| skeletal microcirculatory responses to hyperdynamic escherichia coli sepsis in unanesthetized rats. | to determine the microvascular site of vasodilation during hyperdynamic sepsis, we measured arteriolar and venular responses to live escherichia coli bacteremia in the rat cremaster muscle by direct in vivo videomicroscopy. our data indicate that cardiac output (by thermodilution) increased, systemic vascular resistance decreased, and a differential arteriolar response occurred, with constriction of large arterioles and dilation of small terminal arterioles. we conclude that dilation of small te ... | 1987 | 3541855 |
| structural and functional relationships between fumarase and aspartase. nucleotide sequences of the fumarase (fumc) and aspartase (aspa) genes of escherichia coli k12. | the nucleotide sequences of two segments of dna (2250 and 2921 base-pairs) containing the functionally related fumarase (fumc) and aspartase (aspa) genes of escherichia coli k12 were determined. the fumc structural gene comprises 1398 base-pairs (466 codons, excluding the initiation codon), and it encodes a polypeptide of mr 50353 that resembles the fumarases of bacillus subtilis 168 (citg-gene product), rat liver and pig heart. the fumc gene starts 140 base-pairs downstream of the structurally- ... | 1986 | 3541901 |
| effect of methylprednisolone, indomethacin, and diethylcarbamazine on survival rate following trauma and sepsis in rats. | the role of steroids in the treatment of sepsis and septic shock remains controversial, and it is not known if a possible beneficial effect is due to inhibition of the cyclooxygenase or lipoxygenase pathway of arachidonic acid metabolism. in this investigation we studied the effect of methylprednisolone (mp), the cyclooxygenase inhibitor indomethacin (im), and the lipoxygenase inhibitor diethylcarbamazine (de) on survival rate in an experimental trauma-sepsis model in rats consisting of laparoto ... | 1986 | 3542276 |
| conservation and antigenicity of n-terminal sequences of gp185 from different plasmodium falciparum isolates. | complementary dna (cdna) clones for gp185, a major antigenically diverse glycoprotein of plasmodium falciparum, were isolated from a cdna library of the honduras i/cdc (honduras i) isolate, and 1052 bp were sequenced. the expression of cdna fragments in escherichia coli using the vector pcqv2 allowed verification of the reading frame. this gp185 cdna sequence, like the cdna sequence for a homologous gene of the k1 isolate [hall et al., nature 311 (1984) 379-382], codes for a polypeptide which is ... | 1986 | 3542719 |
| the primary structure of rat ribosomal protein l19. a determination from the sequence of nucleotides in a cdna and from the sequence of amino acids in the protein. | the covalent structure of rat ribosomal protein l19 was inferred from the sequence of nucleotides in a recombinant cdna and confirmed from the sequence of amino acids in a portion of the protein. ribosomal protein l19 contains 196 amino acids and has a molecular weight of 26,971. there are indications that a segment of 23 residues in rat l19 is related to sequences of the same length in escherichia coli ribosomal proteins l30, l18, and s2. | 1987 | 3542997 |
| [direct and mediated action of a permanent magnetic field on biological objects]. | the effect of a constant magnetic field (cmf) of h = 2.3 x 10(5) a/m (2900 oe) on the viability and radiosensitivity of e. coli b and the effect of magnetically activated water (maw) on the radioresistance of rats were examined. the exposure did not influence the growth kinetics of e. coli b. cell cultivation in the magnetically pretreated nutrient medium enhanced the bacterial growth. preliminary exposure of bacterial cells to a cmf for 24 and 48 hrs increased and that for 72 hrs decreased thei ... | 1986 | 3543486 |
| rat pulmonary artery restructuring and pulmonary hypertension induced by continuous escherichia coli endotoxin infusion. | we have studied the effect of continuous endotoxin infusion on rat pulmonary structure and function (69.4 ng/100 gm body weight/min for 24 hours). after 6 days of endotoxin infusion, lack of filling of pre- and intraacinar arteries was evident on pulmonary arteriograms. microscopy demonstrated lumen narrowing in preacinar arteries and occlusion of intraacinar arteries. morphometry of patent intraacinar arteries established dilation and increased wall muscle. widespread alveolar wall injury was e ... | 1987 | 3543489 |
| protein and energy metabolism in liver tissue following intravenous infusion of live e. coli bacteria in rats. | in the present study protein synthesis and energy level in liver tissue were studied in bacteremic rats following intravenous infusion of 8 +/- 4 x 10(9) live e. coli bacteria and in control animals receiving a corresponding infusion of sterile saline. for the study of protein synthesis, liver slices were incubated in a medium containing 14c-leucine and incorporation rate of amino acid into protein was determined. hepatic concentrations of atp, adp, and amp were measured and energy charge (ec) w ... | 1987 | 3545530 |
| [phospholipase a from the pancreas and e. coli]. | | 1986 | 3547505 |
| metabolic conversion of tri- and tetrachloroethylene: formation and deactivation of genotoxic intermediates. | | 1986 | 3549228 |
| [effect of armin on nonspecific resistance factors of the body and on the primary humoral immune response]. | a dose-dependent increase of parameters of non-specific defence of the organism and depression of primary humoral immune response following a single subcutaneous administration of armin in doses of 0.04-0.16 mg/kg were found during experiments on mice and rats. | 1987 | 3549354 |
| susceptibility to experimental candida albicans urinary tract infection in the rat. | the urinary tract is resistant to ascending infection due to candida albicans. host and microbial factors that may alter such resistance were evaluated in the rat after inoculating c. albicans into the urinary tract. diuresis, diabetes, candidal germ-tube formation, and vaginal candida colonization (alone or in concert) failed to promote ascending urinary tract infection with two vaginal isolates of c. albicans capable of producing renal infection by the hematogenous route. both germinated and u ... | 1987 | 3549921 |
| in vivo evaluation of tigemonam, a novel oral monobactam. | tigemonam, a new monobactam with excellent activity against gram-negative bacteria, was evaluated for in vivo efficacy and absorption after oral administration to laboratory animals. tigemonam is absorbed when administered orally to mice and dogs. in a variety of gram-negative systemic infections in mice, orally administered tigemonam was efficacious in all infections studied. comparison drugs such as amoxicillin, cephalexin, and cefaclor were less efficacious, especially in infections caused by ... | 1987 | 3551830 |
| dexamethasone-prepared escherichia coli-induced disseminated intravascular coagulation. animal model. | we have developed a new model of disseminated intravascular coagulation in rats based on the induction of immunosuppression by prolonged high-dose dexamethasone treatment. most models of disseminated intravascular coagulation are based on the generalized shwartzman reaction, which is observed characteristically in experimental animals after two separate inoculations of bacterial endotoxins. these produce massive deposition of thrombi in the microcirculation and significant hemorrhagic and ischem ... | 1987 | 3553737 |
| the primary structure of rat ribosomal protein l7. the presence near the amino terminus of l7 of five tandem repeats of a sequence of 12 amino acids. | the covalent structure of rat ribosomal protein l7 was determined in part from the sequence of nucleotides in a recombinant cdna and in part from the sequence of amino acids in portions of the protein. the complementary analyses supplemented and confirmed each other. ribosomal protein l7 contains 258 amino acids and has a molecular weight of 30,040. the protein has an unusual and striking structural feature near the nh2 terminus: five tandem repeats of a sequence of 12 residues. rat l7 appears t ... | 1987 | 3624274 |
| effects of dietary lactose and purified diet on intestinal microflora of rats. | in the rats fed on lactose-containing purified diet (pdl), bifidobacteria remarkably increased in number in both the jejunum and cecum as compared with those fed on control diet (pd) or conventional diet (nmf). besides, lactobacilli, streptococci, staphylococci, and escherichia coli increased in number in the jejuna and/or ceca of rats fed on pdl as compared with those fed on pd. the incidence and numbers of lactobacillus species were remarkably different between the rats fed on pdl and those on ... | 1987 | 3626132 |
| transcriptional control of rat heme oxygenase by heat shock. | a heat shock element is located in the 5'-flanking region of the rat heme oxygenase gene (ho gene). the incubation of rat glioma cells at 42 degrees c or with hemin at 37 degrees c increased the levels of heme oxygenase mrna within 1 h and produced a maximum at 3 h (at least a 20-fold increase). in both treatments, the heme oxygenase activity started to increase after a lag period of about 1 h and reached a maximum value at 5 h. there was an apparent additive effect of both treatments on the hem ... | 1987 | 3654594 |
| role of anaerobic flora in the translocation of aerobic and facultatively anaerobic intestinal bacteria. | it is thought that the normal enteric microflora acts not only to prevent intestinal colonization but also to prevent subsequent systemic dissemination of ingested, potentially pathogenic bacteria. to determine the relative roles of specific components of the intestinal bacterial flora in bacterial translocation out of the gut, mice were given various antimicrobial agents to selectively eliminate specific groups of intestinal bacteria. the cecal flora and the translocating bacteria in mesenteric ... | 1987 | 3666959 |
| alteration of chemotherapy toxicity using a chemically defined liquid diet in rats. | controversy exists as to whether administration of a chemically defined diet alters toxicity to chemotherapy. the purpose of this study was to evaluate toxicity to methotrexate in rats fed a chemically defined liquid diet or a regular chow diet. in the first study, 48 adult rats were randomized to be fed a chemically defined liquid diet or a regular diet for 14 days when methotrexate (25 or 50 mg/kg) was given. all liquid diet rats became anorexic and died within 96 h, while no deaths were obser ... | 1986 | 3731073 |
| nucleotide sequence of cloned cdna specific for rat ribosomal protein l35a. | a cdna clone specific for rat ribosomal protein l35a, which is known to be a trna-binding protein, was isolated by hybrid-selected translation from a cdna library made for 8-9-s poly(a)-rich rna from regenerating rat liver. the nucleotide sequence of the cdna was determined. it consists of one base pair from the 5' leading sequence, the entire coding sequence of 333 base pairs and 14 base pairs from the 3' trailing sequence. the primary structure of protein l35a was deduced from the nucleotide s ... | 1986 | 3753935 |
| [interaction of glucocorticoid hormones with mitochondrial membranes]. | the mechanism of 3h glucocorticoid binding with the rat liver mitochondria in vitro is investigated. the linear dependence of the amount of bound hormones on the concentration of the free ones is shown and no saturation in the region of the physiological concentrations is observed. a very low specific binding in the presence of a 100-fold excess of an unlabelled hormone is found. the outer mitochondrial membranes binds a considerably higher amount of steroids, than the inner one. the binding of ... | 1987 | 3810892 |
| kupffer cell modulation of the systemic immune response. | the effects of global hepatic injury and of kupffer cell activation on systemic immunity were studied in an in vivo rat model, using the diameters of the delayed-type hypersensitivity (dth) response to keyhole limpet hemocyanin and of a subcutaneous staphylococcus aureus abscess as measures of systemic immunoresponsiveness. hepatic injury with carbon tetrachloride resulted in significant suppression of the dth score (5.5 +/- 0.7 vs 8.8 +/- 0.8 mm). kupffer cell activation with intraportal escher ... | 1987 | 3813868 |
| nucleotide sequence of cloned cdna specific for rat ribosomal protein s11. | a cdna clone specific for rat ribosomal protein s11 was isolated by hybrid-selected translation from the cdna library made for 8-9 s poly(a) rna from regenerating rat liver. since this cdna had not enough length, another clone was selected by colony hybridization using a fragment of isolated cdna as a probe. the nucleotide sequence of the cdna was determined. the sequence contains 2 base pairs from the 5' noncoding region, the entire coding region of 477 base pairs, and the 3' noncoding region o ... | 1985 | 3838984 |
| molecular cloning of the complementary dna for human tumor necrosis factor. | tumor necrosis factor (tnf) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. human tnf was purified to apparent homogeneity as a 17.3-kilodalton protein from hl-60 leukemia cells and showed cytotoxic and cytostatic activities against various human tumor cell lines. the amino acid sequence was determined for the amino terminal end of the purified protein, and oligodeoxyribonucleotide probes were synthesized on the basis of this sequence. complementary dna ... | 1985 | 3856324 |
| production of neutralizing monoclonal antibodies to escherichia coli heat-stable enterotoxin. | in an effort to develop new approaches to the study and control of infectious diarrhea, we prepared murine monoclonal antibodies to the escherichia coli heat-stable enterotoxin (sta). the toxin was purified from e. coli culture media and conjugated to bovine serum albumin. the sta-bovine serum albumin conjugate was used to immunize balb/c mice, and the immune spleen cells from these mice were fused with sp2/0 myeloma cells. resultant hybridomas were screened in an enzyme-linked immunosorbent ass ... | 1985 | 3880723 |
| host immune status in uraemia. vi. leucocytic response to bacterial infection in chronic renal failure. | infection often complicates renal failure and frequently causes death, but the association between renal failure, impaired immunity and infection has not been proved. a recent study showed that patients on dialysis did not show an expected leucocytic response to infection, suggesting that the blunted response was evidence of the immunocompromised state of the uraemic patient. in this study, the relationship between leucocytic responses and infectious challenge was investigated in an animal model ... | 1985 | 3881687 |
| alteration of adipocyte calcium homeostasis by escherichia coli endotoxin. | the present study evaluated calcium homeostasis in rat adipocytes after either in vivo or in vitro exposure to escherichia coli endotoxin. fat cells from endotoxin-treated rats showed an enhanced uptake of 45ca. in an attempt to differentiate between 45ca binding to the cell surface and intracellular 45ca accumulation, adipocytes were exposed to 5 mm lacl3. the amount of 45ca remaining associated with lanthanum-treated adipocytes was taken to be located intracellularly and was increased in adipo ... | 1985 | 3883814 |
| sublethal hemorrhage impairs the acute peritoneal inflammatory response in the rat. | hemorrhagic shock increases the risk of septic complications in injured patients. in this study, we investigated the effect of sublethal hemorrhage on the acute peritoneal inflammatory response and the clearance of bacteria from the peritoneal cavity of the rat. sprague-dawley rats were subjected to sublethal hemorrhage, resuscitated, and then inoculated intraperitoneally with a suspension of viable escherichia coli in saline. sham-hemorrhaged rats served as controls. sublethal hemorrhage decrea ... | 1985 | 3884827 |
| prostanoid production by lipopolysaccharide-stimulated kupffer cells. | although some data suggest that macrophages in the reticuloendothelial system (res) are important sources of thromboxane a2 (txa2) and prostacyclin (pgi2) during endotoxic shock, we are unaware of data documenting the ability of hepatic macrophages (kupffer cells) to release either txa2 or pgi2 when exposed to lipopolysaccharide (endotoxin, lps). in this study, kupffer cells were examined for their ability to release prostaglandin e2 (pge2), txa2, and pgi2 following stimulation with 0, 1.0, 50.0 ... | 1985 | 3887037 |
| biliary obstruction and susceptibility to biliary sepsis in rats. | the effect of retrograde intrabiliary (ri) injection of e. coli was studied in sprague-dawley rats with biliary obstruction of different duration (3 days; 2, 4, and 6 weeks). by the injection of 10(5) colony-forming units (cfu) immediately after occlusion of the common bile duct (cbd), 15 of 18 normal rats survived without clinical signs of infection. in contrast, six of 11 animals in 3-day obstruction (p = 0.04), seven of 12 in 2-week obstruction (p = 0.02), ten of 12 in both 4-week and 6-week ... | 1985 | 3887532 |
| [activation of the kallikrein-kinin system by endotoxin in the experimental shock model of the rat]. | | 1985 | 3887787 |
| [experimental study on acute lung injury induced by e. coli endotoxin in the rat]. | | 1985 | 3888554 |
| standardized intraabdominal abscess formation with generalized sepsis: pathophysiology in the rat. | gelatine capsules containing escherichia coli and bacteroides fragilis in a standardized mixture with rat colonic content and barium sulfate were implanted intraabdominally into rats. capsules of 0.75 g gave approximately 50% mortality whereas 0.35 and 1.10 g caused no or 100% mortality, respectively. in subsequent experiments, using the 0.75 g capsule, all animals became ill with signs of tachypnea, piloerection, low physical activity and hypersecretion of saliva 6-8 h after the implantation. t ... | 1985 | 3888637 |
| cross-reactive immunoprotective antibodies to escherichia coli o111 rough mutant j5. | the potential immunoprotective role of antiserum to an escherichia coli j5 mutant derived from e. coli o111:b4 was demonstrated in an experimental mouse model. overwhelming bacterial inocula masked the effects of cross-reactive immunoprotection due to antiserum to strain j5. enhanced bacterial clearance was observed in mice receiving antiserum to strain j5 in sublethal infections but not from lethal doses. incorporation of hemoglobin with the bacterial inocula decreased the 50% lethal dose of ch ... | 1985 | 3889175 |
| tissue distribution, developmental profiles and effect of denervation of enolase isozymes in rat muscles. | the tissue distribution of muscle-type alpha beta and beta beta enolases in rats were determined with the sandwich-type enzyme immunoassay method which utilized the purified antibodies specific to the alpha and to the beta subunit of enolase, and beta-d-galactosidase from escherichia coli as label. all the tissues examined contained detectable levels of both alpha beta and beta beta enolases. the beta beta enolase was found at high levels in the skeletal muscle tissues (tongue, esophagus, diaphr ... | 1985 | 3893549 |
| regulation of polyamine biosynthesis by antizyme and some recent developments relating the induction of polyamine biosynthesis to cell growth. review. | this review considers the role of antizyme, of amino acids and of protein synthesis in the regulation of polyamine biosynthesis. the ornithine decarboxylase of eukaryotic cells and of escherichia coli can be non-competitively inhibited by proteins, termed antizymes, which are induced by di- and poly- amines. some antizymes have been purified to homogeneity and have been shown to be structurally unique to the cell of origin. yet, the e. coli antizyme and the rat liver antizyme cross react and inh ... | 1985 | 3893559 |
| [effect of litonit and teturam on the course of biochemical processes in infectious inflammatory lesions of the kidneys in alcoholized animals]. | the time-course of changes in the activity of sorbitol dehydrogenase, catalase and the intensity of lipid peroxidation in the blood and kidneys of 250 albino rats was studied in the course of the development of alcoholic dependence when suffering from secondary infection. litonit, a new antialcoholic drug, was found to be effective for the treatment of infectious inflammatory renal lesions in alcoholism. unlike teturam, litonit promoted the decrease of sorbitol dehydrogenase and catalase activit ... | 1985 | 3893564 |
| the atp-dependent breakdown of proteins in mammalian mitochondria. | | 1985 | 3894107 |
| impaired liver clearance of bacteria in rats with chronic biliary obstruction. | 125i-labeled e. coli was injected into the biliary tree of normal rats and rats with 3 weeks' obstruction of the common bile duct to investigate the liver clearance capacity for bacteria. bile was collected during 15 min, immediately, 1 h, 4 h, or 24 h after the injection. tissue specimens from the liver, lungs, spleen and kidneys, and blood and urine specimens were collected simultaneously. in normal rats, 40% of the bacteria was recovered in the bile immediately after the injection, whereas 30 ... | 1985 | 3895335 |
| myocyte and nonmyocyte rna polymerase activity and chromatin template function. | myocardial and nonmyocardial cell nuclei were isolated from ventricles of adult male wistar rats by means of sucrose density centrifugation. rna polymerase activity in myocyte nuclei was approximately 80-90% higher than in nonmyocytes. total myocyte chromatin template activity using escherichia coli rna polymerase was linear and about onefold greater than the nonmyocyte fraction over a fivefold range of chromatin concentrations. preincubation time required for rna polymerase to form a stable bin ... | 1985 | 3895982 |
| [prolonged action preparation of cefaclor]. | this study was conducted to develop a prolonged action preparation of cefaclor (ccl) which can offer, with the twice-a-day administration, as much effectiveness as its conventional preparation (kefral capsule) with the 3 times-a-day administration. absorption site of ccl in gastrointestinal tract, preparation form (enteric coated granules) which slowly release ccl, dissolution property of the form, and mixed ratio of the form and rapid release form (nonenteric coated granules) were studied and c ... | 1985 | 3897611 |
| effect of the presence of black pigmented bacteroides of differing pathogenicity on the phagocytosis of escherichia coli by rat polymorphonuclear leucocytes. | an investigation was made of the ability of rat polymorphonuclear leucocytes (pmnls) to phagocytose escherichia coli in the presence of two species of black pigmented bacteroides (b. melaninogenicus and b. intermedius). when both the bacteria were opsonized together in the presence of normal rat serum, b. melaninogenicus and b. intermedius impaired the phagocytosis of e. coli significantly. however, the phagocytosis of these black pigmented bacteroides remained unaffected in the presence of e. c ... | 1985 | 3897814 |
| efficacy of bmy-28142 in experimental bacteremia and meningitis caused by escherichia coli and group b streptococci. | we evaluated the activity of bmy-28142 against a k1 e. coli strain and a type iii group b streptococcal strain in vitro and in vivo and compared the results with those of cefotaxime and penicillin g, respectively. in vitro, the mics and mbcs of bmy-28142 were close to those of cefotaxime (less than or equal to 2-fold difference) for e. coli and fourfold less than those of penicillin g for group b streptococci. in vivo studies with an experimental bacteremia and meningitis model in newborn rats r ... | 1985 | 3899006 |
| effects of bile, infection and pressure on pancreatic duct integrity. | ionic flux, potential difference and mucosal ultrastructure have been studied in the rat bile-pancreatic duct and the effects of pressure, bile and infection on the duct evaluated. the duct remained stable after perfusion with control solution under low pressure and high pressure produced widening of intercellular spaces only. perfusion with a bacterial solution of escherichia coli did not effect significant changes. sterile human bile disturbed the integrity of the duct by increasing ionic flux ... | 1985 | 3899241 |
| differential expression of the multiple forms of rat prekininogen mrnas after acute inflammation. | responses of the rat liver prekininogen mrnas after induction of acute inflammation were examined by blot-hybridization and s1 nuclease protection analyses with the aid of cdna probes specific for rat kininogens. marked changes in the relative levels of the low molecular weight (lmw) prekininogen mrnas were observed after administration of escherichia coli lipopolysaccharide, and the mrna levels increased with a half-maximal dose of approximately 100 ng of lipopolysaccharide/100 g body weight. a ... | 1985 | 3900068 |
| quantitation of s-adenosylmethionine decarboxylase protein. | a method for the specific labeling of the active site of s-adenosylmethionine decarboxylase was developed. the method consisted of incubating cell extracts with 3h-decarboxylated s-adenosylmethionine and sodium cyanoborohydride in the presence of a spermidine synthase inhibitor. under these conditions, s-adenosylmethionine decarboxylase was labeled specifically and stoichiometrically. this procedure was used (a) to establish that the subunit molecular weight of s-adenosylmethionine decarboxylase ... | 1985 | 3902085 |
| myocardial dysfunction in a nonlethal, nonshock model of chronic endotoxemia. | escherichia coli endotoxin (et) was administered to adult rats by continuous iv infusion from a subcutaneously implanted osmotic pump (alzet). myocardial function was assessed after 6 and 30 hr of et infusion and compared with control rats which received a saline infusion and were fasted to match the anorexia of et rats. cardiac output (co) and coronary blood flow, measured by the radiolabeled microsphere method, and mean arterial blood pressure, heart rate, total peripheral resistance, and stro ... | 1985 | 3902271 |
| plasma catecholamines during endotoxin infusion in conscious unrestrained rats: effects of adrenal demedullation and/or guanethidine treatment. | we have examined the effect of e coli endotoxin infusion (41 micrograms kg-1 min-1 iv for 4 h) on plasma concentrations of epinephrine (e), norepinephrine (ne), and dopamine (da) in the conscious unrestrained rat. saline infusion did not change catecholamine concentrations from the preinfusion values of 230.8 +/- 32.9 pg ml-1 (e), 456.8 +/- 104.9 pg ml-1 (ne), and 49.0 +/- 19.9 pg ml-1 (da). endotoxin produced marked elevations in all three catecholamines. at 1 h, the plasma concentrations were ... | 1985 | 3902274 |
| decreased clearance of escherichia coli from the bile in rats with obstructive jaundice. | clearance of escherichia coli from the bile was studied in 4 normal sprague-dawley rats and 4 rats with 3 weeks of obstruction of the common bile duct. 125i-radiolabelled heat-killed e. coli were injected into the common bile duct and the radioactivity of the bile monitored for 2 h. the radioactivity declined exponentially during the first 10 min. bile samples collected from 2 to 15 min after injection showed higher amounts of radioactivity in all rats with biliary obstruction than in the normal ... | 1985 | 3902481 |
| in vitro adherence of escherichia coli to endometrial epithelial cells of rats and influence of estradiol. | the influence of ovarian hormones on the adhesion of escherichia coli to endometrial epithelial cells was investigated in an in vitro system. endometrial cells liberated by collagenase from rat uteri were used. optimal test conditions were obtained when 5 x 10(8) e. coli bacteria were added to 10(5) epithelial cells and incubated for 60 min. the adhesion of the organisms was inhibited by the addition of either mannose or alpha-methyl-d-mannopyranoside. when epithelial cells collected from uteri ... | 1985 | 3902646 |
| studies on immunity against escherichia coli k13 with monoclonal anti-k13 and anti-anti-k13. | the structural basis for the cross-reactivity between the escherichia coli k13, k20 and k23 capsular polysaccharides is the----)-beta-ribofuranosyl-(1----7)-beta-2-keto-3-deoxyoctonate polymer. monoclonal antibodies against e. coli k13 which require o-acetyl-2-keto-3-deoxyoctonate for binding were further investigated. such antibodies, of both the igg and the igm isotype, opsonized e. coli k13 in vitro and protected against intraperitoneal infection in mice as well as ascending pyelonephritis in ... | 1985 | 3902658 |
| effects of endotoxin and sodium salicylate on the preoptic thermosensitive neurons in tissue slices. | effects of e. coli endotoxin and sodium salicylate (sal) on single-unit activity of thermosensitive neurons recorded in slices of preoptic and anterior hypothalamic area (po/ah) were studied in vitro. perfusion of endotoxin-containing krebs-ringer's solution or local application of endotoxin in the immediate vicinity of recording neurons decreased and increased the firing rate of 31 of 34 warm-sensitive neurons and a cold-sensitive neuron, but had no effect on the majority of thermally insensiti ... | 1985 | 3904925 |
| comparison of gentamicin and kanamycin alone and in combination with ampicillin in experimental escherichia coli bacteremia and meningitis. | the conventional antimicrobial therapy of gram-negative infection in the newborn is the combination of ampicillin and an aminoglycoside, usually gentamicin or kanamycin. although gentamicin and kanamycin have been used interchangeably, efficacies of the two drugs have not been carefully compared. in addition, the contribution of ampicillin to the outcome of neonatal gram-negative meningitis is controversial. we evaluated the activity of gentamicin and kanamycin alone and in combinations with amp ... | 1985 | 3906546 |
| synthesis and expression of a human growth hormone (somatotropin) gene mutated to change cysteine-165 to alanine. | we have mutated a synthetic human growth hormone (hgh) gene specifically at the codon for cys-165 to a codon for ala by replacement of synthetic deoxyoligonucleotides corresponding to this site. this modification prevented the formation of a disulfide bond between cys-53 and cys-165 in the hgh molecule. this mutated protein, [ala165]hgh was expressed at the same level as the intact hgh, 4 x 10(5) molecules per cell under the control of the tryptophan promoter in escherichia coli, and retained si ... | 1985 | 3908100 |
| human apolipoprotein e expression in escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein e. | human apolipoprotein e (apoe) was produced in escherichia coli by transforming cells with an expression vector containing a reconstructed apoe cdna, a lambda pl promoter regulated by the thermolabile ci repressor, and a ribosomal binding site derived from the lambda cii or the e. coli beta-lactamase gene. transformed cells induced at 42 degrees c for short periods of time (less than 20 min) produced apoe, which accumulated in the cells at levels of approximately equal to 1% of the total soluble ... | 1985 | 3909150 |
| studies of the repair of o6-alkylguanine and o4-alkylthymine in dna by alkyltransferases from mammalian cells and bacteria. | o6-methylguanine in dna is repaired by the action of a protein termed o6-alkylguanine-dna alkyltransferase (at) which transfers the methyl group to a cysteine residue in its own sequence. since the cysteine which is methylated is not regenerated rapidly, if at all, the capacity for repair of o6-methylguanine is limited by the number of molecules of the at available within the cell. the level and inducibility of the at differed greatly in different mammalian cell types and species with the highes ... | 1985 | 3910413 |
| [ccl4 resistance of the liver after endotoxin and prodigiozan stimulation]. | | 1985 | 3912716 |
| [bacteriologic, histologic and functional studies of the kidney in 5 days' therapy of experimental e. coli pyelonephritis with gentamycin. comparison with 9 days' therapy]. | short-term antibiotic treatment is recommended in infections of the lower urinary tract but its effectiveness is questioned in the upper urinary tract infections. we compared a 5 and a 9 day treatment of experimental e. coli 022 pyelonephritis after unilateral nephrectomy in 127 mal wistar rats. we used 9 mg gentamycin per kg b.w. twice daily. i131 hippurat excretion was not decreased during the 5 day treatment but was only temporarily diminished during the 9 day treatment. histologically the se ... | 1985 | 3913218 |
| [cooperation between gentamycin and netilmicin in sub-inhibitor doses and defensive mechanisms of the host]. | | 1985 | 3913616 |
| in vivo aspects of urogastrone-epidermal growth factor. | recent evidence indicates that human urogastrone-epidermal growth factor originates in submandibular and brunner's glands and that serum levels are low (less than 1 ng ml-1). it occurs in many secreted fluids to a much greater extent and many tissues are thus exposed to concentrations greater than 100 ng ml-1. rapid actions in vivo of uro-egf include the ability to inhibit gastric acid secretion at low doses (250 ng kg h-1 in humans) and to provide cytoprotective effects against ulcerogenic agen ... | 1985 | 3914989 |
| nitrosatable precursors of mutagens in vegetables and soy sauce. | nitrosatable precursors of mutagens that show mutagenicity to salmonella typhimurium ta100 without s9 mix after treatment with nitrite at ph 3 were found in various foods. from chinese cabbage, three indole compounds, indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile, and 4-methoxyindole-3-aldehyde, were identified as mutagen precursors. 1-methylindole and 2-methylindole, which are present in cigarette smoke showed strong mutagen precursor activity. escherichia coli wp2 uvra/pkm101 is more s ... | 1985 | 3916206 |
| expression in mammalian cells of the diaminopimelic acid decarboxylase of escherichia coli permits cell growth in lysine-free medium. | the lysa gene of escherichia coli encodes for a diaminopimelic acid decarboxylase (ec 4.1.1.20) which allows the conversion of diaminopimelic acid into lysine in bacteria. it has been cloned in an eukaryotic expression vector containing upstream the sv40 early promoting sequence, and downstream mouse alpha-globin maturating sequences. the recombinant plasmid psb99 (4800 base pairs) has been introduced into several mammalian cell lines by cotransfection with a second selectable marker i.e. the po ... | 1985 | 3917917 |
| dramatic changes in blood gases that are unrelated to arterial ph or cerebral oxygen delivery during endotoxin shock in conscious rats. | in preliminary studies we demonstrated an effect of endotoxin on arterial blood gases that appeared to be related to the dose of endotoxin used and unrelated to changes in arterial ph. in the present study we tested the hypothesis that these changes in blood gases result from decreased oxygen delivery to central respiratory control areas. po2 significantly rose from a pre-endotoxin value of 91.4 +/- 1.5 (mean +/- sem) to 97.5 +/- 1.7, 104.0 +/- 0.8, and 108.4 +/- 0.9 at 10, 30, and 60 minutes, r ... | 1985 | 3919958 |
| metabolic and cardiovascular effects of endotoxin infusion in conscious unrestrained rats: effects of methylprednisolone and bw755c. | infusion of escherichia coli endotoxin (41.7 micrograms kg-1 min-1 i.v. for 4 h; 10 mg kg-1 total) in conscious unrestrained rats produced an increase in heart rate and a gradual decrease in arterial blood pressure. initially plasma glucose was transiently elevated but fell to hypoglycaemic values in the 1-2 h before death. there was a marked elevation in the plasma concentrations of lactate, thromboxane b2, and 6-keto prostaglandin f1 alpha, (pgf1 alpha). methylprednisolone treatment (two doses ... | 1985 | 3919962 |
| alpha-hydroxylation and mutagenicity of unsymmetrical n-nitrosodialkylamines with a butyl group. | in order to compare the extents of metabolic alpha-hydroxylation of the two alkyl groups in unsymmetrical n-nitrosodialkylamines, n-nitroso-n-alkylbutylamines (alkyl = methyl, ethyl, propyl, butyl, and amyl) were incubated with liver microsomes prepared from phenobarbital- or polychlorinated biphenyl (pcb)-treated and untreated rats, and aldehydes produced by the alpha-hydroxylation were determined quantitatively. although an increased production of aldehydes was observed with the induced micros ... | 1985 | 3922836 |
| stimulation of intestinal adenylate cyclase by cholera toxin in malnourished rats. | the stimulation of intestinal adenylate cyclase by cholera toxin (ct) was studied in normal and malnourished rats 4 to 24 hr after a 30-min incubation of intestinal loops with the toxin. whereas in control rats the enzyme activity returned to basal levels after 12 hr of incubation, in malnourished rats the activity of the enzyme remained significantly elevated even after 24 hr of the initial incubation. malnourished animals had a reduced turnover rate of intestinal cells as determined by thymidi ... | 1985 | 3931085 |
| treatment of alveolar macrophages with cytochalasin d inhibits uptake and subsequent growth of legionella pneumophila. | legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin d per ml. attachment was not inhibited by cytochalasin d. no extracellular multiplication occurred when l. pneumophila were in close proximity to viable functional macrophages or even when the bacteria were attached to plasma membranes of the macrophages. nonopsonized l. pneumophila were avidly pha ... | 1986 | 3941000 |
| mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal microflora. | to investigate the modifying role of intestinal microflora in the metabolism of chemical carcinogens in vivo, we subjected bile from fischer rats treated per os with chemical carcinogens and related compounds to a mutagenicity assay in the presence and absence of a cell-free extract from human feces. a mixture of the bile sample and potassium phosphate buffer was incubated in the presence or absence of human cell-free fecal extract and then further incubated with a bacterial suspension of salmon ... | 1985 | 3982438 |
| biological and immunological characteristics of 125i-4tyr and -18tyr escherichia coli heat-stable enterotoxin species purified by high-performance liquid chromatography. | a rapid procedure for the separation of escherichia coli heat-stable enterotoxin iodination products is described. a two-step procedure using reverse-phase chromatography results in the separation of native, noniodinated heat-stable enterotoxins from several distinctly iodinated species which differ in their ability to bind to brush border receptors. sequence analysis of the radioligands demonstrated mutually exclusive labeling at the 4tyr and 18tyr residues. these tracers have been immunologica ... | 1985 | 4041144 |
| role of alveolar macrophages in endotoxin-induced neutrophilic alveolitis in rats. | although bacterial endotoxins have potent effects on blood monocytes and tissue macrophages, the role of alveolar macrophages in regulating intrapulmonary neutrophil traffic following endotoxemia has not been studied previously. we have previously reported that a single intraperitoneal injection of endotoxin from escherichia coli serotype 055b5 causes acute lung inflammation by neutrophils (pmn) in rats. the factors which influence the migration of pmn in the lung in this model are unknown. to d ... | 1985 | 4049362 |
| extent of formation of o4-methylthymidine in calf thymus dna methylated by n-methyl-n-nitrosourea and lack of repair of this product by rat liver o6-alkylguanine-dna-alkyltransferase. | calf thymus dna was methylated by reaction with n-[3h]-methyl-n-nitrosourea and the content of o6-methyldeoxyguanosine, 3-methylthymidine and o4-methylthymidine was determined. it was found that o4-methylthymidine represented only 0.06 +/- 0.02% of the total methylation and that the ratio of o6-methyldeoxyguanosine:o4-methylthymidine was 126 +/- 31. 3-methylthymidine represented only 0.05 +/- 0.01% of the total radioactivity and the ratio of o6-methyldeoxyguanosine:3-methylthymidine was 171 +/- ... | 1985 | 4053281 |
| [study of mouse or rat livers perfused "in vitro" with blood containing bacterial dna]. | | 1970 | 4098015 |
| antigenic relationships in mammalian dna polymerase. | rabbit antibody was prepared against a high-molecular-weight dna polymerase purified from the soluble fraction of calf thymus gland. this antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight dna polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse l cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone ... | 1972 | 4110484 |
| mechanisms of steroid protection in septic shock. | | 1972 | 4113486 |
| a technique for ultracryotomy of cell suspensions and tissues. | ultracryotomy of fixed tissue has been investigated for a number of years but, so far, success has been limited for several reasons. the simple technique herein reported allows the ultracryotomy not only of a variety of tissues but also of single cells in suspension, with a preservation and visualization of ultrastructural detail at least equivalent to that obtained with conventional embedding procedures. in this technique, sucrose is infused into glutaraldehyde-fixed tissue pieces before freezi ... | 1973 | 4121290 |
| specific serological relationships among partially purified dna polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and avian cells. | specific serological relationships were found among the partially purified dna polymerases of the two groups of avian viruses whose virions contain rna and a dna polymerase-the avian leukosis-sarcoma viruses and the reticuloendotheliosis viruses-and three avian species which are natural hosts for these viruses: chickens, turkeys, and pekin ducks. no relationships were found to dna polymerases of hela cells or escherichia coli. these results are consistent with the hypothesis that rna viruses wit ... | 1974 | 4132917 |
| [study of the polyribosomes of the spleen of animals immunized with e. coli beta-galactosidase]. | | 1973 | 4134833 |
| increased serum immunoglobulin levels following portacaval shunt in the normal rat. | normal rats subjected to end-to-side portacaval shunt showed decreased survival and weight gain, a progressive fall in serum albumin and reciprocal rise in serum gamma globulin when compared with sham-operated controls for 12 weeks. antibacterial lipopolysaccharide antibody was detected in significant titre at the sixth and twelfth weeks. it is suggested that the elevated levels of gamma globulin and reversal of albumin/globulin ratios noted in these animals may represent an immune response to b ... | 1974 | 4136811 |
| a nicotinamide-adenine dinucleotide assay utilizing liver alcohol dehydrogenase. | | 1974 | 4152749 |
| [models of urinary infection]. | | 1974 | 4154600 |
| [humoral and cellular factors of immunological defense against infection exemplified by estrogen-facilitated pyelonephritis in rats]. | | 1974 | 4154603 |
| [experimental evaluation of the new cephalosporin antibiotic cephacetrile]. | | 1974 | 4154769 |
| experimental pyelonephritis. a light and electron microscopic study of the periodic acid-schiff positive interstitial cell. | | 1969 | 4194271 |
| [fundamental and clinical evaluation of lividomycin]. | | 1972 | 4198943 |
| dissociation of eukaryotic ribosomes by purified initiation factor eif-3. | | 1973 | 4200395 |
| [experimental cystitis, a modell of chronic bacteriuria in rats (author's transl)]. | | 1973 | 4203397 |
| [some characteristics of the transcription process in the chromatin of liver cells in chemical carcinogenesis]. | | 1973 | 4205440 |
| aminoacyl transfer ribonucleic acid synthesis in toluene-treated liver cells. | | 1974 | 4211789 |
| rapid differentiation of certain bacteria in mixed populations by gas-liquid chromatography. | | 1973 | 4215243 |
| [appearance of antibodies to one of the general antigens of microorganisms of the intestinal group after administration to animals of carcinogenic substances and in anacid gastritis in man]. | | 1974 | 4216001 |
| biological effects of gravitational fields. | | 1974 | 4216044 |
| [study of a new synthetic antibacterial agent: 8-ethyl-5-oxo-2-piperaziny 5,8-dihydro-(2,3-d)pyrimidine-6-carboxylic acid (pipemidic acid)]. | | 1974 | 4219332 |
| influence of polyanions and polycationic complexes of metals on cells and cellular organelles. | | 1967 | 4233806 |
| experimental infections of the kidneys and urinary tract in pregnant rats. | | 1969 | 4245127 |
| [effect of aristolochic acid on leukocyte phagocytosis]. | | 1970 | 4249811 |
| [trna methylases]. | | 1972 | 4262108 |
| genetic control of thymus-derived cell function. iv. mitogen responsiveness and mixed lymphocyte reactivity of thymus cells and lymph node cells from lewis and brown norway rats. | | 1973 | 4270394 |
| rna fractionation on reversed-phase columns. | | 1973 | 4271855 |
| the development of the flora of the alimentary tract in young animals. | | 1965 | 4285022 |
| the inhibition of enzymes by beryllium. | 1. the action of beryllium on the following enzymes has been examined: alkaline phosphatase (escherichia coli and kidney), acid phosphatase, phosphoprotein phosphatase, apyrase (potato), adenosine triphosphatase (liver nuclei, liver mitochondria, brain microsomes), glucose 6-phosphatase, polysaccharide phosphorylases a and b, phosphoglucomutase, hexokinase, phosphoglyceromutase, ribonuclease, a-esterase (rabbit serum), cholinesterase (horse serum), chymotrypsin. alkaline phosphatase and phosphog ... | 1966 | 4287187 |