17) were demonstrated in the isolated intima, media, and adventitia of rabbit aorta. the activity for cyclic amp hydrolysis in the intima was 2.7-fold higher than that for cyclic gmp hydrolysis. the activity for cyclic amp hydrolysis in the media was approximately equal to that for cyclic gmp hydrolysis, but in the adventitia, cyclic gmp hydrolytic activity was 2.1-fold higher than cyclic ... | 1975 | 164219 |
| aspects of hepatic hypoxia: observations on the isolated, perfused pig liver. | | 1975 | 164256 |
| lanthanum inhibition of vibrio cholerae and escherichia coli enterotoxin-induced enterosorption and its effects on intestinal mucosa cyclic adenosine 3',5'-monophosphate and cyclic guanosine 3',5'-monophosphate levels. | several trivalent cations, including lanthanum (la3+), inhibited the secretion (enterosorption) induced by the enterotoxins of vibrio cholerae and escherichia coli in the rabbit ileum in vivo. high concentrations (greater than 10 mm) of la3+ were required to inhibit cholera enterotoxin (ce)-induced enterosorption, probably because of the adsorption of the la3+ often potentiated the ce-induced enterosorption. if luminal la3+ exposure followed ce exposure, some recovery of the enterosorptive respo ... | 1975 | 164410 |
| specific susceptibility of sensitized (memory) b cells to suppression and antigenic alteration by murine leukemia virus. | mice infected with murine oncogenic rna viruses, either friend virus (fv) or moloney leukemia virus (mulv-m) reacted well to a primary immunization with antigen(s) of vibrio cholerae; both the kinetics and magnitude of the direct (igm) primary vibriolytic antibody plaque forming cell (pfc) response in the spleen was the same as in non-infected control mice. in contrast, the anamnestic pfc (igm) response of mice preimmunized with cholera and later (2 to 9 months following priming) infected with e ... | 1975 | 164504 |
| lymphocyte depletion induced by cholera toxin; relationship to adrenal cortical function. | intravenous injection of cholera toxin (choleragen) into mice caused a profound lymphocytopenia associated with marked cellular depletion of the lymph nodes, spleen, and thymus. after administration of 1 mu g of choleragen, lymphocytopenia was mot marked at 24 hr; recovery occurred 6 to 10 days later. similarly depletion of lymph nodes and spleen was maximal at 24 hr with recovery by 14 days. choleragen also caused a marked elevation of serum corticosterone and lymphocyte depletion was not obser ... | 1975 | 164505 |
| human very low density lipoproteins: loss of electrophoretic mobility on enzymatic removal of sialic acid residues. | incubation of very low density lipoproteins (vldl) from three hyperlipoproteinemic patients with neuraminidase resulted in a progressive liberation of sialic acid residues. after the original sialic content had been reduced by approx. 75% a lipoprotein fraction having zero electrophoretic mobility on paper appeared. this fraction increased until, after the removal of all the sialic acid residues, the mobility of the entire vldl fraction was reduced to zero. | 1975 | 165020 |
| immune response of cattle to intranasally or parenterally administered parainfluenza type 3 virus vaccines. | the principal significance of pi-3 virus in the bovine respiratory disease complex would seem to be its role in predisposing the respiratory tract to bacterial infection by (5) disrupting the integrity of the superficial mucosa and (3) producing a more favourable milieu for bacterial growth. attention has therefore been given to establishing local immunity to preclude infection of superficial cells of the mucosa. criteria used to evaluate the degree of such immunity are (5) presence of specific ... | 1975 | 165124 |
| local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3. | triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ... | 1975 | 165126 |
| organ cultures of rat and hamster colon. | human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. explants of rat and hamster colon, maintained in leibovitz's l-15 medium buffered with hepes in the presence of 5% co2 and air, retained their normal columnar epithelial architecture for 24 days in culture. outgrowth of epithelial cells from colon ... | 1975 | 165141 |
| non-producer human cells induced by murine sarcoma virus. | non-produces (np) human cells were isolated from transformed foci induced by the kirsten mouse sarcoma virus. these morphologically altered np cells produced neither infectious virus nor complement-fixing antigens of the murine sarcoma-leukemia virus complex. however, the sarcoma virus genome could be rescued from these nf cells by co-cultivation with cells carrying "helper" kirsten mouse leukemia virus or woolly monkey leukemia virus. the possible usefulness of these cells in efforts designed t ... | 1975 | 165148 |
| hydrophobic interactions of the apo-gln-i polypeptide component of human high density serum lipoprotein. | apo-gln-i, the major polypeptide component of human serum high density lipoprotein, has four noninteracting hydrophobic sites which associate with alkanes, anionic detergents, and cationic detergents. hexane and octane bind to these sites with association constants of 6.8 times 10-2 and 1.8 times 10-4 liters/mol, respectively, and compete with the anionic detergent, sodium dodecyl sulfate (c12oso3-minus), at low detergent ligand binding ratios (i.e. smaller than or equal to 1.0 mol of c12oso3-mi ... | 1975 | 165177 |
| enterotoxins of escherichia coli and vibriocholerae: tools for the molecular biologist. | with the independent discovery in several different laboratories that vibrio cholerae and heat-labile escherichia coli enterotoxins activated an enzyme (adenylate cyclase) in small intestinal epithealial cells to cause an enhanced intestinal secretion mediated by cyclic adenosine 3',5'-monophosphate (cyclic amp), a molecular mechanism was provided for these disease states. as agents that also elevate intracellular concentrations of cyclic amp in virtually every mammalian tissue tested, the enter ... | 1975 | 165247 |
| long-term primary culture of mouse mammary tumor cells: production of virus. | long-term primary cultures of mouse mammary tumor cells proved an excellent source of mouse mammary tumor virus (mmtv). virus purified from these primary cultures had the same morphologic biochemical, immunologic, and biologic characteristics as mmtv. quantitation of mmtv-protein equivalents released into the medium was measured by the radioimmunoassay for mmtv. peak production levels were 20-40 mug mmtv protien equivalents/75-cm-2 flask/24 hours. these cultures produced mmtv for as long as 90 ... | 1975 | 165313 |
| [biochemical characteristics of nucleic acid decomposing enzymes of the epidermis]. | | 1975 | 165330 |
| assessment of in vivo adrenocorticotropic hormone treatment on adrenal mitochondrial functions in vitro. | | 1975 | 165376 |
| rhythmic and phytochrome-regulated changes in transmembrane potential in samanea pulvini. | | 1975 | 165432 |
| inhibition of fc receptors on a murine lymphoid cell line by cholera exotoxin. | | 1975 | 165433 |
| letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy? | | 1975 | 165451 |
| a simple radioimmunoassay for 3',5', cyclic adenosine monophosphate. | | 1975 | 165745 |
| actions of a new muscle relaxant (ah8165) on neuromuscular transmission. | the effects of a new muscle relaxant, ah8165, on miniature endplate potential (mepp) amplitude and frequency, endplate sensitivity to acetylcholine, and muscle twitch tension were studied in vitro in the frog sartorius muscle. nerve terminal effects were studied in vivo in the cat soleus muscle and its ventral root fibers. ah8165 stimulates the nerve terminal, as evidenced by increased mepp frequency and the appearance of post-drug repetitive activity. in the same concentration range at which me ... | 1975 | 165756 |
| the isolation of thymopoietin (thymin). | the isolation from bovine thymus of two closely related polypeptides, thymopoietin i and ii, is described. these are considered to be thymic hormones, which physiologically induce the differentation of prothymocytes to thymocytes within the thymus. ths isolation of the thymopoietins was monitored not by their differentiative effects, but by a presumably secondary effect on neuromuscular transmission. this was discerned in experimental studies related to the human disease myasthenia gravis in whi ... | 1975 | 165765 |
| respiratory virus infections in children hospitalized in montreal, 1968 - 1972. | | 1975 | 165872 |
| biochemical and morphological changes in the subcellular fractions during myelination of rat brain. | | 1975 | 165992 |
| in-vitro activity of methronidazole on the cysts of entamoeba histolytica. | | 1975 | 166039 |
| cyclic amp metabolism and nucleic acid content in the lymphocytes of the thymus, spleen, and lymph nodes of protein-deficient rats. | the concentration of cyclic amp (camp) and its metabolites (5'-amp and adenosine) as well as the adenyl cyclase, camp phosphodiesterase, and 5'-nucleotidase activities were determined in lymphocytes of thymus, spleen, and lymph nodes of control and protein-deficient rats. the values of these parameters, when expressed as per milligran dna and as per 10-8 cells, but not always when expressed as per milligran protein, were much lower in the thymus as compared with the spleen and the lymph nodes in ... | 1975 | 166150 |
| dexamethasone-binding receptor in bovine pituitary cytosol. | | 1975 | 166255 |
| control of arrhythmias following ligation of the circumflex artery. | in experimental work, three groups of 10 dogs each were subjected to ligation of the circumflex coronary artery. one group was given prophylactic medication with procainamide and another group was allowed a 2 hour interval between thoracotomy and ligation. the effects of these measures on the incidence of ventricular arrhythmias during the 30 minutes immediately after coronary artery occlusion were then compared. the two groups of animals on which these preventive measures were tested both showe ... | 1975 | 166259 |
| involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro. | nad is a necessary cofactor for the activation of adenylate cyclase (atp pyrophosphate-lyase (cyclizing), ec 4.6.1.1) by cholera toxin. lysates of certain types of cell that hydrolyze their endogenous store of nad after cell disruption respond poorly or not at all to cholera toxin. lysates of pigeon erythrocytes, which lack enzymes that degrade nad, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action ... | 1975 | 166378 |
| isolation of a glycogen synthase i kinase that is independent of adenosine 3':5'-monophosphate. | three protein kinases (atp:protein phosphotransferase, ec 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on deae-cellulose with a linear nac1 gradient. the first two kinases eluted (peak 1 and peak ii) were cyclic-amp-dependent, wheras peak iii was cyclic-amp-independent. a procedure was developed to separate the catalytic subunit of peak ii cyclic-amp-dependent protein kinase (representing the bulk of the histone kinase activity) from peak iii pro ... | 1975 | 166380 |
| [rna involvement in dna replication (author's transl)]. | | 1975 | 166414 |
| pleiotropic effects of a dna adenine methylation mutation (dam-3) in escherichia coli k12. | the dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-mea) residues in the dna of e. coli k12 or phage lambda. the dna of phage fd appears to be devoid of 6-mea when propagated on dam-3 bacteria. the phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (mms), (3) inviability of dam-3 lex-i strains, (4) lower molecular weight of dna in dam-3 bact ... | 1975 | 167279 |
| wound infection during the yom kippur war: observations concerning antibiotic prophylaxis and therapy. | eighty-eight episodes of wound associated infection were identified among 624 consecutively admitted battlefield casualties. ninety-one per cent of infections occurred during the administration of antibiotic therapy or prophylaxis and 65% were associated with the use of multiple antibacterial agents. gram negative bacillary and mixed microbial infection predominated and were found to increase in relative incidence after the second day of hospitalization. appropriate therapy, based on disc sensit ... | 1975 | 167666 |
| stimulation of late interphase dictyostelium discoideum amoebae with an external cyclic amp signal. | the microelectrode system described in the accompanying paper was used to investigate properties of fields of dictyostelium discoideum amoebae in late interphase. cells in the fields were competent to respond chemotactically to, and to relay, a c-amp signal, but not to produce an aggregative signal autonomously. the experimental results are generally consistent with c-amp being the sole compound required for chemotaxis and signal relaying. a periodic signal from the microelectrode can initiate a ... | 1975 | 167879 |
| adrenal cells in tissue culture the effects of choleragen and acth on steroid and cyclic-amp metabolism. | primary cultures of mouse adrenocortical tumors provide a sensitive system for investigating the effects of the enterotoxin of the v. cholerae (choleragen) on cyclic-amp metabolism in the intact cell. like acth, the toxin stimulates the synthesis and release of steroids from these cells but its mode of action differs from that of acth. the steroidogenic response to acth is immediate and of limited duration. the initial rate of steroidogenesis is the highest. in contrast, the steroidogenic respon ... | 1975 | 168056 |
| the action of vibrio cholerae and corynebacterium diphtheriae neuraminidases on the sendai virus receptor of human erythrocytes. | studies of the sendai virus haemagglutinin receptor on the human erythrocyte surface have confirmed that it involves 2 leads to 3 linked sialic acid. because the primary specificity of vibrio cholerae neuraminidase is for this linkage, it is able to compete with the virus for the receptor, to which it adsorbs strongly at low temperatures. corynebacterium diphtheriae neuraminidase, whose principal specificity is for a sialic acid linkage other than 2 leads to 3, does not easily remove sendai viru ... | 1975 | 168311 |
| antibody levels to parainfluenza, rubella, measles, and influenza a virus in children with polymyositis. | the cf and hi antibody titers to rubella and measles viruses, the cf titers to influenxa a, and the hi titers to parainfluenza 1, 2, and 3 were carried out on the sera of 20 patients with childhood polymyositis and their matched controls. the titers for measles, parainfluenxa 1, and influenza a were slightly higher for patients than for controls. the control group had antibody titers to rubella and parainfluenza 2 and 3 higher than or similar to those of patients. strong patterns or significant ... | 1975 | 168906 |
| characterization of cloned evolutionary variants of simian virus 40. | | 1975 | 169050 |
| polyoma virus--a study of wild-type, mutant and defective dnas. | | 1975 | 169088 |
| fetal calf serum alters cyclic adenosine 3',5'-monophosphate's effect on heme synthesis in vitro. | an investigation of the effect of camp on heme synthesis of rat bone marrow cells revealed that at 10(-2) m this cyclic nucleotide inhibits heme synthesis and that optimum stimulation occurs at 10(-4) m. some unidentified constituent of fetal calf serum in the culture medium modifies the direction and degree of camp's effect. | 1975 | 169145 |
| the differential effect of cholera toxin on the lymphocyte stimulation induced by various mitogens. | balb/c spleen cells (5 x 10(6)) were cultured in 1 ml of serum-free rpmi 1640 medium for 3 days in order to examine the effect of cholera enterotoxin (cn) and its spontaneously formed toxoid (cd) on lymphocyte stimulation. stimulation was assessed after addition of [3h] thymidine for the last 16 hours of culture. one microgram of cn per culture markedly reduced the baseline of [3h] thymidine incorporation and the stimulation due to phytohaemagglutinin (pha), concanavalin a (con a) and bacterial ... | 1975 | 169198 |
| effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat. | the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ... | 1975 | 169297 |
| recent developments in immunization against diarrheal diseases. | | 1975 | 169592 |
| functional consequences of modifying highly reactive arginyl residues of fructose 1,6-bisphosphatase. loss of monovalent cation activation. | modification of pig kidney fructose 1,6-bisphosphatase with 2,3-butanedione (in the presence of amp) results in the loss of activation of the enzyme by monovalent cations. under these conditions about 8 arginyl residues per mole of enzyme were modified. no other residues were modified. no loss of monovalent cation activation occurs when modification with 2,3-butanedione is carried out in the presence of amp plus the substrate fructose 1,6-bisphosphate and 3.2 less arginyl residues were modified. ... | 1975 | 169892 |
| 2-chloroadenosine-dependent elevation of adenosine 3',5'-cyclic monophosphate levels in rat caudate nucleus slices. | | 1975 | 169954 |
| ultrastructure of human bronchiolo-alveolar cell carcinoma. | ultrastructural features were correlated with a series of special staining reactions in eight cases of bronchiolo-alveolar carcinoma. ultrastructural, all tumors were similarly composed of large cells with abundant cytoplasm and small nuclei in close contact with each other. straight membranes or complex interdigitations occurred within adjacent tumor cells, attached to each other by scattered desmosomes. microvilli or cilia abutted from free surfaces of the cells, and were noted in different st ... | 1975 | 169987 |
| genetic control of oncogenesis by murine sarcoma virus moloney pseudotype. ii. a dominant epistatic susceptibility gene. | we have shown in the preceding paper that akr mice are highly resistant to m-msv tumor development, and that resistance is transmitted as a dominant character. in the present studies the tumor-response pattern of f1 hybrids between resistant akr and susceptible strains (c57bl/6, balb/c and b10br) following injection with moloney mouse sarcoma virus (m-msv) resembles that of the non-akr parent. segregation is observed in first backcross (bc1) and f2 mice, and the segregation ratios up to bc3 mice ... | 1975 | 170220 |
| mode of action of sodium nitroprusside on vascular smooth muscle. | 1. sodium nitroprusside is a potent relaxant of smooth muscles with a predominantly tonic response, e.g. rat aorta contracted by noradrenaline, angiotensin ii, phe2-lys8-vasopressin, bac1(2), or kc1, and guinea-pig tracheal smooth muscle contracted by carbachol. 2. smooth muscle preparations from the splanchnic region and with varying degrees of phasic contractility are less sensitive and develop tachyphylaxis (portal vein, duodenum of the rat) or are unresponsive to sodium nitroprusside (vas de ... | 1975 | 170545 |