| plant growth-promoting bacteria confer resistance in tomato plants to salt stress. | the object of the work is to evaluate whether rhizobacteria populating dry salty environments can increase resistance in tomato to salt stress. seven strains of plant growth-promoting bacteria that have 1-aminocyclopropane-1-carboxylate (acc) deaminase activity were isolated from soil samples taken from the arava region of southern israel. following growth of these seedlings in the presence of 43 mm nacl for 7 weeks, the bacterium that promoted growth to the greatest extent was selected for furt ... | 2004 | 15246071 |
| degradation of estradiol and ethinyl estradiol by activated sludge and by a defined mixed culture. | the aerobic degradation of the natural hormone 17-beta-estradiol (e2) and the synthetic hormone 17-alpha-ethinyl estradiol (ee2) was investigated in batch experiments with activated sludge from a conventional and a membrane sewage treatment plant. e2 was converted to estrone (e1), the well known metabolite, and further completely transformed within 3 days. the turnover rates of e2 did not differ greatly between conventional and membrane activated sludge. ee2 was persistent in both sludges. by se ... | 2005 | 15290133 |
| crystallization and preliminary x-ray diffraction analysis of two lysinal derivatives of achromobacter protease i. | two crystal forms of lysinal derivatives of achromobacter protease i have been obtained. the first, modified by benzyloxycarbonyl-val-lysinal crystallizes in the monoclinic space group p2(1) with unit-cell dimensions of a = 39.6, b = 71.2, c = 45.6 a and beta = 98.4 degrees. the second, modified by benzyloxycarbonyl-leu-leu-lysinal crystallizes in the orthorhombic space group i222 (or i2(1)2(1)2(1)) with unit-cell dimensions of a = 98.7, b = 102.2 and c = 55.8 a. the space groups and the unit-ce ... | 1996 | 15299616 |
| ial-chs (internal airlift loop--ceramic honeycomb supports) reactor used for biodegradation of 2,4-dichlorophenol and phenol. | the internal airlift loop reactor with ceramic honeycomb supports (ial-chs) was applied for biodegradation of 2,4-dichlorophenol (2,4-dcp) and phenol. a strain of dcp-degrading bacteria isolated from activated sludge, achromobacter sp., was rapidly immobilized onto the ceramic honeycomb supports. the immobilized cells effectively biodegraded 2,4-dcp alone and together with phenol in batch and continuous-flow experiments. for example, 2,4-dcp was biodegraded from an influent concentration of 50 m ... | 2004 | 15303748 |
| bacterial communities and enzyme activities of pahs polluted soils. | three soils (i.e. a belgian soil, b-bt, a german soil, g, and an italian agricultural soil, i-bt) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. a chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. the three soils showed different levels of polycyclic aromatic hydrocarbons (pahs), being their contamination ... | 2004 | 15331267 |
| mesophilic aerobic degradation of a metal lubricant by a biological consortium. | the metal-forming industries require the use of greases to lubricate metal surfaces during manufacturing operations, and the residues of these lubricants must be removed prior to finishing processes to protect and improve the appearance of the final product. an aqueous, biological metal-cleaning process operating under mild conditions (ph 9, 42 degrees c) eliminates the use of environmentally unfriendly cleaning materials such as chlorinated solvents by employing microorganisms to degrade grease ... | 2004 | 15378296 |
| bacteremia caused by achromobacter and alcaligenes species in 46 patients with cancer (1989-2003). | achromobacter and alcaligenes are emerging infectious gram-negative bacterial species that can affect immunosuppressed patients. the authors sought to determine the incidence and characteristics of bloodstream infections caused by these organisms in patients with underlying malignancies. | 2004 | 15389476 |
| structure-based engineering of alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin. | the intermolecular electron transfer from achromobacter cycloclastes pseudoazurin (acpaz) to wild-type and mutant alcaligenes xylosoxidans nitrite reductases (axnirs) was investigated using steady-state kinetics and electrochemical methods. the affinity and the electron transfer reaction constant (k(et)) are considerably lower between acpaz and axnir (k(m) = 1.34 mm and k(et) = 0.87 x 10(5) m(-1) s(-1)) than between acpaz and its cognate nitrite reductase (acnir) (k(m) = 20 microm and k(et) = 7. ... | 2004 | 15475344 |
| quantification of denitrifying bacteria in soils by nirk gene targeted real-time pcr. | denitrification, the reduction of nitrate to nitrous oxide or dinitrogen, is the major biological mechanism by which fixed nitrogen returns to the atmosphere from soil and water. microorganisms capable of denitrification are widely distributed in the environment but little is known about their abundance since quantification is performed using fastidious and time-consuming mpn-based approaches. we used real-time pcr to quantify the denitrifying nitrite reductase gene (nirk), a key enzyme of the d ... | 2004 | 15488276 |
| protein sequence analysis, cloning, and expression of flammutoxin, a pore-forming cytolysin from flammulina velutipes. maturation of dimeric precursor to monomeric active form by carboxyl-terminal truncation. | flammutoxin (ftx), a 31-kda pore-forming cytolysin from flammulina velutipes, is specifically expressed during the fruiting body formation. we cloned and expressed the cdna encoding a 272-residue protein with an identical n-terminal sequence with that of ftx but failed to obtain hemolytically active protein. this, together with the presence of multiple ftx family proteins in the mushroom, prompted us to determine the complete primary structure of ftx by protein sequence analysis. the n-terminal ... | 2004 | 15489231 |
| the completely sequenced plasmid pest4011 contains a novel incp1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation. | the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002 contains plasmid pest4011. this plasmid ensures its host a stable 2,4-d(+) phenotype. we determined the complete 76,958-bp nucleotide sequence of pest4011. this plasmid is a deletion and duplication derivative of pd2m4, the 95-kb highly unstable laboratory ancestor of pest4011, and was self-generated during different laboratory manipulations performed to increas ... | 2004 | 15489427 |
| amino acid sequence of a thrombin like enzyme, elegaxobin ii, from the venom of trimeresurus elegans (sakishima-habu). | the amino acid sequence of a thrombin like enzyme , named elegaxobin ii, isolated from the venom of trimeresurus elegans (sakishima-habu) was determined by edman sequencing of the peptides which was derived from digests with cyanogen bromide, achromobacter protease i, trypsin, endoproteinase asp-n, and chymotrypsin. elegaxobin ii consisted of 233 amino acids and showed conservation of the catalytic amino acid residues (his(57), asp(102), and ser(195)) of chymotrypsin family serine protease in it ... | 2004 | 15500847 |
| pitfalls of polymyxin antimicrobial susceptibility testing of pseudomonas aeruginosa isolated from cystic fibrosis patients. | with their potent activity against gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (cf) patients. a retrospective evaluation of polymyxin activity against 6001 pseudomonas aeruginosa, 150 achromobacter xylosoxidans and 506 stenotrophomonas maltophilia cf isolates was initiated. in addition, we looked at how polymyxin susceptibility testing was affected by the testing method (agar dilution versus microdilution), the agent (polymyxin e versus polymy ... | 2004 | 15509615 |
| characterization of two type 1 cu sites of hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases. | we report (1) the amino acid sequence of hyphomicrobium denitrificans nitrite reductase (hdnir), containing two type 1 cu sites and one type 2 cu site; (2) the expression and preparation of wild-type hdnir and two mutants replacing the cys ligand of each type 1 cu with ala; and (3) their spectroscopic and functional characterization. the open-reading frame of 50-kda hdnir is composed of the 15-kda n-terminal domain having a type 1 cu-binding motif like cupredoxins and the 35-kda c-terminal domai ... | 2004 | 15518568 |
| phosphonium ionic liquids for degradation of phenol in a two-phase partitioning bioreactor. | six ionic liquids (ils), which are organic salts that are liquid at room temperature, were tested for their biocompatibility with three xenobiotic-degrading bacteria, pseudomonas putida, achromobacter xylosoxidans, and sphingomonas aromaticivorans. of the 18 pairings, seven were found to demonstrate biocompatibility, with one il (trihexyl(tetradecyl)phosphonium bis(trifluoromethylsulfonyl) amide) being biocompatible with all three organisms. this il was then used in a two-phase partitioning bior ... | 2005 | 15549289 |
| cyclo(l-leucyl-l-prolyl) produced by achromobacter xylosoxidans inhibits aflatoxin production by aspergillus parasiticus. | aflatoxins are potent carcinogenic and toxic substances that are produced primarily by aspergillus flavus and aspergillus parasiticus. we found that a bacterium remarkably inhibited production of norsolorinic acid, a precursor of aflatoxin, by a. parasiticus. this bacterium was identified as achromobacter xylosoxidans based on its 16s ribosomal dna sequence and was designated a. xylosoxidans nfri-a1. a. xylosoxidans strains commonly showed similar inhibition. the inhibitory substance(s) was excr ... | 2004 | 15574949 |
| images in cardiovascular medicine. rare form of right ventricular outflow tract obstruction. | | 2004 | 15583085 |
| [biotransformation of organic substances by an immobilized associative bacterial culture]. | bacterial strains were screened to transform to end-products (carbon monoxide and water) elevated concentrations of acetone, acidic acid, and ethanol in a biocatalyst with an immobilized bacterial association cultivated on solid foam polyvinyl acetate (fpva). the innocuous association amalgamated paracoccus denitrificans vkm v-1324, pseudomonas esterophilus vkm v-1736d and achromobacter parvulus vkm v-1541d. the biocatalyst was tested with the help of classic methods and equipment for microbes c ... | 2004 | 15605736 |
| clonal relatedness and conserved integron structures in epidemiologically unrelated pseudomonas aeruginosa strains producing the vim-1 metallo-{beta}-lactamase from different italian hospitals. | three epidemiologically independent pseudomonas aeruginosa isolates, representative of the first vim-1 metallo-beta-lactamase producers detected at three different hospitals in northern italy, were investigated to determine their genomic relatedness and to compare the structures of the genetic supports for the vim-1 determinants. the three isolates, all of serotype o11, appeared to be clonally related according to the results of genotyping by macrorestriction analysis of genomic dna by pulsed-fi ... | 2005 | 15616282 |
| advenella incenata gen. nov., sp. nov., a novel member of the alcaligenaceae, isolated from various clinical samples. | a polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. phenotypically these isolates shared several characteristics with members of the alcaligenaceae and related genera. random amplified polymorphic dna fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by dna-dna hybridization experiments. 16s rrna gene sequence analysis indicated that these isolates were related ... | 2005 | 15653883 |
| quantifying maintenance requirements from the steady-state operation of a two-phase partitioning bioscrubber. | an innovative method for directly and explicitly quantifying the maintenance energy requirements of pure cultures growing on volatile organic compound (voc) substrates in a two-phase partitioning bioscrubber is described. direct evidence of maintenance energy requirements of achromobacter xylosoxidans y234 is provided both through observed reductions in the macroscopic biomass-to-substrate yield with decreasing specific growth rates, but more remarkably through achievement of steady-state operat ... | 2005 | 15726578 |
| technical aspects of gel-based proteomics designed for elucidating an aryl hydrocarbon receptor complex. | the identification of proteins by mass spectrometry has revolutionalized the basic method of identifying proteins constituting an intracellular unit or network for certain biological functions. the gel-based strategy following immunoprecipitation was applied to elucidating proteins associated with the aryl hydrocarbon receptor (ahr). two hundred femtomoles of ahr was recovered from approximately 2 x 10(7) hepg2 cells by immunoprecipitation and was sufficient for identification by peptide mass fi ... | 2004 | 15746886 |
| biodegradation of 2,4,6-tribromophenol during transport in fractured chalk. | the effect of physicochemical conditions (residence time, oxygen concentrations, and chalk characteristics) on the biodegradation of 2,4,6-tribromophenol (tbp) during transport was investigated in low-permeability fractured-chalk cores. long-term (approximately 600 d) biodegradation experiments were conducted in two cores (approximately 21 cm diameter, 31 and 44 cm long, respectively), intersected by a natural fracture. tbp was used as a model contaminant and as the sole carbon source for aerobi ... | 2005 | 15757335 |
| plant-microbe interactions: identification of epiphytic bacteria and their ability to alter leaf surface permeability. | bacteria were either isolated from leaf surfaces of hedera helix or obtained from a culture collection in order to analyse their effect on barrier properties of isolated hedera and prunus laurocerasus cuticles. on the basis of the 16s rdna sequences the genera of the six bacterial isolates from hedera were identified as pseudomonas sp., stenotrophomonas sp. and achromobacter. water permeability of cuticles isolated from h. helix was measured before and after inoculation with the six bacterial st ... | 2005 | 15819920 |
| [application of molecular systematics to study of bacterial cultures consuming volatile organic compounds]. | a range of species of four mixed bacterial cultures was studied by molecular systematics methods with the use of 16s rrna genes. the cultures had been developed for application in minireactors, to degrade volatile organic compounds (vocs): ethyl benzene, m-xylene, styrene, and o-xylene. a sample of 30 plasmid rdna clones was obtained for each of the mixed cultures. the clones were analyzed by rflp according to two restriction sites. major variants of the 16s-rdna sequences, corresponding to the ... | 2005 | 15859460 |
| pusillimonas noertemannii gen. nov., sp. nov., a new member of the family alcaligenaceae that degrades substituted salicylates. | the taxonomic position of a pseudomonas-like strain, designated bn9(t), was investigated. this strain had previously been isolated as a 5-aminosalicylate-degrading organism from a 6-aminonaphthalene-2-sulphonate-degrading mixed bacterial culture. previously, detection of ubiquinone q-8, a polyamine pattern with putrescine, spermidine and 2-hydroxyputrescine as the major polyamines, and partial 16s rrna gene sequencing had suggested that strain bn9(t) belongs to the 'betaproteobacteria'. this was ... | 2005 | 15879236 |
| dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase. | amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ... | 2005 | 15913357 |
| hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil. | we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ... | 2005 | 15920621 |
| high resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase. | we present high-resolution crystal structures and functional analysis of t1cu centre mutants of nitrite reductase that perturb the redox potential and the cys130-his129 "hard-wired" bridge through which electron transfer to the catalytic t2cu centre occurs. these data provide insight into how activity can be altered through mutational manipulation of the electron delivery centre (t1cu). the alteration of cys to ala results in loss of t1cu and enzyme inactivation with azurin as electron donor des ... | 2005 | 15927201 |
| shared genotypes of achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation center. | during a study examining transmission of pseudomonas aeruginosa among 76 cystic fibrosis patients in a rehabilitation center, where patients stay in close contact during prolonged periods, several clusters of patients carrying genotypically identical p. aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical achromobacter xylosoxidans strains, were discovered. | 2005 | 15956444 |
| a novel bacterium achromobacter xylosoxidans as a cause of liver abscess: three case reports. | achromobacter xylosoxidans is a microorganism that may cause opportunistic infections. we detected a. xylosoxidans in three of 46 patients with liver abscess. the clinicopathologic findings of the three patients were uniform with respect to a prior history of cholecystectomy, multi-lobulated 'coral-like' abscess under ct and epithelioid granulomas in the periphery of the abscess. achromobacter xylosoxidans is an unrecognized cause of liver abscess in humans. | 2005 | 15978693 |
| antimicrobial peptide therapeutics for cystic fibrosis. | greater than 90% of lung infections in cystic fibrosis (cf) patients are caused by pseudomonas aeruginosa, and the majority of these patients subsequently die from lung damage. current therapies are either targeted at reducing obstruction, reducing inflammation, or reducing infection. to identify potential therapeutic agents for the cf lung, 150 antimicrobial peptides consisting of three distinct structural classes were screened against mucoid and multidrug-resistant clinical isolates of p. aeru ... | 2005 | 15980369 |
| diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes. | seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera pseudaminobacter, achromobacter, brucella, and ochrobactrum. southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from plesiomonas sp. strain m6 and were all located on the chromosome. gene libraries were constructed from genomic dna of each of the 7 organophosphorus pesticide-degrading bacter ... | 2005 | 15980896 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |
| antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. | antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. of the 120 isolates pseudomonas spp. (55.8%) was the predominant group followed by acinetobacter spp. (14.17%), flavobacterium spp. (10.83%), achromobacter spp. (10%), burkholderia cepacia (3.3%), agrobacterium/radiobacter (2.5%), moraxella spp. (1.7%), aeromonas hydrophila (1.7%). over 80% of the isolates were resistant to one or more antibiotics and the highest resistance wa ... | 2005 | 15993695 |
| cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans. | amidases are very important enzymes for industrial biocatalysis. we scored a novel amidase by screening the achromobacter xylosoxidans gene library with cephalosporin analogous amides. the gene coding for the enzyme, designated ana, was cloned, sequenced and overexpressed in escherichia coli. sequence analysis of ana showed it to be an amidase signature family member. interestingly, we noted that almost all ana homologous amidases are from human pathogens responsible for chronic lung infections. ... | 2005 | 16002239 |
| taking the fungal highway: mobilization of pollutant-degrading bacteria by fungi. | the capacity of fungi to serve as vectors for the dispersion of pollutant-degrading bacteria was analyzed in laboratory model systems mimicking water-saturated (agar surfaces) and unsaturated soil environments (glass-bead-filled columns). two common soil fungi (fusarium oxysporum and rhexocercosporidium sp.) forming hydrophilic and hydrophobic mycelia, respectively, and three polycyclic aromatic hydrocarbon degrading bacteria (achromobacter sp. sk1, mycobacterium frederiksbergense lb501tg, and s ... | 2005 | 16047804 |
| [use of 16s rrna gene sequencing for identification of "pseudomonas-like" isolates from sputum of patients with cystic fibrosis]. | since nonfermenting, gram negative bacilli recovered from patients with cystic fibrosis could be misidentified with phenotypic procedures, we used partial 16s ribosomal rna gene (16s gene) sequencing to identify these "pseudomonas-like" isolates. 473 isolates were recovered from 66 patients in 2003. sequencing was used to identify 29 (from 24 patients) of the 473 isolates, showing unclear results with routine tests. pcr with specific primers was carried out to amplify a 995 bp fragment, which wa ... | 2005 | 16081224 |
| burkholderia and emerging pathogens in cystic fibrosis. | persons with cystic fibrosis (cf) are susceptible to respiratory tract infection with a variety of bacterial species. among these are several species in the genus burkholderia (including members of the b. cepacia complex, b. gladioli, and b. pseudomallei), stenotrophomonas maltophilia, achromobacter ( alcaligenes) xylosoxidans, certain ralstonia species, and species within the new genus pandoraea. enterobacteriaceae and several other rather unusual or novel species also can be found. many of the ... | 2003 | 16088584 |
| patient cohorting and infection control. | patients with cystic fibrosis (cf) have an abnormal propensity for recurrent and chronic infections of the lower respiratory tract (lrt), and the most common cause of a shortened lifespan is chronic infection with pseudomonas aeruginosa. a few other gram-negative organisms, primarily burkholderia cepacia complex have, however, emerged as serious pathogens capable of establishing chronic lrt infection. details of these and other cf pathogens can be found in the article by dr. john lipuma, burkhol ... | 2003 | 16088586 |
| atomic resolution structures of resting-state, substrate- and product-complexed cu-nitrite reductase provide insight into catalytic mechanism. | copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (no), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere. they are found in a wide variety of denitrifying bacteria and fungi of different physiology from a range of soil and aquatic ecosystems. structural analysis of potential intermediates in the catalytic cycle is an important goal in understanding enzyme mechanism. using "crystal harvesting" and substrate-soaki ... | 2005 | 16093314 |
| [alcaligenes xylosoxidans--a pathogen of chronic ear infection]. | we discuss a case of a 48-year old man admitted to our department with symptoms of chronic ear infection, that didn't answer to typical conservative treatment. the culture of the discharge was positive for alcaligenes xylosoxidans. | 2005 | 16095102 |
| characteristics of achromobacter xylosoxidans bacteremia in northern taiwan. | there were 40 cases of achromobacter xylosoxidans bacteremia during a 6-year period in a medical center in northern taiwan. males outnumbered females (72.5% vs 27.5%). common underlying diseases and conditions in these 40 bacteremic patients were malignancies (57.5%), central venous catheter implants (55%), surgery (35%), neutropenia (30%) and use of steroids (25%). recurrent bacteremia occurred in 4 patients (10%), all of whom had a central venous catheter implant which was considered the most ... | 2005 | 16118676 |
| roles of trp144 and tyr203 in copper-containing nitrite reductase from achromobacter cycloclastes iam1013. | the roles of the trp144 and tyr203 residues near the type 1 cu site of achromobacter cycloclastes nitrite reductase (acnir) have been examined with mutants of acnir. tyr203 is located on the protein surface near the type 1 cu site of acnir, and trp144 is between the tyr203 and the type 1 cu center in acnir. single mutation of trp144 or tyr203 in acnir to leu resulted in decreased rate constants of intermolecular electron transfer from its cognate pseudoazurin (acpaz) (k(et)=1.9x10(5), 2.2x10(5), ... | 2005 | 16125674 |
| tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in jammu and kashmir, india. | twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources and characterized on the basis of their physiological properties and ability to oxidize various reduced sulfur compounds. the new isolates could oxidize tetrathionate as well as thiosulfate, and oxidation of the latter involved conversion of thiosulfate to tetrathionate followed by its accumulation and eventual oxidation to sulfate, manifested in the production of ... | 2005 | 16166666 |
| degradation of natural rubber by achromobacter sp. nrb and evaluation of culture conditions. | a natural rubber degrading candidate was isolated from a soil sample from aswan, egypt. the strain was able to grow on natural rubber as a sole source for carbon and energy. according to its degradation behavior, it grew adhesively and in direct contact with the rubber substrate and led to disintegration of the material during cultivation. furthermore, this strain was not able to form a clear zone (translucent halos) around bacterial colonies after cultivation on nr latex plates. taxonomic analy ... | 2005 | 16209096 |
| a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans infection. | to report a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans. | 2005 | 16209287 |
| histidine 210 mutant of a trypsin-type achromobacter protease i shows broad optimum ph range. | achromobacter protease i (api), a lysine-specific serine protease, shows one order of magnitude higher activity than bovine trypsin, while its optimum ph is in the alkaline region at about ph 9. to improve the optimum ph range, mutant enzyme his 210 replaced by ser(h210s), ala (h210a), and lys (h210k) were constructed. the optimum ph of h210s shifted from about ph 9 (wild-type enzyme) to about ph 7, retaining its high activity. the putative electrostatic interaction between his 210 and catalytic ... | 2002 | 16233210 |
| purification, bacteriolytic activity, and specificity of beta-lytic protease from lysobacter sp. ib-9374. | lysobacter sp. ib-9374, which was isolated from soil as a high lysyl endopeptidase-producing strain (chohnanet al., fems microbiol. lett., 213, 13-20, 2002), was found to produce a beta-lytic protease capable of lysing gram-positive bacteria such as staphylococcus aureus, microccocuseus, and bacillus subtilis. the lysobacter strain secreted the beta-lytic protease into the culture medium at a 2.4-fold higher level than achromobacter lyticus. the enzyme was highly purified through a series of six ... | 2003 | 16233362 |
| imipenem-resistant achromobacter xylosoxidans carrying blavim-2-containing class 1 integron. | we characterized seven isolates of imipenem-resistant achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in korea during 2001 to 2003. from the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce vim-2, oxa-30, and chromosomal ampc beta-lactamase with a pi of 8.4. they showed a similar antibiogram, which were resistant to all tested aminoglycosides as w ... | 2005 | 16260111 |
| bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. | bacterial community dynamics and biodegradation processes were examined in a highly creosote-contaminated soil undergoing a range of laboratory-based bioremediation treatments. the dynamics of the eubacterial community, the number of heterotrophs and polycyclic aromatic hydrocarbon (pah) degraders, and the total petroleum hydrocarbon (tph) and pah concentrations were monitored during the bioremediation process. tph and pahs were significantly degraded in all treatments (72 to 79% and 83 to 87%, ... | 2005 | 16269736 |
| chronic granulomatous disease presenting with eosinophilic inflammation. | chronic granulomatous disease (cgd), a rare immunodeficiency, typically presents with recurrent infections caused by catalase-positive organisms. we report 2 patients with cgd who presented with eosinophilic inflammatory conditions recognized before the diagnosis of cgd. both patients had significant urologic disease. physicians should be aware of the association of cgd with eosinophilic inflammatory conditions. | 2005 | 16282946 |
| outbreak of long-term intravascular catheter-related bacteremia due to achromobacter xylosoxidans subspecies xylosoxidans in a hemodialysis unit. | achromobacter xylosoxidans is a rare cause of bacteremia. over a 2-week period, a. xylosoxidans subsp. xylosoxidans was isolated from blood cultures of four hemodialysis patients with long-term intravascular catheters. a culture from one atomizer that contained diluted 2.5% chlorhexidine, which had been used to disinfect the skin, yielded a. xylosoxidans subsp. xylosoxidans. no further cases were diagnosed once the use of this atomizer was discontinued. five outbreak-related strains from the fou ... | 2005 | 16283217 |
| investigation of hospital-acquired infections due to achromobacter xylosoxidans in a tertiary care hospital in india. | | 2006 | 16289456 |
| crystal structure of c-terminal desundecapeptide nitrite reductase from achromobacter cycloclastes. | monoclinic crystal structure of c-terminal desundecapeptide nitrite reductase (nirc-11) from achromobacter cycloclastes was determined at 2.6a. nirc-11 exists as a loose trimer in the crystal. deletion of 11 residues eliminates all intersubunit hydrogen bonds mediated by the c-terminal tail. the rigid irregular coil 105-112, which constitutes part of the sidewall of the active site pocket, undergoes conformational changes and becomes highly flexible in nirc-11. correspondingly, the linker segmen ... | 2005 | 16293231 |
| antimicrobial susceptibility patterns of unusual nonfermentative gram-negative bacilli isolated from latin america: report from the sentry antimicrobial surveillance program (1997-2002). | the antimicrobial susceptibility of 176 unusual non-fermentative gram-negative bacilli (nf-gnb) collected from latin america region through the sentry program between 1997 and 2002 was evaluated by broth microdilution according to the national committee for clinical laboratory standards (nccls) recommendations. nearly 74% of the nf-bgn belonged to the following genera/species: burkholderia spp. (83), achromobacter spp. (25), ralstonia pickettii (16), alcaligenes spp. (12), and cryseobacterium sp ... | 2005 | 16302068 |
| achromobacter xylosoxidans endophthalmitis diagnosed by polymerase chain reaction and gene sequencing. | to report a patient with achromobacter xylosoxidansendophthalmitis that was diagnosed using polymerase chain reaction and gene sequencing. | 2005 | 16321892 |
| transient performance of a two-phase partitioning bioscrubber treating a benzene-contaminated gas stream. | the dynamic performance of a prototype, two-phase partitioning bioscrubber in response to fluctuating benzene waste gas feeds has been characterized through a series of spike, step, and shutdown-restart experiments. from stable operation at a nominal loading capacity of 62 +/- 6 g/(m3 h) and removal efficiencies of over 99%, the bioscrubber was subjected to influent benzene concentration step changes and spikes as high as 10- and 20-fold, respectively. the bioscrubber responded rapidly and effec ... | 2005 | 16323802 |
| vim-2 metallo-beta-lactamase in achromobacter xylosoxidans in europe. | | 2005 | 16333608 |
| species composition and barotolerance of gut microflora of deep-sea benthic macrofauna collected at various depths in the atlantic ocean. | the bacterial flora of marine animals collected at depths of 570 to 2,446 m was examined for population size and generic composition, and the barotolerant characteristics of selected bacterial isolates were determined. total numbers of culturable, aerobic, heterotrophic bacteria were found to be low in animals collected at the greatest ocean depths sampled in this study. vibrio spp. were predominant in 10 of 15 samples examined, and photobacterium spp. and yeasts were the major components of the ... | 1980 | 16345648 |
| l-tryptophan production by achromobacter liquidum. | conditions for the production of tryptophanase from achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. the enzyme could be produced in amounts as great as 0.750 u/ml (degradation) and 0.294 u/ml (synthesis) by shaking cultures at 30 degrees c in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-tryptophan was produced most efficiently by shaking the cells at 37 degrees c in a reaction mixture containing 60 mg of l-ser ... | 1983 | 16346331 |
| relationship between cell surface properties and transport of bacteria through soil. | a study was conducted to relate the properties of enterobacter, pseudomonas, bacillus, achromobacter, flavobacterium, and arthrobacter strains to their transport with water moving through soil. the bacteria differed markedly in their extent of transport; their hydrophobicity, as measured by adherence to n-octane and by hydrophobic-interaction chromatography; and their net surface electrostatic charge, as determined by electrostatic interaction chromatography and by measurements of the zeta poten ... | 1991 | 16348394 |
| changes in the microflora of vacuum-packaged, irradiated petrale sole (eopsetta jordani) fillets stored at 0.5 c. | the microfloral changes of irradiated petrale sole fillets during anaerobic (vacuum-packed in cans) refrigerated storage was determined by the identification of 1,260 microbial isolates to the generic level. the samples were irradiated at 0.0, 0.1, 0.2, 0.3, and 0.4 mrad from a cobalt-60 gamma source, stored at 0.5 c, and examined periodically for spoilage and total microbial population and composition. the preirradiation flora consisted of achromobacter, micrococcus, pseudomonas, coryneforms, l ... | 1966 | 16349697 |
| effect of gamma irradiation on the microflora of freshwater fish: ii. generic identification of aerobic bacteria from yellow perch fillets. | studies on the generic identification of bacteria isolated from nonirradiated and irradiated (0.3 and 0.6 mrad) yellow perch fillets during the course of microbial spoilage have been conducted. after the enumeration and tabulation of macrocolonies on petri dish cultures obtained from fillets, isolates were examined and keyed out essentially according to modified morphological and biochemical protocols of shewan. identification was further confirmed through reference to bergey's manual. data obta ... | 1966 | 16349702 |
| pseudomonads and achromobacters in the spoilage of irradiated haddock of different preirradiation quality. | the effect of initial quality of fish on postirradiation (100 krad) changes in the bacterial flora of haddock fillets during aerobic storage at 3 c has been investigated, with emphasis on the pseudomonas and achromobacter groups. the quality was related to the length of time the eviscerated fish had been stored in ice prior to filleting. increased numbers of organisms, in particular pseudomonas putrefaciens, were found initially on fillets cut from older fish. pseudomonads were reduced by 2 to 3 ... | 1970 | 16349884 |
| growth in stewart's medium is a simple, rapid and inexpensive screening tool for the identification of burkholderia cepacia complex. | ninety-one percent of burkholderia cepacia complex reference strains (66 out of 72) displayed a yellow slope-green butt colour reaction after growth in stewart's medium indicating the oxidation of glucose and the absence of an arginine dihydrolase system. this same colour reaction was observed for burkholderia gladioli and several ralstonia species, but not for pseudomonas aeruginosa, stenotrophomonas, achromobacter, pandoraea and several other gram-negative non-fermenting bacilli. we therefore ... | 2006 | 16386966 |
| cleavage with phospholipase of the lipid anchor in the cell adhesion molecule, csa, from dictyostelium discoideum. | a cell adhesion molecule, 80-kda csa, is involved in edta-resistant cell contact at the aggregation stage of dictyostelium discoideum. a 31-kda csa was isolated from the 80-kda csa by treatment with achromobacter protease i. results from thin-layer chromatography and maldi-tof ms analysis indicated that the 31-kda csa contains ceramide as a component of glycosylphosphatidyl-inositol (gpi). comparison between the 80-kda csa and the 31-kda csa treated with phosphatidylinositol-specific phospholipa ... | 2006 | 16388974 |
| crystallization and preliminary x-ray crystallographic analysis of pas factor from vibrio vulnificus. | plasmid achromobacter secretion (pas) factor is a putative secretion factor that induces the secretion of periplasmic proteins. pas factor from vibrio vulnificus was crystallized at 294 k by the hanging drop vapor-diffusion method. it was isolated as a monomer during the purification procedures. the native crystal belongs to the f222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 a, a=b=g=90 degrees. the crystal was soaked in cryoprotectant containing 1 m nabr for 1 h for mad phasi ... | 2005 | 16404150 |
| molecular detection of catabolic genes for polycyclic aromatic hydrocarbons in the reed rhizosphere of sunchon bay. | this study focused on detecting catabolic genes for polycyclic aromatic hydrocarbons (pahs) distributed in the reed rhizosphere of sunchon bay, korea. these marsh and mud environments were severely affected by human activities, including agriculture and fisheries. our previous study on microbial roles in natural decontamination displayed the possibility that pah-degrading bacteria, such as achromobacter sp., alcaligenes sp., burkholderia sp. and pseudomonas sp. play an important decontamination ... | 2005 | 16410776 |
| [study on biodiversity of type i & ii polyketide synthesis genes positive microorganisms]. | some soil samples were collected from different places in yunnan provinces, china. 876 bacteria or actinomycete strains were isolated using glucose-peptone-yeast extract agar, starch-casein agar and glycerol-asparagine agar with these soil samples, of which about 100 strains belong to genus streptomyces and the others belong to rare actinomycetes or bacteria. with polyketide synthesis gene screening, 75 strains were picked up as type i & ii polyketide synthesis gene positive strains. then 10 str ... | 2005 | 16496684 |
| assessing the impact of denitrifier-produced nitric oxide on other bacteria. | a series of experiments was undertaken to learn more about the impact on other bacteria of nitric oxide (no) produced during denitrification. the denitrifier rhodobacter sphaeroides 2.4.3 was chosen as a denitrifier for these experiments. to learn more about no production by this bacterium, no levels during denitrification were measured by using differential mass spectrometry. this revealed that no levels produced during nitrate respiration by this bacterium were in the low mum range. this conce ... | 2006 | 16517672 |
| impact of a genetically engineered bacterium with enhanced alkaline phosphatase activity on marine phytoplankton communities. | an indigenous marine achromobacter sp. was isolated from coastal georgia seawater and modified in the laboratory by introduction of a plasmid with a phoa hybrid gene that directed constitutive overproduction of alkaline phosphatase. the effects of this "indigenous" genetically engineered microorganism (gem) on phosphorus cycling were determined in seawater microcosms following the addition of a model dissolved organic phosphorus compound, glycerol 3-phosphate, at a concentration of 1 or 10 (mu)m ... | 1996 | 16535222 |
| dissimilation of methionine by achromobacter starkeyi. | methionine was decomposed by some bacteria which were isolated from soil. the sulfur of the methionine was liberated as methanethiol, and part of this became oxidized to dimethyl disulfide. detailed studies with one of these cultures, achromobacter starkeyi, indicated that the first step in methionine decomposition was its oxidadative deamination to alpha-keto-gamma-methyl mercaptobutyrate by a constitutive amino acid oxidase. the following steps were carried out by inducible enzymes, the synthe ... | 1970 | 16559105 |
| bacterial oxidation of dipicolinic acid i. : isolation of microorganisms, their culture conditions, and end products. | arima, kei (university of tokyo, tokyo, japan) and yasuo kobayashi. bacterial oxidation of dipicolinic acid. i. isolation of microorganisms, their culture conditions, and end products. j. bacteriol. 84:759-764. 1962.-in a study of the metabolic pathway(s) of dipicolinic acid (dpa) in microorganisms, 436 strains of soil microorganisms were isolated by use of an enrichment culture technique. most of them were bacteria, and one of them, achromobacter, which had the strongest dpa-oxidizing activity, ... | 1962 | 16561964 |
| cyanide resistance in achromobacter i. induced formation of cytochrome a(2) and its role in cyanide-resistant respiration. | arima, kei (university of tokyo, tokyo, japan), and tetuo oka. cyanide resistance in achromobacter. i. induced formation of cytochrome a(2) and its role in cyanide-resistant respiration. j. bacteriol. 90:734-743. 1965.-by following the cytochrome concentrations during the growth cycle and under various conditions (aerobic, aerobic plus kcn, reduced aeration, anaerobic plus nano(3)) in achromobacter strain d, a close relationship between the formation of cytochrome a(2) (and a(1)) and the difficu ... | 1965 | 16562075 |
| cyanide resistance in achromobacter ii. mechanism of cyanide resistance. | oka, tetuo (university of tokyo, tokyo, japan), and kei arima. cyanide resistance in achromobacter. ii. mechanism of cyanide resistance. j. bacteriol. 90:744-747. 1965.-photochemical data showed that the only oxidase found in the cyanide-sensitive cells of achromobacter was cytochrome o, and that cyanide-resistant cells contained at least two oxidases. the oxidase responsible for cyanide resistance was a pigment the co compound of which had its absorption band at a wavelength longer than 580 mmu ... | 1965 | 16562076 |
| achromobacter ichthyodermis, n. sp., the etiological agent of an infectious dermatitis of certain marine fishes. | | 1934 | 16587855 |
| transient performance of two-phase partitioning bioreactors treating a toluene contaminated gas stream. | two-phase partitioning bioreactors (tppbs) consist of a cell-containing aqueous phase and an immiscible organic phase that sequesters and delivers toxic substrates to cells based on equilibrium partitioning. the immiscible organic phase, which acts as a buffer for inhibitory substrate loadings, makes it possible for tppbs to handle high volatile organic compound (voc) loadings, and in this study the performance of liquid n-hexadecane and solid styrene butadiene (sb) polymer beads used as partiti ... | 2006 | 16607657 |
| effects of microbial and phosphate amendments on the bioavailability of lead (pb) in shooting range soil. | | 2006 | 16652251 |
| isolation and characterization of two new microbial strains capable of degradation of the naturally occurring organophosphonate - ciliatine. | air-born mixed fungal and bacterial culture capable of complete degradation of ciliatine was isolated. the utilization of the natural organophosphonate proceeded in the phosphate independent manner. enzymatic activity involved in ciliatine degradation studied in the fungal cell-free extract proved to be distinct from bacterial pathway described before. | 2007 | 16758270 |
| chronic infection with achromobacter xylosoxidans in cystic fibrosis patients; a retrospective case control study. | in cystic fibrosis (cf), chronic infection of the airways with achromobacter xylosoxidans have become more frequent. the pathogenic role of this is yet unclear. | 2006 | 16777495 |
| analysis of denitrification genes and comparison of nosz, cnorb and 16s rdna from culturable denitrifying bacteria in potato cropping systems. | bacterial denitrification in agricultural soils is a major source of nitrous oxide, a potent greenhouse gas. this study examined the culturable bacterial population of denitrifiers in arable field soils in potato (solanum tuberosum l.) production and denitrification genes (nir, nor and nos) and 16s rdna in those isolates. enrichments for culturable denitrifiers yielded 31 diverse isolates that were then analysed for denitrification genes. the nitrous oxide reductase (nosz) gene was found in all ... | 2007 | 16793234 |
| achromobacter xylosoxidans in cystic fibrosis: prevalence and clinical relevance. | achromobacter xylosoxidans is increasingly cultured in sputum from cystic fibrosis (cf) patients; nevertheless, there are few published data on the clinical impact of this infection or chronic colonisation. | 2007 | 16793350 |
| active site comparison of coii blue and green nitrite reductases. | copper-containing nitrite reductases (nirs) possess type 1 (t1) and type 2 (t2) copper sites and can be either green or blue in color owing to differences at their t1 centers. the active sites of a green and a blue nir were studied by utilizing their t1cui/t2coii and t1coii/t2coii-substituted forms. the uv/vis spectra of these derivatives highlight the similarity of the t2 centers in these enzymes and that t1 site differences are also present in the coii forms. the paramagnetic nmr spectra of t1 ... | 2006 | 16795108 |
| direct estimation of the oxygen requirements of achromobacter xylosoxidans for aerobic degradation of monoaromatic hydrocarbons (btex) in a bioscrubber. | the o2 requirements for biomass production and supplying maintenance energy demands during the degradation of both benzene and ethylbenzene by achromobacter xylosoxidans y234 were measured using a newly proposed technique involving a bioscrubber. using this approach, relevant microbial parameter estimates were directly and simultaneously obtained via linear regression of pseudo steady-state data. for benzene and ethylbenzene, the biomass yield on o2, y(x/o2), was estimated on a cell dry weight ( ... | 2006 | 16802093 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| effectiveness of biodegradation of petroleum products by mixed bacterial populations in liquid medium at different ph values. | the possibility of using mineral oils as a carbon source by bacteria adapted to high oil concentrations was tested in liquid media with different ph values (ph = 5, 7 and 9). two types of inocula were tested: inoculum i consisted of selected strains used in the bioremediation of oil-contaminated soils and inoculum ii contained bacteria isolated from soil samples previously bioremediated at ph = 5, 7 and 9. biodegradation was observed in all the investigated media independently of initial ph valu ... | 2006 | 16878607 |
| insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from achromobacter cycloclastes. | the difficult chemistry of nitrous oxide (n2o) reduction to gaseous nitrogen (n2) in biology is catalysed by the novel micro4-sulphide-bridged tetranuclear cuz cluster of the n2o reductases (n2or). two spectroscopically distinct forms of this cluster have been identified as cuz and cuz*. we have obtained a 1.86 a resolution crystal structure of the pink-purple species of n2or from achromobacter cycloclastes (acn2or) isolated under aerobic conditions. this structure reveals a previously unobserve ... | 2006 | 16904686 |
| benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation. | the performance and stability of a two-phase partitioning bioscrubber (tppb) containing 33% (vol.) n-hexadecane as an immiscible phase was investigated during 30 days of continuous gaseous benzene treatment. elimination capacities of 141 +/- 12 g/m(3) h were achieved by achromobacter xylosoxidans y234 while maintaining >99% removal throughout. a new steady-state operating strategy that limits excessive biomass production by directing substrate consumption to maintenance energy has eliminated the ... | 2006 | 16941180 |
| biotransformation of isoeugenol to vanillin by pseudomonas putida ie27 cells. | the ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. the vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera achromobacter, aeromonas, agrobacerium, alcaligenes, arthrobacter, bacillus, micrococcus, pseudomonas, rhodobacter, and rhodococcus. strain ie27, a soil isolate showing the highest vanillin-producing activity, was identified as pseudomonas putida. we optimized the culture ... | 2007 | 16944125 |
| fluorescence in situ hybridization for rapid identification of achromobacter xylosoxidans and alcaligenes faecalis recovered from cystic fibrosis patients. | achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (cf) patients, but identification with biochemical tests is unreliable. we describe fluorescence in situ hybridization assays for the rapid identification of achromobacter xylosoxidans and alcaligenes faecalis. both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from cf patients. | 2006 | 16954289 |
| plant growth promoting potential of free-living diazotrophs and other rhizobacteria isolated from northern indian soil. | the viable count of free-living diazotrophic bacteria in different crop rhizospheres varied from 1.11 x 10(4) to 8.5 x 10(5) cfu/g of soil. the majority of the diazotrophs phenotypically belong to either azotobacter chroococcum, non-a. chroococcum type and to a heterogenous group tentatively named putative nitrogen-fixing (pnf) bacteria. in this study, 25 isolates of the pnf group were screened for their multiple plant growth-promoting (pgp) traits and grouped into 5 pgp types. an isolate, pnf(1 ... | 2006 | 17004301 |
| ciprofloxacin-resistant gram-negative bacilli in the fecal microflora of children. | the extent to which antibiotic-resistant bacteria are excreted by humans who have not been exposed to antibiotics is not known. children, who rarely receive fluoroquinolones, provide opportunities to assess the frequency of fecal excretion by fluoroquinolone-naïve hosts of fluoroquinolone-resistant gram-negative bacilli. fresh nondiarrheal stools from children were processed by screening them on agar containing ciprofloxacin to recover ciprofloxacin-resistant gram-negative bacilli. resistant iso ... | 2006 | 17005812 |
| structural studies of apo nosl, an accessory protein of the nitrous oxide reductase system: insights from structural homology with merb, a mercury resistance protein. | the formation of the unique catalytic tetranuclear copper cluster (cu(z)) of nitrous oxide reductase, n(2)or, requires the coexpression of a multiprotein assembly apparatus encoded by the nosdfyl operon. nosl, one of the proteins encoded by this transcript, is a 20 kda lipoprotein of the periplasm that has been shown to bind copper(i), although its function has yet to be detemined. cu(i) exafs data collected on the holo protein demonstrated that features of the copper binding site are consistent ... | 2006 | 17014077 |
| construction of a leftover bath water model for microbial testing. | in this study, in order to construct a model of leftover bath water, we analyzed one hundred samples of used bath water samples which were provided by twenty-eight volunteer families. it appeared that the number of detected bacteria from such bath water was correlated closely with the number of bathers. moreover, the ph, acidity, chemical oxygen demand (cod), ion, protein content of the leftover bath water were measured. the number of bathers had no connection with the ph, acidity, cod, and ion ... | 2006 | 17017128 |
| analysis of the composition of bacterial communities in oil reservoirs from a southern offshore brazilian basin. | the aim of this study was to characterize and compare the bacterial community structure of two distinct oil samples from a petroleum field in brazil by using both molecular, based on the construction of 16s rrna gene libraries, and cultivation methods. statistical comparisons of libraries based on amplified ribosomal dna restriction analysis (ardra) data revealed no significant differences between the communities recovered in the non-biodegraded (nbd) and highly biodegraded oils (hbd). blastn an ... | 2007 | 17072536 |
| bilateral simultaneous achromobacter xylosoxidans keratitis following penetrating keratoplasty. | we report a case of severe bilateral simultaneous achromobacter xylosoxidans keratitis following penetrating keratoplasty (pkp) in a patient with graft-versus-host disease. the predisposing factors, clinical features, treatment options, and final visual outcome are reviewed. to our knowledge, this is the first report of bilateral a xylosoxidans keratitis following pkp. | 2006 | 17138001 |
| correlation of functional instability and community dynamics in denitrifying dispersed-growth reactors. | understanding the relationship between microbial community dynamics and functional instability is an important step towards designing reliable biological water treatment systems. in this study, the community dynamics of two dispersed-growth denitrifying reactors were examined during periods of functional stability and instability. in both reactors during the period of functional instability, the effluent chemistry changed over time, with periods of high nitrate concentrations followed by periods ... | 2007 | 17142382 |
| pannonibacter phragmitetus, described from a hungarian soda lake in 2003, had been recognized several decades earlier from human blood cultures as achromobacter groups b and e. | we performed a polyphasic taxonomic study on isolates previously tentatively classified as achromobacter groups b and e in comparison with the type strain of pannonibacter phragmitetus, lmg 22736(t)=nctc 13350(t). comparative 16s rrna gene sequence analysis suggested that strains of achromobacter groups b and e belong to p. phragmitetus (similarity levels were higher than 99 %). dna-dna hybridization experiments and other genotypic and phenotypic analyses confirmed that the three taxa represent ... | 2006 | 17159003 |
| infection of a total hip prosthesis in a dog caused by achromobacter(alcaligenes) xylosoxidans. | a four-year-old male, neutered labrador retriever was presented with progressive left hindlimb lameness 10 months following total hip replacement. radiography revealed changes consistent with infection and culture of joint fluid from the left coxofemoral joint revealed achromobacter (alcaligenes) xylosoxidans. the prosthesis was removed. culture of the acetabular cup confirmed achromobacter xylosoxidans. achromobacter xylosoxidans is an uncommon but serious cause of nosocomial epidemics in hospi ... | 2006 | 17201829 |