| representative freshwater bacterioplankton isolated from crater lake, oregon. | high-throughput culturing (htc) methods that rely on dilution to extinction in very-low-nutrient media were used to obtain bacterial isolates from crater lake, oregon. 16s rrna sequence determination and phylogenetic reconstruction were used to determine the potential ecological significance of isolated bacteria, both in crater lake and globally. fifty-five crater lake isolates yielded 16 different 16s rrna gene sequences. thirty of 55 (55%) crater lake isolates had 16s rrna gene sequences with ... | 2004 | 15528517 |
| brassinosteroid deficiency due to truncated steroid 5alpha-reductase causes dwarfism in the lk mutant of pea. | the endogenous brassinosteroids in the dwarf mutant lk of pea (pisum sativum) were quantified by gas chromatography-selected ion monitoring. the levels of castasterone, 6-deoxocastasterone, and 6-deoxotyphasterol in lk shoots were reduced 4-, 70-, and 6-fold, respectively, compared with those of the wild type. the fact that the application of brassinolide restored the growth of the mutant indicated that the dwarf mutant lk is brassinosteroid deficient. gas chromatography-selected ion monitoring ... | 2004 | 15286289 |
| biphenyl dioxygenases: functional versatilities and directed evolution. | | 2004 | 15292119 |
| soil microbial community responses to additions of organic carbon substrates and heavy metals (pb and cr). | microcosm experiments were conducted with soils contaminated with heavy metals (pb and cr) and aromatic hydrocarbons to determine the effects of each upon microbial community structure and function. organic substrates were added as a driving force for change in the microbial community. glucose represented an energy source used by a broad variety of bacteria, whereas fewer soil species were expected to use xylene. the metal amendments were chosen to inhibit the acute rate of organic mineralizatio ... | 2005 | 16332740 |
| anesthetic interaction with ketosteroid isomerase: insights from molecular dynamics simulations. | the nature and the sites of interactions between anesthetic halothane and homodimeric delta5-3-ketosteroid isomerase (ksi) are characterized by flexible ligand docking and confirmed by 1h-15n nmr. the dynamics consequence of halothane interaction and the implication of the dynamic changes to ksi function are studied by multiple 5-ns molecular dynamics simulations in the presence and absence of halothane. both docking and md simulations show that halothane prefer the amphiphilic dimeric interface ... | 2005 | 16040747 |
| improved in situ hybridization efficiency with locked-nucleic-acid-incorporated dna probes. | low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rrna-targeted in situ hybridization. there are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rrna molecules. in this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (lna)-incorporated oligodeoxynucleotide probes (lna/dna probes) without compromising specificity. fluorescently ... | 2006 | 16885281 |
| microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. | the degradation of polycyclic aromatic hydrocarbons (pahs) by bacteria has been widely studied. while many pure cultures have been isolated and characterized for their ability to grow on pahs, limited information is available on the diversity of microbes involved in pah degradation in the environment. we have designed generic pcr primers targeting the gene fragment encoding the rieske iron sulfur center common to all pah dioxygenase enzymes. these rieske primers were employed to track dioxygenas ... | 2006 | 16751518 |
| medium- and short-chain dehydrogenase/reductase gene and protein families : the sdr superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes. | short-chain dehydrogenases/reductases (sdrs) constitute a large family of nad(p)(h)-dependent oxidoreductases, sharing sequence motifs and displaying similar mechanisms. sdr enzymes have critical roles in lipid, amino acid, carbohydrate, cofactor, hormone and xenobiotic metabolism as well as in redox sensor mechanisms. sequence identities are low, and the most conserved feature is an alpha/beta folding pattern with a central beta sheet flanked by 2 - 3 alpha-helices from each side, thus a classi ... | 2008 | 19011750 |
| mutational analysis of the stability of the h2a and h2b histone monomers. | the eukaryotic histone heterodimer h2a-h2b folds through an obligatory dimeric intermediate that forms in a nearly diffusion-limited association reaction in the stopped-flow dead time. it is unclear whether there is partial folding of the isolated monomers before association. to address the possible contributions of structure in the monomers to the rapid association, we characterized h2a and h2b monomers in the absence of their heterodimeric partner. by far-uv circular dichroism, the h2a and h2b ... | 2008 | 18976667 |
| protocol to determine accurate absorption coefficients for iron-containing transferrins. | an accurate protein concentration is an essential component of most biochemical experiments. the simplest method to determine a protein concentration is by measuring the a(280) using an absorption coefficient (epsilon) and applying the beer-lambert law. for some metalloproteins (including all transferrin family members), difficulties arise because metal binding contributes to the a(280) in a nonlinear manner. the edelhoch method is based on the assumption that the epsilon of a denatured protein ... | 2008 | 18471984 |
| a new classification system for bacterial rieske non-heme iron aromatic ring-hydroxylating oxygenases. | rieske non-heme iron aromatic ring-hydroxylating oxygenases (rhos) are multi-component enzyme systems that are remarkably diverse in bacteria isolated from diverse habitats. since the first classification in 1990, there has been a need to devise a new classification scheme for these enzymes because many rhos have been discovered, which do not belong to any group in the previous classification. here, we present a scheme for classification of rhos reflecting new sequence information and interactio ... | 2008 | 18387195 |
| hybrid quantum/classical molecular dynamics simulations of the proton transfer reactions catalyzed by ketosteroid isomerase: analysis of hydrogen bonding, conformational motions, and electrostatics. | hybrid quantum/classical molecular dynamics simulations of the two proton transfer reactions catalyzed by ketosteroid isomerase are presented. the potential energy surfaces for the proton transfer reactions are described with the empirical valence bond method. nuclear quantum effects of the transferring hydrogen increase the rates by a factor of approximately 8, and dynamical barrier recrossings decrease the rates by a factor of 3-4. for both proton transfer reactions, the donor-acceptor distanc ... | 2009 | 19799395 |
| cholesterol oxidase: physiological functions. | an important aspect of catalysis performed by cholesterol oxidase (3beta-hydroxysteroid oxidase) concerns the nature of its association with the lipid bilayer that contains the sterol substrate. efficient catalytic turnover is affected by the association of the protein with the membrane as well as the solubility of the substrate in the lipid bilayer. in this review, the binding of cholesterol oxidase to the lipid bilayer, its turnover of substrates presented in different physical environments, a ... | 2009 | 19843168 |
| high interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. | matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample) ... | 2009 | 19776231 |
| bioinformatic analyses of transmembrane transport: novel software for deducing protein phylogeny, topology, and evolution. | during the past decade, we have experienced a revolution in the biological sciences resulting from the flux of information generated by genome-sequencing efforts. our understanding of living organisms, the metabolic processes they catalyze, the genetic systems encoding cellular protein and stable rna constituents, and the pathological conditions caused by some of these organisms has greatly benefited from the availability of complete genomic sequences and the establishment of comprehensive datab ... | 2009 | 19776645 |
| the effects of individual pcb congeners on the soil bacterial community structure and the abundance of biphenyl dioxygenase genes. | polychlorinated biphenyls (pcbs) are toxic environmental contaminants that represent a class of 209 congeners characterized by different degrees of chlorination and substitution patterns. most of experimental studies about microbial degradation of pcbs have been conducted on pcb mixtures, even though evidence accumulated in bacteria and other organisms shows that exposure to different congeners may have different biological effects. microcosm experiments were conducted using aerobic agitated soi ... | 2009 | 19716603 |
| the effects of individual pcb congeners on the soil bacterial community structure and the abundance of biphenyl dioxygenase genes. | polychlorinated biphenyls (pcbs) are toxic environmental contaminants that represent a class of 209 congeners characterized by different degrees of chlorination and substitution patterns. most of experimental studies about microbial degradation of pcbs have been conducted on pcb mixtures, even though evidence accumulated in bacteria and other organisms shows that exposure to different congeners may have different biological effects. microcosm experiments were conducted using aerobic agitated soi ... | 2009 | 19716603 |
| bacterial degradation of aromatic compounds. | aromatic compounds are among the most prevalent and persistent pollutants in the environment. petroleum-contaminated soil and sediment commonly contain a mixture of polycyclic aromatic hydrocarbons (pahs) and heterocyclic aromatics. aromatics derived from industrial activities often have functional groups such as alkyls, halogens and nitro groups. biodegradation is a major mechanism of removal of organic pollutants from a contaminated site. this review focuses on bacterial degradation pathways o ... | 2009 | 19440284 |
| airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. | improvement in lung function after macrolide antibiotic therapy has been attributed to reduction in bronchial infection by specific bacteria. however, the airway might be populated by a more diverse microbiota, and clinical features of asthma might be associated with characteristics of the airway microbiota present. | 2010 | 21194740 |
| airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. | improvement in lung function after macrolide antibiotic therapy has been attributed to reduction in bronchial infection by specific bacteria. however, the airway might be populated by a more diverse microbiota, and clinical features of asthma might be associated with characteristics of the airway microbiota present. | 2010 | 21194740 |
| microbiological pattern of arterial catheters in the intensive care unit. | intravascular catheter related infection (cri) is one of the most serious nosocomial infections. diagnostic criteria include a positive culture from the catheter tip along with blood, yet in many patients with signs of infection, current culture techniques fail to identify pathogens on catheter segments. we hypothesised that a molecular examination of the bacterial community on short term arterial catheters (acs) would improve our understanding of the variety of organisms that are present in thi ... | 2010 | 20955628 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |
| antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river. | we characterized the bacterial populations in surface water receiving effluent from an oxytetracycline (otc) production plant. additional sampling sites included the receiving river water 5 km upstream and 20 km downstream from the discharge point. high levels of otc were found in the wastewater (ww), and the antibiotic was still detectable in river water downstream (rwd), with undetectable levels in river water upstream (rwu). a total of 341 bacterial strains were isolated using nonselective me ... | 2010 | 20400569 |
| phytoremediation of polychlorinated biphenyls: new trends and promises. | transgenic plants and associated bacteria constitute a new generation of genetically modified organisms for efficient and environment-friendly treatment of soil and water contaminated with polychlorinated biphenyls (pcbs). this review focuses on recent advances in phytoremediation for the treatment of pcbs, including the development of transgenic plants and associated bacteria. phytoremediation, or the use of higher plants for rehabilitation of soil and groundwater, is a promising strategy for c ... | 2010 | 20384372 |
| enatiomerically pure hydroxycarboxylic acids: current approaches and future perspectives. | the growing awareness of the importance of chirality in conjunction with biological activity has led to an increasing demand for efficient methods for the industrial synthesis of enantiomerically pure compounds. polyhydroxyalkanotes (phas) are a family of polyesters consisting of over 140 chiral r-hydroxycarboxylic acids (r-has), representing a promising source for obtaining chiral chemicals from renewable carbon sources. although some r-has have been produced for some time and certain knowledge ... | 2010 | 20393709 |
| the changing microbial epidemiology in cystic fibrosis. | infection of the airways remains the primary cause of morbidity and mortality in persons with cystic fibrosis (cf). this review describes salient features of the epidemiologies of microbial species that are involved in respiratory tract infection in cf. the apparently expanding spectrum of species causing infection in cf and recent changes in the incidences and prevalences of infection due to specific bacterial, fungal, and viral species are described. the challenges inherent in tracking and int ... | 2010 | 20375354 |
| evaluation of the energetics of the concerted acid-base mechanism in enzymatic catalysis: the case of ketosteroid isomerase. | structures of enzymes invariably reveal the proximity of acidic and basic residues to reactive sites on the substrate, so it is natural and common to suggest that enzymes employ concerted mechanisms to catalyze their difficult reactions. ketosteroid isomerase (ksi) has served as a paradigm of enzymatic proton transfer chemistry, and its catalytic effect has previously been attributed to concerted proton transfer. we employ a specific inhibitor that contains an ir probe that reports directly and ... | 2011 | 22148842 |
| evaluation of the energetics of the concerted acid-base mechanism in enzymatic catalysis: the case of ketosteroid isomerase. | structures of enzymes invariably reveal the proximity of acidic and basic residues to reactive sites on the substrate, so it is natural and common to suggest that enzymes employ concerted mechanisms to catalyze their difficult reactions. ketosteroid isomerase (ksi) has served as a paradigm of enzymatic proton transfer chemistry, and its catalytic effect has previously been attributed to concerted proton transfer. we employ a specific inhibitor that contains an ir probe that reports directly and ... | 2011 | 22148842 |
| evaluating the catalytic contribution from the oxyanion hole in ketosteroid isomerase. | prior site-directed mutagenesis studies in bacterial ketosteroid isomerase (ksi) reported that substitution of both oxyanion hole hydrogen bond donors gives a 10(5)- to 10(8)-fold rate reduction, suggesting that the oxyanion hole may provide the major contribution to ksi catalysis. but these seemingly conservative mutations replaced the oxyanion hole hydrogen bond donors with hydrophobic side chains that could lead to suboptimal solvation of the incipient oxyanion in the mutants, thereby potenti ... | 2011 | 22053826 |
| inducing mineral precipitation in groundwater by addition of phosphate. | induced precipitation of phosphate minerals to scavenge trace elements from groundwater is a potential remediation approach for contaminated aquifers. the success of engineered precipitation schemes depends on the particular phases generated, their rates of formation, and their long term stability. the purpose of this study was to examine the precipitation of calcium phosphate minerals under conditions representative of a natural groundwater. because microorganisms are present in groundwater, an ... | 2011 | 22029908 |
| clinical significance of microbial infection and adaptation in cystic fibrosis. | a select group of microorganisms inhabit the airways of individuals with cystic fibrosis. once established within the pulmonary environment in these patients, many of these microbes adapt by altering aspects of their structure and physiology. some of these microbes and adaptations are associated with more rapid deterioration in lung function and overall clinical status, whereas others appear to have little effect. here we review current evidence supporting or refuting a role for the different mi ... | 2011 | 21233507 |
| 16th carbonyl metabolism meeting: from enzymology to genomics. | the 16th international meeting on the enzymology and molecular biology of carbonyl metabolism, castle of ploen (schleswig-holstein, germany), july 10-15, 2012, covered all aspects of nad(p)-dependent oxido-reductases that are involved in the general metabolism of xenobiotic and physiological carbonyl compounds. starting 30 years ago with enzyme purification, structure elucidation and enzyme kinetics, the carbonyl society members have meanwhile established internationally recognized enzyme nomenc ... | 2012 | 23199258 |
| two transporters essential for reassimilation of novel cholate metabolites by rhodococcus jostii rha1. | the bacterial uptake of steroids and their metabolites remains poorly understood. we investigated two transporters associated with cholate catabolism in rhodococcus jostii rha1. reverse transcriptase quantitative-pcr indicated that an atp-binding cassette (abc) transporter and a major facilitator superfamily (mfs) transporter were upregulated 16.7- and 174-fold, respectively, during the exponential phase of growth on cholate compared to growth on pyruvate. gene knockout analysis established that ... | 2012 | 23024344 |
| self-subunit swapping occurs in another gene type of cobalt nitrile hydratase. | self-subunit swapping is one of the post-translational maturation of the cobalt-containing nitrile hydratase (co-nhase) family of enzymes. all of these nhases possess a gene organization of <β-subunit> <α-subunit> <activator protein>, which allows the activator protein to easily form a mediatory complex with the α-subunit of the nhase after translation. here, we discovered that the incorporation of cobalt into another type of co-nhase, with a gene organization of <α-subunit> <β-subunit> <activat ... | 2012 | 23226397 |
| denitrification performance and microbial community structure of a combined wla-obco system. | the contamination of surface water by nitrogen due to fertilizer application and discharge of wastewater is an increasingly serious problem. a multifunctional device, which combines water-lifting and aeration (wla) with oligotrophic biological contact oxidation (obco), was developed for pretreatment of raw water to reduce nitrogen. the performance of nitrogen removal and changes in microbial community structure were investigated. the results showed that the combined technique of wla-obco was fea ... | 2012 | 23152768 |
| application of a battery of biotests for the determination of leachate toxicity to bacteria and invertebrates from sewage sludge-amended soil. | the objective of the study was to determine the leachates toxicity from sewage sludge-amended soils (sandy and loamy). samples originated from a plot experiment realized over a period of 29 months. two types of soil were fertilized with sewage sludges at the dose of 3 % (90 t/ha). soil samples were taken after 0, 7, 17, and 29 months from the application of sewage sludges. leachates were obtained according to the en 12457-2 protocol. the following commercial tests were applied for the estimation ... | 2012 | 23132406 |
| application of a battery of biotests for the determination of leachate toxicity to bacteria and invertebrates from sewage sludge-amended soil. | the objective of the study was to determine the leachates toxicity from sewage sludge-amended soils (sandy and loamy). samples originated from a plot experiment realized over a period of 29 months. two types of soil were fertilized with sewage sludges at the dose of 3 % (90 t/ha). soil samples were taken after 0, 7, 17, and 29 months from the application of sewage sludges. leachates were obtained according to the en 12457-2 protocol. the following commercial tests were applied for the estimation ... | 2012 | 23132406 |
| acute infective endocarditis caused by delftia acidovorans, a rare pathogen complicating intravenous drug use. | gram-negative bacilli causing infective endocarditis (ie) is rare, even in intravenous drug users. this case report underscores several clinically important aspects of delftia acidovorans ie: the organism's ability to cause rapid destruction of normal native valves and to cause embolic occlusion of large arteries and its resistance to all aminoglycosides. | 2012 | 22933597 |
| nitrilases in nitrile biocatalysis: recent progress and forthcoming research. | over the past decades, nitrilases have drawn considerable attention because of their application in nitrile degradation as prominent biocatalysts. nitrilases are derived from bacteria, filamentous fungi, yeasts, and plants. in-depth investigations on their natural sources function mechanisms, enzyme structure, screening pathways, and biocatalytic properties have been conducted. moreover, the immobilization, purification, gene cloning and modifications of nitrilase have been dwelt upon. some nitr ... | 2012 | 23106943 |
| crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor. | dioxygen activation implemented by nonhaem fe(ii) enzymes containing the 2-his-1-carboxylate facial triad has been extensively studied in recent years. extradiol dioxygenase is the archetypal member of this superfamily and catalyzes the oxygenolytic ring opening of catechol analogues. here, the crystallization and preliminary x-ray analysis of 2-aminophenol 1,6-dioxygenase, an enzyme representing a minor subset of extradiol dioxygenases that catalyze the fission of 2-aminophenol rather than cate ... | 2012 | 23143244 |
| catabolism and biotechnological applications of cholesterol degrading bacteria. | cholesterol is a steroid commonly found in nature with a great relevance in biology, medicine and chemistry, playing an essential role as a structural component of animal cell membranes. the ubiquity of cholesterol in the environment has made it a reference biomarker for environmental pollution analysis and a common carbon source for different microorganisms, some of them being important pathogens such as mycobacterium tuberculosis. this work revises the accumulated biochemical and genetic knowl ... | 2012 | 22309478 |
| proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, arhodomonas sp. strain rozel, isolated from a hypersaline environment. | lately, there has been a special interest in understanding the role of halophilic and halotolerant organisms for their ability to degrade hydrocarbons. the focus of this study was to investigate the genes and enzymes involved in the initial steps of the benzene degradation pathway in halophiles. the extremely halophilic bacteria arhodomonas sp. strain seminole and arhodomonas sp. strain rozel, which degrade benzene and toluene as the sole carbon source at high salinity (0.5 to 4 m nacl), were is ... | 2012 | 22885747 |
| role of serine140 in the mode of action of mycobacterium tuberculosis β-ketoacyl-acp reductase (maba). | tuberculosis (tb) still remains one of the most deadly infectious diseases in the world. mycobacterium tuberculosis β-ketoacyl-acp reductase (maba) is a member of the fatty acid elongation system type ii, providing precursors of mycolic acids that are essential to the bacterial cell growth and survival. maba has been shown to be essential for m. tuberculosis survival and to play a role in intracellular signal transduction of bacilli. | 2012 | 23006410 |
| characterization of a novel phenol hydroxylase in indoles biotransformation from a strain arthrobacter sp. w1 [corrected]. | indigoids, as popular dyes, can be produced by microbial strains or enzymes catalysis. however, the new valuable products with their transformation mechanisms, especially inter-conversion among the intermediates and products have not been clearly identified yet. therefore, it is necessary to investigate novel microbial catalytic processes for indigoids production systematically. | 2012 | 23028517 |
| surviving chytridiomycosis: differential anti-batrachochytrium dendrobatidis activity in bacterial isolates from three lowland species of atelopus. | in the neotropics, almost every species of the stream-dwelling harlequin toads (genus atelopus) have experienced catastrophic declines. the persistence of lowland species of atelopus could be explained by the lower growth rate of batrachochytrium dendrobatidis (bd) at temperatures above 25 °c. we tested the complementary hypothesis that the toads' skin bacterial microbiota acts as a protective barrier against the pathogen, perhaps delaying or impeding the symptomatic phase of chytridiomycosis. w ... | 2012 | 22970314 |
| inactivation of chloramphenicol and florfenicol by a novel chloramphenicol hydrolase. | chloramphenicol and florfenicol are broad-spectrum antibiotics. although the bacterial resistance mechanisms to these antibiotics have been well documented, hydrolysis of these antibiotics has not been reported in detail. this study reports the hydrolysis of these two antibiotics by a specific hydrolase that is encoded by a gene identified from a soil metagenome. hydrolysis of chloramphenicol has been recognized in cell extracts of escherichia coli expressing a chloramphenicol acetate esterase g ... | 2012 | 22752166 |
| inferring the evolutionary history of incp-1 plasmids despite incongruence among backbone gene trees. | plasmids of the incompatibility group incp-1 can transfer and replicate in many genera of the proteobacteria. they are composed of backbone genes that encode a variety of essential functions and accessory genes that have implications for human health and environmental remediation. although it is well understood that the accessory genes are transferred horizontally between plasmids, recent studies have also provided examples of recombination in the backbone genes of incp-1 plasmids. as a conseque ... | 2012 | 22936717 |
| inferring the evolutionary history of incp-1 plasmids despite incongruence among backbone gene trees. | plasmids of the incompatibility group incp-1 can transfer and replicate in many genera of the proteobacteria. they are composed of backbone genes that encode a variety of essential functions and accessory genes that have implications for human health and environmental remediation. although it is well understood that the accessory genes are transferred horizontally between plasmids, recent studies have also provided examples of recombination in the backbone genes of incp-1 plasmids. as a conseque ... | 2012 | 22936717 |
| biodegradation of mono-hydroxylated pcbs by burkholderia xenovorans. | three hydroxylated derivatives of pcbs, 2'-hydroxy-4-chlorobiphenyl (2'-oh-4-cb), 3'-hydroxy-4-chlorobiphenyl (3'-oh-4-cb), and 4'-hydroxy-4-chlorobiphenyl (4'-oh-4-cb), were transformed by the pcb degrader, burkholderia xenovorans. when the bacterium was growing on biphenyl (biphenyl pathway-inducing conditions), all three hydroxylated isomers were transformed. however, only 2'-oh-4-cb was transformed by the bacterium growing on succinate (conditions non-inductive of the biphenyl pathway). gene ... | 2012 | 22918793 |
| recurrent microvirgula aerodenitrificans bacteremia. | microvirgula aerodenitrificans is a denitrifying gram-negative organism first described by patureau et al. in 1998 (d. patureau et al., int. j. syst. bacteriol. 48:775-782, 1998). the organism has been isolated globally but has never been described as causative of clinical infection. we describe the first human case of bacteremia attributed to m. aerodenitrificans in an infant with pompe's disease. | 2012 | 22692735 |
| isolation of a gene responsible for the oxidation of trans-anethole to para-anisaldehyde by pseudomonas putida jyr-1 and its expression in escherichia coli. | a plasmid, pta163, in escherichia coli contained an approximately 34-kb gene fragment from pseudomonas putida jyr-1 that included the genes responsible for the metabolism of trans-anethole to protocatechuic acid. three tn5-disrupted open reading frame 10 (orf 10) mutants of plasmid pta163 lost their abilities to catalyze trans-anethole. heterologously expressed orf 10 (1,047 nucleotides [nt]) under a t7 promoter in e. coli catalyzed oxidative cleavage of a propenyl group of trans-anethole to an ... | 2012 | 22610435 |
| identification of bacteria utilizing biphenyl, benzoate, and naphthalene in long-term contaminated soil. | bacteria were identified associated with biodegradation of aromatic pollutants biphenyl, benzoate, and naphthalene in a long-term polychlorinated biphenyl- and polyaromatic hydrocarbon-contaminated soil. in order to avoid biases of culture-based approaches, stable isotope probing was applied in combination with sequence analysis of 16 s rrna gene pyrotags amplified from (13)c-enriched dna fractions. special attention was paid to pyrosequencing data analysis in order to eliminate the errors cause ... | 2012 | 22808223 |
| marine crude-oil biodegradation: a central role for interspecies interactions. | the marine environment is highly susceptible to pollution by petroleum, and so it is important to understand how microorganisms degrade hydrocarbons, and thereby mitigate ecosystem damage. our understanding about the ecology, physiology, biochemistry and genetics of oil-degrading bacteria and fungi has increased greatly in recent decades; however, individual populations of microbes do not function alone in nature. the diverse array of hydrocarbons present in crude oil requires resource partition ... | 2012 | 22591596 |
| the phn island: a new genomic island encoding catabolism of polynuclear aromatic hydrocarbons. | bacteria are key in the biodegradation of polycyclic aromatic hydrocarbons (pah), which are widespread environmental pollutants. at least six genotypes of pah degraders are distinguishable via phylogenies of the ring-hydroxylating dioxygenase (rhd) that initiates bacterial pah metabolism. a given rhd genotype can be possessed by a variety of bacterial genera, suggesting horizontal gene transfer (hgt) is an important process for dissemination of pah-degrading genes. but, mechanisms of hgt for mos ... | 2012 | 22493593 |
| ring-cleaving dioxygenases with a cupin fold. | ring-cleaving dioxygenases catalyze key reactions in the aerobic microbial degradation of aromatic compounds. many pathways converge to catecholic intermediates, which are subject to ortho or meta cleavage by intradiol or extradiol dioxygenases, respectively. however, a number of degradation pathways proceed via noncatecholic hydroxy-substituted aromatic carboxylic acids like gentisate, salicylate, 1-hydroxy-2-naphthoate, or aminohydroxybenzoates. the ring-cleaving dioxygenases active toward the ... | 2012 | 22287012 |
| dynamics of the linuron hydrolase liba gene pool size in response to linuron application and environmental perturbations in agricultural soil and on-farm biopurification systems. | liba, a gene encoding a novel type of linuron hydrolase, was recently identified in the linuron-mineralizing variovorax sp. strain srs16. in order to assess the contribution of liba to linuron degradation in environmental settings, liba abundance was monitored in response to the application of linuron and to environmental perturbations in agricultural soil microcosms and microcosms simulating the matrix of on-farm biopurification systems. liba numbers were measured by real-time pcr and linked to ... | 2012 | 22307296 |
| biochemical characterization of the cdp-d-arabinitol biosynthetic pathway in streptococcus pneumoniae 17f. | streptococcus pneumoniae is a major human pathogen associated with many diseases worldwide. capsular polysaccharides (cpss) are the major virulence factor. the biosynthetic pathway of d-arabinitol, which is present in the cpss of several s. pneumoniae serotypes, has never been identified. in this study, the genes abpa (previously known as abp1) and abpb (previously known as abp2), which have previously been reported to be responsible for nucleoside diphosphate (ndp)-d-arabinitol (the nucleotide- ... | 2012 | 22328666 |
| molecular characterizations of cytolethal distending toxin produced by providencia alcalifaciens strains isolated from patients with diarrhea. | cytolethal distending toxins (cdts), which block eukaryotic cell proliferation by acting as inhibitory cyclomodulins, are produced by diverse groups of gram-negative bacteria. active cdt is composed of three polypeptides--cdta, cdtb, and cdtc--encoded by the genes cdta, cdtb, and cdtc, respectively. we developed a pcr-restriction fragment length polymorphism assay for the detection and differentiation of five alleles of cdtb (cdt-i through cdt-v) in escherichia coli and used the assay to investi ... | 2012 | 22252871 |
| phylogenetic and functional diversity of metagenomic libraries of phenol degrading sludge from petroleum refinery wastewater treatment system. | in petrochemical refinery wastewater treatment plants (wwtp), different concentrations of pollutant compounds are received daily in the influent stream, including significant amounts of phenolic compounds, creating propitious conditions for the development of particular microorganisms that can rapidly adapt to such environment. in the present work, the microbial sludge from a refinery wwtp was enriched for phenol, cloned into fosmid vectors and pyrosequenced. the fosmid libraries yielded 13,200 ... | 2012 | 22452812 |
| culturable gut microbiota diversity in zebrafish. | the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ... | 2012 | 22428747 |
| genome sequence of comamonas testosteroni atcc 11996, a representative strain involved in steroid degradation. | comamonas testosteroni strains belong to the family of comamonadaceae and are known for their ability to utilize steroid compounds as carbon source. here, we present the draft genome sequence of strain atcc 11996, with a g+c content of 61.48%. | 2012 | 22374961 |
| genes that influence swarming motility and biofilm formation in variovorax paradoxus eps. | variovorax paradoxus is an aerobic soil bacterium associated with important biodegradative processes in nature. we use v. paradoxus eps to study multicellular behaviors on surfaces. | 2012 | 22363744 |
| quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase. | understanding the electrostatic forces and features within highly heterogeneous, anisotropic, and chemically complex enzyme active sites and their connection to biological catalysis remains a longstanding challenge, in part due to the paucity of incisive experimental probes of electrostatic properties within proteins. to quantitatively assess the landscape of electrostatic fields at discrete locations and orientations within an enzyme active site, we have incorporated site-specific thiocyanate v ... | 2012 | 22308339 |
| microbes and asthma: the missing cellular and molecular links. | in this review, we describe the 'state-of-the-art' in our knowledge of asthma and what gaps exist, which can be exploited in the future for effective translation of our knowledge from the bench or population studies to diagnosis and therapy. | 2012 | 22113000 |
| metabolism of cholesterol and bile acids by the gut microbiota. | the human gastro-intestinal tract hosts a complex and diverse microbial community, whose collective genetic coding capacity vastly exceeds that of the human genome. as a consequence, the gut microbiota produces metabolites from a large range of molecules that host's enzymes are not able to convert. among these molecules, two main classes of steroids, cholesterol and bile acids, denote two different examples of bacterial metabolism in the gut. therefore, cholesterol is mainly converted into copro ... | 2013 | 25437605 |
| hexavalent chromium reduction under fermentative conditions with lactate stimulated native microbial communities. | microbial reduction of toxic hexavalent chromium (cr(vi)) in-situ is a plausible bioremediation strategy in electron-acceptor limited environments. however, higher [cr(vi)] may impose stress on syntrophic communities and impact community structure and function. the study objectives were to understand the impacts of cr(vi) concentrations on community structure and on the cr(vi)-reduction potential of groundwater communities at hanford, wa. steady state continuous flow bioreactors were used to gro ... | 2013 | 24376771 |
| poly(4-hydroxybutyrate) (p4hb) production in recombinant escherichia coli: p4hb synthesis is uncoupled with cell growth. | poly(4-hydroxybutyrate) (p4hb), belonging to the family of bacterial polyhydroxyalkanoates (phas), is a strong, flexible and absorbable material which has a large variety of medical applications like tissue engineering and drug delivery. for efficient production of p4hb recombinant escherichia coli has been employed. it was previously found that the p4hb synthesis is co-related with the cell growth. in this study, we aimed to investigate the physiology of p4hb synthesis, and to reduce the total ... | 2013 | 24325175 |
| comamonas testosteroni: an unusual bacteria associated with acute appendicitis. | | 2013 | 25207159 |
| the novel bacterial n-demethylase pdmab is responsible for the initial step of n,n-dimethyl-substituted phenylurea herbicide degradation. | the environmental fate of phenylurea herbicides has received considerable attention in recent decades. the microbial metabolism of n,n-dimethyl-substituted phenylurea herbicides can generally be initiated by mono-n-demethylation. in this study, the molecular basis for this process was revealed. the pdmab genes in sphingobium sp. strain ybl2 were shown to be responsible for the initial mono-n-demethylation of commonly used n,n-dimethyl-substituted phenylurea herbicides. pdmab is the oxygenase com ... | 2013 | 24123738 |
| biofilm development and enhanced stress resistance of a model, mixed-species community biofilm. | most studies of biofilm biology have taken a reductionist approach, where single-species biofilms have been extensively investigated. however, biofilms in nature mostly comprise multiple species, where interspecies interactions can shape the development, structure and function of these communities differently from biofilm populations. hence, a reproducible mixed-species biofilm comprising pseudomonas aeruginosa, pseudomonas protegens and klebsiella pneumoniae was adapted to study how interspecie ... | 2013 | 24152718 |
| uncovering the determinants of a highly perturbed tyrosine pka in the active site of ketosteroid isomerase. | within the idiosyncratic enzyme active-site environment, side chain and ligand pka values can be profoundly perturbed relative to their values in aqueous solution. whereas structural inspection of systems has often attributed perturbed pka values to dominant contributions from placement near charged groups or within hydrophobic pockets, tyr57 of a pseudomonas putida ketosteroid isomerase (ksi) mutant, suggested to have a pka perturbed by nearly 4 units to 6.3, is situated within a solvent-expose ... | 2013 | 24151972 |
| genomic and functional analyses of the 2-aminophenol catabolic pathway and partial conversion of its substrate into picolinic acid in burkholderia xenovorans lb400. | 2-aminophenol (2-ap) is a toxic nitrogen-containing aromatic pollutant. burkholderia xenovorans lb400 possess an amn gene cluster that encodes the 2-ap catabolic pathway. in this report, the functionality of the 2-aminophenol pathway of b. xenovorans strain lb400 was analyzed. the amnrjbacdfehg cluster located at chromosome 1 encodes the enzymes for the degradation of 2-aminophenol. the absence of haba and habb genes in lb400 genome correlates with its no growth on nitrobenzene. rt-pcr analyses ... | 2013 | 24124510 |
| n-sulfotestosteronan, a novel substrate for heparan sulfate 6-o-sulfotransferases and its analysis by oxidative degradation. | testosteronan, an unusual glycosaminoglycan (gag) first isolated from the microbe comamonas testosteroni, was enzymatically synthesized in vitro by transferring uridine diphosphate sugars on β-p-nitrophenyl glucuronide acceptor. after chemically converting testosteronan to n-sulfotestosteronan it was tested as a substrate for sulfotransferases involved in the biosynthesis of the gag, heparan sulfate. studies using (35) s-labeled 3'-phosphoadenosine-5'-phosphosulfate (paps) showed that only 6-o-s ... | 2013 | 23606289 |
| mhpt encodes an active transporter involved in 3-(3-hydroxyphenyl)propionate catabolism by escherichia coli k-12. | escherichia coli k-12 utilizes 3-(3-hydroxyphenyl)propionate (3hpp) as a sole carbon and energy source. among the genes in its catabolic cluster in the genome, mhpt was proposed to encode a hypothetical transporter. since no transporter for 3hpp uptake has been identified, we investigated whether mhpt is responsible for 3hpp uptake. mhpt fused with green fluorescent protein was found to be located at the periphery of cells by confocal microscopy, consistent with localization to the cytoplasmic m ... | 2013 | 23934492 |
| function of a glutamine synthetase-like protein in bacterial aniline oxidation via γ-glutamylanilide. | acinetobacter sp. strain yaa has five genes (atda1 to atda5) involved in aniline oxidation as a part of the aniline degradation gene cluster. from sequence analysis, the five genes were expected to encode a glutamine synthetase (gs)-like protein (atda1), a glutamine amidotransferase-like protein (atda2), and an aromatic compound dioxygenase (atda3, atda4, and atda5) (m. takeo, t. fujii, and y. maeda, j. ferment. bioeng. 85:17-24, 1998). a recombinant pseudomonas strain harboring these five genes ... | 2013 | 23893114 |
| characterizing the promiscuity of ligab, a lignin catabolite degrading extradiol dioxygenase from sphingomonas paucimobilis syk-6. | ligab from sphingomonas paucimobilis syk-6 is the only structurally characterized dioxygenase of the largely uncharacterized superfamily of type ii extradiol dioxygenases (edo). this enzyme catalyzes the oxidative ring-opening of protocatechuate (3,4-dihydroxybenzoic acid or pca) in a pathway allowing the degradation of lignin derived aromatic compounds (ldacs). ligab has also been shown to utilize two other ldacs from the same metabolic pathway as substrates, gallate, and 3-o-methyl gallate; ho ... | 2013 | 23977959 |
| improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by comamonas sp. eb172 utilizing volatile fatty acids by regulating the nitrogen source. | this study presents the effect of carbon to nitrogen ratio (c/n) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by comamonas sp. eb172 in 2 l fermenters using volatile fatty acids (vfa) as the carbon source. this vfa was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve c/n (mol/mol) 5, 15, 25, and 34.4, respectively. by extrapolating the c/n and the source of nitrogen, the properties of the polymers can be regula ... | 2013 | 24106698 |
| comamonas testosteroni peritonitis secondary to dislocated intrauterine device and laparoscopic intervention in a continuous ambulatory peritoneal dialysis patient. | | 2013 | 24133084 |
| environmental dissolved organic matter governs biofilm formation and subsequent linuron degradation activity of a linuron-degrading bacterial consortium. | it was examined whether biofilm growth on dissolved organic matter (dom) of a three-species consortium whose members synergistically degrade the phenylurea herbicide linuron affected the consortium's integrity and subsequent linuron-degrading functionality. citrate as a model dom and three environmental dom (edom) formulations of different quality were used. biofilms developed with all dom formulations, and the three species were retained in the biofilm. however, biofilm biomass, species composi ... | 2013 | 23666338 |
| evolution in action: dissemination of tet(x) into pathogenic microbiota. | | 2013 | 23847612 |
| identification of blaoxa-₅₁-like, blaoxa-₅₈, bladim-₁, and blavim carbapenemase genes in hospital enterobacteriaceae isolates from sierra leone. | we describe the results of a molecular epidemiological survey of 15 carbapenemase-encoding genes from a recent collection of clinical isolates from mercy hospital in bo, sierra leone. the most salient findings revealed that (i) 60% of the isolates harbored multiple carbapenemase genes; (ii) the bladim-1 gene, which has previously only been reported in the netherlands, is also circulating in this environment; and (iii) blaoxa-51-like and blaoxa-58 genes, which were thought to reside exclusively i ... | 2013 | 23658259 |
| use of anion-aromatic interactions to position the general base in the ketosteroid isomerase active site. | although the cation-pi pair, formed between a side chain or substrate cation and the negative electrostatic potential of a pi system on the face of an aromatic ring, has been widely discussed and has been shown to be important in protein structure and protein-ligand interactions, there has been little discussion of the potential structural and functional importance in proteins of the related anion-aromatic pair (i.e., interaction of a negatively charged group with the positive electrostatic pote ... | 2013 | 23798413 |
| quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site. | hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. we have carried out in-depth interrogations of the proton transfer equilibrium within a hydrogen bond network formed to bound phenols in the active site of ketosteroid isomerase. we systematically varied the proton affinity of the phenol using differing electron-withdrawing substituents and incorporated site-specific nmr and ir probes to quantitativ ... | 2013 | 23798390 |
| an oxygenase-independent cholesterol catabolic pathway operates under oxic conditions. | cholesterol is one of the most ubiquitous compounds in nature. the 9,10-seco-pathway for the aerobic degradation of cholesterol was established thirty years ago. this pathway is characterized by the extensive use of oxygen and oxygenases for substrate activation and ring fission. the classical pathway was the only catabolic pathway adopted by all studies on cholesterol-degrading bacteria. sterolibacterium denitrificans can degrade cholesterol regardless of the presence of oxygen. here, we aerobi ... | 2013 | 23826110 |
| the pathochip, a functional gene array for assessing pathogenic properties of diverse microbial communities. | pathogens present in the environment pose a serious threat to human, plant and animal health as evidenced by recent outbreaks. as many pathogens can survive and proliferate in the environment, it is important to understand their population dynamics and pathogenic potential in the environment. to assess pathogenic potential in diverse habitats, we developed a functional gene array, the pathochip, constructed with key virulence genes related to major virulence factors, such as adherence, colonizat ... | 2013 | 23765101 |
| rescue of deleterious mutations by the compensatory y30f mutation in ketosteroid isomerase. | proteins have evolved to compensate for detrimental mutations. however, compensatory mechanisms for protein defects are not well understood. using ketosteroid isomerase (ksi), we investigated how second-site mutations could recover defective mutant function and stability. previous results revealed that the y30f mutation rescued the y14f, y55f and y14f/y55f mutants by increasing the catalytic activity by 23-, 3- and 1.3-fold, respectively, and the y55f mutant by increasing the stability by 3.3 kc ... | 2013 | 23740430 |
| intra-abdominal infections due to comamonas kerstersii. | herein, we report four cases of comamonas kerstersii intra-abdominal infections representing the first report of human infections caused by this comamonas species. in addition, our work demonstrates the association of c. kerstersii with peritonitis secondary to appendix rupture. | 2013 | 23576541 |
| permanent draft genome sequence of comamonas testosteroni kf-1. | comamonas testosteroni kf-1 is a model organism for the elucidation of the novel biochemical degradation pathways for xenobiotic 4-sulfophenylcarboxylates (spc) formed during biodegradation of synthetic 4-sulfophenylalkane surfactants (linear alkylbenzenesulfonates, las) by bacterial communities. here we describe the features of this organism, together with the complete genome sequence and annotation. the 6,026,527 bp long chromosome (one sequencing gap) exhibits an average g+c content of 61.79% ... | 2013 | 23991256 |
| the catalytic roles of p185 and t188 and substrate-binding loop flexibility in 3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni. | 3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni reversibly catalyzes the oxidation of androsterone with nad(+) to form androstanedione and nadh. structurally the substrate-binding loop of the residues, t188-k208, is unresolved, while binding with nad(+) causes the appearance of t188-p191 in the binary complex. this study determines the functional roles of the flexible substrate-binding loop in conformational changes and enzyme catalysis. a stopped-flow study reveal ... | 2013 | 23717450 |
| bioavailability of heavy metals in soil: impact on microbial biodegradation of organic compounds and possible improvement strategies. | co-contamination of the environment with toxic chlorinated organic and heavy metal pollutants is one of the major problems facing industrialized nations today. heavy metals may inhibit biodegradation of chlorinated organics by interacting with enzymes directly involved in biodegradation or those involved in general metabolism. predictions of metal toxicity effects on organic pollutant biodegradation in co-contaminated soil and water environments is difficult since heavy metals may be present in ... | 2013 | 23676353 |
| hydroxylated polychlorinated biphenyls in the environment: sources, fate, and toxicities. | hydroxylated polychlorinated biphenyls (oh-pcbs) are produced in the environment by the oxidation of pcbs through a variety of mechanisms, including metabolic transformation in living organisms and abiotic reactions with hydroxyl radicals. as a consequence, oh-pcbs have been detected in a wide range of environmental samples, including animal tissues, water, and sediments. oh-pcbs have recently raised serious environmental concerns because they exert a variety of toxic effects at lower doses than ... | 2013 | 23636595 |
| anaerobic and aerobic cleavage of the steroid core ring structure by steroidobacter denitrificans. | the aerobic degradation of steroids by bacteria has been studied in some detail. in contrast, only little is known about the anaerobic steroid catabolism. steroidobacter denitrificans can utilize testosterone under both oxic and anoxic conditions. by conducting metabolomic investigations, we demonstrated that s. denitrificans adopts the 9,10-seco-pathway to degrade testosterone under oxic conditions. this pathway depends on the use of oxygenases for oxygenolytic ring fission. conversely, the det ... | 2013 | 23458847 |
| identification of genes and pathways related to phenol degradation in metagenomic libraries from petroleum refinery wastewater. | two fosmid libraries, totaling 13,200 clones, were obtained from bioreactor sludge of petroleum refinery wastewater treatment system. the library screening based on pcr and biological activity assays revealed more than 400 positive clones for phenol degradation. from these, 100 clones were randomly selected for pyrosequencing in order to evaluate the genetic potential of the microorganisms present in wastewater treatment plant for biodegradation, focusing mainly on novel genes and pathways of ph ... | 2013 | 23637911 |
| transformation of hydroxylated derivatives of 2,5-dichlorobiphenyl and 2,4,6-trichlorobiphenyl by burkholderia xenovorans lb400. | the polychlorinated biphenyl (pcb)-degrading bacterium, burkholderia xenovorans lb400, was capable of transforming three hydroxylated derivatives of 2,5-dichlorobiphenyl (2,5-dcb) (2'-hydroxy- (2'-oh-), 3'-oh-, and 4'-oh-2,5-dcb) when biphenyl was used as the carbon source (i.e., biphenyl pathway-inducing condition), although only 2'-oh-2,5-dcb was transformed when the bacterium was growing on succinate (i.e., condition non-inductive of the biphenyl pathway). on the contrary, hydroyxlated deriva ... | 2013 | 23589238 |
| two rnd proteins involved in heavy metal efflux in caulobacter crescentus belong to separate clusters within proteobacteria. | heavy metal resistance-nodulation-division (hme-rnd) efflux systems help gram-negative bacteria to keep the intracellular homeostasis under high metal concentrations. these proteins constitute the cytoplasmic membrane channel of the tripartite rnd transport systems. caulobacter crescentus na1000 possess two hme-rnd proteins, and the aim of this work was to determine their involvement in the response to cadmium, zinc, cobalt and nickel, and to analyze the phylogenetic distribution and characteris ... | 2013 | 23578014 |
| clinical and microbiological characteristics of perianal infections in adult patients with acute leukemia. | perianal infection is a common problem for patients with acute leukemia. however, neutropenia and bleeding tendency are relatively contraindicated to surgical intervention. the epidemiology, microbiology, clinical manifestations and outcomes of perianal infection in leukemic patients are also rarely discussed. | 2013 | 23577135 |
| five clinical cases of necropsobacter rosorum bacteremia. | five cases of bacteremia with necropsobacter rosorum are described, originating from intra-abdominal infections or localized soft tissue infections in the pelvic region. n. rosorum is consistently misidentified by commercial identification systems, which may delay recognition of this organism as a human pathogen. | 2013 | 23390273 |