| abnormal mitoferrin-1 expression in patients with erythropoietic protoporphyria. | objective: most patients with erythropoietic protoporphyria have deficient ferrochelatase (fech) activity due to changes in fech dna. we evaluated seven patients with erythropoietic protoporphyria phenotype in whom abnormalities of fech dna were not found by conventional analysis. the major focus was mitoferrin-1 (mfrn1), the mitochondrial transporter of fe used for heme formation by fech and for 2fe2s cluster synthesis, which is critical to fech activity/stability. materials and methods: four p ... | 2011 | 21627978 |
| yapsin 1 immunoreactivity in alpha cells of human pancreatic islets: implications for the processing of human proglucagon by mammalian aspartic proteases. | yapsin 1 is an aspartic protease from saccharomyces cerevisiae and belongs to a class of aspartic proteases that demonstrate specificity for basic amino acids. it is capable of processing prohormone substrates at specific basic residue cleavage sites, similar to that of the prohormone convertases, to generate bioactive peptide hormones. an antibody raised against yapsin 1 was previously shown to immuno-stain endocrine cells of rat pituitary and brain as well as lysates from bovine pituitary secr ... | 2011 | 21632904 |
| over-expression of human calnexin in yeast improves measles surface glycoprotein solubility. | the limitations of high-level expression of virus surface proteins in yeast are not well understood. the inefficiency of yeast to produce active human virus surface glycoproteins, as well as other mammalian glycoproteins, is usually explained by the inefficient folding of the glycoprotein into its characteristic and functional three-dimensional structure from a random coil. the endoplasmic reticulum is a highly versatile protein factory that is equipped with chaperones and folding enzymes essent ... | 2011 | 21635688 |
| binding site prediction for protein-protein interactions and novel motif discovery using re-occurring polypeptide sequences. | abstract: background: while there are many methods for predicting protein-protein interaction, very few can determine the specific site of interaction on each protein. characterization of the specific sequence regions mediating interaction (binding sites) is crucial for an understanding of cellular pathways. experimental methods often report false binding sites due to experimental limitations, while computational methods tend to require data which is not available at the proteome-scale. here we ... | 2011 | 21635751 |
| structure of the guanylyltransferase domain of human mrna capping enzyme. | the enzyme guanylyltransferase (gtase) plays a central role in the three-step catalytic process of adding an (m7)gpppn cap cotranscriptionally to nascent mrna (pre-mrnas). the 5'-mrna capping process is functionally and evolutionarily conserved from unicellular organisms to human. however, the gtases from viruses and yeast have low amino acid sequence identity (∼25%) with gtases from mammals that, in contrast, are highly conserved (∼98%). we have defined by limited proteolysis of human capping e ... | 2011 | 21636784 |
| field testing of different chemical combinations as odour baits for trapping wild mosquitoes in the gambia. | odour baited traps have potential use in population surveillance of insect vectors of disease, and in some cases for vector population reduction. established attractants for human host-seeking mosquitoes include a combination of co(2) with l-lactic acid and ammonia, on top of which additional candidate compounds are being tested. in this field study in rural gambia, using latin square experiments with thorough randomization and replication, we tested nine different leading candidate combinations ... | 2011 | 21637337 |
| an acidic loop and cognate phosphorylation sites define a molecular switch that modulates ubiquitin charging activity in cdc34-like enzymes. | e2 ubiquitin-conjugating enzymes are crucial mediators of protein ubiquitination, which strongly influence the ultimate fate of the target substrates. recently, it has been shown that the activity of several enzymes of the ubiquitination pathway is finely tuned by phosphorylation, an ubiquitous mechanism for cellular regulation, which modulates protein conformation. in this contribution, we provide the first rationale, at the molecular level, of the regulatory mechanism mediated by casein kinase ... | 2011 | 21637798 |
| gene network landscape of the ciliate tetrahymena thermophila. | genome-wide expression data of gene microarrays can be used to infer gene networks. at a cellular level, a gene network provides a picture of the modules in which genes are densely connected, and of the hub genes, which are highly connected with other genes. a gene network is useful to identify the genes involved in the same pathway, in a protein complex or that are co-regulated. in this study, we used different methods to find gene networks in the ciliate tetrahymena thermophila, and describe s ... | 2011 | 21637855 |
| involvement of a toxoplasma gondii chromatin remodeling complex ortholog in developmental regulation. | the asexual cycle of the parasite toxoplasma gondii has two developmental stages: a rapidly replicating form called a tachyzoite and a slow growing cyst form called a bradyzoite. while the importance of atp-independent histone modifications for gene regulation in t. gondii have been demonstrated, atp-dependent chromatin remodeling pathways have not been examined. in this study we characterized c9, an insertional mutant showing reduced expression of bradyzoite differentiation marker bag1, in cult ... | 2011 | 21655329 |
| calcium signaling components in the human pathogen: cryptococcus neoformans. | calcium signaling through calmodulin and the phosphatase calcineurin are required for key events of the biology of the human pathogen cryptococcus neoformans, including mating, morphogenesis, growth at 37°c and virulence. in a recent work we described the functional characterization of a new component of this calcium signaling network: the vacuolar calcium exchanger vcx1. this transporter is involved in calcium tolerance and virulence in c. neoformans. two other uncharacterized calcium transport ... | 2011 | 21655435 |
| the over-expression of the ╬▓2 catalytic subunit of the proteasome decreases homologous recombination and impairs dna double-strand break repair in human cells. | by a human cdna library screening, we have previously identified two sequences coding two different catalytic subunits of the proteasome which increase homologous recombination (hr) when overexpressed in the yeast saccharomyces cerevisiae. here, we investigated the effect of proteasome on spontaneous hr and dna repair in human cells. to determine if the proteasome has a role in the occurrence of spontaneous hr in human cells, we overexpressed the ╬▓2 subunit of the proteasome in hela cells and d ... | 2011 | 21660142 |
| in vivo interaction of the hepatitis delta virus small antigen with the elav-like protein hur. | the small and large delta antigens (s-hdag and l-hdag, respectively) represent two forms of the only protein encoded by the hepatitis delta virus (hdv) rna genome. consequently, hdv relies, at a large extent, on the host cell machinery for replication and transcription. until now, only a limited number of cellular proteins were identified as s-hdag or l-hdag partners being involved in the modulation of the virus life cycle. in an attempt to identify cellular s-hdag-binding proteins we made use o ... | 2011 | 21660185 |
| model organisms reveal insight into human neurodegenerative disease: ataxin-2 intermediate-length polyglutamine expansions are a risk factor for als. | model organisms include yeast saccromyces cerevisae and fly drosophila melanogaster. these systems have powerful genetic approaches, as well as highly conserved pathways, both for normal function and disease. here, we review and highlight how we applied these systems to provide mechanistic insight into the toxicity of tdp-43. tdp-43 accumulates in pathological aggregates in als and about half of ftd. yeast and fly studies revealed an interaction with the counterparts of human ataxin-2, a gene wh ... | 2011 | 21660502 |
| do cancer proteins really interact strongly in the human protein-protein interaction network? | protein-protein interaction (ppi) network analysis has been widely applied in the investigation of the mechanisms of diseases, especially cancer. recent studies revealed that cancer proteins tend to interact more strongly than other categories of proteins, even essential proteins, in the human interactome. however, it remains unclear whether this observation was introduced by the bias towards more cancer studies in humans. here, we examined this important issue by uniquely comparing network char ... | 2011 | 21666777 |
| osbp-related protein 7 interacts with gate-16 and negatively regulates gs28 protein stability. | orp7 is a member of oxysterol-binding protein (osbp) family, the function of which has remained obscure. in this study, we identified by yeast two-hybrid screening an interaction partner of orp7, gate-16, which (i) regulates golgi snare of 28kda (gs28) function and stability, and (ii) plays a role in autophagosome biogenesis. the interaction was confirmed by bimolecular fluorescence complementation (bifc) assay in living cells. the interacting regions were delineated within aa 1-142 of orp7 and ... | 2011 | 21669198 |
| insights into the structure of the ccr4-not complex by electron microscopy. | the ccr4-not complex is a deadenylation complex, which plays a major role for mrna stability. the complex is conserved from yeast to human and consists of nine proteins not1-not5, ccr4, caf1, caf40 and caf130. we have successfully isolated the complex using a protein a tag on not1, followed by cross-linking on a glycerol gradient. all components of the complex were identified by mass spectrometry. electron microscopy of negatively stained particles followed by image reconstruction revealed an l- ... | 2011 | 21669201 |
| substitution patterns are gc-biased in divergent sequences across the metazoans. | the fastest-evolving regions in the human and chimpanzee genomes show a remarkable excess of weak (a,t) to strong (g,c) nucleotide substitutions since divergence from their common ancestor. we investigated the phylogenetic extent and possible causes of this weak to strong (w ôåæ s) bias in divergent sequences (bds) using recently sequenced genomes and recombination maps from eight trios of eukaryotic species. to quantify evidence for bds, we inferred substitution histories using an efficient max ... | 2011 | 21670083 |
| fission yeast homologs of human xpc and csb, rhp41 and rhp26, are involved in transcription-coupled repair of methyl methanesulfonate-induced dna damage. | methyl methanesulfonate (mms) methylates nitrogen atoms in purines, and predominantly produces 7-methylguanine and 3-methyladenine (3-mea). previously, we showed that base excision repair (ber) and nucleotide excision repair (ner) synergistically function to repair mms-induced dna damage in the fission yeast schizosaccharomyces pombe. here, we studied the roles of ner components in repair of 3-mea and ber intermediates such as the ap site and single strand breaks. mutants of rhp41 (xpc homolog) ... | 2011 | 21670547 |
| p53 transactivation and the impact of mutations, cofactors and small molecules using a simplified yeast-based screening system. | the p53 tumor suppressor, which is altered in most cancers, is a sequence-specific transcription factor that is able to modulate the expression of many target genes and influence a variety of cellular pathways. inactivation of the p53 pathway in cancer frequently occurs through the expression of mutant p53 protein. in tumors that retain wild type p53, the pathway can be altered by upstream modulators, particularly the p53 negative regulators mdm2 and mdm4. | 2011 | 21674059 |
| plant ornithine decarboxylase is not post-transcriptionally feedback regulated by polyamines but can interact with a cytosolic ribosomal protein s15 polypeptide. | the formation of putrescine by ornithine decarboxylase (odc) is a key regulatory step in polyamine biosynthesis in metazoa and fungi. excess polyamines post-transcriptionally induce the synthesis of a unique non-competitive protein inhibitor of odc, termed antizyme. binding of antizyme to an odc monomer subunit results in enzymatic inhibition, rapid ubiquitin-independent degradation of odc by the 26s proteasome and recycling of antizyme. plants possess an additional route for synthesizing putres ... | 2011 | 21814791 |
| detection of antibodies against paracoccidioides brasiliensis melanin: in vitro and in vivo studies during infection. | several cell-wall constituents, including melanins or melanin-like compounds, have been implicated in the pathogenesis of a wide variety of microbial diseases caused by diverse species of pathogenic bacteria, fungi and helminthes. among these microorganisms, the dimorphic fungal pathogen paracoccidioides brasiliensis produces melanin in its conidial and yeast forms. in the present study, melanin particles from p. brasiliensis were injected into balb/c mice in order to produce monoclonal antibodi ... | 2011 | 21813659 |
| human cabin1 is a functional member of the human hira/ubn1/asf1a histone h3.3 chaperone complex. | the mammalian hira/ubn1/asf1a complex is a histone chaperone complex that is conserved from yeast to humans. this complex preferentially deposits the histone variant h3.3 into chromatin in a dna replication-independent manner, and is implicated in diverse chromatin regulatory events from gene activation to heterochromatinization. in yeast, the orthologous complex consists of three hir proteins (hir1p, hir2p and hir3p), hpc2p and asf1p. yeast hir3p has weak homology to, cabin1, a fourth member of ... | 2011 | 21807893 |
| phospholipid scramblase 1 mediates hepatitis c virus entry into host cells. | hepatitis c virus (hcv) infects human hepatocytes through several host factors. however, other prerequisite factors for viral entry remain to be identified. using a yeast two-hybrid screen, we found that human phospholipid scramblase 1 interacts with hcv envelope proteins e1 and e2. these physical interactions were confirmed by co-immunoprecipitation and gst pull-down assays. knocking down the expression of plscr1 inhibited the entry of hcv pseudoparticles. moreover, plscr1 was required for the ... | 2011 | 21806988 |
| the cellular dexd/h-box rna-helicases uap56 and urh49 exhibit a crm1-independent nucleocytoplasmic shuttling activity. | cellular dexd/h-box rna-helicases perform essential functions during mrna biogenesis. the closely related human proteins uap56 and urh49 are members of this protein family and play an essential role for cellular mrna export by recruiting the adaptor protein ref to spliced and unspliced mrnas. in order to gain insight into their mode of action, we aimed to characterize these rna-helicases in more detail. here, we demonstrate that uap56 and urh49 exhibit an intrinsic crm1-independent nucleocytopla ... | 2011 | 21799930 |
| ddx60, a dexd/h box helicase, is a novel antiviral factor promoting rig-i-like receptor-mediated signaling. | the cytoplasmic viral rna sensors rig-i and mda5 are important for the production of type i interferon and other inflammatory cytokines. ddx60 is an uncharacterized dexd/h box rna helicase similar to s. cerevisiae ski2, a cofactor of rna exosome, a protein complex required for the integrity of cytoplasmic rna. the expression of ddx60 increases after viral infection, and the protein localizes at cytoplasmic region. after viral infection, the ddx60 protein binds to endogenous rig-i protein. the pr ... | 2011 | 21791617 |
| papd5, a noncanonical poly(a) polymerase with an unusual rna-binding motif. | papd5 is one of the seven members of the family of noncanonical poly(a) polymerases in human cells. papd5 was shown to polyadenylate aberrant pre-ribosomal rnas in vivo, similar to degradation-mediating polyadenylation by the noncanonical poly(a) polymerase trf4p in yeast. papd5 has been reported to be also involved in the uridylation-dependent degradation of histone mrnas. to test whether papd5 indeed catalyzes adenylation as well as uridylation of rna substrates, we analyzed the in vitro prope ... | 2011 | 21788334 |
| identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein. | the vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. pdz domains, composed of 80-100 amino acid residues, are an important class of interaction motif. among the pdz-containing proteins, glutaminase interacting protein (gip), also known as tax interacting protein tip-1, is unique in being composed almost exclusively of a single pdz domain. gip has important roles in cellular signaling, prot ... | 2011 | 21787750 |
| turnover of atp synthase subunits in f(1)-depleted hela and yeast cells. | mitochondrial translation of the saccharomyces cerevisiae atp6p subunit of f(1)-f(0) atp synthase is regulated by the f(1) atpase. here we show normal expression of atp6p in hela cells depleted of the f(1) ß subunit. instead of being translationally down-regulated, hela cells lacking f(1) degrade atp6p, thereby preventing proton leakage across the inner membrane. mammalian mitochondria also differ in the way they minimize the harmful effect of unassembled f(1) a subunit. while yeast mutants lack ... | 2011 | 21784071 |
| [activity of macrolides and fluoroquinolones against intracellular legionella pneumophila]. | to evaluate the activity of macrolides and fluoroquinolones against legionella pneumophila by intracellular susceptibility testing. | 2011 | 21781510 |
| identification of an evolutionary conserved family of inorganic polyphosphate endopolyphosphatases. | inorganic polyphosphate (poly-p) consists of just a chain of phosphate groups linked by high-energy bonds. it is found in every organism and is implicated in a wide variety of cellular processes, e.g. phosphate storage, blood coagulation, pathogenicity. its metabolism has been studied mainly in bacteria, while remaining largely uncharacterized in eukaryotes. it has recently been suggested that poly-p metabolism is connected to that of highly phosphorylated inositol species (inositol pyrophosphat ... | 2011 | 21775424 |
| [the effects of tata-box in cyc1 promoter on the reporter gene regulated by ere in the recombinant yeast cell]. | the complementary oligonucleotides, each with two consensus estrogen response element (ere)-sequences and 5'-hind iii and 3'-sph i sticky ends were artificially synthesized. a solution with both the complementary dna sequences was heated to 95'c and cooled down to room temperature to form double strand dna (dsdna). the set was cloned into the corresponding sites of cyc1 promoter of the pere-cyc-yegfp to yield pere-cycalpha-yegfp vector. the two different reporter vectors, pere-cyc-yegfp and pere ... | 2011 | 21774222 |
| effect of phyllanthus emblica linn. on candida adhesion to oral epithelium and denture acrylic. | to investigate the effect of phyllanthus emblica (p. emblica) linn. ethanolic extract on the adhesion of candida albicans (c. albicans) to human buccal epithelial cells (becs) and denture acrylic surfaces. | 2011 | 21771413 |
| type ix collagen interacts with fibronectin providing an important molecular bridge in articular cartilage. | type ix collagen is covalently bound to the surface of type ii collagen fibrils within the cartilage ecm. the n-terminal, globular non-collagenous domain (nc4) of the a1(ix) chain protrudes away from the surface of the fibrils into the surrounding matrix and is available for molecular interactions. to define these interactions we used the nc4 domain in a yeast two-hybrid screen of a human chondrocyte cdna library. 73% of the interacting clones encoded fibronectin. the interaction was confirmed u ... | 2011 | 21768108 |
| human fatty acid transport protein 2a/very long chain acyl coa synthetase 1 (fatp2a/acsvl1) has a preference in mediating the channeling of exogenous n-3 fatty acids into phosphatidylinositol. | the trafficking of fatty acids across the membrane and into downstream metabolic pathways requires their activation to coa thioesters. members of the fatty acid transport protein/very long chain acyl coa synthetase (fatp/acsvl) family are emerging as key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. we have expressed two naturally occurring splice variants of fatp2 (acsvl1) in yeast and 293t-rex cells and addressed their roles in f ... | 2011 | 21768100 |
| sirtuins, bioageing, and cancer. | the sirtuins are a family of orthologues of yeast sir2 found in a wide range of organisms from bacteria to man. they display a high degree of conservation between species, in both sequence and function, indicative of their key biochemical roles. sirtuins are heavily implicated in cell cycle, cell division, transcription regulation, and metabolism, which places the various family members at critical junctures in cellular metabolism. typically, sirtuins have been implicated in the preservation of ... | 2011 | 21766030 |
| characterization of yipf3 and yipf4, cis-golgi localizing yip domain family proteins. | the yip1 domain family (yipf) proteins are homologues of yeast yip1p and yif1p, which are proposed to function in er to golgi transport. here, we report the characterization of yipf3 and yipf4, homologues of human yif1p and yip1p, respectively. immunofluorescence and immuno-electron microscopy showed that both yipf3 and yipf4 are clearly concentrated in the cis-golgi. while yipf4 was detected as a single mobility form consistent with its predicted molecular weight, three different mobility forms ... | 2011 | 21757827 |
| cell surface display of functional human mhc class ii proteins: yeast display versus insect cell display. | reliable and robust systems for engineering functional major histocompatibility complex class ii (mhcii) proteins have proved elusive. availability of such systems would enable the engineering of peptide-mhcii (pmhcii) complexes for therapeutic and diagnostic applications. in this paper, we have developed a system based on insect cell surface display that allows functional expression of heterodimeric dr2 molecules with or without a covalently bound human myelin basic protein (mbp) peptide, which ... | 2011 | 21752831 |
| the inhibitor of growth protein 5 (ing5) depends on inca1 as a co-factor for its antiproliferative effects. | the proteins of the inhibitor of growth (ing) family are involved in multiple cellular functions such as cell cycle regulation, apoptosis, and chromatin remodeling. for ing5, its actual role in growth suppression and the necessary partners are not known. in a yeast-two-hybrid approach with human bone marrow derived cdna, we identified ing5 as well as several other proteins as interaction partners of inhibitor of cyclin a1 (inca1) that we previously characterized as a novel interaction partner of ... | 2011 | 21750715 |
| ploidy influences cellular responses to gross chromosomal rearrangements in saccharomyces cerevisiae. | abstract: | 2011 | 21711526 |
| overexpression of a homogeneous oligosaccharide with (13)c labeling by genetically engineered yeast strain. | this report describes a novel method for overexpression of (13)c-labeled oligosaccharides using genetically engineered saccharomyces cerevisiae cells, in which a homogeneous high-mannose-type oligosaccharide accumulates because of deletions of genes encoding three enzymes involved in the processing pathway of asparagine-linked oligosaccharides in the golgi complex. using uniformly (13)c-labeled glucose as the sole carbon source in the culture medium of these engineered yeast cells, high yields o ... | 2011 | 21698488 |
| desiccation tolerance of prokaryotes: application of principles to human cells. | the loss of water from cells is a stress that was likely imposed very early in evolution. an understanding of the sensitivity or tolerance of cells to depletion of intracellular water is relevant to the study of quiescence, longevity and aging, because one consequence of air-drying is full metabolic arrest, sometimes for extended periods. when considering the adaptation of cells to physiological extremes of ph, temperature or pressure, it is generally assumed that evolution is driven toward opti ... | 2005 | 21676831 |
| tmp21, a novel mhc-i interacting protein, preferentially binds to +æ2-microglobulin-free mhc-i heavy chains. | mhc-i molecules play a critical role in immune surveillance against viruses by presenting peptides to cytotoxic t lymphocytes. although the mechanisms by which mhc-i molecules assemble and acquire peptides in the er are well characterized, how mhc-i molecules traffic to the cell surface remains poorly understood. to identify novel proteins that regulate the intracellular transport of mhc-i molecules, mhc-i-interacting proteins were isolated by affinity purification, and their identity was determ ... | 2011 | 21699748 |
| legionella pneumophilarequires polyamines for optimal intracellular growth. | the gram-negative intracellular pathogen legionella pneumophilareplicates in a membrane-bound compartment known as the legionella-containing vacuole (lcv), into which it abundantly releases its chaperonin, htpb. to determine whether htpb remains within the lcv or reaches the host cell cytoplasm, we infected u937 human macrophages and cho cells with l. pneumophilaexpressing a translocation reporter consisting of the bordetella pertussisadenylate cyclase fused to htpb. these infections led to incr ... | 2011 | 21742865 |
| candida glabrata pwp7p and aed1p are required for adherence to human endothelial cells. | candida glabrata owes its success as a pathogen, in part, to a large repertoire of adhesins present on the cell surface. our current knowledge of c. glabrata adhesins and their role in the interaction between host and pathogen is limited to work with only a single family of epithelial adhesins (epa proteins). here, we report on the identification and characterization of a family of glycosylphosphatidylinositol-anchored cell wall proteins in c. glabrata. these proteins are absent in both saccharo ... | 2011 | 21726406 |
| fly meets yeast: checking the correct orientation of cell division. | cell division is generally thought to be a process that produces an exact copy of the mother cell by precisely replicating its genomic dna, doubling organelles, and segregating them into two cells. many cell types from bacteria to human cells divide asymmetrically, however, to generate daughter cells with distinct characteristics. such asymmetric divisions are fundamental to the lifespan of a cell, to embryonic development, and to stem cell homeostasis. asymmetric division requires coordination ... | 2011 | 21705221 |
| targeting the regulation of androgen receptor signaling by the heat shock protein 90 cochaperone fkbp52 in prostate cancer cells. | drugs that target novel surfaces on the androgen receptor (ar) and/or novel ar regulatory mechanisms are promising alternatives for the treatment of castrate-resistant prostate cancer. the 52 kda fk506 binding protein (fkbp52) is an important positive regulator of ar in cellular and whole animal models and represents an attractive target for the treatment of prostate cancer. we used a modified receptor-mediated reporter assay in yeast to screen a diversified natural compound library for inhibito ... | 2011 | 21730179 |
| stereoselective biotransformation of timosaponin a-iii by saccharomyces cerevisiae. | bioconversion of timosaponin a-iii (ta-iii), one of the major steroidal saponins isolated from the rhizomes of anemarrhenae asphodeloides bunge (liliaceae), was investigated in saccharomyces cerevisiae. five bioconversion products, denoted compounds 2-6, were obtained. biotransformation metabolite 2 was a stereoisomer of taiii with a specific isotype f-ring and beta-ranged ch3-21, which rarely occurs in nature. the structure of 2 was elucidated by extensive spectroscopic analysis (h-h cosy, hsqc ... | 2011 | 21715964 |
| feedback regulation of ran gene expression by ran protein. | ran, an abundant gtpase that is highly conserved in eukaryotes from yeast to human, has been implicated in many aspects of nuclear structure and function. recently it is revealed that the ran gtpase can regulate the hemocytic phagocytosis of shrimp by interaction with myosin. however the regulation of ran gene expression remains unknown. in this study, the promoter of shrimp ran gene was identified which contained a typical tata box. the results showed that the shrimp ran protein was bound with ... | 2011 | 21741460 |
| saccharomyces as a vaccine against systemic aspergillosis: 'the friend of man' a friend again? | the mortality of clinical aspergillus infections necessitates consideration of the utility of a vaccine. we have found that saccharomyces species can act as a protective vaccine against a lethal systemic aspergillus infection, and describe experiments optimizing a subcutaneous regimen with killed yeast. three injections of 2.5 mg given a week apart, 2 weeks prior to challenge, consistently, significantly, provided survival protection and reduction of infection in organs in survivors. the protect ... | 2011 | 21825307 |
| purification and characterization of a novel aspartic protease from basidiomycetous yeast cryptococcus sp. s-2. | an aspartic protease (cap1) was purified from basidiomycetous yeast cryptococcus sp. s-2 (ferm abp-10961) using hitrap deae ff column and hitrap q hp column chromatography with azocasein as a substrate. cap1 has a molecular mass of 34kda on sds-page. it was stable up to 50-¦c with maximum activity at 30-¦c. maximum proteolytic activity was observed at ph 5.0. cap1 was stable in the ph range 3.0-7.0. its enzyme activity was strongly inhibited by pepstatin a, an inhibitor of aspartic proteases, in ... | 2011 | 21824815 |
| mediator and human disease. | since the identification of a metazoan counterpart to yeast mediator nearly 15 years ago, a convergent body of biochemical and molecular genetic studies have confirmed their structural and functional relationship as an integrative hub through which regulatory information conveyed by signal activated transcription factors is transduced to rna polymerase ii. nonetheless, metazoan mediator complexes have been shaped during evolution by substantive diversification and expansion in both the number an ... | 2011 | 21840410 |
| kif1a, an axonal transporter of synaptic vesicles, is mutated in hereditary sensory and autonomic neuropathy type 2. | hereditary sensory and autonomic neuropathy type ii (hsanii) is a rare autosomal-recessive disorder characterized by peripheral nerve degeneration resulting in a severe distal sensory loss. although mutations in fam134b and the hsn2 exon of wnk1 were associated with hsanii, the etiology of a substantial number of cases remains unexplained. in addition, the functions of wnk1/hsn2 and fam134b and their role in the peripheral nervous system remain poorly understood. using a yeast two-hybrid screen, ... | 2011 | 21820098 |
| cryptococcal titan cell formation is regulated by g-protein signaling in response to multiple stimuli. | the titan cell is a recently described morphological form of the pathogenic fungus cryptococcus neoformans. occurring during the earliest stages of lung infection, titan cells are 5-10 times larger than the normal yeast-like cells, thereby resisting engulfment by lung phagocytes and favoring persistence of infection. these enlarged cells exhibit altered capsule structure, thickened cell wall, increased ploidy, and resistance to nitrosative and oxidative stress. we demonstrate that two g protein- ... | 2011 | 21821718 |
| kif18b interacts with eb1 and controls astral microtubule length during mitosis. | regulation of microtubule (mt) dynamics is essential for proper spindle assembly and organization. kinesin-8 family members are plus-end-directed motors that modulate plus-end mt dynamics by acting as mt depolymerases or as mt plus-end capping proteins. in this paper, we show that the human kinesin-8 kif18b functions during mitosis to control astral mt organization. kif18b is a mt plus-tip-tracking protein that localizes to the nucleus in interphase and is enriched at astral mt plus ends during ... | 2011 | 21737685 |
| a comprehensive functional analysis of pten mutations: implications in tumor- and autism-related syndromes. | the pten (phosphatase and tensin homolog) phosphatase is unique in mammals in terms of its tumor suppressor activity, exerted by dephosphorylation of the lipid second messenger pip(3) (phosphatidylinositol 3,4,5-trisphosphate), which activates the phosphoinositide 3-kinase/akt/mtor (mammalian target of rapamycin) oncogenic pathway. loss-of-function mutations in the pten gene are frequent in human cancer and in the germline of patients with pten hamartoma tumor-related syndromes (phtss). in addit ... | 2011 | 21828076 |
| protein n-glycosylation of gastropods. | glycosylation plays an important role in several types of recognition processes associated with fertilisation and development, allergies, pathological events and cell death. whereas the amino acid sequence of a protein is fixed by the dna, the glycosylation abilities depend on enzymes and substrates currently present in the cell.during the last decades our knowledge on glycosylation - the structure of glycans as well as the corresponding biochemical pathways including the responsible enzymes - e ... | 2009 | 21686044 |
| glycosylation increases the thermostability of human aquaporin 10 protein. | human aquaporin10 (haqp10) is a transmembrane facilitator of both water and glycerol transport in the small intestine. this aquaglyceroporin is located in the apical membrane of enterocytes and is believed to contribute to the passage of water and glycerol through these intestinal absorptive cells. here we overproduced haqp10 in the yeast pichia pastoris and observed that the protein is glycosylated at asn-133 in the extracellular loop c. this finding confirms one of three predicted glycosylatio ... | 2011 | 21733844 |
| a yeast-based assay identifies drugs active against human mitochondrial disorders. | due to the lack of relevant animal models, development of effective treatments for human mitochondrial diseases has been limited. here we establish a rapid, yeast-based assay to screen for drugs active against human inherited mitochondrial diseases affecting atp synthase, in particular narp (neuropathy, ataxia, and retinitis pigmentosa) syndrome. this method is based on the conservation of mitochondrial function from yeast to human, on the unique ability of yeast to survive without production of ... | 2011 | 21715656 |
| bystander cytotoxicity in human medullary thyroid carcinoma cells mediated by fusion yeast cytosine deaminase and 5-fluorocytosine. | in our work, we have evaluated efficiency of gene-directed enzyme/prodrug therapy (gdept) based on combination of fusion yeast cytosine deaminase (ycd) and 5-fluorocytosine (5fc) on model human medullary thyroid carcinoma (mtc) cell line tt. we determined the efficiency of this gdept approach in suicide and bystander cytotoxicity induction. we have shown significant bystander effect in vitro and 5fc administration resulted in potent antitumor effect in vivo. furthermore, we have unraveled high e ... | 2011 | 21824724 |
| clustering of alpers disease mutations and catalytic defects in biochemical variants reveal new features of molecular mechanism of the human mitochondrial replicase, pol {gamma} | mutations in pol +¦ represent a major cause of human mitochondrial diseases, especially those affecting the nervous system in adults and in children. recessive mutations in pol +¦ represent nearly half of those reported to date, and they are nearly uniformly distributed along the length of the polg1 gene (human dna polymerase gamma mutation database); the majority of them are linked to the most severe form of polg syndrome, alpers-huttenlocher syndrome. in this report, we assess the structure-fu ... | 2011 | 21824913 |
| ca(v)2.3 ca2+ channel interacts with the g1-subunit of v-atpase. | calcium channels are essential in coupling action potential to signal transduction in cells. there are several types of calcium channels, which can be pharmacologically classified as l-, n-, p/q-, r- and t-type. but molecular basis of r-type channels is less clearly understood compared the other channel types. therefore the current study aims at understanding the molecular functions of r-type calcium channels by identifying interaction partners of the channel. | 2011 | 21691059 |
| antimicrobial screening of ethnobotanically important stem bark of medicinal plants. | the stem barks are the rich sources of tannins and other phenolic compounds. tannins inhibited the growth of various fungi, yeast, bacteria and virus. hence, ten stem barks of ethnomedicinally important plants were screened for antibacterial and antifungal activities against human pathogenic strains. | 2010 | 21808577 |
| hydroxylation of recombinant human collagen type i alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase. | abstract: | 2011 | 21702901 |
| opposing effects of glutamine and asparagine govern prion formation by intrinsically disordered proteins. | sequences rich in glutamine (q) and asparagine (n) residues often fail to fold at the monomer level. this, coupled to their unusual hydrogen-bonding abilities, provides the driving force to switch between disordered monomers and amyloids. such transitions govern processes as diverse as human protein-folding diseases, bacterial biofilm assembly, and the inheritance of yeast prions (protein-based genetic elements). a systematic survey of prion-forming domains suggested that q and n residues have d ... | 2011 | 21726811 |
| the ubc9 e2 sumo conjugating enzyme binds the pr-set7 histone methyltransferase to facilitate target gene repression. | pr-set7/set8/kmt5a is a chromatin-modifying enzyme that specifically monomethylates lysine 20 of histone h4 (h4k20me1). in this study we attempted to identify pr-set7-interacting proteins reasoning that these proteins would provide important insights into the role of pr-set7 in transcriptional regulation. using an unbiased yeast two-hybrid approach, we discovered that pr-set7 interacts with the ubc9 e2 sumo conjugating enzyme. this interaction was confirmed in human cells and we demonstrated tha ... | 2011 | 21829513 |
| sirt6 promotes dna repair under stress by activating parp1. | sirtuin 6 (sirt6) is a mammalian homolog of the yeast sir2 deacetylase. mice deficient for sirt6 exhibit genome instability. here, we show that in mammalian cells subjected to oxidative stress sirt6 is recruited to the sites of dna double-strand breaks (dsbs) and stimulates dsb repair, through both nonhomologous end joining and homologous recombination. our results indicate that sirt6 physically associates with poly[adenosine diphosphate (adp)-ribose] polymerase 1 (parp1) and mono-adp-ribosylate ... | 2011 | 21680843 |
| [new nutrient medium for the cultivation and isolation of the plague microbe chds-37 as an element of the mobilization reserve of specialized antiepidemic teams of the russian inspectorate for the protection of consumer rights and human welfare]. | a new nutrient medium has been designed to culture and isolate the plague microbe chds-37 on the basis of the pancreatic digest of baker's yeast. the results of laboratory tests of the designed medium, by using 10 plague microbe strains and those of approval during the tactical and special training of a specialized antiepidemic team (saet), suggest that the medium has some advantage over reference media and creates prerequisites for being incorporated into the mobilization reserve of a saet. | 2011 | 21735682 |
| isolation of compensatory inhibitor domain mutants to novel activation domain variants using the split-ubiquitin screen. | the control of transcription factor function plays an important role in the development of many processes in eukaryotes, such as drug resistance in fungi and human tumours undergoing chemotherapy. detailed molecular mapping of the interactions between transcription factors and their protein partners can give important information about their mechanisms of action and reveal potential therapeutic targets. we devised a genetic screening system for mapping the interaction site between the saccharomy ... | 2011 | 21732556 |
| three "hotspots" important for adenosine a(2b) receptor activation: a mutational analysis of transmembrane domains 4 and 5 and the second extracellular loop. | g protein-coupled receptors (gpcrs) are a major drug target and can be activated by a range of stimuli, from photons to proteins. despite the progress made in the last decade in molecular and structural biology, their exact activation mechanism is still unknown. here we describe new insights in specific regions essential in adenosine a(2b) receptor activation (a(2b)r), a typical class a gpcr. we applied unbiased random mutagenesis on the middle part of the human adenosine a(2b)r, consisting of t ... | 2011 | 21818573 |
| [human arginase i from the recombinant yeast hansenula polymorpha: isolation and characterization of the enzyme]. | purified human arginase i preparations homogeneous in sds-paag test were obtained by the affinity chromatography on the synthesized sorbent l-arginine-macroporous glass. some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and ph-stability, temperature- and ph-optima of the enzyme. the influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was stu ... | 2010 | 21805858 |
| candida albicans hgt1p, a multifunctional evasion molecule: complement inhibitor, cr3 analogue, and human immunodeficiency virus-binding molecule. | background.ôçâthe complement system is tightly controlled by several regulators. two of these, factor h (fh) and c4b-binding protein (c4bp), can be acquired by pathogens conveying resistance to complement attack. the aim of the study was to characterize the fh binding molecule of candida albicans, a potentially life-threatening yeast. methods.ôçâthe gene coding for this molecule was identified by probing an expression library and homozygous deletion mutants of the respective gene were constructe ... | 2011 | 21844307 |
| bioorthogonal chemical reporters for analyzing protein lipidation and lipid trafficking. | protein lipidation and lipid trafficking control many key biological functions in all kingdoms of life. the discovery of diverse lipid species and their covalent attachment to many proteins has revealed a complex and regulated network of membranes and lipidated proteins that are central to fundamental aspects of physiology and human disease. given the complexity of lipid trafficking and the protein targeting mechanisms involved with membrane lipids, precise and sensitive methods are needed to mo ... | 2011 | 21675729 |
| An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway. | In eukaryotic cells, different RNA species are exported from the nucleus via specialized pathways. The mRNA export machinery is highly integrated with mRNA processing, and includes a different set of nuclear transport adaptors as well as other mRNA binding proteins, RNA helicases, and NPC-associated proteins. The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, a widespread and neglected human disease which is endemic to Latin America. Gene expression in Trypanosoma ... | 2011 | 21687672 |
| A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor. | The TRAPP vesicle-tethering complex consists of more than 10 distinct polypeptides and is involved in protein transport. Using the C2 subunit as bait we identified SPATA4, a spermatocyte-specific protein of unknown function, as an interacting partner in a yeast two hybrid screen. Further studies indicate SPATA4 interacts with the C2 portion of the TRAPP complex. SPATA4 fractionates with both cytosolic and nuclear fractions suggesting it may have several distinct functions. SPATA4 is one of only ... | 2011 | 21827752 |
| Dipoid-specific genome stability genes of S. cerevisiae: genomic screen reveals haploidization as an escape from persisting DNA rearrangement stress. | Maintaining a stable genome is one of the most important tasks of every living cell and the mechanisms ensuring it are similar in all of them. The events leading to changes in DNA sequence (mutations) in diploid cells occur one to two orders of magnitude more frequently than in haploid cells. The majority of those events lead to loss of heterozygosity at the mutagenesis marker, thus diploid-specific genome stability mechanisms can be anticipated. In a new global screen for spontaneous loss of fu ... | 2011 | 21695049 |
| p53 gene repair with zinc finger nucleases optimised by yeast 1-hybrid and validated by solexa sequencing. | the tumor suppressor gene p53 is mutated or deleted in over 50% of human tumors. as functional p53 plays a pivotal role in protecting against cancer development, several strategies for restoring wild-type (wt) p53 function have been investigated. in this study, we applied an approach using gene repair with zinc finger nucleases (zfns). we adapted a commercially-available yeast one-hybrid (y1h) selection kit to allow rapid building and optimization of 4-finger constructs from randomized pcr libra ... | 2011 | 21695267 |
| species of candida isolated from anatomically distinct sites in military personnel in cuiabá, mato grosso, brazil. | some fungi are natural inhabitants of the human body but may result in disease when conditions are conducive to their development. yeast infections are common and often occur in the skin and mucous membranes; however emerging species have changed this epidemiological profile. the ability to colonize different anatomical sites has been associated with the pathogenicity of candida when environmental conditions are particularly favorable. in the case of hot, humid climates, the attrition suffered b ... | 2011 | 21987132 |
| ATM controls meiotic double-strand-break formation. | In many organisms, developmentally programmed double-strand breaks (DSBs) formed by the SPO11 transesterase initiate meiotic recombination, which promotes pairing and segregation of homologous chromosomes. Because every chromosome must receive a minimum number of DSBs, attention has focused on factors that support DSB formation. However, improperly repaired DSBs can cause meiotic arrest or mutation; thus, having too many DSBs is probably as deleterious as having too few. Only a small fraction of ... | 2011 | 22002603 |
| A role for SUMO in nucleotide excision repair. | The two Siz/PIAS SUMO E3 ligases Siz1 and Siz2 are responsible for the vast majority of sumoylation in Saccharomyces cerevisiae. We found that siz1? siz2? mutants are sensitive to ultra-violet (UV) light. Epistasis analysis showed that the SIZ genes act in the nucleotide excision repair (NER) pathway, and suggested that they participate both in global genome repair (GGR) and in the Rpb9-dependent subpathway of transcription-coupled repair (TCR), but have minimal role in Rad26-dependent TCR. Quan ... | 2011 | 21968059 |
| The plasma membrane-associated GTPase Rin interacts with the dopamine transporter and is required for protein kinase C-regulated dopamine transporter trafficking. | Dopaminergic signaling and plasticity are essential to numerous CNS functions and pathologies, including movement, cognition, and addiction. The amphetamine- and cocaine-sensitive dopamine (DA) transporter (DAT) tightly controls extracellular DA concentrations and half-life. DAT function and surface expression are not static but are dynamically modulated by membrane trafficking. We recently demonstrated that the DAT C terminus encodes a PKC-sensitive internalization signal that also suppresses b ... | 2011 | 21957239 |
| MIF from mussel: Coding sequence, phylogeny, polymorphism, 3D model and regulation of expression. | Three macrophage migration inhibitory factor (MIF)-related sequences were identified from a Mytilus galloprovincialis EST library. The consensus sequence included a 5'-UTR of 32 nucleotides, the complete ORF of 345 nucleotides, and a 3'-UTR of 349 nucleotides. As for other MIFs, M. galloprovincialis ORF does not include any signal or C-terminus extensions. The translated sequence of 115 amino acids possesses a molecular mass of 12,681.4, a pI of 6.27 and a stability index of 21.48. Its 3D struct ... | 2011 | 22085783 |
| Antagonists of anaphase-promoting complex (APC)-2-cell cycle and apoptosis regulatory protein (CARP)-1 interaction are novel regulators of cell growth and apoptosis. | CARP-1/CCAR1, a perinuclear phosphoprotein, is a regulator of cell growth and apoptosis signaling. Although CARP-1 is a regulator of chemotherapy-dependent apoptosis, it is also a part of the NF-?B proteome and a co-activator of steroid/thyroid nuclear receptors as well as ß-catenin signaling. Our yeast two-hybrid screen revealed CARP-1 binding with the anaphase-promoting complex/cyclosome E3 ubiquitin ligase component APC-2 protein. CARP-1 also binds with anaphase-promoting complex/cyclosome co ... | 2011 | 21903591 |
| Biochemical characterization of Warsaw Breakage Syndrome helicase. | Mutations in the human ChlR1 gene are associated with a unique genetic disorder known as Warsaw Breakage Syndrome characterized by cellular defects in sister chromatid cohesion and hypersensitivity to agents that induce replication stress. A role of ChlR1 helicase in sister chromatid cohesion was first evidenced by studies of the yeast homolog Chl1p; however, its cellular functions in DNA metabolism are not well understood. We have carefully examined the DNA substrate specificity of purified r ... | 2011 | 22102414 |
| toward an understanding of the protein interaction network of the human liver. | proteome-scale protein interaction maps are available for many organisms, ranging from bacteria, yeast, worms and flies to humans. these maps provide substantial new insights into systems biology, disease research and drug discovery. however, only a small fraction of the total number of human protein-protein interactions has been identified. in this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human ... | 2011 | 21988832 |
| air1 zinc knuckles 4 and 5 and a conserved iwrxy motif are critical for the function and integrity of the trf4/5-air1/2-mtr4 polyadenylation (tramp) rna quality control complex. | in saccharomyces cerevisiae, non-coding rnas, including cryptic unstable transcripts (cuts), are subject to degradation by the exosome. the trf4/5-air1/2-mtr4 polyadenylation (tramp) complex in s. cerevisiae is a nuclear exosome cofactor that recruits the exosome to degrade rnas. trf4/5 are poly(a) polymerases, mtr4 is an rna helicase, and air1/2 are putative rna-binding proteins that contain five cchc zinc knuckles (znks). one central question is how the tramp complex, especially the air1/2 pro ... | 2011 | 21878619 |
| Fulminant Candidemia Diagnosed by Prompt Detection of Pseudohyphae in a Peripheral Blood Smear. | A 77-year-old man treated with prednisolone for pemphigus developed severe sepsis by Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Several antibiotics were administered. A peripheral blood smear showed growth of a large number of yeast extending pseudohyphae which could be seen both inside and outside of leucocytes. Antifungal agents were added immediately; however, he did not recover. Several days later, blood culture showed Candida albicans septicemia. The autopsy rev ... | 2011 | 22173051 |
| plasticity and diversity of trna anticodon determinants of substrate recognition by eukaryotic a37 isopentenyltransferases. | the n(6)-(isopentenyl)adenosine (i(6)a) modification of some trnas at position a37 is found in all kingdoms and facilitates codon-specific mrna decoding, but occurs in different subsets of trnas in different species. here we examine yeasts' trna isopentenyltransferases (i.e., dimethylallyltransferase, dmatase, members of the δ(2)-isopentenylpyrophosphate transferase, ippt superfamily) encoded by tit1(+) in schizosaccharomyces pombe and mod5 in saccharomyces cerevisiae, whose homologs are escheri ... | 2011 | 21873461 |
| inhibition of homologous recombination by the pcna-interacting protein pari. | inappropriate homologous recombination (hr) causes genomic instability and cancer. in yeast, the uvrd family helicase srs2 is recruited to sites of dna replication by sumo-modified pcna, where it acts to restrict hr by disassembling toxic rad51 nucleofilaments. how human cells control recombination at replication forks is unknown. here, we report that the protein pari, containing a uvrd-like helicase domain, is a pcna-interacting partner required for preservation of genome stability in human and ... | 2011 | 22153967 |
| Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. | In eukaryotes, the essential dimeric molecular chaperone Hsp90 is required for the activation and maturation of specific substrates such as steroid hormone receptors, tyrosine kinases and transcription factors. Hsp90 is involved in the establishment of cancer and has become an attractive target for drug design. Here we present a structural characterization of the complex between Hsp90 and the tumor suppressor p53, a key mediator of apoptosis whose structural integrity is crucial for cell-cycle c ... | 2011 | 21892170 |
| probing the force generation and stepping behavior of cytoplasmic dynein. | cytoplasmic dynein, which is the largest and arguably the most complex cytoskeletal motor protein, plays fundamental roles during cell division, nuclear positioning, and organelle and mrna transport, by generating force and movement toward the minus ends of microtubules. consequently, dynein is central to many physiological processes, and its dysfunction is implicated in human diseases. however, the molecular mechanism by which dynein produces force and movement remains poorly understood. here, ... | 2011 | 21909883 |
| mechanism of start site selection by rna polymerase ii: interplay between tfiib and ssl2/xpb helicase subunit of tfiih. | tfiib is essential for transcription initiation by rna polymerase ii. tfiib also cross-links to terminator regions and is required for gene loops that juxtapose promoter-terminator elements in a transcription-dependent manner. the saccharomyces cerevisiae sua7-1 mutation encodes an altered form of tfiib (e62k) that is defective for both start site selection and gene looping. here we report the isolation of an ssl2 mutant, encoding an altered form of tfiih, as a suppressor of the cold-sensitive g ... | 2012 | 22081613 |
| mutations in the dimer interface of dihydrolipoamide dehydrogenase promote site-specific oxidative damages in yeast and human cells. | dihydrolipoamide dehydrogenase (dld) is a multifunctional protein well characterized as the e3 component of the pyruvate dehydrogenase and α-ketoglutarate dehydrogenase complexes. previously, conditions predicted to destabilize the dld dimer revealed that dld could also function as a diaphorase and serine protease. however, the relevance of these cryptic activities remained undefined. we analyzed human dld mutations linked to strikingly different clinical phenotypes, including e340k, d444v, r447 ... | 2011 | 21930696 |
| recql5 cooperates with topoisomerase ii alpha in dna decatenation and cell cycle progression. | dna decatenation mediated by topoisomerase ii is required to separate the interlinked sister chromatids post-replication. sgs1, a yeast homolog of the human recq family of helicases interacts with topoisomerase ii and plays a role in chromosome segregation, but this functional interaction has yet to be identified in higher organisms. here, we report a physical and functional interaction of topoisomerase iiα with recql5, one of five mammalian recq helicases, during dna replication. direct interac ... | 2011 | 22013166 |
| Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy. | Autophagy, the primary recycling pathway of cells, plays a critical role in mitochondrial quality control under normal growth conditions and in the response to cellular stress. The Hsp90-Cdc37 chaperone complex coordinately regulates the activity of select kinases to orchestrate many facets of the stress response. Although both maintain mitochondrial integrity, the relationship between Hsp90-Cdc37 and autophagy has not been well characterized. Ulk1, one of the mammalian homologs of yeast Atg1, i ... | 2011 | 21855797 |
| Functional links between Aß toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. | Aß (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aß toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aß was previously unknown. The factors identified in yeast modified Aß toxicity in glutamatergic neurons of Caenorhabditis elega ... | 2011 | 22033521 |
| comparison of humoral immune responses to epstein-barr virus and kaposi's sarcoma-associated herpesvirus using a viral proteome microarray. | epstein-barr virus (ebv) is a ubiquitous herpesvirus, and kaposi's sarcoma-associated herpesvirus (kshv) has a restricted seroprevalence. both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (hiv-1). | 2011 | 21990424 |
| Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex. | Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated w ... | 2011 | 22209934 |
| flavivirus ns3 and ns5 proteins interaction network: a high-throughput yeast two-hybrid screen. | the genus flavivirus encompasses more than 50 distinct species of arthropod-borne viruses, including several major human pathogens, such as west nile virus, yellow fever virus, japanese encephalitis virus and the four serotypes of dengue viruses (denv type 1-4). each year, flaviviruses cause more than 100 million infections worldwide, some of which lead to life-threatening conditions such as encephalitis or haemorrhagic fever. among the viral proteins, ns3 and ns5 proteins constitute the major e ... | 2011 | 22014111 |
| Expression of Multisubunit Proteins in Leishmania tarentolae. | Heterologous gene expression in mammalian cells is the first choice for the production of recombinant proteins when posttranslational modifications affect the biological activity of target proteins. However, the expression efficiency of mammalian cells is relatively low compared to other expression systems, such as Escherichia coli or yeast. Recently, a novel protein expression system based on Leishmania tarentolae, a protozoan parasite of gecko, was developed. This system allows not only easy h ... | 2012 | 22160906 |
| Copper Chaperone-Dependent and -Independent Activation of Three Copper-Zinc Superoxide Dismutase Homologs Localized in Different Cellular Compartments in Arabidopsis. | Superoxide dismutases (SODs) are important antioxidant enzymes that catalyze the disproportionation of superoxide anion to O2 and H2O2 to guard cells against superoxide toxicity. The major pathway for activation of Cu-Zn SOD (CuZnSOD; CSD) involves a Cu chaperone for SOD (CCS) and an additional minor CCS-independent pathway reported in mammals. We characterized the CCS-dependent and -independent activation pathways for three CSDs localized in different cellular compartments in Arabidopsis thalia ... | 2011 | 22186608 |