| a new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples. | a microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. it involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. captured cells are then cultivated in situ by infusing nutritionally-rich medium. the system was validated by the direct one-step detection of salmonella typhimurium in undilut ... | 2017 | 28626552 |
| aerobic biogenesis of selenium nanoparticles by enterobacter cloacae z0206 as a consequence of fumarate reductase mediated selenite reduction. | in the present study, we examined the ability of enterobacter cloacae z0206 to reduce toxic sodium selenite and mechanism of this process. e. cloacae z0206 was found to completely reduce up to 10 mm selenite to elemental selenium (se°) and form selenium nanoparticles (senps) under aerobic conditions. the selenite reducing effector of e. cloacae z0206 cell was to be a membrane-localized enzyme. itraq proteomic analysis revealed that selenite induced a significant increase in the expression of fum ... | 2017 | 28607388 |
| population pharmacokinetics of tigecycline in critically ill patients with severe infections. | we sought to describe the population pharmacokinetics of tigecycline in critically ill patients and to determine optimized dosing regimens of tigecycline for different bacterial infections. this prospective study included 10 critically ill patients given a standard dose of tigecycline. blood samples were collected during one dosing interval and were analyzed using validated chromatography. population pharmacokinetics and monte carlo dosing simulations were undertaken using pmetrics. three target ... | 2017 | 28607024 |
| fri-2 carbapenemase-producing enterobacter cloacae complex in the uk. | detection of rarer carbapenemases is challenging, as it requires molecular assays with comprehensive coverage or the use of phenotypic methods for the detection of carbapenemase activity. we describe a new class a carbapenemase, fri-2, in an enterobacter cloacae complex isolate following implementation of an in-house multiplex pcr for the detection of 'rare' class a carbapenemases. | 2017 | 28605515 |
| draft genome sequences of enterobacter cloacae strains caprex e7 and caprex e2-2. | enterobacter cloacae strains caprex e7 and caprex e2-2 were isolated from ghanaian yams at a london market. the draft genome sequences indicate that the two strains are similar, with genomes of 5,042,838 and 5,039,930 bp and 56.19% and 55.05% g+c content, respectively. both strains encoded three different β-lactamases, including one of the ampc family. | 2017 | 28596406 |
| genetic and biochemical characterisation of ctx-m-37 extended-spectrum β-lactamase from an enterobacter cloacae clinical isolate from mongolia. | the aims of this study were to determine the resistance level of a blactx-m-37-carrying enterobacter cloacae isolate from mongolia, to analyse kinetic parameters of the purified enzyme and to compare the genetic environment of the gene. | 2017 | 28587869 |
| microbial decolorization and detoxification of emerging environmental pollutant: cosmetic hair dyes. | since the usage of hair dyes has increased in recent time, the removal of residual dye from environment is also an emerging issue. hair dye contains mixture of chemicals including genotoxic chemical, p-phenylenediamine (p-pd or ppd). the present study reports bioremediation of hair dye using bacteria isolated from saloon effluent. sugarcane bagasse powder (sbp) was used as a source of nutrient and surface for bacterial growth. the 16s rdna sequencing confirmed the isolate as enterobacter cloacae ... | 2017 | 28586750 |
| enterobacter cloacae-related necrotizing fasciitis after peritoneal dialysis in delayed graft function: a case report. | necrotizing fasciitis is an uncommon soft-tissue infection that involves the superficial fascia, subcutaneous fat, and deep fascia. herein, we report the first case of enterobacter cloacae-related necrotizing fasciitis after peritoneal dialysis in delayed graft function. | 2017 | 28583554 |
| mechanism-informed refinement reveals altered substrate-binding mode for catalytically competent nitroreductase. | nitroreductase (nr) from enterobacter cloacae reduces diverse nitroaromatics including herbicides, explosives, and prodrugs, and holds promise for bioremediation, prodrug activation, and enzyme-assisted synthesis. we solved crystal structures of nr complexes with bound substrate or analog for each of its two half-reactions. we complemented these with kinetic isotope effect (kie) measurements elucidating h-transfer steps essential to each half-reaction. kies indicate hydride transfer from nadh to ... | 2017 | 28578873 |
| multilocus sequence typing of carbapenemase-producing enterobacter cloacae bloodstream isolates between 2002 and 2013 in a taiwanese university hospital. | | 2017 | 28576741 |
| mcr-1 plasmid-mediated colistin resistance gene detection in an enterobacter cloacae clinical isolate in france. | | 2017 | 28576739 |
| outbreak of ctx-m-15-producing enterobacter cloacae associated with therapeutic beds and syphons in an intensive care unit. | an outbreak of extended-spectrum β-lactamase-producing enterobacter cloacae (esbl-ecl) occurred in our intensive care unit (icu) and involved 18 patients (8 infected and 10 colonized). the mean age of patients was 69 years, and all infected patients had underlying medical conditions. within hours' recognition of the spread of esbl-ecl, the infection control team requested for staff education, reinforcement of infection control measures, and environmental screening. new transmissions were observe ... | 2017 | 28571981 |
| total hip prosthesis complication, periprosthetic infection with external fistulizing due to enterobacter cloacae complex multiple drugs resistance: a clinical case report. | the enterobacter cloacae is a microorganism found in the intestinal flora of the majority of animals, including humans. primary infections caused by e. cloacae are rare in immunocompetent patients, but are very common in hospital settings in newborns and immunocompromised patients, and can be aggravated by the insurgence of antibiotic resistance. the incidence of periprosthetic hip infections is just below 2%. | 2017 | 28558347 |
| outbreak of enterobacter cloacae with new delhi metallo-β-lactamase (ndm)-1: challenges in epidemiological investigation and environmental decontamination. | | 2017 | 28552405 |
| multiple brain abscesses due to enterobacter cloacae in an immune-competent child. | brain abscesses due to enterobacteriaceae in immune-competent children are rare, and those due to enterobacter cloacae are even rarer. we report an interesting case of community-acquired e. cloacae neuroinfection resulting in multiple brain abscesses in a young child with no underlying risk-factors. a 10 year-old-boy presented with low-grade fever, headache, neck pain and progressive deterioration of sensorium. on examination, he was conscious but drowsy with photophobia, normal fundii, meningea ... | 2017 | 28545899 |
| epidemiological, clinical and microbiological characteristics of patients with post-traumatic osteomyelitis of limb fractures in southwest china: a hospital-based study. | objective to determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. methods human subject research was performed using institutional review board approved protocols. a retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from january 1, 2012 to d ... | 2017 | 28540152 |
| genomic epidemiology of global vim-producing enterobacteriaceae. | international data on the molecular epidemiology of enterobacteriaceae with vim carbapenemases are limited. | 2017 | 28520983 |
| bioremoval of lead using pennisetum purpureum augmented with enterobacter cloacae-vitpasj1: a pot culture approach. | lead is a toxic heavy metal discharged into the ecosystem from various industries. biological remediation strategies have been effective in the bioremoval of lead. in our current study, a phytobacterial system using pennisetum purpureum along with lead-resistant bacterium (lrb) was employed for the uptake of lead. the lrb was obtained from lead-contaminated sites. the isolate vitpasj1 was found to be highly tolerant to lead and was identified as an effective plant growth-promoting bacterium. the ... | 2017 | 28509939 |
| a metabonomic analysis on the response of enterobacter cloacae from coastal outfall for land-based pollutant under phoxim stress. | enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. the secretion of extended-spectrum β-lactamases (esbls) and cephalosporinase (ampc) endows e. cloacae with strong drug resistance. in a previous study by our group, protein expression of e. cloacae under phoxim stress was measured by two-dimensional electrophoresis. here, nuclear magnetic resonance was used to detect differences in e. cloacae metabonomics when under phoxim stress. we dete ... | 2017 | 28508092 |
| applications of microalgal biofilms for wastewater treatment and bioenergy production. | microalgae have shown clear advantages for the production of biofuels compared with energy crops. apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (c, n, and p), heavy metals and micropollutants, and they do not compete with crops for arable lands. however, fundamental barriers to the industrial application of microalgae for biofuel production ... | 2017 | 28491136 |
| evaluation of the carbapenem inactivation method (cim) for detecting carbapenemase activity in enterobacteria. | the objective of this study was to evaluate the accuracy of the cim test in the detection of carbapenemase activity in 124 strains of enterobacteriaceae. | 2017 | 28476242 |
| clinical and molecular epidemiology of carbapenem-resistant enterobacteriaceae among adult inpatients in singapore. | since 2010, the incidence of carbapenem-resistant enterobacteriaceae (cre) has been increasing in singapore. we analyzed the clinical and molecular epidemiology of cre among adult inpatients in singapore. | 2017 | 28475792 |
| usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify pathogens, including polymicrobial samples, directly from blood culture broths. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (tof-ms) is now widely used to detect pathogens in clinical settings in japan. here, we report the ability of tof-ms to detect bacteria from blood culture (bc) broths, and compare the efficacy of tof-ms to that of conventional culture methods. bacteria were correctly detected from 63 monomicrobial samples within 80 minutes; results matched those obtained by conventional bc methods, although the conventional methods took ... | 2017 | 28458566 |
| transfer of kpc-2 carbapenemase from klebsiella pneumoniae to enterobacter cloacae in a patient receiving meropenem therapy. | the horizontal transfer of a plasmid bearing the blakpc-2 gene from k. pneumoniae to e. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. this finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant gram-negative pathogens. | 2017 | 28442305 |
| novel monoclonal antibodies against stx1d and 1e and their use for improving immunoassays. | shiga toxins (stxs) are major causative agents for bloody diarrhea and hemolytic uremic syndrome, a life-threatening disease in humans. no effective treatment is available. early detection of stxs in clinical samples is critical for disease management. as bacteria evolve, new stxs are produced; therefore, methods used to identify them need to be improved as well. in this study, new monoclonal antibodies (mabs) against stx1d and 1e were developed and used to improve a commercial stx1 kit. incorpo ... | 2017 | 28434982 |
| a comparison of molecular typing methods applied to enterobacter cloacae complex: hsp60 sequencing, rep-pcr, and mlst. | molecular typing using repetitive sequenced-based pcr (rep-pcr) and hsp60 sequencing were applied to a collection of diverse enterobacter cloacae complex isolates. to determine the most practical method for reference laboratories, we analyzed 71 e. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. while rep-pcr was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus seq ... | 2017 | 28428984 |
| allodemic distribution of plasmids co-harbouring blactx-m-15/aac(6')-ib-cr/qnrb in klebsiella pneumoniae is the main source of extended-spectrum β-lactamases in uruguay's paediatric hospital. | this study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (esbl)-producing enterobacteria (epe) in uruguay's paediatric hospital. | 2017 | 28419869 |
| carbapenem-resistant enterobacter cloacae in patients from the us veterans health administration, 2006-2015. | we analyzed carbapenem-resistant enterobacteriaceae (cre) trends among patients from the us veterans health administration (vha). after the emergence of cre in the eastern united states, resistance rates remained stable in klebsiella pneumoniae but increased in enterobacter cloacae complex, suggesting a "second epidemic". vha offers a vantage point for monitoring nationwide cre trends. | 2017 | 28418318 |
| oxa-48-like carbapenemases in the uk: an analysis of isolates and cases from 2007 to 2014. | oxa-48-like carbapenemases have spread worldwide since 2001. we analysed patient and microbiological data for uk isolates with these enzymes as confirmed by the national reference laboratory from november 2007 to december 2014. | 2017 | 28199647 |
| factors associated with carriage of carbapenem-non-susceptible enterobacteriaceaein north-eastern france and outcomes of infected patients. | carbapenems are frequently used as a last resort to treat infections caused by multidrug-resistant gram-negative organisms, thus carbapenem-non-susceptible enterobacteriaceae (cnse) is an emerging health threat. | 2017 | 28158526 |
| highly regioselective glycosylation of xylosyl-containing taxanes by enterobacter cloaceae. | three new diglycoside taxanes (4-6) containing an additional α-d-glucose unit were obtained via enterobacter cloaceae-mediated, highly regioselective glycosylations at oh-4 of the xylosyl moieties in 7-xylosyl taxanes (1-3). their structures were determined on the basis of extensive spectroscopic analysis and chemical reactivity. this is the first report of an enzymatic elongation of the saccharide chain in taxanes, and the glycosylation reaction is the first described example of a transformatio ... | 2013 | 23178221 |
| accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae. | a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ... | 2006 | 28700883 |
| combination antimicrobial therapy for bacterial infections : guidelines for the clinician. | therapy with antimicrobial combinations has been used as long as antimicrobials have been available. combinations of antibiotics are often used to take advantage of different mechanisms of action and/or toxicity profiles. well established indications for combination antimicrobial therapy include: (a) empirical treatment of life-threatening infections; (b) treatment of polymicrobial infections; (c) prevention of the emergence of bacterial resistance; and (d) for synergism. disadvantages of combin ... | 1996 | 28593588 |