Publications

TitleAbstractYear
Filter
PMID
Filter
antibody response in patients infected with one common strain of campylobacter jejuni. evidence for production of antibodies against thermostable soluble antigens.antibody production was investigated for ten patients from a group of 20 who had contracted infections with a strain of campylobacter jejuni of serotype pen 6,7. production of antibody was determined by titrating ten sets of paired sera for agglutination of formalin-treated and heat-treated cell suspensions and by passive hemagglutination using both sheep and human o rh-red blood cells sensitized with extracted soluble thermostable antigens. all patients had demonstrable antibodies against the f ...19863528928
[experience in isolating campylobacter jejuni from patients with acute intestinal diseases].erythrite agar, a commercial culture medium produced by the research institute for culture media (makhachkala, ussr), is suitable for the cultivation of c. jejuni after the addition of hemolized sheep blood. the addition of antibiotics to the medium permits its use for the isolation of c. jejuni from feces. by means of this medium c. jejuni has been isolated from the feces of 10% of patients hospitalized on account of acute infectious intestinal diseases.19863529758
[epidemiologic evaluation of a sample of 91 rats (rattus norvegicus) captured in the sewers of lyon].91 rats captured in 1982 in the sewers of lyon (france) have been examined for the presence of some microorganisms implicated in infections transmissible to man, by direct examination, bacteriological culture or detection of specific antibodies. bacteriological results have shown a high proportion of carriers of yersinia enterocolitica and frederiksenii (29%), of pasteurella pneumotropica (29%), of staphylococcus aureus (53%), and of campylobacter jejuni (18%), a low proportion of carriers of sa ...19863532637
direct immunofluorescence microscopy for rapid screening of campylobacter enteritis.diagnostic use of a direct fluorescent-antibody test for detection of campylobacter jejuni and c. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). conjugates were prepared from immunoglobulin g antibody against 22 lior c. jejuni and c. coli reference strains (h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). interestingly, the serotypes of cultures tested by the direct f ...19863533990
[campylobacter jejuni/coli--isolated by coprocultures from cases of acute diarrhea]. 19863538336
[incidence and transmission of campylobacter jejuni/coli in the production of young fattening poultry. 2. study of the characterization, resistance behavior and pathogenicity of campylobacter jejuni/coli in poultry]. 19863542476
[comparison of various selective supplements in the cultivation of campylobacter jejuni/coli from fecal samples]. 19863544537
survey of campylobacter jejuni and enterotoxigenic escherichia coli in kenya. 19863546042
the comparative activity of pefloxacin, enoxacin, ciprofloxacin and 13 other antimicrobial agents against enteropathogenic microorganisms.in this study, we compared the activity of pefloxacin, enoxacin and ciprofloxacin against 269 enteropathogenic strains (campylobacter jejuni, enteropathogenic escherichia coli, salmonella typhi, shigella spp., vibrio cholerae and yersinia enterocolitica) with that of rosoxacin, flumequin, nifuroxazide, erythromycin, chloramphenicol, ampicillin, cefotaxime, tetracycline, amikacin, netilmicin, sulfamethoxazole, trimethoprim and co-trimoxazole. pefloxacin, enoxacin and ciprofloxacin were always amo ...19863546145
gastrointestinal zoonoses.infectious gastrointestinal diseases affect man and animals throughout the world. certain etiologic agents (for example, salmonella spp., campylobacter jejuni, yersinia enterocolitica, cryptosporidia, strongyloides stercoralis, echinococcus granulosa) seem to have the potential to be transmitted from pets to people, causing severe disease in the latter. other agents seem unlikely to be transmitted but may have the potential to be zoonoses. this article discusses proved, suspected, and possible z ...19873551302
susceptibility of campylobacter jejuni and yersinia enterocolitica to uv radiation.two enteric pathogens, campylobacter jejuni and yersinia enterocolitica serogroup o:3, together with escherichia coli, were investigated for susceptibility to uv radiation at 254 nm. the uv dose required for a 3-log reduction (99.9% inactivation) of c. jejuni, y. enterocolitica, and e. coli was 1.8, 2.7, and 5.0 mws/cm2, respectively. using e. coli as the basis for comparison, it appears that c. jejuni and y. enterocolitica serogroup o:3 are more sensitive to uv than many of the pathogens associ ...19873551844
methods for detecting and enumerating campylobacter jejuni and campylobacter coli in poultry.methods, media, and biochemical tests for detecting, enumerating, isolating, and identifying campylobacter jejuni and c. coli in foods are summarized with special consideration of poultry and poultry products. information is drawn largely from the american public health association compendium of methods for the microbiological examination of foods and the us food and drug administration bacteriological analytical manual for foods. reference is also made to recently advanced techniques and proced ...19863554203
[infectious diarrhea in children. results of a prospective study concerning various epidemiological and clinical aspects of infectious diarrhea in children].over a 3-year period (from october 1981 to september 1984) 352 pediatric patients were treated for infectious diarrhea at the division for pediatric infectious diseases, department of pediatrics, university of berne, switzerland. routine diagnostic tests on stool samples included cultures for salmonella, shigella, campylobacter and yersinia, and enzyme-linked immunosorbent assay (rotazyme) for rotaviruses. in selected cases special tests for parasitic pathogens were also performed. the patients ...19873554501
current problems in antimicrobial therapy for bacterial enteric infection.trimethoprim/sulfamethoxazole is currently considered the treatment of choice for shigellosis and severe travelers' diarrhea. the problem with this combination regimen is inactivity against campylobacter jejuni strains and other bacterial enteropathogens showing in vitro resistance to the drug. resistance to trimethoprim/sulfamethoxazole among enteric pathogens has occurred frequently in certain areas of the world. a study of the in vitro susceptibility of enteric bacterial pathogens isolated fr ...19873555056
clinical efficacy of ciprofloxacin compared with placebo in bacterial diarrhea.in a double-blind, randomized trial, 85 adult patients with acute diarrhea (more than three watery stools per day) received either 500 mg of ciprofloxacin twice daily or placebo for five days. seventy-six patients were evaluated, 38 patients in the ciprofloxacin group (16 with salmonella species, 19 with campylobacter jejuni, and three with shigella species) and 38 patients in the placebo group (21 with salmonella species, 11 with c. jejuni, and six with shigella species). the duration of fever ...19873555057
[comparison of campylobacter jejuni isolation methods and the effect of moisture content on colony morphology].several isolation methods and media and the effect of moisture content on colony morphology were compared for the primary isolation of campylobacter jejuni. of the 200 rectal swabs from cattle and sheep tested for the isolation of c. jejuni, 19.5% were positive on butzler medium and 16.5% were positive on skirrow medium. the transportation of samples in modified cary-blair medium increased the isolation rate. of the 300 gallbladder from cattle and sheep tested for the isolation of c. jejuni, 5% ...19863561277
[the incidence of campylobacter jejuni and other pathogenic bacteria in childhood summer diarrhea].it was investigated the incidence of campylobacter jejuni which has been recognized as a common cause of akut gastroenteritis and other pathogenic bacteria in childhood summer diarrhea. it was examined 94 diarrheic patients stool in 0-5 years old in summer months 1985-86. campylobacter jejuni, salmonella, shigella were isolated as a pathogenic bacteria.19863561278
[a waterborne outbreak of campylobacter jejuni infection]. 19873563986
[experimental animal studies with campylobacter jejuni and campylobacter coli in chickens]. 19873566434
a study of factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacter jejuni and campylobacter coli and recommendations for a more rapid procedure.factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacters have been studied. the concentration of red blood cells during the haemagglutination stage of the procedure markedly affected the titer obtained. an increase in concentration of red blood cells resulted in a lower titer, with titers being inversely proportional to red blood cell concentration. no differences in titer were observed when erythrocytes were sensitized at a range of ph values betwe ...19873567742
biotypes and serotypes among campylobacter jejuni strains in lagos. 19863568121
use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by streptococcus mutans.local passive immunization with monoclonal antibodies (mabs) raised against streptococcal antigen (sa) i/ii protects monkeys against colonization of teeth by streptococcus mutans and the subsequent development of dental caries. in this study we extended the preclinical experiments to human subjects. in the first study of eight healthy subjects, four had anti-sa i/ii mab (immunoglobulin g2a [igg2a]) and four had saline applied to their teeth on three occasions. a streptomycin-resistant s. mutans ...19873570463
intestinal mucus gel and secretory antibody are barriers to campylobacter jejuni adherence to int 407 cells.an in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin a (siga) in preventing attachment of campylobacter jejuni to int 407 cells. an overlay of rabbit small intestinal mucus was found to impede the attachment of c. jejuni to a monolayer of int 407 cells. mucus from rabbits previously colonized with c. jejuni was found to completely inhibit bacterial adherence to the underlying cells. anti-campylobacter siga was readily detected in mucus samples from pr ...19873570473
effect of medium supplements, illumination and superoxide dismutase on the production of coccoid forms of campylobacter jejuni atcc 29428.factors influencing the production of coccoid forms in cultures and suspensions of a strain of the enteric pathogen campylobacter jejuni during storage in air were investigated. addition of blood or a supplement containing ferrous sulphate, sodium metabisulphite and sodium pyruvate minimized conversion of rods to coccoid forms in cultures. exposure of cultures to light during storage in air increased the rate of production of coccoid forms. ultraviolet radiation was shown to effect the viability ...19873571033
a study of thermophilic campylobacters in a river system.fifteen kilometres of a river system traversing rural and urban areas and subject to sewage works effluent discharge was studied during a 12 1/2 month period. a total of 312 samples was collected from 12 sites at 14 d intervals and tested by a glass microfibre filtration method and a most probable number (mpn) method. campylobacters were found in 43% of samples by the filtration method and 21% by the mpn method. the lowest frequency of isolation and lowest counts (less than 10 campylobacters/100 ...19873571039
differentiation of campylobacter and campylobacter-like organisms by cellular fatty acid composition.the cellular fatty acid compositions of 368 strains of campylobacter species or campylobacter-like organisms were determined by gas-liquid chromatography. most of the strains (339) were placed in one of three groups based on differences in fatty acid profiles. group a contained campylobacter jejuni (97%) and most c. coli (83%) strains and was characterized by the presence of a 19-carbon cyclopropane fatty acid (19:0 cyc) and 3-hydroxytetradecanoic acid (3-oh-14:0). group b included all c. laridi ...19873571478
isolation of campylobacter jejuni from diarrhoea patients in eastern province of saudi arabia. 19863571879
anterior uveitis associated with campylobacter jejuni infection. 19873572019
prevalence of campylobacter jejuni and campylobacter coli among wild and domestic animals in yamaguchi prefecture. 19863574158
campylobacter enteritis in childhood.bacteriological examinations of faecal samples, obtained from 321 infants and children with acute enteritis, were carried out in the pediatric clinic of the university of occupational and environmental health, japan from january 1983 to december 1985. campylobacter jejuni were isolated in 48 infants and children (15%), while salmonella species in 6 (1.9%), and enteropathogenic escherichia coli in 11 (3.4%). of 48 infants and children with campylobacter enteritis (c. enteritis), 20 cases (42%) we ...19873576011
decontamination of campylobacter jejuni on chicken drumsticks using chemicals and radiation.a trial was conducted to compare the efficacy of four disinfectants and radiation to reduce the level of c. jejuni contamination on poultry meat. two levels of each treatment were applied to chicken drumsticks inoculated with a known concentration of a strain of campylobacter jejuni, biotype 1, isolated from a human patient with diarrhea. radiation using a cobalt-60 source at a level of 0.5 kgy effected a 99% surface reduction in c. jejuni. with a mean initial surface contamination level of 1.1 ...19873576968
[studies on phage typing of campylobacter jejuni/coli]. 19863577114
diarrheal morbidity and mortality in children in the central african republic.diarrheal morbidity and mortality in children less than 5 years old were studied in bangui, central african republic, by a cluster survey. we found a high prevalence of diarrheal disease with an estimated annual incidence of 7 episodes of diarrhea per child per year. the estimated annual mortality rate for children less than 5 years old was 28.6 per 1,000 and 85.8 per 1,000 for infants; 51.6% of deaths were reported to be associated with diarrhea. during the survey, stool specimens were collecte ...19873578657
erythromycin-resistant campylobacter infections in thailand.erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ...19873579261
comparative in vitro antibacterial activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074).the in vitro activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074), were tested against 990 clinical bacterial isolates in comparison with that of cephalexin. both compounds were more active than cephalexin against gram-negative bacteria, inhibiting most isolates of the family enterobacteriaceae at concentrations of less than or equal to 4 micrograms/ml, but were not active against acinetobacter species, most pseudomonas species, campylobacter jejuni, and ...19873579264
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
[campylobacter jejuni infection in children]. 19873580099
gastric lavage: a simple method to obtain iga-rich intestinal secretions from the rabbit.a lavage procedure was developed to obtain intestinal secretions from rabbits. the procedure facilitated the repeated monitoring of the intestinal iga immune response of these animals to enteric infection with campylobacter jejuni. this non-invasive technique was easily performed, reproducible and yielded consistent levels of iga from rabbit intestinal secretions. it is anticipated that this procedure will aid in the study of the intestinal immune response of rabbits to other enteric pathogens.19873584997
in-vitro and in-vivo studies of a cytotoxin from campylobacter jejuni.studies were performed on a cytotoxin (ct) from human strains of campylobacter jejuni isolated in malaysia. ct was detected by cytopathic effect (cpe) on hela cells at titres from 8 to 32, in culture filtrates from 14 (48%) of 29 human isolates. the cpe correlated well with a quantitative 51cr-release assay where a specific release of 54-68% was noted. ct production was lost after 5-7 subcultures. ct activity was also detected in 5 (26%) of 19 faecal filtrates from which ct-producing isolates we ...19873585956
the mechanism of protection of infant mice from intestinal colonisation with campylobacter jejuni.balb/c mice, vaccinated intraperitoneally with a heat-killed (62 degrees c) suspension of campylobacter jejuni before mating, completely protect c. 90% of their own infants from intestinal colonisation. this protection has now been investigated further in fostering experiments. fostering by vaccinated dams within the first 24 h of life prevented intestinal colonisation in 50% of infants from non-vaccinated dams, and reduced colonisation in a further 25%. infants from vaccinated dams, even if all ...19873585966
pediatric gastroenteritis in primary care and in hospitalized patients.one-hundred and fifty-seven consecutive children below seven years of age (primary care n = 48, hospitalized patients n = 109) with acute gastroenteritis of assumed infectious origin were studied. rotavirus was demonstrated by electron microscopy of faeces in 44% of all patients. the occurrence of rotavirus among patients in primary care, 15%, was significantly lower than among hospitalized patients, 57% (p less than 0.01). adenovirus was isolated in six per cent and enterovirus in two per cent ...19873589234
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
clinical impact of stool cultures for campylobacter in adults with acute or chronic diarrhea.campylobacter jejuni has emerged as a frequent cause of diarrhea. during a 12-month period at the houston veterans administration medical center, we isolated c jejuni from 3.4% of the 290 stool cultures from patients with diarrhea. this compared to an isolation rate of 4.1% for salmonella and 3.1% for shigella. during the same period, 17 additional cases of campylobacter-associated diarrhea were identified in adults at the two other baylor college of medicine teaching hospitals. we correlated th ...19873589763
serum agglutinin titers against somatic and flagellar antigens of campylobacter jejuni and isolation of campylobacter spp in chickens.from june 1983 to june 1984, two hundred twenty-five 3- to 30-month-old chickens (hens) on 10 different farms were examined for campylobacter spp. watering trays and fly vectors also were examined for campylobacter spp on 6 of the 10 farms. campylobacter jejuni was isolated from fecal specimens from 64 hens (28.4%), c coli was isolated from 6 hens (2.7%), and c laridis was isolated from 9 hens (4%). the isolation rate of c jejuni was 6.7% to 46.7% for 9 of the 10 farms. on 2 farms, agglutinin ti ...19873592379
comparative in vitro activity of the new oral cephalosporin bmy-28100.using a broth microdilution method, the in vitro activity of bmy-28100 against 365 clinical strains of commonly isolated bacteria was determined. bmy-28100 showed good activity against streptococci, methicillin-susceptible staphylococci, salmonella spp., shigella spp., and beta-lactamase producing branhamella catarrhalis and haemophilus influenzae. against susceptible strains of these organisms, bmy-28100 showed activity comparable to that of penicillin g, ampicillin, co-trimoxazole, erythromyci ...19873595580
a blood-free medium for isolation of thermophilic campylobacter species.a blood-free medium for the recovery of thermophilic campylobacter was compared with butzler medium virion during a one-year study using 2,893 human feces samples. ninety campylobacter strains (3.1%) were isolated after incubation for 48 h at 42 degrees c in a candle jar atmosphere. three strains of campylobacter jejuni were isolated on the blood-free medium only and one on butzler medium virion only. fecal flora was equally well inhibited on both media except for gram-positive organisms, which ...19873595583
campylobacter jejuni in dairy cows and raw milk.twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ...19873595744
efficacy of some methods and media for detecting and enumerating campylobacter jejuni in frozen chicken meat.four of five strains of campylobacter jejuni survived in chicken meat stored at -18 degrees c for 12 months. direct plating of samples was superior to the most probable number technique for enumerating c. jejuni. enrichment culture using the doyle & roman enrichment method resulted in the highest rates of detection. packaging under an atmosphere of co2 did not substantially influence survival.19873597202
toxic megacolon due to campylobacter colitis.campylobacter jejuni is a common cause of acute infectious diarrhoea. most patients recover in less than a week, but 20 per cent may have a prolonged or severe illness [1]. we describe a patient who developed a fulminant toxic dilatation of the colon necessitating a subtotal colectomy.19863598315
erythromycin for campylobacter jejuni-associated dysentery. 19873598790
occurrence of campylobacter jejuni in dog faeces from the streets of a southern chilean city. 19863602827
campylobacter jejuni enteritis associated with raw goat's milk.during a three-week period in july 1983, six cases of campylobacter jejuni enteritis in king county, washington were associated with a dairy that produced raw goat's milk. four patients consumed the dairy's milk, and the other two patients comprised an employee of the dairy and her infant son. two case-control studies confirmed that, at the time the cases occurred, consumption of the dairy's milk was a risk factor for c. jejuni enteritis in king county. c. jejuni was isolated from the intestinal ...19873605046
isolation of campylobacter spp. from pigeon feces by a combined enrichment-filtration technique.a technique combining enrichment in preston enrichment broth and direct filtration onto chocolate agar was used to isolate campylobacter species from pigeon feces. campylobacter jejuni was isolated from 106 of 200 samples tested; 105 strains were isolated by enrichment-filtration, and 84 strains were isolated by direct plating. most of the strains grew after 48 h at 37 degrees c.19873606114
minicell formation by campylobacter jejuni.campylobacter jejuni sheds its flagella and varying proportions of the poles of the cell late in the growth cycle, resulting in the production of very small flagellated structures 0.1 to 0.3 microm in diameter. electron microscopy revealed that these structures were minicells possessing outer membrane, cytoplasmic membrane, flagellar basal complex, and polar membrane; nucleoplasms were not seen. the initial event in the formation of these minicells involved a constriction of the cytoplasmic memb ...19873607612
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
kinetics of anti-campylobacter jejuni monomeric and polymeric immunoglobulin a1 and a2 responses in serum during acute enteritis.the intensity and kinetics of the serum polymeric and monomeric immunoglobulin a1 (iga1) and iga2 antibody responses to campylobacter jejuni were analyzed. a rapid and marked serum iga antibody response involving both the monomeric and polymeric components of iga was observed after c. jejuni infections. iga antibodies reached a peak of activity in serum during week 2 after the first symptoms of enteritis, about 10 days before the peak of igg activity. polymeric iga accounted for most of the anti ...19873611318
[pharmacokinetic and clinical studies of cefuzonam in pediatrics].pharmacokinetic and clinical studies were conducted to evaluate cefuzonam (l-105, czon), a new cephem type antibiotic, in the pediatric field. a total of 9 pediatric patients (2-14 years) was treated with intravenous injection of czon: 4 cases with one shot of 20 mg/kg, 2 cases with one shot of 40 mg/kg and 3 cases with drip infusion over 1 hour of 40 mg/kg. czon concentrations in serum and the excretion in urine were determined. mean serum concentrations of czon after one shot intravenous injec ...19873613084
[a serogrouping scheme for campylobacter jejuni by the slide agglutination method with formation-treated antigens]. 19873613099
[campylobacter jejuni in acute infantile diarrhea]. 19873616245
tubulointerstitial nephritis as a complication of campylobacter jejuni enteritis. a case report.tubulointerstitial nephritis occurred as a complication of campylobacter jejuni enteritis in a previously healthy young man. campylobacter jejuni was isolated from the patient's faeces and nephritis was confirmed by renal biopsy.19873616508
campylobacter enteritis at a university: transmission from eating chicken and from cats.campylobacter jejuni is the most common enteric pathogen isolated from university and college students in the united states. during the fall and winter quarters of the 1983-1984 academic year, the authors conducted a case-control study at the university of georgia, athens, georgia, to identify risk factors for c. jejuni enteritis. students with diarrhea whose cultures yielded c. jejuni were compared with controls matched by age, sex, and residence. a total of 45 case-control pairs were interview ...19873618583
effect of clavulanic acid on susceptibility of campylobacter jejuni and campylobacter coli to eight beta-lactam antibiotics.the effect of clavulanic acid on the susceptibility of 32 strains of campylobacter jejuni and campylobacter coli to eight beta-lactam agents was studied. almost all strains tested became susceptible to amoxicillin and ticarcillin with 1 microgram of clavulanic acid per ml. this compound had little or no effect on susceptibility to penicillin g, cephalothin, cefamandole, and cefoxitin. clavulanic acid had a marginal effect on cefotaxime and moxalactam susceptibility.19873619428
[an investigation of the campylobacter jejuni carrier state in healthy children of kindergartens]. 19873621322
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
factors affecting production of coccoid forms by campylobacter jejuni on solid media during incubation.conditions influencing the conversion of oxygen into toxic derivatives in media were investigated for their effects on production of coccoid forms in cultures of the enteric pathogen campylobacter jejuni. compared with stored media, production of coccoid forms was less on freshly prepared media. whether freshly prepared or stored before use, brucella agar media produced the fewest coccoid forms under the test conditions. addition of supplements used as detoxifying agents minimized production of ...19873624106
hepatic lesions in experimental campylobacter jejuni infection of mice.mice orally infected with campylobacter jejuni developed focal infiltrative necrotic lesions in the liver, as determined by both histology and liver function tests. the initial histopathological feature was a focal infiltrative lesion in the parenchyma and portal triads. foci of infiltrative lesions became necrotic between days 30 and 60 post-inoculation (p.i.). during this period, portal infiltrates increased in severity. from month 4 p.i., focal areas of infiltrative necrosis in the liver pare ...19863625168
campylobacter jejuni/campylobacter coli-associated guillain-barré syndrome. immunoblot confirmation of the serological response.immunoblot (western blot) examination of the sera of 45 patients with the guillain-barré syndrome demonstrated that between three and five immunoreactive bands that were characteristic of a recent campylobacter jejuni/campylobacter coli infection were present in 22 of these patients. the immunoblots paralleled the serological response that is found in campylobacter enteritis and confirmed the specificity of our previous enzyme-linked immunosorbent assays (elisas), which indicates that antecedent ...19873626926
enteric campylobacter infection in gnotobiotic calves and lambs.gnotobiotic calves and lambs were infected orally with campylobacter jejuni, c coli or c hyointestinalis to assess pathogenicity. all animals were successfully colonised and excreted mucoid faeces but showed no other clinical signs. campylobacters colonised the large intestine better than the small intestine, in which bacterial numbers decreased with time after infection. campylobacters were found occasionally in the lumen of crypts in close proximity to epithelial cells and included in a mucus- ...19873628988
[clinical characteristics of acute intestinal diseases caused by campylobacter jejuni]. 19873629390
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
predictive value of stool examination in acute diarrhea.we prospectively evaluate the value of fecal blood and fecal leukocytes in predicting whether acute diarrhea in adults is associated with a stool culture positive for a bacterial pathogen. one hundred thirteen patients, aged 19 to 50 years, seen in a two-year period in an urban adult outpatient setting underwent stool culture for the presenting symptom of diarrhea. heterosexual men represented 48% of the cohort, women represented 17%, and homosexual men represented 35%. overall, 53 (47%) of the ...19873632285
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...19873651881
production of a unique cytotoxin by campylobacter jejuni.campylobacter jejuni is an important diarrheal pathogen worldwide; the mechanisms by which it causes disease remain unclear. because of its association with inflammatory diarrhea, we postulated that c. jejuni might produce a cytotoxin similar to that produced by shigella sp., enterohemorrhagic escherichia coli o157, or clostridium difficile. filtrates of 12 polymyxin-treated isolates of c. jejuni were placed on hela cells (sensitive to shiga toxin cytotoxicity) and chinese hamster ovary (cho) ce ...19873653987
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
rapid identification of campylobacter pylori (c. pyloridis) by preformed enzymes.campylobacter pylori (c. pyloridis) is a fastidious organism found in the gastric mucosa associated with histological gastritis and peptic ulceration. a rapid identification scheme that detects the presence of preformed enzymes (rosco diagnostica, taastrup, denmark) was applied to clinical isolates of c. pylori. the isolates tested were a very homogeneous group. they all produced oxidase, catalase, urease, alkaline phosphatase, gamma-glutamyl aminopeptidase, leucine aminopeptidase, and dnase. no ...19873654941
prevalence and characterization of hippurate-negative campylobacter jejuni in king county, washington.a total of 593 strains of thermophilic campylobacter species were isolated either from humans with diarrhea or from poultry in king county, washington. of these strains, 98 (52 hippurate-positive strains and all 46 of the hippurate-negative strains) were selected for further phenotypic characterization and genetic classification. hippurate hydrolysis, the test typically used to differentiate campylobacter jejuni and c. coli, did not always correlate with the genetic classification. all hippurate ...19873654945
rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli.a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates.19873654950
problems in identification of campylobacter jejuni associated with acquisition of resistance to nalidixic acid.in a patient with campylobacteriosis, we observed development of resistance to nalidixic acid and norfloxacin under adequate treatment with the latter antibiotic. isolates before and after treatment differed in their total protein profile but were otherwise identical with respect to enzymatic activity, serotype, crude membrane protein profiles, and other phenotypic characteristics.19873654954
serological study of campylobacter jejuni infection in slaughterhouse workers. 19873655409
counterimmunoelectrophoresis compared with complement fixation and passive haemagglutination tests in the evaluation of the immune response in campylobacter infections.the immune response was studied in 238 human patients with campylobacter jejuni/coli (cjc)-infections in rotterdam by the counterimmunoelectrophoresis (cie) test, a commercial complement fixation test (cft) and the passive haemagglutination test (ha). antibodies became detectable in the three tests around 4 days after the onset of complaints. between the 7th and the 20th days after onset of illness 79%, 80% and 53% of the patients demonstrated antibodies by the cie, the cft and the ha, respectiv ...19873662488
effect of sub-inhibitory concentrations of antibiotics on adhesion of campylobacter jejuni in vitro.the effects of sub-inhibitory concentrations (mic 50 and mic 25) of clindamycin, erythromycin and chloramphenicol on the adherence of campylobacter jejuni to int 407 cells were studied. the results indicated that the adhesion was inhibited at various extents, mostly by mic 50, and that the inhibition increased with time. the interpretation of our data is that the antibiotics can interfere with the adhesin(s) synthesis.19873663336
[a study on the epidemiological features and serotype distribution of campylobacter jejuni enteritis in children]. 19873664621
campylobacter jejuni: a common cause of gastroenteritis in rural ireland. 19873667193
pathology of campylobacter jejuni abortion in sheep.campylobacter jejuni was inoculated intravenously into pregnant ewes on gestation days 114 and 123 to reproduce ovine abortion. all ewes aborted 7-12 days post-inoculation. high numbers of c. jejuni were isolated from ewe tissues (caruncle, bile, cecal feces), fetal tissues, and placenta. c. jejuni colonies were identified in caruncles and placenta by light microscopy and immunoperoxidase techniques. histologically, inoculated ewes had a severe purulent endometritis with vasculitis. placentas fr ...19873672807
factors affecting the survival of campylobacter jejuni in relation to immersion scalding of poultry.potential measures for reducing the survival of campylobacters during commercial scalding of poultry have been evaluated in a series of laboratory trials. at 50 degrees c, the lower temperature limit of commercial scalding, raising the ph of a buffered heating medium from 6.0 to 9.0 markedly increased the heat sensitivity of campylobacter jejuni but the effect was largely nullified in the presence of 1 per cent 'organic material' (50:50 horse blood and milk). either in the presence or absence of ...19873672830
guillain-barré syndrome following campylobacter jejuni enteritis. 19873675255
survival of thermophilic campylobacters on fingertips and their elimination by washing and disinfection.a simple impression-plate technique has been used to investigate the survival of four thermophilic campylobacter strains applied to fingertips. campylobacters suspended in 0.1% peptone water and dried on the fingertips survived for different periods of time ranging from less than 1 to greater than or equal to 4 min. however, campylobacters suspended in chicken liquor or blood survived for much longer periods. the most resilient organism was campylobacter jejuni nctc 11392 which, when suspended i ...19873678391
serogroups of thermophilic campylobacters from humans and from non-human sources, israel 1982-1985.the distribution of serogroups of thermophilic campylobacters isolated in israel from human patients (2421 isolates), chicken (942), turkeys (158), cattle (398), wild birds (234) and other sources, was studied. among the human isolates, 74 rog-serogroups were identified. the six most commonly isolated of these (1, 18; 11; 12; 8,23; 4 and 5,39) were found frequently in chickens. only four common serogroups in man were also common in cattle, three in turkeys and two in wild birds. two common serog ...19873678392
[optimal conditions and affecting factors on adhesion of campylobacter jejuni to hela cells]. 19873679170
serotypes of campylobacter jejuni isolated from patients attending a diarrhoeal disease hospital in urban bangladesh.the serotypes of strains of campylobacter jejuni isolated from patients attending the international center for diarrhoeal disease research, bangladesh (icddr, b), dhaka, and from animals were ascertained by penner and hennessy's (1980) serotyping scheme. of 102 isolates from man, 74% were typable and serotypes 53, 15 and 22 predominated. of 26 isolates from animals, 65% were typable and serotypes 15 and 53 occurred frequently. the diarrhoeal illnesses associated with different serotypes were sim ...19873694663
approaches to the detection of enteric pathogens, including campylobacter, using nucleic acid hybridization.a previous study of the detection of campylobacter jejuni in fecal samples spotted directly onto nitrocellulose filters before hybridization revealed a relatively low sensitivity and some false-positive results. we have investigated two factors that interfere with the detection of campylobacter jejuni in fecal samples: interfering substances that create false-positive background signals and nonspecificity of the probe. heterologous deoxyribonucleic acid probes bound nonspecifically to partially ...19863698541
detection of pathogenic campylobacter species in blood culture systems.because differences in recognition of campylobacter fetus and campylobacter jejuni in systemic infections may be due partially to differences in the ability to cultivate these organisms, we studied their growth characteristics in two widely used blood culture systems. in the roche septi-chek system (hoffman-la roche, inc., nutley, n.j.), over a broad range of inocula all strains were detected in broth within 2 days and on paddles within 3 days. in the bactec 6b aerobic bottles (johnston laborato ...19863700626
the protection of infant mice from colonization with campylobacter jejuni by vaccination of the dams.intraperitoneal vaccination of female mice, before mating, with a whole cell, heat-killed (62 degrees c) vaccine of campylobacter jejuni allowed the mother to confer immunity to her young, challenged orally 4-6 days after birth with the homologous strain. there was no protection against a strain of another serotype. heating the vaccine to 100 degrees c destroyed its protective properties. a vaccine prepared from an aflagellate variant of the original strain was as protective as the original vacc ...19863701036
campylobacter jejuni in broilers: the role of vertical transmission.the role of broiler eggs in the transmission of campylobacter jejuni to broiler grow-out flocks was investigated. six breeder flocks supplying broiler eggs to hatcheries were examined for cloacal carriage of c. jejuni. of 240 birds tested, 178 (74%) were c. jejuni-positive. eggs from these birds examined for c. jejuni penetration of the egg shell indicated that 185 of 187 were campylobacter-free. eggs from breeder flocks of unknown c. jejuni status were also examined for c. jejuni shell penetrat ...19863701037
the cellular fatty acid composition of campylobacter species isolated from cases of enteritis in man and animals.the cellular fatty acid composition of 41 strains of suspected campylobacter jejuni, 23 from human cases of gastroenteritis and 18 from animals, was examined by gas-liquid chromatography. three of the 23 human isolates and 2 of 18 animal isolates did not contain 19:0 cyclopropane fatty acid and were identified as c. laridis. the remaining 36 strains had cellular fatty acid profiles consistent with c. jejuni but could be divided into three groups on the ratio of the concentration of 18:1 and 19:0 ...19863701040
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni. 19863703054
ovine abortion associated with campylobacter jejuni. 19863705362
persistence of multiply antibiotic-resistant campylobacter jejuni in a patient with the acquired immune deficiency syndrome.chronic diarrhea due to campylobacter jejuni has been described in patients with hypogammaglobulinemia. a patient with the acquired immune deficiency syndrome (aids) and normal total serum immunoglobulins had persistent diarrhea and c. jejuni on stool culture for seven and a half months despite repeated antibiotic therapy. antibiotic sensitivity studies revealed the c. jejuni to be multiply antibiotic-resistant. evaluation of the mechanism of resistance showed the organism harbored a conjugative ...19863706381
susceptibility of campylobacter jejuni to strain-specific bactericidal activity in sera of infected patients.campylobacter jejuni is a common cause of inflammatory enteritis, which in normal hosts is usually self-limited and resolves without antibiotic therapy. c. jejuni bacteremia is very rare. we examined sera for bactericidal activity that might be important in limiting the extent of c. jejuni infection in man. we studied the ability of nonimmune sera and homologous and heterologous immune sera from infected patients to kill different fresh case isolates of c. jejuni in vitro. the reduction of the l ...19863710581
enzyme-linked immunosorbent assays for virulence properties of campylobacter jejuni clinical isolates.to evaluate the capacity of enzyme-linked immunosorbent assays (elisas) to identify pathogenic strains among clinical fecal isolates of campylobacter jejuni, 40 consecutively obtained strains from 39 sick patients and 1 asymptomatic person were tested by respective elisas for enterotoxin production in culture filtrates and for the invasive virulence antigen of bacterial cells. of the 40 strains, 14 produced the enterotoxin; 15 strains, two of which were also enterotoxigenic, were invasive; and 1 ...19863711295
Displaying items 801 - 900 of 12694