| environmental sampling to detect burkholderia cepacia in a cystic fibrosis outpatient clinic. | | 1996 | 8839652 |
| microbial pathogenesis in cystic fibrosis: mucoid pseudomonas aeruginosa and burkholderia cepacia. | respiratory infections with pseudomonas aeruginosa and burkholderia cepacia play a major role in the pathogenesis of cystic fibrosis (cf). this review summarizes the latest advances in understanding host-pathogen interactions in cf with an emphasis on the role and control of conversion to mucoidy in p. aeruginosa, a phenomenon epitomizing the adaptation of this opportunistic pathogen to the chronic chourse of infection in cf, and on the innate resistance to antibiotics of b. cepacia, person-to-p ... | 1996 | 8840786 |
| investigation of nosocomial respiratory infection due to pseudomonas cepacia by arbitrarily primed polymerase chain reaction. | we used dna fingerprinting by the arbitrarily primed polymerase chain reaction (ap-pcr) technique for an epidemiologic investigation of pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. this approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (pfge). the patterns of gel electrophoresis of the products of ap-pcr differed significantly according to differences in the concentration of mg2+ and in ph. ap-pcr and pfge was ... | 1995 | 8849650 |
| [utility of pyrrolidonyl-arylamidase detection for typing enterobacteriaceae and non-fermenting gram-negative bacteria]. | detection of pyrrolidonyl-aryl-amidase activity (pyr) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. however, only few studies evaluating its usefulness with gram-negative rods have been published. thus, a prospective study including 542 and 215 unique clinical isolates of enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. strains were identified by conventional methods. pyr ... | 1995 | 8850133 |
| susceptibilities of non-pseudomonas aeruginosa gram-negative nonfermentative rods to ciprofloxacin, ofloxacin, levofloxacin, d-ofloxacin, sparfloxacin, ceftazidime, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, and imipenem. | agar dilution mics of 10 agents against 410 non-pseudomonas aeruginosa gram-negative nonfermentative rods were determined. mics at which 50 and 90% of the isolates were inhibited, respectively, were as follows (in micrograms per milliliter): sparfloxacin, 0.5 and 8.0; levofloxacin, 1.0 and 8.0; ciprofloxacin, 2.0 and 32.0; ofloxacin, 2.0 and 32.0; d-ofloxacin, 32.0 and > 64.0; ceftazidime, 8.0 and 64.0; piperacillin with or without tazobactam, 16.0 and > 64.0; trimethoprim-sulfamethoxazole, 0.5 ... | 1996 | 8851609 |
| molecular analysis of burkholderia (pseudomonas) cepacia: differentiation between relapse and reinfection in a case of recurrent bacteremia. | | 1996 | 8852993 |
| burkholderia (pseudomonas) cepacia and cystic fibrosis: the epidemiology in belgium. | burkholderia cepacia has become an increasingly recognized pathogen among cystic fibrosis (cf) patients and its potential role in declining pulmonary function or unexpected fatal outcome has caused widespread concern. direct person-to-person transmission has been documented and a segregation policy of cf patients colonized with b.cepacia from non-colonized cf patients is widely adopted. since this policy has a dramatic impact on social behaviour of cf patients it is imperative that clinical labo ... | 1996 | 8858887 |
| distribution and transmission of pseudomonas aeruginosa and burkholderia cepacia in a hospital ward. | genotyping and antibiotic susceptibility testing were used to analyze pseudomonas aeruginosa and burkholderia cepacia strains from sink drain from 14 pediatric patients with cystic fibrosis (cf) and from hospital personnel as part of a 4 week prospective study of strain transmission in a pediatric ward. a total of 87.5% of all washbasin drains were contaminated with p. aeruginosa [10(2) to 10(5) colony forming units (cfu)/ml sink fluid], whereas b. cepacia was found only once in a sink drain. fr ... | 1996 | 8882212 |
| a genetic analysis system of burkholderia cepacia: construction of mobilizable transposons and a cloning vector. | a genetic analysis system of burkholderia cepacia (bc) was developed which included transposon mutagenesis and complementation of mutation with the cloned genes of interest. to deliver the transposon in this multidrug-resistant microorganism, two plasmids, pkn30 and pkn31, were constructed which contained tn5 derivatives, tn5-30tp and tn5-31tp, respectively, carrying kmr and tpr genes. the plasmids have the origin of cole1 replication and the mobilization gene of rp4. tn5-31tp was mobilized to b ... | 1996 | 8890733 |
| genomic complexity and plasticity of burkholderia cepacia. | burkholderia cepacia has attracted attention because of its extraordinary degradative abilities and its potential as a pathogen for plants and for humans. this bacterium was formerly considered to belong to the genus pseudomonas in the gamma-subclass of the proteobacteria, but recently has been assigned to the beta-subclass is based on rrn gene sequence analyses and other key phenotypic characteristics. the b. cepacia genome is comprised of multiple chromosomes and is rich in insertion sequences ... | 1996 | 8900054 |
| identification and sequencing of a novel insertion sequence, is1471, in burkholderia cepacia strain 2a. | a novel insertion sequence (is), is1471, has been identified which is inserted into is element is1071 possessed by plasmid pijb1 in burkholderia cepacia strain 2a. nucleotide sequencing has revealed that is1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp duplication of the target sequence is1471 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39406 da. searches of dna and protein sequence da ... | 1996 | 8900064 |
| microbial contamination of antiseptics and disinfectants. | there have been a number of reports on microbial contamination of antiseptics and disinfectants. at present, however, the necessity of measures to prevent contamination do not seem to be fully appreciated. we investigated microbial contamination of antiseptics and disinfectants that are used in our hospital. | 1996 | 8902114 |
| bone graft contamination from a water de-ionizer during processing in a bone bank. | an organism resembling burkholderia (formerly pseudomonas) cepacia was isolated from cultures taken during processing of six bone grafts. the source of the contamination was traced to a water de-ionizer; the problem had begun when procedures had been changed, so that water from the de-ionizer had been used without sterilization. cultures of bone for grafting not only protect recipients from infection, but act also as useful controls for processes involved in bone banking. | 1996 | 8904374 |
| home-use nebulizers: a potential primary source of burkholderia cepacia and other colistin-resistant, gram-negative bacteria in patients with cystic fibrosis. | inhalation of aerosols contaminated with gram-negative bacteria generated from home-use nebulizers used by cystic fibrosis (cf) patients may be a primary route for bacterial colonization of the lung. burkholderia cepacia was isolated from 3 of [corrected] 35 home-use nebulizers, and stenotrophomonas maltophilia was isolated from 4 of 35 home-use nebulizers. sputum cultures for two patients whose nebulizers were contaminated with b. cepacia did not yield the organism. however, dna macrorestrictio ... | 1996 | 8904419 |
| mechanism for synergism between sulphonamides and trimethoprim clarified. | pseudomonas aeruginosa, escherichia coli, pseudomonas cepacia and moraxella catarrhalis were selected for their markedly different resistance patterns to sulphonamides and trimethoprim. in addition, strains of e. coli and p. cepacia were selected having different resistance profiles to the inhibition of dihydropteroate synthetase and dihydrofolate reductase. all inhibitors of dihydropteroate synthetase combined in any combination with inhibitors of dihydrofolate reductase resulted in mutual enha ... | 1996 | 8910868 |
| bronchiolitis obliterans organizing pneumonia (boop) in lung transplant recipients. | we reviewed all tissue specimens from 163 transplant patients (108 double lung transplant [dlt], 55 single lung transplant [slt]) between november 1983 and january 1994 for abnormalities indicating bronchiolitis obliterans organizing pneumonia (boop) and found 17 cases (14 dlt and 3 slt). of the three slts, boop was diagnosed by open lung biopsy (olb) in two and one was found at autopsy. of the 14 dlts, boop was diagnosed by transbronchial biopsy (tbb) specimens (9), olb specimens (2), autopsy ( ... | 1996 | 8915212 |
| trichloroethylene degradation and mineralization by pseudomonads and methylosinus trichosporium ob3b. | to examine the trichloroethylene (c2hcl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of c2hcl3 mineralization were evaluated for pseudomonas cepacia g4, pseudomonas cepacia g4 pr1, pseudomonas mendocina kr1, pseudomonas putida f1, and methylosinus trichosporium ob3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for c2hcl3 degradation. by varying the c2hcl3 concentration from 5 microm to 75 microm, vmax ... | 1996 | 8920197 |
| invasion of respiratory epithelial cells by burkholderia (pseudomonas) cepacia. | pulmonary infections caused by burkholderia (pseudomonas) cepacia are an important cause of morbidity and mortality in cystic fibrosis (cf) patients. several features suggestive of cellular invasion and intracellular sequestration of b. cepacia in cf are persistence of infection in the face of antibiotic therapy to which the organism demonstrates in vitro susceptibility and a propensity to cause bacteremic infections in patients with cf. epithelial cell invasion was demonstrated in vitro in a549 ... | 1996 | 8926068 |
| the dsbb gene product is required for protease production by burkholderia cepacia. | burkholderia cepacia kf1, isolated from a pneumonia patient, produces a 37-kda extracellular metalloprotease. a protease-deficient and lipase-proficient mutant, kft1007, was complemented by a clone having an open reading frame coding for a 170-amino-acid polypeptide which showed significant homology to escherichia coli dsbb. kft1007, a presumed dsbb mutant, also failed to show motility, and both protease secretion and motility were restored by the introduction of the cloned dsbb gene of b. cepac ... | 1996 | 8926116 |
| nosocomial burkholderia cepacia outbreaks and pseudo-outbreaks. | | 1996 | 8934237 |
| three-year outbreak of pseudobacteremia with burkholderia cepacia traced to a contaminated blood gas analyzer. | between november 1990 and june 1993, burkholderia cepacia was isolated from the blood cultures of 13 neonates born at the ottawa general hospital. eight of the 13 neonates appeared symptomatic, and only 4 were treated with appropriate antimicrobial therapy, but all improved clinically. in august 1993, the blood gas analyzer in the neonatal intensive-care unit was found to be contaminated heavily with b cepacia. eight available patient isolates were identical to the isolates recovered from the bl ... | 1996 | 8934241 |
| an outbreak of burkholderia cepacia lower respiratory tract infection associated with contaminated albuterol nebulization solution. | an outbreak of burkholderia cepacia lower respiratory tract colonization and infection occurred in the adult intensive-care units in various geographic locations throughout our hospital. forty-four patients became colonized or infected over an 11-month period, whereas b cepacia had been isolated from only 13 patients in the preceding 48 months. environmental cultures revealed the source to be extrinsically contaminated albuterol nebulization solution. polymerase chain reaction-ribotyping confirm ... | 1996 | 8934242 |
| epidemiology of burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic dna fingerprinting. | we fingerprinted a collection of 627 burkholderia cepacia isolates from 255 patients with cystic fibrosis (cf) and 43 patients without cf and from the environment, by a pcr-based randomly amplified polymorphic dna (rapd) method with primers selected for their ability to produce discriminatory polymorphisms. the rapd typing method was found to be reproducible and discriminatory, more sensitive than pcr ribotyping, and able to group epidemiologically related b. cepacia strains previously typed by ... | 1996 | 8940422 |
| risk of microbial contamination with multiple use of 5-fluorouracil vials. | to determine whether microorganisms are able to survive in a solution of 50 mg/ml of 5-fluorouracil (5-fu) and, therefore, whether there is a risk of vial contamination with multiple use. | 1996 | 8946292 |
| epidemiology of pulmonary colonization with burkholderia cepacia in cystic fibrosis patients. the french observatoire burkholderia cepacia study group. | | 1996 | 8950569 |
| biodegradation of 2,4,5-trichlorophenoxyacetic acid by burkholderia cepacia strain ac1100: evolutionary insight. | many microorganisms in nature have evolved new genes which encode catabolic enzymes specific for chlorinated aromatic substrates, allowing them to utilize these compounds as sole sources of carbon and energy. an understanding of the evolutionary mechanisms involved in the acquisition of such genes may facilitate the development of microorganisms with enhanced capabilities of degrading highly chlorinated recalcitrant compounds. a number of studies have been based on microorganisms isolated from t ... | 1996 | 8955624 |
| evaluation of contamination of the dentist and dental surgery environment with burkholderia (pseudomonas) cepacia during treatment of children with cystic fibrosis. | pulmonary colonization with burkholderia (pseudomonas) cepacia in cystic fibrosis (cf) patients has been associated with high morbidity and mortality. the aim of this study was to investigate whether the dentist and the dental surgery environment become contaminated during treatment of cf patients colonized with b. cepacia. extensive environmental sampling was carried out during aerosol-generating treatment sessions on four cf patients whose sputum was persistently colonized with b. cepacia. sam ... | 1995 | 8957838 |
| burkholderia cepacia: medical, taxonomic and ecological issues. | the increasing challenge posed by multiresistant saprophytes in medical microbiology is strikingly demonstrated by the emergence of burkholderia (formerly pseudomonas) cepacia as an opportunist pathogen in immunocompromised patients, particularly individuals with chronic granulomatous disease and cystic fibrosis (cf). best known previously as a phytopathogen and the cause of soft rot of onions, b. cepacia presents three major problems for the cf community: innate multiresistance to antimicrobial ... | 1996 | 8958242 |
| rp4::mu3a-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway. | chromosomal dna fragments encoding the ability to utilize biphenyl as sole carbon source (bph+) were mobilized by means of plasmid rp4::mu3a from strain jb1 (tentatively identified as burkholderia sp.) to alcaligenes eutrophus ch34 at a frequency of 10(-3) per transferred plasmid. the mobilized dna integrated into the recipient chromosome or was recovered as catabolic prime plasmids. three bph+ prime plasmids were transferred from a. eutrophus to escherichia coli and back to a. eutrophus without ... | 1996 | 8969525 |
| comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. | the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ... | 1996 | 8975612 |
| succession and convergence of biofilm communities in fixed-film reactors treating aromatic hydrocarbons in groundwater. | community composition, succession, and performance were compared in three fluidized bed reactors (fbr) operated to test preemptive colonization and the influence of toluene compared with a mixture of benzene, toluene, and p-xylene (btx) as feeds. one reactor was inoculated with toluene-degrading strains pseudomonas putida paw1, burkholderia cepacia g4, and b. pickettii pko1. paw1 outcompeted the other two strains. when groundwater strains were allowed to challenge the steady-state biofilm develo ... | 1997 | 8979355 |
| trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation. | trichloroethylene is oxidized by several types of nonspecific bacterial oxygenases. toluene 2-monooxygenase from burkholderia cepacia g4 is implicated in trichloroethylene oxidation and is uniquely suggested to be resistant to turnover-dependent inactivation in vivo. in this work, the oxidation of trichloroethylene was studied with purified toluene 2-monooxygenase. all three purified toluene 2-monooxygenase protein components and nadh were required to reconstitute full trichloroethylene oxidatio ... | 1997 | 8981984 |
| systematic study of the genus vogesella gen. nov. and its type species, vogesella indigofera comb. nov. | a blue-pigmented colony that had a metallic copper-colored sheen was isolated in 1973 from a standard spread plate count preparation of oxidation pond sediment. over the next 11 years, an additional 12 strains of blue-pigmented bacteria were isolated from freshwater samples and compared to several reference strains of bacteria. morphological and biochemical tests revealed that these 13 isolates were very similar to [pseudomonas] indigofera atcc 19706t (t = type strain) and atcc 14036. a numerica ... | 1997 | 8995797 |
| molecular epidemiology of burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans in a cystic fibrosis center. | burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans have been isolated with increasing frequency from the sputum of patients with cystic fibrosis in a pediatric hospital. in 1994-95, 27 of 120 patients were persistently colonized, 17 with burkholderia cepacia, eight with alcaligenes xylosoxidans, and five with stenotrophomonas maltophilia. genotyping of 220 clinical isolates revealed that most of the burkholderia cepacia strains were clonally related, suggesting eith ... | 1996 | 8997562 |
| structure of the o-specific polysaccharide from burkholderia vietnamiensis strain lmg 6998. | the putative o-specific polymer containing d-mannose and l-rhamnose was isolated from the lipopolysaccharide obtained from cells walls of burkholderia vietnamiensis strain lmg 6998. nmr and degradative studies showed that the polymer has a linear trisaccharide repeating-unit of the structure shown. the same polymer carrying an o-acetyl group at position 3 of the 4-substituted mannose residue has previously been found as the o antigen in the related species burkholderia cepacia serogroup j. [form ... | 1996 | 9002192 |
| identification of neutralizing epitopes on pseudomonas aeruginosa elastase and effects of cross-reactions on other thermolysin-like proteases. | monoclonal antibodies (mabs) to a burkholderia (pseudomonas) cepacia 36-kda protease (pscp) which neutralize pscp and pseudomonas aeruginosa elastase but not p. aeruginosa alkaline protease have been isolated (c. kooi et al., infect. immun. 62:2811-2817, 1994). these mabs, designated 36-6-6 and 36-6-8, react with n-chlorosuccinimide cleavage products of p. aeruginosa elastase, consistent with the recognition of a 13.9-kda fragment which contains the active site. overlapping 9-mer peptides that s ... | 1997 | 9009299 |
| induction of biologically active interleukin-8 from lung epithelial cells by burkholderia (pseudomonas) cepacia products. | the frequency of isolation of burkholderia cepacia from the sputum of cystic fibrosis (cf) patients is increasing. using the human a549 lung epithelial cell line, we have investigated the ability of b. cepacia exoproducts to stimulate interleukin-8 (il-8) release. cell-free supernatants from a panel of cf clinical, non-cf clinical, and nonclinical b. cepacia isolates were found to stimulate il-8 release, with levels ranging from 11.8 +/- 2.8 to 80.0 +/- 3.5 ng/ml. a similar pattern was seen at t ... | 1997 | 9009321 |
| structural studies of the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia, isolated from cystic fibrosis. | the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. based on the sugar composition and permethylation analyses, supported by glc-ms and nmr spectroscopy analyses, the repeating-unit of the polysaccharide was established as -->3)-beta-d-glcp-(1-->3)-[4,6-o-(1-carboxyethylidene)]-alpha-d-gal p-(1-->. | 1996 | 9011377 |
| polymerase chain reaction for the detection of pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia in sputum of patients with cystic fibrosis. | occurrence of pseudomonas aeruginosa, stenotrophomonas (xanthomonas) maltophilia and burkholderia (pseudomonas) cepacia in sputum of cystic fibrosis (cf) patients was demonstrated with a simple and rapid polymerase chain reaction (pcr) technique. the pcr was performed with a set of three primer pairs based on 16s rrna sequences after sputum preparation with dithiothreitol and naoh lysis. all three pathogens could be individually detected by the use of this technique. to prevent carry-over contam ... | 1996 | 9025076 |
| identifying treatments that halt progression of pulmonary disease in cystic fibrosis. | rapid progress in cystic fibrosis research affords the possibility of halting the progress of the lung disease. we used data from 215 patients who had sputum cultures negative for burkholderia cepacia, at least one outpatient pulmonary function test during 1990, and at least one test a year later to estimate the number of subjects and study duration required to demonstrate that a hypothetical treatment reduces the rate of decline of forced expiratory volume in 1 s (fev1) to zero. mean rate of de ... | 1997 | 9029632 |
| multifactorial pathogenic mechanisms of burkholderia pseudomallei as suggested from comparison with burkholderia cepacia. | with the purpose to elucidate the pathogenesis of disease due to burkholderia pseudomallei some biological and biochemical properties of this species were studied in comparison with b. cepacia, since the difference in the level of virulence between the two species is remarkable despite of their toxonomic closeness. b. pseudomallei was distinct from b. cepacia in the capability to grow under anaerobic conditions, with positive nitrate respiration, excretion of high-molecular polysaccharides into ... | 1996 | 9031412 |
| the crystal structure of a triacylglycerol lipase from pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. | . lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. true lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of ena ... | 1997 | 9032073 |
| the open conformation of a pseudomonas lipase. | . the interfacial activation of lipases results primarily from conformational changes in the enzymes which expose the active site and provide a hydrophobic surface for interaction with the lipid substrate. comparison of the crystallization conditions used and the structures observed for a variety of lipases suggests that the enzyme conformation is dependent on solution conditions. pseudomonas cepacia lipase (pcl) was crystallized in conditions from which the open, active conformation of the enzy ... | 1997 | 9032074 |
| identification of burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. | we evaluated 819 isolates referred to us as "burkholderia cepacia" from cystic fibrosis (cf) clinics and research laboratories from five countries; 28 (3.4%) were not b. cepacia. a further 12 (1.5%) organisms appeared to be other burkholderia species, but identification could not be confirmed by conventional means. the most prevalently misidentified organisms were stenotrophomonas maltophilia, alcaligenes xylosoxidans, and comamonas acidovorans. many of these organisms grew on oxidation-fermenta ... | 1997 | 9041399 |
| [microbiological parameters of clinical interest in pulmonary infection in cystic fibrosis]. | a 5-year study in patients with cystic fibrosis was carried out in order to gain a better understanding of the microbiological factors influencing clinical status and evolution. | 1996 | 9044640 |
| cloning and expression of the major porin protein gene opcp of burkholderia (formerly pseudomonas) cepacia in escherichia coli. | the outer membrane protein opcp1 of burkholderia (formerly pseudomonas) cepacia is one of the subunits forming the porin oligomer opcpo by non-covalent association. opcp1 was cleaved with lysyl endopeptidase and the n-terminal amino acid (aa) sequences of the polypeptide fragments were determined. based on the sequence information, we cloned the opcp gene on a 10-kb ecori dna fragment of the b. cepacia atcc25416 chromosome. nucleotide (nt) sequencing revealed a 1086-bp open reading frame (orf), ... | 1997 | 9047353 |
| probing of pseudomonas aeruginosa, pseudomonas aureofaciens, burkholderia (pseudomonas) cepacia, pseudomonas fluorescens, and pseudomonas putida with the ferripyochelin receptor a gene and the synthesis of pyochelin in pseudomonas aureofaciens, pseudomonas fluorescens, and pseudomonas putida. | the ferripyochelin receptor a (fpta) gene codes for the transport of the ferrisiderophore ferripyochelin in pseudomonas aeruginosa. a p. aeruginosa fpta internal fragment was used to probe chromosomal dna from p. aureofaciens, b. cepacia, p. fluorescens, p. putida, and five strains of p.aeruginosa. these bacteria all contained dna that hybridized to the fpta fragment. four of the five p. aeruginosa strains displayed marked and identical patterns, indicating a high degree of sequence similarities ... | 1997 | 9058547 |
| serum igg response to an outer membrane porin protein of burkholderia cepacia in patients with cystic fibrosis. | early and accurate diagnosis of burkholderia cepacia infection is important, particularly if segregation is to prevent patient-to-patient transmission. we have examined the serum response to a b. cepacia-specific 80-kda outer membrane protein. 21 patients colonised with b. cepacia and pseudomonas aeruginosa for 2-51 months (mean 11 months) were age- and sex-matched with 21 patients colonised with p. aeruginosa but not b. cepacia. the 80-kda protein was recovered by electroelution from outer memb ... | 1997 | 9061354 |
| isolation from clinical sources of burkholderia cepacia possessing characteristics of burkholderia gladioli. | further characterization of 32 clinical isolates originally identified as burkholderia cepacia by biochemical and fatty acid profiling revealed the presence of 12 strains bearing partial resemblance to the closely related species burkholderia gladioli. these strains were highly resistant to a wide range of antibiotics including ticarcillin (with or without clavulanic acid), cefsulodin, imipenem, the aminoglycosides, colistin and fosfomycin. they typically produced a red-brown pigment and possess ... | 1997 | 9069537 |
| substituted amines synergistic with tobramycin against burkholderia cepacia in vitro. | antibiotic-resistant burkholderia cepacia is an important pathogen in cystic fibrosis. we have previously shown enhanced tobramycin susceptibility of most strains in the presence of the drug amiloride. others have shown similar findings in the presence of cationic agents with amine groups. to examine the relationship of substituted amine drug structure and synergy potential with tobramycin, the in vitro activity of 45 non-antibiotic substituted amine and related compounds in the presence and abs ... | 1997 | 9084918 |
| exercise testing and prognosis in adult cystic fibrosis. | the assessment of prognosis is an important issue in cystic fibrosis. the prognostic value of exercise testing in comparison with other predictors of mortality was examined. | 1997 | 9093351 |
| a fusion promoter created by a new insertion sequence, is1490, activates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in burkholderia cepacia ac1100. | transposition and transcriptional activation by insertion sequences in burkholderia cepacia ac1100 were investigated. two closely related new elements, is1413 and is1490, were identified and characterized. these elements are not highly related to other insertion sequences identified in ac1100 or other b. cepacia isolates. based on their structures and the sequences of the inverted terminal repeats and the putative transposase protein, the insertion elements (is elements) are similar to ist2 of t ... | 1997 | 9098071 |
| enhanced host defense after gene transfer in the murine p47phox-deficient model of chronic granulomatous disease. | the p47phox-/- mouse exhibits a phenotype similar to that of human chronic granulomatous disease (cgd) and, thus, is an excellent model for the study of gene transfer technology. using the moloney murine leukemia virus-based retroviral vector mfg-s encoding the human form of p47phox, we performed ex vivo gene transfer into sca-1+ p47phox-/- marrow progenitor cells without conditioning of donors with 5-fluorouracil. transduced progenitors were transplanted into moderately irradiated (500 cgy), g- ... | 1997 | 9116268 |
| pcr ribotyping and endonuclease subtyping in the epidemiology of burkholderia cepacia infection. | because of conflicting data about hospital-based transmission of burkholderia (pseudomonas) cepacia, an important respiratory pathogen in cystic fibrosis (cf), we compared strains found in sputum, lung, or blood of 29 cf patients in our center from 1988 to 1994, studying the relationship between strain and hospital exposure of incident and that of prevalent cases. exposure was defined as a concurrent hospital stay between a prevalent and an incident case. b. cepacia strains were determined by po ... | 1997 | 9117036 |
| comparative in vitro activity of meropenem versus other extended-spectrum antimicrobials against randomly chosen and selected resistant clinical isolates tested in 26 north american centers. | the in vitro antibacterial activity of meropenem and up to nine other antimicrobials was compared in studies at 26 north american centers from 1989 to 1992 with use of standardized and controlled procedures for determining minimal inhibitory concentrations (mics) against 12,483 recent clinical isolates and additional drug-resistant strains. overall, carbapenems were the most active drugs. the antibacterial activity of meropenem was consistent against random isolates in all centers; however, incl ... | 1997 | 9126699 |
| absence of burkholderia cepacia from the respiratory tract of non-cystic fibrosis patients. | | 1997 | 9131333 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| the effects of panresistant bacteria in cystic fibrosis patients on lung transplant outcome. | the number of cystic fibrosis (cf) patients undergoing lung transplant continues to rise as long term survival improves. one major contraindication to this potentially life-saving intervention is infection with multi-drug-resistant bacteria. we undertook this retrospective study in 66 transplanted patients over 6 yr to determine the influence of panresistant bacteria on transplant outcome. the in vitro antibiotic susceptibility pattern of respiratory tract bacteria obtained pre- and/or intraoper ... | 1997 | 9154879 |
| identification and characterization of a novel dna marker associated with epidemic burkholderia cepacia strains recovered from patients with cystic fibrosis. | burkholderia cepacia is a problematic pathogen that may spread among patients with cystic fibrosis (cf). one highly infectious cf strain that causes epidemics in both the united kingdom and eastern canada has been shown to possess both the cable pilin subunit gene (cbla) and a unique combination of insertion sequences. however, no genetic markers linking this strain type with other types epidemic at various centers have been identified. using a randomly amplified polymorphic dna (rapd) typing sc ... | 1997 | 9157133 |
| cloning, characterization, and sequence analysis of the clce gene encoding the maleylacetate reductase of pseudomonas sp. strain b13. | a 3,167-bp psti fragment of genomic dna from pseudomonas sp. strain b13 was cloned and sequenced. the gene clce consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 da which showed maleylacetate reductase activity. the protein had significant sequence similarities with the polypeptides encoded by tcbf of pp51 (59.4% identical positions), tfdf of pjp4 (55.1%), and tfte of burkholderia cepacia ac1100 (53.1%). the function of tcbf as maleylacetate red ... | 1997 | 9171435 |
| structure of catechol 2,3-dioxygenase gene encoded in tom plasmid of pseudomonas cepacia g4. | the catechol 2,3-dioxygenase is an extradiol-type dioxygenase which cleaves the c-c bond at the meta position of catechol to form 2-hydroxymuconic semialdehyde. a catechol 2,3-dioxygenase gene (tomb) in the tom plasmid of p. cepacia g4 has been cloned and its nucleotide sequence was analyzed. the enzyme gene consisted of 945 base pairs with an atg initiation codon and a tga termination codon which can encode a polypeptide of molecular weight 35 kda containing 314 amino acid residues, and a putat ... | 1997 | 9175755 |
| affinity and response of burkholderia pseudomallei and burkholderia cepacia to insulin. | the cells of burkholderia pseudomallei, b. cepacia and pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. the former two species were stained positively indicating insulin binding but p. aeruginosa was not. insulin exposure reduced phospholipase c and acid phosphatase activities of b. pseudomallei but did not affect those enzymatic activities of b. cepacia in the employed experimental conditions. it is suggested that b. pseudomallei have insulin receptor ... | 1996 | 9185275 |
| functional analyses of a variety of chimeric dioxygenases constructed from two biphenyl dioxygenases that are similar structurally but different functionally. | the biphenyl dioxygenases (bp dox) of strains pseudomonas pseudoalcaligenes kf707 and pseudomonas cepacia lb400 exhibit a distinct difference in substrate ranges of polychlorinated biphenyls (pcb) despite nearly identical amino acid sequences. the range of congeners oxidized by lb400 bp dox is much wider than that oxidized by kf707 bp dox. the pcb degradation abilities of these bp dox were highly dependent on the recognition of the chlorinated rings and the sites of oxygen activation. the kf707 ... | 1997 | 9190809 |
| phylogenetic and physiological comparisons of pah-degrading bacteria from geographically diverse soils. | the diversity of bacteria isolated from creosote- contaminated soils in the united states, norway, and germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (pahs), their phospholipid ester-linked fatty acid (gc-fame) profiles, sole carbon source utilization patterns (biolog assays), and 16s rrna sequences. bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. many were capable of degrading a broad range of the pahs found in cr ... | 1997 | 9195008 |
| caesarean section in a parturient with respiratory failure caused by cystic fibrosis. | we describe a 27-year-old primigravida suffering from cystic fibrosis. her chest was colonised with burkholderia cepacia and she was in respiratory failure for which she required constant nasal intermittent positive pressure ventilation. in view of her rapid deterioration, caesarean section was performed under epidural anaesthesia at 25 weeks gestation. a live 790-g boy was delivered. post-operatively she made steady progress for 5 days although still requiring nasal ventilatory support. thereaf ... | 1997 | 9203887 |
| subdural empyema due to burkholderia cepacia: an unusual complication after lung transplantation for cystic fibrosis. | | 1997 | 9204013 |
| structural elucidation of two polysaccharides present in the lipopolysaccharide of a clinical isolate of burkholderia cepacia. | based on the sugar composition, methylation analyses and smith degradation, supported by nmr spectroscopic analyses and fast-atom-bombardment ms experiments, the lipopolysaccharide produced by a clinical isolate of burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units; a major, containing d-rhamnose and d-galactose residues (2:1) with the structure -->3)-alpha-d-rhap(1-->3)-alpha-d-rhap(1-->4)-alpha-d-galp(1 --> (major), and a minor repea ... | 1997 | 9208925 |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| a novel means to develop strain-specific dna probes for detecting bacteria in the environment. | a simple means to develop strain-specific dna probes for use in monitoring the movement and survival of bacteria in natural and laboratory ecosystems was developed. the method employed amplification of genomic dna via repetitive sequence-based pcr (rep-pcr) using primers specific for repetitive extragenic palindromic (rep) elements, followed by cloning of the amplified fragments. the cloned fragments were screened to identify those which were strain specific, and these were used as probes for to ... | 1997 | 9212434 |
| porin from the halophilic species ectothiorhodospira vacuolata: cloning, structure of the gene and comparison with other porins. | the gene coding for the anion-specific porin of the halophilic eubacterium ectothiorhodospira (ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. it encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. comparison with aa sequences of porins from several other members of the proteobacteria revealed little homology. only two regions showed local homology with the previously sequen ... | 1997 | 9218724 |
| genotypic analysis of burkholderia cepacia isolates from 13 french cystic fibrosis centers. | burkholderia cepacia has been involved in outbreaks of pulmonary infection among patients with cystic fibrosis (cf), and the spread of a highly transmissible clone has been reported throughout the united kingdom and canada. these data prompted a dna-based typing study of the strains recovered in french cf centers. ninety-five isolates recovered from 71 patients attending 13 cf centers in 9 regions of france were characterized by randomly amplified polymorphic dna (rapd) analysis and pulsed-field ... | 1997 | 9230381 |
| use of bronchoalveolar lavage semiquantitative cultures in cystic fibrosis. | to assess bronchoalveolar lavage (bal) in adult cf patients with respiratory symptoms, we studied bal fluid (balf) culture results from 28 bronchoscopies in 11 patients. patients were asked to provide sputum for culture. all but two patients were receiving antibiotics at the time of bronchoscopy, with 13 bronchoscopies done on patients who had been receiving antibiotics for more than 10 d. gram stain of the balf was positive in 18 cases. in all but one balf, > 10,000 colony-forming units per mil ... | 1997 | 9230762 |
| contamination of diagnostic ophthalmic solutions in primary eye care settings. | pharmaceutical agents and irrigating solutions are widely used in both optometric and ophthalmologic practices. contamination of these containers or solutions could possibly pose some risk of infection to a patient. we set out to investigate the possible contamination of a representative sample of these containers in small office practices. representative bottles of two diagnostic pharmaceutical agents and an irrigating solution were obtained from primary care optometric and ophthalmologic pract ... | 1997 | 9232983 |
| the clonal antibody response to pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients. | the groel protein of pseudomonas aeruginosa belongs to the bacterial 60-65 kda heat shock protein family. a strong antibody response to groel has been found in cystic fibrosis (cf) patients with chronic pulmonary infection caused by p. aeruginosa. clonotypes of igg1 and igg2 antibodies against groel were studied in 60 consecutive sera from 18 cf patients with chronic p. aeruginosa infection using isoelectric focusing in combination with affinity immunoblotting. the persistent antigenic stimulati ... | 1997 | 9236862 |
| activity of pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support. | the purified lipase from pseudomonas cepacia was used as free and immobilized enzyme preparation for hydrolysis of p-nitrophenyl palmitate (pnpp) and p-nitrophenyl acetate (pnpa) in organic media. the free enzyme was mixed with bovine serum albumin and lyophilized. immobilization was on porous polypropylene. conditions where diffusional limitations of the substrate were not limiting the reaction rate were defined. the specific activity of the lipase was greatly enhanced upon immobilization: 16.5 ... | 1997 | 9237387 |
| low bacterial contamination of nebulizers in home treatment of cystic fibrosis patients. | many reports have shown bacterial contamination of nebulizers used by patients with cystic fibrosis (cf) at home. at the stockholm cf centre we recommend dismantling the equipment, washing, rinsing and drying it after each use, and disinfecting it once daily by boiling water or by 2% acetic acid followed by drying without rinsing. we studied whether patients comply with these recommendations and whether they are sufficient to prevent bacterial contamination. nebulizers from 49 cf patients were i ... | 1997 | 9253701 |
| microbiologic data overview of italian cystic fibrosis patients. | the aim of our study was to determine the frequency of microbiological culturing and prevalence of colonization by principal pathogens of the respiratory tract of italian cystic fibrosis patients. data on all italian cystic fibrosis patients were collected using a questionnaire sent to all italian cf centers. results were obtained of microbiological cultures from 2,521 patients. information was gained regarding the method of gathering biological samples, the percentage of patients undergoing mic ... | 1997 | 9258532 |
| chemoenzymatic synthesis of chiral biologically active heterocycles. | the chemoenzymatic approach to the preparation of some chiral biologically active heterocycles is discussed. synthetic strategies took advantage of enantioselective bioconversion processes carried out on suitable reaction intermediates. reductions of carbonyl compounds catalyzed by different alcohol dehydogenases (tbadh from thermoanaerobium brockii, 20 beta-hsdh from streptomyces hydrogenans, beta-hsdh from pseudomonas testosteroni) allowed the preparation with high enantiomeric purity of the e ... | 1997 | 9274001 |
| mobilization, cloning, and sequence determination of a plasmid-encoded polygalacturonase from a phytopathogenic burkholderia (pseudomonas) cepacia. | phytopathogenic strains of burkholderia cepacia (synonym pseudomonas cepacia) produce endopolygalacturonase, whereas strains of clinical and soil origin do not. growth of a phytopathogenic strain (atcc25416) at elevated temperatures resulted in nonpectolytic derivatives that were either cured of a resident plasmid or contained a plasmid of reduced mass. the resident 200-kb plasmid (ppec320) in strain atcc25416 was tagged with tn5-mob. the ppec320::tn5-mob (ppec321) plasmid was mobilized in b. ce ... | 1997 | 9304858 |
| priming of neutrophil respiratory burst activity by lipopolysaccharide from burkholderia cepacia. | neutrophil activation may play an important role in the pathogenesis of respiratory disease in burkholderia cepacia-colonized cystic fibrosis (cf) patients. as bacterial lipopolysaccharides (lps) are potent immunostimulatory molecules, we investigated the role of b. cepacia lps in neutrophil activation processes. lps extracted from a highly transmissible and virulent strain of b. cepacia (j2315) was found to increase neutrophil surface expression of the beta2 integrin, complement receptor 3, and ... | 1997 | 9317038 |
| a review of the in vitro activity of meropenem and comparative antimicrobial agents tested against 30,254 aerobic and anaerobic pathogens isolated world wide. | the in vitro activity of meropenem (formerly sm-7738), a new carbapenem, was compared with that of imipenem and five other broad-spectrum antimicrobials (ceftazidime, cefotaxime, piperacillin, piperacillin/tazobactam, and ciprofloxacin) against 30,254 clinically significant pathogens isolated in nine countries worldwide. overall, the carbapenems, meropenem and imipenem, were the most active drugs. meropenem was four- to 64-fold more active than imipenem against gram-negative bacteria, including ... | 1997 | 9327242 |
| in vitro activities of antimicrobial agents, alone and in combinations, against burkholderia cepacia isolated from blood. | burkholderia cepacia is a widespread, environmental gram-negative bacillus that is associated with nosocomial infections. this bacterium is considered to be an important pathogen in immunocompromised patients and is inherently resistant to multiple antimicrobial agents. to compare the activity of different antimicrobial agents and the potential of combinations against invasive strains of b. cepacia, we collected 36 isolates of b. cepacia from blood cultures and checked their susceptibilities to ... | 1997 | 9327247 |
| occurrence of multiple genomovars of burkholderia cepacia in cystic fibrosis patients and proposal of burkholderia multivorans sp. nov. | we performed an integrated genotypic and phenotypic analysis of 128 strains of the genera burkholderia, ralstonia, and pseudomonas in order to study the taxonomic structure of burkholderia cepacia and its relationships with other burkholderia species. our data show that presumed b. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as burkholderia vietnamiensis. this group of five phenotypically similar species is ... | 1997 | 9336927 |
| should patients with cystic fibrosis infected with burkholderia cepacia undergo lung transplantation? | | 1997 | 9337823 |
| genetic relatedness of burkholderia (pseudomonas) cepacia isolates from five cystic fibrosis centers in michigan. | burkholderia cepacia isolates from patients with cystic fibrosis (cf) attending five cf centers were studied for relatedness by cellular fatty acid methyl esters (fame) and by chromosomal dna restriction analysis. twenty-eight of 32 (87.5%) isolates tested were grouped in cluster group 1 based on their fame profiles. dna analysis revealed that 29 of 32 (90.6%) b. cepacia isolates from five cf centers had one closely related dna pattern. to examine strain variation over a time period, fame profil ... | 1997 | 9338052 |
| enhancement of burkholderia cepacia antimicrobial susceptibility by cationic compounds. | infections in cystic fibrosis (cf) due to burkholderia cepacia are challenging due to their resistance to antibiotics. we explored a new strategy for increasing the permeability of b. cepacia using cationic agents, including amino compounds, to reduce the mics of standard antibiotics. twenty-eight b. cepacia isolates from four cf centres in north america and four non-cf b. cepacia were examined by standard microtitre broth dilution methods for susceptibility to a variety of antibiotics in the pr ... | 1997 | 9338485 |
| influence of growth rate and nutrient limitation on susceptibility of burkholderia cepacia to ciprofloxacin and tobramycin. | the influence of growth rate and oxygen availability on ciprofloxacin and tobramycin sensitivity and cell surface hydrophobicity in burkholderia cepacia was assessed for cells grown in a chemostat under iron-limitation. whereas susceptibility to both antibiotics decreased with increasing growth rate and oxygen repletion yet increased under oxygen depletion, hydrophobicity decreased with increasing growth rate and oxygen repletion but was relatively unaffected under oxygen depletion. these result ... | 1997 | 9338495 |
| prolonged production of nadph oxidase-corrected granulocytes after gene therapy of chronic granulomatous disease. | little is known about the potential for engraftment of autologous hematopoietic stem cells in human adults not subjected to myeloablative conditioning regimens. five adult patients with the p47(phox) deficiency form of chronic granulomatous disease received intravenous infusions of autologous cd34(+) peripheral blood stem cells (pbscs) that had been transduced ex vivo with a recombinant retrovirus encoding normal p47(phox). although marrow conditioning was not given, functionally corrected granu ... | 1997 | 9342375 |
| the influence of moisture on microbial transport, survival and 2,4-d biodegradation with a genetically marked burkholderia cepacia in unsaturated soil columns. | the influence of moisture on the survival, movement and degradation activity of a 2,4-d degrading bacterium, burkholderia cepacia strain bri6001l, genetically engineered to contain bioluminescent and lactose utilization genes, was studied in unsaturated soil columns. the distance traveled by bri6001l was dependent on the clay content of the soil, higher clay contents being responsible for higher filtration coefficients. long term survival, in excess of one year, was attributed to strain bri6001l ... | 1997 | 9342881 |
| evaluation of preservative effectiveness in pharmaceutical products: the use of a wild strain of pseudomonas cepacia. | a sodium benzoate-sorbic acid preservative system of a pharmaceutical product was proved effective against a wild strain of pseudomonas cepacia, following the official method of the italian and british pharmacopoeias. however, this preservative system was ineffective against a challenge of ps. cepacia wild strain cells grown in the unpreserved pharmaceutical product and on culture media different from those described by the pharmacopoeias. the adaptive resistance of the wild strain of ps. cepaci ... | 1997 | 9351212 |
| biodiversity of a burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages. | a burkholderia cepacia population naturally occurring in the rhizosphere of zea mays was investigated in order to assess the degree of root association and microbial biodiversity at five stages of plant growth. the bacterial strains isolated on semiselective pcat medium were mostly assigned to the species b. cepacia by an analysis of the restriction patterns produced by amplified dna coding for 16s rrna (16s rdna) (ardra) with the enzyme alui. partial 16s rdna nucleotide sequences of some random ... | 1997 | 9361434 |
| green fluorescent protein as a marker for pseudomonas spp. | the development of sensitive methods for observing individual bacterial cells in a population in experimental models and natural environments, such as in biofilms or on plant roots, is of great importance for studying these systems. we report the construction of plasmids which constitutively express a bright mutant of the green fluorescent protein of the jellyfish aequorea victoria and are stably maintained in pseudomonas spp. we demonstrate the utility of these plasmids to detect individual cel ... | 1997 | 9361441 |
| characterization of the pena and penr genes of burkholderia cepacia 249 which encode the chromosomal class a penicillinase and its lysr-type transcriptional regulator. | burkholderia cepacia is recognized as an important pathogen in the lung infections of patients with cystic fibrosis. an inducible beta-lactamase activity has been associated with increased resistance to beta-lactam antibiotics in clinical isolates of b. cepacia. in this study, we report the revised sequence of the pena gene, which encodes the inducible penicillinase of b. cepacia, and show that it belongs to the molecular class a beta-lactamases and exhibits a high degree of similarity to the ch ... | 1997 | 9371340 |
| coob plays a chaperone-like role for the proteins involved in formation of cs1 pili of enterotoxigenic escherichia coli. | cs1 pili serve as the prototype of a class of filamentous appendages found on the surface of strains of enterotoxigenic escherichia coli. the four genes needed to synthesize functional cs1 pili in e. coli k12 are: cooa, which encodes the major pilin protein; cood, which encodes a minor pilin protein found at the tip of the structure; cooc, which encodes a protein found in the outer membrane of piliated bacteria; and coob. we show here that coob, which is required for pilus assembly but is not pa ... | 1997 | 9371833 |
| infections in patients with cystic fibrosis following lung transplantation. | there is controversy over whether colonization with drug-resistant organisms is a contraindication to lung transplantation. | 1997 | 9377954 |
| analysis of changes in congener selectivity during pcb degradation by burkholderia sp. strain tsn101 with increasing concentrations of pcb and characterization of the bphbcd genes and gene products. | we isolated and characterized a gram-negative bacterium, burkholderia sp. strain tsn101, that can degrade polychlorinated biphenyls (pcbs) at concentrations as high as 150 microg kaneclor 300/ml, a pcb mixture equivalent to aroclor 1242. growing cells of strain tsn101 degraded most of the tri- and tetrachlorobiphenyls in medium containing 25 microg kaneclor 300/ml. using pcb concentrations of 50-150 microg of kaneclor 300/ml, the congener selectivity pattern was different and the pattern of chlo ... | 1998 | 9396836 |
| lipopolysaccharide from burkholderia vietnamiensis strain lmg 6999 contains two polymers identical to those present in the reference strain for burkholderia cepacia serogroup o4. | lipopolysaccharide was isolated from strain lmg 6999 of burkholderia vietnamiensis. degradative and nmr spectroscopic studies established the presence of two polymeric fractions based on the following trisaccharide repeating units: i:-->3)-alpha-d-galp-(1-->3)-beta-d-galp-(1-->3)-beta-d-galpnac- (1-->; ii:-->3)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->4)- alpha-l-rhap-(1-->. the same polymers have previously been found together in lipopolysaccharide from the reference strain for burkholderia ... | 1997 | 9418254 |
| potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic groel homolog. | a groel homolog with a molecular mass of 60 kda, produced by the primary endosymbiotic bacterium (a buchnera sp.) of myzus persicae and released into the hemolymph, has previously been shown to be a key protein in the transmission of potato leafroll virus (plrv). like other luteoviruses and pea enation mosaic virus, plrv readily binds to extracellular buchnera groel, and in vivo interference in this interaction coincides with reduced capsid integrity and loss of infectivity. to gain more knowled ... | 1998 | 9420234 |
| microbial pathogenesis in cystic fibrosis: pulmonary clearance of mucoid pseudomonas aeruginosa and inflammation in a mouse model of repeated respiratory challenge. | chronic endobronchiolitis compounded by recurring pseudomonas aeruginosa infections is the major cause of morbidity and mortality in patients with cystic fibrosis (cf). in this study, a mouse model of repeated respiratory exposure to p. aeruginosa was established to facilitate investigations of factors contributing to p. aeruginosa persistence and associated inflammatory processes in the lung. while a single exposure to p. aeruginosa aerosols resulted in only mild histopathological changes, repe ... | 1998 | 9423869 |
| genetic characterization of insertion sequence isjp4 on plasmid pjp4 from ralstonia eutropha jmp134. | directly adjacent to the (tfdt-) tfdcdef gene cluster for chlorocatechol breakdown on plasmid pjp4 of ralstonia eutropha (formerly alcaligenes eutrophus) jmp134, we identified a 0.9-kb dna element, designated isjp4, with the typical features of a bacterial insertion sequence. isjp4 occurs as a single complete copy on plasmid pjp4. about 9 kb away from this copy, in the tfda-tfds intergenic region, we found a 71-bp duplication of the isjp4 right-hand extremity. in addition, we discovered a comple ... | 1997 | 9427552 |