| determinants for thermoinducible cell binding and plasmid-encoded cellular penetration detected in the absence of the yersinia pseudotuberculosis invasin protein. | yersinia pseudotuberculosis inv mutants were analyzed for their ability to bind and penetrate mammalian cell lines. strains defective for the production of invasin and cured of the yersinia virulence plasmid pib1 were extremely defective for entry into the hep-2 cell line. inv strains harboring the virulence plasmid partially overcame this defect, indicating that the virulence plasmid mediates an invasin-independent pathway for low-level entry into cultured cells. plasmid-cured inv mutants were ... | 1989 | 2543628 |
| differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats. | ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ... | 1989 | 2546615 |
| demonstration of monoclonal ebv genomes in hodgkin's disease and ki-1-positive anaplastic large cell lymphoma by combined southern blot and in situ hybridization. | forty-two cases of hodgkin's disease (hd) and 22 cases of ki-1-positive anaplastic large cell (ki-1 + alc) lymphoma were examined by southern blotting for the presence of epstein-barr virus (ebv) dna. seven cases of hd and one case of ki-1 + alc lymphoma scored positive with a probe specific for the internal repetitive region of the ebv genome. subsequently, these viral genomes could be demonstrated to be monoclonal in origin using an ebv terminal region dna probe. in situ hybridization revealed ... | 1989 | 2546633 |
| regulation of hepatic expression of igf i and fetal igf binding protein mrna in streptozotocin-diabetic rats. | hepatic mrna levels of insulin-like growth factor i (igf i) and of the fetal, nonglycosylated 32 kda igf-binding protein (bp) were analysed in diabetic, diabetic insulin- and igf i-treated rats as well as in age-matched, healthy control animals. igf imrna levels are reduced in diabetic rats and increased by insulin treatment. in contrast, the infusion of igf i does not significantly upregulate igf i mrna levels. fetal igf bp mrna expression is very low in healthy control animals, but high levels ... | 1989 | 2546824 |
| the e6 and e7 genes of hpv-18 are sufficient for inducing two-stage in vitro transformation of human keratinocytes. | using a recently described keratinocyte assay, we demonstrate that hpv-18 dna induces two progressive steps in cellular transformation (a large cell and a small cell stage). both steps of this keratinocyte transformation can be achieved with a subgenomic fragment containing only the hpv-18 regulatory region and e6/e7 genes. similar to cell lines transformed by the complete hpv-18 genome, keratinocytes transformed by the hpv-18 e6/e7 genes express the major early viral protein (e7) but are non-tu ... | 1989 | 2556677 |
| characterization of l-glutamate action on the release of endogenous dopamine from the rat caudate-putamen. | in the present study the effect of l-glutamic acid (l-glu), n-methyl-d-aspartic acid (nmda), kainic acid (ka) and quisqualic acid (quis) on the release of endogenous dopamine (da) from slices of the rat caudate-putamen was investigated. da was measured by high-performance liquid chromatography coupled to an electrochemical detector. l-glu, nmda, ka and quis, in the absence of mg++, produced a dose-related, ca++-dependent increase in da release. the order of agonist efficacy was l-glu greater tha ... | 1989 | 2563769 |
| the effect of some tricyclic antidepressants on the inhibition of mouse brain monoamine oxidase in-vivo by phenelzine. | four tricyclic antidepressants, amitriptyline, imipramine, desipramine and iprindole have been shown to partially protect mouse brain monoamine oxidase in-vivo from the irreversible enzyme inhibition produced by subsequent injection of phenelzine. levels of protection were similar when the enzyme was assayed with selective substrates (5-hydroxytryptamine and phenethylamine) for both the a and b forms of the enzyme. although other explanations cannot at this stage be ruled out, these observations ... | 1989 | 2565965 |
| incidence and sonographic diagnosis of bacterial ileocaecitis masquerading as appendicitis. | 533 consecutive patients with suspected acute appendicitis or appendiceal mass were examined by ultrasonography to distinguish acute appendicitis from bacterial enteritis. in 61 (11.4%) ultrasonography revealed the characteristic picture associated with bacterial enteritis of the ileocaecal region--enlarged mesenteric lymph nodes and mural thickening of the terminal ileum and caecum--but no image of the appendix. in 41 of these a bacterial infection was confirmed: infection due to yersinia enter ... | 1989 | 2567879 |
| on the mechanism of positive inotropic effects of alpha-adrenoceptor agonists. | the positive inotropic effect of the alpha 1-adrenoceptor agonist phenylephrine is accompanied by an increase in the presumed second messengers inositol 1,4,5-trisphosphate (1,4,5-ip3) and inositol 1,3,4,5-tetrakisphosphate (1,3,4,5-ip4). both 1,4,5-ip3 and 1,3,4,5-ip4 sensitize myocardial contractile proteins in chemically skinned fibers. in addition to the ca++ releasing effect of 1,4,5-ip3 from the sarcoplasmic reticulum the ca++-sensitizing effect of the inositol phosphates may play a role i ... | 1989 | 2573342 |
| structure-activity relationships as a response to the pharmacological differences in beta-receptor ligands. | with a few notable exceptions, beta-receptor ligands (agonists and antagonists) belong to the aryl- or heteroaryl-ethanolamine series and to the aryl- or heteroaryl-oxypropanolamine series. structure-activity relationships for beta-adrenergic agonists show that a secondary amine in the phenylethanolamine side chain ending is essential for receptor stimulation. the 3,4-dihydroxyphenyl groups may be replaced by "phenol equivalents" (-ch2oh, -nhconh2, -choh, -nhso2ch3). in contrast, substitution at ... | 1989 | 2573372 |
| long-range mapping in the research and diagnosis of genetic disease. | this paper reviews current genetic and molecular biological methods that may be used in the so-called "reverse genetics" approach. these methods are the mapping, isolation, and study of the chromosomal dna containing a previously unidentified gene responsible for a genetic disease, beginning with its chromosomal localization. in principle, the reverse genetics methodology follows the same path for different diseases studied. an overall outline of the steps to be undertaken is given and discussed ... | 1989 | 2576651 |
| autoradiographic detection of hprt variants of human lymphocytes resistant to rna synthesis inhibition. | the feasibility of using rna synthesis in freshly isolated, human peripheral blood lymphocytes to detect 6-thioguanine (tg)- and 8-azaguanine (ag)-resistant variants in an autoradiographic assay similar to that of strauss and albertini (1979) has been evaluated. in phytohemagglutinin (pha)-stimulated cultures rna synthesis and hprt activity began well in advance of dna synthesis and increased in parallel during the first 44 h of culture. introduction of tg or ag with pha at the beginning of cult ... | 1985 | 2579332 |
| [use of pseudotuberculosis coagglutinating antibody diagnostic agent in laboratory practice]. | | 1985 | 2581018 |
| [the significance of detecting corynebacterium equi in the conjunctiva of children in cairo]. | the probable correlations between certain corynebacterium are discussed. carpenter et al. (1976) describes a corynebacterium equi-pneumonia in a patient with hodgkin's disease; gardner et. al. (1976) also reports on a case of pneumonia. golub et. al. (1967) demonstrates a lung abscess resulting from corynebacterium equi. williams et. al. (1971) demonstrates a thoracic infection in an immunosuppressed patient. although, our findings have been demonstrated only in conjunctivitis of children, hithe ... | 1989 | 2583507 |
| comparison of albendazole, mebendazole and praziquantel chemotherapy of echinococcus multilocularis in a gerbil model. | the efficacy of albendazole (50 mg/kg/d), mebendazole (50 mg/kg/d) and praziquantel (500 mg/kg/d) against established intraperitoneal infections of echinococcus multilocularis in gerbils was compared by monitoring parasite weight and making ultrastructural observations on treated and untreated material. praziquantel was the most active protoscolicidal agent, reducing protoscolex viability to less than 2%, although it did not inhibit cyst growth. albendazole was the most effective agent in reduci ... | 1989 | 2583567 |
| the effect on fetal growth of protozoan and helminthic infection during pregnancy. | this is a prospective study of 14,914 pregnant women conducted in guatemala city, guatemala. stool samples were obtained from the studied patients before the first prenatal visit (mean gestational age 21.6 +/- 8.4 weeks) for the diagnosis of parasitic infections during pregnancy. forty-four percent had at least one parasite detected, and 24% were infected with helminths. ascaris lumbricoides was the most prevalent (14.5%). infected mothers were less educated, had less adequate water and sanitary ... | 1989 | 2586958 |
| the effect on fetal growth of protozoan and helminthic infection during pregnancy. | this is a prospective study of 14,914 pregnant women conducted in guatemala city, guatemala. stool samples were obtained from the studied patients before the first prenatal visit (mean gestational age 21.6 +/- 8.4 weeks) for the diagnosis of parasitic infections during pregnancy. forty-four percent had at least one parasite detected, and 24% were infected with helminths. ascaris lumbricoides was the most prevalent (14.5%). infected mothers were less educated, had less adequate water and sanitary ... | 1989 | 2586958 |
| binding to collagen by yersinia enterocolitica and yersinia pseudotuberculosis: evidence for yopa-mediated and chromosomally encoded mechanisms. | binding of yersinia enterocolitica and yersinia pseudotuberculosis strains to type i, ii, and iv collagens has been studied. wild-type strains which harbored the 40- to 50-megadalton virulence plasmid specifically bound all three types of collagen. curing of the virulence plasmid or tn5 insertion in the yopa gene encoding the temperature-inducible outer membrane protein yop1 abolished the binding of all three collagen types to y. enterocolitica and type i and ii collagens to y. pseudotuberculosi ... | 1989 | 2592347 |
| recovery of proteins and peptides with nanogram loads on non-porous packings. | | 1989 | 2592499 |
| [dna analysis for the kininogen gene of patients with kininogen deficiency in japan]. | the kininogen (kgn) gene status was examined in 4 families with both high molecular weight (hmw) and low molecular weight (lmw) kgm deficiency and one family with only hmw-kgn deficiency reported in japan. no abnormal hmw-kgn or lmw-kgn was detected in those with these deficiencies by immunoblot analysis using monoclonal antibodies (hkg-h12, hkg-l7, hkg-l17) for human hmw-kgn. hmw-dna prepared from peripheral blood leucocytes was digested with endonuclease, ecori, bam hi, hind iii, sca i, bg1ii, ... | 1989 | 2593235 |
| monoclonal antibodies reacting specifically with francisella sp. | twenty two hybridoma strains producing monoclonal antibodies against francisella tularensis atcc 6223, var. tularensis, were characterized. in an enzyme-linked-immunosorbent-assay (elisa) using formaldehyde fixed bacteria as antigens, neither cross-reactions with six different brucella spp., with yersinia enterocolitica 0:9 nor with two biotypes of yersinia pseudotuberculosis could be detected. the antibodies gave comparable titres with the three strains of f.tularensis tested. elisa binding stu ... | 1989 | 2596200 |
| secondary structure of a core protein from pig skin proteodermatan sulfate: cd and fourier transform ir spectroscopic studies in solution. | the secondary structure of a 38 kda core protein from pig skin proteodermatan sulfate (pds), was investigated in solution using cd and fourier transform (ft) ir spectroscopy. both techniques generally have provided complementary data on the secondary structures of proteins. cd spectral analysis has shown that the core protein contains 60% beta-turn and alpha-helical structures, the rest being "unordered" structure. ft ir data do not permit calculation of quantitative contributions of substructur ... | 1989 | 2597740 |
| differential blocking effects of atropine and gallamine on negative chronotropic and dromotropic responses to vagus stimulation in anesthetized dogs. | the negative dromotropic response to cervical vagus stimulation was inhibited less than the negative chronotropic response to cervical vagus stimulation by the same dose of atropine in neurally decentralized, anesthetized dogs. atropine and gallamine bind different muscarinic binding sites. we thus investigated the blocking effects of atropine and gallamine on the negative chronotropic and dromotropic responses to stimulation of the vagus nerves. we stimulated the intracardiac vagus nerves to th ... | 1989 | 2600814 |
| studies on acute toxic effects to keratinocytes induced by hematoporphyrin derivatives and laser light. | human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (hpds) used in photodynamic therapy was estimated. keratinocytes loaded with hpds were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion test and the measurement of enzyme release. with increasing intracellular concentration of hpds and with increasing intensity of the laser light, an increasing number of cells took u ... | 1989 | 2610520 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| separation of iodothyronines from iodinated metabolites in serum from reverse t3 tracer kinetic studies in man and dog: a comparison of two methods. | iodothyronine separation from free iodide and iodoalbumin in serial serum samples obtained from 7 human and 5 dog studies following intravenous injection of radiolabeled reverse triiodothyronine (reverse t3) was compared using acidified ammonium acetate/tetrahydrofuran (thf) elution from c-18 sep-pak cartridges or ethyl acetate/butanol (eab) extraction. both methods excluded greater than 98% free iodide and greater than 99% iodoalbumin from the iodothyronine fraction. recovery of labeled reverse ... | 1989 | 2613183 |
| autosomal dominant retinitis pigmentosa (adrp): localization of an adrp gene to the long arm of chromosome 3. | members of a large pedigree of irish origin presenting with early onset type i autosomal dominant retinitis pigmentosa (adrp) have been typed for d3s47 (c17), a polymorphic marker from the long arm of chromosome 3. significant, tight linkage of adrp to d3s47, with a lod score of 14.7 maximizing at 0.00 recombination, has been obtained, hence localizing the adrp gene (rp1) segregating in this pedigree to 3q. | 1989 | 2613244 |
| primary structure of a ribonuclease from bullfrog (rana catesbeiana) liver. | a pyrimidine base-specific ribonuclease was purified from bullfrog (rana catesbeiana) liver by means of cm-cellulose column chromatography and affinity chromatography on heparin-sepharose cl-6b, which gave single band on sds-slab electrophoresis. the primary structure of the bullfrog liver rnase was determined. it consisted of 111 amino acid residues, including 8 half-cystine residues. from the sequence, it was concluded that three disulfide bridges in rnase a were conserved in the bullfrog rnas ... | 1989 | 2613682 |
| [genetic analysis of fertility of male mice with various t-haplotypes]. | fertility of 47 mouse males carrying various combinations of lethal, t-haplotypes (t6/tw18, t12/tw18, tw73/tw12, tw5/tw18, t6/dt5, t12/tw12, tw5/twpa-1, tw18/twpa-1, tw5/tw12) was studied in crosses with females of different genotypes. the t-haplotypes studied belong to 7 main groups of complementation. the presence of at least two factors of fertility in the t-complex was revealed. the influence of female genotype on the degree of male fertility was also demonstrated. the data presented confirm ... | 1989 | 2620811 |
| [genetic analysis of fertility of male mice with various t-haplotypes]. | fertility of 47 mouse males carrying various combinations of lethal, t-haplotypes (t6/tw18, t12/tw18, tw73/tw12, tw5/tw18, t6/dt5, t12/tw12, tw5/twpa-1, tw18/twpa-1, tw5/tw12) was studied in crosses with females of different genotypes. the t-haplotypes studied belong to 7 main groups of complementation. the presence of at least two factors of fertility in the t-complex was revealed. the influence of female genotype on the degree of male fertility was also demonstrated. the data presented confirm ... | 1989 | 2620811 |
| [the in vitro multiplication kinetics of yersinia pseudotuberculosis--the effect of rhamnose, raffinose and sodium succinate]. | the reproductive potentials of strains yersinia pseudotuberculosis serovars i, ii, iii, iv and v cultivated at different temperatures on media containing rhamnose, raffinose and sodium succinate were examined comparatively. it was established that the growth of serovars i, ii and iii was activated by sodium succinate at temperatures 4 degrees and 25 degrees c, while serovars iv and v showed lower growth rate regardless of the substrate used. at 37 degrees c d-raffinose activated the growth of th ... | 1989 | 2624161 |
| growth and survival of four strains of francisella tularensis in a rich medium preconditioned with acanthamoeba palestinensis. | the hypothesis of positive interactions between francisella tularensis lvs (live vaccine strain) and acanthamoeba palestinensis was tested. pregrowth of the amoebae, in a rich (autoclaved and filtered) medium, from which they were subsequently removed by filtration, conditioned the medium so that growth of the live vaccine strain of f. tularensis occurred and the growth rate of one other strain was increased. | 1989 | 2630032 |
| isopenicillin n synthase and desacetoxycephalosporin c synthase activities during defined medium fermentations of streptomyces clavuligerus: effect of oxygen and iron supplements. | when the level of dissolved oxygen was increased to saturation in defined media fermentations of streptomyces clavuligerus, the total duration of activity of the penicillin ring cyclization enzyme, isopenicillin n synthase (ipns), was extended by at least 20 h; however, no increase in the stability of the ring expansion enzyme, desacetoxycephalosporin c synthase (daocs), was observed. consequently, the conversion of the excreted intermediate penicillin n to cephamycin c was 15-20% less efficient ... | 1989 | 2630033 |
| effect of the calcium-binding protein regucalcin on the ca2+ transport system in rat liver microsomes: the protein stimulates ca2+ release. | the effect of the calcium-binding protein regucalcin on the ca2+ transport system in the liver microsomes from fed rats was investigated. ca2+ transport was assayed by the method of millipore filtration to estimate microsomal 45ca2+ accumulation following addition of 10 mm adenosine triphosphate (atp). 45ca2+ uptake was retarded by the presence of regucalcin (1.0-4.0 microm). this retardation was remarkable at 1 min after regucalcin addition, while appreciable retardation was no longer seen at 5 ... | 1989 | 2632051 |
| effect of the calcium-binding protein regucalcin on the ca2+ transport system in rat liver microsomes: the protein stimulates ca2+ release. | the effect of the calcium-binding protein regucalcin on the ca2+ transport system in the liver microsomes from fed rats was investigated. ca2+ transport was assayed by the method of millipore filtration to estimate microsomal 45ca2+ accumulation following addition of 10 mm adenosine triphosphate (atp). 45ca2+ uptake was retarded by the presence of regucalcin (1.0-4.0 microm). this retardation was remarkable at 1 min after regucalcin addition, while appreciable retardation was no longer seen at 5 ... | 1989 | 2632051 |
| the effect of 10 and 20 gy single dose irradiation on the esophageal mucosa of the rabbit. an electron microscopic study. | the mucosa of rabbit esophagus was irradiated with single doses of 10 and 20 gy respectively. specimens were taken for scanning electron microscopy (sem), and light microscopy investigations. examinations were made 1-14 days after 10 gy and 1-17 days after 20 gy. irradiation resulted in edema during the first days. the thickness of the epithelium was at its lowest value around day 5 after the administration of 10 gy, and on days eight to ten after the administration of 20 gy. damage of the epith ... | 1989 | 2633335 |
| [a comparative study of the main components of the nucleolus in different populations of rat trophoblast cells during differentiation. ii. the nucleoli of the trophospongium cells, the glycogen cells and the secondary giant cells]. | a comparative study was performed of the arrangement of different nucleolar components during differentiation of trophoblast cell populations in the junctional zone of placenta (glycogen cells and trophospongium) and in the secondary giant cells. each cell type is characterized by specific interrelation of nucleolar components. some glycogen cells show signs of segregation of nucleolar components: strands of nucleolar components with fibrillar centers (fcs) are displaced to the periphery of the ... | 1989 | 2637541 |
| distribution, oxygenation, and clonogenicity of macrophages in a murine tumor. | transplantable murine tumors, such as the squamous cell carcinoma growing in c3h mice, include a significant proportion of normal cells. the nature of these cells, their locations relative to the blood supply, their oxygenation status, and ability to incorporate 3h-thymidine were examined by sorting cells staining positive or negative with fluorescein isothiocyanate-conjugated goat antimouse igg. of the cells that were recovered from this tumor, 39% +/- 19 (n = 25) were igg+ cells, and this perc ... | 1989 | 2639726 |
| sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy. | the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ... | 1989 | 2643617 |
| sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy. | the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ... | 1989 | 2643617 |
| [comparison of 2 methods of hemagglutination tests for antibodies against strains of yersinia enterocolitica and yersinia pseudotuberculosis]. | the authors compare results of the assessment of antibodies against bacterial strains of yersinia enterocolitica and yersinia pseudotuberculosis. the examinations were made by the haemagglutination technique. in the reaction of the antigen human and ram erythrocytes were used as carriers. the work comprised 462 examinations of patients with an assumed yersinia aetiology of the disease and 200 healthy controls. the values obtained during parallel examinations with antigens of yersinia enterocolit ... | 1989 | 2646030 |
| laboratory diagnosis of human chlamydial infections. | chlamydia trachomatis is a human pathogen that causes ocular disease (trachoma and inclusion conjunctivitis), genital disease (cervicitis, urethritis, salpingitis, and lymphogranuloma venereum), and respiratory disease (infant pneumonitis). respiratory chlamydioses also occur with infection by avian strains of c. psittaci or infection by the newly described twar agent. diagnosis of most acute c. trachomatis infections relies on detection of the infecting agent by cell culture, fluorescent antibo ... | 1989 | 2650858 |
| dependence of inessential late gene expression on early meiotic events in saccharomyces cerevisiae. | spr3 is one of at least nine genes which are expressed in sporulating saccharomyces cerevisiae cells at the time of meiosis i. we show below that strains homozygous for null alleles of spr3 are capable of normal meiosis and the production of viable ascospores. we have also monitored spr3 expression in a series of strains that are defective in meiotic development, using an spr3:lacz fusion carried on a single copy plasmid. beta-galactosidase activity occurred at wild-type levels in diploid strain ... | 1989 | 2651894 |
| relevance of hepatitis b viral dna in assessment of potential liver allograft recipients. | | 1989 | 2652828 |
| bovine abortion associated with yersinia pseudotuberculosis infection. | | 1989 | 2652872 |
| [biological activity of tubercule bacilli in an epizootic infectious process]. | the studies showed that during epizootic infectious processes of tuberculosis in cattle and due to multiple subcultivation and long-term storage on artificial nutrient media tubercle bacilli changed their properties and biological activity which was evident first of all from their lower virulence and sensitizing capacity. | 1989 | 2654923 |
| cerebral metabolic response and mitochondrial activity following insulin-induced hypoglycemia in newborn lambs. | we evaluated the newborn lamb's cerebral cellular activity and metabolism following acute insulin-induced hypoglycemia. eleven animals received an insulin bolus followed by a continuous infusion to maintain a plasma glucose of 1 mm/l for 2 h, while 8 other animals received an equivalent dose of saline. following the induction of hypoglycemia, the animals became quiet and transient seizures were observed in 3 animals. a significant increase in heart rate (p less than 0.01), and a decrease in arte ... | 1989 | 2655716 |
| superior mesenteric vein thrombosis caused by yersinia pseudotuberculosis. | | 1989 | 2655810 |
| asymmetric dna bending induced by the yeast multifunctional factor tuf. | tuf is a yeast regulatory factor that binds to conserved dna sequence elements involved in gene activation or silencing as well as in telomere function. using gel electrophoresis analyses, we show here that tuf induces dna bending at a site located upstream of the recognition sequence (rpg box). several point mutations in the rpg box reduced tuf binding strength without affecting the extent of bending. selective proteolysis of tuf.dna complexes further suggested the existence of two separate pro ... | 1989 | 2656680 |
| evaluation of enzyme immunoassay for the detection of pathogenic yersinia enterocolitica and yersinia pseudotuberculosis strains. | to determine the virulence plasmid-harboring strains of yersinia enterocolitica, we prepared antiserum against plasmid-encoded proteins of y. enterocolitica serotype o3 and carried out an enzyme immunoassay (eia) against temperature-inducible released proteins. this serum reacted with proteins released from not only a y. enterocolitica serotype o3 strain but also y. enterocolitica serotype o5:27, o8, and o9 and y. pseudotuberculosis serotype 1b, 2a, 2b, 2c, 3, 4a, 4b, 5a, 5b, 6, 7, and 8 strains ... | 1989 | 2656747 |
| special features of distribution of yersinia pseudotuberculosis in japan. | the incidence of yersinia pseudotuberculosis isolation from humans, animals, water, and pork in japan is presented on the basis of a review of the literature and serotyping of 633 strains referred to our laboratory. most of the strains belonged to serogroup 4b, followed in frequency of isolation by serogroups 3, 1b, 5b, 5a, and the others, whereas serogroup 1a has not been detected. although strains were isolated from 11 species of animals, none were isolated from birds. | 1989 | 2656752 |
| high degree of conservation between ribosomal proteins of yersinia pseudotuberculosis and escherichia coli. | | 1989 | 2657663 |
| two outbreaks of yersinia pseudotuberculosis 5a infection in japan. | in the summer of 1984 there occurred 2 outbreaks of infection with yersinia pseudotuberculosis 5a. 58 persons ate meat products, vegetables and rice at a japanese barbecue restaurant. 39/58 (67%) developed symptoms. in 19/39 persons an infection with y. pseudotuberculosis could be diagnosed by stool cultures and/or serological techniques. fever occurred in all patients, exanthema in 68% and abdominal pain in 68%. thus, human yersiniosis due to y. pseudotuberculosis 5a could be a febrile disease ... | 1989 | 2658021 |
| lack of correlation between congo red binding and enteroinvasiveness in escherichia coli. | | 1989 | 2659682 |
| cytoskeletal proteins including myofilaments in human tumors. | | 1989 | 2660368 |
| yersinia pseudotuberculosis and arthritis. | in 1986 and 1987 nine patients with a raised antibody titre to yersinia pseudotuberculosis were found. two of these results were almost certainly due to the cross reactivity between some y pseudotuberculosis serogroups and some salmonella serotypes. of the other seven patients with otherwise unexplained y pseudotuberculosis serology, three suffered from severe arthritis and two had symptoms suggestive of sacroiliitis. our data and published reports suggest that reactive arthritis might follow bo ... | 1989 | 2662919 |
| [yersinia infection simulating acute appendicitis]. | | 1989 | 2666715 |
| acute interstitial nephritis associated with yersinia pseudotuberculosis infection. | we report two cases of acute interstitial nephritis associated with yersinia pseudotuberculosis infection. the patients had fever, abdominal pain, vomiting and acute renal failure coinciding with elevated agglutination antibody titer for y. pseudotuberculosis. renal biopsy revealed interstitial nephritis in both patients. although it is well known that yersiniosis sometimes affects glomeruli, this is the first report to demonstrate acute interstitial nephritis in patients with y. pseudotuberculo ... | 1989 | 2667363 |
| a new tool for the serodiagnosis of acute plasmodium falciparum malaria in individuals with primary infection. | we have developed an elisa which detects, with high specificity, antibodies against a major surface protein of p. falciparum merozoites which is a processing product of the precursor glycoprotein gp190. this assay can be used in the diagnosis of acute malaria in individuals with primary infection. two partial sequences of gp190 were expressed in e. coli as beta-galactosidase (beta-gal) fusion proteins. the same sequences fused to chloramphenicol acetyltransferase (cat) or mouse dihydrofolate red ... | 1989 | 2668417 |
| liver disease associated with exposure to 1,1,1-trichloroethane. | 1,1,1-trichloroethane is a halogenated hydrocarbon solvent commonly used in industry because of its supposed lack of hepatotoxicity. nonetheless, animal studies performed by several independent groups have shown the solvent to induce fat deposition, vacuolar degeneration, and centrilobular necrosis, changes similar to those seen after exposure to carbon tetrachloride, albeit of a much reduced magnitude, in animals exposed to the agent. four patients with fatty liver disease whose work entailed s ... | 1989 | 2669664 |
| [clinical aspects, pathogenesis and treatment of yersinia infections]. | | 1989 | 2671487 |
| expression and partial purification of human pro-opiomelanocortin in escherichia coli. | a large portion of the human pro-opiomelanocortin (pomc) peptide corresponding to amino acid residues 59-241 has been cloned and expressed in escherichia coli. a 1.0 kb dna fragment encoding this peptide was cloned into the expression vectors puc8 and pur291. plasmid pjmbg51 (a puc8 recombinant) was found to direct the expression of a 24 kda peptide. the recombinant pur291 (pjmbg52) was shown to produce a beta-galactosidase fusion protein of 140 kda. western blot analysis showed that both the 24 ... | 1989 | 2673285 |
| [the first case of pseudotuberculosis found in china]. | this paper reports the first case of pseudotuberculosis in china. from the patient's stool, a strain of yersinia pseudotuberculosis was isolated. the isolate has all the classical characteristics of yersinia pseudotuberculosis and belongs to serotype iv. | 1989 | 2673528 |
| [conformational stability and immunochemical properties of yersinin--a basic protein of the outer membrane of the pseudotuberculosis microbe]. | using spectroscopic methods (circular dichroism and intrinsic protein fluorescence) and immunoenzyme assay, changes in the spatial and antigenic structure of yersinin, porin from outer membrane of yersinia pseudotuberculosis, were studied in solutions of ionic and non-ionic detergents at various temperatures and low ph values. yersinin was shown to retain its secondary structure under various denaturation conditions, the content of regular structural patterns depending on specific action of the ... | 1989 | 2675850 |
| na+ electrochemical gradient and na+-ca2+ exchange in rat proximal tubule. | the basolateral cell membrane of the rat proximal tubule contains a na+-ca2+ exchanger that may participate in the regulation of cytosolic calcium (cai) and ca2+ transport. in this work, the activity and orientation of the na+-ca2+ exchanger was studied in rat proximal tubules. the experiments were based on the thermodynamic notion that the exchanger is driven by the prevalence of either of two electrochemical gradients, that for na+ (delta mu na+) or for ca2+ (delta mu ca2+). reductions in delt ... | 1989 | 2679143 |
| rapid growth and early rupture of a primary mycotic aneurysm of the abdominal aorta. | a case of a primary mycotic aneurysm of the abdominal aorta due to infection with staphylococcus aureus is presented. the outcome was fatal because of rupture into the jejunum. | 1989 | 2680612 |
| yersinia pseudotuberculosis infection as a cause of reactive arthritis as seen in a genitourinary clinic: case report. | patients are often referred to the genitourinary clinic for screening for urethral infections after a clinical diagnosis of reiter's syndrome or reactive arthritis. we report a case of reactive polyarthritis in which serological evidence of yersinia pseudotuberculosis infection was found in the absence of the other common precipitating organisms, such as chlamydia spp, klebsiella spp, shigella spp, campylobacter spp, or yersinia enterocolitica. | 1989 | 2680895 |
| measurement and clinical significance of preimplantation blastocyst gonadotrophins. | | 1989 | 2681735 |
| [analysis of the plasmid composition of yersinia pseudotuberculosis strains and its use for typing pseudotuberculosis pathogens]. | electrophoresis in agarose gel has been used to study the plasmid spectra of 854 yersinia pseudotuberculosis strains isolated from different sources. the plasmids found in the microbial strains are represented by the elements with molecular masses 82; 57; 45; 5.5; 4.4; 3.5; 2.7; 2.4; 2.3 md. the variable spectra of plasmids is peculiar only for serovar i of yersinia pseudotuberculosis. plasmids p45 and p82 are classified as the main, while other plasmids as auxiliary ones. in accord with the cla ... | 1989 | 2682219 |
| a comparison of structural features of the walls of coronary arteries from 10 different species. | the intimal thickness of the coronary arteries of humans less than 20 years of age was compared with that 9 other species. it was found that humans were unique amongst the species studied in showing substantial intimal thickening at an early age. the intimal thickening was associated with defects of the internal elastic lamina (iel), which in humans were not repaired, but which in other species were associated with an effective reduplication of the iel. this reformed iel appeared in other specie ... | 1989 | 2682490 |
| haemochromatosis and aldosterone deficiency presenting with yersinia pseudotuberculosis septicaemia. | a 50 year old man presented with a pyrexial illness following a holiday abroad. yersinia pseudotuberculosis was isolated from blood culture. response to appropriate antibiotic therapy was prompt and complete, but full recovery was complicated by an episode of hyperkalaemia, hyponatraemia and the passing of large volumes of dilute urine. three years previously he had developed diabetes mellitus and hypogonadotrophic hypogonadism. investigation on recovery showed underlying haemochromatosis and al ... | 1989 | 2682586 |
| identification and characterization of a nuclear localization sequence-binding protein in yeast. | nuclear proteins contain specific regions that are required for entry into the nucleus. using ligand blotting, we have shown that a 67-kda yeast nuclear envelope protein (p67) recognizes synthetic peptides containing the yeast histone h2b or simian virus 40 large tumor antigen nuclear localization sequence. both free peptide and peptide conjugated to human serum albumin are recognized. the interaction between p67 and the nuclear localization sequences is specific; neither a mutant peptide that i ... | 1989 | 2682660 |
| functional organization of the extrinsic and intrinsic circuitry of the parahippocampal region. | | 1989 | 2682783 |
| [the differential diagnosis of salmonella abortus ovis and yersinia pseudotuberculosis in sheep abortions]. | 55 strains of salmonella abortus ovis and 3 strains of yersinia pseudotuberculosis (two serotypes i, one serotype ii), isolated out of aborted ovine fetuses from northern bavaria, were investigated and compared culturally, biochemically and serologically. the identification of s. abortus ovis (group b) could not be achieved by use of api 20 e. the code-number 4004500/47 identified s. typhi (group d) only. y. pseudotuberculosis serotype ii but not serotype i agglutinated with the polyvalent and t ... | 1989 | 2683516 |
| [the plasmid composition and pathogenetic characteristics of yersinia pseudotuberculosis strains isolated from human subjects against a background of epidemic and sporadic morbidity]. | 112 newly isolated clinical cultures of y. pseudotuberculosis have been studied. the strains have been characterized by the presence of plasmids and pathogenicity signs associated with plasmids. the results of the study have confirmed the decisive role of the plasmid with a molecular weight of 44-48 md in the virulence of y. pseudotuberculosis. the plasmid with a molecular weight of 82 md, previously attributed the role of an epidemic marker, has also been found to be widely spread. our study ha ... | 1989 | 2683522 |
| [findings of yersiniosis in the crimea]. | | 1989 | 2683523 |
| a method for sampling the stages of amelogenesis on mandibular rat incisors using the molars as a reference for dissection. | a method for locating specific stages of amelogenesis on continuously erupting incisors was devised for rats weighing 101 +/- 5 g (n = 32). the technique is based on reflecting reference lines from the mandibular molars as perpendiculars to the labial surface of mandibular incisors. from these reference lines additional measurements are then made along the midline of the labial surface of the incisor in an apical or incisal direction to find the site desired for sampling. histological studies on ... | 1989 | 2683870 |
| [outbreaks of pseudotuberculosis and intestinal yersiniosis among soviet specialists and members of their families in the mongolian people's republic]. | in summer and winter of 1986 two outbreaks of alimentary enteric diseases occurred among soviet specialists and members of their families in ulan bator. these diseases were identified as yersinia infections registered in mongolia for the first time. in july 114 children in a kindergarten fell ill after being fed with salad prepared from vegetables and spring onions supplied from a nearby state farm. 20 y. pseudotuberculosis cultures, serovar 1, were isolated, and in 25 persons antibodies to the ... | 1989 | 2686302 |
| regulator and enzyme specificities of the tol plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. | the tol plasmid upper pathway operon encodes enzymes involved in the catabolism of aromatic hydrocarbons such as toluene and xylenes. the regulator of the gene pathway, the xylr protein, exhibits a very broad effector specificity, being able to recognize as effectors not only pathway substrates but also a wide variety of mono- and disubstituted methyl-, ethyl-, and chlorotoluenes, benzyl alcohols, and p-chlorobenzaldehyde. benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase, two upper pa ... | 1989 | 2687253 |
| cat-contaminated environmental substances lead to yersinia pseudotuberculosis infection in children. | a 1-year-old boy was infected with yersinia pseudotuberculosis serotypes 1b and 3, and his 3-year-old brother was infected with y. pseudotuberculosis serotype 1b; both had drunk water from puddles in a garden of their housing district of miyoshi city, hiroshima prefecture, japan. the y. pseudotuberculosis serotype 1b and 3 strains isolated from soil from the dried-up puddles and sand and feces from the sandbox proved to be from a stray cat. the restriction endonuclease patterns of the plasmid in ... | 1989 | 2687319 |
| [clinical aspects of yersinia pseudotuberculosis infections]. | during 2 years 27 cases of yersinia pseudotuberculosis infections were diagnosed in the infectious ward. on the basis of literary data and her own cases the author discusses in details the clinical aspects and varying forms of yersinia pseudotuberculosis infections. the attention is drawn to the fact that the pathogen is widespread, to the incidence of human infections and to the difficulties of differential diagnostics. | 1989 | 2689952 |
| yersinia pseudotuberculosis enteritis in four calves. | a yersinia pseudotuberculosis enteritis in four yearling calves is described. this is the first report of this organism causing enteric disease in domestic cattle in north america. | 1989 | 2691537 |
| mechanisms involved in the pathogenesis of yersinia infections. | yersinia enterocolitica and yersinia pseudotuberculosis are food-borne enterobacterial pathogens which may initiate rheumatoid diseases. by molecular genetic analysis of the pathogenicity of these species virulence gene loci could be identified on the chromosome and on a plasmid. plasmid-encoded proteins mediate cell adherence, phagocytosis resistance, survival in serum, cytotoxicity, and collagen binding. y. enterocolitica of serotype 0:8 is mouse-lethal and arthritogenic for lewis rats. mouse ... | 1989 | 2692128 |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| [various approaches to studying molecular-genetic mechanisms of low-temperature adaptation of microbial populations]. | the effect of biologically low temperature (12 degrees c) on the parameters of microbial population such as survival, catalase activity and its isoenzyme spectrum have been investigated on the models of escherichia coli and yersinia pseudotuberculosis. the quantity of nucleic acids, plasmid spectrum and temperature effect on the level of plasmid dna spiralization were studied under these conditions. the process of molecular genetic adaptation of bacterial populations having the broad temperature ... | 1989 | 2693957 |
| isolation and properties of carboxylesterase p4 from yersinia pseudotuberculosis. | the carboxylesterase p4 produced by yersinia pseudotuberculosis was purified 330-fold by gel permeation and deae-trisacryl chromatography with a final yield of 21%. the apparent molecular weight, as determined by fast-protein liquid chromatography, was 45 kda. the hydrolytic activity of esterase p4 was higher with the 1-naphthyl esters than with the 2-naphthyl esters of acetic, propionic and butyric acids. the apparent km values were identical for 1-naphthyl acetate and 1-naphthyl propionate (0. ... | 1989 | 2694247 |
| ion exchange paper adsorption and thin-layer chromatography for detection of chloroquine in urine for the study of chemosensitivity of plasmodium falciparum. | with a view to determining the chemosensitivity of plasmodium falciparum to chloroquine, two qualitative tests for the detection of this antimalarial agent in urine were compared in central african schoolchildren: the test of dill and glazko and a thin-layer chromatography technique involving ion exchange and extraction. results obtained with 149 subjects showed the marked superiority in specificity and sensitivity of the chromatography test. this is of particular interest when selecting subject ... | 1989 | 2694983 |
| [the diagnostic value of immunoenzyme analysis in pseudotuberculosis]. | | 1989 | 2698536 |
| [dynamic clinico-laboratory indices of physical and mental work capacity in pseudotuberculosis]. | | 1989 | 2698537 |
| risk of carcinogenesis from exposure to dietary mutagens. | epidemiological studies revealed the crucial role of dietary habits in human cancer. this review will summarize literature data on naturally occurring mutagens and carcinogens. the risk of carcinogenesis related to dietary mutagens will be discussed. | 1989 | 2698602 |
| proteins of the inner and outer centromere of mitotic chromosomes. | we have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. these studies permit us to resolve two distinct regions: the inner and outer centromere. (i) components of the outer centromere: autoantibodies from certain patients with rheumatic disease identify a family of three immunologically related polypeptides that we have designated cenp-a (17 kda), cenp-b (80 kda), and cenp-c (140 kda). cenp-b has been cloned and seque ... | 1989 | 2698830 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| peroxisomal palmityl coa oxidase activity in ocular tissues and cultured ciliary epithelial cells. | the activity of palmityl coa oxidase, a peroxisomal enzyme, was determined in bovine ocular tissues. significant levels of activity were found in the iris, ciliary body and pigmented epithelium-choroid but little or no activity was detected in the corneal epithelium, lens capsule-epithelium and retina. treatment of bovine ciliary epithelial cells with clofibric acid for 72 hours in culture resulted in a several fold enhancement of palmityl coa oxidase activity. these results suggest that peroxis ... | 1989 | 2715677 |
| family study of common fragile sites. | the frequency of folate-sensitive common fragile sites (1p31, 1q44, 3p14, 3q26.2, 6q26, 16q23, xp22.3) was determined in 19 healthy individuals from four families. the individuals consisted of 12 males and 7 females from 1 to 59 years of age. the frequency showed intrafamilial variation, but we were unable to demonstrate that the frequency was inherited in a mendelian codominant fashion. in eight subjects whose chromosome 3 homologues could be distinguished by q-band polymorphism, breakages at 3 ... | 1989 | 2722197 |
| family study of common fragile sites. | the frequency of folate-sensitive common fragile sites (1p31, 1q44, 3p14, 3q26.2, 6q26, 16q23, xp22.3) was determined in 19 healthy individuals from four families. the individuals consisted of 12 males and 7 females from 1 to 59 years of age. the frequency showed intrafamilial variation, but we were unable to demonstrate that the frequency was inherited in a mendelian codominant fashion. in eight subjects whose chromosome 3 homologues could be distinguished by q-band polymorphism, breakages at 3 ... | 1989 | 2722197 |
| effect of cocaine and lidocaine on the development of kindled seizures. | the effect of a subconvulsive dose of cocaine or lidocaine on the development of kindling was studied in male long-evans rats. animals were divided into three groups and kindled by daily electrical stimulation of the pyriform cortex. fifteen minutes before each stimulation each animal received an intraperitoneal injection of either saline, 20 mg/kg cocaine hydrochloride, or 20 mg/kg lidocaine hydrochloride. following kindling the drug treatment was discontinued and the transfer of kindling to a ... | 1989 | 2727005 |
| sarcolemmal integrity during ischaemia and reperfusion of the isolated rat heart. | the effects of ischaemia and reperfusion on sarcolemmal permeability were studied in the isolated rat heart using an ionic lanthanum probe technique. detection of increased permeability after exposure to short periods of ischaemia without accompanying reperfusion effects was achieved by employing a substrate-free, low calcium saline solution as vehicle for ionic lanthanum. quantifiable results showed a rapid increase in sarcolemmal permeability in the subendocardium after 15 min of ischaemia, wi ... | 1989 | 2730527 |
| electron-microscopic demonstration of olfactory-marker protein with protein g-gold in freeze-substituted, lowicryl k11m-embedded rat olfactory-receptor cells. | in this study electron-microscopic immunocytochemistry was used to localize olfactory marker protein in olfactory epithelia. rat olfactory-epithelial samples were rapidly frozen, freeze-substituted with acetone, embedded at low temperatures with lowicryl k11m and labelled on the sections with polyclonal antibodies raised against olfactory marker protein and with protein g conjugated to colloidal gold. apart from the aforementioned use of acetone, substitution was carried out in the complete abse ... | 1989 | 2731216 |