| sensitivity of 341 non-fermentative gram-negative bacteria to seven beta-lactam antibiotics. | susceptibility of 341 isolates of non-fermentative gram-negative bacteria to carbenicillin, piperacillin, cefoperazone, moxalactam, cefotaxime, ceftizoxime, and n-formimidoyl thienamycin was determined by the agar dilution and disc diffusion methods. piperacillin was the most active agent against pseudomonas aeruginosa, thienamycin the most active against pseudomonas fluorescens and pseudomonas putida, and moxalactam the most active against pseudomonas maltophilia. piperacillin and thienamycin w ... | 1982 | 7173181 |
| behavior of enterococci in egg processing operations. | since enterococci were detected in dried and frozen egg products (whole egg and egg yolk), the origin of the enterococci and their behavior in the different stages of egg processing were surveyed. the bacterial survey of unwashed and washed eggs, gathered from several parts of japan, showed the presence of an average of 60 enterococci per egg on the shell surface of unwashed eggs. smaller numbers of enterococci were detected on the shell surface of washed eggs. most of the detected enterococci w ... | 1980 | 7413584 |
| relationship of primer specificity of fatty acid de novo synthetase to fatty acid composition in 10 species of bacteria and yeasts. | fatty acid compositions of lipids from six bacteria and four yeasts were determined. fatty acid de novo synthetases were investigated with respect to chain length specificity towards acyl-coa primers of various chain lengths. four species of bacteria (bacillus subtilis, corynebacterium cyclohexanicum, micrococcus luteus, and pseudomonas maltophilia) possess branched-chain fatty acids of the iso and anteiso series as the major acids. de novo synthetases from these organisms exhibited specificity ... | 1980 | 7459715 |
| substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates). | the substrate specificities of extracellular lipases purified from bacillus subtilis, pseudomonas aeruginosa, pseudomonas alcaligenes, pseudomonas fluorescens, and burkholderia cepacia (former pseudomonas cepacia) and of extracellular polyhydroxyalkanoate (pha) depolymerases purified from comamonas sp., pseudomonas lemoignei, and p. fluorescens gk13, as well as that of an esterase purified from p. fluorescens gk 13, to various polyesters and to lipase substrates were analyzed. all lipases and th ... | 1995 | 7487042 |
| pollution of modern metalworking fluids containing biocides by pathogenic bacteria in france. reexamination of chemical treatments accuracy. | pollution by pathogenic bacteria was examined in 150 french metalworking fluid samples. gram-negative micro-organisms such as salmonella spp., shigella spp., and vibrio spp. as well as gram-positive cocci were never isolated. nevertheless opportunistic pathogens such as pseudomonas aeruginosa and klebsiella pneumoniae still contaminated these fluids with an isolation frequency of 17% of samples for each. these two micro-organisms failed to grow or even survive in vitro in sterile cutting fluids ... | 1995 | 7489767 |
| purification and characterization of acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in escherichia coli. | 4-hydroxybenzoate 3-hydroxylase [ec 1.14.13.2] from acinetobacter calcoaceticus was purified to homogeneity following the 40-fold overexpression of this gene (poba) in escherichia coli. overexpression was accomplished by placing the fola gene (encoding trimethoprim-resistant dihydrofolate reductase) directly downstream of the poba gene, and demanding growth of recombinants on elevated concentration of trimethoprim. presumably, the surviving variants have undergone a genetic alteration which allo ... | 1995 | 7490269 |
| novel cellulose-binding domains, nodb homologues and conserved modular architecture in xylanases from the aerobic soil bacteria pseudomonas fluorescens subsp. cellulosa and cellvibrio mixtus. | to test the hypothesis that selective pressure has led to the retention of cellulose-binding domains (cbds) by hemicellulase enzymes from aerobic bacteria, four new xylanase (xyn) genes from two cellulolytic soil bacteria, pseudomonas fluorescens subsp. cellulosa and cellvibrio mixtus, have been isolated and sequenced. pseudomonas genes xyne and xynf encoded modular xylanases (xyle and xylf) with predicted m(r) values of 68,600 and 65000 respectively. xyle contained a glycosyl hydrolase family 1 ... | 1995 | 7492333 |
| the conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. | two cdnas, designated xyna and mana, encoding xylanase a (xyla) and mannanase a (mana), respectively, were isolated from a cdna library derived from mrna extracted from the anaerobic fungus, piromyces. xyla and mana displayed properties typical of endo-beta 1,4-xylanases and mannanases, respectively. neither enzyme hydrolyzed cellulosic substrates. the nucleotide sequences of xyna and mana revealed open reading frames of 1875 and 1818 base pairs, respectively, coding for proteins of m(r) 68,049 ... | 1995 | 7493964 |
| iron-regulated salicylate synthesis by pseudomonas spp. | two iron-regulated compounds have been found in acidified ethyl acetate extracts from culture supernatants of pseudomonas aeruginosa and pseudomonas cepacia type-strains. synthesis of both compounds paralleled iron-deficient growth, and was repressed in the presence of 100 microm-fecl3. yields of these substances varied among different strains and attained maximum levels during stationary phase. thin layer chromatographic analysis in five different solvent systems revealed that the slower-moving ... | 1993 | 7504066 |
| use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates. | a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ... | 1993 | 7504429 |
| growth of mycorrhizal fungi in dixenic cultures with bacteria in media of different composition. | studies were carried out on the effect of bacteria: arthrobacter globiformis, bacillus subtilis and pseudomonas fluorescens on biomass production by three important ectomycorrhizal fungi: laccaria laccata, hebeloma crustuliniforme and rhizopogon vinicolor in media of different composition. it was shown that bacteria stimulated, inhibited or did not affect significantly the biomass production by mycorrhizal fungi. 3-factor anova have shown that although effect of bacteria was statistically signif ... | 1993 | 7504874 |
| characterization of contaminating dna in taq polymerase which occurs during amplification with a primer set for legionella 5s ribosomal rna. | an amplification product that occurred in negative controls of a pcr using a primer system for legionella 55 ribosomal rna was characterized by direct sequencing. the amplification product did not hybridize to a legionella specific oligonucleotide. it was derived from bacterial dna contaminating taq dna polymerase, a phenomenon that was previously reported for amplification reactions with universal primer sets for bacterial 16s rrna. the sequence of the 5s ribosomal fragment had close homology t ... | 1994 | 7518037 |
| somatic antigens of pseudomonads: structure of the o-specific polysaccharide of pseudomonas fluorescens biovar a strain imv 472. | | 1994 | 7518743 |
| crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate,2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate and of the tyr222ala mutant complexed with 2-hydroxy-4-aminobenzoate. evidence for a proton channel and a new binding mode of the flavin ring. | the crystal structures of wild-type p-hydroxybenzoate hydroxylase from pseudomonas fluorescens, complexed with the substrate analogues 4-aminobenzoate, 2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate have been determined at 2.3-, 2.5-, and 2.8-a resolution, respectively. in addition, the crystal structure of a tyr222ala mutant, complexed with 2-hydroxy-4-aminobenzoate, has been determined at 2.7-a resolution. the structures have been refined to r factors between 14.5% and 15.8% for data bet ... | 1994 | 7520279 |
| molecular characterization of the major outer-membrane protein oprf from plant root-colonizing pseudomonas fluorescens. | n-terminal sequence analysis of peptides generated by proteolytic treatment of the pseudomonas fluorescens oe 28.3 major outer-membrane protein oprf, embedded in outer membranes or present in whole cells, indicated a surface-exposed location for the proline-rich region of the protein. this region is absent from the p. aeruginosa and p. syringae oprfs. evidence was obtained for the presence of additional exposed but less accessible regions in the carboxy half of oprf. four oprf-specific monoclona ... | 1994 | 7521711 |
| pcr-based preparation of 23s rrna-targeted group-specific polynucleotide probes. | dna coding for a variable region within domain iii of bacterial 23s rrna was used as the target for group-specific polynucleotide hybridization probes. the corresponding rdna was amplified in vitro by the pcr technique in combination with a pair of primers specific for flanking conserved target sites. the amplified fragments were cloned or used directly as probes. rna probes were generated by in vitro transcription of cloned or amplified rdna. the probes were labeled by incorporating modified nu ... | 1994 | 7524442 |
| statistical analysis of joint toxicity in biological growth experiments. | the authors formulate a model for the analysis of designed biological growth experiments where a mixture of toxicants is applied to biological target organisms. the purpose of such experiments is to assess the toxicity of the mixture in comparison with the toxicity observed when the toxicants are applied individually to the organisms. the analysis is based on a random differential equation describing the growth of the organisms. this model yields a natural measure of interaction between toxicant ... | 1994 | 7525214 |
| effect of mixtures of chlorophenols, surfactants, and aniline on growth of pseudomonas fluorescens. | industrial wastewater often contains a mixture of chemical substances and knowledge of joint action of toxicants is therefore important when the toxicity of an effluent is evaluated or reduction of the toxicity is needed. in this study, the joint action of three pairs of toxicants, selected on the basis of their expected different modes of toxic actions, was tested for inhibition of the growth of the bacteria pseudomonas fluorescens: pentachlorophenol and aniline, the surfactants nonylphenoletho ... | 1994 | 7529167 |
| application of a strain-specific rrna oligonucleotide probe targeting pseudomonas fluorescens ag1 in a mesocosm study of bacterial release into the environment. | sequence analysis of domains 3 and 4 of 23s rrna from pseudomonas fluorescens ag1 was carried out to allow the design of a strain-specific rrna oligonucleotide probe targeting this strain. the specificity of the probe, ps-ag1, was assessed by dot blot analysis and whole-cell hybridization, and it was found to be specific for p. fluorescens ag1. the correlation between the ribosomal content of p. fluorescens ag1 and growth rate was determined during balanced growth conditions with generation time ... | 1995 | 7538276 |
| channel-forming properties and structural homology of major outer membrane proteins from pseudomonas fluorescens mfo and oe 28.3. | the major outer membrane proteins (oprf) from pseudomonas fluorescens mfo and oe 28.3 were purified by a new method involving native electrophoresis in octyl-polyoxyethylene media. both proteins, characterized by the same size, heat-modifiability and n-terminal sequence were re-incorporated in virtually solvent-free planar lipid bilayers. they displayed very similar channel-forming properties: the major conductance level was between 250 ps and 270 ps in 1 m nacl. from experiments of zero-current ... | 1995 | 7538961 |
| direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose. | gene libraries ("zoolibraries") were constructed in escherichia coli using dna isolated from the mixed liquor of thermophilic, anaerobic digesters, which were in continuous operation with lignocellulosic feedstocks for over 10 years. clones expressing cellulase and xylosidase were readily recovered from these libraries. four clones that hydrolyzed carboxymethylcellulose and methylumbelliferyl-beta-d-cellobiopyranoside were characterized. all four cellulases exhibited temperature optima (60-65 de ... | 1995 | 7546604 |
| occurrence of a furan fatty acid in marine bacteria. | a fatty acid containing a furan ring was detected in the cellular lipids of marine bacteria, shewanella putrefaciens, marinomonas comunis, enterobacter agglomerans, pseudomonas fluorescens, etc., which were isolated from the intestinal liquor of fishes. analytical data indicated that the fatty acid was 10,13-epoxy-11-methyloctadeca-10, 12-dienoic acid. therefore, we propose that furan fatty acids detected in marine fish are derived not only from marine plants but also from intestinal bacteria of ... | 1995 | 7548190 |
| rate of growth of pseudomonas fluorescens in donated blood. | to examine how delayed refrigeration of blood affects the growth of pseudomonas fluorescens, one of the two most important causes of sepsis resulting from transfusion of contaminated blood. | 1995 | 7560196 |
| identification of exopolysaccharides produced by fluorescent pseudomonads associated with commercial mushroom (agaricus bisporus) production. | the acidic exopolysaccharides (epss) from 63 strains of mushroom production-associated fluorescent pseudomonads which were mucoid on pseudomonas agar f medium (paf) were isolated, partially purified, and characterized. the strains were originally isolated from discolored lesion which developed postharvest on mushroom (agaricus bisporus) caps or from commercial lots of mushroom casing medium. an acidic galactoglucan, previously named marginalan, was produced by mucoid strains of the saprophyte ps ... | 1995 | 7574589 |
| microscale detection of specific bacterial dna in soil with a magnetic capture-hybridization and pcr amplification assay. | a magnetic capture-hybridization pcr technique (mch-pcr) was developed to eliminate the inhibitory effect of humic acids and other contaminants in pcrs targeting specific soil dna. a single-stranded dna probe, which was complementary to an internal part of the target gene, was used to coat magnetic beads. after hybridization in a suspension of soil dna, magnetic extraction of the beads separated the hybrid dna from all other soil dna, humic acids, and other interfering soil components. the mch w ... | 1995 | 7574645 |
| liquid-culture ph, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent pseudomonas fluorescens 2-79. | strain 2-79 is a biocontrol agent against take-all, an important disease of wheat caused by gaeumannomyces graminis var. tritici. in the rhizosphere, it produces the antibiotic phenazine 1-carboxylic acid (pca) as the primary means of disease suppression. one barrier to commercial use of phenazine-producing pseudomonads, like strain 2-79, is the lack of liquid-culture technology for mass production. for instance, there is little published research concerning the impact of liquid-culture secondar ... | 1995 | 7576546 |
| ornibactin production and transport properties in strains of burkholderia vietnamiensis and burkholderia cepacia (formerly pseudomonas cepacia). | several strains of burkholderia vietnamiensis, isolated from the rhizosphere of rice plants, and four strains formerly known as pseudomonas cepacia including two collection strains and two clinical isolates were compared for siderophore production and iron uptake. the b. vietnamiensis (tvv strains) as well as the b. cepacia strains (atcc 25416 and atcc 17759) and the clinical isolates k132 and lmg 6999 were all found to produce ornibactins under iron starvation. the two atcc strains of b. cepaci ... | 1995 | 7580051 |
| the phylogenetic distribution of a transposable dioxygenase from the niagara river watershed. | horizontal gene transfer in the bacteria has been demonstrated to occur under natural conditions. the ecological impact of gene transfer events depends on the new genetic material being expressed in recipient organisms, and on natural selection processes operating on these recipients. the phylogenetic distribution of cbaab genes for chlorobenzoate 3,4-(4,5)-dioxygenase, which are carried within tn5271 on the incp beta plasmid pbrc60, was investigated using isolates from freshwater microcosms and ... | 1995 | 7582167 |
| membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. | the ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. membrane hyperpolarisation in escherichia coli, pseudomonas fluorescens, enterobacter aerogenes and arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. dye uptake was variable both between species ... | 1995 | 7590182 |
| some factors affecting the airborne survival of bacteria outdoors. | airborne survival of two pseudomonads and a reference strain of escherichia coli (strain mre 162) was studied outdoors using a modified microthread technique. when cells of e. coli were suspended as clusters, survival was much greater than single cells, particularly outdoors. culture age had a highly significant effect on survival of pseudomonas maltophila with survival of 24 h cultures being more than 100-fold higher than 48 h cultures. survival of pseudomonas fluorescens was variable and depen ... | 1995 | 7592129 |
| a global regulator of secondary metabolite production in pseudomonas fluorescens pf-5. | mutations in the apda (for antibiotic production) gene of the plant root-colonizing bacterium pseudomonas fluorescens pf-5 pleiotropically abolish the production of an array of antibiotics, including pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol, as well as the production of tryptophan side chain oxidase, hydrogen cyanide, and an extracellular protease. the lack of production of secondary metabolites by apda- mutants was correlated with the loss of inhibition of the phytopathogenic f ... | 1995 | 7592389 |
| substrate specificities of poly(hydroxyalkanoate)-degrading bacteria and active site studies on the extracellular poly(3-hydroxyoctanoic acid) depolymerase of pseudomonas fluorescens gk13. | the isolation of poly(3-hydroxyoctanoic acid)- and poly(6-hydroxyhexanoic acid)-degrading bacteria yielded 28 strains with abilities to degrade various polymers. the most versatile strains hydrolyzed five different polyesters comprising short chain length and medium chain length poly(hydroxyalkanoates). the new isolates together with previously isolated poly(hydroxyalkanoate)-degrading bacteria were classified into 11 groups with respect to their polymer-degrading specificities. all pha depolyme ... | 1995 | 7606661 |
| gene cloning, sequence analysis, purification, and secretion by escherichia coli of an extracellular lipase from serratia marcescens. | the gene encoding extracellular lipase of serratia marcescens has been identified from a phage lambda genomic library. formation of orange-red fluorescent plaques on rhodamine b-triolein plates was used to identify phages carrying the lipase gene. a 2.8-kb sali fragment was subcloned into a plasmid, and lipase was expressed in escherichia coli. extracellular lipase was detected in the presence of the secretion plasmid pgsd6 carrying the genes prtd, -e, and -f, which guide the secretion of protea ... | 1995 | 7618881 |
| a catalytic triad is required by the non-heme haloperoxidases to perform halogenation. | the bacterial non-heme haloperoxidases are highly related to an esterase from pseudomonas fluorescens, at structural and functional levels. both types of enzymes displayed brominating activity and esterase activity. the presence of the serine-hydrolase motif gly-x-ser-x-gly, in the esterase as well as in all aligned haloperoxidase sequences, strongly suggested that they belong to the serine-hydrolase family. sequence alignment with several serine-hydrolases and secondary structure superimpositio ... | 1995 | 7632719 |
| the non-catalytic cellulose-binding domain of a novel cellulase from pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of avicel. | a genomic library of pseudomonas fluorescens subsp. cellulosa dna, constructed in lambda zapii, was screened for carboxymethyl-cellulase activity. the pseudomonad insert from a recombinant phage which displayed elevated cellulase activity in comparison with other cellulase-positive clones present in the library, was excised into pbluescript sk- to generate the plasmid pc48. the nucleotide sequence of the cellulase gene, designated cele, revealed a single open reading frame of 1710 bp that encode ... | 1995 | 7639689 |
| construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere. | the genetically engineered transposon tnpcb, contains genes (bph) encoding the biphenyl degradative pathway. tnpcb was stably inserted into the chromosome of two different rhizosphere pseudomonads. one genetically modified strain, pseudomonas fluorescens f113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. the expression of the heterologous bph genes in f113pcb was confirmed by the ability of the genet ... | 1995 | 7646029 |
| isolation of ice-nucleating active bacteria from the freeze-tolerant frog, rana sylvatica. | ice-nucleating active (ina) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (rana sylvatica) collected in winter. thirteen strains of pseudomonas fluorescens, four strains of pseudomonas putida, and two strains of enterobacter agglomerans had ice-nucleating activity. each of the ina pseudomonad strains was psychrophilic. p. putida strains were differentiated from p. fluorescens strains by gelatinase, lecithinase, and lipase production. the maximum nucleation tem ... | 1995 | 7656570 |
| machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. | six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ... | 1995 | 7656609 |
| amplification of the housekeeping sigma factor in pseudomonas fluorescens cha0 enhances antibiotic production and improves biocontrol abilities. | pseudomonas fluorescens cha0 produces a variety of secondary metabolites, in particular the antibiotics pyoluteorin and 2,4-diacetylphloroglucinol, and protects various plants from diseases caused by soilborne pathogenic fungi. the rpod gene encoding the housekeeping sigma factor sigma 70 of p. fluorescens was sequenced. the deduced rpod protein showed 83% identity with rpod of pseudomonas aeruginosa and 67% identity with rpod of escherichia coli. attempts to inactivate the single chromosomal rp ... | 1995 | 7665535 |
| lipoprotein lipase enhances removal of chylomicrons and chylomicron remnants by the perfused rat liver. | lipoprotein lipase has been found to efficiently mediate binding of lipoproteins to cell surfaces and to the low density lipoprotein (ldl) receptor-related protein (lrp) under cell culture conditions (beisiegel et al. 1991. proc. natl. acad. sci. usa. 88: 8242-8346). this supports the previously proposed idea that the lipase could have a role in receptor-mediated uptake of chylomicron remnants in the liver. we have investigated the effects of lipoprotein lipase on the clearance of chylomicrons d ... | 1995 | 7666010 |
| comparison of bacterial toxicity tests based on growth, dehydrogenase activity, and esterase activity of pseudomonas fluorescens. | bacterial test systems that are used to assess the environmental risk of chemicals show considerable differences in sensitivity to toxic chemicals. this variation may be caused by differences in sensitivity between species and test parameters of the test systems. in this study, the differences in sensitivity of three test parameters, growth, esterase activity, and dehydrogenase activity, using pseudomonas fluorescens as the test organism, were studied. the effect of eight substances was tested: ... | 1993 | 7682916 |
| characterization and sequence of a novel insertion sequence, is1162, from pseudomonas fluorescens. | we have determined the nucleotide sequence of is1162, a new insertion sequence (is) isolated from pseudomonas fluorescens (pf) strain st. this is element is present in two copies on the peg plasmid harboured by pf st and in a single copy on the chromosome, adjacent to the styrene catabolic genes. is1162 is 2634 bp in length with 12-bp terminal inverted repeats (ir), and could encode four proteins (orfs), two for each strand. one strand, pro1 (62,990 da), showed a helix-turn-helix motif at the n- ... | 1995 | 7698671 |
| a bacterial esterase is homologous with non-haem haloperoxidases and displays brominating activity. | screening genbank indicated that an esterase from pseudomonas fluorescens had high sequence similarity with bacterial non-haem haloperoxides. however, this homology was limited to two distinct domains of the published esterase sequence. as errors in the published sequence were suspected, the esterase gene was sequenced again. the revised sequence displayed between 40 and 50% identical amino acids with the haloperoxidases, but distributed along the whole sequence. in addition to the structural ho ... | 1995 | 7704276 |
| [microbial sensitivity spectrum of framycetin. comparison with the 1972 to 1993 resistance status]. | framycetin was tested against a variety of isolates of grampositive and gramnegative bacteria. the in-vitro activity of framycetin against staphylococcus aureus, enterobacteriaceae, pseudomonas aeruginosa as well as pseudomonas fluorescens is today still favourable. | 1995 | 7709496 |
| mechanisms of biodegradation of metal-citrate complexes by pseudomonas fluorescens. | biodegradation of metal-citrate complexes by pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. bidentate fe(iii)-, ni-, and zn-citrate complexes were readily biodegraded, but the tridentate cd- and cu-citrate, and u-citrate complexes were not. the biodegradation of ni- and zn-citrate commenced after an initial lag period; the former showed only partial (70%) degradation, whereas the latter was completely degraded. uptake studies with 14c-label ... | 1995 | 7721690 |
| conjugative transposition of tn916-related elements from enterococcus faecalis to escherichia coli and pseudomonas fluorescens. | we studied the ability of transposons tn916, tn1545, and tn916-km, a tn916 derivative expressing kanamycin resistance, to be conjugatively transferred from enterococcus faecalis to various gram-negative bacteria. our results demonstrate that these types of elements can carry out conjugative transposition from the chromosome of e. faecalis to those of escherichia coli and pseudomonas fluorescens and that the accomplishment of this event depends on the donor potential of the e. faecalis transposon ... | 1995 | 7726521 |
| comparative ribosomal protein sequence analyses of a phylogenetically defined genus, pseudomonas, and its relatives. | i analyzed various families of ribosomal proteins obtained from selected species belonging to the genus pseudomonas sensu stricto and allied organisms which were previously classified in the genus pseudomonas. partial amino acid sequencing of l30 preparations revealed that the strains which i examined could be divided into three clusters. the first cluster, which was assigned to the genus pseudomonas sensu stricto, included pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, and p ... | 1995 | 7727274 |
| [the destruction of mono- and polycyclic aromatic hydrocarbons by cultures of pseudomonas fluorescens 1-d biovar ii and bacillus subtilis 2-d]. | cenosis of microorganisms, coal resin destructors, is selected by the percolation method. a microbiological analysis of bacterial associations able to destruction of aromatic hydrocarbons composing the coal resin is carried out. pure cultures are isolated as the most active destructors of these substrates. destructive cultures are identified as pseudomonas fluorescens 1-d biovar ii and bacillus subtilis 2-d. efficiency of the microbiological refinement of coal-resin-contaminated soil by the isol ... | 1995 | 7728279 |
| beta-glucosidase, beta-galactosidase, family a cellulases, family f xylanases and two barley glycanases form a superfamily of enzymes with 8-fold beta/alpha architecture and with two conserved glutamates near the carboxy-terminal ends of beta-strands four and seven. | comparison of the recently determined crystal structures pseudomonas fluorescens subsp. cellulosa family f xylanase, (1-3)-beta-glucanase and (1-3,1-4)-beta-glucanase and the catalytic domain of e. coli beta-galactosidase reveals that they belong to a superfamily of 8-fold beta/alpha-barrels with similar amino acid residues at their active sites. in the three families that these enzymes represent, the nucleophile is a glutamate, which is located close to the carboxy-terminus of beta-strand seven ... | 1995 | 7729513 |
| cloning and nucleotide sequence of the signal peptidase ii (lsp)-gene from staphylococcus carnosus. | staphylococcus carnosus tm300 is able to synthesize at least seven lipoproteins with molecular masses between 15 and 45 kda; the proteins are located in the membrane fraction. it can be concluded that this strain also posesses the enzymes involved in lipoprotein modification and prolipoprotein signal peptidase (signal peptidase ii) processing. the gene encoding the prolipoprotein signal peptidase, lsp, from staphylococcus carnosus tm300 was cloned in escherichia coli and sequenced. the deduced a ... | 1995 | 7729667 |
| degradation of triglycerides by a pseudomonad isolated from milk: the roles of lipase and esterase studied using recombinant strains over-producing, or specifically deficient in these enzymes. | the roles of lipase and esterase in causing hydrolytic spoilage of milk by a highly lipolytic psychrotrophic strain of pseudomonas fluorescens, ls107d2, has been studied. strains of ls107d2 have been constructed that over-produce, or are specifically deficient in, a lipase (encoded by lipa) and an esterase (encoded by esta). southern blot analysis reveals that ls107d2 contains only one esterase and one lipase (encoded by esta and lipa) and this was confirmed by the phenotypes of mutants on triol ... | 1995 | 7730200 |
| iron-responsive gene expression in pseudomonas fluorescens m114: cloning and characterization of a transcription-activating factor, pbra. | in response to iron limitation. pseudomonas fluorescens m114 induces a number of genes including an iron-scavenging siderophore termed pseudobactin m114, its cognate receptor, pbua, and a casein protease. a tn5lacz-induced mutant (m114fa1) was isolated that exhibits a pleiotropic phenotype and lacks the ability to express these iron-regulated genes. a cosmid clone was identified which complements this mutation. this clone is capable of activating a number of iron-regulated promoter fusion constr ... | 1995 | 7746151 |
| effect of impact stress on microbial recovery on an agar surface. | microbial stress due to the impaction of microorganisms onto an agar collection surface was studied experimentally. the relative recovery rates of aerosolized pseudomonas fluorescens and micrococcus luteus were determined as a function of the impaction velocity by using a moving agar slide impactor operating over a flow rate range from 3.8 to 40 liters/min yielding impaction velocities from 24 to 250 m/s. as a reference, the sixth stage of the andersen six-stage viable particle sizing sampler wa ... | 1995 | 7747946 |
| crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin. | the flavin prosthetic group (fad) of p-hydroxybenzoate hydroxylase from pseudomonas fluorescens was replaced by a stereochemical analog, which is spontaneously formed from natural fad in alcohol oxidases from methylotrophic yeasts. reconstitution of p-hydroxybenzoate hydroxylase from apoprotein and modified fad is a rapid process complete within seconds. crystals of the enzyme-substrate complex of modified fad-containing p-hydroxybenzoate hydroxylase diffract to 2.1 a resolution. the crystal str ... | 1994 | 7756982 |
| bacterial assay for quantitative measurement of nanomolar concentrations of a metabolite. | a mutant of pseudomonas fluorescens was used to develop a radioligand, competitive binding assay to quantitatively measure gamma-aminobutyric acid. the highly reliable and reproducible assay was sensitive (nm detection), rapid, and easy to perform. nonspecific activity and scatter were insignificant. radiolabel was irreversibly fixed by the cells in an energy-dependent reaction. the finding that a bacterium was effective in quantitatively detecting nanomolar amounts of a metabolite suggests that ... | 1995 | 7762792 |
| determination of gamma-aminobutyric acid levels in human cerebrospinal fluid using pseudomonas. | the principle objective was to demonstrate the efficacy of a bacterial, radioligand, competitive binding method in determining gamma-aminobutyric acid (gaba) levels in human cerebrospinal fluid (csf). in a double blind study, csf gaba concentrations were measured by the bacterial method using a mutant of pseudomonas fluorescens and by a standard radioligand competitive binding assay using rat brain membranes. linear regression analysis demonstrated a highly significant correlation (r = 0.84; p < ... | 1995 | 7762793 |
| thermolabile alanine racemase from a psychotroph, pseudomonas fluorescens: purification and properties. | a psychotrophic bacterium that produces a thermolabile alanine racemase was isolated from raw milk, and identified as pseudomonas fluorescens tm5-2. the enzyme was purified to homogeneity from the cell extract, and characterized to be compared with enzymes from mesophiles (bacillus subtilis and salmonella typhimurium) and a thermophile (bacillus stearothermophilus). the enzyme has a molecular weight of about 76,000 and consists of two subunits identical in molecular weight (38,000). the enzyme c ... | 1993 | 7763424 |
| applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. | lipases from candida cylindracea, aspergillus niger, and pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium. the enzyme preparations were tested in the transesterification of triolein with lauric acid and the esterification of lauric acid with different alcohols. immobilized c. cylindracea preparations were more active when hexane was used as the reaction medium, and anion-exchange resin was a better s ... | 1993 | 7763454 |
| cloning of pseudomonas fluorescens carboxylesterase gene and characterization of its product expressed in escherichia coli. | a gene (estc) coding for an esterase (esterase iii) of pseudomonas fluorescens was cloned into escherichia coli jm83. dna sequencing showed a single open reading frame with gtg as a translation initiation codon for esterase iii. this was confirmed by n-terminal amino acid sequence analysis of the purified esterase iii protein from an e. coli clone. the promoter sequence and a potential shine-dalgarno sequence were followed by the coding sequence of the estc gene. the amino acid sequence deduced ... | 1994 | 7764506 |
| cloning and sequencing of an ice nucleation active gene of erwinia uredovora. | an ice nucleation activity gene, named inau, of the bacterium erwinia uredovora kuin-3 has been sequenced. this gene encodes a protein of 1034 amino acid residues, and its expression product, inau protein, has an 832-amino acid residue segment consisting of 52 repeats of closely related 16-amino acid motifs (r-domain), flanked by n- and c-terminal sequences (n- and c-domains, respectively). the primary structure of the inau protein is similar to those of the inaa, inaw, and inaz gene products of ... | 1994 | 7764866 |
| pseudomonas fluorescens lipase adsorption and the kinetics of hydrolysis in a dynamic emulsion system. | to elucidate the adsorption characteristics of lipases and to study the influence of the reaction conditions on the catalytic properties of lipases, the hydrolysis of decylchloroacetate by pseudomonas fluorescens lipase in an emulsion reactor was studied as a model system. during the reaction the droplet size distribution of the emulsion was measured on-line using a particle sizer based on light scattering. desorption experiments revealed that, at low surface coverage, the initial rate of reacti ... | 1994 | 7764891 |
| metabolic diversity of aromatic hydrocarbon-degrading bacteria from a petroleum-contaminated aquifer. | we characterized bacteria from contaminated aquifers for their ability to utilize aromatic hydrocarbons under hypoxic (oxygen-limiting) conditions (initial dissolved oxygen concentration about 2 mg/l) with nitrate as an alternate electron acceptor. this is relevant to current intense efforts to establish favorable conditions for in situ bioremediation. using samples of granular activated carbon slurries from an operating groundwater treatment system, we isolated bacteria that are able to use ben ... | 1993 | 7764922 |
| purification and characterization of an alkaline lipase from pseudomonas fluorescens ak102. | an extracellular, novel alkaline lipase produced by pseudomonas fluorescens ak102 was purified by ultrafiltration, ammonium sulfate precipitation, and deae-toyopearl 650m and phenyl-toyopearl 650m column chromatographies. the purified enzyme was homogeneous on sds-page. the molecular weight was estimated to be about 33,000 by sds-page. the isoelectric point was ph 4.0 by isoelectric focusing. the ph stability was 4 to 10 and the optimum ph was 8 to 10. the optimum temperature was 55 degrees c an ... | 1994 | 7765474 |
| dna sequence of proline permease gene from pseudomonas fluorescens and predicted structure of proline permease. | the proline permease gene of pseudomonas fluorescens has been isolated from the promoter region isolated by using a promoter probe (transposon tn5-b21). by dna sequencing of 2222 bp the primary structure of permease (494 aa) was deduced. the dna sequence is 71.0% and 70.7% identical and the amino acid sequence is 75.8% and 76.0% similar to those of escherichia coli and salmonella typhimurium, respectively. | 1994 | 7765603 |
| the resistance of cellulases and xylanases to proteolytic inactivation. | the sensitivity of a range of cellulases and xylanases to proteolytic inactivation was investigated. the xylanases, all the clostridium thermocellum cellulases and cellulase e from pseudomonas fluorescens subsp. cellulosa exhibited no decrease in catalytic activity during a 3-h incubation with proteinases of the small intestine. under these conditions, the control escherichia coli enzymes analysed had half-lives of 4.3-13.5 min. the addition of substrate significantly decreased the sensitivity o ... | 1995 | 7766136 |
| d-glucosaminate aldolase activity of d-glucosaminate dehydratase from pseudomonas fluorescens and its requirement for mn2+ ion. | when d-glucosaminate dehydratase (gadh) was incubated with d-glucosaminate (glcna) in veronal buffer (vb; 0.01 m, ph 8.0), glcna was converted stoichiometrically to glyceraldehyde, pyruvate, and ammonia (aldolase reaction a). this reaction occurred in addition to the dehydratase reaction (conversion of glcna to 2-keto-3-deoxy-d-gluconate and ammonia: alpha,beta-elimination reaction, b). the ratio of the activities (a:b) was about 1:4. however, in potassium phosphate buffer (kpb; 0.04 m, ph 8.0), ... | 1995 | 7766176 |
| cloning of genes required for hypersensitivity and pathogenicity in pseudomonas syringae pv. aptata. | a genomic library of pseudomonas syringae pv. aptata strain ncppb 2664, which causes bacterial blight of sugar beet, lettuce and other plants, was constructed in the cosmid vector pcpp31. the 13.4 kb ecori fragment of the cosmid phir11, containing the hrp (hypersensitive response and pathogenicity) gene cluster of the closely related bacterium pseudomonas syringae pv. syringae strain 61, was used as a probe to identify a homologous hrp gene cluster in p. syringae pv. aptata. thirty of 2500 cosmi ... | 1995 | 7771767 |
| nucleotide sequence of the fad synthetase gene from corynebacterium ammoniagenes and its expression in escherichia coli. | the nucleotides of a bifunctional enzyme fad synthetase gene, which showed both flavokinase and atp:fmn adenylyltransferase activities, from corynebacterium ammoniagenes were sequenced. the fad synthetase gene product consisted of 338 amino acids and had a calculated molecular weight of 37,712. the deduced protein sequence of the fad synthetase shared a homology with those of the protein x of escherichia coli, which has been reported to have both flavokinase and atp:fmn adenylyltransferase activ ... | 1995 | 7772835 |
| [pseudomonas fluorescens: production of pyoverdine in human blood at 4 degrees c and cytotoxic effect of the pigment]. | pseudomonas fluorescens pab strain produced pyoverdine in a synthetic medium, this pigment was purified by solvent extraction and ion exchange, then sterilized by filtration. where cytotoxic effect on human leukocytes was assayed, death and lysis was observed. sublytic doses decreased leukocytes phagocytosis and chemotaxis. bacteria grew and produced pigment in blood stored a 4 degrees c, with a pyoverdine production of 0.13 mg/ml of serum after 5 days of incubation. | 1995 | 7784726 |
| cloning and sequence determination of the aspartase-encoding gene from brevibacterium flavum mj233. | a 2.5-kb ecori fragment containing the aspartase-encoding gene (aspa) of brevibacterium flavum mj233 was cloned into plasmid puc18 using southern hybridization with the escherichia coli aspa gene as a probe. the complete nucleotide (nt) sequence of the cloned dna indicated that the deduced gene product of the br. flavum aspa is composed of 526 amino acids (aa). comparison of the aa sequence to the corresponding sequences from e. coli, bacillus subtilis and pseudomonas fluorescens revealed 63, 47 ... | 1995 | 7789816 |
| (+)-(s)-dihydroaeruginoic acid, an inhibitor of septoria tritici and other phytopathogenic fungi and bacteria, produced by pseudomonas fluorescens. | three antibiotics were isolated from a ch2cl2 extract of the liquid culture of pseudomonas fluorescens strain pfm2. two of the antibiotics were identified as 2,4-diacetylphloroglucinol and pyoluterin. the structure elucidation, absolute stereochemistry, synthesis, and biological activities of the new antibiotic (+)-(s)- dihydroaeruginoic acid [1] are reported. | 1994 | 7798954 |
| growth temperature regulates the induction of beta-lactamase in pseudomonas fluorescens through modulation of the outer membrane permeation of a beta-lactam-inducing antibiotic. | the psychrotrophic bacterium, pseudomonas fluorescens strain mfo, is more sensitive to the beta-lactam mezlocillin at a low growth temperature (i.e. 8 degrees c) than at a higher growth temperature (28 degrees c). an early effect of this antibiotic at all temperatures is bacterial filamentation, but this occurs later at 8 degrees c than at 28 degrees c, which suggests a lower permeability of the cell envelopes to mezlocillin at low growth temperature. beta-lactamase production is later induced b ... | 1994 | 7812451 |
| [small peptides with antibiotic activity]. | peptide dkrfdle showed in vitro stable activity against gram negative bacteria i.e. escherichia coli and pseudomonas fluorescens when grown on the minimum glucose-mineral medium. substitutions of the n- and c-end amino acids in the molecule of the peptide decreased its antibiotic activity. comparison of the amino acid sequences of the known peptides with antibiotic activity made it possible to conclude that the presence of the doublet of the basic amino acids at the n-end and the doublet of the ... | 1994 | 7840703 |
| a plasmid responsible for malonate assimilation in pseudomonas fluorescens. | a novel, broad-host-range 60-kb r-plasmid, which encodes for malonate assimilation, was isolated from pseudomonas fluorescens and was designated ppsf1. pseudomonas, which can utilize malonate as a sole carbon source, was unable to grow on malonate medium upon curing with mitomycin c, indicating loss of plasmid ppsf1. furthermore, escherichia coli transformed with ppsf1 was able to grow on malonate medium as a sole carbon source. malonate decarboxylase, a key enzyme in malonate assimilation, was ... | 1994 | 7846146 |
| a non-modular endo-beta-1,4-mannanase from pseudomonas fluorescens subspecies cellulosa. | pseudomonas fluorescens subsp. cellulosa when cultured in the presence of carob galactomannan degraded the polysaccharide. to isolate gene(s) from p. fluorescens subsp. cellulosa encoding endo-beta-1,4-mannanase (mannanase) activity, a genomic library of pseudomonas dna, constructed in lambda zapii, was screened for mannanase-expressing clones using the dye-labelled substrate, azo-carob galactomannan. the nucleotide sequence of the pseudomonad insert from a mannanase-positive clone revealed a si ... | 1995 | 7848261 |
| malate and glucose in milk incubated with psychrotrophic bacteria. | l-malate consumption by the natural microflora of milk and a strain of pseudomonas fluorescens was followed during storage of milk at 7 degrees c. use of milk malate by somatic cells from mastitic milk was investigated and found to be insignificant. l-malate seems to be a potential indicator for the growth of psychrotrophic bacteria in refrigerated milk at levels between 10(5) and 10(7) cfu/ml. | 1994 | 7848783 |
| specificity of an esterase (xyld) from pseudomonas fluorescens subsp. cellulosa. | activity of an esterase from pseudomonas fluorescens subsp. cellulosa (xyld) on an insoluble feruloylated hemicellulose substrate (de-starched wheat bran) was dependent on the source of added endo-xylanase. the esterase exhibited high selectivity for the nature, position of linkage and size of the feruloylated oligosaccharides generated by hydrolysis of the hemicellulose. increased affinity of xyld with increasing size of the oligosaccharide substrate suggests that optimal activity is observed o ... | 1995 | 7873572 |
| structure of the catalytic core of the family f xylanase from pseudomonas fluorescens and identification of the xylopentaose-binding sites. | sequence alignment suggests that xylanases evolved from two ancestral proteins and therefore can be grouped into two families, designated f and g. family f enzymes show no sequence similarity with any known structure and their architecture is unknown. studies of an inactive enzyme-substrate complex will help to elucidate the structural basis of binding and catalysis in the family f xylanases. | 1994 | 7881909 |
| a numerical taxonomic study of fluorescent pseudomonas strains isolated from natural mineral waters. | forty-six strains of fluorescent pseudomonads, isolated from natural mineral waters, together with 12 strains from clinical material and 44 reference strains, were phenotypically classified by 281 characteristics. the data were processed by the dice similarity coefficient and unweighted pair group algorithm with arithmetic averages. eight clusters were defined at the 62% similarity level. clusters i, ii and iv were further divided into nine subclusters. virtually all the mineral water strains fa ... | 1995 | 7883648 |
| inhibition enzyme-linked immunosorbent assay for detection of pseudomonas fluorescens on meat surfaces. | an inhibition enzyme-linked immunosorbent assay was developed for pseudomonas fluorescens enumeration of meat surfaces. the assay detected contamination levels as low as 3 x 10(5) bacteria per ml and could be completed within 4 h. it could be used as a framework for a test system for quantifying p. fluorescens spoilage in meat products. | 1995 | 7887624 |
| [characteristics of pseudomonas fluorescens lipopolysaccharide]. | a lipopolysaccharide was isolated from pseudomonas fluorescens imv 1152 (biovar i). the fractions of the structural moieties of lipopolysaccharide macromolecule were extracted and studied separately. 3-hydroxydecanoic, 3-hydroxydodecanoic and 2-hydroxydodecanoic fatty acids were identified in lipid a, total quantity of those was more than 80%. in acid hydrolysate of lipid a the glucosamine and phosphoethanolamine were found. the rhamnose, arabinose, glucose, glucosamine and phosphorus were ident ... | 1994 | 7898395 |
| characterization of the pcp gene of pseudomonas fluorescens and of its product, pyrrolidone carboxyl peptidase (pcp). | the gene pcp, encoding pyrrolidone carboxyl peptidase (pcp), from pseudomonas fluorescens mfo was cloned and its nucleotide sequence was determined. this sequence contains a unique open reading frame (pcp) coding for a polypeptide of 213 amino acids (m(r) 22,441) which has significant homology to the pcps from streptococcus pyogenes, bacillus subtilis, and bacillus amyloliquefaciens. comparison of the four pcp sequences revealed two highly conserved motifs which may be involved in the active sit ... | 1994 | 7909543 |
| production of a mosquitocidal exotoxin by a pseudomonas fluorescens strain. | | 1994 | 7914905 |
| survival and respiratory activity of genetically engineered pseudomonas spp. exposed to antimicrobial agents in broth and soil. | the effectiveness of seven chemical disinfectants were tested against genetically engineered pseudomonas spp. under optimal growth conditions. each chemical was tested to determine how quickly 10(8) cells/ml pseudomonas fluorescens c5t (containing bacillus thuringiensis endotoxin gene) were killed in king's b broth at 30 degrees c. the minimal bactericidal concentrations (mbc) for calcium hypochlorite, benzalkonium chloride, germiphene and spectrum clear bath were 0.06% (w/v), 0.01% (w/v), 0.08% ... | 1994 | 7921352 |
| purification and properties of zinc-metallophospholipase c from pseudomonas fluorescens. | phospholipase c produced by pseudomonas fluorescens, isolated as a laboratory contaminant, has been purified to apparent homogeneity by ammonium sulphate fractionation, anion-exchange and size-exclusion chromatographies. the apparent molecular mass of the purified polypeptide was 39.5 kda. purified preparations of phospholipase c were used to characterize its enzymic properties and to obtain amino acid sequence of the n-terminus of the molecule. the p. fluorescens phospholipase c hydrolysed ptde ... | 1994 | 7925409 |
| purification and characterization of a ferulic acid decarboxylase from pseudomonas fluorescens. | a ferulic acid decarboxylase enzyme which catalyzes the decarboxylation of ferulic acid to 4-hydroxy-3-methoxystyrene was purified from pseudomonas fluorescens ui 670. the enzyme requires no cofactors and contains no prosthetic groups. gel filtration estimated an apparent molecular mass of 40.4 (+/- 6%) kda, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a molecular mass of 20.4 kda, indicating that ferulic acid decarboxylase is a homodimer in solution. the purified enz ... | 1994 | 7928951 |
| relationship of protein structure of isoleucyl-trna synthetase with pseudomonic acid resistance of escherichia coli. a proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-trna synthetase. | to elucidate the mode of action of pseudomonic acid, we have compared the deduced amino acid sequences of isoleucyl-trna synthetases (ilers) from wild-type escherichia coli strain mc4100, a pseudomonic acid-resistant mutant (strain ps102) of mc4100, and a pseudomonic acid-producing strain, pseudomonas fluorescens. compared with the wild-type enzyme, the deduced amino acid sequence of e. coli mutant iles gene in strain ps102 shows a single amino acid substitution of leucine for phenylalanine at r ... | 1994 | 7929087 |
| selective cysteine-->serine replacements in p-hydroxybenzoate hydroxylase from pseudomonas fluorescens allow the unambiguous assignment of cys211 as the site of modification by spin-labeled p-chloromercuribenzoate. | p-hydroxybenzoate hydroxylase from pseudomonas fluorescens contains five sulfhydryl groups per subunit. cysteine-->serine replacements show that the thiols are not essential for catalysis. the increased dissociation constant for fad in mutant cys158ser suggests that cys158 is important for the solvation of the pyrophosphate moiety of the prosthetic group. wild-type p-hydroxybenzoate hydroxylase is rapidly inactivated by mercurial compounds. inactivation by a spin-labeled derivative of p-chlorome ... | 1994 | 7937711 |
| phenotypic and genotypic diversity of fluorescent pseudomonads isolated from field-grown sugar beet. | a sample of 30 fluorescent pseudomonads isolated from the phyllosphere of sugar beet throughout a single growing season and shown to be closely related on the basis of fatty acid methyl ester (fame) analysis was subjected to detailed phenotypic and genotypic characterization. phenotypic traits were assessed on the basis of biochemical properties, assimilation of sole carbon sources, fame analysis, organic pyrolysate content (ms-pyrolysis), and total cellular protein profiles. with the exception ... | 1994 | 7952185 |
| fluorescent pseudomonas species categorized by using polymerase chain reaction (pcr)/restriction fragment analysis of 16s rdna. | a rapid procedure for the identification of fluorescent pseudomonads, based on the polymerase chain reaction (pcr) and restriction fragment analysis of 16s rdna genes is described. thirty-one strains belonging to 10 different pseudomonas species of the pseudomonas fluorescens rrna branch were characterized. amplified rdna was digested with 13 different restriction endonucleases. the combined data from restriction analysis enabled the definition of 17 different 16s rdna genotypes. all type strain ... | 1994 | 7952328 |
| lipase and acidic phosphatase from the psychrotrophic bacterium pseudomonas fluorescens: two enzymes whose synthesis is regulated by the growth temperature. | in the psychrotrophic bacterium pseudomonas fluorescens, the production of several enzymes that otherwise differ in their cell location, growth phase production and inducibility appeared to be similarly regulated by the growth temperature. in order to determine the level of this regulation, the expression of the apo and lip genes encoding two of these enzymes, the acidic phosphatase and lipase, respectively, was studied at different temperatures. both genes were optimally expressed at 17.5 degre ... | 1994 | 7958764 |
| sequence of the coba gene encoding s-adenosyl-l-methionine: uroporhyrinogen iii methyltransferase of pseudomonas fluorescens. | sequence analysis of the region downstream of oprf, from pseudomonas fluorescens oe 28.3, revealed the presence of coba homologue encoding a putative s-adenosyl-l-methionine: uroporhyrinogen iii methyltransferase. a similar gene organization exists in p. aeruginosa. | 1994 | 7959054 |
| molecular characterization of the extracellular poly(3-hydroxyoctanoic acid) [p(3ho)] depolymerase gene of pseudomonas fluorescens gk13 and of its gene product. | phazpfi, the gene encoding the extracellular poly(3-hydroxyoctanoic acid) depolymerase of pseudomonas fluorescens gk13, was cloned, sequenced, and characterized. it comprises 837 bp and is transcribed as a monocistronic message of about 950 bp from a putative sigma 70-like promoter 32 bp upstream of the atg start codon. the deduced protein of 278 amino acids reveals a typical leader peptide at its n terminus. when expressed in escherichia coli, the mature depolymerase started with ala-23, wherea ... | 1994 | 7961472 |
| [pyoverdin from pseudomonas fluorescens: binding to the globulin fraction of serum and its effect on serological reaction titers]. | pseudomonas fluorescens grows and produces pigment in refrigerated human blood at 4 degrees c. saline precipitation of plasma showed that globulin and albumin fractions retained pyoverdin at different concentrations. by dialysis it was possible to determine that the pigment attached or aggregated to the protein in total plasma as well as in the fraction obtained by saline precipitation. a greater binding was observed at the globulin fraction. gamma-globulin immunological activity was reduced due ... | 1994 | 7973183 |
| a novel, small endoglucanase gene, egl5, from trichoderma reesei isolated by expression in yeast. | a method is presented for the isolation of genes encoding hydrolytic enzymes without any knowledge of the corresponding proteins. cdna made from the organism of interest is cloned into a yeast vector to construct an expression library in the yeast saccharomyces cerevisiae. colonies producing hydrolytic enzymes are screened by activity plate assays. in this work, we constructed a yeast expression library from the filamentous fungus trichoderma reesei and isolated a new beta-1,4-endoglucanase gene ... | 1994 | 7984103 |
| enzyme-catalysed kinetic resolution of 4-endo-hydroxy-2-oxabicyclo[3.3.0]oct-7-en-3-one and employment of the pure enantiomers for the synthesis of anti-viral and hypocholestemic agents. | the endo-hydroxylactone (+/-)-(1) was resolved by enantioselective acetylation using candida cylindracea lipase or preferentially pseudomonas fluorescens lipase (pfl). alternatively the corresponding butyrate (+/-)-(3) was hydrolysed with pfl to give the ester (+)-(1s,4r,5s)-(3) and the alcohol (-)-(1r,4s,5r)-(1). the latter compound was converted into carbovir (-)-(1r,4s)-(12) while the ester (+)-(3) was transformed into the delta-lactone (+)-(3r,5s)-(18). the exo-hydroxylactone (+/-)-(2) was r ... | 1994 | 8000858 |
| ethylbenzene degradation by pseudomonas fluorescens strain ca-4. | pseudomonas fluorescens strain ca-4 is a bioreactor isolate capable of ethylbenzene degradation. transposon mutagenesis and enzyme assays have been performed which allow us to propose the ethylbenzene degradative pathway in operation in this strain. ethylbenzene is initially converted to 2-phenylethanol. this is degraded to phenylacetaldehyde and then to phenylacetic acid. the major inducer of the pathway is ethylbenzene itself. the pathway is regulated by the presence of non-aromatic carbon sou ... | 1994 | 8001765 |
| genetic evidence that the gaca gene encodes the cognate response regulator for the lema sensor in pseudomonas syringae. | mutational analysis of the bean-pathogenic pseudomonas syringae pv. syringae strain b728a has led to the genetic identification of the gaca gene as encoding the response regulator for the unlinked lema sensor kinase. the analysis of a collection of spontaneous mutants of p. syringae pv. syringae suggested that the gaca gene was involved in lesion formation and the production of protease and syringomycin. the gaca gene originally was identified as a regulator of extracellular antibiotic productio ... | 1994 | 8002569 |
| luminescence-based detection of activity of starved and viable but nonculturable bacteria. | a naturally luminescent bacterium, vibrio harveyi, and two bacteria, escherichia coli and pseudomonas fluorescens, which had been genetically marked with luminescence were starved in liquid medium at 4 and 30 degrees c for 54 days. total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively, and population activity was measured by luminometry. v. harveyi became noncultur ... | 1994 | 8017919 |
| optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium. | an optical whole-cell biosensor based on a genetically engineered bioluminescent catabolic reporter bacterium was developed for continuous on-line monitoring of naphthalene and salicylate bioavailability and microbial catabolic activity potential in waste streams. the bioluminescent reporter bacterium, pseudomonas fluorescens hk44, carries a transcriptional nahg-luxcdabe fusion for naphthalene and salicylate catabolism. exposure to either compound resulted in inducible bioluminescence. the repor ... | 1994 | 8017932 |