| comparative virulence of clinical brachyspira spp. isolates in inoculated pigs. | classical swine dysentery is associated with the presence of the strongly beta-hemolytic brachyspira hyodysenteriae. however, multiple brachyspira spp. can colonize the porcine colon. since 2008, several brachyspira spp. not identified as b. hyodysenteriae by genotypic and/or phenotypic methods have been isolated from the feces of pigs with clinical disease typical of swine dysentery. in the current study, 8 clinical isolates, including 5 strongly beta-hemolytic and 3 weakly beta-hemolytic brach ... | 2012 | 22956484 |
| phenotypic and molecular characterization of a novel strongly hemolytic brachyspira species, provisionally designated "brachyspira hampsonii". | since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the united states and canada. though the primary causative agent of swine dysentery is brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic brachyspira sp. this novel brachyspira sp. was identified from clinical submissions at the minnesota veterinary diagnostic laboratory, and 40 of such isolates were obtained from 22 clinical ... | 2012 | 22914820 |
| application of real-time pcr for detection of lawsonia intracellularis and brachyspira hyodysenteriae in fecal samples from pigs. | the aim of the study was to develop and validate real-time pcr method for the quantification of lawsonia intracellularis and brachyspira hyodysenteriae in porcine feces. before the optimization process was performed two different extraction methods were compared to select the more efficient one. based on the results achieved at this stage the boiling procedure was rejected and a commercially available silica-membrane based method was chosen for further analysis. the primers and the taqman probe ... | 2012 | 22844704 |
| antimicrobial susceptibility of brachyspira hyodysenteriae isolated from 21 polish farms. | swine dysentery (sd) is a common disease among pigs worldwide, which contributes to major production losses. antimicrobial susceptibility testing of b. hyodysenteriae, the etiological agent of sd, is mainly performed by the agar dilution method. this method has certain limitations due to difficulties in interpretation of results. the aim of this study was the analysis of antimicrobial susceptibility of brachyspira hyodysenteriae (b. hyodysenteriae) polish field isolates by broth microdilution pr ... | 2012 | 22844703 |
| comparison of atypical brachyspira spp. clinical isolates and classic strains in a mouse model of swine dysentery. | multiple brachyspira spp. can colonize the porcine colon, and the presence of the strongly beta-hemolytic brachyspira hyodysenteriae is typically associated with clinical swine dysentery. recently, several brachyspira spp. have been isolated from the feces of pigs with clinical disease suggestive of swine dysentery, yet these isolates were not identified as b. hyodysenteriae by genotypic or phenotypic methods. this study used a mouse model of swine dysentery to compare the pathogenic potential o ... | 2012 | 22770518 |
| elevated fat skatole levels in immunocastrated, surgically castrated and entire male pigs with acute dysentery. | boar taint is due to androstenone and skatole (3-methyl-indole) accumulation in fat tissues. during a study to investigate the effect of immunocastration on fattening pigs, an outbreak of acute dysentery occurred caused by lawsonia intracellularis and brachyspira hyodysenteriae and resulted in cachexia and high mortality. low androstenone levels in the immunocastrates (0.25 ± 0.04 μg/g liquid fat) suggested that the immunocastration had been effective, but unusually high skatole concentrations i ... | 2012 | 22613221 |
| development of a modified selective medium to enhance the recovery rate of brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces. | the aim of this study was to develop a modified selective medium to improve the recovery rate of brachyspira hyodysenteriae and other clinically significant intestinal spirochaetes from porcine faeces. | 2012 | 22283457 |
| phenotypic and molecular characterization of brachyspira spp. isolated from wild rodents. | the occurrence of intestinal spirochaetes of genus brachyspira in wild rodents was studied by cultivating 209 caecal samples. spirochaetal cultures were obtained from 83% of rats and 33% of house mice. biochemical characterization and six different species-specific pcr methods were applied to 101 of 118 isolates and a selection of 34 brachyspiras were further studied by sequencing of the 16s rrna gene. the results showed that isolates representing all the established biochemical phenotypes could ... | 2010 | 23766276 |
| toxigenicity of treponema hyodysenteriae, another emerging enteropathogen. | | 1982 | 27709524 |
| an optimized swine dysentery murine model to characterize shedding and clinical disease associated with "brachyspira hampsonii" infection. | the development of a mouse model as an in vivo pathogenicity screening tool for brachyspira spp. has advanced the study of these economically important pathogens in recent years. however, none of the murine models published to date have been used to characterize the clinical signs of disease in mice, instead focusing on pathology following oral inoculation with various brachyspira spp. the experiments described herein explore modifications of published models to characterize faecal consistency, ... | 2017 | 28830508 |
| a review of methods used for studying the molecular epidemiology of brachyspira hyodysenteriae. | brachyspira (b.) spp. are intestinal spirochaetes isolated from pigs, other mammals, birds and humans. in pigs, seven brachyspira spp. have been described, i.e. b. hyodysenteriae, b. pilosicoli, b. intermedia, b. murdochii, b. innocens, b. suanatina and b. hampsonii. brachyspira hyodysenteriae is especially relevant in pigs as it causes swine dysentery and hence considerable economic losses to the pig industry. furthermore, reduced susceptibility of b. hyodysenteriae to antimicrobials is of incr ... | 2017 | 28757022 |
| presence and mechanisms of acquired antimicrobial resistance in belgian brachyspira hyodysenteriae isolates belonging to different clonal complexes. | swine dysentery (sd) is an economically important disease for which antimicrobial treatment still occupies an important place to control outbreaks. however, acquired antimicrobial resistance is increasingly observed in brachyspira hyodysenteriae. in this study, the minimal inhibitory concentrations (mic) of six antimicrobial compounds for 30 recent belgian b. hyodysenteriae isolates were determined using a broth microdilution method. in addition, relevant regions of the 16s rrna, 23s rrna and th ... | 2017 | 28757011 |
| metabolic response of porcine colon explants to in vitro infection by brachyspira hyodysenteriae: a leap into disease pathophysiology. | swine dysentery caused by brachyspira hyodysenteriae is a production limiting disease in pig farming. currently antimicrobial therapy is the only treatment and control method available. | 2017 | 28706469 |
| in vitro susceptibility of brachyspira hyodysenteriae to a commercial citrus fruit extract. | brachyspira hyodysenteriae is the main etiological agent of swine dysentery (sd). nowadays, treatment and control of sd is increasingly difficult due to the emergence of antimicrobial resistance together with the restrictions on the use of antibiotics in veterinary practice. the aim of this study was to evaluate, as an alternative in the control of this disease, the antimicrobial activity and the main mechanism of action of biocitro, a citrus extract commercialized as raw material and used as fe ... | 2017 | 28651094 |
| neutrophil elastase and il17 expressed in the pig colon during brachyspira hyodysenteriae infection synergistically with the pathogen induce increased mucus transport speed and production via mapk3. | brachyspira hyodysenteriae colonizes the pig colon resulting in mucoid hemorragic diarrhea and mucus layer changes. these changes are characterized by a disorganized mucus structure and a massive mucus induction with de novo expression of muc5ac and increased production of muc2. to investigate the mechanisms behind this altered mucin environment, we quantified the mrna levels of mucin pathway genes and factors from the immune system in the colon of infected and control pigs, revealing upregulati ... | 2017 | 28559407 |
| brachyspira hyodysenteriae and b. pilosicoli proteins recognized by sera of challenged pigs. | the spirochetes brachyspira hyodysenteriae and b. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (sd) and porcine intestinal spirochaetosis (pis), respectively. although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against sd ... | 2017 | 28522991 |
| characterization of brachyspira hyodysenteriae isolates from italy by multilocus sequence typing and multiple locus variable number tandem repeat analysis. | to evaluate and compare the capabilities of multilocus sequence typing (mlst) and multiple locus variable number tandem repeat analysis (mlva) techniques to characterize brachyspira hyodysenteriae isolates and to investigate the relationship between pleuromutilin resistance and genetic variability. | 2017 | 28510989 |
| emergence of brachyspira species and strains: reinforcing the need for surveillance. | this short review discusses the increasing complexity that has developed around the understanding of brachyspira species that infect pigs, and their ability to cause disease. it describes the recognition of new weakly haemolytic brachyspira species, and the growing appreciation that brachyspira pilosicoli and some other weakly haemolytic species may be pathogenic in pigs. it discusses swine dysentery (sd) caused by the strongly haemolytic brachyspira hyodysenteriae, particularly the cyclical nat ... | 2015 | 28694985 |