| long-term antibody synthesis in vitro- iv. independent segregation of antibodies directed to different determinants of an antigen molecule in its native configuration. | independent segregation of antibody populations directed to different portions of e. coli beta-d-galactosidase occurs during the immune response against the enzyme. anti-enzyme antibodies able to interact and activate a naturally occurring ligand, the mutant-defective enzyme amef (antibody mediated enzyme factor), do not parallel anti-enzyme antibodies which are measured by a coprecipitation assay involving precipitation of the wild-type molecule. dissociation of the two antibody populations is ... | 1976 | 61938 |
| evidence for a role of n-acetylmuramyl-l-alanine amidase in septum separation in escherichia coli. | septum formation and septum separation have been studied in a chain-forming mutant of escherichia coli k-12 bearing the enva mutation and its parental strain. in comparison to the wild type, the mutant showed a sixfold reduction in the specific activity of the enzyme, n-acetylmuramyl-l-alanine amidase (ec 3.5.1.28), part of which was associated to the outer membrane. genetic as well as physiological suppression of chain formation resulted in an increase in amidase activity. the addition of n-ace ... | 1976 | 61961 |
| stepwise biosynthesis in vitro of globin genes from globin mrna by dna polymerase of avian myeloblastosis virus. | two approaches have been explored for the synthesis of double-stranded dna from single-stranded dna template complementary to rabbit 9s globin mrna (cdna). (i) cdna was elongated with dcmp or dtmp homopolymeric tracts using terminal deoxynucleotidyltransferase (ec 2.7.7.31; nucleosidetriphosphate:dna deoxynucleotidylexotransferase). cdna-dc, in the presence of an oligo(dg)10 primer, was an efficient template with either dna polymerase of escherichia coli (ec 2.7.7.7; deoxynucleosidetriphosphate: ... | 1976 | 62360 |
| [arthritis related to intestinal anastomoses]. | jejuno-colic and jejuno-ileal anastomoses may provoke arthritis. the recently recognized physiopathology is that of arthritis due to immune complexes related to pullulation of escherichia coli and of bacilus fragilis. these cases of arthritis, usually sensitive to therapy, have, in some stutborn cases, required the re-establishment of intestinal continuity, which in each case has made the joint phenomena disappear. | 1976 | 62379 |
| antigenic degradation in escherichia coli. | strains of escherichia coli isolated in a maternity unit were examined serologically. on primary isolation the strains were shown to be o-antigenically distinguishable, although having the same h antigen, biotype and antibiogram. subsequent detailed serological studies revealed that the colonial variants derived from them showed similar antigenic diversity, irrespective of the original antigenic structure. | 1976 | 62472 |
| a gram stain for smears of blood cultures, body fluids and tissues. | a new gram stain method for blood cultures was adapted from a method for tissue sections. this was accomplished by comparing various modifications of the latter method on positive smears prepared from a 24-hour negative blood culture seeded with staphylococcus aureus and escherichia coli. a blind study was then conducted using the new method and hucker's method on 24-hour and 72-hour blood cultures as well as a limited number of other body fluids and tissues. of the 35 positive blood cultures de ... | 1976 | 62513 |
| the agglutinating antibody response in the duodenum of infants with enteropathogenic escherichia coli gastroenteritis. | the agglutinating antibody response in duodenal fluid and serum was measured serially in 15 infants with enteropathogenic escherichia coli gastroenteritis. peak levels of duodenal agglutinins were recorded 8-18 days after the onset of symptoms, and the titres fell within the next 7-14 days. the immunoglobulin (ig) class of these antibodies was mainly iga, but igm antibodies were detected early in the response, especially in the younger infants. late antibodies showed less cross-reactions with ot ... | 1976 | 62645 |
| studies on the pathogenesis of enteric infections caused by invasive bacteria. | salmonellae, shigellae and some escherichia coli must invade the intestinal epithelial cell and multiply within the mucosa to cause disease. although the bacterial cell most likely possesses several properties essential to this invasive ability, the nature of the cell envelope complex is at present the only characteristic which has been implicated in this process. while a number of pathophysiological events result from invasion, some of our recent efforts have concerned the site and mechanism of ... | 1976 | 62646 |
| role of ionic strength in salt antagonism of aminoglycoside action on escherichia coli and pseudomonas aeruginosa. | studies were designed to determine whether ionic strength (mu) is a significant factor in salt inhibition of aminoglycoside action against escherichia coli and pseudomonas aeruginosa. in both nutrient broth (a low mu medium) and mueller-hinton broth (a relatively high mu medium), protection of e. coli from dihydrostreptomycin or gentamicin action by mgcl2, nacl, or na2so4 was attributed to ionic strength alone. the percentage of protection increased with ionic strength and was independent of the ... | 1976 | 62813 |
| five new escherichia coli k antigens, k95, k96, k97, k98 and k100. | five escherichia coli strains were established as antigenic test strains for five new polysaccharide k antigens: k95, k96, k97, k98 and k100. k95 to k98 served already as test strains of o antigens o75, o77, o81 and o107 respectively. f147, which is test strain of k100, had o antigen o75. | 1976 | 63221 |
| inhibition of the mitogenicity of the carrier molecule results in loss of immunogenicity of a hapten-lps conjugate. | colistin methanesulfonate, a basic polypeptide similar to polymyxin e, has been shown to suppress the mitogenicity of lipopolysaccharide (lps) from e. coli. it also inhibits the immunogenicity of a hapten-lps conjugate. the inhibition was neither due to interference with the expression of hapten determinants, nor was it due to crossreactivity between the hapten and colistin methanesulfonate. as mitogenicity and immunogenicity was similarly affected, we conclude that activation of bursa-derived l ... | 1976 | 63227 |
| [successes and prospects for genetic engineering]. | the review of literature (1970-1976) on problems of gene engineering is given. gene engineering is pointed out to be a new method of modern biology and a new page of modern molecular genetics. gene engineering detected a real possibility of artificial creating living hybrid organisms, i.e. constructing functional recombinant dna molecules according to a project of investigator, but not to possibilities of crossing. the determination of gene engineering (in contrast with genetical engineering) is ... | 1976 | 63407 |
| enterotoxigenic escherichia coli causing infantile enteritis in britain. | | 1977 | 63727 |
| mechanism of bleomycin sensitivity on escherichia coli and its possible implication on cancer chemotherapy. | | 1976 | 63971 |
| on the behaviour of arginyl-trna synthetase of escherichia coli in the presence of arginine precursors. | | 1976 | 64171 |
| partial purification of the argecbh bipolar operon of escherichia coli and of its control region by the use of ecori and hindiii restriction endonucleases. | | 1976 | 64172 |
| amplification of the escherichia coli k 12 arginine bipolar operon: construction in vitro and cloning of a composite cole1-argecbh plasmid. | | 1976 | 64174 |
| comparative analysis of the double-stranded rna sequence synthesized in vitro on rat liver chromatin by rat liver (form b) or escherichia coli rna polymerase. | | 1976 | 64210 |
| determination of the complete amino acid sequence of protein s16 from escherichia coli ribosomes. | | 1976 | 64224 |
| terminal riboadenylate transferase from escherichia coli. characterization and application. | catalytic properties of terminal riboadenylate transferase from escherichia coli and the products of the enzymic reaction were investigated. the kinetic analysis revealed that the reaction obeys the sequential ordered bi-bi mechanism. the application of conditions elaborated in this study resulted in the synthesis of products of defined size and efficient primer utilization. the trna(ra)n obtained was a good template for the synthesis of complementary dna with reverse transcriptase. | 1976 | 64360 |
| influence of serum-derived chemotactic factors and bacterial products on human neutrophil chemotaxis. | the chemotaxis of neutrophils has been shown to be modulated by serum factors, tissue factors, bacterial products, and a host of other substances. in vivo, these factors may act in concert with each other to modify neutrophil movement. we examined the effect of aggregated gamma globulin-activated serum (as), bacterial factors, and endotoxin either alone or in combination with each other, on human neutrophil chemotaxis. exposure of neutrophils to as resulted in deactivation to as but not to esche ... | 1977 | 64455 |
| purification and properties of rauscher leukemia virus dna polymerase and selective inhibition of mammalian viral reverse transcriptase by inorganic phosphate. | rauscher leukemia virus rna-directed dna polymerase has been purified to near homogeneity (greater than 90% pure) using affinity chromatography on polycytidylate-agarose with over 85% recovery of input enzymatic activity. the purified enzyme has a molecular weight of approximately 70,000 and appears to consist of a single polypeptide chain. the enzyme is free of dnase, but has rnase h activity. analysis of the requirements for optimal rates of dna synthesis by this enzyme using synthetic and nat ... | 1977 | 64468 |
| cross-antigenicity and immunogenicity between capsular polysaccharides of group c neisseria meningitidis and of escherichia coli k92. | antibodies to capsular polysaccharides of group c neisseria meningitidis are often found in sera of young adults despite infrequent nasopharyngeal carriage and low rate of attack of n. meningitidis in the united states. thus, experiments were designed for detection of bacteria cross-reactive with n. meningitidis. among 3,264 cultures of stool, urine, blood, and cerebrospinal fluid, only 14 strains were found to be cross-reactive; all were escherichia coli possessing the k92 capsular polysacchari ... | 1977 | 64575 |
| effects of streptovaricins and their degradation products on rna-directed dna polymerase of rauscher leukemia virus. | the activities of streptovaricin complexes, streptovaricins, streptovals, and streptovarinic degradation products were elevated against rna-directed dna polymerases of rauscher leukemia virus, dna-dependent dna polymerase of bacterial and mammalian cells, and dna-dependent rna polymerases of mammalian origin. the activities of streptovaricins were also listed for comparison purposes. the effects of streptovaricin complexes on viral dna polymerases varied significantly from lot to lot, and strept ... | 1977 | 64615 |
| gene specific priming of complementary dna synthesis. | dna, complementary to chicken globin mrna was synthesized using either avian myeloblastosis virus reverse transcriptase, or e. coli dna polymerase i. transcriptase cdna sediments at 9 s on sucrose gradients, and is 620 nucleotides in length, representing a complete copy of globin mrna template. in contrast, polymerase i cdna sediments at 4 s, is 100 to 200 nucleotides in length, and is a copy of a small region at the 3'(poly a) end of globin mrna. similarly, transcriptase cdna and polymerase i c ... | 1976 | 64922 |
| structure and properties of a hybrid tryptophan synthetase of alpha chain produced by genetic exchange between escherichia coli and salmonella typhimurium. | genetic exchange between the structural genes for the alpha chain of tryptophan synthetase [tryptophan synthase; l-serine hydro-lyase (adding indoleglycerol-phosphate), ec 4.2.1.20] of e. coli and s. typhimurium yielded recombinant genes that specified functional hybrid polypeptides. the alpha chains produced by three recombinants appeared to be identical but differed from those of e. coli and s. typhimurium by at least 27 and 8 amino acid residues, respectively. in vivo and in vitro tests of en ... | 1977 | 64983 |
| atp-dependent proton translocation and quenching of 9-aminoacridine fluorescence in inside-out membrane vesicles of a cytochrome-deficient mutant of escherichia coli,. | 1. atp-dependent proton translocation and atp-dependent quenching of the fluorescence of 9-aminoacridine were measured in inside-out vesicles derived from a cytochrome-deficient mutant of escherichia coli. 2. atp-dependent quenching of fluorescence was inhibited by nigericin gramicidin, nh4cl, and carbonylcyanide-m-chlorophenylhydrazone. inhibition was also produced by the atpase inhibitors n,n'-dicyclohexylcarbodimide (dccd) and diphenyl phosphorazidate (dpa), and by the respiratory chain inhib ... | 1977 | 65180 |
| ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections. | a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ... | 1977 | 65417 |
| radioimmunoassay procedure for quantitating bacterial antibody in human sera. | a ria system was developed to detect antibodies in human sera against bacteria. sonicates of escherichia coli and fusobacterium polymorphum were used as antigens to sensitize plastic-coated beads; antibodies to these antigens were detected with 125i-labeled antihuman globulin. serum antibody titers against e. coli were determined by the serial dilution method; from the results the standard curve principle was applied in determining the relative amounts of antibodies against e. coli in serum samp ... | 1977 | 65436 |
| evaluation of methods for the determination of q and k antigens of an 02:k1(l) strain of escherichia coli. | tests made on ten colonies from a strain of escherichia coli o2:k1 demonstrated that bacterial agglutination tests were reliable for identifying the o antigen of serogroup o2 but were unreliable for identifying the k1 antigen. the granular nature of k agglutination was not a reliable characteristic of the l type of k antigen. in contrast, indirect haemagglutination, immunodiffusion and immunoelectrophoresis tests with bacterial extracts gave consistent results with all colonies. the polysacchari ... | 1977 | 65474 |
| escherichia coli meningitis and congenital tuberculosis in the same infant. | | 1977 | 65681 |
| k antigen determination of escherichia coli by counter-current immunoelectrophoresis (cie). | the application of the counter-current immunoelectrophoresis (cie) for determination of e. coli acidic polysaccharide k antigen is described. the most appropriate dilutions of antigens and antisera were established after examination of six different k antigens with homologous ok antisera. according to this result all test strains for acidic polysaccharide k antigens, i.e. k1 to k57, k62, k74, k82, k53, k84, k92, k93, k94, k95, k96, k97, k98 and k100, were examined and ok antisera suitable for th ... | 1977 | 65900 |
| fatty acid synthesis in escherichia coli is indirectly inhibited by phenethyl alcohol. | experiments were performed to determine how phenethyl alcohol inhibits phospholipid synthesis in e. coli. at a nonbacteriostatic concentration, the drug reduces the rate of de novo fatty acid and phospholipid synthesis by 60 to 70%. the inhibition of fatty acid synthesis was found to be a secondary consequence of the inhibition of phospholipid synthesis. phenethyl alcohol reduces the rate of incorporation of exogenous fatty acids into the phospholipids of a fatty acid auxotroph by 60%. these res ... | 1977 | 66062 |
| [isolation of e coli with the aid of indicator paper]. | | 1976 | 66382 |
| does a bacterial elongation factor share a common evolutionary ancestor with actin? | protein synthesis elongation factor tu from e. coli shares several physical, chemical, and functional properties with actin-like proteins. limited tryptic degradation indicates that the two polypeptides have a similar molecular architecture. these observations suggest that they could have evolved from a common ancestor, although more information will be necessary to prove or disprove this hypothesis. a partial sequence, comprising 22 aminoacid residues from the aminoterminal end of the large try ... | 1976 | 66419 |
| frequency of e. coli k antigens in urinary-tract infections in children. | the somatic (o) and casular (k) antigens of escherichia coli from the urine of patients with acute pyelonephritis, acute cystitis, and asymptomatic bacteriuria, and in the faeces of healthy schoolchildren have been investigated. typing antisera for sixteen capsular acidic polysaccharide k antigens were used, and five (numbers 1, 2, 3, 12, and 13) accounted for 70% of isolates from patients with acute pyelonephritis. these five k antigens were found to a lesser extent in the three other study gro ... | 1977 | 66470 |
| inhibition of the lethality of bleomycin a5 in l-cells by hirudonine. | the lethality of several individual bleomycin derivatives, i.e., the spermidine derivative (a5), the dimethyl sulfonium aminopropyl derivative (a2), and the agmatine derivative (b2), was compared on mouse fibroblasts. the spermidine derivative, bleomycin a5, was the most toxic, producing more than a 2-log drop in clonability in 50 hr at 20 microng/ml. 6-azauridine, a relatively nonlethal inhibitor of rna synthesis and cell multiplication, produced a 60% decrease of adenine incorporation into nuc ... | 1977 | 66981 |
| inhibition of the reverse transcriptase of bovine leukemia virus by antibody in sera from leukemic cattle and immunological characterization of the enzyme. | sera from some leukemic cattle contain an antibody that inhibits the reverse transcriptase activity of the bovine leukemia virus. the antibody is not directed against the synthetic template or the major internal and envelope viral antigens. the antibody failed to inhibit the dna polymerases of the murine leukemia virus, simian sarcoma-associated virus, avian myeloblastosis virus, or escherichia coli. conversely, the bovine leukemia virus enzyme was not inhibited by antibody against the reverse t ... | 1977 | 66983 |
| [rna-dependent dna-polymerase of escherichia coli. separation of the enzyme from dna-polymerase i, purification and properties]. | | 1977 | 67016 |
| an investigation of mistranslation in vivo induced by streptomycin by an examination of the susceptibility of abnormal proteins to degradation. | proteolysis rates in vivo were measured in escherichia coli cultures during treatment with dihydrostreptomycin and under various other conditions. dihydrostreptomycin treatment caused an increase in the proteolysis rate, compared to untreated controls. the proteolytic system in vivo responsible for the elevated proteolysis in the early stages of dihydrostreptomycin treatment, or that during canavanine and puromycin treatment, were not inhibited by addition of phenylmethanesulphonyl fluoride. thi ... | 1977 | 67037 |
| regulation of ribonucleoside diphosphate reductase synthesis in escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis. | inhibition of deoxyribonucleic acid (dna) synthesis in escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnae and dnab) or initiation (dnaa) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, which catalyzes the first reaction unique to the pathway leading to dna replication. in contrast to the gudas and pardee proposed model for control of the synthesis of dna repair enzyme ... | 1977 | 67110 |
| counterimmunoelectrophoresis in the diagnosis of bacterial meningitis. | the aim of the present study was to investigate whether counterimmunoelectrophoresis (cie) would facilitate the rapid, etiological diagnosis of bacterial meningitis when used in parallel with other routine methods in a medical bacteriological laboratory. of 3,674 consecutive specimens of cerebrospinal fluid (csf) received at the department of diagnostic bacteriology, statens seruminstitu, 283 specimens (each representing one patient) were selected for examination by cie on the basis of the follo ... | 1977 | 67124 |
| modulation of lipopolysaccharide (lps)-mediated function by structural differences of two physically distinct fractions of escherichia coli k235 lps. | lipopolysaccharide (lps), extracted from escherichia coli k235 by the butanol water technique, was fractionated by gel filtration chromatography into high m.w. (lps i) and low m.w. (lps ii) fractions. these two forms of lps were characterized by different densities and chemical compositions. chemical analysis provided evidence for greater amounts of lipid a and lipd a-associated protein (lap) per unit weight associated with lps ii. the biologic activity of the two lps preparations was compared o ... | 1977 | 67150 |
| new procedure for the direct analysis of in vitro reverse transcription of rous sarcoma virus rna. | based on the observation that in vitro transcription of rous sarcoma virus (rsv) rna by avian myeloblastosis virus dna polymerase renders the rna progressively more sensitive to escherichia coli rnase h digestion, a new procedure for the in situ analysis of this process has been developed. in vitro transcription products of 32p-labeled rsv rna are first treated with rnase h, the resistant fraction is then digested to completion with rnase t1, and the oligonucleotides are analyzed by a fingerprin ... | 1977 | 67218 |
| studies on a case of suppurative thyroiditis. | a patient with suppurative thyroiditis due to infection with escherichia coli is described. serial studies of thyroid function were performed. before the abscess was drained there was no uptake of radio-iodine by the thyroid and there was some spillage of iodinated compounds from the gland. post-operatively circulating thyroid hormone concentrations declined but were restored at four weeks. thyroid uptake of radio-iodine was re-established two weeks after operation but there was little secretion ... | 1977 | 67736 |
| on the combined action of drugs on the lambda prophage induction in escherichia coli k12 cells. | the capability of methotrexate, jododeoxyuridine and 5-fluorouracil to induce lambda prophage was compared when given alone or in combination. all these drugs were found to cause inducing conditions in escherichia coli k12(lambda) cells. combined action of jododeoxyuridine and methotrexate resulted in a pronounced increase in the number of free phages compared with that resulting the treatment either with methotrexate of jododeoxyuridine alone. treatment with 5-fluorouracil caused inactivation o ... | 1977 | 67750 |
| mechanism of conjugation and recombination in bacteria. xvii. further evidence for single-stranded regions in recipient dna during conjugation in escherichia coli k-12. | the secondary structure of recipient dna mated with hfr strain was investigated by cscl density gradient fractionation. after 45 min of hfrh64 x 3h-f-ab1157 mating one-fourth of the radioactive recipient dna was recovered as a single-strand but only after shearing of cell lysates prior to centrifugation. this heavier than native dna fraction of radioactive material (obtained after the first centrifugation) was degraded by single-strand specific nuclease s from aspergillus oryzae. these findings ... | 1977 | 67752 |
| transfer of r1drd19 plasmid from escherichia coli j53 to rhizobium trifolii by conjugation. | rldrd19 plasmid was transferred by conjugation from escherichia coli j53 to rhizobium trifolii t24, t26 and 24xsm strains with frequency 10(-3) to 10(-5). the r. trifolii exconjugants carrying rldrd19 were in turn able to transfer the r factor by conjugation to other r. trifolii strains. rldrd19 was maintained stably in r. trifolii. r. trifolii 24xsm, t26 and 14m were also found to harbor an endogenous plasmid (molecular weight 5.5 megadaltons) with undetermined as yet properties. ridrd19 could ... | 1977 | 67758 |
| kinetics of pair formation during cell cycle of hfr and f in escherichia coli k-12. | pair formation ability in mating mixture of synchronized hfr or f- culture and asynchronous culture of appropriate partner was studied. the obtained results allow to conclude that the pair formation ability of f- cells is stable over the whole cell cycle whereas in hfr cells is it subject to cyclic changes with maximum in the first half of the cycle. | 1977 | 67760 |
| dependence of efficiency of dna transfer and recombinat formation on cell cycle of hfr in escherichia coli k-12. | the efficiency of dna transfer during the hfr cell cycle, studied with the use of 3h-thymidine, is the highest in the first half of the cycle. the efficiency of recombinant formation in the hfr cell cycle demonstrates a similar periodicity only when the ratio of 1 hfr cell to 8 or more f- cells in mating mixture is maintained. the absence of such changes in the number of recombinants during the cell cycle of a donor with relative excess of hfr cells seems to be caused by limitation of the number ... | 1977 | 67761 |
| recipient ability of female and male (hfr) cells of escherichia coli during their cell division cycle. | | 1977 | 67771 |
| superinfection immunity in rec- cells of escherichia coli. | | 1977 | 67772 |
| antigen degradation in human colon ecosystems. host's abo blood type influences enteric bacterial degradation of a cell surface antigen on escherichia coli o86. | | 1977 | 67982 |
| rabbit globin mrna: analysis of t1 rnase digestion fragments. | rabbit globin complementary dna made with rna-dependent dna polymerase (reverse transcriptase) was used as a template for in vitro synthesis of 32p-labeled rna and deoxysubstituted rna. the sequences of the nucleotides in most of the fragments resulting from combined ribonuclease t1 and alkaline phosphatase digestion have been determined. in addition, the 3' nearest neighbor was determined for several fragments resulting from digestion with t1 ribonuclease. the utility of the deoxysubstitution t ... | 1977 | 68035 |
| [nutrient medium for obtaining alpha hemolysin from escherichia coli]. | | 1977 | 68135 |
| plasmid mediating mucosal adherence in human enteropathogenic escherichia coli. | | 1977 | 68245 |
| accessibility of proteins in 50s ribosomal subunits of escherichia coli to antibodies: an ultracentrifugation study. | the accessibility of each of the proteins on the 50s ribosomal subunit of escherichia coli was investigated by establishing whether immunoglobulins (igg), specific for each of the 34 proteins from the 50s subunit, were able to bind to the 50s subunit. the main criterion for accessibility was the formation of specific antibody-50s subunit complexes that could be detected by means of analytical ultracentrifugation. the proteins fell into two main groups. immunoglobulins against proteins l1, l2, l3 ... | 1977 | 68433 |
| dna-polymerase inhibitors. rifamycin derivatives. | ten new derivatives of the antibiotic rifamycin with variable side chains at position 3 were synthesized. the inhibitory activity of these derivatives against dna-polymerases isolated from avian myeloblastosis virus, e. coli and calf thymus were studied at various conditions. 3-(2,4,6-trinitrophenylhydrazone-(methyl) rifamycin sv is a strong inhibitor for all the polymerases tested and belongs to the c class inhibitors of reverse transcriptase. 3-(monoallylhydrazone-(methyl) rifamycin sv possess ... | 1977 | 68462 |
| introduction of antigenic determining 2,4-dinitrophenyl residues into 4-thiouridine, n3-(3-l-amino-3-carboxypropyl) uridine and trna-phe from e. coli. | the introduction of antigenic determining 2,4-dinitrophenyl residues into the rare ribonucleosides 4-thiouridine (1a), and n3-(3-l-amino-3-carboxypropyl) uridine (2) as well as into trna-phe from e. coli has been investigated. alkylation of 1a with omega-bromo-2,4-dinitroacetophenone (3b) gives s-(2,4-dinitrophenacyl)-4-thiouridine (5a). applying the reaction to the 5'-monophosphate of 1a, 5b is formed, but this product decomposes at ph 7. however, acylation of 2 with 2,4-dinitrobenzoic acid n-h ... | 1977 | 68463 |
| relationship between escherichia coli endotoxin toxicity and the immunization status of normal adult guinea-pigs. | lipopolysaccharide extracts of e. coli (endotoxins) were toxic to normal adult guinea-pigs, yet no evidence was found of serological reactivity to endotoxins or of "immediate" dermal reactivity in vivo. in contrast, testing of the serum of experimentally immunized animals provided evidence of precipitins, agglutinins and haemolysins. it is concluded that endotoxin exerts toxic effects in the absence of significant antibody production. this suggests that endotoxin toxicity is independent of immun ... | 1977 | 68785 |
| [conjugation of bovine gammaglobulin to l-asparaginase (e. coli) (author's transl)]. | bovine gammaglobulin-l-asparaginase conjugates were prepared with an enzymic activity of about 30 iu/mg. glutardialdehyde was used as bifunctional reagent for the cross-linking in an optimal concentration of 0.16 mg/10 mg protein. the both components were found in the conjugates in a ratio of 1:1.5. the recovery was about 25% of the initial protein amounts. the antigenic specificity of the globulin component decreased up to 60%, the enzymic activity also up to 60% by the conjugation. | 1977 | 69393 |
| the effect of phenethyl alcohol on in vitro dna synthesis in escherichia coli. | the effect of phenethyl alcohol on dna synthesis was examined using several in vitro systems of escherichia coli h560; i.e., ether-treated cells, membrane fractions and folded chromosomes fortified with dna polymerase. in all systems, the incorporation of deoxyribonucleotides was much reduced for the phenethyl alcohol-treated cells compared with the non-treated cells. the total activity of dna polymerases in pola1 cells (mostly dna polymerase ii) was not impaired for the phenethyl alcohol-treate ... | 1977 | 69442 |
| transformation of 5-aza-2'-(3h)deoxycytidine in escherichia coli. | | 1977 | 69550 |
| antigenic similarity of heat-labile enterotoxins from diverse strains of escherichia coli. | with use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either e. coli or vibrio cholerae. these findings suggest marked immunological similarity among heat-labile enterotoxins from a heterogenous group of e. coli. | 1977 | 69630 |
| two escherichia coli fructose-6-phosphate kinases. preparative purification, oligomeric structure and immunological studies. | two isoenzymes of fructose-6-phosphate kinase (atp: d-fructose-6-phosphate 1-phosphotransferase, ec 2.7.1.11) are present in escherichia coli k12. one isoenzyme is allosterically inhibited by phosphoenolpyruvate and activated by nucleoside diphosphates, and is a tetramer composed of four subunits of molecular weight 35 000. a simple method for the purification of this enzyme is reported. equilibrium dialysis indicates that there are four atp sites and four gdp sites per tetramer. the second isoe ... | 1977 | 70226 |
| quantitative granulocyte chemiluminescence in the rapid detection of impaired opsonization of escherichia coli. | previous work has demonstrated that 40% of clinically isolated escherichia coli are resistant to in vitro killing by normal human polymorphonuclear granulocytes (pmn) due to ineffective opsonophagocytosis. using these e. coli isolates, we have demonstrated the usefulness of measuring the chemiluminescence (cl) of granulocytes undergoing phagocytosis in detecting this impaired opsonization. cl correlated well with several other methods to assess pmn function including in vitro killing assays, pos ... | 1977 | 70407 |
| protective capacity of antibodies against escherichia coli and k antigens. | antibodies to escherichia coli o and k antigens were raised in rabbits by repeated immunizations with whole, formalin-killed and, later, liver bacteria. the serum antibody levels were determined with the ammonium sulfate precipitation technique after radioiodinating the antigens. the k antigens had to be conjugated to proteins before labeling. such conjugations were performed using cyanogen bromide for the k1 antigen and bisdiazobenzidine for the k13 antigen. the protective capacities of the rab ... | 1977 | 70410 |
| a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal infection. | as a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ... | 1977 | 70492 |
| differential staining of bacteria in clinical specimens using acridine orange buffered at low ph. | optimal conditions for acridine orange staining of air dried and methanol fixed bacteria on glass slides were studied. the ph of the staining buffer did not influence the fluorescence of an s. aureus and an e. coli strain at dye concentrations of 25-50 mg per litre. 81 bacterial strains representing 15 different species were stained with acridine orange under standard conditions, all strains showing orange fluorescence. the ph of the buffer influenced markedly the staining patterns of human cell ... | 1977 | 70957 |
| the transfer of bacterial biotin genes to escherichia coli k-12 cells by transfection with lambda bio phage dna. | the transfection of biotin genes to biotin negative escherichia coli k-12 cells with donor dna isolated from transducing lambda bio phages was examined. the transfection of the cells was performed in the presence of bovine serum albumin and sucrose (osowiecki and skalińska, 1974). | 1977 | 70968 |
| immunologic evidence for structural homology between the release factors of escherichia coli. | | 1977 | 71019 |
| 3'-terminal oligocytidylate addition to bacteriophage rna, using an adenylyltransferase from escherichia coli. | | 1976 | 71059 |
| protein-rna interactions in the escherichia coli ribosome: l24 protein-23 s rna complex. | | 1976 | 71068 |
| induction of alkaline phosphatase in escherichia coli. effect of phenethyl alcohol. | induction of alkaline phosphatase, an enzyme located in the periplasmic region of escherichia coli, was inhibited by phenethyl alcohol, an agent believed to alter the cell membrane structure. studies to elucidate mechanism of this inhibition showed that while phenethyl alcohol arrested the incorporation of [3h]leucine into active alkaline phosphatase, it did allow substantial incorporation of the label into inactive monomer subunits of the enzyme. these results suggest that phenethyl alcohol may ... | 1977 | 71163 |
| [antigenic properties of genetic recombinants of serologically typed e. coli]. | the conjugation between the typed strains of e. coli belonging to various serological groups and conjugation between typed and untyped e. coli strains were studied. genetic determinant controlling the synthesis of the o100 antigen proved to be closely linked with histidine locus. among recombinants obtained in crossing the typed e. coli strains there were such belonging to the serological type different from the serological types of donor and recipient cells. | 1977 | 71171 |
| isolation of enterotoxigenic aeromonas from fish. | this paper reports two different enterotoxigenic classes of the genus aeromonas isolated from fishes. strains of a. sobria were isolated from healthy fishes only wheras strains of a. hydrophila were isolated from both healthy and moribund fishes. aeromonas sobria and hydrophilia strains produced a cytotoxic factor and were highly proteolytic. seventy-five percent (75%) of the a. sobria strains produced only one type of hemolysis on 5% blood agar, but 92% of the a. hydrophilia produced two types ... | 1977 | 71190 |
| effect of heat on antigenicity and immunogenicity of the antigenic determinant shared by haemophilus influenzae type b and escherichia coli k100. | escherichia coli k100 produces an antigenic determinant similar to, or identical with, the capsular antigen of haemophilus influenzae type b. studies of the effects of heat on the immunogenicity, erythrocyte-modifying capacity, and antigenicity of this cross-reacting antigen (cra) revealed the following findings. immunization of rabbits with viable or formaldehyde-killed suspensions of e. coli k100, producing cra, engendered cra antibodies in significant titers, as demonstrated by hemagglutinati ... | 1977 | 71269 |
| [use of the indicator paper for isolation of e. coli from the wash water]. | | 1977 | 71382 |
| [immunological changes during hydrotherapy]. | immunological tests were performed in 34 patients undergoing hydrotherapy according to kneipp. igm, alpha2-macroglobulin and complement factor c3 concentrations were increased after this treatment, but not in ten healthy volunteers. specific antibody activities (staphylococci, e. coli, streptococci) remained unchanged. after intracutaneous testing with different protein and bacterial antigens reactions were significantly more intense (diameter of reaction) after hydrotherapy, while total number ... | 1977 | 72641 |
| antibiotic-induced colitis implication of a toxin neutralised by clostridium sordellii antitoxin. | a toxin(s) has been demonstrated in the stools of two patients with antibiotic-associated colitis. this toxin(s) was heat-labile, was rapidly lethal for hamsters, increased vascular permeability in rabbit skin, and was cytotoxic for cells in tissue-culture. it was neutralised by clostridium sordellii antitoxin but not by antitoxins prepared against other clostridia; escherichia coli, and vibrio cholerae toxins. these characteristics were identical to those of a toxin implicated in the aetiology ... | 1977 | 73011 |
| escherichia coli receptors on human kidney brush-border membranes. | | 1977 | 73094 |
| differences in antigenicity of escherichia coli strains isolated from patients with various forms of urinary tract infections. | the antibody amounts to crude o antigens were analyzed using the enzyme-linked immunosorbent assay (elisa) and the indirect hemagglutination (iha) technique in 42 sera from 6 rabbits. the rabbits were hyperimmunized with one of the who escherichia coli standard strains o1, o2 or o75. in the antibody assays the o antigens were obtained from e. coli strains isolated from 15 patients with asymptomatic bacteriuria (abu), from 3 patients with acute pyelonephritis and from the who e. coli strains use ... | 1977 | 73517 |
| amplifications of phleomycin and bleomycin-induced antibiotic activity in escherichia coli by aromatic cationic compounds. | a wide range of aromatic compounds has been shown to amplify phleomycin-induced cell killing in escherichia coli. they include acridines, acridinium chlorides, dihydroanthracenes, anthracenes, dianthracenes, phenanthridinium salts, phenazinium chlorides, phenoxazones, triphenyl methane dyes, benzoquinolizinium chloride, diphenylmethane derivatives, stilbene and diphenyl derivatives. low concentrations of these amplifiers also amplified the dna breakage and degradation effects of phleomycin. the ... | 1977 | 73539 |
| enterotoxigenic escherichia coli and reovirus-like agent in rural bangladesh. | 48 patients admitted to a rural bangladesh hospital with dehydration secondary to diarrhea were examined for infection caused by (r.l.a.) reovirus-like agent or (e.t.e.c.) enterotoxigenic escherichia coli. the diagnosis of r.l.a. infection was established by electron microscopy of stool filtrates and by a 4-fold or greater rise in serum complement-fixing antibodies to the nebraska calf diarrhea virus. evidence of infection by heat-labile-toxin producing e.t.e.c. was sought by stool culture and ... | 1976 | 73638 |
| secretory iga against enterotoxins in breast-milk. | a pool of colostrum from guatemalan mothers (guatemalan colostrum)) obtained 2-4 days post partum inhibited the induced fluid accumulation in rabbit ileal loops when incubated with vibrio cholerae or escherichia coli enterotoxin. there was a linear relationship between the quantity of colostrum used and the protection achieved. pools of guatemalan breast-milk obtained 15-30 days post partum and north american breast-milk had the same effect when tested with e. coli and v. cholerae enterotoxins, ... | 1976 | 73692 |
| enterotoxigenic e. coli in bangladesh. | | 1976 | 73728 |
| recognising epidemic strains of e. coli. | | 1976 | 73729 |
| variable adherence to normal human urinary-tract epithelial cells of escherichia coli strains associated with various forms of urinary-tract infection. | the ability to become attached to normal epithelial cells from the urinary tract was much greater in escherichia coli bacteria isolated from the urine of patients with acute symptomatic pyelonephritis or cystitis than in those isolated from the urine of patients with asymptomatic bacteriuria. attachment of the bacteria could be prevented by incubation in urine containing antibodies against the strain tested. the ability to attach to uroepithelial cells might be a virulence factor for e. coli str ... | 1976 | 74461 |
| the requirement of specific antibody for the killing of e. coli by the alternate complement pathway in bovine serum. | experiments using the selective divalent cation chelator, ethylene glycol tetraacetic acid (egta) showed that the alternate complement pathway was involved in the bactericidal action of bovine serum on two strains of e. coli. the bactericidal system was shown, in experiments involving egta and epsilon-amino caproic acid, to be independent of ca++ and the c1 unit of complement. the fixation of the complement components c3-c9 by endotoxin does not require a functional classical pathway ((c1, c4 an ... | 1978 | 75177 |
| immunological aspects of cell membrane adhesion demonstrated by porcine enteropathogenic escherichia coli. | | 1978 | 75179 |
| [use of the erythrocytes, sensitized with antibodies, for diagnosis of escherichia coli infection]. | | 1978 | 75295 |
| mechanism of conjugation and recombination in bacteria. xviii. polarity of donor dna strands transferred to the recipient as determined by dna-rna hybridization. | polarity of donor dna strand transferred into recipient during conjugation in escherichia coli k-12 was determined by dna-3h-rna hybridization. lambda prophage was used as a marker. the defective lysogen hfr h (lambdat11) as a donor and thermosensitive f- cr34 dnab strain as recipient were used. two sets of hybridization experiments, with 1-strand specific lambda mrna and lambda mrna specific for both phage strands but with large excess of r-strand specific mrna, were carried out. strand 1 of la ... | 1977 | 75660 |
| genetic properties of the salmonella enteritidis r404 plasmid aggregate i. conjugational separation of different derivative plasmid aggregates and their genetic properties. | conjugational transfer of r404 factor, found in salmonella enteritidis strain, was examined. six derivative forms differing in resistance pattern were isolated. the manner of conjugational separation during transfer of r404 factor from e. coli to e. coli strain was the same as from the original strain of s. enteritidis to e. coli strain. during following conjugation, all isolated forms except one, could give forms differing in a resistance pattern from the parenteral ones. this one form, from wh ... | 1977 | 75661 |
| solid-phase radioimmunoassay method for determination of escherichia coli enterotoxin. | the development of a solid-phase radioimmunoassay procedure for the determination of escherichia coli enterotoxin(s) is described. radioiodinated e. coli enterotoxin with about three radioiodine atoms per toxin molecule is, by the criterion of electrophoresis, identical to the unlabeled toxin. goat anti-e. coli-enterotoxin antibody was coupled to polystyrene tubes and served as a solified toxin binder in the reported procedure. various conditions necessary for the optimization and standardizatio ... | 1978 | 75844 |
| synthesis of double-stranded dna complementary to lysozyme, ovomucoid, and ovalbumin mrnas. optimization for full length second strand synthesis by escherichia coli dna polymerase i. | sequential reverse transcriptase, dna polymerase, and s1 nuclease reactions can be employed to synthesize double-stranded dna representing messenger rna. using reverse transcriptase products made from partially purified lysozyme, ovomucoid, and ovalbumin messengers from hen oviduct, we have characterized the escherichia coli dna polymerase i reaction. we have optimized for a high yield of full length second strands under conditions which require only a small amount of mrna. the effects of severa ... | 1978 | 75887 |
| immunologic responsiveness of the c3h/hej mouse: differential ability of butanol-extracted lipopolysaccharide (lps) to evoke lps-mediated effects. | the lipopolysaccharide (lps)-protein complex extracted from the cell wall of escherichia coli k235 by the butanol-water technique has been shown to evoke a mitogenic response in bone marrow-derived (b) lymphocytes from the c3h/hej mouse strain. these mice are resistant to the effects of lps extracted with phenol. therefore, the ability of butanol-extracted lps to modulate a spectrum of c3h/hej b-cell functions was investigated. both butanol-extracted (lps-b) and phenol-extracted (lps-p) lps prep ... | 1978 | 75941 |
| [resorption of colostral gamma-globulins and detection of antibodies to bact. rhusiopathiaesuis, s. cholerasuis and beta-hemolytic esch. coli in newborn pigs]. | traced was the resorption of colostral globulins and the production of antibodies against bacterium rhusiopathiae suis, s. choleraesuis, and beta-hemolytic escherichia coli organisms in newborn pigs. it was found that in the newborn pigs that had not yet started sucking there were no gamma-globulins and specific antibodies against the agents mentioned above. such were observed after the animals had begun to suck, the titers of antibodies reaching their peak levels by the 10th--15th hour (erysipe ... | 1977 | 76366 |
| plasmid borne virulence of enterobacter hafniae. | the strain classified as enterobacter hafniae has been isolated in a severe epidemy of porcine diarrhoea. its pathogenicity has been found to be cotransmissible with resistance to oxytetracycline when the strain was grown in a mixed culture with some nonpathogenic e. hafniae strains as well as with escherichia coli. toxinogenity seems to be responsible for virulence of the strain. | 1978 | 76419 |
| immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients. | by the use of specific antibody to human chorionic gonadotropin (cg) as well as to its beta-subunit, and the application of the indirect fluorescein-labeled and peroxidase-labeled antibody techniques, we have demonstrated the presence of a membrane (wall)-associated cg-similar immunoreactive protein in 15 strains of bacteria isolated from tissues of patients bearing malignant neoplasms. these microorganisms were classified as s. epidermidis, (12) e. coli (2), and a single strain of p. maltophili ... | 1978 | 76504 |
| interaction of 4'-(9-acridinylamino)methanesulfon-m-anisidide with dna and inhibition of oncornavirus reverse transcriptase and cellular nucleic acid polymerases. | 4'-(9-acridinylamino)methanesulphon-m-anisidide (amsa) (nsc 141549), an acridine derivative with activity against a variety of laboratory tumors in vivo, is presently undergoing phase 1 clinical evaluation. the interaction of amsa with dna and its effects on nucleic acid-polymerizing enzymes were examined in an attempt to define the site of cytotoxicity of amsa. binding of amsa to dna, as demonstrated by equilibrium dialysis and spectrophotometric methods, appears to be similar to other aminoacr ... | 1978 | 76512 |