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mixed gastric infection by gastrospirillum hominis and helicobacter pylori. 19901975013
antibodies to helicobacter pylori in hiv infection. 19901975069
treatment of helicobacter pylori positive duodenal ulcers. 19901975930
gastroenterologists in sydney--histology and helicobacter. 19901976150
campylobacter. 19901976939
bird attack on milk bottles: possible mode of transmission of campylobacter jejuni to man.a case-control study was carried out to test the hypothesis that the rise in the rate of campylobacter jejuni infection in the brigend area of south wales during may was due to the consumption or handling of milk from bottles that had been attacked by birds. 32 of 36 cases meeting the case definition were interviewed, along with 2 controls per case, matched for age, sex, and area of residence. there were strong associations between campylobacter infection and doorstep delivery of milk bottles, a ...19901978882
evaluation of urease test, gram stain, culture, and histology in the detection of campylobacter pylori.to evaluate the commonly available tests for detection of campylobacter pylori infection in the human stomach, we prospectively performed endoscopy and biopsy from the antrum of the stomach in 121 consecutive patients. four tests, including the urease test, gram stain, culture, and the hematoxylin and eosin (h & e) stain were used to detect the presence of c. pylori. a c. pylori positive was defined by a positive culture or positive results in two of the other three tests. the sensitivity of the ...19901981231
epidemiology and treatment of gastric campylobacter pylori infection: more questions than answers.two-hundred and ten consecutive patients undergoing routine gastroscopy were additionally investigated for evidence of campylobacter pylori (c.p.). 106 patients were positive in one or more tests: 99.1% using a rapid urease detecting test (clo-test), 80.2% histology, 78.3% cytology and 60% culture. we found no difference between the clo-test results from biopsies taken from different parts of the stomach in individual patients. c.p. was found in 100% of patients with significant chronic antral g ...19901982107
restriction fragment length polymorphism for the identification of campylobacter jejuni-isolates.companion animals ("pets") are occasionally carriers of organisms pathogenic for man. in the present, study fecal samples of clinically inapparent animals with direct contact to 204 patients, suffering from campylobacter enteritis, were investigated for c. jejuni or c. coli (cjc). cjc positive animals were seen in the environment of only five patients (= 2.4%). by comparison of biotypes and serotypes of thermostable and thermolabile antigens from human and animal isolates no clear epidemiologica ...19901982490
diagnosis of campylobacter pylori gastritis.campylobacter pylori is a bacterium that inhabits gastric mucosa. it causes chronic active gastritis and is highly associated with duodenal ulcer. campylobacter pylori has a urease enzyme (not present in man), which allows diagnosis by a [14c]urea breath test. we compared two noninvasive tests, the breath test and serum elisa, to biopsy and histologic diagnosis. twenty-two patients who underwent gastroduodenoscopy for evaluation of possible peptic ulcer disease entered the study. the breath test ...19911984994
helicobacter pylori and zollinger-ellison syndrome.helicobacter pylori (previously campylobacter pylori) is almost invariably associated with chronic duodenal ulcer disease. the relationship between h. pylori infection and duodenal ulcer in zollinger-ellison syndrome is unknown. we investigated the frequency of h. pylori infection in zollinger-ellison syndrome and also what effect h. pylori infection had on gastric function in patients with zollinger-ellison syndrome. h. pylori infection was diagnosed based on a specific serologic (elisa) assay ...19911984998
isolation and biochemical and molecular analyses of a species-specific protein antigen from the gastric pathogen helicobacter pylori.a protein of mr 26,000 which was present in large quantities in extracts of cells of helicobacter pylori was purified to homogeneity by ammonium sulfate precipitation followed by gel filtration and reversed-phase chromatography or anion-exchange chromatography. the protein appeared to be associated with the soluble fraction of the cell, and antibodies raised against the protein were reactive with whole-cell lysates of a variety of h. pylori strains in a simple immunodot blot assay. this reaction ...19911987145
chemical characterization of campylobacter jejuni lipopolysaccharides containing n-acetylneuraminic acid and 2,3-diamino-2,3-dideoxy-d-glucose.lipopolysaccharides (lps) of four nonencapsulated strains of the human enteric pathogen campylobacter jejuni were chemically characterized. when applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (h. brade and c. galanos, eur. j. biochem. 122:233-237, 1982) gave better yields of lps than did extraction by the conventional hot phenol-water technique. constituents common to all lps were d-glucose, d-galactose, l-glycero-d-manno-heptose, 3-deoxy-d-manno ...19911987154
campylobacter diarrhoea and an association of recent disease with asymptomatic shedding in egyptian children.a hospital-based case-control diarrhoea survey was conducted in cairo, egypt to determine the age-specific frequency of campylobacter infection among diarrhoeic and non-diarrhoeic children aged new born to 5 years. campylobacter was the most common bacterial enteropathogen isolated from diarrhoeic stools. the overall prevalence of campylobacter isolations was 25.9% from stools of 143 diarrhoeic children compared to 15.2% of 132 non-diarrhoeic control children (p = 0.028) during the 4-month perio ...19911993455
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
evaluation of techniques for isolation, subcultivation, and preservation of helicobacter pylori.with sheep blood agar (sba), belo horizonte medium, and brussels campylobacter charcoal agar, 104 strains of helicobacter pylori were detected in 309 gastric biopsies. each medium revealed only 69 to 71% of the strains. ten strains grew solely on sba, and four strains each grew on belo horizonte medium and brussels campylobacter charcoal agar. subculturing of 50 fresh h. pylori isolates on sba revealed a progressive reduction in growth with increasing passage. thirty strains stopped growing betw ...19911993767
biochemical and serological characterization of campylobacter cryaerophila.sixty-two isolates of campylobacter cryaerophila were recovered from aborted porcine and bovine fetuses, from porcine, bovine, and equine feces, and from different tissues of a dead piglet. phenotypic characterization was carried out on all isolates, and the results were compared with those obtained with the reference strains of c. cryaerophila, c. jejuni, c. coli, c. laridis, and c. hyointestinalis. the ability of c. cryaerophila strains to grow under aerobic conditions at 16 degrees c was foun ...19911993768
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (macaca nemestrina)a new microaerophilic, spirally curved, rod-shaped bacterium was isolated from the gastric mucosa of a pigtailed macaque (macaca nemestrina). the gram-negative cells of this bacterium are oxidase, catalase, and urease positive and strongly resemble helicobacter pylori (campylobacter pylori) cells. like h. pylori, this organism does not metabolize glucose, does not reduce nitrate or produce indole, does not produce h2s from triple sugar iron agar, does not hydrolyze hippurate or esculin, and does ...19911995031
measles-associated diarrhea in hospitalized children in lima, peru: pathogenic agents and impact on growth.because the causes of measles-associated diarrhea are not well known, 0- to 5-year-old children presenting to the hospital with measles-associated diarrhea (cases, n = 77) or acute diarrhea only (controls, n = 77) were compared. growth and diarrheal morbidity were evaluated for 1 month after acute illness. campylobacter jejuni was more frequently isolated from cases (31%) than controls (16%; p = .03). rotavirus was absent in all cases versus 28% of controls (p less than .001). incidence density ...19911995722
yersinia enterocolitica o:3: an emerging cause of pediatric gastroenteritis in the united states. the yersinia enterocolitica collaborative study group.after an outbreak of yersinia enterocolitica infections among black children in atlanta, a seven-hospital study was conducted to determine the importance of this pathogen in other communities with large black populations. of 4841 stool specimens from patients with gastroenteritis examined between november 1989 and january 1990, y. enterocolitica, shigella, campylobacter, and salmonella were identified in 38, 49, 60, and 98 specimens, respectively; 34 (92%) of 37 y. enterocolitica isolates were s ...19911995741
campylobacter jejuni as a cause of acute diarrhoea in children: a study at an urban hospital in bangladesh.the importance of c. jejuni as an aetiological agent of childhood diarrhoea was investigated at an urban children's hospital in dhaka over a period of 1 year. c. jejuni was isolated from 25.5% of 102 diarrhoeal patients compared to 8.6% of 93 age and sex-matched healthy control children studied (p less than 0.002). the organism was isolated as a single pathogen in 17.6% of diarrhoeal patients. no c. coli was detected. the infection rate was significantly higher (p less than 0.05) amongst childre ...19911995935
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...19911999383
shuttle cloning and nucleotide sequences of helicobacter pylori genes responsible for urease activity.production of a potent urease has been described as a trait common to all helicobacter pylori so far isolated from humans with gastritis as well as peptic ulceration. the detection of urease activity from genes cloned from h. pylori was made possible by use of a shuttle cosmid vector, allowing replication and movement of cloned dna sequences in either escherichia coli or campylobacter jejuni. with this approach, we cloned a 44-kb portion of h. pylori chromosomal dna which did not lead to urease ...19912001995
[helicobacter pylori and chronic ulcer disease]. 19912002726
virulence of campylobacter jejuni for chicken embryos is associated with decreased bloodstream clearance and resistance to phagocytosis.the 11-day-old chicken embryo has been shown to be a useful animal model for comparing the virulence of human isolates of campylobacter jejuni. virulence in this system is associated with the ability to invade the chorioallantoic membrane and to survive and proliferate in vivo. in this study, the survival and multiplication of c. jejuni in the embryonic host was investigated. it was possible to enhance the virulence of a relatively avirulent c. jejuni strain by passaging it intravenously through ...19912004823
foodborne infections and poultry. 19912005608
studies of helicobacter pylori in a gastric mucosa in vitro animal model.a gastric mucosa in vitro model for studies of experimental helicobacter pylori infections has been developed. biopsy specimens were taken from pig gastric mucosa, infected with h. pylori, and cultured for up to 72 h. to determine the degree of h. pylori adhesion, specimens were vigorously rinsed by vortexing five times before measuring viable count and urease activity. the results showed that it is possible to culture pig gastric mucosa in vitro with maintained viability for at least 72 h. acco ...19912006397
pathogenetic role of helicobacter pylori in duodenal ulcer disease. multivariate analysis of factors affecting relapse.the pathogenesis of duodenal ulcer disease is multifactorial and the contribution of helicobacter pylori in relation to the other factors to the release of duodenal ulcer is unknown. to investigate this, we studied 147 patients with endoscopically proven healed ulcers. these patients were randomized to receive either placebo, misoprostol 200 micrograms or misoprostol 300 micrograms four times daily, and clinical, personal, physiological and endoscopic characteristics were obtained prospectively. ...19912007359
usefulness of a new serological test (bio-rad) to diagnose helicobacter pylori-associated gastritis.a semiquantitative serological test (g.a.p. test; bio-rad) to diagnose helicobacter (campylobacter) pylori-associated gastritis has recently become commercially available. this test was evaluated with sera from 72 dyspeptic patients with known h. pylori status. when the instructions provided by the manufacturer were applied strictly, the sensitivity of the test was only 32.7%. with a slight modification of the instructions, the performance characteristics were still unsatisfactory (specificity, ...19912007636
evaluation of an immunofluorescence assay for specific detection of immunoglobulin g antibodies directed against helicobacter pylori, and antigenic cross-reactivity between h. pylori and campylobacter jejuni.an immunofluorescence assay (ifa) for the detection of immunoglobulin g antibodies directed against helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms. the resulting anti-h. pylori titers were classified as follows: negative, less than or equal to 64; borderline, 128; and positive, greater than or equal to 256. by using these cri ...19912007640
campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness.seventy-eight aerotolerant campylobacter isolates were characterized phenotypically and by dna hybridization (hydroxyapatite method at 50 and 65 degrees c). two dna relatedness groups were found. (i) sixty-four strains belonged to aerotolerant campylobacter dna hybridization group 2. these organisms were isolated from humans, primarily with diarrheal illness, and animals on several continents. strains were aerotolerant at 30 and 36 degrees c and catalase negative or weakly catalase positive, gre ...19912007646
persistent erythema nodosum and asymptomatic campylobacter infection. 19912007678
therapy for acute infectious diarrhea in children.this article reviews current recommendations of therapy with antidiarrheal compounds and antimicrobial agents for acute infectious diarrhea in children. in most infants and children with acute infectious diarrhea, treatment with antidiarrheal compounds is not indicated. many of these compounds interfere with identification of enteropathogens in stool specimens, and the antimotility class has an overdose potential. antimicrobial therapy is given to reduce symptoms and to prevent the spread of inf ...19912007952
etiology and mechanisms of acute infectious diarrhea in infants in the united states.infectious diarrhea, caused by a wide variety of viral, bacterial and parasitic pathogens, is a common reason for morbidity and hospitalization for children in the united states. overall, rotavirus is the most common cause of acute diarrheal disease in infants. salmonella, shigella, and campylobacter are the most frequently isolated bacterial pathogens, and giardia and cryptosporidium are the parasites that most commonly produce acute infectious diarrhea. the mechanisms by which these enteropath ...19912007955
an outbreak of campylobacter jejuni gastroenteritis linked to meltwater contamination of a municipal well.a large outbreak of campylobacter jujuni gastroenteritis attributed to contamination of an unchlorinated municipal water system was investigated. unlike most previous summer outbreaks, this one began in early spring and was attributed to meltwater entering one or more municipal wells. 241 suspected cases were documented, but retrospective information from local health care workers suggested a much larger outbreak. 45 laboratory-confirmed cases participated in a case-control study which showed a ...19912009481
[ascitic fluid infection caused by campylobacter: 2 cases]. 19912010081
aggressive kaposi's sarcoma and campylobacter bacteremia in a female with transfusion associated aids.the vast majority of patients with kaposi's sarcoma (ks) of the epidemic, endemic or sporadic variety are men. although 35 percent of men with aids will develop ks, only three percent of women will develop this malignancy. to date, a single case report of transfusion associated ks in an hiv-infected female has been described in the medical literature. we report a second case. a 54-year-old hiv-infected female without other identifiable risk factors for aids developed disseminated ks six years af ...19912012111
[yersinia pseudotuberculosis in new world monkeys].it is reported on four cases of pseudotuberculosis in 2 saimiri sciureus, 1 callithrix jacchus/penicillata hybrid and 1 s. oedipus. the animals came from two different groups, one being infected with y. pseudotuberculosis serotype i, the other with y. pseudotuberculosis serotype ii. all cases showed intestinal infection of thermophile campylobacter. remarkable were severe haemorrhagic components and the distinct rhs-proliferation, especially in the mesenteric lymphatic nodes.19912015031
[late post-pneumonectomy hematogenous pyothorax. apropos of 3 cases].the authors describe three cases of patients who had previously undergone a pneumonectomy (two for cancer, one for bronchiectasis) with a favourable postoperative course, who subsequently developed pyothorax in the thoracic cavity. the bacteria isolated where in the first patient, pasteurella multocida; in the second, campylobacter foetus foetus; in the third, a variety of anaerobic organisms. the characteristic of the organisms allow us to exclude the possibility that these cases of pyothorax w ...19912018342
the helicobacter (campylobacter) pylori-colonized duodenal mucosa and gastric metaplasia.biopsies were obtained from non-ulcerated sites of the duodenum from 100 dyspeptic patients. helicobacter (campylobacter) pylori was cultivated from 19 of these biopsies. active chronic duodenitis (acd) was found in 17 biopsies and more than 5% gastric metaplasia in 20 biopsies. h. pylori as well as acd occurred with a significantly increased frequency when more than 5% gastric metaplasia was found in the duodenal biopsies. h. pylori on metaplastic tissue without acd was, however, seen in two ca ...19912018637
campylobacter: pathogenicity and significance in foods.in the last 10 years campylobacter jejuni has emerged as the most frequent cause of bacterial gastroenteritis in man. acute enterocolitis, the most common presentation of c. jejuni infection, can affect persons of all ages. c. jejuni has been found in virtually every country where investigations have been carried out. the frequent finding of dysenteric stools suggests that mucosal damage due to an invasive process analogous to that seen in shigellosis is important in the pathogenesis. campylobac ...19912018702
the use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases.the application of nucleic acid analyses to investigations of infectious disease outbreaks has resulted in useful molecular strain markers that distinguish the epidemic clone of a particular pathogen and help identify specific vehicles of infection. we have successfully used plasmid profile analysis, restriction endonuclease digestion of plasmid and whole-cell dnas, and nucleic acid hybridization to investigate recent outbreaks of foodborne diarrheal illness. plasmid analysis has been important ...19912018708
epidemiology of campylobacter enteritis.campylobacter enteritis is the commonest form of infective diarrhoea in most developed countries of the world. in england and wales laboratory reports indicate an annual incidence of about 85/100,000, but the true rate is probably nearer 1100/100,000. measured costs run to many millions of pounds per year. most infections are sporadic and believed to be foodborne; large outbreaks are infrequent and mostly due to the consumption of raw milk or unchlorinated water. raw meats and animal products, n ...19912018709
epidemiology of helicobacter pylori in an asymptomatic population in the united states. effect of age, race, and socioeconomic status.a causative role is now accepted for helicobacter (formerly campylobacter) pylori in type b gastritis, and evidence is accumulating that h. pylori infection plays a major contributory role in peptic ulcer disease. preliminary studies have reported that the prevalence of h. pylori infection increases with age, but detailed information on the prevalence of the bacteria in any defined population and on the factors that may influence the pattern of distribution remains scanty. in the present study, ...19912019355
isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea.campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ...19912019754
pulsed-field gel electrophoresis of campylobacter jejuni and campylobacter coli genomic dna and its epidemiologic application.genomic dna from 12 different campylobacter jejuni and 10 campylobacter coli isolates was digested with smai and analyzed by pulsed-field gel electrophoresis (pfge) and 16s rrna hybridization studies. although the two campylobacter species displayed species-specific restriction and hybridization patterns, significant intraspecies differences were observed. combined pfge and hybridization pattern analysis failed to provide any more epidemiologic information than was obtained from pfge restriction ...19912019755
comparison of selective medium supplemented with blood & a charcoal-based blood-free medium for primary isolation of campylobacters from human faeces.a recently developed charcoal-based, blood-free selective medium-charcoal cefoperazone deoxycholate agar (ccda) was compared with the conventional butzler's agar (ba) for primary isolation of enteric campylobacters from human stool specimens. ccda yielded higher percentage (93.6%) of positive isolations as compared to ba (76.6%). the rate of isolation on ccda was significantly higher (p less than 0.0001) than on ba. cost-wise also, the ccda medium was two times cheaper than ba. in addition to ca ...19912022396
better preservation of campylobacter jejuni/c. coli in a defined medium.campylobacter jejuni and c. coli strains were preserved at -10 degrees c in different stock media to determine their efficacy to preserve the organism for a longer period of time. an improved defined stock culture medium was developed for the organism by removal and effective neutralisation of the toxic metabolites. comparative study revealed that phosphate buffer saline (pbs), ph 6.7 supplemented with 0.2 per cent charcoal, 0.025 per cent fbp (ferrous sulphate, sodium metabisulphate and sodium ...19912022397
[recurrence of peptic ulcers and risk factors]. 19912022904
[gastric mucosa and peptic ulcers]. 19912022905
[etiology and physiopathology of peptic ulcers]. 19912022910
incidence of campylobacteriosis among patients with aids in los angeles county.the incidence and characteristics of campylobacteriosis among patients with acquired immune deficiency syndrome (aids) in los angeles county were assessed by matching the campylobacter and aids surveillance reporting registries for the years 1983-1987. campylobacter infection was reported in 29 (0.7%) of 4,433 aids cases. the average annual incidence of campylobacter among aids cases (519/100,000) exceeded the crude population rate by 39-fold and exceeded the rate among males aged 15-55 years by ...19912023099
[dyspepsia and helicobacter (campylobacter) pylori. results of a randomized therapeutic trial using amoxicillin versus placebos].a population of non ulcer dyspepsia outpatients with a normal gastroscopy was assessed. clinical complaints, the type of the gastritis, its activity and bacterial density were evaluated. helicobacter pylori (hp) was sought by histology, urease test and culture, on 3 distinct locations. hp was found in 62 p. cent of the patients, always in association with gastritis. urease test and histology had the same sensitivity in the detection of hp. a randomized double blind study with amoxicillin vs plac ...19912029180
[campylobacter fetus ssp fetus septicemia associated with a colonic abscess].campylobacter is considered to be an opportunistic agent. the authors relate an unusual case with campylobacter fetus ssp fetus (cf) septicemia and colic abscess. human campylobacteriosis is presumed to be a food-born disease related to contaminated animal products such as milk or meat. in some cases cf may be transmitted by drinking water or by fecal soiling via the hands. conventional treatment uses macrolides with decrease the duration of diarrhea and reduce the fecal excretion of cf. macroli ...19912029182
early onset neonatal sepsis with campylobacter jejuni: a case report.perinatal infections with bacteria belonging to the genus campylobacter are being increasingly recognized. we present a case of early onset neonatal sepsis with campylobacter jejuni (previously c. fetus ss. jejuni or vibrio jejuni). the infant was born prematurely at 31 weeks of gestation and presented with respiratory distress and frequent apnoea from birth. the chest x-ray film demonstrated reticulogranular pattern consistent with hyaline membrane disease. the infant was successfully treated w ...19912029921
[reactive arthritis]. 19912031108
[the occurrence and survival of campylobacter in sewage sludge].campylobacter was found only in raw sewage sludge samples of the high-rate municipal sewage treatment plant studied. campylobacters were isolated from 28.6% of the sewage sludge samples of grit tank and primary settling tank. 40% of the settled activated sludge samples contained campylobacter. campylobacter was never found in digested sludge of a mean sludge age of more than 90 days. the survival times of campylobacter in sewage sludge samples ranged between some hours and 10 days. therefore a l ...19912031408
environmental aspects of campylobacter infections.epidemiological data indicate high incidence of campylobacteriosis. improperly prepared poultry-products, unpasteurized milk as well as non-chlorinated drinking water were shown to be the main vehicles of campylobacter transmission to man. there is a lack of knowledge concerning the role of various environments in transmission of campylobacter. the review summarizes the present knowledge about occurrence and survival of campylobacters in various environments (sewage, sludge, surface water, drink ...19912031409
correlation between molecular size of the surface array protein and morphology and antigenicity of the campylobacter fetus s layer.the correlation between the molecular size of the surface layer protein (s protein) and both structure and antigenicity of the campylobacter fetus surface layer (s layer) was investigated in several clinical strains and their spontaneous variants which produce s proteins of molecular weights (mw) different from those of the parents. only three molecular sizes of the s proteins were observed (98, 127, and 149 kda) in the parental and variant strains. immunologically, the 98-kda protein and the 14 ...19912037362
[yersinia and campylobacter food poisoning]. 19912038688
relationship between the clostridium perfringens catq gene product and chloramphenicol acetyltransferases from other bacteria.the nucleotide sequence of the clostridium perfringens chloramphenicol acetyltransferase (cat)-encoding resistance determinant, catq, was determined. an open reading frame encoding a protein of 219 amino acids with a molecular weight of 26,014 was identified. although catq was expressed constitutively, sequences similar in structure to those found upstream of inducible cat genes were observed. the catq gene was distinct from the c. perfringens catp determinant. the deduced catq monomer had consi ...19912039197
lansoprazole, a novel benzimidazole proton pump inhibitor, and its related compounds have selective activity against helicobacter pylori.the activities of various types of antiulcer agents against helicobacter pylori (formerly called campylobacter pylori) strains were determined by an agar dilution method. among the compounds tested, two benzimidazole proton pump inhibitors, lansoprazole (ag-1749) and omeprazole, were found to have significant activities against this organism. the activity of lansoprazole was comparable to that of bismuth citrate, with mics ranging from 3.13 to 12.5 micrograms/ml, and fourfold more potent than th ...19912039199
structural analysis of the lipid a component of campylobacter jejuni ccug 10936 (serotype o:2) lipopolysaccharide. description of a lipid a containing a hybrid backbone of 2-amino-2-deoxy-d-glucose and 2,3-diamino-2,3-dideoxy-d-glucose.the chemical structure of campylobacter jejuni ccug 10936 lipid a was elucidated. the hydrophilic backbone of the lipid a was shown to consist of three (1----6)-linked bisphosphorylated hexosamine disaccharides. neglecting the phosphorylation pattern, a d-glucosamine (2-amino-2-deoxy-d-glucose) disaccharide [beta-d-glucosaminyl-(1----6)-d-glucosamine], a hybrid disaccharide of 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine [2,3-diamino-2,3-dideoxy-beta-d-glucopyranosyl-(1----6)-d-glucosamin ...19912040305
[current problems of the treatment of duodenal ulcer in children].having a large clinical material at their disposal, the authors base the necessity of the search and administration of more effective methods of duodenal ulcer treatment. analyze the therapeutic effect produced by the enkephalin analogs (dalargin and its combinations with trichopol, de-nol, oxacillin) in connection with infection with campylobacter . comparison of the clinico-endoscopic data with the histological and cytological findings enabled arriving at a conclusion about the pathogenetic ef ...19912041691
reiter's syndrome and associated arthritides.several distinct arthritic syndromes now have been recognized in hiv-infected persons. these comprise seronegative spondarthritis, including classic reiter's syndrome and psoriatic arthritis associated with hla-b27, and undifferentiated arthritis usually confined to the lower limbs, unassociated with other lesions, and unrelated to any known genetic marker. in such cases great care should be taken to exclude infection. in addition, a syndrome of short-lived but sometimes severe arthralgias also ...19912041887
antibodies to arthritis-associated microbes in inflammatory joint diseases.igm, igg and iga class antibodies against yersinia, salmonella, campylobacter and borrelia were determined by enzyme-linked immunosorbent assay (elisa) in a group of 340 unselected patients with a recent inflammatory joint disease. the control group consisted of 340 and 100 healthy blood donors using borrelia-elisa and other elisas, respectively. of all the patients, 27.4% had increased antibody levels against at least one of the microbes tested. the prevalence of positive antibody levels was hi ...19912041980
[epidemiologic outbreak of campylobacter infection]. 19912042198
bacterial antibodies in ankylosing spondylitis.antibodies to salmonellae, yersiniae, campylobacter jejuni, borrelia burgdorferi, klebsiella pneumoniae, escherichia coli, proteus mirabilis and chlamydia trachomatis were measured by elisa in the sera of 99 patients with ankylosing spondylitis. increased prevalence of iga and igg class antibodies against k. pneumoniae and of iga class against e. coli was observed in ankylosing spondylitis. no clear correlation between the disease activity and occurrence of antibodies was revealed. the results a ...19912044228
positive serum antibody and negative tissue staining for helicobacter pylori in subjects with atrophic body gastritis.helicobacter pylori is rarely found in gastric biopsy specimens from individuals with atrophic gastritis of the body mucosa. to determine if subjects with atrophic body gastritis have evidence of previous infection with h. pylori, immunoglobulin g antibody to h. pylori was measured by enzyme-linked immunosorbent assay in sera of 399 finnish subjects. in 124 subjects, multiple biopsy specimens from body and antrum had been evaluated for the presence of h. pylori by giemsa staining. antibody corre ...19912044906
[campylobacter pyloridis in gastric inflammatory and ulcerative pathology. an endoscopic and histopathological study in 70 patients]. 19912046967
[clinical features of campylobacteriosis in children].campylobacters were found in 158 (6.7%) of 2,346 patients who were followed up for some years. the disease was more common among patients aged 1 to 3 years, particularly during summer. the clinical features of the disease are under discussion.19912047173
[gastroduodenal pathology in pyelonephritis in children].the authors describe lesions of the upper alimentary tract in 91 children aged 4 to 15 years suffering from pyelonephritis. intragastric ph-metry, endoscopy and histological examination of biopsy specimens from the gastric and duodenal mucosa were carried out, as a result of which gastroduodenal pathology was diagnosed in 3/4 of the patients. in 69.4% of the children, biopsy specimens of the mucous membrane of the antrum of the stomach showed campylobacter pylori. such combined pathology require ...19912047174
epidemiologic aspects of shigellosis and other causes of dysentery in thailand.nearly 20% of children seen in the outpatient department of children's hospital in bangkok, thailand, for diarrheal disease had bloody diarrhea. shigella species and enteroinvasive escherichia coli--isolated from 13% and 2% of children with diarrhea, respectively--were the most frequent causes of bloody diarrhea. campylobacter species and nontyphoidal salmonella species were also isolated frequently but were much less often associated with bloody diarrhea. shigella species were rarely isolated f ...19912047642
[role of campylobacter pylori and mucous microflora in the pathogenesis of long-non-healing stomach ulcers].altogether 89 patients with long non-healing gastric ulcers were examined for campylobacter pylori (cp) and mucous microflora. it is shown that in patients with long non-healing gastric ulcers, cp was only demonstrable in 19.8% of cases whereas pathological microflora (candida and microbial associations) in 72.3% of cases. in patients with cp, the administration of de-nol, metronidazole and ampicillin were not sufficiently effective.19912048015
waterborne campylobacteriosis in northern norway.a suspected waterborne outbreak of presumed campylobacteriosis involving approximately 680 of the 1000 inhabitants in a community is described. twenty-two strains of campylobacter jejuni were isolated from patients. ten randomly chosen isolates were identified as biotype 1 lau 0:1 (n = 9) and biotype 2 pen 0:6, 7 (n = 1) and one from tap water as biotype 1 pen 0:19, 22. the majority of cases occurred within a period of 1 week. examination of human sera obtained during the first 2 weeks of the ep ...19912049283
effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products.pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ...19912049285
identification of thermotolerant campylobacter species from poultry using an enzyme-labelled oligonucleotide dna probe.a commercially available enzyme-labelled dna probe for human campylobacter strains has been tested and also found to hybridize with dna from c. jejuni and c. coli isolates from poultry. dna from 11 enteric, non-campylobacter organisms, included in the test as negative controls, failed to hybridize with the probe indicating that the probe might be used for identification of campylobacter from poultry too.19912049289
epidemiology of campylobacteriosis in a cohort of rural population near calcutta.over a 2-year period, 25 families comprising of 181 individuals of all ages were longitudinally observed for the excretion of campylobacter species. faecal samples were taken from all persons with diarrhoea. specimens were also taken from apparently healthy individuals and from domestic animals living within the confines of the study families at monthly intervals. the overall diarrhoea attack rate was 19 episodes per 100 person-years with peak incidence in the 1- to 4-year-old age group (76/100 ...19912050205
haemolytic anaemia after childhood escherichia coli o 157 .h7 infection: are females at increased risk?we conducted a 4-year retrospective cohort study to better define the risk of haemolytic anaemia and haemolytic uraemic syndrome (hus) in children following sporadic gastrointestinal infection with the o 157.h7 serotype of escherichia coli. of the 72 children infected with this organism, 9 (12.5%) developed haemolytic anaemia, 6 of whom had hus. no child in a cohort of 72 age-matched controls with campylobacter jejuni gastroenteritis developed haemolytic anaemia (p = 0.003). females had a signif ...19912050207
significance of flagella in colonization resistance of rabbits immunized with campylobacter spp.cross-protection among different lior and penner serogroups of campylobacter spp. was studied. rabbits were orally immunized by gastric feeding with campylobacter spp., and 27 to 30 days later, they were challenged with matched or unmatched serogroups by the removable intestinal tie adult rabbit diarrhea (ritard) procedure. when immunized animals were challenged with different lior serotypes, no protection against colonization was seen; however, when challenged with homologous lior serogroups, p ...19912050397
campylobacter bacteremia: clinical experience with three different blood culture systems at mayo clinic 1984-1990.the efficiency of three commercially available blood culture systems for isolating campylobacter sp. was investigated. thirteen of 189,688 blood cultures from patients seen at the mayo clinic from 1984 through 1990 were positive for campylobacter sp. eleven out of 13 blood cultures positive for campylobacter sp. were part of a complete three bottle set of cultures (isolator from du pont, roche septi-chek from hoffman-la roche, and non-vented tryptic soy broth from difco), none of them providing ...19912050426
study on the pathogenicity of campylobacter jejuni by modifying the medium.campylobacter jejuni has been documented as one of the major aetiological agents of diarrhoeal illness all over the world. studies revealed its pathogenicity by different assay methods, but none could be strongly recommended as a tool for differentiating toxigenic strains of c. jejuni. this study was an attempt to demonstrate better its pathogenicity by media modification. fifteen isolates of c. jejuni recovered from diarrhoeal patients at the international centre for diarrhoeal disease research ...19912051523
[endocarditis caused by campylobacter fetus]. 19912051801
[comparative evaluation of the effectiveness of the treatment of gastroduodenal campylobacter infection in children].schemes developed by the authors for antibacterial therapy of gastroduodenal bacterosis caused by campylobacter spp. in children are described. the data on the treatment of 56 patients with duodenal ulcer and gastroduodenitis are presented. the efficacy of the treatment with medicines under the control of the clinical, endoscopic and microbiological indices was compared. the study showed that de-nol, furazolidone and combination of trichopol (metronidazole) with vicair were the most efficient dr ...19912053825
quinolone resistance in campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine.eight hundred and eighty-three strains of campylobacter spp. isolated between 1982 and 1989 from human stools and poultry products were screened for quinolone resistance. in this period the prevalence of resistant strains isolated from poultry products increased from 0% to 14%. during the same period the prevalence in man increased from 0% to 11%. the emergence of quinolone resistance has implications for the identification of campylobacter up to species level: the susceptibility for nalidixic a ...19912055811
comparison of six media, including a semisolid agar, for the isolation of various campylobacter species from stool specimens.a recently described semisolid blood-free selective motility medium (ssm) (j. goossens, l. vlaes, i. galand, c. van den borre, and j. p. butzler, j. clin. microbiol. 27:1077-1080, 1989) was compared with two charcoal-based selective media (charcoal-based selective medium [csm] and modified charcoal cefoperazone deoxycholate agar [ccda]), two blood-based media (skirrow medium [skm] and campybap), and a passive, 0.65-microns-pore-size cellulose acetate membrane filter technique for the recovery of ...19912056033
cloned dna probes specific for the intracellular campylobacter-like organism of porcine proliferative enteritis.although the etiology of porcine proliferative enteritis is not understood, the consistent presence of intracellular campylobacter-like organisms (clos) in proliferating pig intestinal epithelial cells suggests that the organism is involved in the disease process. in order to obtain information about this organism, we generated and characterized specific dna probes to the intracellular clo which was purified without culturing. intracellular clos were isolated from mucosa by homogenization, filtr ...19912056034
effect of inoculum size on the phenotypic characterization of campylobacter species.the type strains of six campylobacter species or subspecies were examined in eight tests used for the identification of such organisms. false-positive results were obtained in certain tests (growth on 1% bile, brilliant green, selenite, trimethylamine-n-oxide, 2,3,5-triphenyl tetrazolium chloride, and minimal media) when an inoculum yielding 10(7) to 10(8) cfu/ml was used. each tolerance test was examined with blood, nutrient, and brucella agars as basal media. the type of basal medium used coul ...19912056060
effect of iron-dextran on lethality of nigerian isolates of campylobacter jejuni in mice.mice were orally and intragastically challenged with standard suspensions containing 1 x 10(9-10) colony forming units (c.f.us) per ml of strains "2" and "5" of campylobacter jejuni isolated in lagos. another group of mice received the same amount of c. jejuni and 1pc iron-dextran which was incorporated into the bacterial suspension ad mueller-hinton broth. two sets of control mice were included in the study. one set received only 0.2 ml of phosphate buffered saline while the second set received ...19912060004
complete sequence of the campylobacter jejuni glya gene encoding serine hydroxymethyltransferase.the complete nucleotide sequence of the campylobacter jejuni glya gene was determined and the amino acid (aa) sequence of its product, serine hydroxymethyltransferase (shmt), was deduced. the deduced polypeptide has 414 aa residues (mr 45,758). the aa sequences of c. jejuni and escherichia coli show 55.6% identity. comparative analysis of the aa sequences of the shmts of e. coli and c. jejuni identified two new putative functional domains. the translational product of the c. jejuni glya gene was ...19912060796
guillain-barré syndrome associated with campylobacter infection. 19912062607
a study of risk factors for campylobacter infection in late spring.a case-control study was carried out to investigate possible reasons for the large increase in the number of cases of infection caused by campylobacter species reported in the regional epidemiology section of manchester public health laboratory in late spring each year. the hypothesis tested was that a risk factor peculiar to campylobacter infection is responsible for this phenomenon. interviews about a wide variety of risk factors were conducted with 29 patients suffering from campylobacter ent ...19912062992
[bacterial diarrhea in infancy: epidemiologic study of 256 cases].infectious diarrhoea are common causes of morbility in children. although viral agents are responsible for the majority of cases of acute diarrhoea during infancy, bacterial infections are also well recognized as causes of them. 256 patients with diarrhoea due to a bacterial pathogen were studied to determine the importance of different epidemiologic factors. the study group comprised 151 boys (59%) and 105 girls (41%) aged between first month and 14 years. family history of acute diarrhoea was ...19912064148
chronic atrophic gastritis in a population at risk for gastric carcinoma.gastric mucosa of 585 patients at risk for gastric carcinoma and undergoing oesophago-gastroscopy for dyspeptic symptoms was examined histologically and histochemically. sixteen (2.7%) of the patients studied showed gastric carcinoma. the intestinal type of gastric carcinoma was the most common, present in males over 40 yrs old, and was found in 1:16. early cancers were present in 3 patients. chronic atrophic gastritis of the a, b and ab types was present in 32.8%. types a and ab were noted spor ...19912064339
[effect of fu-zheng qu-xie on gastric disease infected with campylobacter pyloridis].chinese herbal medicine and gentamycin were used separately in the treatment of 75 cases of gastric disease by campylobacter pyloridis (cp). surveying the changes of the gastrofiberscope, pathological test, bacteriology and immunology. this study, found that in the chinese herbal medicine group clinical symptoms were obviously improving the effective rate for cp being 80% (24/30), the death rate for cp 30% (9/30), and the effective rate for patho-histological changes 50% (15/30). differences of ...19912065393
[the occurrence of campylobacter spp. and salmonella spp. in gulls in northern germany].out of a total of 207 gulls--20 black headed gulls, 185 herring gulls and 2 common gulls--128 (62%) and 23 (11%) birds, respectively, were infected with campylobacter spp. (c.) and salmonella spp. c. jejuni was predominant in gulls less than two years old (89%) and c. coli in older birds (75%). furthermore, the infection rate with campylobacters was depending on the habitats of the birds. the rate for c. jejuni and c. coli, respectively, was in gulls from regional garbage dumps 78% and 4%, from ...19912065612
inactivation of campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaa but not flab is required for invasion.the role of the campylobacter jejuni flagella in adhesion to, and penetration into, eukaryotic cells was investigated. we used homologous recombination to inactivate the two flagellin genes flaa and flab of c. jejuni, respectively. mutants in which flab but not flaa is inactivated remain motile. in contrast a defective flaa gene leads to immotile bacteria. invasion studies showed that mutants without motile flagella have lost their potential to adhere to, and penetrate into, human intestinal cel ...19912065653
helicobacter pylori infection induces antibodies cross-reacting with human gastric mucosa.the authors' previous observation that many of the monoclonal antibodies against helicobacter pylori cross-react with the cells of the human gastric mucosa prompted them to investigate the possibility that gastric self-antigens cross-reacting with h. pylori could be involved in the immune response against this organism. it was found that three antibodies against h. pylori, cb-4, cb-10, and cb-14, that cross-react with the human gastric mucosa also intensely cross-reacted with murine gastric epit ...19912065920
helicobacter pylori and abo blood groups.a serological study was carried out to assess the prevalence of antibodies to helicobacter pylori and compare it with the distribution of abo blood groups. serum samples from 402 healthy blood donors were tested with an igg enzyme linked immunosorbent assay. there was no difference in blood groups between those who were seropositive and those who were seronegative, which suggests that blood group o is not a risk factor for acquiring h pylori infection.19912066433
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